Concise Encyclopedia of Plant Pathology

Concise Encyclopedia
of Plant Pathology
FOOD PRODUCTS PRESS
Crop Science
Amarjit S. Basra, PhD
Senior Editor
Heterosis and Hybrid Seed Production in Agronomic Crops edited by Amarjit S. Basra
Intensive Cropping: Efficient Use of Water, Nutrients, and Tillage by S. S. Prihar,
P. R. Gajri, D. K. Benbi, and V. K. Arora
Physiological Bases for Maize Improvement edited by Mara E. Otegui and Gustavo
A. Slafer
Plant Growth Regulators in Agriculture and Horticulture: Their Role and Commercial Uses
edited by Amarjit S. Basra
Crop Responses and Adaptations to Temperature Stress edited by Amarjit S. Basra
Plant Viruses As Molecular Pathogens by Jawaid A. Khan and Jeanne Dijkstra
In Vitro Plant Breeding by Acram Taji, Prakash P. Kumar, and Prakash Lakshmanan
Crop Improvement: Challenges in the Twenty-First Century edited by Manjit S. Kang
Barley Science: Recent Advances from Molecular Biology to Agronomy of Yield and Quality
edited by Gustavo A. Slafer, Jos Luis Molina-Cano, Roxana Savin, Jos Luis Araus,
and Ignacio Romagosa
Tillage for Sustainable Cropping by P. R. Gajri, V. K. Arora, and S. S. Prihar
Bacterial Disease Resistance in Plants: Molecular Biology and Biotechnological Applications
by P. Vidhyasekaran
Handbook of Formulas and Software for Plant Geneticists and Breeders edited by Manjit
S. Kang
Postharvest Oxidative Stress in Horticultural Crops edited by D. M. Hodges
Encyclopedic Dictionary of Plant Breeding and Related Subjects by Rolf H. G. Schlegel
Handbook of Processes and Modeling in the Soil-Plant System edited by D. K. Benbi
and R. Nieder
The Lowland Maya Area: Three Millennia at the Human-Wildland Interface edited
by A. Gmez-Pompa, M. F. Allen, S. Fedick, and J. J. Jimnez-Osornio
Biodiversity and Pest Management in Agroecosystems, Second Edition by Miguel A. Altieri
and Clara I. Nicholls
Plant-Derived Antimycotics: Current Trends and Future Prospects edited by Mahendra Rai
and Donatella Mares
Concise Encyclopedia of Temperate Tree Fruit edited by Tara Auxt Baugher
and Suman Singha
Landscape Agroecology by Paul A Wojkowski
Molecular Genetics and Breeding of Forest Trees edited by Sandeep Kumar and Matthias
Fladung
Concise Encylcopedia of Plant Pathology by P. Vidhyasekaran
Testing of Genetically Modified Organisms in Foods edited by Farid E. Ahmed
Concise Encyclopedia of Bioresource Technology edited by Ashok Pandey
Agrometeorology: Principles and Applications of Climate Studies in Agriculture by Aarpal
S. Mavi and Graeme J. Tupper
Concise Encyclopedia
of Plant Pathology
P. Vidhyasekaran, PhD, FNA
Food Products Press

The Haworth Reference Press
Imprints of The Haworth Press, Inc.
New York London Oxford
Published by
Food Products Press and The Haworth Reference Press, imprints of The Haworth Press, Inc.,
10 Alice Street, Binghamton, NY 13904-1580.
2004 by The Haworth Press, Inc. All rights reserved. No part of this work may be reproduced or
utilized in any form or by any means, electronic or mechanical, including photocopying, microfilm,
and recording, or by any information storage and retrieval system, without permission in writing
from the publisher. Printed in the United States of America.
Cover design by Marylouise E. Doyle.
Library of Congress Cataloging-in-Publication Data
Vidhyasekaran, P.
Concise encyclopedia of plant pathology / P. Vidhyasekaran.
p. cm.
Includes bibliographical references and index.
ISBN 1-56022-942-X (hard cover : alk. paper)ISBN 1-56022-943-8 (pbk. : alk. paper)
1. Plant diseasesEncylopedias. I. Title.
SB728.V525 2003
632'.3'03dc21
2003002492
CONTENTS
Foreword
xv
Sally A. Miller
Preface
Chapter 1. Birth and Development of Plant Pathology
History
Modern Plant Pathology
xvii
1
1
4
CAUSAL ORGANISMS
Chapter 2. Bacteria
Structure of Bacterial Cells
Reproduction of Bacteria
Nomenclature of Bacterial Pathogens
Classification of Plant Bacterial Pathogens
Systematic Position of Crop Bacterial Pathogens
11
11
13
14
16
18
Chapter 3. Fungi, Including Chromista and Protozoa

Three Kingdoms Containing Fungi: Fungi, Chromista,
and Protozoa
Oomycota
Protozoa
Phyla in the Kingdom Fungi
Ascomycota
Basidiomycota
Chytridiomycota
Zygomycota
Mitosporic Fungi
Systematic Positions of Crop Fungal Pathogens
(Including Chromista and Protozoa)
29
29
30
32
33
34
40
45
46
47
Chapter 4. Parasitic Flagellate Protozoa

Phloem Necrosis of Coffee
Hartrot of Coconut
Marchitez Disease of Oil Palm
71
71
72
72
48
Chapter 5. Parasitic Green Algae

Economic Importance
Disease Symptoms
Life Cycle
Disease Management
73
73
73
74
75
Chapter 6. Parasitic Higher Plants

Striga
Orobanche
Loranthus
Cassytha
Cuscuta
77
77
79
80
80
80
Chapter 7. Parasitic Nematodes

Structure of Nematodes
Plant Parasitic Nematodes
Disease Symptoms
Management of Parasitic Nematodes
83
83
84
90
92
Chapter 8. Phytoplasmas and Spiroplasmas

Phytoplasmas
Spiroplasmas
99
99
104
Chapter 9. Viroids
Structure of Viroids
Viroid Infection Process and Management
Virusoids (Encapsidated, Viroidlike, Satellite RNAs)
107
107
108
109
Chapter 10. Viruses

Structure of Viruses
Mechanism of Plant Virus Infection
Inclusion Bodies
Satellite Viruses, Satellite RNA, and Defective Interfering
Viruses
Classification of Viruses
Systematic Position of Important Viral Pathogens
Symptoms of Crop Virus Diseases
Mode of Transmission of Virus Diseases
Virus Disease Diagnosis
Important Crop Diseases Caused by Viruses
111
111
118
122
123
124
130
140
141
152
152
COMMON DISEASE SYMPTOMS, ASSESSMENT,

AND DIAGNOSIS
Chapter 11. Common Disease Symptoms
157
Chapter 12. Crop Disease Assessment

Disease Incidence Assessment
Disease Severity Assessment
Disease Index
Area Under Disease Progress Curve (AUDPC)
Remote Sensing
Digital Image Analysis
165
165
166
169
169
169
170
Chapter 13. Crop Disease Diagnosis

Diagnosis Based on Disease Symptoms
Diagnosis by Immunological Techniques
Nucleic Acid Probe-Based Methods
Diagnosis of Crop Fungal Diseases
Diagnosis of Crop Bacterial Diseases
Diagnosis of Crop Viral Diseases
Diagnosis of Viroid Diseases
Diagnosis of Phytoplasma Diseases
Plant Clinics and Diagnostic Service Laboratories
Digitally Assisted Diagnosis
173
173
174
184
190
191
192
195
195
196
196
CROP DISEASE EPIDEMIOLOGY

Chapter 14. Assessment of Disease Progress
What Is Epidemiology?
Disease Progress Curve
Area Under Disease Progress Curve (AUDPC)
Apparent Infection Rate
205
205
206
208
209
Chapter 15. Factors Involved in Disease Increase

Environmental Factors Affecting Sporulation
Environmental Factors Affecting Spore Release
Environmental Factors Affecting Spore Dispersal
Measurement of Spore Dispersal
Disease Gradients
211
211
212
214
215
218
Environmental Factors Affecting Spread of Bacterial

Pathogens
Environmental Factors Affecting Infection and Disease
Development
Chapter 16. Forecasting Models
Types of Models
Specific Models
219
219
225
225
226
CROP DISEASE MANAGEMENT

Chapter 17. Biological ControlMicrobial Pesticides
Trichoderma
Pseudomonas Species
Other Organisms
Commercially Available Microbial Pesticides
239
239
245
251
258
Chapter 18. Biological ControlMycorrhiza

What Is a Mycorrhiza?
Diseases Controlled by Mycorrhizal Fungi
Mechanisms Involved in Biocontrol by Mycorrhizae
Mass Production of Mycorrhizal Fungi
Commercialization of Mycorrhizae
271
271
272
272
273
274
Chapter 19. Breeding for Disease Resistance

Conventional Breeding
Types of Disease Resistance
Interaction Between Resistance Genes
Molecular Marker-Assisted Selection
Near-Isogenic Lines (NIL)
Common Breeding Methods for Disease Resistance
Breeding for Quantitative (Partial) Resistance Using QTL
Linked with Markers
Chapter 20. Breeding for Disease Resistance
Genetic Engineering
Transgenic Plants Expressing Plant Resistance Genes
Transgenic Plants Expressing Plant Defense Genes
Transgenic Plants Expressing Genes Involved in a Signal
Transduction System
277
277
279
280
283
283
285
293
293
295
299
Transgenic Plants Expressing Plant Proteinase-Inhibitor

Genes
Transgenic Plants Expressing Viral Genes
Transgenic Plants Expressing Fungal and Bacterial Genes
Transgenic Plants Expressing Insect Genes
Transgenic Plants Expressing Animal Genes
Transgenic Plants Expressing Detoxification Genes
Transgenic Plants Expressing Genes Encoding Antibodies
Practical Utility of Transgenic Plants in Crop-Disease
Management
Chapter 21. Breeding for Disease ResistanceIn Vitro
Selection
What Is In Vitro Selection?
Types of In Vitro Selection
Stability and Heritability of Resistance Evolved Through
In Vitro Selection
302
302
306
308
310
311
312
312
325
325
326
329
Chapter 22. Chemical ControlBacterial Diseases

Common and Trade Names of the Bactericides
and Their Uses
Effectiveness of Bactericides
333
333
336
Chapter 23. Chemical ControlFungal Diseases

History of Chemical Control
Development of Commercially Viable Fungicides
Ingredients in Fungicide Formulations
Types of Fungicide Formulations
Classification of Fungicides
Mode of Action of Fungicides
Spectrum of Diseases Controlled by Individual Fungicides
Fungicide Application
Fungicide Resistance
337
337
339
353
357
367
371
381
395
398
Chapter 24. Chemical ControlFungicide Application

Equipment
Dusters
Sprayers
Granule Applicators
Seed Treaters
Soil Application Equipment
403
403
404
407
407
408
Other Types of Equipment

Parts of Application Equipment
408
409
Chapter 25. Chemical ControlVirus Diseases
413
Control of Virus Spread by Viricides

Control of Virus Spread by Insecticides
Control of Virus Diseases by Plant Activators
413
413
415
Chapter 26. Cultural Methods
417
Prevention of Introduction of Inoculum

Soil Solarization
Organic Amendments
Mulching
Living Ground Covers
Catch Crops
Tillage
Spacing
Manipulation of Soil pH
Inorganic Amendments
Crop Rotation and Intercropping
Irrigation
Hydrophobic Particle Films
417
419
419
422
422
423
423
423
424
424
425
426
426
Chapter 27. Exclusion and Eradication
431
Exclusion
Eradication
List of Important Seedborne Pathogens
Seed Health Testing and Certification
Seed Health Testing Methods
Indexing Plant Propagation Materials
431
433
434
436
437
442
Chapter 28. Plant Activators

What Are Plant Activators?
Types of Plant Activators
Practical Uses of Plant Activators
Chapter 29. Plant Extracts
Antimicrobial Action of Plant Extracts
Induction of Disease Resistance by Plant Extracts
445
445
446
455
461
461
462
CROP DISEASES
Chapter 30. Diseases of Major Cultivated Crops
Apple (Malus domestica Borkh.)
Banana (Musa species)
Barley (Hordeum vulgare L. emend. Bowden)
Bean (Phaseolus vulgaris L.)
Citrus (Citrus species)
Corn (Zea mays L.)
Cotton (Gossypium species)
Crucifers
Cucurbits
Grape (Vitis species)
Oats (Avena sativa L.)
Pea (Pisum sativum L.)
Peach [Prunus persica (L.) Batsch]
Peanut (Arachis hypogaea L.)
Pear (Pyrus communis L.)
Plum (Prunus domestica L.)
Potato (Solanum tuberosum L.)
Rice (Oryza sativa L.)
Tomato (Lycopersicon esculentum Mill.)
Wheat (Triticum spp.)
467
467
470
472
475
477
478
482
483
485
487
490
492
492
494
497
498
499
501
502
505
HOST DEFENSE MECHANISMS

Chapter 31. Cell Wall Reinforcement
Cell Wall Modifications
Role of Cell Wall Modifications in Disease Resistance
511
511
512
Chapter 32. Hydroxyproline-Rich Glycoproteins

Types of Cell Wall Proteins
Role of HRGPs in Disease Resistance
515
515
515
Chapter 33. Lignin

Biosynthesis of Lignin
Role of Lignin in Disease Resistance
519
519
520
Chapter 34. Pathogenesis-Related Proteins

Definition
Classification of PR Proteins
Role of PR Proteins in Disease Resistance
523
523
525
530
Chapter 35. Phenolics

Soluble Phenolics and Disease Resistance
Wall-Bound Phenolics and Disease Resistance
537
537
539
Chapter 36. Phytoalexins

Definition
Antimicrobial Action of Phytoalexins
Role of Phytoalexins in Disease Resistance
543
543
544
545
PATHOGENESIS
Chapter 37. Physiology of Pathogenesis
Adhesion
Nutrition
Plant Barriers Against Pathogens
Enzymes
Toxins
Role of Toxins in Pathogenesis
551
551
552
554
554
559
562
Glossary
569
Index
587
ABOUT THE AUTHOR
P. Vidhyasekaran, PhD, FNA, is the former Director of the Center for

Plant Protection Studies, Tamil Nadu Agricultural University, Coimbatore,
India. He is the author of eight books from leading publishers in the United
States and India. He has published more than 400 articles in 32 international
journals as well as several book chapters. Dr. Vidhyasekaran has served on
the editorial boards of several journals and has been elected President of the
Indian Society of Plant Pathologists. He is a Fellow of the National Academy of Agricultural Sciences and several other scientific societies, and a
member of the New York Academy of Sciences. Dr. Vidhyasekaran has won
several national awards as well as the Tamil Nadu Best Scientist Award. He
has served as a visiting scientist in 14 countries, including the United States,
Philippines, and Denmark. He is the author of Bacterial Disease Resistance
in Plants: Molecular Biology and Biotechnological Applications (Haworth).
Foreword
Plant diseases cause billions of dollars in crop losses each year throughout the world. Historically, uncontrolled plant disease epidemics have resulted in great human suffering through famine and resultant social upheaval. Perhaps the best-known example is the Irish famine of 1846, in
which two million people died and many more emigrated from their homeland. The famine was caused by the destruction of the potato crop, the staple
food in Ireland at that time, by Phytophthora infestans, the causal agent of
late blight disease. The efforts of early scientists such as De Bary, Speerschneider, and Khn to find the causes of late blight and other plant diseases
established plant pathology as a critical discipline in the struggle to prevent
catastrophic crop failure.
The science of plant pathology seeks to understand and work to manage
such devastating outbreaks of plant diseases. It describes (1) what causes
disease, (2) how pathogens interact with plants to cause disease, (3) how
diseases spread and develop into epidemics, (4) the scope of losses caused
by disease, and (5) disease management. It is a very broad field, incorporating plant anatomy, physiology, breeding and genetics, virology, bacteriology, mycology, nematology, molecular biology, biochemistry, mathematics, computer modeling, and statistics. It is a daunting task indeed to pull all
of this together in one book.
Professor Vidhyasekaran has written an encyclopedia that describes the
breadth of subjects in the field of plant pathology. It will serve as a beginning
reference for students and professionals in plant pathology as well as allied
fields. Each section provides an overview of a given area, which will serve as
both an introduction and a starting point for further studies. It also comprehensively describes modern plant pathology. It includes classification of pathogens based on DNA analyses and protein patterns, molecular diagnostic techniques, computer-aided decision support systems, remote sensing, digital image
analysis, microbial pesticides, plant activators, molecular marker-assisted
breeding, gene pyramiding, genetic engineering, in vitro breeding, and molecular plant pathology. Professor Vidhyasekaran and The Haworth Press are
to be commended for providing this excellent resource.
Sally A. Miller
Professor, Department of Plant Pathology
The Ohio State University
Ohio Agricultural Research and Development Center
Preface
Preface
Globally, diseases cause heavy crop losses amounting to several billion

dollars annually. Some diseases have shattered the economies of nations.
Late blight of potato is known to be responsible for the Irish famine of 18451847 when about two million people died because of starvation in Ireland.
Canker is the most serious disease in Citrus spp. and is widely prevalent in
Florida, Georgia, Alabama, Louisiana, South Carolina, Texas, and Mississippi. Eradication programs have been initiated by several states since 1915.
By 1991, more than 20 million citrus trees were destroyed at a cost of about
$94 million. Still the Asiatic citrus canker is prevalent in several parts of the
United States. Disease management is still a challenging task, and plant pathology is the most important subject in crop science. This encyclopedia
covers the entire field of plant pathology. It defines the numerous subjects
covered as well as describes them. It is designed to stand as a compendium
of current knowledge about the topics in plant pathology. This encyclopedia
will serve as a single, easy-to-use reference work including a full range of
subject areas associated with plant pathology. Each section in this encyclopedia serves as a comprehensive overview of a given area, providing both
breadth of coverage for students and depth of coverage for research professionals.
This book provides the latest nomenclature and classification of bacterial, fungal, viral, and phytoplasma pathogens and the systematic position of
each crop pathogen (more than 1,500 pathogens). It describes disease and
crop loss assessment models, remote sensing, digital image analysis, disease forecasting models, computer-aided decision support systems, and
plant clinics. The book consists of detailed sections on microbial pesticides,
induced systemic resistance, mycorrhiza, seed health testing, indexing
plant-propagation materials, a complete list of fungicides, ready-formulated
mixtures of fungicides, fungicide resistance, modern fungicide application
equipments, bactericides, and viricides. This comprehensive book on modern plant pathology describes classification of pathogens based on DNA
analyses and protein profiles, molecular diagnostic techniques, commercial
development of microbial pesticides, plant products and plant activators,
and novel breeding techniques using molecular marker-assisted selection
and gene pyramiding. This book also describes molecular biology of plantpathogen interactions and host defense mechanisms and in vitro selection
and genetic engineering technologies. It also provides a list of plant pathology terminology. It is unique in providing a complete list of diseases of all
major crops cultivated in tropics, semitropics, and temperate regions of the
world and the accepted names, synonyms, and anamorphic and teleomorphic names of pathogens (over 910 fungal, 315 viral, and 200 bacterial
pathogens) with authority. It is intended for a broad international audience
of graduate and undergraduate students, university faculty, public and private sector research scientists, extension specialists, and development
workers engaged in plant protection. This book will be an indispensable resource for plant pathologists, mycologists, bacteriologists, virologists, botanists, and graduate-level students in these disciplines.
Birth and
BirthDevelopment
and Development ofof
Plant
Plant
Pathology
Pathology
Plant pathology is the science or study of plant diseases. The word disease means any impairment of normal physiological function of plants,
producing characteristic symptoms. A symptom is a phenomenon accompanying something and is regarded as evidence of its existence. Disease is
caused by pathogen. A pathogen is any agent that can cause disease. Plant
pathology describes: (1) what causes disease, (2) how the disease is caused,
(3) how the disease spreads, causing epidemic, (4) how much loss the disease can cause, and (5) how to manage the disease.
HISTORY
Ancient History
Occurrence of diseases in plants has been recognized from ancient times.
Around 2000 B.C., from the Babylonian kingdoms, a disease called samana
in barley was mentioned in a farm almanac. Books of the Old Testament that
date to the eighth and fifth centuries B.C. contain references to the blasting
and mildew of crops as great scourges of humankind. In the third century
B.C., several Greek writings began to contain references to plant diseases. A
Greek named Cleidemus is often identified as the first plant pathologist and
grandfather of plant pathology according to McNew (1963). He made observations on diseases of grapes, figs, and olives. The Greek philosopher
Theophrastus (c. 372-c. 287 B.C.) was the first to study and write about diseases of trees, cereals, and legumes. He is known as the father of botany.
A Roman author, Marcus Terentius Varro (116-27 B.C.) in his Rerum
Rusticarum mentions the god Robigus among the deities to be propitiated.
According to Varro, Robigus is the rust god, who had to be depended upon
to protect cereal crops from rust attack. Propitiatory ceremonies, the Robigalia, were performed in April or May each year when rust often first became noticeable. Other gods such as Flora, Ceres (who protected grain
crops), Bacchus (grapes), and Minerva (olives) also had to be propitiated. In
his writing Historia Naturalis, Pliny the Elder (23-79 A.D.) described many
control measures against diseases. He mentioned treating cereal seeds with

wine or a concoction of cypress leaf extract to control mildew. Palladius
(around fourth to fifth centuries) wrote about plant diseases in his De Re
Rustica. He described differences in the susceptibility of different cultivars
to diseases and suggested sanitation through removal of diseased plants.
A German, Gottfried von Franken (circa 1200 A.D.), in his Pelzbuch, suggested control measures against cankers of cherry and other fruit diseases. A
chapter on grapevine diseases is found in the book titled Ruralium Commodorum Libri XII written by Petrus Crescentius (1230-1320) during the
thirteenth century. Kreuterbuch by German writer Jerome Bock (14981554) contains an illustration of wheat plants affected by loose smut. Robert
Hooke (1635-1703) in England developed a microscope and described teliospores of Phragmidium mucronatum on rose in his book Micrographia,
which was written in 1665. In 1679, Marcello Malpighi (1628-1694) in his
Anatome Plantarum, described aecia of Gymnosporangium on hawthorn. In
1675, Dutch naturalist Antoni van Leeuwenhoek (1632-1723) discovered
bacteria while working with a microscope he had built.
Eighteenth Century
In 1705 Joseph Pitton de Tournefort (1656-1708), a French botanist, reported in a journal article that fungi could reproduce by bodies similar to
seeds and could incite disease in plants under humid conditions in the greenhouse. In 1727, Stephen Hales (1677-1761) from England published on
powdery mildew of hop. Henri Louis Duhamel du Monceau (1700-1782), in
France described Helicobasidium purpureum in 1728. The Italian Micheli
(1679-1737) published his book Nova Plantarum Genera in 1729 and introduced the generic name Puccinia in his book. In 1753, Swedish botanist Carolus Linnaeus (1707-1778) developed the binomial system of nomenclature in botany and published Species Plantarum. He included ten genera of
fungi in his book.
In 1755, French scientist Mathieu Tillet (1714-1791) added black dust
from bunted wheat to seed from healthy wheat and observed that bunt was
more prevalent in plants produced from such seed than from nondusted
seed. He showed the efficacy of seed treatment in the control of wheat bunt,
and he recommended washing seeds in lye, drying, sprinkling with lye, and
dusting with lime. This is the first seed treatment recommended to control
diseases. Fontana (1730-1805) from Italy described teliospores and uredospores of wheat rust in 1767. In the same year, Tozzetti (1712-1783) from Italy described rust diseases. In 1774, Danish entomologist Johann Christian
Fabricius (1745-1808) published a system of classification of fungal pathogens in which he arranged pathogens by classes, genera, and species.
Nineteenth Century
In 1801, Christian Hendrik Persoon (1761-1836) from France published
his book Synopsis Methodica Fungorum; this was the first reliable systematic account of fungi. Benedict Prevost (1755-1819) in 1807 gave the first
experimental proof that a fungus could cause disease in a plant. He recommended copper sulfate as seed treatment to control bunt disease in wheat. In
1817, Thomas Andrew Knight (1759-1838) reported that sulfur controlled
scab (Venturia pirina) in pear trees. In 1821 Elias Magnus Fries (17941878) published Systema Mycologicum providing classification of fungi.
John Robertson (1824) from Ireland showed that mildew (Sphaerotheca
pannosa var. persicae) is controlled by a mixture of sulfur in soapsuds. Control of peach leaf curl (Taphrina deformans) with lime and sulfur was demonstrated by Knight in 1842. Miles Joseph Berkeley (1803-1889) in 1845
published papers on diseases of cereals and vegetables. In 1853, Heinrich
Anton De Bary (1831-1888) worked on smut and rust fungi, downy mildews, and late blight of potato. He discovered two alternate hosts for the
rusts. Speerschneider (1857) proved that Phytopthora infestans was the cause
of potato late blight. Julius Gotthelf Khn (1825-1910) published the first
textbook of plant pathology in 1858.
Thomas Jonathan Burrill (1839-1916) was the first person to show that
bacteria can cause plant diseases, and in 1878 he described fire blight of
pear and other fruits. In 1884, Robert Koch (1843-1910) developed his postulates, which stipulate conditions for describing an organism as the cause
of a disease. In 1885, Alexis Millardet (1838-1902) developed Bordeaux
mixture as a fungicide. In 1886, Adolph Mayer (1843-1942) worked on mosaic of tobacco and called it mosaic disease of tobacco. He showed that the
causal agent was transmissible to healthy plants in juice extract. Smith
(1891) was working on peach yellows disease and reported that the causal
agent was contagious and was bud transmitted. In 1891, Joseph Charles Arthur (1850-1942) identified wheat varieties resistant to scab disease. In
1892, Russian scientist Dmitrii Iosifovich Ivanowski (1864-1924) showed
that tobacco mosaic causal agent is filterable through bacteriological filters.
In 1898, Dutch scientist Martinus Willem Beijerinck (1851-1931) called the
causal agent contagium vivum fluidum. He used the word virus to describe the contagium. He demonstrated that it was graft transmissible. Jakob
Eriksson (1848-1931) discovered the physiologic races in rust pathogens in
1896.
MODERN PLANT PATHOLOGY

During the twentieth century, plant pathology developed as a full-fledged
science. Biffin (1905) bred the first resistant host cultivar using Mendelian
principles. Allard (1914) partially purified tobacco mosaic virus (TMV).
Antigenic nature of plant viruses was discovered by Dvorak in 1928. In
1928, Purdy developed antiserum against viruses, according to Corbett
(1964). Holmes (1929) developed the TMV local lesion assay. Wendell
Meredith Stanley (1904-1971) obtained crystals of TMV in 1935 and received a Nobel Prize for crystallizing TMV in 1946. Ernest Athearn Bessey
(1877-1957) authored the first American textbook of mycology, which was
published in 1936.
During the first 50 years of the century, most of the contributions in the
field of mycology were by traditional and taxonomically inclined mycologists. By midcentury, the approach had shifted from a traditional taxonomic
focus to one that stressed the biology of fungi (Sequeira, 2000). Fungal
physiology became a subject of great interest and host-parasite interactions
were studied in detail (Keen, 2000). In 1910, Lewis Ralph Jones (18641945) suggested the role of pectic enzymes in plant disease development.
Subsequently, the role of enzymes and toxins in disease development has
been demonstrated. Several toxins were isolated and purified in the 1950s.
Meehan and Murphy (1947) identified a host-specific toxin from Helminthosporium victoriae. Since then, several host-specific toxins have been
identified from various fungal pathogens.
In 1942, Harold Flor developed the gene-for-gene concept. Flor hypothesized that the genes which condition the reaction of the host could be identified only by their interaction with specific strains of the parasite, while those
which condition pathogenicity in the parasite could be identified only by
their interaction with specific varieties of the host (Flor, 1942). Several disease-resistant genes have been identified. Newer techniques to develop durable resistance have been initiated. The major breakthrough in this field is
successful cloning of resistance genes. Johal and Briggs (1992) cloned the
first disease-resistant gene in 1992. Molecular breeding for disease resistance is the modern method of disease management. In 1998, Williamson
and his associates demonstrated that a single cloned disease-resistant gene,
Mi from tomato, confers resistance against a nematode and an insect (Rossi
et al., 1998).
Van der Plank (1963) published a book titled Plant DiseasesEpidemics
and Control; his work facilitated the rapid development of the field of epidemiology. Several disease-forecasting modules have been developed (Jones,
1998). Rapid advances have been made in the field of virology. The presence of satellite viruses was first reported by Kassanis in 1962. In 1968,
Shepherd and his associates discovered that cauliflower mosaic virus is a
DNA virus (Shepherd et al., 1968). Satellite virus is dependent on its virus
for its replication. Satellite RNAs were first reported by Schneider in 1969.
In 1971, Diener described viroids. Several molecular diagnostic techniques
have been developed to detect plant diseases. Plant viral genomes have been
cloned (Boyer and Haenni, 1994). Transgenic plants expressing capsid protein of a virus were first developed in 1986 by Beachey and his co-workers
(Abel et al., 1986). Transgenic plants expressing satellite RNAs, viral replicase genes, and defective movement protein genes have all been developed
(Zaitlin and Palukaitis, 2000).
Molecular plant pathology has emerged as an attractive field in the latter
part of the century. In 1940, Muller and Borger proposed the phytoalexin
theory. Several signal molecules have been identified from pathogens and
host. Albersheim and his associates identified a highly active elicitor from a
fungal cell wall in 1984 (Sharp et al., 1984). Elicitor molecules, which are
the products of avirulence genes, have been characterized. The first avirulence gene was cloned from Pseudomonas syringae pv. glycinea by Staskawicz et al. in 1984. Several kinds of pathogenesis-related proteins have
been identified. Genes encoding them have been cloned, and transgenic
plants expressing them have been developed. In 1991, Broglie and colleagues developed transgenic plants expressing the PR-3 gene. More than
100 fungicides have been developed during the twentieth century. The development of systemic fungicides with activity against broad spectrum of
fungal pathogens was a major breakthrough in the history of plant pathology. Plant (defense) activators with simultaneous action against fungal, bacterial, and viral diseases have been identified. Biocontrol agents, which are
capable of inducing systemic resistance, have been developed as commercial products and their efficacy in control of a wide range of diseases in
commercial farms has been demonstrated (Vidhyasekaran, 2001). All these
facets of modern plant pathology are described in detail in this book.
Plant pathology is now developing very rapidly. Plant pathologists are
using many modern tools such as gene cloning, genetic engineering, molecular diagnostics, molecular-assisted breeding, remote sensing, digital imaging, and molecular manipulation of signaling systems to develop both plants
with built-in resistance to pathogens and sustainable integrated disease
management systems in various cropping systems. This book describes all
of these novel approaches in the field of modern plant pathology.
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Biffin, R. H. (1905). Mendels laws of inheritance and wheat breeding. J Agric Sci,
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Boyer, J. C. and Haenni, A. L. (1994). Infectious transcripts and cDNA clones of
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Broglie, K., Chet, I., Holliday, M., Cressman, R., Biddle, P., Knowlton, S., Mauvais, C. J., and Broglie, R. (1991). Transgenic plants with enhanced resistance to
the fungal pathogen Rhizoctonia solani. Science, 254:1194-1197.
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Diener, T. O. (1971). Potato spindle tuber virus. IV. A replicating, low molecular
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Holmes, F. O. (1929). Local lesions in tobacco mosaic. Bot Gaz, 87:39-55.
Johal, G. S. and Briggs, S. P. (1992). Reductase activity encoded by the HM1 disease resistance gene in maize. Science, 258:985-987.
Jones, D. G. (1998). The Epidemiology of Plant Diseases. Kluwer Academic Publishers, Dordrecht, pp. 460.
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Keen, N. T. (2000). A century of plant pathology: A retrospective view on understanding host-parasite interactions. Annu Rev Phytopathol, 38:31-48.
McNew, G. L. (1963). The ever-expanding concepts behind 75 years of plant pathology. In S. Rich (Ed.), Perspectives of Biochemical Plant Pathology. Conn.
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Meehan, F. and Murphy, H. C. (1947). Differential phytotoxicity of metabolic byproducts of Helminthosporium victoriae. Science, 106:270-271.
Muller, K. O. and Borger, H. (1940). Experimentelle Untersuchungen uber die Phytophthora-Resistenz der Kartoffel. Zugleich ein Beitrag zum Problem der erworbenen Resistenz in Pflanzenreich. Arb Biol Reichsanst Land Forstwirtsch,
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Robertson, J. (1824). On the mildew and some other diseases incident to fruit trees.
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Schneider, I. R. (1969). Satellite-like particle of tobacco ringspot virus that resembles tobacco ringspot virus. Science, 166:1627-1629.
Sequeira, L. (2000). Legacy for the millennium: A century of progress in plant pathology. Annu Rev Phytopathol, 38:1-7.
Sharp, J. K., McNeil, M., and Albersheim, P. (1984). The primary structure of one
elicitor-active and seven elicitor-inactive hexa ( -D-glucopyranosyl)D-glucitols
isolated from the mycelial walls of Phytophthora megasperma f. sp. glycinea.
J Biol Chem, 259:11321-11336.
Shepherd, R. J., Wakeman, R. J., and Romanko, R. R. (1968). DNA in cauliflower
mosaic virus. Virology, 36:150-152.
Smith, E. F. (1891). Additional evidence on the communicability of peach yellows
and peach rosette. US Dep Agric Div Veg Pathol Bull, 1:1-65.
Speerschneider, J. (1857). Die Ursache der Erkrankung der Kartoffelknolle durch
ein Reihe Experimente beweisen. Bot Zeit, 14:121-125.
Staskawicz, B. J., Dahlbeck, D., and Keen, N. T. (1984). Cloned avirulence gene of
Pseudomonas syringae pv. glycinea determines race-specific incompatibility on
Glycine max (L.). Merr. Proc Natl Acad Sci USA, 81:6024-6028.
Van der Plank, P. E. (1963). Plant Diseases: Epidemics and Control. Academic
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Vidhyasekaran, P. (2001). Induced systemic resistance for the management of rice
fungal diseases. In S. Sreenivasaprasad and R. Johnson (Eds.), Major Fungal
Diseases of Rice: Recent Advances. Kluwer Academic Publishers, the Netherlands, pp. 347-358.
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virus diseases. Annu Rev Phytopathol, 38:117-143.
CAUSAL ORGANISMS
Bacteria
Bacteria
STRUCTURE OF BACTERIAL CELLS
Bacteria are primitive organisms classified as prokaryotes, with a primitive type of nucleus lacking a clearly defined membrane. Most of the genetic
information in a bacterial cell is carried on a single chromosome with double-stranded deoxyribonucleic acid (DNA) in a closed circular form. A
chromosome is the carrier of genes (the factors that control inherited traits).
Genes are deoxyribonucleic acids that are linked and aligned on the chromosome. Chromosomes are located in the chromatin body of the bacteria.
In addition, some bacterial cells contain extrachromosomal DNA as plasmids. Plasmids are circular DNA molecules that are generally dispensable
and not essential for cell growth and division. However, they confer traits
such as antibiotic resistance or pathogenicity on the host organism. Plasmids replicate independently of the chromosomes and can pass from one
bacterial cell to another with ease. Some bacteria contain episomes, which
are also autonomous and dispensable genetic elements similar to plasmids.
Unlike plasmids, however, episomes can exist even as integrated with the
chromosome. Generally, the bacteria containing plasmids do not have
episomes and vice versa. Transposons, mobile DNA segments that can insert into a few or several sites in a genome, are found in some bacteria.
Transposons are transposable genetic elements (the word transpose means
alter the positions of or interchange) that are capable of moving between prokaryotes and eukaryotes.
Bacterial cells have either no organelles or poorly developed organelles.
All bacteria except Streptomyces are unicellular. Bacteria are rod shaped,
round (spherical, ovoid, or ellipsoidal), or spiral (helices). All plant-pathogenic bacteria are rod shaped. They measure 0.5-3.5 m in length and 0.31.0 m in diameter. Each bacterial cell consists of a cell wall and a compound membrane called the cytoplasmic membrane, which encloses the
cells protoplasm. Outside the cell wall is a slime layer. The slimy material
may remain firmly adhered as a discrete covering layer of each cell, or it
may part freely from the cell as it is formed. The former, a thick, well-devel-
oped slime layer, is called a capsule, while the latter is called free slime.
Most plant-pathogenic bacteria have one or more flagella. Flagella may occur at one or both ends (polar flagella) or all over the surface of the bacterium (peritrichous flagella). Flagella may allow some movement of the bacteria.
The bacterial cell wall is composed of a peptidoglycan. The peptidoglycan is also called a mucopeptide or murein. Murein is composed of six
different components such as N-acetylglycosamine, N-acetylmuramic acid,
L-alanine, D-alanine, D-glutamic acid, and either L-lysine or meso-diaminopimelic acid. The rigid peptidoglycan layer is located between the cytoplasmic membrane and a multiple-tract layer. The multiple-tract layer is
composed of lipoprotein and lipopolysaccharide complexes. The external
multiple-tract layer, the rigid peptidoglycan layer, and the cytoplasmic
membrane constitute the bacterial envelope.
Immediately below the cell wall is the cytoplasmic membrane. The
membrane contains about 75 percent protein, 20 to 30 percent lipid, and
about 2 percent carbohydrate. The membrane is semipermeable and controls the passage of nutrients and metabolites into and out of the cell. Besides the cytoplasmic membrane, many bacteria possess other intracellular
membrane systems such as mesosomes or chondrioids. The mesosome
structure is formed by an invagination of the cytoplasmic membrane. Mesosomes serve for compartmentalization and integration of biochemical systems.
Cytoplasm is the cell material within the cytoplasmic membrane. The cytoplasm can be divided into the area rich in ribonucleic acid (RNA), the
chromatinic area (nuclear area) rich in DNA, and the fluid portion with dissolved nutrients. Bacteria do not have a characteristic nucleus. They contain
bodies within the cytoplasm that are regarded as a nuclear structure and
DNA is confined to this area. Because it is not a discrete nucleus, it is called
a chromatin body.
Ribosomes are globular structures found in the cytoplasm. They are composed of about one-third protein and two-thirds RNA. Ribosomes are designated 30S, 50S, 70S, etc., depending upon their size. The size is determined
by the rate, measured in Svedberg units, at which a particle sediments when
it is centrifuged at high speed in an ultracentrifuge. Ribosomes that act in
clusters are called polyribosomes or polysomes. Polysomes may contain
three to 70 ribosomes depending upon the bacteria. Ribosomes are the sites
of protein synthesis.
Vacuoles are cavities in the cytoplasm that contain a fluid called cell sap.
As the cell approaches maturity, some of the water-soluble reserve food materials manufactured by the cell are dissolved in the sap. Insoluble constituents precipitate out as cytoplasmic inclusion bodies. Volutin, glycogen, and
fat globules are common cytoplasmic inclusions. Volutins are metachromatic granules. They are found to localize in the vacuoles of mature forms.
They contain inorganic polyphosphate, lipoprotein, RNA, and magnesium.
They may serve as phosphate storage structures. Glycogen accumulates in
the cytoplasm at the ends of the cell in the form of granules. Lipids are found
in the cytoplasm of bacteria in the form of fat globules.
Fimbriae and pili are hairlike structures that are attached to the bacterial
cells as appendages. Fimbriae are common in plant-pathogenic bacteria.
Pili are considered to be sex organs. They mediate conjugation (mating) of
bacteria. They may also serve as adsorption organs for bacteriophages.
Actinomycetes are now classified as a group of bacteria. They produce
individual, small, bacteria-like spores and funguslike vegetative mycelium.
REPRODUCTION OF BACTERIA
The predominant mode of reproduction in bacteria is binary fission. The
bacterial cell divides into two daughter cells. This is an asexual process. A
transverse wall develops across the middle of the bacterial cell. When new
cell wall material has developed, the cells separate. During this process, the
DNA condenses into an amorphous mass, which elongates and becomes
dumbbell shaped before it divides into two equal pieces. These pieces serve
as the nuclei of the daughter cells. This process is repeated every 20 minutes
and the bacteria multiply in logarithmic proportion. However, the multiplication of the bacteria is limited by the exhaustion of available nutrients
and/or the accumulation of toxic metabolic products.
Sometimes, bacteria may reproduce sexually by conjugation. Genetic
material of one cell is transferred to another cell during conjugation. The
two cells are genetically different. The donor cell transfers part of its genome (set of genes) to the recipient cell. Donor strains are designated F+ or
Hfr (males, high frequency cells). The recipient strains are designated F(females). The F factor is called the sex factor. It is a type of episome.
Episomes are genetic elements that can exist in two alternative states: autonomous in the cytoplasm or incorporated in the chromosome. In the former
condition, the episome is free to multiply independently of cell division. In
the latter, it is replicated only when the chromosome is replicated. F factor
controls the ability of the cells to act as gene donors. In the process of conjugation, the donor cell injects a chromosomal thread into the recipient cells,
and the genes along this thread enter in a definite order, one after another, as
they occur in a particular donor strain. Conjugation is one type of genetic recombination. Genetic recombination refers to any process leading to the
formation of a new individual that derives some of its genes from one parent
and some from another, genetically different, parent. The recipient cell,
which receives genes from a donor, is called a recombinant.
NOMENCLATURE OF BACTERIAL PATHOGENS
The nomenclature of plant bacterial pathogens has been revised in recent
years according to the International Code of Nomenclature of Bacteria
(ICSB, 1992). A list of all valid names of plant-pathogenic bacteria from
1864 to 1995 was published by Young et al. (1996). More than 330 bacterial
pathogens were listed. According to Schaad et al. (2000), the names of some
of these bacterial pathogens still need to be modified. Accepted names
(Young et al., 1996) of some important bacterial pathogens are provided
here. Names suggested by Schaad et al. (2000) are given within parentheses
and marked with an asterisk (*).
Apple fire blight: Erwinia amylovora (Burrill 1882) Winslow et al. 1920
Banana moko wilt: Ralstonia solanacearum (Smith 1896) Yabauuchi,
Kosako, Yano, Hotta, and Nishiuchi 1995
Bean bacterial brown spot: Pseudomonas syringae pv. syringae van Hall
1902
Bean common blight: Xanthomonas axonopodis pv. phaseoli (Smith
1897) Vauterin, Hoste, Kersters, and Swings 1995 (X. campestris pv.
phaseoli [Smith 1897] Dye 1978)*
Bean halo blight: Pseudomonas savastanoi pv. phaseolicola (Burkholder
1926) Gardan, Bollet, Abu Ghorrah, Grimont, and Grimont 1992 (P.
syringae pv. phaseolicola [Burkholder 1926] Young, Dye, and Wilkie
1978)*
Cabbage and cauliflower black rot: Xanthomonas campestris pv.
campestris (Pammel 1985) Dowson 1939
Citrus canker: Xanthomonas axonopodis pv. citri (Hasse 1915) Vauterin,
Hoste, Kersters, and Swings 1995 (X. campestris pv. citri [Hasse 1915]
Dye 1978)*
Citrus greening: Citrus greening organism (Nonculturable, phloemrestricted, Gram-negative bacteria) Candidatus Liberobacter africanum
Jagoueix et al. 1994 and Candidatus Liberobacter asiaticum Jagoueix
et al. 1994
Citrus stubborn: Spiroplasma citri Saglio, Lhospital, Lafleche, Dupont,
Bove, Mouches, Rose, Coan, and Clark 1986
Citrus variegated chlorosis: Xylella fastidiosa Wells, Raju, Weisburg,
Mandelo-Paul, and Brenner 1987
Corn Stewarts disease (bacterial wilt): Pantoea stewartii (Smith 1898)

Mergaert et al.,1993
Cotton bacterial blight (black arm): Xanthomonas axonopodis pv.
malvacearum (Smith 1901) Vauterin, Hoste, Kersters, and Swings
1995 (X. campestris pv. malvacearum [Smith 1901] Dye 1978)*
Peach bacterial canker: Pseudomonas syringae pv. syringae van Hall
1902
Peach bacterial spot: Xanthomonas arboricola pv. pruni (Smith 1903)
Vauterin, Hoste, Kersters, and Swings 1995
Pear fire blight: Erwinia amylovora (Burrill 1882) Winslow, Broadhurst,
Buchanan, Krumwiede, Rogers, and Smith 1920
Plum bacterial canker: Pseudomonas syringae pv. syringae van Hall 1902
Plum bacterial spot: Xanthomonas arboricola pv. pruni (Smith 1903)
Potato bacterial wilt (brown rot): Ralstonia solanacearum (Smith 1896)
Yabauuchi, Kosako, Yano, Hotta, and Nishiuchi 1995
Potato blackleg and bacterial soft rot: Erwinia carotovora ssp. atroseptica
(van Hall 1902) Dye 1969, E. carotovora ssp. carotovora (Jones 1901)
Bergey, Harrison, Breed, Hammer, and Huntoon 1923, and E.
chrysanthemi Burkholder, McFadden, and Dimock 1953
Potato common scab: Streptomyces scabies (ex Thaxter 1892) Lambert
and Loria 1989
Potato ring rot: Clavibacter michiganensis ssp. sepedonicus
(Spieckermann and Kotthoff 1914) Davis, Gillaspie, Vidaver, and Harris 1984
Rice bacterial blight: Xanthomonas oryzae pv. oryzae (Ishiyama 1922)
Swings, Van den Mooter, Vauterin, Hoste, Gillis, Mew, and Kersters
1990
Rice bacterial leaf streak: Xanthomonas oryzae pv. oryzicola (Ishiyama
1922) Swings, Van den Mooter, Vauterin, Hoste, Gillis, Mew, and
Kersters 1990
Soybean bacterial blight: Pseudomonas savastanoi pv. glycinea (Coerper
1919) Gardan, Bollet, Abu Ghorrah, Grimont, and Grimont 1992
(Pseudomonas syringae pv. glycinea [Coerper 1919] Young, Dye, and
Wilkie 1978)*
Tomato bacterial canker: Clavibacter michiganensis ssp. michiganensis
(Smith 1910) Davis, Gillaspie, Vidaver, and Harris 1984
Tomato bacterial speck: Pseudomonas syringae pv. tomato (Okabe 1933)
Young, Dye, and Wilkie 1978
Tomato bacterial spot: Xanthomonas vesicatoria (ex Doidge 1920)
Vauterin, Hoste, Kersters, and Swings 1995 (X. campestris pv.
vesicatoria [Doidge 1920] Dye 1978)*
Tomato bacterial stem rot and fruit rot: Erwinia carotovora ssp.
carotovora (Jones 1902) Bergey, Harrison, Breed, Hammer, and
Huntoon 1923
Tomato bacterial wilt: Ralstonia solanacearum (Smith 1896) Yabauuchi,
Kosako, Yano, Hotta, and Nishiuchi 1995
CLASSIFICATION OF PLANT BACTERIAL PATHOGENS
Bacteria have been classified into various genera, families, suborders, orders, subclasses, classes, divisions, and domains. These classifications are
based on numerical analyses, serology, membrane protein profiles, and
DNA analyses. In contrast to bacterial nomenclature, no official classification of bacteria exists. Taxonomy remains a matter of scientific judgment
and general agreement. The classification given here is the one widely accepted by microbiologists (Euzeby, 2001). Bacteria are classified into domain or empire (suffix not covered by rules), division (suffix not covered by
the rules), class (suffix of the names of class is ia), subclass (suffix
is idae), order (suffix is ales), suborder (-ineae), family (-aceae), and tribe
(-eae). The taxonomic categories of domain and division are not covered by
the rules of bacteriological code. The following 17 families include 28 genera of plant-pathogenic bacteria:
Acetobacteraceae: Acetobacter, Gluconobacter
Bacillaceae: Bacillus
Burkholderiaceae: Burkholderia
Clostridiaceae: Clostridium
Comamonadaceae: Acidovorax
Corynebacteriaceae: Corynebacterium
Enterobacteriaceae: Erwinia, Pantoea, Pectobacterium,
Enterobacter, Serratia
Microbacteriaceae: Curtobacterium, Clavibacter, Rathayibacter
Micrococcaceae: Arthrobacter
Nocardiaceae: Nocardia, Rhodococcus
Pseudomonadaceae: Pseudomonas, Xylophilus, Rhizobacter
Ralstoniaceae: Ralstonia
Rhizobiaceae: Agrobacterium
Sphingomonadaceae: Rhizomonas
Spiroplasmataceae: Spiroplasma
Streptomycetaceae: Streptomyces
Xanthomonadaceae: Xanthomonas, Xylella
The following 12 orders contain 17 families consisting of plant-pathogenic bacteria:

Actinomycetales: Corynebacteriaceae, Microbacteriaceae,
Nocardiaceae, Streptomycetaceae
Bacillales: Bacillaceae
Burkholderiales: Burkholderiaceae, Ralstoniaceae,
Comamonadaceae
Clostridiales: Clostridiaceae
Enterobacteriales: Enterobacteriaceae
Entomoplasmatales: Spiroplasmataceae
Micrococcales: Micrococcaceae
Pseudomonadales: Pseudomonadaceae
Rhizobiales: Rhizobiaceae
Rhodospirillales: Acetobacteraceae
Sphingomonadales: Sphingomonadaceae
Xanthomonadales: Xanthomonadaceae
The following six classes contain 12 orders consisting of plant bacterial
pathogens:
Amoxyphotobacteria: Rhodospirillales
Bacilli: Bacillales
Clostridia: Clostridiales
Mollicutes: Entomoplasmatales
Proteobacteria:
Alpha subdivision: Sphingomonadales, Rhizobiales
Beta subdivision: Burkholderiales
Gamma subdivision: Enterobacteriales, Xanthomonadales,
Pseudomonadales
Schizomycetes: Actinomycetales, Micrococcales
Two divisions have been recognized to include some classes consisting
of bacterial pathogens:
Firmicutesclass Mollicutes (order Entomoplasmatales, family
Spiroplasmataceae, genus Spiroplasma).
Gracilicutesclass Anoxyphotobacteria (order Rhodospirillales,
family Acetobacteraceae, genus Gluconobacter)
Some bacteria have not been described in sufficient detail to warrant establishment of a novel taxon. These bacteria have been included in a new
category of indefinite rank called Candidatus (Murray and Schleifer, 1994).
Candidatus is used to categorize prokaryotic entities for which more than

one sequence is available, but for which characteristics required for description according to the International Code of Nomenclature of Bacteria
(ICSB, 1992) are lacking. Candidatus Liberobacter africanum Jagoueix
et al., 1994, and C. L. asiaticum are the phloem-limited bacteria causing
greening disease of Citrus. They are members of the Alpha subdivision of
the Proteobacteria.
SYSTEMATIC POSITION OF CROP BACTERIAL PATHOGENS
The following is a list of bacterial genera that cause diseases in crops.
Systematic positions and names of important species in each genus are
given. The names of many bacterial species were changed recently (Young
et al., 1996; Euzeby, 2001). The most important plant pathogenic genus,
Pseudomonas, was reclassified into five different genera, including Pseudomonas, Burkholderia, Ralstonia, Acidovorax, and Acetobacter. The genus
Xanthomonas was reclassified into two genera, Xanthomonas and Xylophilus. The genus Erwinia was reassigned into four different genera: Erwinia, Pantoea, Enterobacter, and Serratia. The genus Corynebacterium
was not recognized, but remains a valid synonym of the genera Curtobacterium, Clavibacter, Erwinia, Rhodococcus, Arthrobacter, and Rathayibacter. The names of various bacterial species as recommended by Young
et al. (1996) are provided. Suggested modifications in the names of bacterial
species (Schaad et al., 2000) are marked with an asterisk (*). Valid synonyms of some bacterial species are given as = (without asterisk).
AcetobacterTribe: Acetobactereae; Family: Acetobacteraceae; Order:
Rhodospirillales; Class: Anoxyphotobacteria; Division: Gracilicutes;
Domain: Bacteria
Acetobacter pasteurianus (Hansen 1879) Beijerinck and Folpmers
1916 = Pseudomonas pomi Cole 1959
AcidovoraxFamily: Comamonadaceae; Order: Bukholderiales; Class:
Proteobacteria Beta Subdivision; Domain: Bacteria
Acidovorax avenae ssp. avenae (Manns 1909) Willems et al. 1992 =
Pseudomonas avenae ssp. avenae 1909
A. avenae ssp. citrulli (Schaad et al. 1978) Willems et al. 1992 = P.
avenae ssp. citrulli (Schaad et al. 1978) Hu et al. 1991
AgrobacteriumFamily: Rhizobiaceae; Order: Rhizobiales; Class:
Proteobacteria Alpha subdivision; Domain: Bacteria
Agrobacterium rhizogenes (Riker et al. 1930) Conn 1942
A. rubi (Hildebrand 1940) Starr and Weiss 1943
A. tumefaciens (Smith and Townsend 1907) Conn 1942

= A. radiobacter (Beijerinck and van Delden 1902) Conn 1942
A. vitis Ophel and Kerr 1990
ArthrobacterFamily: Micrococcaceae; Order: Micrococcales; Class:
Schizomycetes; Domain: Bacteria
Arthrobacter ilicis (Mandel et al. 1961) Collins et al. 1982 =
Corynebacterium ilicis Mandel et al. 1961
BacillusFamily: Bacillaceae; Order: Bacillales; Class: Bacilli; Division:
Firmicutes; Domain: Bacteria
Bacillus megaterium pv. cerealis Hosford 1982
BurkholderiaFamily: Burkholderiaceae; Order: Burkholderiales; Class:
Proteobacteria Beta subdivision; Domain: Bacteria
Burkholderia caryophylli (Burkholder 1942) Yabuuchi et al. 1993 =
Pseudomonas caryophylli (Burkholder 1942) Starr and
Burkholder 1942
B. cepacia (ex Burkholder 1950) = P. cepacia (ex Burkholder 1950)
Palleroni and Holmes 1981
B. gladioli pv. gladioli (Severini 1913) Yabuuchi et al. 1992 = P.
gladioli pv. gladioli (Severini 1913)
CandidatusClass: Proteobacteria Alpha subdivision; Domain: Bacteria
(Phloem-limited plant bacteria)
Candidatus Liberobacter africanum Jagoueix et al., 1994
Candidatus Liberobacter asiaticum Jagoueix et al., 1994
ClavibacterFamily: Microbacteriaceae; Suborder: Micrococcineae; Order: Actinomycetales; Subclass: Actinobacteridae; Class:
Clavibacter michiganensis ssp. insidiosus (McCulloch 1925) Davis
et al. 1984 = Corynebacterium michiganense ssp. insidiosum
(McCulloch 1925) Carlson and Vidaver 1982
Clavibacter michiganensis ssp. sepedonicus (Spieckermann and
Kotthoff 1914) Davis et al. 1984 = Corynebacterium
michiganense ssp. sepedonicum (Spieckermann and Kotthoff
1914) Carlson and Vidaver 1982
Clavibacter xyli Davis et al. 1984
ClostridiumFamily: Clostridiaceae; Order: Clostridiales; Class:
Clostridia; Domain: Bacteria
Clostridium puniceum Lund et al. 1981
CorynebacteriumFamily: Corynebacteriaceae; Suborder:
Corynebacterineae; Order: Actinomycetales; Subclass:
Actinobacteridae; Class: Schizomycetes; Domain: Bacteria. In the revised nomenclature, the bacteria belonging to the genus
Corynebacterium were assigned to the genera Curtobacterium,

Erwinia, Rhodococcus, Arthrobacter, Clavibacter, and Rathayibacter.
CurtobacteriumFamily: Microbacteriaceae; Suborder: Micrococcineae;
Order: Actinomycetales; Subclass: Actinobacteridae; Class:
Curtobacterium flaccumfaciens pv. betae (Keyworth et al. 1956)
Collins and Jones 1983 = Corynebacterium flaccumfaciens pv.
betae (Keyworth et al. 1956) Dye and Kemp 1977
Curtobacterium flaccumfaciens pv. flaccumfaciens (Hedges 1922)
Collins and Jones 1983 = Corynebacterium flaccumfaciens pv.
flaccumfaciens (Hedges 1922) Dowson 1942
Curtobacterium flaccumfaciens pv. poinsettiae (Starr and Pirone
1942) Collins and Jones 1983 = Corynebacterium flaccumfaciens
pv. poinsettiae (Starr and Pirone 1942) Dye and Kemp 1977
EnterobacterFamily: Enterobacteriaceae; Order: Enterobacteriales;
Class: Proteobacteria Gamma subdivision; Domain: Bacteria
Enterobacter dissolvens (Rosen 1922) Brenner et al. 1988 = Erwinia
dissolvens (Rosen 1922) Bukholder 1948
ErwiniaFamily: Enterobacteriaceae; Suborder: Erwinieae; Order:
Enterobacteriales; Class: Proteobacteria Gamma subdivision; Domain:
Bacteria
Erwinia amylovora (Burrill 1882) Winslow et al. 1920
E. carotovora ssp. atroseptica (van Hall 1902) Dye 1969
E. carotovora ssp. betavasculorum Thomson et al. 1923
E. carotovora ssp. carotovora (Jones 1901) Bergey et al. 1923
E. chrysanthemi pv. chrysanthemi Burkholder et al. 1953 =
Pectobacterium chrysanthemi (Burkholder et al. 1953) Brenner
et al. 1973
E. chrysanthemi pv. dianthicola (Hellmers 1958) Dickey 1979
E. chrysanthemi pv. dieffenbachiae (McFadden 1961) Dye 1978
E. chrysanthemi pv. zeae (Sabet 1954) Victoria et al. 1975
E. herbicola pv. gypsophilae (Brown 1934) Miller et al. 1981
E. psidii Neto et al. 1988
E. quercina Hildebrand and Schroth 1967
E. rhapontici (Millard 1924) Burkholder 1948
E. tracheiphila (Smith 1895) Bergey et al. 1923
GluconobacterFamily: Acetobacteraceae; Order: Rhodospirillales;
Class: Anoxyphotobacteria; Division: Gracilicutes; Domain: Bacteria
Gluconobacter oxydans (Henneberg 1897) De Ley 1961
NocardiaFamily: Nocardiaceae; Suborder: Corynebacterineae; Order:
Actinomycetales; Subclass: Actinobacteridae; Class: Schizomycetes;
Domain: Bacteria
Nocardia vaccini Demaree and Smith 1952
PantoeaFamily: Enterobacteriaceae; Order: Enterobacteriales; Class:

Proteobacteria Gamma subdivision; Domain: Bacteria
Pantoea agglomerans (Beijerinck 1888) Gavini et al. 1989 =
Erwinia herbicola (Lohnis 1911) Dye 1964
P. ananas pv. ananas (Serrano 1928) Mergaert et al. 1993 = E.
ananas pv. ananas Serrano 1928
P. stewartii ssp. stewartii (Smith 1898) Mergaert et al. 1993 = E.
stewartii (Smith 1898) Dye 1963
PectobacteriumFamily: Enterobacteriaceae; Order: Enterobacteriales;
Pectobacterium spp. are now synonyms of various Erwinia spp.
PseudomonasTribe: Pseudomonadeae; Family: Pseudomonadaceae;
Suborder: Pseudomonadineae; Order: Pseudomonadales; Class:
Pseudomonas amygdali Psallidas and Panagopoulos 1975
P. caricapapayae Robbs 1956
P. cichorii (Swingle 1925) Stapp 1928
P. corrugata (ex Scarlett et al. 1978) Roberts and Scarlett 1981
P. fuscovaginae (ex Tanii et al. 1976) Miyajima et al. 1983
P. marginalis pv. alfalfae (Shinde and Lukezic 1974) Young et al.
1978
P. marginalis pv. marginalis (Brown 1918) Stevens 1925
P. meliae Ogimi 1981
P. savastanoi pv. glycinea (Coerper 1919) Gardan et al. 1992 = P.
syringae pv. glycinea (Coerper 1919) Young et al. 1978*
P. savastanoi pv. phaseolicola (Burkholder 1926) Gardan et al. 1992
= P. syringae pv. phaseolicola (Burkholder 1926) Young et al.
1978*
P. savastanoi pv. savastanoi (ex Smith 1908) Gardan et al. 1992 = P.
savastanoi*
P. syringae pv. antirrrhini (Takimoto 1920) Young, Dye, and Wilkie
1978
P. syringae pv. apii (Jagger 1921) Young, Dye, and Wilkie 1978
P. syringae pv. aptata (Brown and Jamieson 1913) Young, Dye, and
Wilkie 1978
P. syringae pv. atrofaciens (McCulloch 1920) Young, Dye, and
Wilkie 1978
P. syringae pv. atropurpurea (Reddy and Godkin 1923) Young, Dye,
and Wilkie 1978
P. syringae pv. cannabina (Sutic and Dowson 1959) Young, Dye,
and Wilkie 1978
P. syringae pv. castaneae Takanashi and Shimizu 1989
P. syringae pv. coronafaciens (Elliott 1920) Young, Dye, and Wilkie
1978
P. syringae pv. delphinii (Smith 1904) Young, Dye, and Wilkie 1978
P. syringae pv. helianthi (Kawamura 1934) Young, Dye, and Wilkie
1978
P. syringae pv. lachrymans (Smith and Bryan 1915) Young, Dye,
and Wilkie 1978
P. syringae pv. maculicola (McCulloch 1911) Young, Dye, and
Wilkie 1978
P. syringae pv. mellea (Johnson 1923) Young, Dye, and Wilkie 1978
P. syringae pv. mori (Boyer and Lambert 1893) Young, Dye, and
Wilkie 1978
P. syringae pv. morsprunorum (Wormald 1931) Young, Dye, and
Wilkie 1978
P. syringae pv. oryzae (Kuwata 1985) Young et al. 1991
P. syringae pv. populans (Rose 1917) Dhanvantari 1977
P. syringae pv. passiflorae (Reid 1938) Young, Dye, and Wilkie
1978
P. syringae pv. persicae (Prunier et al. 1970) Young, Dye, and
Wilkie 1978
P. syringae pv. pisi (Sackett 1916) Young, Dye, and Wilkie 1978
P. syringae pv. sesami (Malkoff 1906) Young, Dye, and Wilkie 1978
P. syringae pv. striafaciens (Elliott 1927) Young, Dye, and Wilkie
1978
P. syringae pv. syringae van Hall 1902
P. syringae pv. tabaci (Wolf and Foster 1917) Young, Dye, and
Wilkie 1978
P. syringae pv. tagetis (Hellmers 1955) Young, Dye, and Wilkie
1978
P. syringae pv. theae (Hori 1915) Young, Dye, and Wilkie 1978
P. syringae pv. tomato (Okabe 1933) Young, Dye, and Wilkie 1978
P. syringae pv. ulmi (Sutic and Tesic 1958) Young, Dye, and Wilkie
1978
P. syringae pv. viburni (Thornberry and Anderson 1931) Young,
Dye, and Wilkie 1978
P. syringae pv. zizaniae (ex Bowden and Pereich 1983) Young et al.
1991
P. viridiflava (Burkholder 1930) Dowson 1939
RalstoniaFamily: Ralstoniaceae; Order: Burkholderiales; Class:
Proteobacteria Beta subdivision; Domain: Bacteria
Ralstonia solanacearum (Smith 1896) Yabauuchi et al. 1995 =
Pseudomonas solanacearum (Smith 1896) Smith 1914 =
Burkholderia solanacearum (Smith 1896) Yabauuchi et al. 1993
RathayibacterFamily: Microbacteriaceae; Suborder: Micrococcineae;

Order: Actinomycetales; Subclass: Actinobacteridae; Class:
Rathayibacter rathayi (Smith 1913) Zgurskaya et al. 1993 =
Clavibacter rathayi (Smith 1913) Davis et al. 1984 =
Corynebacterium rathayi (Smith 1913) Dowson 1942
R. tritici (ex Hutchinson 1917) Zgurskaya et al. 1993
RhizobacterFamily: Pseudomonadaceae; Suborder: Pseudomonadineae;
Order: Pseudomonadales; Class: Proteobacteria Gamma subdivision;
Domain: Bacteria
Rhizobacter dauci Goto and Kuwata 1988
RhizomonasFamily: Sphingomonadaceae; Order: Sphingomonadales;
Class: Proteobacteria Alpha subdivision; Domain: Bacteria
Rhizomonas suberifaciens van Bruggen, Jochimsen and Brown 1990
RhodococcusFamily: Nocardiaceae; Suborder: Corynebacterineae; Order: Actinomycetales; Subclass: Actinobacteridae; Class:
Rhodococcus fascians (Tilford 1936) Goodfellow 1984 =
Corynebacterium fascians (Tilford 1936) Dowson 1942
SerratiaTribe: Serratiaceae; Family: Enterobacteriaceae; Order:
Enterobacteriales; Class: Proteobacteria Gamma subdivision; Domain:
Bacteria
Serratia proteamaculans (Paine and Stansfield 1919) Grimont,
Grimont, and Starr 1978 = Erwinia proteamaculans (Paine and
Stansfield 1919) Dye 1966
SpiroplasmaFamily: Spiroplasmataceae; Order: Entomoplastales;
Class: Mollicutes; Division: Firmicutes; Domain: Bacteria
Spiroplasma citri Saglio, Lhospital, Lafleche, Dupont, Bove, Tully,
and Freundt 1973
S. kunkelii Whitcomb et al. 1986
S. phoeniceum Saillard et al. 1987
StreptomycesFamily: Streptomycetaceae; Order: Actinomycetales;
Actinobacteridae; Class: Schizomycetes; Domain: Bacteria
Streptomyces scabies (ex Thaxter 1892) Lambert and Loria 1989
S. ipomoeae (Person and Martin 1940) Waksman and Henrici 1948
XanthomonasFamily: Xanthomonadaceae; Order: Xanthomonadales;
Xanthomonas albilineans (Ashby 1929) Dowson 1943
X. arboricola pv. juglandis (Pierce 1901) Vauterin et al. 1995 = X.
juglandis pv. juglandis*
X. arboricola pv. populi (ex de Kam 1984) Vauterin et al. 1995 = X.
campestris pv. populi (ex de Kam 1984) Young et al. 1991
X. arboricola pv. pruni (Smith 1903) Vauterin et al. 1995 = X.

juglandis pv. pruni*
X. arboricola pv. corylina (Miller et al. 1940) Vauterin et al. 1995 =
X. juglandis pv. corylina*
X. axonopodis pv. alfalfae (Riker et al. 1935) Vauterin et al. 1995 =
X. campestris pv. alfalfae ((Riker et al. 1935) Dye 1978*
X. axonopodis pv. axonopodis Starr and Garces 1950
X. axonopodis pv. bauhiniae (Padhya et al. 1965) Vauterin et al.
1995 = X. campestris pv. bauhiniae (Padhya et al. 1965) Dye
1978*
X. axonopodis pv. begoniae (Takimoto 1934) Vauterin et al. 1995 =
X. campestris pv. begoniae (Takimoto 1934) Dye 1978*
X. axonopodis pv. cajani (Kulkarni et al. 1950) Vauterin et al. 1995
= X. campestris pv. cajani (Kulkarni et al. 1950) Dye 1978*
X. axonopodis pv. cassiae (Kulkarni et al. 1951) Vauterin et al. 1995
= X. campestris pv. cassiae (Kulkarni et al. 1951) Dye 1978*
X. axonopodis pv. citri (Hasse 1915) Vauterin et al. 1995 = X.
campestris pv. citri (Hasse 1915) Dye 1978*
X. axonopodis pv. clitoriae (Pandit and Kulkarni 1979) Vauterin
et al. 1995 = X. campestris pv. clitoriae (Pandit and Kulkarni
1979) Dye et al. 1980*
X. axonopodis pv. coracanae (Desai et al. 1965) Vauterin et al. 1995
= X. campestris pv. coracanae (Desai et al. 1965) Dye 1978*
X. axonopodis pv. cyamopsidis (Patel et al. 1953) Vauterin et al.
1995 = X. campestris pv. cyamopsidis (Patel et al. 1953) Dye
1978*
X. axonopodis pv. desmodii (Patel 1949) Vauterin et al. 1995 = X.
campestris pv. desmodii (Patel 1949) Dye 1978*
X. axonopodis pv. desmodiilaxiflori (Pant and Kulkarni 1976)
Vauterin et al. 1995 = X. campestris pv. desmodiilaxiflori Pant
and Kulkarni 1976*
X. axonopodis pv. desmodiirotundifolii (Deasi and Shah 1960)
Vauterin et al. 1995 = X. campestris pv. desmodiirotundifolii
(Deasi and Shah 1960) Dye 1978*
X. axonopodis pv. dieffenbachiae (McCulloch and Pirone 1939)
Vauterin et al. 1995 = X. campestris pv. dieffenbachiae
(McCulloch and Pirone 1939) Dye 1978*
X. axonopodis pv. erythrinae (Patel et al. 1952) Vauterin et al. 1995
= X. campestris pv. erythrinae (Patel et al. 1952) Dye 1978*
X. axonopodis pv. glycines (Nakano 1919) Vauterin et al. 1995 = X.
campestris pv. glycines (Nakano 1919) Dye 1978*
X. axonopodis pv. lespedezae (Ayres et al. 1939) Vauterin et al. 1995

= X. campestris pv. lespedezae (Ayres et al. 1939) Dye 1978*
X. axonopodis pv. malvacearum (Smith 1901) Vauterin et al. 1995 =
X. campestris pv. malvacearum (Smith 1901) Dye 1978*
X. axonopodis pv. manihotis (Bondar 1915) Vauterin et al. 1995 = X.
campestris pv. manihotis (Bondar 1915) Dye 1978*
X. axonopodis pv. patelii (Desai and Shah 1959) Vauterin et al. 1995
= X. campestris pv. patelii (Desai and Shah 1959) Dye 1978*
X. axonopodis pv. phaseoli (Smith 1897) Vauterin et al. 1995 = X.
campestris pv. phaseoli (Smith 1897) Dye 1978*
X. axonopodis pv. phyllanthi (Sabet et al. 1969) Vauterin et al. 1995
= X. campestris pv. phyllanthi (Sabet et al. 1969) Dye 1978*
X. axonopodis pv. poinsettiicola (Patel et al. 1951) Vauterin et al.
1995 = X. campestris pv. poinsettiicola (Patel et al. 1951) Dye
1978*
X. axonopodis pv. rhynchosiae (Sabet et al. 1969) Vauterin et al.
1995 = X. campestris pv. rhynchosiae (Sabet et al. 1969) Dye
1978*
X. axonopodis pv. ricini (Yoshii and Takimoto 1928) Vauterin et al.
1995 = X. campestris pv. ricini (Yoshii and Takimoto 1928) Dye
1978*
X. axonopodis pv. sesbaniae (Patel et al. 1952) Vauterin et al. 1995
= X. campestris pv. sesbaniae (Patel et al. 1952) Dye 1978*
X. axonopodis pv. tamarindi (Patel et al. 1951) Vauterin et al. 1995
= X. campestris pv. tamarindi (Patel et al. 1951) Dye 1978*
X. axonopodis pv. vasculorum (Cobb 1894) Vauterin et al. 1995 = X.
campestris pv. vasculorum (Cobb 1894) Dye 1978*
X. axonopodis pv. vignaeradiatae (Sabet et al. 1969) Vauterin et al.
1995 = X. campestris pv. vignaeradiatae (Sabet et al. 1969) Dye
1978*
X. axonopodis pv. vignicola (Burkholder 1944) Vauterin et al. 1995
= X. campestris pv. vignicola (Burkholder 1944) Dye 1978*
X. axonopodis pv. vitians (Brown 1918) Vauterin et al. 1995 = X.
campestris pv. vitians (Brown 1918) Dye 1978*
X. bromi Vauterin et al. 1995 = X. campestris pv. bromi*
X. campestris pv. aberrans (Knosel 1961) Dye 1978
X. campestris pv. aracearum (Berniac 1974) Dye 1978
X. campestris pv. arecae (Rao and Mohan 1970) Dye 1978
X. campestris pv. argemones (Srinivasan et al. 1961) Dye 1978
X. campestris pv. armoraciae (McCulloch 1929) Dye 1978
X. campestris pv. arracaciae (Pereira et al. 1971) Dye 1978
X. campestris pv. asclepiadis Flynn and Vidaver 1990
X. campestris pv. azadirachtae (Desai et al. 1966) Dye 1978

X. campestris pv. betae Robbs et al. 1981
X. campestris pv. campestris (Pammel 1895) Dowson 1939
X. campestris pv. carissae (Moniz et al. 1964) Dye 1978
X. campestris pv. eucalypti (Truman 1974) Dye 1978
X. campestris pv. euphorbiae (Sabet et al. 1969) Dye 1978
X. campestris pv. fici (Cavara 1905) Dye 1978
X. campestris pv. guizotiae (Yirgou 1964) Dye 1978
X. campestris pv. incanae (Kendrick and Baker) Dye 1978
X. campestris pv. leersiae (ex Fang et al. 1957) Young et al. 1991
X. campestris pv. mangiferaeindicae (Patel et al. 1948) Robbs et al.
1974
X. campestris pv. musacearum (Yirgou and Bradbury 1968) Dye
1978
X. campestris pv. nigromaculans (Takimoto 1927) Dye 1978
X. campestris pv. olitorii (Sabet 1957) Dye 1978
X. campestris pv. papavericola (Bryan and McWhorter 1930) Dye
1978
X. campestris pv. passiflorae (Pereira 1969) Dye 1978
X. campestris pv. paulliniae Robbs et al. 1982
X. campestris pv. phormiicola (Takimoto 1933) Dye 1978
X. campestris pv. plantaginis (Thornberry and Anderson 1937) Dye
1978
X. campestris pv. raphani (White 1930) Dye 1978
X. campestris pv. sesami (Sabet and Dowson 1960) Dye 1978
X. campestris pv. syngonii Dickey and Zumoff 1987
X. campestris pv. viticola (Nayudu 1972) Dye 1978
X. campestris pv. vitiscarnosae (Moniz and Patel 1958) Dye 1978
X. campestris pv. vitistrifoliae (Padhya et al. 1965) Dye 1978
X. campestris pv. vitiswoodrowii (Patel and Kulkarni 1951) Dye
1978
X. campestris pv. zingibericola (Ren and Fang 1981) Bradbury 1986
X. campestris pv. zinniae (Hopkins and Dowson 1949) Dye 1978
X. cassavae (ex Wiehe and Dowson 1953) Vauterin et al. 1995
X. cucurbitae (ex Bryon 1926) Vauterin et al. 1995
X. exitiosa (Gardner and Kendrick 1921) Schaad et al. 2000
X. fragariae Kennedy and King 1962
X. hederae (Arnaud 1920) Schaad et al. 2000
X. hortorum pv. hederae (Arnaud 1920) Vauterin et al. 1995 = X.
hederae pv. hederae Schaad et al. 2000*
X. hortorum pv. hortorum Vauterin et al. 1995
X. hortorum pv. pelargonii (Brown 1923) Vauterin et al. 1995 = X.

hederae pv. pelargonii Schaad et al. 2000*
X. hyacinthi (Wakker 1883) Vauterin et al. 1995
X. melonis (Neto et al. 1984) Vauterin et al. 1995 = X. campestris
pv. melonis (Neto et al. 1984)*
X. oryzae pv. oryzae (Ishiyama 1922) Swings et al. 1990
X. oryzae pv. oryzicola (Fang et al. 1957) Swings et al. 1990
X. sacchari Vauterin et al. 1995 = X. albilineans Schaad et al. 2000*
X. theicola (Uehara et al. 1980) Vauterin et al. 1995 = X. campestris
pv. theicola (Uehara et al., 1980)*
X. translucens pv. arrhenatheri (Egli and Schmidt 1982) Vauterin
et al. 1995 = X. campestris pv. arrhenatheri Egli and Schmidt
1982*
X. translucens pv. graminis (Egli et al. 1975) Vauterin et al. 1995 =
X. campestris pv. graminis (Egli et al. 1975) Dye 1978*
X. translucens pv. phlei (Egli and Schmidt 1982) Vauterin et al.
1995 = X. campestris pv. phlei Egli and Schmidt 1982*
X. translucens pv. phleipratensis (Wallin and Reddy 1945) Vauterin
et al. 1995 = X. campestris pv. phleipratensis (Wallin and Reddy
1945) Dye 1978*
X. translucens pv. poae (Egli and Schmidt 1982) Vauterin et al.
1995 = X. campestris pv. poae Egli and Schmidt 1982
X. vasicola pv. holcicola (Elliott 1930) Vauterin et al. 1995 = X.
campestris pv. holcicola Schaad et al. 2000*
X. vasicola pv. vasculorum Vauterin et al. 1995 = X. campestris pv.
vasculorum (Cobb 1894) Dye 1978
X. vesicatoria (ex Doidge 1920) Vauterin et al. 1995 = X. campestris
pv. vesicatoria (ex Doidge 1920) Dye 1978*
XylellaFamily: Xanthomonadaceae; Order: Xanthomonadales; Class:
Xylella fastidiosa Wells, Raju, Hung, Weisburg, MandelcoPaul,
and Brenner 1987 (Xylem-limited fastidious plant bacteria)
XylophilusFamily: Pseudomonadaceae; Suborder: Pseudomonadineae;
Order: Pseudomonadales; Class: Proteobacteria Gamma subdivision;
Domain: Bacteria
Xylophilus ampelinus (Panagopoulos 1969) Willems et al. 1987 =
Xanthomonas ampelina Panagopoulos 1969
REFERENCES
Euzeby, J. P. (2001). Taxonomic Scheme. Taxa included in the categories above the
rank of genus. Web site: <http://www.bacterio.cict.fr/classification.html>.
International Committee on Systematic Bacteriology (ICSB) (1992). International

code of nomenclature of bacteria: Bacteriological code (1990 revision). Washington, DC: American Society for Microbiology.
Murray, R. G. E. and Schleifer, K. H. (1994). Taxonomic notes: A proposal for recording the properties of putative taxa of prokaryotes. Int J Syst Bacteriol,
44:174-176.
Schaad, N. W., Vidaver, A. K., Lacy, G. H., Rudolph, K., and Jones, J. B. (2000).
Evaluation of proposed amended names of several pseudomonads and xanthomonads and recommendations. Phytopathology, 90:208-213.
Young, J. M., Saddler, G. S., Takikawa, Y., De Boer, S. H., Vauterin, L., Gardan,
L., Gvozdyak, R. I., and Stead, D. E. (1996). Names of plant pathogenic bacteria
1864-1995. Rev Plant Pathol, 75:721-761.
Fungi,Fungi,
Including
IncludingChromista
Chromista andand
Protozoa
Protozoa
Fungi are the largest group of crop pathogens. Fungi belong to three
kingdoms: Fungi, Chromista, and Protozoa. The Kingdom Chromista includes the phylum Oomycota, which contains many plant-pathogenic fungi.
The Kingdom Protozoa consists of one phylum, Plasmodiophoromycota,
which contains crop pathogens. The Kingdom Fungi consists of four phyla:
Ascomycota, Basidiomycota, Zygomycota, and Chytridiomycota. Another
group, Mitosporic fungi, includes fungi that have not been correlated with
any meiotic states. This section describes the structure and reproduction of
all these fungal phyla. It also provides a complete list of crop fungal pathogens and their systematic positions.
THREE KINGDOMS CONTAINING FUNGI:
FUNGI, CHROMISTA, AND PROTOZOA
Fungi are the most important group of plant pathogens. Out of about
56,360 species of fungi, more than 8,000 species are known to cause diseases in plants. Originally all fungi were classified as belonging to the Kingdom Fungi (= Eukaryota). Recently some fungi have been considered not to
belong to this kingdom and have been placed under the Kingdoms Chromista and Protozoa. With advances in ultrastructural, biochemical, and especially molecular biology, the treatment of fungi as a single kingdom has
become untenable. The organisms so far called fungi are now established as
polyphyletic (i.e., with different phylogenies) and have to be referred to
three different kingdoms (Hawksworth et al., 1995): Fungi, Chromista, and
Protozoa. The Kingdom Fungi contains true fungi while the Kingdoms
Chromista and Protozoa contain pseudofungi. The Kingdom Fungi consists exclusively of fungi; the Chromista and Protozoa mainly comprise
nonfungal phyla. Chromista consists of three fungal phyla and Protozoa
consists of only four fungal phyla.
Fungi are organisms that are eukaryotic and heterotrophic. They develop
branching filaments (or more rarely are single-celled), reproduce by spores,
and their cell walls contain chitin and -glucans. They are mostly nonflag-
ellate. When present, the flagella always lack mastigonemes (i.e., surfaces
of flagella are not covered by hairlike processes). Chromista are organisms
that bear flagella with mastigonemes. Their cell walls contain cellulose and
glucan rather than chitin. Mycolaminarin is the energy storage molecule
found in Chromista. Protozoa are organisms that are predominantly unicellular, plasmodial, or colonial. They are phagotrophic, i.e., they feed by ingestion, engulfing food. They are wall-less in the trophic state. They have
ciliary hairs that are never rigid or tubular.
OOMYCOTA
Classification of Oomycota
Three fungal (pseudofungal) phyla that belong to Chromista are Hypochytriomycota, Labyrinthulomycota, and Oomycota. Among them, only
Oomycota consists of plant pathogens. There are more than 500 species in
Oomycota. These include so-called water molds and downy mildews. Oomycota means egg fungi and refers to the large round oogonia (the structures containing the female gametes). Oomycota are oogamous, producing
large nonmotile gametes called eggs, and smaller gametes called sperm.
Oomycota were classified previously as fungi because of their filamentous
growth. However, Oomycota cell walls are not composed of chitin, as in the
fungi, but are made up of a mix of cellulosic compounds and glycan. The
nuclei within the filaments are diploid, with two sets of genetic information,
not haploid as in the fungi. The ultrastructure, biochemistry, and molecular
sequences of Oomycota suggest that they belong with Chromista. The freeswimming spores that are produced bear two dissimilar flagella, one with
mastigonemes. This feature is common in chromists. Presence of the chemical
mycolaminarin in Oomycota is similar to that found in kelps and diatoms.
Hence, Oomycota have been placed in the Kingdom Chromista.
The phylum (division) Oomycota consists of nine orders: Olpidiopsidales, Peronosporales, Pythiales, Sclerosporales, Saprolegniales, Leptomidales, Myzocytiopsidales, Rhipidiales, and Salilagenidales. Only the orders Peronosporales, Pythiales, Sclerosporales, and Saprolegniales contain
crop pathogens. Oomycota contains many important pathogens that have affected the economies of many countries. In the order Pythiales, the family
Pythiaceae contains various Phytophthora and Pythium species that cause
several diseases in various crops. Phytophthora infestans causes late blight
in potato and tomato. Phytophthora citrophthora is the causal organism of
gummosis and foot rot diseases in Citrus. Phytophthora capsici causes
blight in pepper, P. cactorum incites crown and root rot in apple and peach,
P. parasitica pv. nicotianae causes black shank in tobacco, and P. sojae

causes root and stem rot of soybean. Pythium debaryanum, P. ultimum, and
P. aphanidermatum cause damping-off of vegetables and fruit trees. Pythium arrhenomanes, P. graminicola, and P. tardicrescens cause root rot in cereals.
In the order Peronosporales, the family Albuginaceae contains Albugo
candida (white rust of crucifers) and Albugo ipomoeae-panduranae (white
rust of sweet potato). In the order Peronosporales, the family Peronosporaceae contains Plasmopara viticola (downy mildew of grapevine),
Peronospora parasitica (downy mildew of crucifers), Pseudoperonospora
cubensis (downy mildew of cucurbits), and Bremia lactucae (downy mildew of lettuce).
In the order Sclerosporales, the family Sclerosporaceae contains Peronosclerospora sorghi (downy mildew of corn and sorghum), Sclerospora
graminicola (green ear and downy mildew of corn), P. philippinensis (Philippine downy mildew of corn), and P. sacchari (sugarcane downy mildew).
In the order Sclerosporales, the family Verrucalvaceae contains Sclerophthora macrospora (crazy-top downy mildew of corn) and S. rayssiae var.
zeae (brown-stripe downy mildew of corn). In the order Saprolegniales, the
family Saprolegniaceae contains the crucifer black-root pathogen Aphanomyces raphani and the pea root-rot pathogen A. euteiches.
According to FungalWeb (n.d.) <http://www.fungalweb.com>, Oomycetes is recognized as a class by an international group of mycologists who met
in Copenhagen in October 1999. It consists of the orders Leptomitales, Peronosporales, Pythiales, Rhipidales, Saprolegniales, and Sclerosporales. The orders Leptomitales and Rhipidales do not have any plant pathogens. The order
Peronosporales consists of the families Peronosporaceae (Peronospora, Plasmopara, Bremia) and Albuginaceae (Albugo). The order Pythiales consists
of the family Pythiaceae (Phytophthora, Pythium), the order Saprolegniales
consists of the family Saprolegniaceae (Aphanomyces), and the order Sclerosporales consists of the families Sclerosporaceae (Peronosclerospora, Sclerospora) and Verrucalvaceae (Sclerophthora).
Structure of Oomycota
The vegetative body of a fungus is called the mycelium. The mycelium is
the mass of hyphae that constitutes the thallus. Hyphae are filamentous
structures made of thin, transparent, tubular walls filled or lined with a layer
of protoplasm. Hyphae of Oomycota do not have septa and are called
coenocytic or aseptate. Oomycota produce spores both asexually and sexually. Oomycota produce sporangium, a saclike structure, on the somatic
hyphae. Entire contents of sporangium are converted into motile zoospores.

Zoospores bear two dissimilar flagella, one with mastigonemes.
Sexual reproduction in Oomycota results in the union of two different
nuclei. The process of sexual reproduction consists of three distinct phases:
1. PlasmogamyThe two protoplasts form a union, bringing their nuclei close together within the same cell.
2. KaryogamyThe two nuclei fuse.
3. MeiosisNuclear fusion is followed by meiosis (reduction division).
Meiosis reduces the number of chromosomes to haploid. The sex organs of
fungi are called gametangia. The male gametangium is called antheridium
and the female gametangium is called oogonium.
The oogonium contains a multinucleate oosphere surrounded by a layer
of periplasm. The antheridium is small and club shaped. During reproduction, the antheridium comes into contact with the oogonium, develops a fertilization tube, and penetrates the oogonial wall and the periplasm. The male
nucleus passes through the tube into the oosphere, unites with the female
nucleus, and forms a zygote. The zygote is transformed into an oospore by
the development of a thick cell wall. Oospores are resting spores that germinate by producing a germ tube. Germ tubes may terminate in a sporangium
(as in the case of Phytophthora and Plasmopara) or the oospores may germinate producing a vesicle (as in the case of Pythium and Albugo). Zoospores are formed in the sporangium or in the vesicle by meiosisthe division of oospore contents into a large number of uninucleate sections. Each
of the sections becomes a biflagellate zoospore.
In some Oomycota (as in the case of Phytophthora infestans), sexual
reproduction can occur between the oogonium and antheridium from two
different, sexually compatible mycelia (thalli). This condition is called heterothallism. In many other cases (as in the case of Pythium), sexual reproduction occurs between the oogonium and antheridium from the same
thallus. This condition is called homothallism.
PROTOZOA
Fungal Phyla in Protozoa
The Kingdom Protozoa includes four fungal phyla: Acrasomycota, Dictyosteliomycota, Myxomycota, and Plasmodiophoromycota. Only Plasmodiophoromycota contains important crop pathogens. In this phylum, the order
Plasmodiophorales contains the family Plasmodiophoraceae, in which two

important crop pathogens are included: Plasmodiophora brassicae, which
causes clubroot in various cruciferous plants (including cabbage), and
Spongospora subterranea, which causes powdery scab disease in potatoes.
Structure of Plasmodiophoromycota
The important character of Plasmodiophoromycota is the presence of
plasmodium. Plasmodium is a naked (wall-less) motile mass of protoplasm
with many nuclei that is bounded by a plasma membrane. Plasmodium
moves and feeds in amoeboid fashion (i.e., it engulfs food and feeds by ingestion). Plasmodiophoromycota produce resting spores that germinate and
produce zoospores. Zoospores swim in water droplets, encyst, penetrate the
host tissue, and develop into multinucleate plasmodium. The plasmodium is
cleaved into multinucleate portions. Each portion is surrounded by a membrane and develops into zoosporangium. Zoospores are released outside the
host and can infect the host. Instead of developing into zoosporangia, some
plasmodia produce resting spores that are formed by the formation and copulation of gametes. Plasmogamy, karyogamy, and meiosis may follow and
resting spores are formed.
PHYLA IN THE KINGDOM FUNGI
The Kingdom Fungi encompasses large numbers of fungi. It consists of
four phyla, 103 orders, 484 families, and 4,970 genera (Hawksworth et al.,
1995). Several authors have tried to classify fungi. Barr (1992) and Hawksworth et al. (1995) divided Fungi into Ascomycota, Basidiomycota, Chytridiomycota, and Zygomycota. However, Barr (1992) called the Kingdom
Fungi as Eumycota. It is now accepted by FungalWeb (n.d.) that the Kingdom Fungi should have four phyla (divisions): Ascomycota, Basidiomycota, Chytridiomycota, and Zygomycota. FungalWeb was created by an international group of mycologists who met in Copenhagen in October 1999.
The Web site <http://www.fungalweb.com> provides a classification for all
fungal genera based on a combination of phenotypic and genotypic data.
Another group of fungi is called Mitosporic fungi. It was previously recognized as Deuteromycetes or Deuteromycotina, or Fungi Imperfecti. Mitosporic fungi are an artificial assemblage of fungi that have not been correlated with any meiotic states.
ASCOMYCOTA
Structure of Ascomycota
Ascomycota are either single-celled (yeasts), filamentous (hyphal), or
both (dimorphic). Yeasts (order: Saccharomycetales) grow by budding or
fission. Hyphae of other Ascomycota (Euascomycetes) grow apically and
branch laterally. Yeasts are not important plant pathogens. Only Galactomyces, Geotrichum, Saccharomyces, and Zygosaccharomyces are known to
cause some minor disorders in crop plants. The Euascomycetes are characterized by septate mycelium. The cross-walls that divide the hypha into cells
are called septa. During certain stages of fungal development, the mycelium
becomes organized into loosely or compactly woven tissues, as distinguished from the loose hyphae ordinarily composing a thallus. The loosely
woven tissue in which component hyphae lie more or less parallel to one another is called prosenchyma. The compact woven fungal tissue consists of
closely packed, more or less isodiametric or oval cells resembling the parenchyma cells of higher plants, and this type of fungal tissue is called
pseudoparenchyma. Both prosenchyma and pseudoparenchyma compose
various types of vegetative (somatic) and reproductive structures of Ascomycota.
Stroma is usually made up of prosenchyma, whereas sclerotium is made
up of pseudoparenchymatous tissue. Both stromata and sclerotia are somatic structures of fungi. Stroma is a compact somatic structure that looks
like a mattress. On or in the stroma, fructifications (the structures containing
spores) are formed. Sclerotium is a hard and compact vegetative resting
structure that is resistant to unfavorable conditions. Fungi may overwinter
in the form of sclerotia. The mycelium of some fungi forms thick strands. In
such strands, the hyphae lose their individuality and form complex tissues.
The strands are called rhizomorph. The strands have a thick, hard cortex and
a growing tip that looks like a root tip.
Asexual Reproduction in Ascomycota
Ascomycota reproduce both sexually and asexually. Asexual reproduction is repeated several times in the life cycle of Ascomycota, whereas sexual reproduction occurs only once per life cycle. Different types of asexual
reproduction have been reported in Ascomycota. The fungi may multiply by
fragmentation of hyphae. By this method, the hyphae break up into their
component cells, which behave like spores. These spores are called oidia or
arthrospores. When the cells become enveloped in a thick wall before they
separate from one another or from other hyphal cells adjoining them, they
are called chlamydospores.
Yeasts reproduce by fission or budding. During fission, the cell is split
into daughter cells by constriction and formation of a cell wall. In the budding process, a small outgrowth (bud) is produced from a parent cell. The
nucleus of the parent cell divides and one daughter nucleus migrates into the
bud. The bud increases in size and ultimately breaks off, forming a new individual.
Most Ascomycota reproduce by producing conidia. Conidia are produced at the tips or sides of hyphae. The specialized hypha, which bears
conidia, is called a conidiophore. Conidia are generally borne on conidiophores, which may be produced loosely and indiscriminately by the somatic
hyphae or grouped in various types of asexual fruiting bodies. Conidiophores may be simple or branched. They may look like somatic hyphae or
they may be provided with sterigmata (small hyphal branches that support
the conidia) or specialized branches on which they bear conidia.
Conidiophores may be organized into definite fruiting bodies in the case
of some fungi. The various fruiting bodies reported in Ascomycota include:
1. PycnidiumA hollow, globose, or flask-shaped structure whose pseudoparenchymatous walls are lined with conidiophores.
2. AcervulusAn aggregation (mat) of hyphae (pseudoparenchyma) that
is subcuticular, epidermal, or deeper in origin and never entirely superficial. From the mat, short, closely-packed conidiophores arise,
forming a bedlike mass.
3. SporodochiumA cushion-shaped stroma covered with short conidiophores that are cemented together. The spore mass is supported by
these conidiophores.
4. Pionnote sporodochiumA minute sporodochium near the surface of
the substratum having no stroma. The spores form a continuous slimy
layer.
5. SynnemaA more or less compacted group of erect and sometimes
fused conidiophores bearing conidia at the apex only or on both the
apex and sides. In synnema, conidiophores may be cemented together
to form the elongated, spore-bearing structure. This structure may be
split in different ways near the apex, sometimes resembling a feather
duster.
Sexual Reproduction in Ascomycota
In most species in Ascomycota, the sex organs are ascogonium (the female gametangium) and antheridium (the male gametangium). The male
nucleus passes from the antheridium into the ascogonium through a pore developed at the point of contact between the two gametangia. The ascogonium contains a slender structure called the trichogyne, which receives
the male nucleus. Male nuclei enter the ascogonium and, in many cases, pair
with ascogonial nucleus (plasmogamy). Fusion of nuclei (karyokamy) does
not take place immediately. The ascogonium produces a number of papillae.
The nuclei from the ascogonium begin to pass into these papillae one by
one. The papillae elongate into ascogenous hyphae. In this ascogenous
hyphae, a leading pair of nuclei appear, followed by a second pair.
The nuclei in the ascogenous hyphae and those still in the ascogonium
undergo simultaneous mitosis. Septa are formed and the tip cell of the
ascogenous hypha becomes uninucleate. Several other cells become binucleate. One nucleus in each binucleate cell of the ascogenous hyphae is
antheridial in origin while the other is ascogonial. One of the binucleate
cells of the ascogenous hypha elongates and bends over to form a crozier
(hook). The two nuclei in this hooked cell divide in such a way that their
spindles are oriented more or less vertically and parallel to each other, so
that two of the daughter nucleione from each spindle and, therefore, of
different originare close to each other at the end of the hook, while one of
the other two nuclei is located at the tip and one near the basal septum of the
hook. Two septa form, separating the hook into three cells. The tip and basal
cells are uninucleate, one containing an antheridial nucleus and one an ascogonial nucleus. The crook (hooked) cell is binucleate. The crook cell becomes the ascus and is called the ascus mother cell.
Karyokamy takes place in the ascus mother cell soon after the septa are
formed in the hook. The young ascus with its diploid zygote nucleus begins
to elongate. The zygote nucleus soon undergoes meiosis, resulting in four
haploid nuclei. The nuclei divide mitotically to form eight nuclei. Each nucleus is enveloped by a wall and eight ascospores are formed. Each ascogenous hypha branches and rebranches in various ways and produces a cluster of asci. The saclike structure, which contains the ascospores, is called the
asci.
In most of Ascomycota, the asci are elongated and either club shaped or
cylindrical. The ascus has a single cavity in which the ascospores are
formed. Asci may be stalked or sessile. A definite layer of asci, whether naked or enclosed in a fruiting body, is called the hymenium. Sterile, elongated
hairs called paraphyses are found between the asci in the hymenium. Asci
are categorized based on the structure of their wallsunitunicate or bitunicate. The wall of a unitunicate ascus consists of two thin layers. In the
bitunicate ascus, two distinct wall layers exist: a rigid outer and an extensible inner wall.
With a few exceptions, Ascomycota produce their asci in fruiting bodies

called ascocarps. A few Ascomycota produce naked asci without any fruiting body. Different kinds of ascocarps have been reported:
1. CleistotheciumA completely closed ascocarp
2. PeritheciumA more or less closed ascocarp. At maturity, the perithecium is provided with a pore through which the ascospores escape
3. ApotheciumAn open ascocarp
4. PseudotheciumAn ascostromatic ascocarp in which asci are formed
in numerous unwalled locules (cavities) within the stroma. The stroma
itself forms the wall of the ascocarp.
In some Ascomycota, no antheridia are formed. However, nuclei reach
ascogonia by means of spermatia, microconidia, or conidia. Spermatia are
minute, sphaerical or rod-shaped, uninucleate, male sex cells that become
attached to the receptive organs (trichogynes or somatic hyphae) and empty
their contents into them. Spermatia are detached from the spermatiophore
(parent hyphae) and are carried by wind, water, or insects to receptive organs. Microconidia are minute conidia that behave as spermatia, but are capable of germinating and giving rise to mycelium. Conidia and oidia may
also function as spermatia. In some Ascomycota, fusion of somatic hyphae
of two compatible mycelia takes place, and the nuclei migrate to the
ascogonia through septal perforations.
Classification of Ascomycota
There is considerable variation between the classification systems proposed by several authors (Hawksworth et al., 1995). There is no consensus
about categories above order in the classification. According to Hawksworth et al. (1995), the following 46 orders have been recognized in Ascomycota: Arthoniales, Caliciales, Calosphaeriales, Coryneliales, Cyttariales,
Diaporthales, Diatrypales, Dothideales, Elaphomycetales, Erysiphales, Eurotiales, Gyalectales, Halosphaeriales, Hypocreales, Laboulbeniales, Lahmiales, Lecanorales, Leotiales, Lichinales, Medeolariales, Meliolales, Microascales, Neolectales, Onygenales, Ophiostomatales, Ostropales, Patellariales,
Peltigerales, Pertusariales, Pezizales, Phyllachorales, Pneumocystidales,
Protomycetales, Pyrenulales, Rhytismatales, Saccharomycetales, Schizosaccharomycetales, Sordariales, Spathulosporales, Taphrinales, Teloschistales, Triblidiales, Trichosphaeriales, Trichotheliales, Verrucariales, and Xylariales. Among these orders, only a few contain crop pathogens. Important
crop pathogens belong to the following orders:
Order Diaporthales, Family Valsaceae: Diaporthe, Gnomonia,

Endothia, Phoma, Valsa
Order Dothideales, Family Capnodiaceae: Capnodium
Order Dothideales, Family Elsinoaceae: Elsinoe
Order Dothideales, Family Leptosphaeriaceae: Leptosphaeria,
Ophiobolus
Order Dothideales, Family Lophiostomataceae: Trichometasphaeria
Order Dothideales, Family Mycosphaerellaceae: Mycosphaerella,
Guignardia, Sphaerulina
Order Dothideales, Family Myriangiaceae: Myriangium
Order Dothideales, Family Phaeosphaeriaceae: Phaeosphaeria
Order Dothideales, Family Pleosporaceae: Pleospora, Pyrenophora,
Cochliobolus, Leptosphaerulina, Setosphaeria
Order Dothideales, Family Venturiaceae: Venturia
Order Dothideales, Genera Incertae Sedis: Didymella
Order Erysiphales, Family Erysiphaceae: Blumeria, Sphaerotheca,
Erysiphe, Uncinula, Leveillula, Phyllactinia, Podosphaera
Order Eurotiales, Family Trichocomaceae: Emericella, Eurotium,
Talaromyces
Order Hypocreales, Family Clavicipitaceae: Balansia, Claviceps
Order Hypocreales, Family Hypocreaceae: Nectria, Calonectria,
Gibberella
Order Microascales, Familia Incertae Sedis: Ceratocystis
Order Microascales, Family Microascaceae: Microascus
Order Ophiostomatales, Family Ophiostomataceae: Ophiostoma
Order Phyllachorales, Family Phyllachoraceae: Phyllachora,
Glomerella, Polystigma
Order Protomycetales, Family Protomycetaceae: Protomyces
Order Sordariales, Family Ceratostomataceae: Arxiomyces (=
Ceratostoma), Scopinella (= Ophiostomella)
Order Taphrinales, Family Taphrinaceae: Taphrina
Order Xylariales, Family Xylariaceae: Hypoxylon, Xylaria,
Ustulina, Rosellinia
Ascomycota, Familia Incertae Sedis:
Familia Incertae SedisHyponectriaceae: Physalospora,
Hyponectria, Monographella
Familia Incertae SedisMagnaporthaceae: Gaeumannomyces,
Magnaporthe
Familia Incertae SedisPhlyctidaceae: Phlyctis
Ascomycota, Familia Incertae Sedis, Genera Incertae Sedis:
Leptosphaerella
According to FungalWeb, the phylum Ascomycota consists of three

subphyla (Pezizomycotina, Saccharomycotina, and Taphrinomycotina), several uncertain orders (Ascomycota order uncertain), uncertain families
(Ascomycota families uncertain), and uncertain genera (Ascomycota genera uncertain). The subphylum Pezizomycotina consists of several classes
such as Arthoniomycetes, Chaetothyriomycetes, Dothideomycetes, Dothideomycetes uncertain, Eurotiomycetes, Lecanoromycetes, Lecanoromycetes uncertain, Leotiomycetes, Pezizomycetes, Sordariomycetes, and Dothidiomycetes et Chaetothyriomycetes uncertain. Several Sordariomycetes
orders uncertain, Sordariomycetes families uncertain, and Sordariomycetes
genera uncertain are also included under Pezizomycotina. The class Dothideomycetes contains three orders, Dothideales, Patellariales, and Pleosporales,
and the important families in these orders containing crop pathogens are
given here:
Order Dothideales, Family Dothideaceae: Dothidia
Order Pleosporales, Family Leptosphaeriaceae: Leptosphaeria,
Ophiobolus
Order Pleosporales, Family Lophiostomataceae:
Trichometasphaeria
Order Pleosporales, Family Pleosporaceae: Cochliobolus,
Pseudocochliobolus, Pleospora, Macrospora, Pyrenophora,
Leptosphaerulina
The class Dothideomycetes et Chaetothyriomycetes uncertain contains some
important pathogens:
Family Botryosphaeriaceae: Botyosphaeria
Family Elsinoaceae: Elsinoe
Family Mycosphaerellaceae: Mycosphaerella, Guignardia
Family Venturiaceae: Venturia
Dothideomycetes uncertain includes the crop pathogens Didymella and
Diapleela.
The class Leotiomycetes consists of the following important powdery
mildew fungi and other important pathogens:
Order Erysiphales, Family Erysiphaceae: Erysiphe, Uncinula,
Sphaerotheca, Blumeria, Leveillula, Phyllactinia, Podosphaera,
Microsphaera (powdery mildew fungi)
Order Leotiales, Family Dermateaceae: Diplocarpon, Tapesia
Order Leotiales, Family Sclerotiniaceae: Botryotinia, Sclerotinia
The class Sordariomycetes contains many crop pathogens as follows:

Order Diaporthales, Family Melanconidaceae: Schizoparme,
Sydowiella
Order Diaporthales, Family Valsaceae: Diaporthe, Endothia,
Gnomonia, Valsa
Order Diaporthales Uncertain: Cryptonectria
Order Hypocreales, Family Bionectriaceae: Nectriella
Order Hypocreales, Family Clavicipitaceae: Balansia, Claviceps
Order Hypocreales, Family Nectriaceae: Nectria, Gibberella,
Calonectria
Order Microascales, Family Microascaceae Uncertain: Ceratocystis
Order Ophiostomatales, Family Ophiostomataceae: Ophiostoma
Order Sordariales, Family Ceratostomataceae: Ceratostoma
Order Sordariales Uncertain: Monosporascus
Order Xylariales, Family Clypeosphaeriaceae: Ceratostomella
Order Xylariales, Family Diatrypaceae: Eutypa
Order Xylariales, Family Hypoxylon: Rosellinia, Xylaria,
Hypoxylon
Class Sordariomycetes Orders Uncertain, Order Meliolales, Family
Meliolaceae: Meliola
Class Sordariomycetes Orders Uncertain, Order Phyllachorales,
Family Phyllachoraceae: Phyllachora
Order Sordariomycetes Families Uncertain, Family
Hyponectriaceae: Hyponectria, Physalospora, Monographella
Order Sordariomycetes Families Uncertain, Family
Magnaporthaceae: Magnaporthe, Gaeumannomyces
Order Sordariomycetes Genera Uncertain: Glomerella
BASIDIOMYCOTA
Structure and Reproduction in Basidiomycota
The phylum Basidiomycota consists of septate mycelium. The mycelium
of most Basidiomycota passes through three distinct stages of development,
the primary, the secondary, and the tertiary, before the fungus completes its
life cycle. The basidiospore germinates and grows into the primary mycelium, which is multinucleate in the initial stage. Septa develop and the primary mycelium becomes septate and uninucleate.
The secondary mycelium originates from the primary mycelium. Its cells
are binucleate. Protoplasts of two uninucleate cells fuse without actual
karyogamy and binucleate cells develop (plasmogamy). The binucleate cell
thus formed produces a branch into which the nuclear pair migrates. The
two nuclei divide conjugately and the sister nuclei separate into two daughter cells, thus initiating the binucleate mycelium. In some fungi, the daughter cell formation occurs through clamp connection. Clamp connection is a
bridgelike connection in the secondary mycelium. The binucleate mycelium gives rise to the basidium in which karyogamy and meiosis occur. It is
similar to the ascogenous hyphae from which asci arise.
The tertiary mycelium is an organized tissue that composes the sporophores. The cells of the tertiary mycelium are binucleate, with the sporophores actually originating when the secondary mycelium forms complex
tissues.
The basidium, dikaryotic mycelium, and the formation of clamp connections are characteristics of Basidiomycota. In addition, septa of secondary
mycelium of some Basidiomycota are dolipore septa. This septum flares up
in the middle portion of the hypha, forming a barrel-shaped structure with
open ends.
The sexual structure of Basidiomycota is the basidium. The basidium
originates as a terminal cell of a binucleate hypha and is separated from the
rest of the hypha by a septum over which a clamp connection is generally
found. The basidium soon enlarges and becomes broader. The two nuclei
within the young basidium fuse (karyogamy). The zygote nucleus soon undergoes meiosis, giving rise to four haploid nuclei. Four sterigmata arise at
the top of the basidium and their tips enlarge, forming the basidiospore initials. The four nuclei move through the sterigmata into the young basidiospores. Uninucleate basidiospores develop subsequently. Some Basidiomycota produce their basidia in highly organized fruiting bodies that are
called basidiocarps. However, the fungi causing rust and smut diseases do
not form any basidiocarps.
Asexual reproduction in the Basidiomycota takes place by means of budding, by fragmentation of the mycelium, and by the production of conidia,
arthrospores, or oidia. The rusts produce urediniospores, which are conidial
in origin and function. Smuts produce chlamydospores.
Classification of Basidiomycota
Hawksworth et al. (1995) recognized three classes in the phylum Basidiomycota: Basidiomycetes, Teliomycetes, and Ustomycetes. The class Basidiomycetes consists of 32 orders. The important orders, which consist of crop
pathogens, are Agaricales, Atractiellales, Boletales, Cantharellales, Ceratobasidiales, Ganodermatales, Hymenochaetales, Lachnocladiales, Poriales,
Schizophyllales, and Stereales. The important plant-pathogenic genera included in these orders are given here:
Order Agaricales, Family CoprinaceaeCoprinus
Order Agaricales, Family StrophariaceaePsilocybe
Order Agaricales, Family TricholomataceaeArmillaria,
Armillariella, Baeospora, Clitocybe, Collybia, Crinipellis,
Flammulina, Marasmiellus, Marasmius, Mycena
Order Atractiellales, Family ChionosphaeraceaeStilbum
Order Boletales, Family ConiophoraceaeSerpula
Order Cantharellales, Family TyphulaceaeTyphula
Order Ceratobasidiales, Family Ceratobasidiaceae
Ceratobasidium, Oncobasidium, Thanatephorus
Order Ganodermatales, Family GanodermateaceaeGanoderma
Order Hymenochaetales, Family HymenochaetaceaeFomitiporia,
Hymenochaete, Phellinus
Order Lachnocladiales, Family LachnocladiaceaeScytinostroma
Order Poriales, Family CoriolaceaeCoriolus, Fomes, Fomitella,
Fomitopsis, Gloeophyllum, Heterobasidion, Hexagonia,
Hirschioporus, Laetiporus, Leptoporus, Oxyporus, Pycnoporus,
Perenniporia, Phaeolus, Pseudophaeolus, Pycnoporus,
Rigidoporus, Trichaptum, Trametes
Order Schizophyllales, Family SchizophyllaceaeSchizophyllum,
Solenia
Order Stereales, Family AleurodiscaceaeAleurodiscus
Order Stereales, Family AtheliaceaeAthelia, Butlerelfia
Order Stereales, Family BotryobasidiaceaeWaitea
Order Stereales, Family CorticiaceaeCorticium, Hypochnus,
Pellicularia
Order Stereales, Family PeniophoraceaePeniophora
Order Stereales, Family SistrotremataceaeTrechispora
Order Stereales, Family SteccherinaceaeSteccherinum, Irpex
Order Stereales, Family StereaceaeStereum
In the class Teliomycetes, many rust fungi are included. The important
pathogens in this class include:
Order Septobasidiales, Family SeptobasidiaceaeSeptobasidium,
Uredinella
Order Uredinales, Family ColeosporiaceaeColeosporium

Order Uredinales, Family CronartiaceaeCronartium
Order Uredinales, Family MelampsoraceaeMelampsora
Order Uredinales, Family MikronegeriaceaeMikronegeria
Order Uredinales, Family PhakopsoraceaePhakopsora,
Cerotelium, Dasturella, Monosporidium, Phragmidiella,
Uredopeltis, Uredostilbe, Angiopsora
Order Uredinales, Family PhragmidiaceaePhragmidium,
Trachyspora, Frommea
Order Uredinales, Family PileolariaceaeUromycladium
Order Uredinales, Family PucciniaceaePuccinia, Uromyces,
Gymnosporangium, Chrysella, Chrysopsora, Peridiopsora,
Marvalia, Stereostratum
Order Uredinales, Family PucciniastraceaePucciniastrum,
Hyalopsora, Melampsorella, Melampsoridium, Uredinopsis,
Melampsora
Order Uredinales, Family PucciniosiraceaePucciniosira,
Trichopsora
Order Uredinales, Family RaveneliaceaeRavenelia, Cystomyces,
Haploravenelia
Order Uredinales, Family Sphaerophragmiaceae
Sphaerophragmium
Order Uredinales, Family UropyxidaceaeDidymopsorella,
Tranzschelia, Uropyxis
Order Uredinales, Familia Incertae SedisAecidium, Blastospora,
Cystopsora, Uredo, Hemileia, Physopella
The class Ustomycetes consists of the orders Exobasidiales, Graphiolales, Platygloeales, Sporidiales, and Ustilaginales, each of which contain
crop pathogens. The important crop pathogens in these orders include the
following:
Order Exobasidiales, Family ExobasidiaceaeExobasidium
Order Graphiolales, Family GraphiolaceaeGraphiola,
Trichodesmium
Order Platygloeales, Family PlatygloeaceaCystobasidium,
Helicobasidium, Platycarpa
Order Sporidiales, Family SporidiobolaceaeLeucosporidium
Order Ustilaginales, Family TilletiaceaeEntyloma, Burrillia,
Neovossia, Urocystis, Tilletia, Tilletiella
Order Ustilaginales, Family UstilaginaceaeUstilago, Planetella,
Sorosporium, Sporisorium, Sphacelotheca, Tolyposporidium,
Tranzscheliella, Ustacystis, Pericladium
According to FungalWeb (n.d.), the phylum Basidiomycota consists of

three classes (Hymenomycetes, Teliomycetes, and Ustilaginomycetes) and
several families uncertain and genera uncertain. The class Hymenomycetes
contains the orders Agaricales, Polyporales, Septobasidiales s.l. (s.l. stands
for sensu lato, in the broad sense), Tremellales, Tulasnellales, and Thelephorales. The important pathogens in this class are:
Order Agaricales, family MarasmiaceaeArmillaria
Order Polyporales, Family FomitaceaeFomes
Order Polyporales, Family GanodermataceaeGanoderma
Order Septobasidiales s.l., Family Cystobasidiaceae
Cystobasidium
Order Tulasnellales, Family CeratobasidiaceaeCeratobasidium
In the class Teliomycetes, three orders consist of important crop pathogens:
Order Pucciniales, Family ColeosporiaceaeColeosporium
Order Pucciniales, Family CronartiaceaeCronartium
Order Pucciniales, Family MelamsporaceaeMelampsora
Order Pucciniales, Family PhakopsoraceaeCerotelium,
Phakopsora
Order Pucciniales, Family PhragmidiaceaePhragmidium
Order Pucciniales, Family PucciniaceaePuccinia, Uromyces,
Gymnosporangium
Order Pucciniales, Family PucciniastraceaeMelampsorella,
Melampsoridium
Order Pucciniales, Family RaveneliaceaeRavenelia
Order Pucciniales, Family Sphaerophragmiaceae
Sphaerophragmium
Order Pucciniales, Family UropyxidaceaeTranzschelia
Order Septobasidiales s.str. (s.str. stands for sensu stricto = in the
strict sense), Family SeptobasidiaceaeSeptobasidium,
Uredinella
The class Ustilaginomycetes consists of the orders Entylomatales, Exobasidiales, Tilletiales, Urocystales, Ustilaginales, and Microstromatales,
each of which include crop pathogens. The important crop pathogens in this
class are provided here:
Order Entylomatales, Family EntylomataceaeEntyloma
Order Exobasidiales, Family ExobasidiaceaeExobasidium,
Graphiola
Order Tilletiales, Family TilletiaceaeTilletia, Neovossia

Order Urocystales, Family UrocystaceaeUrocystis
Order Ustilaginales, Family UstilaginaceaeUstilago,
Tolyposporium
In the Basidiomycota families uncertain, one important crop pathogen,
Corticium is included in the family Corticiaceae. In the Basidiomycota genera uncertain, the crop pathogen Hemileia is included.
CHYTRIDIOMYCOTA
The thallus of Chytridiomycota can be coenocytic, holocarpic (i.e., the
thallus is entirely converted into one or more reproductive structures),
eucarpic (i.e., reproductive structures form on certain portions of the thallus,
which continues to perform its somatic functions), monocentric (i.e., the
thallus radiates from a single point at which a reproductive organ such as the
sporangium is formed), polycentric (i.e., the thallus radiates from many
centers at which reproductive organs are formed), or mycelial. At least in
hyphal stages, the cell wall consists of chitin. The zoospores are monoflagellate, lacking mastigonemes or scales. An important crop pathogen belonging to the phylum Chytridiomycota is Synchytrium endobioticum, which
causes wart disease in potato. It possesses uniflagellate zoospores. Zoospores swim in soil moisture and penetrate the epidermal cells of host tissues, shedding their flagellum outside. The unicellular fungus grows in size
and the nucleus becomes greatly enlarged. After reaching a certain size, it
secretes a thick, golden-brown wall. The well-developed structure is called
prosorus. The prosorus germinates within the host cell. The cell wall of
prosorus ruptures and the proplast is surrounded by a thin hyaline membrane. Repeated mitotic divisions of the nucleus take place, forming about
32 nuclei. Thin hyaline walls are formed in such a way as to divide the
prosorus into four to nine multinucleate segments. Nuclear division continues and about 300 nuclei are formed. Each prosorus portion develops into a
sporangium or a gametangium, depending on environment. The mass of
sporangia is called the sorus. If water is abundantly present, zoospores are
formed. If drought sets in, the motile cells that are released become planogametes (motile gametes) instead of zoospores. The planogametes copulate
in pairs (fusion of naked gametes takes place) resulting in the formation of
biflagellate zygotes. The zygotes swim in soil moisture until they penetrate
epidermal host cells. Inside each host cell the zygote elongates and a heavy
wall is formed around it. It is now called resting sporangium. From the resting sporangium zoospores are formed.
The phylum Chytridiomycota consists of five orders: Blastocladiales,

Chytridiales, Monoblepharidales, Neocallimastigales, and Spizellomycetales
(Hawksworth et al., 1995). The plant pathogens of Chytridiomycota include:
Order Blastocladiales, Family PhysodermateaceaePhysoderma
Order Chytridiales, Family ChytridiaceaePhlyctidium
Order Chytridiales, Family SynchytriaceaeSynchytrium
Order Spizellomycetales, Family OlpidiaceaeOlpidium
Order Spizellomycetales, Family Spizellomycetaceae
Rhizophlyctis
Order Spizellomycetales, Family UrophlyctidaceaeUrophlyctis
ZYGOMYCOTA
The mycelium of Zygomycota is coenocytic (aseptate). The important
character of this phylum is the production of zygospores. A zygospore typically results from the fusion of two gamentangia. Zygospores differ from
the oospores of Oomycota in that the latter are derived from an oosphere.
Asexual reproduction in this phylum is by means of nonmotile (aplanate)
spores. Zygomycota includes only a few weak pathogens such as Rhizopus
and Mucor.
The mycelium of Rhizopus develops rhizoids (a short branch of a thallus
that looks like a root) at certain points of the mycelium. Directly above the
rhizoids, sporangiophores are produced. The top of the sporangiophores
becomes swollen and a sporangium develops. The central portion of the
sporangium is a columella (a sterile structure that is an extension of the
stalk). The peripheral zone is the spore-bearing portion of the sporangium.
Sporangiospores (aplanospores = nonmotile spores) are produced inside the
sporangium. Rhizopus is heterothallic (i.e., two different thalli are required
for sexual reproduction). When two opposite strains (+ and -) come in contact with each another, progamentangia (before gamentagium) are formed.
A septum forms near the tip of each progamentagium, separating into two
cells (a terminal gamentagium and a suspensor cell). The walls of the two contacting gamentagia dissolve at the point of contact and the two protoplasts
mix. Fusion of nuclei takes place. The cell wall of the fused gametangia
thickens and the resulting structure is called a zygospore. A sporangiophore
arises and develops into a sporangium, which is called a zoosporangium.
Aplanospores develop in the zoosporangium and are released into the ambient water droplets (Vidhyasekaran, 1993).
The phylum Zygomycota consists of two classes, Trichomycetes and
Zygomycetes (Hawksworth et al., 1995). Zygomycetes contains plant patho-
gens and consists of seven orders: Dimargaritales, Endogonales, Entomophthorales, Glomales, Kickxellales, Mucorales, and Zoopagales. Mucorales contains crop pathogens, which include:
Order: Mucorales, Family MucoraceaeRhizopus, Mucor
Order: Mucorales, Family CunninghamellaceaeCunninghamella
According to the FungalWeb (n.d.), the order Mucorales does not contain
families.
MITOSPORIC FUNGI
Mitosporic fungi is an artificial assemblage of fungi in which sexually
produced spores such as ascospores or basidiospores are absent or presumably absent. Mitosporic fungi produce conidia that are formed by mitosis.
Mitosporic fungi appears to lack a teleomorph (sexual stage [form]) and has
only an anamorph (asexual stage [form]). Mitosporic fungi also include
fungi in which both meiotic (sexual) and mitotic (asexual) reproductive
structures are absent. Mitosporic fungi cannot be assigned to families in the
accepted phyla. However, some of these fungi have been found correlated
with teleomorphs in the Ascomycota and Basidiomycota. Such fungi have
been placed in families of the corresponding Ascomycota and Basidiomycota phyla as Anamorphic families. For example, the asexual spores
(conidia) produced by Botrytis cinerea (a Mitosporic fungi which lacks a
sexual stage) are similar to the asexual spores produced by Botryotinia
fuckeliana (a fungus with both sexual and asexual stages, belonging to the
family Sclerotiniaceae, class Ascomycota). As such, Botrytis cinerea is
classified as Anamorphic Sclerotiniaceae. Numerous crop pathogens belong to Mitosporic fungi. Some important pathogens in this group are listed
here:
Anamorphic AmphisphaeriaceaePestalotiopsis
Anamorphic BotryosphaeriaceaeDiplodia, Fusicoccum
Anamorphic CapnodiaceaeTripospermum
Anamorphic ClavicipitaceaeEphelis
Anamorphic CorticiaceaeRhizoctonia
Anamorphic DermateaceaeCylindrosporium
Anamorphic DothideaceaeSeptoria
Anamorphic DothidealesAscochyta
Anamorphic ElsinoaceaeSphaceloma
Anamorphic ErysiphaceaeOvulariopsis
Anamorphic HypocreaceaeFusarium
Anamorphic MagnaporthaceaePyricularia
Anamorphic MelanconidaceaeMelanconium
Anamorphic MycosphaerellaceaeCercospora, Phyllosticta
Anamorphic PhyllachoraceaeColletotrichum
Anamorphic PleosporaceaeAlternaria, Drechslera, Phoma,
Stemphylium, Exserohilum
Anamorphic SclerotiniaceaeBotrytis
Anamorphic SistrotremataceaePhymatotrichopsis
Anamorphic TrichocomaceaeAspergillus, Penicillium
Anamorphic UredinalesAecidium, Uredo
Anamorphic ValsaceaePhoma, Phomopsis
Anamorphic VenturiaceaeFusicladium
Anamorphic XylariaceaeDematophora
Mitosporic FungiBotryodiplodia, Cephalotrichum,
Cercosporidium, Chalara, Cordana, Corynespora, Fulvia,
Gloeosporium, Helminthosporium, Lasiodiplodia,
Macrophomina, Myrothecium, Pestalotia, Phaeoisariopsis,
Phyllostictella, Phymatotrichum, Rhynchosporium, Sarocladium,
Sclerotium, Thielaviopsis, Trichoconis, Verticillium
SYSTEMATIC POSITIONS OF CROP FUNGAL PATHOGENS
(INCLUDING CHROMISTA AND PROTOZOA)
The systematic positions of all crop fungal pathogens are given here following the classification of Hawksworth et al. (1995) and Wrobel and
Creber (1998). The positions of these fungi according to FungalWeb are
given within parentheses. The names of genera of fungi are also provided.
The names of species of crop pathogens with authority and the names of diseases caused by them are provided in Chapter 30.
AcanthorhynchusHyponectriaceae, Familia Incertae Sedis, Ascomycota
(Hyponectriaceae, Sordariomycetes Families Uncertain, Ascomycota)
AchlyaSaprolegniaceae, Saprolegniales, Oomycota, Chromista
(Saprolegniaceae, Saprolegniales, Oomycetes, Chromista)
AcremoniumMitosporic fungi
AcrocalymmaAnamorphic Lophiostomataceae
AcrocylindriumMitosporic fungi
AcrodontiumMitosporic fungi
AcrophialophoraMitosporic fungi
ActinonemaMitosporic fungi
AecidiumAnamorphic Uredinales (Basidiomycota Genera Uncertain,

Basidiomycota Families Uncertain, Basidiomycota)
AkaropeltopsisMicropeltidaceae, Dothideales, Ascomycota
(Micropeltidaceae, Dothideomycetes et Chaetothyriomycetes Uncertain, Ascomycota)
AlbugoAlbuginaceae, Peronosporales, Oomycota, Chromista
(Albuginaceae, Peronosporales, Oomycetes, Chromista)
AleurodiscusAleurodiscaceae, Stereales, Basidiomycetes,
Basidiomycota (Aleurodiscaceae, Hericiales, Hymenomycetes,
Basidiomycota)
AllantophomopsisMitosporic fungi
AlternariaAnamorphic Pleosporaceae
AngiopsoraPhakopsoraceae, Uredinales, Teliomycetes, Basidiomycota
(Phakopsoraceae, Pucciniales, Teliomycetes, Basidiomycota)
AntennulariaVenturiaceae, Dothideales, Ascomycota (Venturiaceae,
Dothideomycetes et Chaetothyriomycetes Uncertain, Ascomycota)
AphanomycesSaprolegniaceae, Saprolegniales, Oomycota, Chromista
(Saprolegniaceae, Saprolegniales, Oomycetes, Chromista)
ApiognomoniaValsaceae, Diaporthales, Ascomycota (Valsaceae,
Diaporthales, Sordariomycetidae, Sordariomycetes, Ascomycota)
ApiosporaLasiosphaeriaceae, Sordariales, Ascomycota (Apiosporaceae,
Sordariomycetes Families Uncertain, Ascomycota)
ApiosporinaVenturiaceae, Dothideales, Ascomycota (Venturiaceae,
ApostrasseriaAnamorphic Phacidiaceae
ArmillariaTricholomataceae, Agaricales, Basidiomycetes,
Basidiomycota (Marasmiaceae, Agaricales, Hymenomycetes,
Basidiomycota)
ArmillariellaTricholomataceae, Agaricales, Basidiomycetes,
Basidiomycota)
ArthoniaArthoniaceae, Arthoniales, Ascomycota (Arthoniaceae,
Arthoniales, Arthoniomycetes, Pezizomycotina, Ascomycota)
ArthriniumAnamorphic Lasiosphaeriaceae
AscochytaAnamorphic Dothideales, Mycosphaerellaceae,
Leptosphaeriaceae
AspergillusAnamorphic Trichocomaceae
AsperisporiumMitosporic fungi
AsteridiellaMeliolaceae, Meliolales, Ascomycota (Meliolaceae,
Meliolales, Sordariomycetes Orders Uncertain, Ascomycota)
AsteromellaAnamorphic Mycosphaerellaceae
AtheliaAtheliaceae, Stereales, Basidiomycetes, Basidiomycota

(Atheliaceae, Basidiomycota Families Uncertain, Basidiomycota)
AureobasidiumMitosporic fungi
BaeosporaTricholomataceae, Agaricales, Basidiomycetes,
Basidiomycota (Tricholomataceae, Agaricales, Hymenomycetes,
Basidiomycota)
BalansiaClavicipitaceae, Hypocreales, Ascomycota (Clavicipitaceae,
Hypocreales, Hypocreomycetidae, Sordariomycetes, Ascomycota)
BeniowskiaMitosporic fungi
BipolarisAnamorphic Pleosporaceae
BiscogniauxiaXylariaceae, Xylariales, Ascomycota (Xylariaceae,
Xylariales, Xylariomycetidae, Sordariomycetes, Ascomycota)
BitrimonosporaSordariales, Ascomycota (Sordariales,
Sordariomycetidae, Sordariomycetes, Ascomycota)
BlumeriaErysiphaceae, Erysiphales, Ascomycota (Erysiphaceae,
Erysiphales, Leotiomycetes, Ascomycota)
BlumeriellaDermateaceae, Leotiales, Ascomycota (Dermateaceae,
Leotiales, Leotiomycetes, Ascomycota)
BotryodiplodiaMitosporic fungi
BotryosphaeriaBotryosphaeriaceae, Dothideales, Ascomycota
(Botryosphaeriaceae, Dothideomycetes et Chaetothyriomycetes Uncertain, Ascomycota)
BotryotiniaSclerotiniaceae, Leotiales, Ascomycota (Sclerotiniaceae,
BotrytisAnamorphic Sclerotiniaceae
BremiaPeronosporaceae, Peronosporales, Oomycota, Chromista
(Peronosporaceae, Peronosporales, Oomycetes, Chromista)
BriosiaMitosporic fungi
ButlerelfiaAtheliaceae, Stereales, Basidiomycetes, Basidiomycota
(Hyphodermataceae, Hymenochaetales s.l., Hymenomycetes,
Basidiomycota)
ByssochlamysTrichocomaceae, Eurotiales, Ascomycota
(Trichocomaceae, Eurotiales, Eurotiomycetes, Ascomycota)
CalonectriaHypocreaceae, Hypocreales, Ascomycota (Nectriaceae,
CapitorostrumMitosporic fungi
CapnodiumCapnodiaceae, Dothideales, Ascomycota (Capnodiaceae,
CatacaumaPhyllachoraceae, Phyllachorales, Ascomycota
(Phyllachoraceae, Phyllachorales, Sordariomycetes Orders Uncertain,
Ascomycota)
CenangiumLeotiaceae, Leotiales, Ascomycota (Leotiaceae, Leotiales,

Leotiomycetes, Ascomycota)
CentrosporaMitosporic fungi
CephalosporiumMitosporic fungi
CephalotheciumMitosporic fungi
CephalotrichumMitosporic fungi
CeratobasidiumCeratobasidiaceae, Ceratobasidiales, Basidiomycetes,
Basidiomycota (Ceratobasidiaceae, Tulasnellales, Hymenomycetes,
Basidiomycota)
CeratocystisFamilia Incertae Sedis, Microascales, Ascomycota
(Microascaceae Uncertain, Microascales, Hypocreomycetidae,
Sordariomycetes, Ascomycota)
CeratomycesCeratomycetaceae, Laboulbeniales, Ascomycota
(Ceratomycetaceae, Laboulbeniales, Ascomycota Orders Uncertain,
Ascomycota)
CeratophomaMitosporic fungi
CercoseptoriaMitosporic fungi
CercosporaAnamorphic Mycosphaerellaceae
CercosporellaMitosporic fungi
CercosporidiumMitosporic fungi
CeroteliumPhakopsoraceae, Uredinales, Teliomycetes, Basidiomycota
CeuthosporaMitosporic fungi
ChaetosphaeropsisMitosporic fungi
ChalaraMitosporic fungi
ChalaropsisMitosporic fungi
ChoanephoraChoanephoraceae, Mucorales, Zygomycetes, Zygomycota
(Mucorales, Zygomycota)
ChondrostereumMeruliaceae, Stereales, Basidiomycetes,
Basidiomycota (Meruliaceae, Meruliales s.str., Hymenomycetes,
Basidiomycota)
ChrysomyxaColeosporiaceae, Uredinales, Teliomycetes, Basidiomycota
(Coleosporiaceae, Pucciniales, Teliomycetes, Basidiomycota)
CiboriaSclerotiniaceae, Leotiales, Ascomycota (Sclerotiniaceae,
CladosporiumAnamorphic Mycosphaerellaceae
ClasterosporiumAnamorphic Magnaporthaceae
ClathridiumAmphisphaeriaceae, Xylariales, Ascomycota
(Amphisphaeriaceae, Xylariales, Xylariomycetidae, Sordariomycetes,
Ascomycota)
ClavicepsClavicipitaceae, Hypocreales, Ascomycota (Clavicipitaceae,
ClitocybeTricholomataceae, Agaricales, Basidiomycetes,

Basidiomycota)
ClypeoportheValsaceae, Diaporthales, Ascomycota (Valsaceae,
CoccomycesRhytismataceae, Rhytismatales, Ascomycota
(Rhytismataceae, Rhytismatales, Leotiomycetes, Ascomycota)
CoccostromaPhyllachoraceae, Phyllachorales, Ascomycota
(Phyllachoraceae, Phyllachorales, Sordariomyctes Orders Uncertain,
Ascomycota)
CochliobolusPleosporaceae, Dothideales, Ascomycota (Pleosporaceae,
Pleosporales, Dothideomycetes, Ascomycota)
ColeomycesMitosporic fungi
ColeosporiumColeosporiaceae, Uredinales, Teliomycetes, Basidiomycota (Coleosporiaceae, Pucciniales, Teliomycetes, Basidiomycota)
ColletotrichumAnamorphic Phyllachoraceae
CollybiaTricholomataceae, Agaricales, Basidiomycetes, Basidiomycota
(Tricholomataceae, Agaricales, Hymenomycetes, Basidiomycota)
ConiellaAnamorphic Melanconidaceae
ConiotheciumMitosporic fungi
ConiothyriumAnamorphic Leptosphaeriaceae
CoprinusCoprinaceae, Agaricales, Basidiomycetes, Basidiomycota
(Agaricaceae, Agaricales, Hymenomycetes, Basidiomycota)
CordanaMitosporic fungi
CordycepsClavicipitaceae, Hypocreales, Ascomycota (Clavicipitaceae,
CoriolusCoriolaceae, Poriales, Basidiomycetes, Basidiomycota
(Coriolaceae, Polyporales, Hymenomycetes, Basidiomycota)
CorticiumCorticiaceae, Stereales, Basidiomycetes, Basidiomycota
(Corticiaceae, Basidiomycota Families Uncertain, Basidiomycota)
CorynesporaMitosporic fungi
CoryneumAnamorphic Melanconidaceae
CrinipellisTricholomataceae, Agaricales, Basidiomycetes,
Basidiomycota (Marasmiaceae, Tricholomataceae, Agaricales,
Hymenomycetes, Basidiomycota)
CristulariellaAnamorphic Sclerotiniaceae
CronartiumCronartiaceae, Uredinales, Teliomycetes, Basidiomycota
(Cronartiaceae, Pucciniales, Teliomycetes, Basidiomycota)
CryphonectriaValsaceae, Diaporthales, Ascomycota (Valsaceae,
CryptodiaportheValsaceae, Diaporthales, Ascomycota (Valsaceae,
CryptosporaValsaceae, Diaporthales, Ascomycota (Valsaceae,

CryptosporellaValsaceae, Diaporthales, Ascomycota (Valsaceae,
CryptosporiopsisAnamorphic Dermateaceae
Cryptosporium Mitosporic fungi
CunninghamellaCunninghamellaceae, Mucorales, Zygomycetes,
Zygomycota (Mucorales, Zygomycota)
CurvulariaAnamorphic Pleosporaceae
CycloconiumAnamorphic Venturiaceae
CylindrocarponAnamorphic Hypocreaceae
CylindrocladiellaMitosporic fungi
CylindrocladiumAnamorphic Hypocreaceae
CylindrosporiumAnamorphic Dermateaceae
CystopusAlbuginaceae, Peronosporales, Oomycota, Chromista
(Albuginaceae, Peronosporales, Oomycota, Chromista)
CytosporaAnamorphic Valsaceae
CytosporinaMitosporic fungi
DactuliophoraMitosporic fungi
DactyliumMitosporic fungi
DasyscyphaHyaloscyphaceae, Leotiales, Ascomycota
(Hyaloscyphaceae, Leotiales, Leotiomycetes, Ascomycota)
DeightoniellaMitosporic fungi
DematophoraAnamorphic Xylariaceae
DendrophomaMitosporic fungi
DeuterophomaMitosporic fungi
DiacheaStemonitidaceae, Stemonitales, Myxomycota (Didymiaceae,
Physarales, Myxomycetes, Myxomycota)
DiapleelaDothideales, Ascomycota (Dothideomycetes Uncertain)
DiaportheValsaceae, Diaporthales, Ascomycota (Valsaceae,
DibotryonVenturiaceae, Dothideales, Ascomycota (Venturiaceae,
DictyochaetaAnamorphic Lasiosphaeriaceae
DidymellaDothideales Incertae Sedis, Ascomycota (Dothideomycetes
uncertain)
DidymosphaeriaDidymosphaeriaceae, Dothideales, Ascomycota
(Didymosphaeriaceae, Dothideomycetes et Chaetothyriomycetes Uncertain, Ascomycota)
DilophosporaAnamorphic Dothideales
DimeriellaParodiopsidaceae, Dothideales, Ascomycota

(Parodiopsidaceae, Dothideomycetes et Chaetothyriomycetes Uncertain, Ascomycota)
DinemasporiumMitosporic fungi
DiplocarponDermateaceae, Leotiales, Ascomycota (Dermateaceae,
DiplodiaAnamorphic Botryosphaeriaceae
DiplodinaAnamorphic Valsaceae
DiscohainesiaDermateaceae, Leotiales, Ascomycota (Dermateaceae,
DiscostromaAmphisphaeriaceae, Xylariales, Ascomycota
Ascomycota)
DoratomycesMitosporic fungi
DothidellaPolystomellaceae, Dothideales, Ascomycota
(Polystomellaceae, Dothideomycetes et Chaetothyriomycetes Uncertain, Ascomycota)
DothiorellaMitosporic fungi
DrechsleraAnamorphic Pleosporaceae
ElsinoeElsinoaceae, Dothideales, Ascomycota (Elsinoaceae,
EntomosproriumAnamorphic Dermateaceae
EntylomaTilletiaceae, Ustilaginales, Ustomycetes, Basidiomycota
(Entylomataceae, Entylomatales, Ustilaginomycetes, Basidiomycota)
EphelisAnamorphic Clavicipitaceae
EpichloeClavicipitaceae, Hypocreales, Ascomycota (Clavicipitaceae,
EpicoccumMitosporic fungi
ErysipheErysiphaceae, Erysiphales, Ascomycota (Erysiphaceae,
ErythriciumHyphodermateaceae, Stereales, Basidiomycota
(Meruliaceae, Meruliales s.str., Hymenomycetes, Basidiomycota)
EupropodellaDermateaceae, Leotiales, Ascomycota (Dermateaceae,
EutypaDiatrypaceae, Diatrypales, Ascomycota (Diatrypaceae,
ExobasidiumExobasidiaceae, Exobasidiales, Ustomycetes,
Basidiomycota (Exobasidiaceae, Exobasidiales, Ustilaginomycetes,
Basidiomycota)
ExserohilumAnamorphic Pleosporaceae
FabraeaDermateaceae, Leotiales, Ascomycota (Dermateaceae,
FlammulinaTricholomataceae, Agaricales, Basidiomycetes,

Basidiomycota)
FomesCoriolaceae, Poriales, Basidiomycetes, Basidiomycota
(Fomitaceae, Polyporales, Hymenomycetes, Basidiomycota)
FomitellaCoriolaceae, Poriales, Basidiomycetes, Basidiomycota
(Fomitaceae, Polyporales, Hymenomycetes, Basidiomycota)
FomitiporiaHymenochaetaceae, Hymenochaetales, Basidiomycetes,
Basidiomycota (Hymenochaetaceae, Hymenochaetales s.str.,
FomitopsisCoriolaceae, Poriales, Basidiomycetes, Basidiomycota
(Fomitopsidaceae, Polyporales, Hymenomycetes, Basidiomycota)
FrommeaPhragmidiaceae, Uredinales, Teliomycetes, Basidiomycota
(Phragmidiaceae, Pucciniales, Teliomycetes, Basidiomycota)
FulviaMitosporic fungi
FusariumAnamorphic Hypocreaceae
FusicladiumAnamorphic Venturiaceae
FusicoccumAnamorphoic Botryosphaeriaceae
GaeumannomycesMagnaporthaceae, Incertae Sedis, Ascomycota
(Magnaporthaceae, Sordariomycetes Families Uncertain, Ascomycota)
GalactomycesDipodascaceae, Saccharomycetales, Ascomycota
(Dipodascaceae, Saccharomycetales, Saccharomycetes, Ascomycota)
GanodermaGanodermateaceae, Ganodermatales, Basidiomycetes,
Basidiomycota (Ganodermataceae, Polyporales, Hymenomycetes,
Basidiomycota)
Geastrumia Mitosporic fungi
Geotrichum Anamorphic Dipodascaceae
GerlachiaMitosporic fungi
GibberellaHypocreaceae, Hypocreales, Ascomycota (Nectriaceae,
GibellinaPhyllachoraceae, Phyllachorales, Ascomycota
Ascomycota)
GilberetellaGilbertellaceae, Mucorales, Zygomycetes, Zygomycota
GloeocercosporaMitosporic fungi
Gloeodes Mitosporic fungi
GloeophyllumCoriolaceae, Poriales, Basidiomycetes, Basidiomycota
(Fomitopsidaceae, Polyporales, Hymenomycetes, Basidiomycota)
GloeosporiumMitosporic fungi
GlomerellaPhyllachoraceae, Phyllachorales, Ascomycota
(Sordariomycetes Genera Uncertain, Ascomycota)
GnomoniaValsaceae, Diaporthales, Ascomycota (Valsaceae,

GodroniaLeotiaceae, Leotiales, Ascomycota (Leotiaceae, Leotiales,
Leotiomycetes, Ascomycota)
GonatobotrysAnamorphic Ceratostomataceae
GraphiolaGraphiolaceae, Graphiolales, Ustomycetes, Basidiomycota
(Exobasidiaceae, Exobasidiales, Ustilaginomycetes, Basidiomycota)
GraphiumAnamorphic Ophiostomataceae
GreeneriaMitosporic fungi
GriphosphaeriaAmphisphaeriaceae, Xylariales, Ascomycota
Ascomycota)
GrovesiniaSclerotiniaceae, Leotiales, Ascomycota (Sclerotiniaceae,
GuignardiaMycosphaerellaceae, Dothideales, Ascomycota
(Mycosphaerellaceae, Dothideomycetes et Chaetothyriomycetes Uncertain, Ascomycota)
GymnoconiaPhragmidiaceae, Uredinales, Teliomycetes, Basidiomycota
GymnosporangiumPucciniaceae, Uredinales, Teliomycetes,
Basidiomycota (Pucciniaceae, Pucciniales, Teliomycetes,
Basidiomycota)
HainesiaAnamorphic Leotiaceae
HalosphaeriaHalosphaeriaceae, Halosphaeriales, Ascomycota
(Halosphaeriaceae, Halosphaeriales, Hypocreomycetidae,
HansenulaSaccharomycetaceae, Saccharomycetales, Ascomycota
(Saccharomycetaceae, Saccharomycetales, Saccharomycetes,
Saccharomycotina, Ascomycota)
HaplobasidionCoriolaceae, Poriales, Basidiomycetes, Basidiomycota
HelicobasidiumPlatygloeaceae, Platygloeales, Ustomycetes,
Basidiomycota (Platygloeaceae, Septobasidiales s.l., Hymenomycetes,
Basidiomycota)
HelminthosporiumMitosporic fungi
HemileiaIncertae Sedis, Uredinales, Teliomycetes, Basidiomycota
(Basidiomycota Genera Uncertain, Basidiomycota Families Uncertain,
Basidiomycota)
HendersoniaMitosporic fungi
HendersonulaMitosporic fungi
HeterobasidionCoriolaceae, Poriales, Basidiomycetes, Basidiomycota
(Echinodontiaceae, Hericiales, Hymenomycetes, Basidiomycota)
HetrosporiumMitosporic fungi
HexagoniaCoriolaceae, Poriales, Basidiomycetes, Basidiomycota
HirschioporusCoriolaceae, Poriales, Basidiomycetes, Basidiomycota
HormodendrumMitosporic fungi
HyalothyridiumMitosporic fungi
HymenellaMitosporic fungi
HymenochaeteHymenochaetaceae, Hymenochaetales, Basidiomycota
(Hymenochaetaceae, Hymenochaetales s.str., Hymenomycetes,
Basidiomycota)
HymenulaMitosporic fungi
HypochnusArthoniaceae, Arthoniomycetes, Ascomycota
(Arthoniaceae, Arthoniomycetes, Pezizomycotina, Ascomycota)
HypocreaHypocreaceae, Hypocreales, Ascomycota (Hypocreaceae,
HypomycesHypocreaceae, Hypocreales, Ascomycota (Hypocreaceae,
HyponectriaHyponectriaceae, Familia Incertae Sedis, Ascomycota
HypoxylonXylariaceae, Xylariales, Ascomycota (Xylariaceae,
Idriella Mitosporic fungi
IrpexSteccherinaceae, Stereales, Basidiomycetes, Basidiomycota
(Steccherinaceae, Hymenochaetales s.l., Hymenomycetes,
Basidiomycota)
IsariopsisMitosporic fungi
ItersoniliaMitosporic fungi
JohncouchiaAnamorphic Septobasidiaceae
JunghuhniaSteccherinaceae, Stereales, Basidiomycota
(Steccherinaceae, Hymenochaetales s.l. Hymenomycetes,
Basidiomycota)
KabatiellaMitosporic fungi
KalmusiaDothideales, Ascomycota (Dothideomycetes Uncertain,
Pezizomycotina, Ascomycota)
KhuskiaAscomycota Incertae Sedis (Sordariomycetes Genera Uncertain, Pezizomycotina, Ascomycota)
KuehneolaPhragmidiaceae, Uredinales, Teliomycetes, Basidiomycota
KunkeliaUredinales, Incertae Sedis
KutilakesaMitosporic fungi
LaetiporusCoriolaceae, Poriales, Basidiomycetes, Basidiomycota

(Phaeolaceae, Polyporales, Hymenomycetes, Basidiomycota)
LasiodiplodiaMitosporic fungi
LeandriaMitosporic fungi
LepteutypaAmphisphaeriaceae, Xylariales, Ascomycota
Ascomycota)
LeptodontiumMitosporic fungi
LeptoporusCoriolaceae, Poriales, Basidiomycetes, Basidiomycota
LeptosphaeriaLeptosphaeriaceae, Dothideales, Ascomycota
(Leptosphaeriaceae, Pleosporales, Dothideomycetes, Ascomycota)
LeptosphaerulinaPleosporaceae, Dothideales, Ascomycota
(Pleosporaceae, Pleosporales, Dothideomycetes, Ascomycota)
LeptothyriumMitosporic fungi
LeptotrochilaDermateaceae, Leotiales, Ascomycota (Dermateaceae,
LeucocytosporaMitosporic fungi
LeucostomaValsaceae, Diaporthales, Ascomycota (Valsaceae,
Leucothallia- Erysiphaceae, Erysiphales, Ascomycota (Erysiphaceae,
LeveillulaErysiphaceae, Erysiphales, Ascomycota (Erysiphaceae,
LibertellaAnamorphic Diatrypaceae, Xylariaceae
LimacinulaCoccodiniaceae, Dothideales, Ascomycota
(Coccodiniaceae, Dothideales, Dothideomycetes, Ascomycota)
LophodermiumRhytimataceae, Rhytismatales, Ascomycota
(Rhytimataceae, Rhytismatales, Leotiomycetes, Ascomycota)
MacrophomaMitosporic fungi
MacrophominaMitosporic fungi
MacrosporiumMitosporic fungi
MagnaportheMagnaporthaceae, Incertae Sedis, Ascomycota
MarasmiellusTricholomataceae, Agaricales, Basidiomycetes,
Basidiomycota)
MarasmiusTricholomataceae, Agaricales, Basidiomycetes,
Basidiomycota)
MarssoninaAnamorphic Dermateaceae
MassarinaLophiostomataceae, Dothideales, Ascomycota

(Lophiostomataceae, Pleosporales, Dothideomycetes, Ascomycota)
MauginiellaMitosporic fungi
MelampsoraMelampsoraceae, Uredinales, Teliomycetes,
Basidiomycota (Melampsoraceae, Pucciniales, Teliomycetes,
Basidiomycota)
MelampsorellaPucciniastraceae, Uredinales, Teliomycetes,
Basidiomycota (Pucciniastraceae, Pucciniales, Teliomycetes,
Basidiomycota)
MelanconiumAnamorphic Melanconidaceae
MelanosporaCeratostomataceae, Sordariales, Ascomycota
(Ceratostomataceae, Sordariales, Sordariomycetidae, Sordariomycetes,
Ascomycota)
MeliolaMeliolaceae, Meliolales, Ascomycota (Meliolaceae, Meliolales,
Sordariomycetes Orders Uncertain, Ascomycota)
MeriaMitosporic fungi
MicrodochiumAnamorphic Hyponectriaceae
MicronectriellaMycosphaerellaceae, Dothideales, Ascomycota
MicrosphaeraErysiphaceae, Erysiphales, Ascomycota (Erysiphaceae,
MoesziomycesUstilaginaceae, Ustilaginales, Ustomycetes,
Basidiomycota (Ustilaginaceae, Ustilaginales, Ustilaginomycetes,
Basidiomycota)
MoniliaMitosporic fungi
MoniliniaSclerotiniaceae, Leotiales, Ascomycota (Sclerotiniaceae,
MoniliophthoraMitosporic fungi
MonilochaetesMitosporic fungi
MonochaetiaMitosporic fungi
MonographellaHyponectriaceae, Familia Incertae Sedis, Ascomycota
MonosporascusSordariales Incertae Sedis, Ascomycota (Sordariales
Uncertain, Sordariomycetidae, Sordariomycetes, Ascomycota)
MucorMucoraceae, Mucorales, Zygomycetes, Zycomycota (Mucorales,
Zygomycota)
MyceliophthoraAnamorphic Arthrodermataceae
MycenaTricholomataceae, Agaricales, Basidiomycetes, Basidiomycota
(Favolaschiaceae, Agaricales, Hymenomycetes, Basidiomycota)
MycocentrosporaMitosporic fungi
MycoleptodiscusAnamorphic Magnaporthaceae
MycosphaerellaMycosphaerellaceae, Dothideales, Ascomycota
Mycovellosiella Mitosporic fungi
MyriogenosporaClavicipitaceae, Hypocreales, Ascomycota
(Clavicipitaceae, Hypocreales, Hypocreomycetidae, Sordariomycetes,
Ascomycota)
MyriosclerotiniaSclerotiniaceae, Leotiales, Ascomycota
MyrotheciumMitosporic fungi
MystrosporiumMitosporic fungi
NaeviaDermateaceae, Leotiales, Ascomycota (Dermateaceae,
NakataeaMitosporic fungi
NattrassiaMitosporic fungi
NecatorAnamorphic Corticiaceae
NectriaHypocreaceae, Hypocreales, Ascomycota (Nectriaceae,
NectriellaHypocreaceae, Hypocreales, Ascomycota (Bionectriaceae,
NematosporaMetschnikowiaceae, Saccharomycetales, Ascomycota
(Eremotheciaceae, Saccharomycetales, Saccharomycetes, Saccharomycotina, Ascomycota)
NeocosmosporaHypocreaceae, Hypocreales, Ascomycota (Nectriaceae,
NeofabraeDermateaceae, Leotiales, Ascomycota (Dermateaceae,
NeovossiaTilletiaceae, Ustilaginales, Ustomycetes, Basidiomycota
(Tilletiaceae, Tilletiales, Ustilaginomycetes, Basidiomycota)
NigrosporaAnamorphic Trichosphaeriales
NummulariaXylariaceae, Xylariales, Ascomycota (Xylariaceae,
OidiopsisAnamorphic Erysiphaceae
OidiumAnamorphic Erysiphaceae
OlpidiumOlpidiaceae, Spizellomycetales, Chytridiomycetes,
Chytridiomycota (Olpidiacae, Spizellomycetales, Chytridiomycota)
OmnidemptusMagnaporthaceae, Incertae Sedis, Ascomycota
OmphalinaTricholomataceae, Agaricales, Basidiomycetes,
Basidiomycota)
OncobasidiumCeratobasidiaceae, Ceratobasidiales, Basidiomycota

(Ceratobasidiaceae, Tulasnellales, Hymenomycetes, Basidiomycota)
OosporaMitosporic fungi
OperculellaMitosporic fungi
OphiobolusLeptosphaeriaceae, Dothideales, Ascomycota
(Leptosphaeriaceae, Pleosporales, Dothideomycetes, Ascomycota)
OphiosphaerellaPhaeosphaeriaceae, Dothideales, Ascomycota
(Phaeosphaeriaceae, Dothideomycetes et Chaetothyriomycetes Uncertain, Ascomycota)
OphiostomaOphiostomataceae, Ophiostomatales, Ascomycota
(Ophiostomataceae, Ophiostomatales, Sordariomycetidae,
OvulariopsisAnamorphic Erysiphaceae
OxyporusCoriolaceae, Poriales, Basidiomycetes, Basidiomycota
(Rigidiporaceae, Hymenochaetales s.l., Hymenomycetes,
Basidiomycota)
OzoniumMitosporic fungi
PaecilomycesAnamorphic Trichocomaceae
Paracercospora- Anamorphic Mycosphaerellaceae
ParaphaeosphaeriaPhaeosphaeriaceae, Dothideales, Ascomycota
PatellariaPatellariaceae, Patellariales, Ascomycota (Patellariaceae,
Patellariales, Dothideomycetes, Ascomycota)
PatellinaMitosporic fungi
PelliculariaCorticiaceae, Stereales, Basidiomycetes, Basidiomycota
(Corticiaceae, Basidiomycota Families Uncertain, Basidiomycota)
PeltasterMitosporic fungi
PenicilliumAnamorphic Trichocomaceae
PeniophoraPeniophoraceae, Stereales, Basidiomycetes, Basidiomycota
(Peniophoraceae, Basidiomycota Families Uncertain, Basidiomycota)
PerenniporiaCoriolaceae, Poriales, Basidiomycetes, Basidiomycota
(Perenniporiaceae, Polyporales, Hymenomycetes, Basidiomycota)
PericoniaMitosporic fungi
PericoniellaMitosporic fungi
PeronosclerosporaSclerosporaceae, Sclerosporales, Oomycota,
Chromista (Sclerosporaceae, Sclerosporales, Oomycetes, Chromista)
PeronosporaPeronosporaceae, Peronosporales, Oomycota, Chromista
PestalotiaMitosporic fungi
PestalotiopsisAnamorphic Amphisphaeriaceae
PestalozziaMitosporic fungi
PeziculaDermateaceae, Leotiales, Ascomycota (Dermateaceae,

PezizellaLeotiaceae, Leotiales, Ascomycota (Thelebolaceae,
PhacidiopycnisAnamorphic Cryptomycetaceae
PhacidiumPhacidaceae, Leotiales, Ascomycota (Phacidaceae,
PhaeoacremoniumMitosporic fungi
PhaeocytosporellaMitosporic fungi
PhaeocytostromaMitosporic fungi
PhaeoisariopsisMitosporic fungi
PhaeolusCoriolaceae, Poriales, Basidiomycetes, Basidiomycota
PhaeoramulariaMitosporic fungi
PhaeoseptoriaMitosporic fungi
PhaeosphaerellaVenturiaceae, Dothideales, Ascomycota (Venturiaceae,
PhaeosphaeriaPhaeosphaeriaceae, Dothideales, Ascomycota
PhaeosphaerulinaDothioraceae, Dothideales, Ascomycota
(Dothioraceae, Dothideomycetes et Chaetothyriomycetes Uncertain,
Ascomycota)
PhakopsoraPhakopsoraceae, Uredinales, Teliomycetes, Basidiomycota
PhellinusHymenochaetaceae, Hymenochaetales, Basidiomycetes,
Basidiomycota (Hymenochaetaceae, Hymenochaetales s.str.,
PhialophoraAnamorphic Magnaporthaceae
PhomaAnamorphic Pleosporaceae
PhomopsisAnamorphic Valsaceae
PhragmidiumPhragmidiaceae, Uredinales, Teliomycetes,
Basidiomycota (Phragmidiaceae, Pucciniales, Teliomycetes,
Basidiomycota)
PhyllachoraPhyllachoraceae, Phyllachorales, Ascomycota
Ascomycota)
PhyllactiniaErysiphaceae, Erysiphales, Ascomycota (Erysiphaceae,
PhyllostictaAnamorphic Mycosphaerellaceae
PhyllostictellaMitosporic fungi
PhymatotrichopsisAnamorphic Sistrotremataceae
PhymatotrichumMitosporic fungi
PhysalosporaHyponectriaceae, Familia Incertae Sedis, Ascomycota
PhysarumPhysaraceae, Physarales, Myxomycota (Physaraceae,
Physarales, Myxomycetes, Myxomycota)
PhysodermaPhysodermataceae, Blastocladiales, Chytridiomycota
(Physodermataceae, Blastocladiales, Chytridiomycota)
PhysopellaUredinales Incertae Sedis, Basidiomycota (Basidiomycota
Genera Uncertain, Basidiomycota Families Uncertain, Basidiomycota)
PhytophthoraPythiaceae, Pythiales, Oomycota, Chromista (Pythiaceae,
Pythiales, Oomycetes, Chromista)
PichiaSaccharomycetaceae, Saccharomycetales, Ascomycota
Ascomycota)
PilidiellaMitosporic fungi
PithomycesAnamorphic Pleosporaceae
PlasmodiophoraPlasmodiophoraceae, Plasmodiophorales,
Plasmodiophoromycota, Protozoa (Plasmodiophoraceae,
Plasmodiophorales, Plasmodiophoromycota, Protozoa)
PlasmoparaPeronosporaceae, Peronosporales, Oomycota, Chromista
PlatysporaHysteriaceae, Dothideales, Ascomycota (Diademaceae,
PlenodomasMitosporic fungi
PleocytaMitosporic fungi
PleosphaerulinaDothioraceae, Dothideales, Ascomycota
(Dothioraceae, Dothideomycetes et Chaetothyriomycetes Uncertain,
Ascomycota)
PleosporaPleosporaceae, Dothideales, Ascomycota (Pleosporaceae,
PodosphaeraErysiphaceae, Erysiphales, Ascomycota (Erysiphaceae,
PolyporusPolyporaceae, Poriales, Basidiomycetes, Basidiomycota
(Polyporaceae, Polyporales, Hymenomycetes, Basidiomycota)
PolyscytalumMitosporic fungi
PolystigmaPhyllachoraceae, Phyllachorales, Ascomycota
Ascomycota)
PotebniamycesCryptomycetaceae, Rhytismatales, Ascomycota
(Cryptomycetaceae, Rhytismatales, Leotiomycetes, Ascomycota)
ProventuriaVenturiaceae, Dothideales, Ascomycota (Venturiaceae,
PseudocercosporaMitosporic fungi
PseudocercosporellaAnamorphic Mycospherellaceae
PseudocochliobolusPleosporaceae, Dothideales, Ascomycota
(Pleosporaceae, Pleosporales, Dothideomycetes, Ascomycota)
PseudoepicoccumMitosporic fungi
PseudoperonosporaPeronosporaceae, Peronosporales, Oomycota,
Chromista (Peronosporaceae, Peronosporales, Oomycetes, Chromista)
PseudopeziculaLeotiaceae, Leotiales, Ascomycota (Leotiaceae,
PseudopezizaDermateaceae, Leotiales, Ascomycota (Dermateaceae,
PseudophaeolusCoriolaceae, Poriales, Basidiomycetes, Basidiomycota
PseudoseptoriaMitosporic fungi
PsilocybeStrophariaceae, Agaricales, Basidiomycetes, Basidiomycota
(Strophariaceae, Agaricales, Hymenomycetes, Basidiomycota)
PucciniaPucciniaceae, Uredinales, Teliomycetes, Basidiomycota
(Pucciniaceae, Pucciniales, Teliomycetes, Basidiomycota)
Pucciniastrum- Pucciniastraceae, Uredinales, Teliomycetes,
Basidiomycota (Pucciniastraceae, Pucciniales, Teliomycetes,
Basidiomycota)
PycnoporusCoriolaceae, Poriales, Basidiomycetes, Basidiomycota
PyrenobotrysVenturiaceae, Dothideales, Ascomycota (Venturiaceae,
PyrenochaetaAnamorphic Lophiostomataceae
PyrenopezizaDermateaceae, Leotiales, Ascomycota (Dermateaceae,
PyrenophoraPleosporaceae, Dothideales, Ascomycota (Pleosporaceae,
PyriculariaAnamorphic Magnaporthaceae
PythiumPythiaceae, Pythiales, Oomycota, Chromista (Pythiaceae,
Pythiales, Oomycetes, Chromista)
RamichloridiumMitosporic fungi
RamulariaAnamorphic Dothideaceae
RamulisporaMitosporic fungi
RhinocladiumMitosporic fungi
RhizoctoniaAnamorphic Corticiaceae, Ceratobasidiaceae, Otideaceae
RhizomorphaFungi, Incertae Sedis
RhizopusMucoraceae, Mucorales, Zygomycetes, Zygomycota
RhizosphaeraAnamorphic Venturiaceae
RhopographusPhaeosphaeriaceae, Dothideales, Ascomycota

RhynchosporiumMitosporic fungi
RhytismaRhytismataceae, Rhytismatales, Ascomycota
(Rhytismataceae, Rhytismatales, Leotiomycetes, Ascomycota)
RigidoporusCoriolaceae, Poriales, Basidiomycetes, Basidiomycota
(Rigidiporaceae, Hymenochaetales s.l., Hymenomycetes,
Basidiomycota)
RoesleriaCaliciales Incertae Sedis, Ascomycota (Ascomycota Genera
Uncertain)
RoselliniaXylariaceae, Xylariales, Ascomycota (Xylariaceae,
SaccharomycesSaccharomycetaceae, Saccharomycetales, Ascomycota
Saccharomycotina, Ascomycota)
SalmoniaErysiphaceae, Erysiphales, Ascomycota (Erysiphaceae,
SarocladiumMitosporic fungi
SchizoparmeMelanconidaceae, Diaporthales, Ascomycota
(Melanconidaceae, Diaporthales, Sordariomycetidae, Sordariomycetes,
Ascomycota)
SchizophyllumSchizophyllaceae, Schizophyllales, Basidiomycetes,
Basidiomycota (Schizophyllaceae, Agaricales, Hymenomycetes,
Basidiomycota)
SchizothyriumSchizothyriaceae, Dothideales, Ascomycota
(Schizothyriaceae, Dothideomycetes et Chaetothyriomycetes Uncertain, Ascomycota)
SclerophthoraVerrucalvaceae, Sclerosporales, Oomycota, Chromista
(Verrucalvaceae, Sclerosporales, Oomycetes, Chromista)
SclerosporaSclerosporaceae, Sclerosporales, Oomycota, Chromista
(Sclerosporaceae, Sclerosporales, Oomycetes, Chromista)
SclerotiniaSclerotiniaceae, Leotiales, Ascomycota (Sclerotiniaceae,
SclerotiumMitosporic fungi
ScolecosporiellaMitosporic fungi
ScolicotrichumMitosporic fungi
ScopulariopsisAnamorphic Microascaceae
ScytalidiumMitosporic fungi
ScytinostromaLachnocladiaceae, Lachnocladiales, Basidiomycetes,
Basidiomycota (Lachnocladiaceae, Lachnocladiales, Hymenomycetes,
Basidiomycota)
SeimatosporiumAnamorphic Amphisphaeriaceae
SelenophomaMitosporic fungi
SeptobasidiumSeptobasidiaceae, Septobasidiales, Teliomycetes,
Basidiomycota (Septobasidiaceae, Septobasidiales s.str., Teliomycetes,
Basidiomycota)
SeptocylindriumMitosporic fungi
SeptogloeumMitosporic fungi
SeptoriaAnamorphic Dothideaceae
SerpulaConiophoraceae, Boletales, Basidiomycota (Boletaceae,
Boletales, Hymenomycetes, Basidiomycota)
SetosphaeriaPleosporaceae, Dothideales, Ascomycota (Pleosporaceae,
SitosporiumMitosporic fungi
SoleniaSchizophyllaceae, Schizopyllales, Basidiomycota
(Schizophyllaceae, Agaricales, Hymenomycetes, Basidiomycota)
SphaceliaAnamorphic Clavicipitaceae
SphacelomaAnamorphic Elsinoaceae
SphacelothecaUstilaginaceae, Ustilaginales, Ustomycetes,
Basidiomycota (Ustilaginaceae, Ustilaginales, Ustilaginomycetes.
Basidiomycota)
SphaerodothisPhyllachoraceae, Phyllachorales, Ascomycota
Ascomycota)
SphaeropsisMitosporic fungi
SphaerostilbeHypocreaceae, Hypocreales, Ascomycota (Nectriaceae,
SphaerothecaErysiphaceae, Erysiphales, Ascomycota (Erysiphaceae,
SphaerulinaMycospherellaceae, Dothideales, Ascomycota
SpilocaeaAnamorphic Venturiaceae
SpondylocladiumMitosporic fungi
SpongosporaPlasmodiophoraceae, Plasmodiophorales,
Plasmodiophoromycota, Protozoa (Plasmodiophoraceae,
Plasmodiophorales, Plasmodiophoromycota, Protozoa)
SporendonemaMitosporic fungi
SporisoriumUstilaginaceae, Ustilaginales, Ustomycetes, Basidiomycota
(Ustilaginaceae, Ustilaginales, Ustilaginomycetes, Basidiomycota)
SporobolomycesMitosporic fungi
SporonemaMitosporic fungi
SporotrichumMitosporic fungi
StagonosporaMitosporic fungi
StagonosporopsisMitosporic fungi
SteccherinumSteccherinaceae, Stereales, Basidiomycota
Basidiomycota)
StemphyliumAnamorphic Pleosporaceae
StenocarpellaMitosporic fungi
StereumStereaceae, Stereales, Basidiomycota (Stereaceae, Hericiales,
StigmateaAscomycota, Incertae Sedis (Ascomycota Genera Uncertain)
StigminaAnamorphic Dothideales
StilbumChionosphaeraceae, Atractiellales, Basidiomycetes,
Basidiomycota (Chionosphaeraceae, Basidiomycota Families Uncertain, Basidiomycota)
SynchronoblastiaMitosporic fungi
SynchytriumSynchytriaceae, Chytridiales, Chytridiomycota
(Synchytriaceae, Synchytridiales, Chytridiomycota)
TapesiaDermateaceae, Leotiales, Ascomycota (Dermateaceae,
TaphrinaTaphrinaceae, Taphrinales, Ascomycota (Taphrinaceae,
Taphrinales, Taphrinomycetes, Taphrinomycotina, Ascomycota)
ThanatephorusCeratobasidiaceae, Ceratobasidiales, Basidiomycetes,
Basidiomycota (Ceratobasidiaceae, Tulasnellales, Hymenomycetes,
Basidiomycota)
ThielaviopsisMitosporic fungi
ThryosporaMitosporic fungi
ThyrostromaMitosporic fungi
TilletiaTilletiaceae, Ustilaginales, Ustomycetes, Basidiomycota
(Tilletiaceae, Tilletiales, Ustilaginomycetes, Basidiomycota)
TolyposporiumUstilaginaceae, Ustilaginales, Ustomycetes,
Basidiomycota (Ustilaginaceae, Ustilaginales, Ustilaginomycetes.
Basidiomycota)
TrachysphaeraPythiaceae, Pythiales, Oomycota, Chromista
(Pythiaceae, Pythiales, Oomycetes, Chromista)
TrametesCoriolaceae, Poriales, Basidiomycetes, Basidiomycota
TranzscheliaUropyxidaceae, Uredinales, Teliomycetes, Basidiomycota
(Uropyxidaceae, Pucciniales, Teliomycetes, Basidiomycota)
TrechisporaSistrotremataceae, Stereales, Basidiomycetes,
Basidiomycota (Sistrotremataceae, Basidiomycota Families Uncertain,
Basidiomycota)
TrichaptumCoriolaceae, Poriales, Basidiomycetes, Basidiomycota

Basidiomycota)
TrichoconisMitosporic fungi
TrichoconiumMitosporic fungi
TrichodermaAnamorphic Hypocreaceae
TrichometasphaeriaLophiostomataceae, Dothideales, Ascomycota
(Lophiostomataceae, Pleosporales, Dothideomycetes, Ascomycota)
TrichotheciumMitosporic fungi
TripospermumAnamorphic Capnodiaceae
TuberculariaAnamorphic Hypocreaceae
TunstalliaAscomycota Incertae Sedis
TyphulaTyphulaceae, Cantharellales, Basidiomycetes, Basidiomycota
(Basidiomycota Genera Uncertain, Basidiomycota Families Uncertain,
Basidiomycota)
UlocladiumMitosporic fungi
UncinulaErysiphaceae, Erysiphales, Ascomycota (Erysiphaceae,
UredoAnamorphic Uredinales (Basidiomycota Genera Uncertain,
Basidiomycota Families Uncertain, Basidiomycota)
UrocystisTilletiaceae, Ustilaginales, Ustomycetes, Basidiomycota
(Urocystaceae, Urocystales, Ustilaginomycetes, Basidiomycota)
UromycesPucciniaceae, Uredinales, Teliomycetes, Basidiomycota
(Pucciniaceae, Pucciniales, Teliomycetes, Basidiomycota)
UrophlyctisUrophlyctidiaceae, Spizellomycetales, Chytridiomycetes,
Chytridiomycota (Urophlyctidiaceae, Spizellomycetales,
Chytridiomycota)
UstilagoUstilaginaceae, Ustilaginales, Ustomycetes, Basidiomycota
(Ustilaginaceae, Ustilaginales, Ustilaginomycetes. Basidiomycota)
UstulilaginoideaMitosporic fungi
UstulinaXylariaceae, Xylariales, Ascomycota (Xylariaceae, Xylariales,
Xylariomycetidae, Sordariomycetes, Ascomycota)
ValsaValsaceae, Diaporthales, Ascomycota (Valsaceae, Diaporthales,
Sordariomycetidae, Sordariomycetes, Ascomycota)
VenturiaVenturiaceae, Dothideales, Ascomycota (Venturiaceae,
VeronaeaMitosporic fungi
VerticilliumMitosporic fungi
WaiteaBotryobasidiaceae, Stereales, Basidiomycetes, Basidiomycota
(Ceratobasidiaceae, Tulasnellales, Hymenomycetes, Basidiomycota)
Whetzelinia Sclerotiniaceae, Leotiales, Ascomycota (Sclerotiniaceae,
WilsonomycesMitosporic fungi
XylariaXylariaceae, Xylariales, Ascomycota (Xylariaceae, Xylariales,
Xylariomycetidae, Sordariomycetes, Ascomycota)
ZimmermaniellaPhyllachoraceae, Phyllachorales, Ascomycota
Ascomycota)
ZopfiaZopfiaceae, Dothideales, Ascomycota (Zopfiaceae,
ZygophialaMitosporic fungi
ZygosaccharomycesSaccharomycetaceae, Saccharomycetales,
Ascomycota (Saccharomycetaceae, Saccharomycetales,
Saccharomycetes, Saccharomycotina, Ascomycota)
ZythiaMitosporic fungi
REFERENCES
Barr, D. J. S. (1992). Evolution and kingdoms of organisms from the perspective of
a mycologist. Mycologia, 84:1-11.
FungalWeb (n.d.). <http://www.fungalweb.com>.
Hawksworth, D. L., Kirk, P. M., Sutton, B. C., and Pegler, D. N. (1995). Ainsworth
and Bisbys Dictionary of the Fungi. CAB International, U.K.
Vidhyasekaran, P. (1993). Principles of Plant Pathology. CBS Publishers, Delhi,
India.
Wrobel, W. and Creber, G. (1998). Elseviers Dictionary of Fungi and Fungal Plant
Diseases. Elsevier, Amsterdam, Holland.
Parasitic
Parasitic
Flagellate
Flagellate Protozoa
Protozoa
In addition to pathogens, plant diseases are also caused by parasites.
Pathogens cause harmful deviation from normal functioning of physiological processes, whereas parasites get their food from the plant. A parasite is
defined as an organism that lives in or on the cells of another organism and
obtains its food from the latter without necessarily causing disease symptoms (Strobel and Barash, 1990). However, they can cause disease symptoms,
probably by depleting host nutrients. The flagellated protozoa of the genus
Phytomonas and some Herpetomonas spp. are parasites of plants. The parasitism of protozoa occurs without any apparent pathogenicity (Dutra et al.,
2000). The parasites live mostly in the phloem and laticifers of infected
plants. Such parasites can also cause diseases of economic significance in
plantations of coconut, oil palm, cassava, and coffee (Dollet, 1984; Camargo,
1990). Parasites have been detected in edible fruits such as pomegranates,
peaches, guavas, and tangerines (Dutra et al., 2000). Phytomonas is the important genus causing diseases. The important diseases caused by Phytomonas are described here.
PHLOEM NECROSIS OF COFFEE
Phytomonas parasitizes coffee plants and causes phloem necrosis. The
parasitized coffee plants show a reduction in their starch reserves. The oldest leaves turn yellow and prematurely fall. The new leaves are fewer and
smaller than on a healthy tree. They get paler until they yellow and then fall,
leaving bare branches. The diseased tree dies in three to 12 months. In the
acute form of the disease, only a few old leaves fall; the others lose
turgescence and hang limp without falling (Dollet, 1984). After two to three
weeks, the leaves turn brownish and necrotic. The roots also turn brown and
perish. Cytological examinations show hyperplasia of the phloem, producing sieve tubes three times smaller than normal. Browning of cells and deposition of callose are seen. The vector of the disease is not known. Scale insects may be involved in the transmission of the disease.
HARTROT OF COCONUT
Hartrot of coconut is otherwise called lethal yellowing, bronze wilt, or
Coronie wilt. The earliest symptom is yellowing of the oldest leaves, followed by yellowing of the younger leaves. The unripe coconuts may fall.
These nuts show internal browning of the husk and blackening of the
endocarp. The petioles of the oldest leaves break and necrosis starts in the
spear. The apical region of the crown rots, producing a foul odor. Two Pentatomidae insects, Lincus croupius and L. styliger, are believed to transmit
the disease (Desmier de Chenon et al., 1983).
MARCHITEZ DISEASE OF OIL PALM
Early symptoms of Marchitez disease include browning of the leaflet
tips. The root system deteriorates, growth slows down, and fruit bunches become dull and rot or fall. A Pentatomidae insect of genus Lincus may be the
vector of the disease. Insecticides reduce the spread of the disease.
Not much work has been done on Phytomonas, most likely because it
does not affect major crops and does not cause any significant economic
losses.
REFERENCES
Camargo, E. P. (1990). Phytomonas and other trypanosomatid parasites of plants
and fruit. Adv Parasitol, 42:29-112.
Desmier de Chenon, R., Merlan, E., Genty, P., Morin, J. P., and Doller, M. (1983).
Research on the genus Lincus, Pentatomidae Discocephalidae and its possible
role in the transmission of the Marchitez of oil palm and Hart rot of coconut.
Presented in 4a Reun Com Tecn Reg San Veg SARAHIICA, Cancun, Mexico.
Dollet, M. (1984). Plant diseases caused by Flagellate protozoa (Phytomonas).
Annu Rev Phytopathol, 22:115-132.
Dutra, P. M. L., Rodrigues, C. O., Romeiro, A., Grillo, L. A. M., Dias, F. A., Attias,
M., De Souza, W., Lopes, A. H. C. S., and Meyer-Fernandes, J. R. (2000). Characterization of ectophosphatase activities in trypanosomatid parasites of plants.
Phytopathology, 90:1032-1038.
Strobel, G. A. and Barash, I. (1990). Microbial phytotoxins and plant diseases. In
P. Vidhyasekaran (Ed.), Basic Research for Crop Disease Management, Daya
Publishing House, Delhi, pp. 65-73.
Parasitic
ParasiticGreen
Green Algae
Algae
Algae that are parasitic on plants are known only among the phylum
Chlorophyta. Of those, only the genus Cephaleuros (family Trentepohliaceae order Trentepohliales) is known to cause diseases in economically
important crops. The diseases caused by Cephaleuros spp. are commonly
called red rusts or algal spots.
ECONOMIC IMPORTANCE
Cephaleuros virescens (= C. mycoidea) is known to cause diseases in
Citrus spp. (e.g., limes, lemons, oranges, grapefruit), mango, papaya, pecan, avocado, cacao, tea, coffee, pepper, oil palms, vanilla, litchi, sapota,
and guava. Cephaleuros virescens is prevalent in India, Indonesia, China,
Japan, Malaysia, Australia, the United States, Brazil, West Indies, and Africa. In general, the alga affects perennial trees, not annual crops. It does not
cause economical losses in many crops. However, considerable losses in
tea, pepper, and Citrus spp. due to the alga have been reported.
DISEASE SYMPTOMS
Cephaleuros virescens infects the leaves, stems, and fruit of the trees.
Leaf infection can be seen on the lamina, veins, and petiole. Yellow-green
pinpoint specks appear on the upper leaf surface. Occasionally the specks
occur on the lower leaf surface. The algal thallus grows mostly subcuticular
and the cuticle imparts a glistening appearance to the early developmental
stage. As growth of the disc progresses, sporophores and sterile hairs develop, both of which contain the orange pigment hematochrome. The algal
colony becomes velvety in texture and its color changes from green to orange brown. The thallus often becomes slightly raised. Green islands
around the infection foci are common. The cell layers nearest to the alga die
and assume a corklike appearance. They may serve as a barrier to further al-
gal penetration. Only a vigorous host is capable of producing an effective

barrier. The alga takes up water and minerals from the host by osmosis.
In tea leaves, an intercellular type of Cephaleuros infection is often seen.
A small purple translucent spot gradually extends from the upper to the
lower surface. On such lesions, fructifications of the alga can be seen on
both surfaces. Ultimately, the affected region dries and drops out leaving
holes in the affected leaves (shot hole pattern). In coffee leaves, the spongy
mesophyll cells enlarge, divide, and undergo considerable cell wall thickening. The alga appears to be completely isolated from the healthy tissue by a
continuous barrier of thick-walled cells (Joubert and Rijkenberg, 1971).
Stem infection by the algal parasite results in the appearance of dark gray
or purple lesions. These lesions assume the typical rusty red appearance
similar to those found in leaf infections. The bark becomes scaly and cracks.
Localized swelling is common at the site of infection. Lesions coalesce, often girdling the stem completely for a long distance. Young wood may die
due to infection. Affected branches are often stunted and bear fewer leaves,
which typically show signs of chlorosis and variegation. Fruit infections are
common on guava, citrus, tea, and avocado and coffee berries. Slightly
raised, irregularly shaped, dark green, brown, or black lesions appear on
fruits. The alga penetrates up to the superficial cell layers of the fruit only.
LIFE CYCLE
Cephaleuros spp. have a disclike thallus composed of symmetrically arranged cells that radiate dichotomously from the center to the periphery
(Joubert and Rijkenberg, 1971). The cells are elongated and sometimes barrel shaped. The thallus cells at the margin are monolayered, whereas several
layers develop in the center of older discs. The algal filaments usually extend between the cuticle and the epidermis of host leaves or, more rarely, between the epidermis and palisade cells. Sometimes, the parasite penetrates
between the mesophyll cells of the leaf. The alga appears to extend by the
mechanical force of its expansion rather than by enzymatic dissolution of
host structures. A large airspace is found between the algal disc and the necrotic epidermal cells of the host. Rhizoids arise from the thallus and extend
through the airspace to the underlying host cells. The rhizoids serve to anchor the thallus and furnish it with water and other substances from the host
(Vidhyasekaran, 1993).
Large, sessile, flask-shaped cells known as gametangia arise in the thallus.
In the presence of free water, these cells open by fissure and release swarm
spores. Eight to 32, but most commonly 16, biflagellate isogametes per
gametangium are found. Copulation takes place between two gametes
either from the same gametangium or from different gamentangia. The resulting zygote produces a dwarf sporophyte that bears a small, dehiscent
microsporangium. After meiotic division, four quardiflagellate microzoospores are produced (Joubert and Rijkenberg, 1971). These microzoospores
rarely infect the host. The older algal discs produce hairlike initials at the
end of some of the radiating rows of cells. After rupturing the cuticle or epidermis of the host, the hairlike initials differentiate into either setae or
sporangiophores, both of which are produced abundantly on the same thallus.
The sporangiophores terminate in a swollen apical cell from which many
lateral protrusions are subtended. Each protrusion develops into a zoosporangium attached to the apex by a pedicel. Under favorable conditions,
sporangia release zoospores through an ostiole. Zoospores are biflagellate,
and about 30 are produced from each sporangium. When the zoospore
reaches the host cell, it comes to rest. A supracuticular primary disc develops as a result of repeated cross-wall formation in the zoospore after it
has come to rest. Buds develop from the undersurface of the primary disc
cells, penetrate the host leaf, and form a secondary disc in the plant tissue.
There are also reports that nearly all superficial thalli perish and that only
those zoospores that lodge in a crevice or abrasion succeed in infecting the
host (Wolf, 1930). The infection may be initiated by zoospores washed into
the stomata by rain. Zoosporangia are disseminated by the wind. Rain water
running over the surfaces of lesions and splashing onto new leaves may rapidly spread the zoospores. Hence, at the end of rainy season the disease becomes severe (Mann and Hutchinson, 1907).
DISEASE MANAGEMENT
Host debility is usually accompanied by an increased incidence and severity of the disease, which can be significantly reduced by the application
of nitrogen and potassium. Disease incidence also can be reduced by providing proper soil drainage. All badly diseased or dead wood should be removed. Pruning is recommended for citrus, tea, and cacao trees. Shade trees
and shelterbelts may serve as the primary sources of inoculum. Hence,
shade trees selected should not be alternate hosts for the algal pathogen. Irrigation may reduce the disease incidence by improving the vigor of the trees.
Fungicide sprays may reduce the algal infection. Bordeaux mixture is used
extensively in the control of algae. Copper oxychloride and cuprous oxide
can be sprayed to control the disease.
REFERENCES
Joubert, J. J. and Rijkenberg, F. H. J. (1971). Parasitic green algae. Annu Rev
Phytopathol, 9:45-64.
Mann, H. H. and Hutchinson, C. M. (1907). Cephaleuros virescens Kunze the red
rust of tea. Mem Dep Agr India Bot Ser, 1(6):1-33.
Vidhyasekaran, P. (1993). Principles of Plant Pathology. CBS Publishers, Delhi.
Wolf, F. A. (1930). A parasitic alga, Cephaleuros virescens Kunze, on Citrus and
certain other plants. J Elisha Mitchell Sci Soc, 45:187-205.
Parasitic
ParasiticHigher
Higher Plants
Plants
Some plants are parasitic to other plants, depending upon them for food
and/or water. Such plants cause disease by taking away the nutrients of the
host. The important parasitic higher plants are Striga, Orobanche, Loranthus,
Cassytha, and Cuscuta.
STRIGA
Several Striga species parasitize crop plants. More than 30 species of
Striga have been reported in Africa (Berner et al., 1995). Three of these species are endemic to Australia. Two species, Striga asiatica and S. gesnerioides, have been detected in the United States. Striga spp., which are also
called witchweed, affect many crops, including corn, sorghum, sugarcane,
rice, cowpea, and tobacco. Striga are characterized by opposite leaves and
irregular flowers with a pronounced bend in the corolla tube. They are obligate parasites and will not develop without hosts. They are also root parasites that produce haustoria, food-absorbing outgrowths that graft with the
roots of the host. Striga spp. lack typical root hairs and root caps (Vidhyasekaran, 1993).
The seeds of Striga spp. are extremely small and require afterripening or
postharvest ripening before germination. Germination of seeds requires exposure to an exogenous germination stimulant after an environmental conditioning period in which the seeds imbibe water. Usually this stimulant is a
host-root exudate, but some nonhost-root exudates and synthetic compounds can also stimulate germination of Striga seeds. The stimulant in the
root exudate has been identified as strigol. Strigol promotes the germination
of S. asiatica seeds at concentrations as low as 1016 M. Ethylene, gibberellins, cytokinins, and coumarins also stimulate the germination of Striga
seeds. After germination, endosperm nutrients can sustain the seedlings for
three to seven days in the absence of a host. If the seedlings do not attach to a
host and successfully establish a parasitic link within this period, they will
die. If a host root is in close proximity (2 to 3 mm) to a germinated Striga
seedling, chemical signals are exchanged that direct the seedlings radicle to
the host root, initiate haustorium induction, and result in the successful attachment and establishment of xylem-to-xylem connections between the
parasite and host (Berner et al., 1995). One of these signals has been identified as 2,6-dimethoxy-p-benzoquinone, which may be the product of enzymatic degradation of the host root responsible for stimulating formation of
the parasite haustorium. Haustoria of parasitic plants are specialized organs
developed from parasite radicles prior to penetration. After successful attachment, developing Striga plants grow underground for four to seven
weeks prior to emergence. Numerous parasitic attachments occur on the
same plant.
Striga absorb water and foodstuffs through their haustoria. Carbohydrate
and nitrogen of the host are utilized by the parasites. This process results in
sugar sink and nitrogen starvation in the host. Differential absorption of
phosphorous, potassium, sulfur, and iron by the infected host has also been
reported. Infected sorghum plants show 90 to 95 percent less cytokinins and
30 to 80 percent less gibberellins. A strong transpirational pull from host to
parasite is observed. High humidity inhibits the growth of Striga due to a reduced flow of materials from host to parasite. For the same reason, Striga
survive more often in dry soils. Striga strengthen the roots that are parasitized
and stimulate root production of the host (Vidhyasekaran, 1993).
Symptoms of parasitism resemble drought stress, nutrient deficiency,
and vascular disease. Infected plants become stunted with heavy yield production. Parasites blossom and form fruits within three or four weeks of
emergence, producing several hundred thousand minute seeds per plant.
The seeds are dispersed by the wind, finally settling on the soil. Such seeds
may be viable for up to 14 years in soil.
Biological strains of Striga have been reported. S. gesnerioides isolates
from tobacco do not infect cowpea and the isolates from cowpea do not infect tobacco. The Sorghum strain of S. hermonthica does not infect corn and
the corn strain of S. hermonthica does not infect sorghum. Cowpea cultivarspecific races of S. gesnerioides have been reported also. Striga seeds are
often found mixed with crop seeds, and the contaminated crop seeds may
transmit the disease into a new area of the field. Hence, seeds should be obtained only from Striga-free fields. Seed treatment by soaking seeds in an
aqueous solution with low amounts of imazaquin (an acetolactate synthase
[ALS] inhibitor that selectively interferes with inhibition of amino acid
biosynthesis by the parasite) effectively contols Striga infection in cowpea
(Berner et al., 1994). Crop varieties resistant to the parasites have been identified. Four cowpea cultivars, Gorom Local (SUVITA-2) from Burkina
Faso, 58-57 from Senegal, B301 from Botswana, and IT82D-849 from Nigeria, have been identified as resistant to S. gesnerioides. However, several
races of Striga are known in different countries and some of these resistant
cowpea cultivars are susceptible to new races of S. gesnerioides (Berner

et al., 1995).
Some cultural practices may reduce parasite incidence. Soil mulching
with polyethylene film can be useful in controlling Striga seed germination.
Soil fumigation with methyl bromide kills Striga seeds. Soil injection of
ethylene gas is used in the United States to induce Striga seed germination
in the absence of a host crop. Trap crops, which stimulate germination but
do not support parasitism, can be grown to remove Striga from the infested
field. Cotton and peanut are trap crops for S. asiatica, and sudangrass is a
trap crop for S. hermonthica. Parasites should be pulled out and destroyed
before their seeds set. Weedicides such as 2,4-D and MCPA can be useful in
controlling Striga.
OROBANCHE
Orobanche is commonly called broomrape. Several species of Orobanche
attack crops, and at least seven species attack tobacco. Orobanche aegyptica
and O. cernua affect tobacco, sunflowers, melons, tomatoes, and broadbeans. Tobacco is seriously affected by Orobanche in India, Pakistan, Russia, Mexico, Italy, Australia, New Zealand, and many Eastern European
countries. Orobanche is a minor parasite in the United States. It is an annual
fleshy flower plant, growing up to a height of about 12 inches. It has a cylindrical, thick, fleshy, whitish to purple stem. The stem bears scaly leaves that
end in spikes. It bears many tiny flowers that vary from purple to white in
color.
It is an obligate parasite that carries no chlorophyll. The single stem is attached to a tobacco root, ten or more of which can be carried by one tobacco
plant. Orobanche produces abundant minute seeds. The seeds remain dormant in the soil for more than 20 years. Germination occurs only when the
seeds are close to the roots of plants that are able to provide necessary stimulation. Stimulants in the root exudates have been identified as benzopyran
derivatives. Several growth regulators, such as ethylene, gibberellins, cytokinins, and coumarins, also induce seed germination. Orobanche emerges
five to six weeks after tobacco is planted and as many as 50 shoots may be
found around a single plant. Affected plants are stunted and chlorosis of the
leaves is predominant. Orobanche shoots develop singly or in bunches, and
they are attached to host roots in a fragile manner by swollen cloves. They
grow quickly, sometimes rising above the height of the host plant. Some cultural practices, such as hand weeding, may reduce parasite incidence.
Carum ajown, a trap crop for O. cernua, can be exploited for the control of
Orobanche. Methyl bromide fumigation and metham-sodium applied as a
preplanting drench can reduce parasite incidence. Allyl alcohol, dazomet,

and methyl isothiocyanate are also effective in reducing the emergence of
Orobanche in the field.
LORANTHUS
Loranthus is a stem parasite that attacks orange, mango, guava, pomegranate, mulberry, rubber, teak, sandalwood, rosewood, kapok, and casuarina trees. Loranthus has green leaves and small berries that are attractive to
birds. Birds eat the fruit and the seeds are defecated. The defecated seeds adhere to the branches due to their sticking properties. The seed coat is gelatinous and able to absorb water from rain, mist, or dew. This property prevents the seed from perishing. The natural fall of berries from higher to
lower levels of the same host tree ensures a continuous infestation. The
seeds sprout in due course, growing into the host tissue by producing a holdfast. The holdfast may become as large as a tennis ball, completely surrounding the branch. The tops of every infected branch die. Very few leaves
are formed, and losses ranging from 20 to 30 percent of the harvest have
been reported. For effective control, parasitized branches should be cut. To
kill the parasite, one hole is made by drilling at the bottom of the Loranthus
and a mixture of 7 g of copper sulfate and 1 g of 2,4-D is applied. Refined
diesel oil is also used. Forty percent emulsion of the diesel oil is prepared in
water containing 0.005 percent washing soap used as an emulsifying agent,
which is then sprayed on Loranthus.
CASSYTHA
The common species is Cassytha filiformis, which attacks several plants.
It is a slender, threadlike, cylindrical herbaceous vine. Leaves are reduced to
mere scales. Cassytha covers other vegetation like a mantle. It attaches itself
to the host by means of haustoria. It has a spicy fragrance and produces fruit,
globose drupes that are one to two inches in diameter and covered by a
fleshy receptacle. Birds eat these fleshy fruits and seeds and distribute them.
Orange trees are often affected by this parasite. Cassytha should be removed manually before it seeds.
CUSCUTA
Cuscuta is commonly called dodder. Dodder affects many crops, including alfalfa, clover, and tobacco. Dodder is threadlike, with leafless stems
that are yellow, orange, or greenish in color. Minute leaves, which are functionless, are also seen in some species. Dodder vines usually appear as a tangled mass of yellowish threads. Dodder seeds can germinate without external root stimulation. The dodder first produces a rudimentary root system of
its own. However, if the aerial parts are unsuccessful in finding a host plant
within a few weeks, the vine dies. Dodder obtains water and nutrients from
the host plant through haustoria that are embedded in the stems of the host
plant. A close orientation of the vascular elements of the host and the parasite is seen. Some dodders have traces of chlorophyll, and stomata can be
seen on the stem.
Dodder plants produce small white flowers and abundant seeds. The
seeds fall on the soil and germinate, producing tiny colorless seedlings that
evidence a spiral growth at the uppermost portions. These seedlings will
survive only for a short time if a host is not available. When a host is present,
the seedlings will attach to the host. After contact between the host and the
parasite is established, the portion of the seedling embedded in the soil gradually shrivels and disappears. Parasitic plants can be manually removed. Fumigation of the tobacco seedbed with methyl bromide is recommended to
kill dodder seeds in tobacco cultivation.
REFERENCES
Berner, D. K., Awad, A. E., and Algbokhan, E. I. (1994). Potential of imazaquin
seed treatment for control of Striga gesnerioides and Alectra vogelii in cowpea
(Vigna unguiculata). Plant Dis, 78:18-23.
Berner, D. K., Kling, J. G., and B. B. Singh (1995). Striga research and control
A perspective from Africa. Plant Dis, 79:652-660.
Parasitic
Parasitic Nematodes
Nematodes
Nematodes are a large group of invertebrates living in soil and water.
Some feed on higher plants and can cause diseases. Some plant parasitic
nematodes cause heavy crop losses. Cereal cyst nematode Heterodera
avenae, potato cyst nematode Globodora rostochiensis, and the root-knot
nematodes Meloidogyne javanica, M. incognita, and M. arenaria severely
affect several cereals and vegetables. Some nematodes cause damage in association, with fungi such as Fusarium, Verticillium, Rhizoctonia, and Phytophthora spp. causing wilt and root rots in several crops. In association
with bacterial pathogens such as Clavibacter tritici, the nematode Anguina
tritici causes diseases such as spike blight of wheat. Some nematodes such
as Xiphinema, Trichodorus, and Paratrichodorus serve as vectors of viruses
causing serious diseases in tomato, peach, strawberry, raspberry, grapevine,
pea, cowpea, and tobacco. Several nematicides have been developed, and
these broad-spectrum nematicides were highly effective in management of
nematode-related diseases. However, several effective nematicides have
been removed from the marketplace as the result of federal deregulation.
Research work has been intensified in developing nematode-resistant plants
exploiting single-gene resistance. Cultivars with vertical or horizontal resistance have been developed, with more success with vertical resistance. Several biocontrol agents have been identified, but their practical uses in the
field are yet to be demonstrated. All these aspects are described in this chapter.
STRUCTURE OF NEMATODES
Most plant-parasitic nematodes are minute, vermiform animals. Their
bodies are elongated and threadlike (nema in Greek means thread) without any segments. They are cylindrical, tapering at each end especially toward the tail. Nematode females may swell to become spherical. The size of
plant-parasitic nematodes ranges from 0.2 to 10 mm and is commonly 0.5 to
1.5 mm. The basic body structure of nematodes consists of a flexible body
wall that is composed of cuticle, hypodermis, and somatic muscles. The
wall surrounds a tubelike gut, which is made up of esophagus and intestine.

The body cavity between the gut and the body wall is usually regarded as a
pseudocoelom containing a pseudocoelomic fluid (Bird, 1981; Vidhyasekaran, 1993).
The nerve system consists of a nerve ring that encircles the gut usually in
the region of the esophageal isthmus. Several nerves extend anteriorly and
posteriorly. An excretory duct opens to the exterior via an excretory pore.
The gut is an internal tube beginning at the oral opening and ending at the
ventrally placed anus in juveniles and females and at the cloaca in males
(Southey, 1982).
Females usually have a vulva in the midbody region with two reproductive tracts (didelphic) that are often opposed to each other (amphidelphic).
A single reproductive tract (monodelphic) is usually directed anterior to the
vulva (prodelphic), and the vulva is often located more posteriorly. When
only one fully formed reproductive tract is present, it may be opposed by a
pre- or postvulval sac (Southey, 1982). Males usually have a single or paired
testis, seminal vesicle, and vas deferens. The vas deferens opens into the
cloaca, which has a ventrally placed opening. Males usually have a pair of
copulatory spicules that lie in an invagination of the cloacal wall. Sclerotized structures such as a gubernaculum or lateral accessory pieces guide the
spicules.
The females lay eggs after copulation. The young ones are called larvae.
Nematodes typically moult four times to reach the adult stage (Vidhyasekaran, 1993).
PLANT PARASITIC NEMATODES
The important parasitic nematodes are listed here. The crops affected by
these nematodes are given within parentheses.
Awl Nematodes
Dolichodorus spp. (Crops: celery, corn, crucifers, sorghum)
Dolichodorus heterocephalus Cobb (Crops: bean, celery, corn)
Bulb and Stem Nematode, Leaf and Stem Nematode
Ditylenchus dipsaci (Khn) Filipjev (Crops: alfalfa, bean, beet, corn,
oats, potato, rice, rye, tobacco)
Parasitic Nematodes
85
Burrowing Nematode
Radopholus similis (Cobb) Thorne (Crop: corn)
Citrus Nematode
Tylenchulus semipenetrans Cobb (Crops: citrus, grape)
Crimp Nematode
Aphelenchoides besseyi Christie (Crop: rice)
Cyst Nematodes
Globdera pallida (Stone) Mulvey and Stone (Crop: potato)
G. rostochiensis (Wollenweber) Mulvey and Stone (Crop: potato)
G. solanacearum (Miller and Gray) Behrens = G. virginiae (Miller
and Gray) Behrens (Crop: tobacco)
G. tabacum (Lownsbery and Lownsbery) Behrens (Crop: tobacco)
Heterodera spp. (Crops: celery, wheat)
H. avenae Wollenweber (Crops: barley, corn, oats, rye, wheat)
H. carotae Jones (Crop: carrot)
H. cruciferae Franklin (Crop: crucifers)
H. filipjevi (Madzhidov) Stelter (Crop: barley)
H. glycines Ichinohe (Crops: bean, soybean)
H. hordecalis Andersson (Crop: oat, wheat)
H. latipons Franklin (Crops: barley, oats, wheat)
H. schachtii Schmidt (Crops: beet, cotton, crucifers)
H. trifolii Goffart (Crops: alfalfa, beet)
H. zeae Koshy et al. (Crop: corn)
Punctodera chalcoensis Stone et al. (Crop: corn, oat, wheat)
Dagger Nematodes
Xiphinema spp. (Crops: citrus, grape, strawberry)
X. americanum Cobb (Crops: alfalfa, almond, apple, apricot, bean,
corn, cotton, cucurbit, grape, oat, peach, pear, sorghum, strawberry, tobacco, wheat)
X. brevicolle Lordello and daCosta (Crop: mango)
X. diversicaudatum (Micoletzky) Thorne (Crop: rose)
X. index Thorne and Allen (Crop: grape)

X. mediterraneum Lima (Crop: corn)
X. rivesi Dalmasso (Crops: almond, apple, apricot, peach, pear)
X. vuittenezi Luc et al. (Crop: apple, pear)
False Root-Knot Nematodes
Nacobbus aberrans (Thorne and Schuster) Sher (Crops: beet, potato)
N. dorsalis Thorne and Allen (Crop: corn)
Foliar Nematode
Aphelenchoides ritzemabosi (Schwartz) Steiner (Crops: tobacco)
Grass-Root Gall Nematode
Subanguina radiciola (Greeff) Paramonov (Crop: wheat)
Lance Nematodes
Hoplolaimus spp. (Crops: bean, corn)
H. columbus Sher (Crops: soybean, corn, cotton, mango)
H. galeatus (Cobb) Thorne (Crops: corn, soybean)
H. uniformis (Crop: carrot)
Needle Nematodes
Longidorus spp. (Crops: alfalfa, beet, corn, citrus, grape)
L. africans Merny (Crops: cotton, sorghum)
L. breviannulatus Norton and Hoffman (Crop: corn)
Rotylenchulus spp. (Crop: alfalfa)
Pin Nematodes
Paratylenchus spp. (Crops: alfalfa, apple, bean, celery, crucifers, cucurbits, peanut, sorghum)
Paratylenchus hamatus Thorne and Allen (Crops: alfalfa, celery,
cotton)
Pod Lesion Nematode

Tylenchorhynchus brevilineatus Williams = T. brevicadatus Hopper
(Crop: peanut)
Potato Rot Nematode
Ditylenchus destructor Thorne (Crops: beet, potato)
Reniform Nematodes
Rotylenchulus spp. (Crop: sorghum)
Rotylenchulus reniformis Linford and Oliveira (Crop: bean, cotton,
cucurbits, soybean, tobacco)
Ring Nematodes
Criconemella spp. (Crops: corn, cotton, cucurbit, grape, oat, sorghum, wheat)
C. axesta (Fassuliostis and Williams) Luc and Raski (Crop: rose)
C. ornata (Raski) Luc and Raski (Crop: corn, peanut, soybean)
C. xenoplax (Raski) Luc and Raski (Crops: almond, apple, apricot,
peach)
Criconemoides ovantus Raski (Crop: bean)
Nothocriconemella mutabilis (Taylor) Ebsary (Crop: oats)
Root Nematode
Hirschmaniella oryzae (Soltwedel) Lucand Goodey (Crop: rice)
Root Gall Nematode
Subanguina radicicola (Greeff) Paramonov (Crops: barley, rye)
Root-Knot Nematodes
Meloidogyne spp. (Crops: alfalfa, apple, barley, beet, citrus, coffee,
corn, crucifer, cucurbit, grape, oat, pear, potato, rice, rye, sorghum, strawberry, sugarcane, tomato, wheat)
M. arenaria (Neal) Chitwood (Crops: almond, alfalfa, apricot, banana, bean, peanut, peach, soybean, tobacco)
M. artiellia Franklin (Crop: barley)

M. chitwoodi Golden et al. (Crops: alfalfa, barley, corn, oat, wheat)
M. hapla Chitwood (Crops: alfalfa, bean, carrot, peanut, potato,
rose, soybean, tobacco, strawberry)
M. incognita (Kofoid and White) Chitwood (Crops: alfalfa, almond,
apricot, banana, bean, corn, cotton, peach, potato, soybean, tobacco)
M. javanica (Treub) Chitwood (Crops: alfalfa, almond, apricot, banana, bean, peach, peanut, potato, soybean, tobacco, corn)
M. naasi Franklin (Crops: barley, oat, wheat)
Root Lesion Nematodes
Pratylenchus spp. (Crops: alfalfa, apple, barley, bean, beet, carrot,
celery, citrus, corn, cotton, cucurbit, oat, pear, potato, grape, sorghum, soybean, sugarcane, tobacco, wheat)
P. brachyurus (Godfrey) Filipjev and Schuurmans-Stekhoven
(Crops: banana, corn, peanut, potato, tobacco)
P. coffeae (Zimmerman) Schuurmans-Stekhoven (Crops: banana,
peanut, strawberry)
P. crenatus Loof (Crop: corn)
P. goodeyi Sher and Allen (Crop: banana)
P. hexincisus Taylor and Jenkins (Crop: corn)
P. minyus Sher and Allen (Crop: wheat)
P. neglectus (Rensch) Filipjev and Schuurmans-Stekhoven (Crops:
alfalfa, corn)
P. penetrans (Cobb) Filipjev and Schuurmans-Stekhoven (Crops: alfalfa, apple, bean, carrot, corn, peach, pear, potato, rose, strawberry, tobacco)
P. pratensis (de Man) Filipjev (Crops: crucifer, strawberry)
P. reniformia Linford and Oliveira (Crop: banana)
P. scribneri Steiner (Crops: corn, strawberry)
P. thornei Sher and Allen (Crops: corn, oat, wheat)
P. vulnus Allen and Jensen (Crops: almond, apricot, grape, peach,
rose)
P. zeae Graham (Crop: corn)
Seed and Pod Nematode
Ditylenchus destructor Thorne (Crop: peanut)
Seed Gall Nematode

Anguina tritici (Steinbuch) Chitwood (Crops: rye, wheat)
Sheath Nematode
Hemicycliophora spp. (Crops: oats, soybean)
Hemicycliophora arenaria Raski (Crop: citrus)
Sheathoid Nematode
Hemicriconemoides mangiferae Siddiqi (Crop: mango)
Spiral Nematodes
Helicotylenchus spp. (Crops: alfalfa, corn, cotton, cucurbit, oat, sorghum, soybean, sugarcane, tobacco, wheat)
H. dihystera Cobb (Crops: banana, bean)
H. multicinctus Cobb (Crop: banana)
Helicotylenchus nannus Steiner (Crop: rose)
Rotylenchus spp. (Crops: rose, sugarcane)
Scutellonema spp. (Crops: cotton, sugarcane)
S. cavenessi Sher (Crops: peanut)
Stem Nematode
Ditylenchus angustus (Butler) Filipjev (Crop: rice)
Sting Nematodes
Belonolaimus spp. (Crops: celery, corn, crucifers)
B. gracilis Steiner (Crop: peanut, soybean, strawberry)
B. longicaudatus Rau (Crops: bean, carrot, citrus, corn, cotton, cucurbit, oats, peanut, potato, sorghum, soybean, strawberry, tomato)
Stubby-Root Nematodes
Paratrichodorus spp. (Crops: alfalfa, beet, celery, citrus, corn, cotton, potato, sorghum, tobacco, tomato, wheat)
P. christiei (Allen) Siddiqi (Crops: bean, corn)
P. minor (Colbran) Siddiqi (Crops: corn, cucurbit, oat, sorghum)

Trichodorus spp. (Crops: beet, corn, potato, tobacco, tomato)
Stunt Nematodes
Merlinius spp. (Crops: bean, cotton, oat, tobacco)
M. brevidens (Allen) Siddiqi (Crops: barley, wheat)
Quinisulcius acutus (Allen) Siddiqi (Crops: corn, bean, rose)
Tylenchorhynchus spp. (Crops: alfalfa, bean, citrus, cotton, oat, rose,
sorghum, tobacco)
T. claytoni Steiner (Crop: cucurbit)
T. dubius (Butschli) Filipjef (Crops: barley, corn)
T. maximus Allen (Crop: barley)
Testa Nematode
Aphelenchoides arachidis Bos (Crop: peanut)
DISEASE SYMPTOMS
Most nematodes attack the roots of plants. They may be endoparasites
(i.e., they enter the root and lose contact with soil or a large part of their
body will be inside the root tissue) or ectoparasites (i.e., they are free living
in soil and intermittently feed on the epidermis of roots and root hairs near
the root tip). Meloidogyne, Heterodera, Globodera, Pratylenchus, Radopholous, Rotylenchulus, Helicotylenchus, Criconemella, and Paratylenchus
are endoparasites. Paratrichodorus, Trichodorus, and Belonolaimus are
ectoparasites. Some nematodes, called foliage parasites, primarily infect
and damage the above-ground plant parts. These nematodes may survive in
the soil and/or host-plant residues. Under favorable conditions, the parasites
may crawl up the plant and attack young seedlings or mature plants. The
stem nematode Ditylenchus, the seed gall nematode Anguina, and the foliar
nematode Aphelenchoides belong to this category.
Symptoms of nematode-infested plants are mostly nonspecific. Patchy
yellow appearance, dieback symptoms, and slow decline are frequently observed symptoms in the nematode-infested field. Above-ground symptoms
due to root parasites include stunting (poor growth and lack of vigor), yellowing (probably due to nutritional deficiency), wilting, and reduced yield.
Foliage parasites may cause crinkled and distorted stems and leaves. The
growing points of seedlings may be killed by the nematodes (e.g., Anguina
tritici). The infected flower primordium develops into a gall, as in the case
of seed galls caused by A. tritici. The stem-and-bulb nematode Ditylenchus

dipsaci causes necrosis and discoloration in stem and leaves. Lesions and
leaf spots appear due to feeding of Aphelenchoides. Leaf and stem galls are
produced by Anguina millefolii.
Some characteristic below-ground symptoms are induced by root-parasitic nematodes. These include the following:
Root Knots
A root knot is the formation of a hard mass of wood in the root. Galls
(swellings) may also be produced in the infested root. Meloidogyne forms
characterisic galls on the roots of many plants. Ditylenchus, Longidorus,
and Xiphinema may cause gall-like swellings on roots.
Stubby Root
Paratrichodorus induces stubby (short and broad) root, with elongated
swellings on root endings.
Root Lesions
These are typical injuries caused by the penetration and movement of
nematodes through the cortex of the root. The lesion nematode Pratylenchus and the burrowing nematode Radopholus cause root lesions. Small
lesions are sometimes induced by the ring nematode Criconemoides.
Root Rots
Some nematodes may cause extensive damage to fleshy root tissues. This
may be followed by rotting, probably due to a secondary invasion by fungi
and bacteria. Ditylenchus destructor causes rotting of potato and beet roots.
Hairy Root
During feeding, some nematodes kill distal parts of the root by girdling.
The remaining portion of the root that is alive usually develops branch
rootlets. This abnormality is called hairy root, bearding, or witches-broom.
Pratylenchus spp., Naccobus, and Meloidogyne hapla cause this type of
symptom.
Root Surface Necrosis

Some ectoparasites may kill the surface cells of large areas, causing superficial discoloration. Paratrichodorus may cause this symptom.
Devitalized Roots
Due to nematode feeding, the growth of root tips stops even though the
cells are not actually killed. These roots may produce branches, which in
turn stop further growth. Trichodorus causes the production of devitalized
roots.
Curly Tip
Sometimes a root injury caused by nematodes may retard elongation of
root tip and cause it to curl. Dagger nematode (Xiphinema) and root-knot
nematode (Meloidogyne) induce curly tip symptoms.
Coarse Root
Nematode infestation may inhibit the growth of lateral roots, resulting in
an open root system that has main roots without branches. This type of
symptom is called coarse root and is caused by Paratrichodorus and Belanolaimus.
MANAGEMENT OF PARASITIC NEMATODES
Quarantine
Parasitic nematodes can be excluded from nonendemic areas that extend
beyond governmental boundaries where it is possible for quarantine personnel to examine them at a few entry points. Domestic quarantines have been
established in the United States to prevent the spread of the potato cyst
(golden) nematodes since 1941. All movement of plants, soil, etc., from
land still under cultivation on Long Island was rigidly controlled. The
United States Department of Agriculture (U.S.D.A.) Golden Nematode
Control Project prescribed stringent import controls to restrict the spread of
the nematode (Southey, 1982). Most European countries legislated to prevent the spread of the potato cyst nematodes to apparently clean areas and to
prevent multiplication to damaging levels. In the Netherlands, Ditylenchus
dipsaci in flower bulbs was excluded by strict quarantine. The Dutch plant
health regulations require inspection of all bulb crops, and nematode-
infested stocks must be given a prescribed hot-water treatment or be destroyed.

Sanitation
Nematodes do not move more than a few inches per year of their own accord, and most spread is passive. Infested farm implements may spread the
nematodes to neighboring fields. Farm implements should be cleaned before taking them to new fields.
Use of Clean Seed
Some seeds may be contaminated with nematodes. Anguina tritici galls
are often found mixed with wheat seeds. Such seeds should be cleaned and
the galls should be eliminated.
Selection of Healthy Propagating Material
In plants that are vegetatively propagated, it is possible to eliminate nematodes by selecting uninfected plants or parts of plants for propagation. The
banana rhizome rot nematode Radopholus similis spreads through suckers.
Hence, healthy suckers alone should be used for planting. In Florida, a stateoperated certifying program exists. It certifies the citrus planting material as
free from the citrus nematode Tylenchulus semipenetrans to exclude the
nematode from new citrus planting areas of the state (Duncan and Cohn,
1990).
Crop Rotation
Crop rotation may be beneficial in eliminating or reducing some nematode populations in the field. Heterodera, Globodera, and Meloidogyne do
not survive in soil for long, and therefore can be controlled by proper crop
rotation. Aphelenchoides disappears in soil in about two months and hence
no crop rotation is needed to eliminate it. On the other hand, Ditylenchus
dipsaci persists in heavy soil for a long time and crop rotation does little to
reduce its population.
Soil Amendments
Soil amendments are commonly used to control nematodes. Manures,
compost, and oil cakes affect the population levels of plant-parasitic nematodes. Application of organic manures may enhance nematophagus fungi
and antagonistic bacteria, and hence reduce the nematode infestation (Oka
and Yermiyahu, 2002). Application of chitin can also reduce nematode population. A commercial product (Clandosan) containing chitin and urea has
been registered in the United States. Chitin amendments may release ammonia upon degradation at rates that can be nematicidal. Chitin application
may result in a buildup of chitinolytic organisms that may contribute to the
mortality of nematode eggs that contain chitin (Duncan, 1991).
Plants Inhibitory to Nematodes
Root exudates of some plants are inhibitory to nematodes. Tagetes erecta
reduces Pratylenchus infection through nematicidal action. Asparagus reduces Paratrichodorus christiei population. Crotolaria reduces Meloidogyne population. White mustard reduces the potato cyst nematode. These
plants can be grown as an alternate crop or as an intercrop. Intercropping
will be more beneficial.
Trap Cropping
Trap cropping is one of the important components in the integrated nematode management system. It is useful to control Globodera and Heterodera
spp. In this system, a crop that causes the nematodes to hatch is planted; if
the crop is a host plant it must be destroyed before the nematodes mature,
but if the larvae do not develop, the crop can be allowed to mature normally.
Oat is an efficient trap crop for Heterodera avenae, a nematode that attacks
wheat and barley. Oat should be ploughed in before the nematodes mature.
Biological Control
Several antagonistic organisms have been reported to be present in soil.
Several Pseudomonas spp. are antagonistic to the nematodes. Predacious
nematodes are common in soil. A large amount of organic matter is essential
to activate these biological agents for control of nematode infection. The
bacterium Pasteuria penetrans, nematophagus fungi Dactylella, Dactylaria,
and Arthrobotrys, and egg parasites such as Paecilomyces lilacinus are used
for the control of nematodes (Sayre and Walter, 1991). Mixtures of biocontrol agents enhance biological control of Meloidogyne javanica in tomato (Siddiqui and Shukat, 2002).
Physical Methods
Infested soil can be sterilized with steam. Potato cyst nematodes are
killed by passing the steam into the soil through perforated pipes. Hot-water
treatment can be beneficial to control cyst nematodes in potato tubers.
Solarization using plastic mulches may help to reduce the nemetode population (Salch et al., 1988).
Host Plant Resistance
Several resistant crop varieties have been developed against Ditylenchus,
Meloidogyne, Heterodera, and Globodera spp. using both dominant major
gene (qualitative) resistance and polygenic (quantitative) resistance. Resistance in most of the developed resistant varieties is conferred by dominant
major genes. Major gene resistance may be more durable against nematodes
than against fungal and bacterial pathogens because nematodes disperse slowly
and reproduce at relatively low numbers. Hence, several nematode-resistant
varieties have been developed incorporating a single resistance gene (Duncan, 1991). Some genes confer resistance to more than one species of nematode. A gene in tomato, Mi, confers resistance to Meloidogyne incognita, M.
javanica, and M. arenaria. This gene has been incorporated into a large
number of tomato varieties with good agronomic characteristics. Growers
in California choose to use these resistant varieties rather than other means
to manage the nematodes (Duncan, 1991). The resistance gene Mi in tomato
has been cloned (Rossi et al., 1998), and another gene, Mi-9, has been characterized (Ammiraju et al., 2003).
Chemical Control
Many fumigants are available to eradicate nematodes. Methyl bromide,
dichloropropene, dibromochloropropane, and metham-sodium can be injected into the soil to eradicate nematodes. Some of the granules are known
to reduce the nematode population. Aldicarb, oxamyl, and carbofuran can
also be useful to reduce the nematode infestation. The following are the
nematicides available for management of nematodes.
Aldicarb (2-methyl-2-(methylthio) propionaldehyde Omethylcarbamoyloxime); Temik (trade name); granules; used to control
free-living nematodes
Chloropicrin (trichloronitromethane); Pic-Clor, Chlor-O-Pic (trade
names); preplant soil fumigant
Carbofuran (2,3-dihydro-2,2-dimethylbenzofuran-7-yl-methylcarbamate);
Furadan (trade name); granules and flowable formulations; soil application
D-D (1,2-dichloropropane with 1,3-dichloropropene); Vidden D (trade
name); soil-injected nematicide; mixed formulations: D-D + methyl
isothiocyanate, D-D + chloropicrin
Dazomet (tetrahydro-3.5-dimethyl-1,3,5-thiadiazine-2 thione); Basamid,
Mylone (trade names); soil sterilant; used as preplant treatment in tobacco, turf seedbeds
Diazinon (O,O-diethyl O-2-isopropyl-6-methylpyrimidin-4-yl
phosphorothioate); Diazol, Basudin, Neocidol, Exodin, Diazitol,
Spectracide, Sarolex, Diagran (trade names); granules; effective against
many nematodes
Dibromochloropropane (1,2-dibromochloropropane); Nemagon,
Fumazone (trade names); emulsifiable concentrate and nonemulsifiable
concentrate; nematicidal soil sterilant used on citrus, grapes, peanuts,
deciduous fruit, cotton, and vegetables
Dichlofenthion (O-(2,4-dichlorophenyl) O,O-diethyl phosphorothioate);
Mobilawn, VC 13 Nemacide (trade names); nonsystemic nematicide;
controls noncyst-forming nematodes on turf and ornamentals; mixed
formulation: dichlofenthion + thiram
Dichloropropene (1,3-dichloropropene); Telone (trade name); liquid formulation; nematicidal control in fruit, flower, and vegetable crops,
cotton, soybean, and peanut; effective against potato cyst nematode,
root-knot nematode in cucumbers and tomato, and stem nematode in
strawberry
Ethoprophos (O-ethyl S, S-dipropyl phosphorodithioate); Mocap, Prophos
(trade names); granules and emulsifiable concentrate formulations;
nonsystemic nematicide; controls nematodes in potato
Ethylene dibromide (1,2-dibromoethane); Dowfume, Bromofume,
Soilbrom, Soilfume (trade names); liquid formulation; mixed formulations: Ethylene dibromide + chloropicrin, ethylene + methyl bromide
Fenamiphos (ethyl-4-methylthio-m-tolyl isopropylphosphoramidate);
Nemacur (trade name); granules and emulsifiable concentrate; systemic nematicidal action; absorbed through leaves and roots and controls ecto- and endo-parasitic nematodes; applied broadcast, in band,
in-the-row, by drench before or at planting time or to established plants
Fensulfothion (O,O-diethyl O-4-methylsulphinylphenyl
phosphorothioate); Dasanit, Terracur P (trade names); systemic and
contact action; soil application for nematicidal control of parasitic, sedentary, and free-living nematodes
Metham-sodium (sodium methyldithiocarbamate); Vapam, VPM,

Trimaton, Maposol, Sistan (trade names); soil fumigant applied prior to
planting edible crops
Methyl bromide (bromomethane); Haltox, Terabol, Meth-O-Gas,
Dowfume, Brom-O-Gaz, Bercema (trade names); soil fumigant
Methyl isothiocyanate (isothiocyanatomethane); Trapex (trade name); soil
fumigant
Oxamyl (N,N-dimethyl-2-methylcarbamoyloxyimino-2-(methylthio)
acetamide); Vydate (trade name); granules and water-soluble liquid
formulations; systemic contact nematicide; uptake through leaves and
roots, transported in plants downward to the root
Terbufos (S-tert-butylthiomethyl O,O-diethyl phosphorodithionate);
Counter (trade name); granules
Thionazin (O-O-diethyl-O-pyrazin-2 yl-phosphorothionate); Nemafos,
Zinophos, Cynem, Nemaphos (trade names); granules, emulsifiable
concentrate formulations; controls free-living and plant-parasitic nematodes
Some nematodes act as vectors of viruses that cause serious diseases in
crops. They are described in Chapter 10.
REFERENCES
Ammiraju, J. S. S., Veremis, J. C., Huang, X., Roberts, P. A., and Kaloshian, I.
(2003). The heat-stable root-knot nematode resistance gene Mi-9 from Lycopersicon peruvianum gene is localized on the short arm of chromosome 6. Theor
Appl Genet, 106:478-484.
Bird, A. F. (1981). The Structure of Nematodes. Academic Press, London.
Duncan, L. W. (1991). Current options for nematode management. Annu Rev
Duncan, L. W. and Cohn, E. (1990). Nematode parasites of citrus. In M. Luc, R. A.
Sikora, and J. Bridge (Eds.), Plant Parasitic Nematodes in Subtropical and
Tropical Agriculture, CAB Institute, Wallingford, pp. 321-346.
Oka, Y. and Yermiyahu, U. (2002). Suppressive effects of composts against the
root-knot nematode Meloidogyne javanica on tomato. Nematology, 4:891-898.
Rossi, M., Goggin, F. L., Milligan, S. B., Kolashian, I., Ullman, D. E., and Williamson, V. M. (1998). The nematode resistance gene Mi of tomato confers resistance against the potato aphid. Proc Natl Acad Sci USA, 95:9750-9754.
Salch, H., Abu-Gharbich, W. I., and Al-Banna, L. (1988). Effect of solarization
combined with solar-heated water on Meloidogyne javanica. Nematologica,
34:290-291.
Sayre, R. M. and Walter, D. E. (1991). Factors affecting the efficacy of natural enemies of nematodes. Annu Rev Phytopathol, 29:149-166.
Siddiqui, I. A. and Shukat, S. S. (2002). Mixtures of plant disease suppressive bacteria enhance biological control of multiple tomato pathogens. Biol Fertil Soils,
36:260-268.
Southey, J. F. (1982). Plant Nematology. Her Majestys Stationery Office, London.
Vidhyasekaran, P. (1993). Principles of Plant Pathology. CBS Publishers.
Williams, T. D. (1984). Plant parasitic nematodes. In The Commonwealth Mycological Institute (compilation), Plant Pathologists Pocketbook. Common Mycological Institute, Kew, England, pp. 119-136.
Phytoplasmas
Phytoplasmasand
and Spiroplasmas
Spiroplasmas
Phytoplasmas and spiroplasmas are bacteria that lack rigid cell walls.
They belong to the class Mollicutes. Phytoplasmas cannot be cultured,
whereas some spiroplasmas can be cultured. Both mollicutes are phloem
limited in plants and can be transmitted by leafhoppers. Spiroplasmas are
helical in shape. The structure of phytoplasmas and spiroplasmas, classification of phytoplasmas based on their 16S rRNA gene sequences, and diseases caused by them are described.
PHYTOPLASMAS
What Are Phytoplasmas?
Phytoplasmas are minute bacteria without cell walls. They inhabit phloem
sieve elements in infected plants. They cannot be cultured in artificial media
to date. They can pass through a bacteria-proof filter. They have been associated with diseases in cereals, vegetables, fruit crops, ornamental plants,
and timber and shade trees.
Yellows are an important group of diseases and were once considered to
be caused by viruses. One disease, aster yellows, was first reported in 1902,
and it was considered a virus disease until 1967. Doi et al. (1967) reported
that particles in ultrathin sections of the phloem of plants affected by yellows diseases, including aster yellows, resembled animal and human mycoplasmas. The particles lacked rigid cell walls, were surrounded by a single
unit membrane, and were sensitive to the antibiotic, tetracycline. The term
mycoplasmalike organisms (MLOs) was used to refer to such causal organisms of yellows diseases from 1967 to 1994. The name phytoplasma was
introduced by the Phytoplasma Working Team at the Tenth Congress of the
International Organization for Mycoplasmology (Lee et al., 2000).
Classification of Phytoplasmas
Despite several attempts during the past three decades, phytoplasmas
have not been cultured. Hence, phytoplasmas could not be classified based
on the traditional tests applied to cultured prokaryotes. Woese et al. (1980)
distinguished phytoplasmas (mycoplasmas) by analyzing highly conserved
rRNA gene sequences in prokaryotes. It has been suggested that the phytopathogenic, mycoplasmalike organisms belong to the class Mollicutes. Four
major phylogenetic groups (clades) have been identified in the class Mollicutes: Mycoplasma hominis, M. pneumoniae, Spiroplasma, and Anaeroplasma. Phylogenetic analyses of 16S rRNA and rp (ribosomal proteins)
gene operon sequences showed that phytoplasmas formed a large, discrete,
monophyletic clade within the expanded Anaeroplasma clade (Gundersen
et al., 1994). Within the phytoplasma clade, several distinct subclades
(monophyletic groups or taxa) have been identified based on the restriction
fragment length polymorphism (RFLP) of 16S rRNA and ribosomal protein
sequences (Lee et al., 2000). It has been suggested that the phytoplasma
clade should be distinguished at the taxonomic level of a genus, and each
subclade (corresponding 16S rRNA sequence) should represent a species.
However, the naming of new species in the class Mollicutes requires descriptions of the species in pure culture. Phytoplasmas cannot be isolated in
pure culture, and the phenotypic characteristics used to describe mollicute
species are unattainable for uncultured phytoplasmas. Therefore, a provisional classification system using the Candidatus category has been developed (Lee et al., 2000). Five Candidatus Phytoplasma species have been
recognized. The following is the classification of phytoplasmas based on
RFLP analysis of 16S rRNA and ribosomal protein sequences (Seemuller et
al., 1998; Lee et al., 2000):
Aster Yellows Group (16 SrI)
Subgroup 16SrI-A, 16SrI-A (rp-A): aster yellows (in China aster),
lettuce yellows, periwinkle little leaf, tomato big bud (Arkansas),
gladiolus virescence (Italy)
Subgroup 16SrI-B, 16SrI-B (rp-B): aster yellows (in potato, carrot,
celery, clover), broccoli phyllody, chrysanthemum yellows, cabbage witches-broom, onion virescence (yellows), mulberry
dwarf, eggplant dwarf, turnip virescence
Subgroup 16SrI-B, 16Sr-B (rp-K): hydrangea phyllody
Subgroup 16SrI-B, 16Sr-B (rp-L): maize bushy stunt
Subgroup 16SrI-C: strawberry green petal, clover phyllody, olive
witches-broom
Subgroup 16SrI-D: paulownia witches-broom

Subgroup 16SrI-E: blueberry stunt
Subgroup 16SrI-F: apricot chlorotic leaf roll
Subgroup 16SrI-K: strawberry multiplier
Peanut Witches-Broom Group (16SrIII)
Subgroup 16SrII-A: peanut witches-broom, sweet potato witchesbroom, sunn hemp witches-broom
Subgroup 16SrII-B (Candidatus Phytoplasma aurantifolia): lime
witches-broom
Subgroup 16SrII-C: cotton phyllody, soybean phyllody, faba bean
phyllody
Subgroup 16SrII-D: sweet potato little leaf
Subgroup 16SrII-E (Candidatus Phytoplasma australasia): papaya
mosaic, papaya yellow crinkle, tomato big bud
X-Disease Group (16SrIII)
Subgroup 16SrIII-A: peach X-disease, cherry X-disease
Subgroup 16SrIII-B: clover yellow edge (Canada), italian clover
phyllody
Subgroup 16SrIII-C: pecan bunch
Subgroup 16SrIII-D: goldenrod yellows
Subgroup 16SrIII-E: spirea stunt
Subgroup 16SrIII-F: milkweed yellows
Subgroup 16SrIII-G: walnut witches-broom
Subgroup 16SrIII-H: poinsettia branching inducing
Coconut Lethal Yellows Group (16SrIV)
Subgroup 16SrIV-A: coconut lethal yellows
Subgroup 16SrIV-B: tanzanian coconut lethal decline
Elm Yellows Group (16SrV)
Subgroup 16SrV-A, 16SrV-A (rp-A): alder yellows, elm yellows,
elm witches-broom, rubus stunt
Subgroup 16SrV-B, 16SrV-B (rp-B): cherry lethal yellows
Subgroup 16SrV-B, 16SrV-B (rp-C): jujube witches-broom
Subgroup 16SrV-C, 16SrV-A (rp-D): flavescence doree (grapevine)
Clover Proliferation Group (16SrVI)

Subgroup 16SrVI-A: potato witches-broom, alfalfa witchesbroom, tomato big bud (California), clover proliferation
Subgroup 16SrVI-B: strawberry multiplier (Canada)
Unclassified: brinjal little leaf, willow witches-broom
Ash Yellows Group (16SrVII)
Subgroup 16SrVII-A (Candidatus Phytoplasma fraxini): ash yellows, lilac witches-broom
Loofah Witches-Broom Group (16SrVIII)
Subgroup 16SrVIII-A: loofah witches-broom
Pigeon Pea Witches-Broom Group (16SrIX)
Subgroup 16SrIX-A: pigeon pea witches-broom
Unclassified: gliricidia little leaf, picris phyllody
Apple Proliferation Group (16SrX)
Subgroup 16SrX-A: apple proliferation, hazel decline
Subgroup 16SrX-B: apricot chlorotic leaf roll, plum leptonecrosis,
European stone fruit yellows
Subgroup 16SrX-C: pear decline, peach yellow leaf roll
Rice Yellow Dwarf Group (16SrXI)
Subgroup 16SrXI-A: rice yellow dwarf
Subgroup 16SrXI-B: sugarcane grassy shoot, sugarcane whiteleaf
Stolbur Group (16SrXII)
Subgroup 16SrXII-A: pepper stolbur, tomato stolbur, grapevine yellows (bois noir), celery yellows
Subgroup 16SrXII-B (Candidatus Phytoplasma australiense): Australian grapevine yellows, papaya dieback)
Candidatus Phytoplasma japonicum: Japanese hydrangea phyllody
Mexican Periwinkle Virescence Group (16SrXIII)

Subgroup 16SrXIII-A: Mexican periwinkle virescence
Subgroup 16SrXIII-B: strawberry green petal
Bermudagrass White Leaf Group (16SrXIV)
Subgroup 16SrXIV-A: bermudagrass white leaf
Symptoms of Phytoplasma Diseases
Phytoplasma diseases are characterized by several types of symptoms.
The important symptoms are:
Phyllodyfloral parts transformed into green leafy structures
Virescencethe development of green flowers and loss of normal
flower pigments
Witches-broomproliferation (mass outgrowths) of the branches of
woody plants; proliferation of auxiliary shoots
Yellowsuniform yellow to almost white discoloration of leaves;
yellowing will be the conspicuous symptom in the diseased plant
Stuntingretardation of plant growth, small flowers, and leaves;
shortened internodes
Sterilitysuppressed development of reproductive structures
Leaf curlingthe distortion, puffing, and crinkling of a leaf resulting from the unequal growth of its two sides
Bushy stuntdiseased plant severely stunted; its shoots are
crowded, giving bushy appearance
Little leafin the diseased plants, the leaves are malformed into
tiny chlorotic structures
Leaf rollplant leaves tend to curl
Big budthe fruit bearing shoots become thick, dark green structures; fruits formed in infected plants become hard and woody
Diebackdeath of twigs from the tip backward
Slender shootsabnormal elongation of internodes resulting in
slender shoots
Reddeningpredomination of red pigments
Blackeningblackening of tissues due to intensive necrosis
Transmission and Mode of Spread
Phytoplasmas are phloem-limited pathogens that are found in phloem
sieve elements. Phytoplasmas cannot be transmitted mechanically. Sap-
sucking insects such as leafhoppers and planthoppers can transmit phytoplasmas. These insects feed on phloem tissues, where they acquire phytoplasmas
and transmit them from plant to plant. Phytoplasmas may overwinter in
infected vectors. They may survive in perennial plants. No seed-borne phytoplasmas have been reported. However, phytoplasmas are spread by vegetative propagation through cuttings, storage tubers, rhizomes, or bulbs.
They can be transmitted also through grafts.
Disease Diagnosis
Because phytoplasmas cannot be cultured, their detection is difficult. The
presence of characteristic symptoms in diseased plants and subsequent observation of phytoplasma bodies in ultrathin sections were used to diagnose
phytoplasma diseases. Recently, several molecular probes have been developed to diagnose these diseases. Enzyme-linked immunosorbent assays,
immunofluorescence microscopy, DNA probes, Southern hybridization and
RFLP analysis of phytoplasma genomic DNA, and PCR assays using PCR
primers based on sequences of cloned phytoplasma DNA fragments have
been used to detect phytoplasma diseases (see Chapter 13 for details about
these techniques). The most useful method is the PCR method, which employs phytoplasma universal (generic) or phytoplasma group-specific oligonucleotide primers that are based on highly conserved 16S rRNA gene sequences.
Phytoplasma Disease Management
Disease-free planting material should be used for planting. Diseaseresistant varieties are available for protection against some diseases. Insect
vectors should also be controlled. Genetic engineering technology has been
exploited to develop transgenic plants that are resistant to phytoplasma. The
expression of antibodies in transgenic plants confers resistance to phytoplasma diseases (Chen and Chen, 1998).
SPIROPLASMAS
Spiroplasmas are wall-less prokaryotes that belong to the class Mollicutes (Kirkpatrick and Smart, 1995). They are helical prokaryotes that lack
a rigid cell wall. Unlike phytoplasmas, some spiroplasmas can be cultured
(Daniels, 1983). Three plant-pathogenic spiroplasmas have been identified:
Spiroplasma citri, the causal agent of citrus stubborn and horseradish
brittleroot; S. kunkelii, the causal agent of corn stunt disease; and S. phoen-
icium, a spiroplasma isolated from diseased periwinkle plants (Kirkpatrick

and Smart, 1995). Spiroplasma citri can be cultured, whereas S. kunkelli
cannot. Spiroplasmas, in common with phytoplasmas, are found only in
phloem sieve tubes (Bove, 1984). Spiroplasma citri has a genome size close
to 109 Daltons (Bove, 1984). The spiroplasma has been shown to contain
three DNA polymerases. Spiroplasmas carry only one (16S-rRNA) or two
rRNA operons (16S-23S-5 S rRNA), whereas bacteria such as Escherichia
coli and Bacillus subtilis have seven to ten rRNA operons.
Spiroplasma citri may be transmitted by the beet leafhopper Circulifer
tenellus. Spiroplasma kunkelii is transmitted by the corn leafhopper Dalbulus maidis and certain other Dalbulus and Baldulus species. Spiroplasmas can infect and multiply within the insects. Spiroplasmas can be detected in many organs and tissues of infected insects. Spiroplasmas pass
from the insect gut lumen into the blood stream, passing through the endoplasmic reticulum. However, the transmission of spiroplasmas by insects
has not been convincingly demonstrated.
Important Diseases Caused by Spiroplasmas
Citrus stubborn: Spiroplasma citri has a wide host range. At least 15 families of wild dicotyledonous and one family of monocotyledonous plants
have been recorded as hosts (Daniels, 1983).
Corn stunt: Spiroplasma kunkelli causes dwarfing of the plant, chlorotic,
distorted leaf margins, loss of apical dominance, and reduced flower size.
However, infected corn plants do not wilt and die.
REFERENCES
Bove, J. M. (1984). Wall-less prokaryotes of plants. Annu Rev Phytopathol, 22:361396.
Chen, Y. D. and Chen, T-A. (1998). Expression of engineered antibodies in plants:
A possible tool for spiroplasma and phytoplasma disease control. Phytopathology, 88:1367-1371.
Daniels, M. J. (1983). Mechanisms of spiroplasma pathogenicity. Annu Rev Phytopathol, 21:29-43.
Doi, Y. M., Teranaka, M., Yora, K., and Asuyama, H. (1967). Mycoplasma or PLTgroup-like microorganisms found in the phloem elements of plants infected with
mulberry dwarf, potato witches broom, aster yellows, or paulownia witches
broom. Ann Phytopathol Soc Jpn, 33:259-266.
Gundersen, D. E., Lee, I.-M., Rehner, S. A., Davis, R. E., and Kingsbury, D. T.
(1994). Phylogeny of mycoplasmalike organisms (phytoplasmas): A basis for
their classification. J Bacteriol, 176:5244-5254.
Kirkpatrick, B. C. and Smart, C. D. (1995). Phytoplasmas: Can phylogeny provide

the means to understand pathogenicity? Adv Bot Res, 21:188-212.
Lee, I-M., Davis, R. E., Gundersen-Rindal, D. E. (2000). Phytoplasma: Phytopathogenic mollicutes. Annu Rev Microbiol, 54:221-255.
Seemuller, E., Marcone, C., Lauer, U., Ragozzino, A., and Goschl, M. (1998). Current status of molecular classification of the phytoplasmas. J Plant Pathol, 80:
3-26.
Woese, C. R., Maniloff, J., and Zablen, L. B. (1980). Phylogenetic analysis of the
mycoplasmas. Proc Natl Acad Sci USA, 77:494-498.
Viroids
Viroids
Viroids are covalently closed, circular RNA molecules (Hammond and
Zhao, 2000). Viroids were the first circular RNAs to be discovered in nature.
These are the smallest known infectious agents (Elena et al., 2001). Potato
spindle tuber viroid was the first viroid reported, and it is widely prevalent
in different potato growing areas. Citrus exocortis viroid is widespread in
citrus production areas where trifoliate orange (Poncirus trifoliate) is used
as root stock (Guo et al., 2002). Hop stunt viroid has a wide range of hosts.
Mechanism of viroid pathogenesis in plants has been elucidated recently.
This chapter describes the viroids and viroid-like satellite RNAs (virusoids).
STRUCTURE OF VIROIDS
Viroids are nucleic acids that exist naturally with no protein coat. They
consist of ribonucleic acid (RNA). These miniviruses are the smallest
known causal organisms of infectious diseases. They are subviral, and their
size ranges from 246 to 388 nucleotides in length (Symons, 1991). The RNA
structure of viroids is different from transfer RNA (tRNA), ribosomal RNA
(rRNA), and messenger RNA (mRNA). Viroids were the first circular
RNAs to be discovered in nature; this feature differentiates them from other
RNAs (Reisner, 1991).
Important Viroids Causing Diseases
The following are viroids that cause diseases in important crops:
Apple scar skin viroid
Australian grapevine viroid
Avocado sunblotch viroid
Chrysanthemum chlorotic mottle viroid
Chrysanthemum stunt viroid
Citrus exocortis viroid
Coconut cadang-cadang viroid
Coconut tinangaja viroid
Columnea pale fruit viroid

Cucumber pale fruit viroid
Grapevine viroid 1B
Grapevine yellow speckle viroid
Hop latent viroid
Hop stunt viroid
Potato spindle tuber viroid
Tomato apical stunt viroid
Tomato planta macho viroid
VIROID INFECTION PROCESS AND MANAGEMENT
Infection Process
The viroid RNA does not code for any genes. Viroid replication and
pathogenesis may depend completely on the enzyme systems of the host.
The viroid RNA is dependent upon the host for its replication as well as
intraplant movement. The functions necessary for propagation of the viroids are derived completely from the host. The viroids are associated with
and replicate in either the nucleus or the chloroplasts of the plants (Zaitlin
and Palukaitis, 2000). They are replicated by host-encoded RNA polymerases (Fels et al., 2001). They do not encode proteins. Viroids replicate
within the nucleus of infected cells without a helper virus. Viroids are transported into the plant nucleus, and typically potato spindle tuber viroid
(PSTV) possesses a sequence and/or structural motif for nuclear transport
(Woo et al., 1999). Phloem proteins may be involved in systemic transport
of viroids in the plants (Gomez and Pallas, 2001). Phloem protein 2, a
dimeric lectin, is the abundant component of phloem exudates of cucumber.
This protein interacts with the viroid RNA and facilitates the systemic
movement of hop stunt viroid (Owens et al., 2001).
Symptoms
Infection with viroids does not result in obvious macroscopic symptoms.
Common symptoms of viroid diseases include retardation of plant growth
and stunting. Potato plants infected with the potato spindle tuber viroid are
smaller than healthy plants. However, tuber symptoms are prominent. The
diseased tubers are spindle shaped. Citrus trees infected with the Citrus
exocortis viroid are stunted. Symptoms of the disease include scaling of the
bark below the graft union. Stunted trees crop well for their size, and the
fruit is normal (Diener, 1979). Stunting is the important symptom of tomato

plants affected by the tomato bunchy top viroid, hop plants infected by the
hop stunt viroid, and chrysanthemum plants infected by the chrysanthemum
stunt viroid and the chrysanthemum chlorotic mottle viroid.
Mode of Spread
Viroids are highly contagious and mechanically transmitted. They are
spread by leaf contact. Viroids are spread also by contaminated planting and
cultivating equipments. They may be disseminated mostly as a result of cultural operations through contaminated knives, tools, and hands. Some reports indicate that viroids are transmitted by insects. Potato spindle tuber
viroid has been reported to be transmitted by aphids (Myzus persicae and
Macrosiphum euphorbiae), grasshoppers (Melanoplus spp.), flea beetles
(Epitrix cucumeris and Systena taeniata), tarnished plant bugs (Lygus
pratensis), larvae of the Colorado potato beetle (Leptinotarsa decemlineata), and the leaf beetle (Disonycha triangularis). However, the transmission of viroids by insects is negligible, and mechanical transmission is more
important.
Viroid Disease Management
Because viroids are spread mechanically, disease-free planting materials
should be used for planting. Cutting knives and all planting and field equipment should be cleaned scrupulously. Commercial cultivars with high resistance to the diseases are lacking.
VIRUSOIDS (ENCAPSIDATED, VIROIDLIKE,
SATELLITE RNAS)
Some viruses contain a viroidlike satellite RNA in addition to a linear,
single-stranded molecule of genomic RNA. Such viroid-like satellite RNAs
are called virusoids. They show little sequence homology with viroids, but
they do show significant homology with the linear satellite RNA associated
with Tobacco ringspot virus. The virusoids in infected plants exist almost
solely as circular molecules, either free or encapsidated within virion of the
helper virus. This is in contrast to similarly sized satellite RNA of the Tobacco ringspot virus that is found as both linear and circular single-stranded
molecules in vivo, but only the linear form is encapsidated (Symons, 1991).
Virusoids do not code for any polypeptides.
Only four virusoids have been discovered (Symons, 1991). They are
found with the helper viruses, Lucerne transient streak virus (virusoid name
abbreviation: vLTSV, 324 nucleotides in length), Solanum nodiflorum mottle virus (vSNMV, 377 nucleotides in length), Subterranean clover mottle
virus (vSCMoV, 332 and 388 nucleotides in length), and Velvet tobacco
mottle virus (vVTMoV, 365 and 366 nucleotides in length).
REFERENCES
Diener, T. (1979). Viroids and Viroid Diseases. John Wiley & Sons, New York.
Elena, S. F., Dopazo, J., Pena, M. de la., Flores, R., Diener, T. O., and Moya, A.
(2001). Phylogenetic analysis of viroid-like satellite RNAs from plants: A reassessment. J Mol Evol, 53:155-159.
Fels, A., Hu, K. H., and Riesner, D. (2001). Transcription of potato spindle tuber
viroid by RNA polymerase II starts predominantly at two specific sites. Nucleic
Acids Res, 29:4589-4597.
Gomez, G. and Pallas, V. (2001). Identification of an in vitro ribonucleoprotein
complex between viroid RNA and a phloem protein from cucumber plants. Mol
Plant-Microbe Interact, 14:910-913.
Guo, W. W., Cheng, Y. J., and Deng, X. X. (2002). Regeneration and molecular
characterization of intergeneric somatic hybrids between Citrus reticulata and
Poncirus trifoliata. Plant Cell Rep, 20:829-834.
Hammond, R. W. and Zhao, Y. (2000). Characterization of tomato protein kinase
gene induced by infection by potato spindle tuber viroid. Mol Plant-Microbe Interact, 13:903-910.
Owens, R. A., Blackburn, M., and Ding, B. (2001). Possible involvement of the
phloem lectin in long-distance viroid movement. Mol Plant-Microbe Interact,
14:905-909.
Reisner, D. (1991). Viroids: From thermodynamics to cellular structure and function. Mol Plant-Microbe Interact, 4:122-131.
Symons, R. H. (1991). The intriguing viroids and virusoids: What is their information content and how did they evolve? Mol Plant-Microbe Interact, 4:111-121.
Woo, Y. M., Itaya, A., Owens, R. A., Tang, L., Hammond, R. W., Chou, H. C., Lai,
M. M. C., and Ding, B. (1999). Characterization of nuclear import of potato spindle tuber viroid RNA in permeabilized protoplasts. Plant J, 17:627-635.
10
Viruses
Viruses
Viruses are important groups of plant pathogens. Several viruses are
known to cause crop diseases, and 977 plant virus species have been officially or provisionally recognized (Fauquet and Mayo, 1999). Rice tungro
virus disease causes heavy losses in rice grown in different countries in Asia
(Azzam and Chancellor, 2002). Citrus tristeza virus disease has caused the
decline and death of close to 40 million trees grafted on sour orange
rootstocks in Spain and several million trees in other growing areas in the
Americas, Australia, and South Africa (Terrada et al., 2000). Tomato spotted wilt virus causes significant yield losses in many food and ornamental
crops (Garcia et al., 2000). It was difficult to classify plant viruses into class,
order, family, genus, and species due to lack of knowledge of genetic relationships between viruses. Recently viruses have been grouped into genera,
based on molecular biological studies. A few families and an order have
been created. The virus infection process is now studied at the molecular
level, and the viral genes involved in the pathogenicity have been characterized. Insects, mites, fungi, protozoa, and nematodes serve as vectors of
viruses, and studies of their interaction help to develop integrated virus disease management technology. All these aspects are described in this chapter.
STRUCTURE OF VIRUSES
Viruses are infectious agents that are not visible under the microscope
(i.e., they are submicroscopic) and are small enough to pass through a bacterial filter. Viruses do not have a metabolism of their own and depend upon
living host cells for multiplication. They have only one type of nucleic acid,
either ribonucleic acid or deoxyribonucleic acid. Nucleic acids may be single stranded (ss) or double stranded (ds), and may be single segmented or
with a few segments. The nucleic acids are enclosed in a coat of protein.
Some viruses may have an extra coat (envelope). The number of coat protein polypeptides may range from one to seven. The virus particles are of
different shapes and sizes. They may be elongated (rod shaped), as in the
case of Tobacco mosaic virus; isometric (Cowpea mosaic virus); bacilliform (Alfalfa mosaic virus, Rice tungro bacilliform virus); spherical (Rice
tungro spherical virus); or geminate (i.e., arranged in pairs). Some viruses
have different particle types that differ in size or nucleic acids. Multipartite
(multicomponent, multigenome segments) viruses consist of two to four
single-stranded (ss) RNAs. Two RNA pieces occur in Tobacco rattle virus,
Bean pod mottle virus, and Pea enation mosaic virus; three RNA pieces occur in Cucumber mosaic virus and Prune dwarf virus; and four RNA pieces
occur in Alfalfa mosaic virus and Tomato spotted wilt virus. All RNA pieces
are needed for multicomponent virus infection.
The particle shape and size of important characterized viruses, number of
genome segments, nature of RNA or DNA, and number of coat protein
polypeptides of these viruses are provided here:
Alfalfa mosaic virus (AMV)Bacilliform, 28-58 nm 18 nm, 4 genome
segments, linear ssRNA, 1 coat protein polypeptide
Andean potato latent virus (APLV)Isometric, about 29 nm, 1 genome
segment, linear ssRNA, 1 coat protein polypeptide
Andean potato mottle virus (APMV)Isometric, about 30 nm, 2 genome
segments, linear ssRNA, 2 coat protein polypeptides
Arabis mosaic virus (ArMV)Isometric, about 30 nm, 2 genome
Barley stripe mosaic virus (BSMV)Rigid rod, 100-150 nm 20 nm,
3 genome segments, linear ssRNA, 1 coat protein polypeptide
Barley yellow dwarf virus (BYDV)Polyhedral, about 30 nm, 1 genome
Barley yellow mosaic virus (BaYMV)Flexuous rod, 680-900 nm 11
nm, 1 genome segment, linear ssRNA, 1 coat protein polypeptide
Barley yellow striate mosaic virus (BYSMV)Bacilliform, 330 45 nm,
1 genome segment, linear ssRNA, 5 coat protein polypeptides
Bean common mosaic virus (BCMV)Flexuous rod, 680-900 nm 11 nm,
1 genome segment, linear ssRNA, 1 coat protein polypeptide
Bean golden mosaic virus (BGMV)Geminate (arranged in pairs),
18 30 nm
Bean pod mottle virus (BPMV)Isometric, about 30 nm, 2 genome segments, linear ssRNA, 2 coat protein polypeptides
Bean rugose mosaic virus (BRMV)Isometric, about 30 nm, 2 genome
Bean yellow mosaic virus (BYMV)Flexuous rod, 680-900 nm 11 nm,
Beet leaf curl virus (BLCV)Bacilliform, 225-350 nm 45 nm,
Beet mosaic virus (BtMV)Flexuous rod, 680-900 nm 11 nm,

Beet western yellows virus (BWYV)Polyhedral, about 30 nm,
Beet yellow stunt virus (BYSV)Flexuous rod, 600-2,000 nm 12 nm,
Beet yellows virus (BYV)Flexuous rod, 600-2000 nm 12 nm,
Black raspberry latent virus (BRLV)Isometric, 26-35 nm, 3 genome
Broad bean stain virus (BBSV)Isometric, about 30 nm, 2 genome
Broad bean true mosaic virus (BBTMV)Isometric, about 30 nm,
2 genome segments, linear ssRNA, 2 coat protein polypeptides
Brome mosaic virus (BMV)Isometric, about 29 nm, 3 genome
Carnation Italian ringspot virus (CIRSV)Isometric, about 34 nm,
Carnation latent virus (CLV)Flexuous rod, 600-700 nm 13 nm,
Carnation necrotic fleck virus (CNFV)Flexuous rod, 600-2000 nm
12 nm, 1 genome segment, linear ssRNA, 1 coat protein polypeptide
Carnation ringspot virus (CRSV)Isometric, about 34 nm, 2 genome
Carnation vein mottle virus (CVMV)Flexuous rod, 680-900 nm 11 nm,
Carrot latent virus (CaLV)Bacilliform, 220 70 nm, 1 genome
segment, linear ssRNA, 5 coat protein polypeptides
Carrot red leaf virus (CaRLV)Polyhedral, 25-30 nm, 1 genome
Carrot thin leaf virus (CTLV)Flexuous rod, 680-900 nm 11 nm,
Carrot yellow leaf virus (CYLV)Flexuous rod, 600-2000 nm 12 nm,
Cauliflower mosaic virus (CaMV)Isometric, 42-50 nm, 1 circular
genome segment, dsDNA, 1 coat protein polypeptide
Celery mosaic virus (CeMV)Flexuous rod, 680-900 nm 11 nm, 1 genome segment, linear ssRNA, 1 coat protein polypeptide
Cherry leafroll virus (CLRV)Isometric, about 30 nm, 2 genome
Chloris striate mosaic virus (CSMV)Geminate (arranged in pairs),
18 30 nm
Citrus leaf rugose virus (CiLRV)Isometric, 26-35 nm, 3 genome

Citrus tristeza virus (CTV)Flexuous rod, 600-2000 nm 12 nm,
Citrus variegation virus (CVV)Isometric, 26-35 nm, 3 genome
Clover yellow mosaic virus (ClYMV)Flexuous rod, 480-580 nm
Clover yellow vein virus (ClYVV)Flexuous rod, 680-900 nm 11 nm,
Clover yellows virus (CYV)Flexuous rod, 600-2000 nm 12 nm,
Cowpea aphid-borne mosaic virus (CABMV)Flexuous rod, 680-900
nm 11 nm, 1 genome segment, linear ssRNA, 1 coat protein
polypeptide
Cowpea mosaic virus (CPMV)Isometric, about 30 nm, 2 genome
Cucumber mosaic virus (CMV)Isometric, about 29 nm, 3 genome
Cymbidium mosaic virus (CybMV)Flexuous rod, 480-580 nm 13 nm,
Glycine mottle virus (GmoV)Isometric, about 34 nm, 1 genome
Grapevine fan leaf virus (GFLV)Isometric, about 30 nm, 2 genome
Lettuce mosaic virus (LMV)Flexuous rod, 680-900 nm 11 nm,
Lettuce necrotic yellows virus (LNYV)Bacilliform (enveloped), 360
52 nm, 1 genome segment, linear ssRNA, 5 coat protein polypeptides
Maize chlorotic dwarf virus (MCDV)Isometric, about 30 nm, 1linear
genome segment, ssRNA
Maize mosaic virus (MMV)Bacilliform, 240 48 nm, 1 genome
Maize streak virus (MSV)Geminate, 18 30 nm, 2 circular genome
segments, ssDNA, 1 coat protein polypeptide
Muskmelon vein necrosis virus (MkVNV)Flexuous rod, 600-700 nm
Narcissus yellow stripe virus (NYSV)Flexuous rod, 680-900 nm
Oat blue dwarf virus (OBDV)Polyhedral, 30 nm, 1 genome segment,
ssRNA
Oat mosaic virus (OMV)Flexuous rod, 680-900 nm 11 nm, 1 genome

Oat striate mosaic virus (OSMV)Bacilliform, 400 100 nm, 1 genome
Onion yellow dwarf virus (OYDV)Flexuous rod, 680-900 nm 11 nm,
Papaya mosaic virus (PapMV)Flexuous rod, 480-580 nm 13 nm,
Papaya ringspot virus (PRSV)Flexuous rod, 680-900 nm 11 nm,
Parsnip mosaic virus (ParMV)Flexuous rod, 680-900 nm 11 nm,
Pea early browning virus (PEBV)Rigid rod, 180-215 nm 22 nm and
46-114 nm 22 nm, 2 genome segments, 2 linear ssRNA, 1 coat protein polypeptide
Pea enation mosaic virus (PEMV)Polyhedral, about 30 nm, 2 genome
Pea leafroll virus (PeLRV)Polyhedral, 25-30 nm, 1 genome segment,
linear ssRNA, 1 coat protein polypeptide
Pea seedborne mosaic virus (PSMV)Flexuous rod, 680-900 nm 11 nm,
Pea streak virus (PeSV)Flexuous rod, 600-700 nm 13 nm, 1 genome
Peach rosette mosaic virus (PRMV)Isometric, about 30 nm, 2 genome
Peanut mottle virus (PeMoV)Flexuous rod, 680-900 nm 11 nm,
Peanut stunt virus (PSV)Isometric, about 29 nm, 3 genome segments,
Peanut yellow mottle virus (PYMV)isometric, about 29 nm, 1 genome
Pepino latent virus (PeLV)Flexuous rod, 600-700 nm 13 nm,
Pepper mottle virus (PepMoV)Flexuous rod, 680-900 nm 11 nm,
Pepper veinal mottle virus (PVMV)Flexuous rod, 680-900 nm 11 nm,
Plum pox virus (PPV)Flexuous rod, 680-900 nm 11 nm, 1 genome
Potato black ringspot virus (PBRV)Isometric, about 30 nm, 2 genome
Potato leafroll virus (PLRV)Polyhedral, 25-30 nm, 1 genome segment,

Potato virus A (PVA)Flexuous rod, 680-900 nm 11 nm, 1 genome
Potato virus M (PVM)Flexuous rod, 600-700 nm 13 nm, 1 genome
Potato virus S (PVS)Flexuous rod, 600-700 nm 13 nm, 1 genome
Potato virus X (PVX)Flexuous rod, 480-580 nm 13 nm, 1 genome
Potato virus Y (PVY)Flexuous rod, 680-900 nm 11 nm, 1 genome
Potato yellow dwarf virus (PYDV)Bacilliform, 380 75 nm, 1 genome
Prune dwarf virus (PDV)Isometric, 26-35 nm, 3 genome segments,
Prunus necrotic ringspot virus (PNRSV)Isometric, 26-35 nm,
Raspberry ringspot virus (RRV)Isometric, about 30 nm, 2 genome
Red clover necrotic mosaic virus (RCNMV)Isometric, about 34 nm,
Red clover vein mosaic virus (RCVMV)Flexuous rod, 600-700 nm
Rice black-streaked dwarf virus (RBSDV)Polyhedral, about 80 nm in
diameter, 10 genome segments, dsRNA, 5 coat protein polypeptides
Rice dwarf virus (RDV)Polyhedral, about 70 nm in diameter, 12 genome segments, dsRNA, 7 coat protein polypeptides
Rice necrosis mosaic virus (RNMV)Flexuous rod, 680-900 nm
Rice ragged stunt virus (RRSV)Polyhedral, about 50 nm in diameter,
10 genome segments, dsRNA, 5 coat protein polypeptides
Rice transitory yellowing virus (RTYV)Bullet shaped, 180-210 nm
9 nm, 1 genome segment, ssRNA, 4 or 5 coat protein polypeptides
Rice tungro bacilliform virus (RTBV)Bacilliform, 100-300 nm in
length and 30-35 nm in width, 1 genome segment, circular dsDNA,
1 coat protein polypeptide
Rice tungro spherical virus (RTSV)Polyhedral, about 30 nm in diameter, 1 genome segment, 3 coat protein polypeptides
Rice yellow mottle virus (RYMV)Polyhedral, about 30 nm, 1 genome
Shallot latent virus (SLV)Flexuous rod, 600-700 nm 13 nm,

Southern bean mosaic virus (SBMV)Polyhedral, about 30 nm,
Sowbane mosaic virus (SoMV)Polyhedral, about 30 nm, 1 genome
Soybean dwarf virus (SoyDV)Polyhedral, 25-30 nm, 1 genome
Soybean mosaic virus (SoyMV)Flexuous rod, 680-900 nm 11 nm,
Subterranean clover mottle virus (SCMoV)Polyhedral, about 30 nm,
Subterranean clover red leaf virus (SCRLV)Polyhedral, about 30 nm,
Sugarcane mosaic virus (SCMV)Flexuous rod, 680-900 nm 11 nm,
Sweet clover necrotic mosaic virus (SCNMV)Isometric, about 34 nm,
Tobacco etch virus (TEV)Flexuous rod, 680-900 nm 11 nm,
Tobacco mosaic virus (TMV)Rigid rod, about 300 nm 18 nm,
Tobacco necrosis virus (TNV)Polyhedral, about 30 nm, 1 genome
Tobacco necrotic dwarf virus (TNDV)Polyhedral, 25-30 nm, 1 genome
Tobacco rattle virus (TRV)Rigid rod, 180-215 nm 22 nm and
46-114 nm 22 nm, 2 genome segments, 2 linear ssRNA, 1 coat
protein polypeptide
Tobacco ringspot virus (TRSV)Isometric, about 30 nm, 2 genome
Tobacco streak virus (TSV)Isometric, 26-35 nm, 3 genome segments,
Tobacco vein mottling virus (TVMV)Flexuous rod, 680-900 nm 11 nm,
Tomato aspermy virus (TAV)Isometric, about 29 nm, 3 genome
Tomato black ring virus (TBRV)Isometric, about 30 nm, 2 genome
Tomato bushy stunt virus (TBSV)Isometric, about 34 nm, 1 genome
Tomato golden mosaic virus (ToGMV)Geminate, 18 30 nm, 2 circular genome segments, ssDNA, 1 coat protein polypeptide
Tomato mosaic virus (ToMV)Rigid rod, about 300 nm 18 nm,
Tomato ringspot virus (ToRSV)Isometric, about 30 nm, 2 genome
Tomato spotted wilt virus (TSWV)Spherical, about 85 nm, 4 genome
Tulip breaking virus (TBV)Flexuous rod, 680-900 nm 11 nm,
Turnip mosaic virus (TuMV)Flexuous rod, 680-900 nm 11 nm,
Turnip rosette virus (TRoV)Polyhedral, about 30 nm, 1 genome
Turnip yellow mosaic virus (TYMV)Isometric, about 29 nm, 1 genome
Watermelon mosaic virus (WMV)Flexuous rod, 680-900 nm 11 nm,
Wheat chlorotic streak virus (WCSV)Bacilliform, 355 55 nm,
Wheat yellow leaf virus (WYLV)Flexuous rod, 600-2000 nm 12 nm,
White clover mosaic virus (WClMV)Flexuous rod, 480-580 nm
MECHANISM OF PLANT VIRUS INFECTION
Viruses depend completely upon host cells for the supply of precursors,
energy, enzymes, and the structural machineryin fact for all the infrastructure except the coded message (virus nucleic acid)for their replication. Thus, viruses are parasites at the genetic level. The infection process
consists of viral attachment and ingress of the viral genome into the host cell.
Plant viruses possess no capacity of their own to push through the plant cell
wall barrier in order to enter the cell. They can come in contact with the cytoplasm or reach the interior of the plant cell only through wounds caused
mechanically or by vectors (Zaitlin and Hull, 1987). Wounding damages the
cuticle and cell wall and/or breaks the trichomes to expose specific attachment sites that differ from nonspecific attachment sites found on unabraded
cell walls (De Zoeten, 1995). The former exposes the cell membrane,
whereas the latter exposes the plasmodesmata. Plasmodesmata are abundant in the cell walls of hairs and also between hair cells and underlying epi-
dermal cells. In this way, virus particles become attached to the cell membrane. The virus may enter the protoplasts by pinocytosis or endocytosis,
the process by which substances enter the cell from the exterior and pass
into the cytoplasm. Attachment of virus particles to plasmalemma induces
the plasmalemmas invagination at the point of attachment. The neck of the
invagination closes, and the virus particle is imprisoned within an intracytoplasmic vesicle.
The virus may enter the cell by any one of the possible preexisting cell
mechanisms of nutrient uptake. Many invaginations of various sizes are normally present on the surface of plasmalemma of protoplasts, and the adsorption processes are most effective in these invaginations. The adsorbed virus
particles may enter the protoplast by closing the walls of invagination. The
resultant vesicle later may disintegrate to release virus particles into the cytoplasm. The adsorbed virus particles may also penetrate the protoplast by
the same type of electroosmotic forces responsible for transmembrane
transport of ions into the protoplast through plasmalemma.
Within a few seconds to minutes after entry of the virions (the complete
and infectious nucleoprotein particle of the virus) into the plant cell, uncoating of virus particles (i.e., removal of the envelope and coat protein and
release of the viral genome) occurs. TMV particles often uncoat within 15
to 30 min after inoculation. Uncoating of Tobacco necrosis virus particles
commences immediately after end-on attachment to the cell walls of plants.
It has been shown by several researchers that uncoating of virus particles occurs on the plant cell wall. Uncoating appears to be a nonspecific event.
Uncoating of the virus particles occurs not only on host plants but also with
equal efficiency on nonhosts. It has also been suggested that the uncoating
process may take place on the plasma membrane of pinocytic vesicles. The
virus particles uncoat and disappear from view from pinocytic vesicles
within a few minutes after their entry so that the viral genome, rather than
complete virus particles, enters the cytosol. According to some reports, the
uncoating process occurs intracellularly (i.e., in the cytoplasm). However,
this process has not been confirmed. It is most likely that this process occurs
outside of the cell (i.e., at an extracellular site).
The mechanism of uncoating is not yet fully understood (De Zoeten,
1995). It is suggested that more or less complete virus particles pass through
the plasmalemma. During their passage, these particles may encounter low
ionic (Ca2+) strength and Ca2+ ion gradients, the hydrophobic environment
of the plasmalemma, intracellular phospholipid membrane, or subcellular
low pH compartments. These conditions cause the virions to swell or lose
some protein subunits from the 5' end leader sequence so that partially
encapsidated virions enter the cytosol. There the ribosomes take over and
result in cotranslational disassembly. During translation, the ribosomes may
extract or release RNA from the spherical capsid without disrupting the
capsid completely. The RNA may escape through a hole formed in the
capsid by removal of some capsid protein units.
RNA-dependent RNA polymerase has been detected in many plants, and
it may be involved in viral replication in infected host cells. This enzyme is
activated by virus infection. However, host polymerase may not be the complete replicase needed for replication of a specific virus RNA (De Zoeten,
1995). A virus-coded polypeptide may associate with the host-specific enzyme to form a fully competent replicase (holoenzyme), which also possesses the needed specificity for viral RNA. Thus, a virus-encoded protein is
an integral component of the viral replicase. It is suggested that the complete virus-specific replicase is constituted by two units, a host-coded part
and a virus-coded part. The virus-coded part is a virally coded polypeptide
that functions in template selection. For example, Turnip yellow mosaic virus RNA replicase is made up of two major subunits: A 115-kilodalton
(kDa) molecular weight subunit encoded by the viral genome and a hostencoded 45 kDa unit that is different from the hosts RNA-dependent RNA
replicase.
Immediately after partial or complete uncoating, viral RNA directs the
synthesis of some early virus-specific proteins. One of these proteins is the
specific virus-encoded subunit of the polymerase complex, which is synthesized within 1 to 10 minutes after inoculation. Replication of viral RNA involves transcription of the virus (+) RNA into the complementary () chain
by the enzymes of the replicase system. The replication process starts from
the 3' end. Viral RNA acts as a template for the synthesis of complementary
strand. Thus viral RNA now exists as a double-stranded structure, the
replicative form (RF) of RNA. RF RNA is a full-length double-stranded
structure composed entirely of base pairs that is never infectious. The minus
strands of the initially dsRNA (RF RNA) are then released and act as templates for the synthesis of complementary plus strands of viral RNA (progeny RNA) by again generating dsRNA structures, the replicative intermediate (RI). Progeny plus strands generated may further enter into any one of
the three routes of the virus replication cycle: act as mRNA and code for
coat protein synthesis (the translation step), be converted to double-stranded
RF RNA to provide more templates for synthesis of viral plus RNA, or be
encapsidated to produce virions. The assembly of nucleic acid and coat protein into virions is almost a spontaneous process when viral nucleic acid and
specific proteins meet.
The replication process may vary slightly from virus to virus. For viruses
with () strand RNA (such as Rhabdovirus), special mRNA has first to be
transcribed. In the case of double-stranded RNA viruses such as Reovirus,
each of the ten to 12 segments may produce its own mRNA. DNA viruses
such as Caulimovirus use host polymerases for replication. All viruses have
to code for their specific replicase. Viral (+) RNA and complementary RNA
strands formed after transcription of the viral genome of some plant viruses
(Reovirus and Rhabdovirus) act as mRNA. This leads to the synthesis of virus-specific capsids and other proteins by translation of this mRNA. Two
types of ribosomes are present in plant cells: 80S cytoplasmic ribosomes
and 70S chloroplast and mitochondrial ribosomes. Plant viral mRNAs are
translated only on the 80S cytoplasmic ribosomes in several cases. Viral
proteins are formed on 80S ribosomes. Viral RNA may encode its specific
proteins in the midst of continuing synthesis of host proteins. Viruses have
only a few genes. Tobacco mosaic virus RNA carries information for at least
four proteins. The coat protein is encoded by the coat protein subgenomic
RNA. A second subgenomic RNA, I2 RNA, encodes the 30 kDa protein.
Another subgenomic RNA, I1 RNA, encodes a 50 kDa protein. These three
subgenomic RNAs occur on ribosomes and act as mRNAs (Mandahar,
1991).
Rice tungro bacilliform virus, a double-stranded DNA genome, has been
shown to have four open reading frames (ORFs). ORF I includes the gene
P24, which codes for a 24 kDa protein that may be associated with particle
assembly. The gene P12 in ORF II codes for a 12 kDa protein. The amino
acid sequence of the Rice tungro bacilliform virus ORF III product (P194)
has motifs suggestive of viral coat protein, aspartate protease, reverse transcriptase, and ribonuclease H. P194 is processed to give several products,
such as two coat proteins (37 kDa, 33 kDa), aspartate protease (21 kDa), reverse transcriptase (62 kDa), and ribonuclease H (55 kDa). ORF IV consists
of the gene P46 and may be involved in controlling expression of the RTBV
genome (Hull, 1996).
The plant virus genome encodes a wide range of proteins. All viruses
produce replicases and coat protein(s), and most probably encode one or
more proteins that potentiate virus movement from cell to cell and long distances in the plant. Some viruses, which are transmitted by insects, fungi, or
nematodes, often produce helper proteins that aid in the acquisition of the
virus by the vector. Some produce proteases that cleave the polyprotein.
Cauliflower mosaic virus encodes a multifunctional protein from gene VI, a
major component of the inclusion bodies that accumulates in infected cells.
Gene VI may play a role in virus assembly in the determination of the host
range of the virus and the severity of the symptoms (Zaitlin and Palukaitis,
2000).
Self-assembly of viral particles involves recognition of the specific
genomic RNA by specific capsid protein subunits and the assembly of viral
RNA with its specific proteins to form complete virus particles. Empty
capsids are also formed in some cases. Extensive virus synthesis occurs, and
complete virus particles gradually diffuse throughout the cytoplasm and ultimately lead to the formation of large virus aggregates or virus crystals.
Virus particles are synthesized in infected host cells and they spread from
cell to cell as well as long distances in host tissues. The molecular basis of
viral systemic movement has been studied in detail. Specific viral movement proteins are involved in the transfer of viral infectious transcripts
through plasmodesmata (Lucas, 1993). While competency for viruses to
replicate is produced by wounding, competency to transport is induced by
virus replication itself. Movement proteins are produced during virus replication. These proteins open the gates, i.e., the plasmodesmata, for transport
of the virus (Deom et al., 1992).
Plasmodesma is a membrane-lined pore (approximately 60 nm in diameter) through which passes a modified form of the endoplasmic reticulum,
termed the desmotubule or axial component. The axial component is continuous with the endoplasmic reticulum of the neighboring cells, but does not
have an associated lumen. It exists as a lipidic cylinder. In such a situation,
cytoplasmic continuity would occur through the cytoplasmic annulus, or
sleeve, that is located between the plasma membrane and the axial component. Void spaces within the cytoplasmic sleeve may have an effective diameter of 1.5-2.0 nm. Because the particles of most plant viruses are either
icosahedral with a diameter of 18-80 nm, helical or filamentous rods (rigid
or flexuous with diameters ranging from 10-25 nm and lengths of up to
2.5 m), their physical dimensions preclude their movement through unmodified plasmodesmata (Lucas, 1993). Numerous researchers have provided electron microscopic evidence that plant viruses have the capability to
cause dramatic changes in plasmodesmatal structure within systemically infected tissues (Esau, 1967; Roberts and Lucas, 1990; Lucas, 1993). Virions
have often been identified within the enlarged cytoplasmic sleeve of these
modified plasmodesmata. Their presence suggests that a viral encoded
product is involved in effecting these structural changes in the plasmodesmata. This product has been identified as a protein called movement
protein. A 30-kDa protein has been identified as the movement protein in
Tobacco mosaic virus. There are also reports that the virus coat protein is involved in long-distance transport (De Zoeten, 1995).
INCLUSION BODIES
Inclusion bodies are commonly found in virus-infected plants. They are
either amorphous, crystalline, or granular structures found in the cytoplasm
or in the nucleus. They differ in structure and composition, according to virus. Some contain high concentrations of virus particles embedded in amor-
phous material (e.g., Cauliflower mosaic virus). In cases of infection with

viruses such as Tobacco mosaic virus, the inclusion bodies consist of pure
virus in regular crystalline array. In cases of infection with Clover yellow
mosaic virus, the pure virus inclusion bodies are in banded array. Crystalline inclusion bodies are formed due to infection from some viruses belonging to the genus Potyvirus (potyviruses). These bodies consist of protein
formed as a result of virus infection. Crystalline inclusion bodies consisting
of small, noninfectious nucleoprotein particles are found in plants infected
with Red clover vein mosaic virus. Granular inclusions caused by Bean yellow mosaic virus and some other potyviruses contain peculiar pinwheels,
scrolls, and rolls, consisting of newly formed protein together with abnormal organelles. Thus, inclusion bodies consist of pure virus particles (viroplasm), virus-induced protein, or other material. These bodies can be detected by taking freehand sections, staining the infected leaves, and viewing
under a light microscope.
SATELLITE VIRUSES, SATELLITE RNA,
AND DEFECTIVE INTERFERING VIRUSES
Satellite viruses were first reported by Kassanis in 1962. Satellite viruses
have a coat but a small genome. They are dependent on other viruses to supply replicase and other enzymes necessary for replication. They act as parasites of plant-parasitic viruses. A satellite virus has been reported as being
associated with Tobacco necrosis virus (Kassanis, 1962). The viruses are
not serologically related. TNV is often found alone and can multiply indefinitely without causing the production of a satellite virus. However, the satellite virus is entirely dependent on TNV for its multiplication. Both viruses
occur in the roots of apparently normal plants and are transmitted among
roots by the fungus Olpidium brassicae.
The first satellite RNA described was the satellite RNA of Tobacco ringspot virus (Schneider, 1969). Satellite RNA has no coat protein. It completely depends upon those viruses for multiplication. Viruses that help
multiplication of satellite RNA are called helper viruses. Satellite RNA
aggravates or reduces the symptom development. This type of association
has also been reported in Cucumber mosaic virus (Tien and Wu, 1991).
Sometimes satellite viruses also may have satellite RNAs. The satellite of
Tobacco necrosis virus has been shown to contain a small satellite RNA that
is dependent on Tobacco necrosis virus for replication and on the satellite
virus for encapsulation.
Defective interfering viruses were first described in 1970 with an animal

virus (Zaitlin and Palukaitis, 2000). Several plant viruses in the family
Tombusviridae are now known to generate such RNAs during replication
(Rubio et al., 1999). In many cases, the viral symptoms are ameliorated
(Roux et al., 1991). However, in the case of Turnip crinkle virus, the defective interfering viruses are known to intensify viral symptoms.
CLASSIFICATION OF VIRUSES
Classification of plant viruses into class, order, family, genus, and species has not been achieved, mostly due to the lack of knowledge of genetic
relationships among the viruses. It is now known that viruses are biological
entities that possess genes, replicate, interact with hosts, and are exposed to
selection pressure, thus specializing and evolving. Recent molecular biological studies have facilitated grouping the viruses into genera. A few families have also been created to include related viruses. The formation of orders is very much in preliminary stage, and only one order has been created
thus far. The classification of plant viruses into orders, families, and genera
was formulated and approved by the International Committee on Taxonomy
of Viruses (ICTV) in 1995 and is being updated continually. The Latinized
binomial system is not being followed for virus classification, unlike the
classification of fungi and bacteria. At present, only groups of viruses have
been recognized and these groups are provisionally given the status of the
possible genera without establishing families. Each genus, regardless of
whether it is a member of a family, will have its own name. The virus species
is a class of viruses consisting of a replicating lineage and occupying a particular niche. This definition was approved by Executive Committee of
International Committee on Taxonomy of Viruses in 1991 at Atlanta. Species names are actually the vernacular name of the virus, indicating the common name of the plant and type of disease symptoms induced by the virus.
Virus names do not include the genus or group names. Van Regenmortel
(1999) provided guidelines for writing the names of species, genera, and
families of various viruses. The names of species, genera, and families
should be written in italics. The first word and proper noun in the name of
the species should be capitalized. Subsequent reference to the same virus
should be by the accepted acronym, which is not italicized. The ICTVs list
of approved virus genera and important virus species included in each genus
(Mayo and Horzinek, 1998; Van Regenmortel et al., 1999) is given here.
Many viruses remain unclassified, and their names are also given.
Genera of Plant Viruses and Names of Important Virus Species

in These Genera
Genus Alfamovirus: Alfalfa mosaic virus
Genus Alphacryptovirus : White clover cryptic virus 1, Beet 1 virus, Beet
2 virus
Genus Badnavirus: Banana streak virus, Cacao swollen shoot virus,
Piper yellow mottle virus, Rice tungro bacilliform virus, Sugarcane
bacilliform virus, Sweet potato leaf curl virus
Genus Betacryptovirus: White clover cryptic virus 2
Genus Bigeminivirus: Bean golden mosaic virus, Cassava African mosaic
virus, Cassava Indian mosaic virus, Cotton leaf crumple virus, Cotton
leaf curl virus, Dolichos yellow mosaic virus, Mungbean yellow mosaic
virus, Okra (bhendi) yellow vein mosaic virus, Pepper hausteco virus,
Potato yellow mosaic virus, Soybean crinkle leaf virus, Squash leaf
curl virus, Tobacco leaf curl virus, Tomato yellow leaf curl virus, Tomato golden mosaic virus, Tomato Indian leaf curl virus, Tomato mottle virus, Watermelon chlorotic stunt virus
Genus Bromovirus: Broad bean mottle virus, Cowpea chlorotic mottle
virus
Genus Bymovirus: Barley mild mosaic virus, Barley yellow mosaic virus,
Oat mosaic virus, Rice necrosis mosaic virus, Wheat yellow mosaic virus, Wheat spindle streak virus, Barley mild mottle virus
Genus Capillovirus: Apple stem grooving virus, Citrus tatter virus,
Cherry A virus
Genus Carlavirus: Blueberry scorch virus, Carnation latent virus, Chrysanthemum B virus, Hop mosaic virus, Cole latent virus, Cowpea mild
mottle virus, Poplar mosaic virus, Potato M virus, Potato S virus, Red
clover vein mosaic virus, Shallot latent virus, Passiflora latent virus,
Hop latent virus
Genus Carmovirus: Carnation mottle virus, Cowpeas mottle virus, Melon
necrotic spot virus, Pelargonium flower break virus, Turnip crinkle virus, Hibiscus chlorotic ringspot virus, Pelargonium line pattern virus,
Blackgram mottle virus, Narcissus tip necrosis virus
Genus Caulimovirus: Cassava vein mosaic virus, Cauliflower mosaic virus, Peanut chlorotic streak virus, Petunia vein-clearing virus, Strawberry vein-banding virus, Blueberry red ringspot virus, Carnation
etched ring virus, Figwort mosaic virus, Dahlia mosaic virus, Soybean
chlorotic mottle virus
Genus Closterovirus: Beet pseudo-yellows virus, Beet yellows virus, Citrus tristeza virus, Cucurbit yellow stunting disorder virus, Grapevine
corky bark-associated virus, Grapevine leafroll-associated virus,

Grapevine stem pitting-associated virus, Lettuce infectious yellows virus, Little cherry virus, Pineapple wilt-associated virus, Sweet potato
infectious chlorosis virus, Tomato infectious chlorosis virus, Beet yellow stunt virus, Sweet potato sunken vein virus
Genus Comovirus: Bean pod mottle virus, Bean rugose mosaic virus,
Bean severe mosaic virus, Broad bean stain virus, Cowpea mosaic virus, Cowpea severe mosaic virus, Potato Andean mottle virus, Red clover mottle virus, Squash mosaic virus
Genus Crinivirus: Sweet potato chlorotic stunt virus
Genus Cucumovirus: Cucumber mosaic virus, Peanut stunt virus, Tomato
aspermy virus
Genus Cytorhabdovirus: American wheat striate mosaic virus, Barley yellow striate mosaic virus, Festuca leaf streak virus, Lettuce necrotic yellows virus, Strawberry crinkle virus, Cereal northern mosaic virus
Genus Dianthovirus: Carnation ringspot virus, Red clover necrotic mosaic virus
Genus Enamovirus: Pea enation mosaic virus
Genus Fabavirus: Broad bean wilt virus
Genus Fijivirus: Maize rough dwarf virus, Oat sterile dwarf virus, Rice
black-streaked dwarf virus, Sugarcane Fiji disease virus
Genus Foveavirus: Apple stem pitting virus
Genus Furovirus: Beet necrotic yellow vein virus, Beet soilborne virus,
Broad bean necrosis virus, Oat golden stripe virus, Potato mop-top virus, Wheat soilborne mosaic virus, Peanut clump virus, Rice stripe necrosis virus
Genus Geminivirus: Taino tomato mottle virus, Tomato yellow vein streak
virus, Sinaloa tomato leaf curl virus, Tomato greenhouse whiteflyborne virus, Horse radish curly top virus, Tomato leaf curl virus
Genus Hordeivirus: Barley stripe mosaic virus
Genus Hybrigeminivirus: Beet curly top virus, Tomato pseudo-curly top
virus
Genus Idaeovirus: Raspberry bushy dwarf virus
Genus Ilarvirus: Apple mosaic virus, Asparagus 2 virus, Prune dwarf virus, Prunus necrotic ringspot virus, Spinach latent virus, Tobacco
streak virus, Citrus leaf rugose virus, Citrus variegation virus, Tomato
1 virus
Genus Ipomovirus: Sweet potato mild mottle virus
Genus Luteovirus: Barley yellow dwarf virus, Bean yellow dwarf virus,
Beet western yellows virus, Carrot red leaf virus, Cucurbit aphidborne
yellows virus, Groundnut rosette assistor virus, Potato leafroll virus,
Soybean dwarf virus, Subterranean clover red leaf virus, Beet mild yel-
lowing virus, Bean leaf roll virus, Pepper vein yellows virus, Soybean
Indonesian dwarf virus, Strawberry mild yellow edge virus
Genus Machlomovirus: Maize chlorotic mottle virus
Genus Macluravirus: Narcissus latent virus, Maclura mosaic virus
Genus Marafivirus: Maize rayado fino virus, Oat blue dwarf virus
Genus Monogeminivirus: Chickpea chlorotic dwarf virus, Maize streak virus, Wheat dwarf virus, Tobacco yellow dwarf virus, Sugarcane streak
virus
Genus Nanavirus: Banana bunchy top virus, Coconut foliar decay virus,
Faba bean necrotic yellows virus, Subterranean clover stunt virus
Genus Necrovirus: Tobacco necrosis virus
Genus Nepovirus: Arabis mosaic virus, Artichoke Italian latent virus,
Cherry leafroll virus, Cherry rosette virus, Grapevine chrome mosaic
virus, Grapevine fanleaf virus, Hibiscus latent ringspot virus, Raspberry ringspot virus, Satsuma dwarf virus, Strawberry latent ringspot
virus, Tobacco black ring virus, Tomato ringspot virus, Tomato black
ring virus, Cherry rasp leaf virus, Blueberry leaf mottle virus, Peach
rosette mosaic virus
Genus Nucleorhabdovirus: Maize mosaic virus, Raspberry vein chlorosis
virus, Sorghum stunt mosaic virus, Eggplant mottled dwarf virus
Genus Oleavirus: Olive latent 2 virus, Epirus cherry virus, Melon ourmia
virus
Genus Oryzavirus: Rice ragged stunt virus
Genus Phytoreovirus: Rice dwarf virus, Clover wound tumor virus
Genus Potexvirus: Bamboo mosaic virus, Cactus X virus, Cassava common mosaic virus, Clover yellow mosaic virus, Cymbidium mosaic virus, Foxtail mosaic virus, Hosta X virus, Lily X virus, Narcissus mosaic
virus, Papaya mosaic virus, Patchouli X virus, Potato aucuba mosaic
virus, Potato X virus, Strawberry mild yellow edge-associated virus,
Tulip X virus, Viola mottle virus, White clover mosaic virus
Genus Potyvirus: Abaca mosaic virus, Amazon lily mosaic virus, Artichoke latent virus, Asian prunus latent virus, Azuki bean mosaic virus,
Banana bract mosaic virus, Bean common mosaic virus, Bean yellow
mosaic virus, Beet mosaic virus, Blackeye cowpea mosaic virus, Cardamom mosaic virus, Carnation vein mottle virus, Cassava brown
streak virus, Celery mosaic virus, Chilli veinal mottle virus, Clover yellow vein virus, Cowpea aphidborne mosaic virus, Dasheen mosaic virus, Datura Colombian virus, Garlic yellow streak virus, Johnsongrass
mosaic virus, Kalanchoe mosaic virus, Leek yellow stripe virus, Lettuce mosaic virus, Maize dwarf mosaic virus, Narcissus yellow stripe
virus, Onion yellow dwarf virus, Palm mosaic virus, Papaya leaf-distortion mosaic virus, Papaya ringspot virus, Passion fruit woodiness
virus, Pea seedborne mosaic virus, Peanut chlorotic blotch virus, Peanut mottle virus, Peanut stripe virus, Pepper severe mosaic virus, Pepper mottle virus, Pepper veinal mottle virus, Plum pox virus, Potato A
virus, Potato V virus, Potato Y virus, Radish vein clearing virus, Sorghum mosaic virus, Soybean mosaic virus, Sugarcane mosaic virus,
Sweet potato feathery mottle virus, Sweet potato latent virus, Sweet potato mild speckling virus, Tamarillo mosaic virus, Taro feathery mottle
virus, Tobacco etch virus, Tobacco vein mottling virus, Tulip breaking
virus, Turnip mosaic virus, Vanilla necrosis virus, Watermelon Moroccan mosaic virus, Watermelon mosaic 1 virus, Watermelon mosaic 2 virus, Welsh onion yellow stripe virus, Yam mosaic virus, Zucchini yellow fleck virus, Zucchini yellow mosaic virus
Genus Rhabdovirus: Beet leaf curl virus, Citrus leprosis virus, Orchid
fleck virus
Genus Rymovirus: Agropyron mosaic virus, Brome streak mosaic virus,
Garlic miteborne mosaic virus, Hordeum mosaic virus, Ryegrass mosaic virus, Wheat leaf streak virus, Wheat streak mosaic virus
Genus Satellivirus: Panicum mosaic satellivirus, Tobacco mosaic
satellivirus, Tobacco necrosis satellivirus
Genus Sequivirus: Dandelion yellow mosaic virus, Parsnip yellow fleck
virus
Genus Sobemovirus: Bean southern mosaic virus, Blueberry shoestring
virus, Clover mottle virus, Lucerne transient streak virus, Olive latent
1 virus, Panicum mosaic virus, Rice yellow mottle virus, Sowbane mosaic virus, Subterranean clover mottle virus
Genus Tenuivirus: Maize stripe virus, Rice hoja blanca virus, Rice grassy
stunt virus, Rice stripe virus, Wheat Iranian stripe virus, Echinochloa
hoja blanca virus, Maize yellow stripe virus
Genus Tobamovirus: Cucumber green mottle mosaic virus, Paprika mild
mottle virus, Pepper mild mottle virus, Sunnhemp mosaic virus, Tomato
mosaic virus, Tobacco mosaic virus, Tobacco mild green mosaic virus,
Turnip vein-clearing virus
Genus Tobravirus: Pea early browning virus, Tobacco rattle virus, Tobacco ringspot virus
Genus Tombusvirus: Tomato bushy stunt virus, Carnation Italian ringspot
virus, Oat chlorotic stunt virus, Artichoke mottled crinkle virus, Cymbidium ringspot virus, Petunia asteroid mosaic virus, Cucumber necrosis
virus
Genus Tospovirus: Groundnut ringspot virus, Peanut bud necrosis virus,
Peanut yellow spot virus, Tomato spotted wilt virus, Tomato chlorotic
spot virus, Impatiens necrotic spot virus, Iris yellow spot virus
Genus Trichovirus: Apple chlorotic leaf spot virus, Cherry mottle leaf virus, Grapevine berry inner necrosis virus
Genus Tymovirus: Turnip yellow mosaic virus, Melon rugose mosaic virus, Physalis mottle virus, Eggplant mosaic virus, Belladonna mottle
virus, Calopogonium yellow vein virus, Okra mosaic virus, Poinsettia
mosaic virus, Potato Andean latent virus, Sesbania mosaic virus
Genus Umbravirus: Carrot mottle virus, Groundnut rosette virus, Sunflower yellow blotch virus
Genus Varicosavirus: Lettuce big-vein virus
Genus Vitivirus: Grapevine A virus, Grapevine B virus
Genus Waikavirus: Rice tungro spherical virus, Maize chlorotic dwarf
virus
Unclassified viruses: Banana dieback virus, Banana mosaic virus, Bean
line pattern mosaic virus, Beet soilborne mosaic virus, Beet virus Q,
Black currant reversion associated virus, Black raspberry necrosis virus, Broad bean bushy dwarf virus, Citrus infectious variegation virus,
Citrus mosaic virus, Citrus ringspot virus, Citrus psorosis virus, Citrus
vein enation virus, East African cassava mosaic virus, Garlic A virus,
Garlic latent virus, Garlic X virus, Garlic yellow stripe virus, Grapevine fleck virus, Groundnut bud necrosis virus, Havana tomato virus,
Ipomoea crinkle leaf curl virus, Lettuce chlorosis virus, Papaya leaf
curl virus, Papaya lethal yellowing virus, Peach mosaic virus, Peanut
chlorotic fan-spot virus, Pepper vein banding virus, Pigeonpea sterility
mosaic virus, Raspberry leaf spot virus, Raspberry leaf mottle virus,
Rice yellow stunt virus, Sour cherry green ring mottle virus, Squash
yellow leaf curl virus, Sugarcane mild mosaic virus, Sugarcane yellow
leaf virus, Sunflower mosaic virus, Tomato chlorosis virus, Urd bean
leaf crinkle virus, Yam mild mosaic virus
Families of Plant Viruses
A few genera have been grouped into families. The recognized families
containing plant viruses are as follows:
1. Family: Bromoviridae
Genera: Alfamovirus, Bromovirus, Cucumovirus, Ilarvirus, Oleavirus
2. Family: Bunyaviridae
Genera: Tospovirus (Other genera such as Bunyavirus, Nairovirus,
Hantavirus, and Phlebovirus are not plant viruses)
3. Family: Caulimoviridae
Genera: Badnavirus, Caulimovirus
4. Family: Comoviridae
Genera: Comovirus, Fabavirus, Nepovirus
5. Family: Geminiviridae
Genera: Mastrevirus (Subgroup I Geminivirus)
Curtovirus (Subgroup II Geminivirus)
Begomovirus (Subgroup III Geminivirus)
6. Family: Partitiviridae
Genera: Alphacryptovirus, Betacryptovirus
7. Family: Potyviride
Genera: Bymovirus, Potyvirus, Rymovirus
8. Family: Reoviridae
Genera: Fijivirus, Oryzavirus, Phytoreovirus
9. Family: Rhabdoviridae
Genera: Cytorhabdovirus, Nucleorhabdovirus
10. Family: Sequiviridae
Genera: Sequivirus, Waikavirus
11. Family: Tombusviridae
Genera: Carmovirus, Tombusvirus
An Order That Contains Plant Viruses
Order: Mononegavirales
Family: Rhabdoviridae
Genera: Cytorhabdovirus, Nucleorhabdovirus
SYSTEMATIC POSITION OF IMPORTANT VIRAL PATHOGENS

Abaca mosaic virusgenus Potyvirus, family Potyviridae
Agropyron mosaic virusgenus Rymovirus, family Potyviridae
Alfalfa cryptic virusunclassified
Alfalfa mosaic virusgenus Alfamovirus, family Bromoviridae
Amazon lily mosaic virusgenus Potyvirus, family Potyviridae
American wheat striate mosaic virusgenus Cytorhabdovirus, family
Rhabdoviridae, order Mononegavirales
Apple chlorotic leaf spot virusgenus Trichovirus
Apple mosaic virusgenus Ilarvirus, family Bromoviridae
Apple stem grooving virusgenus Capillovirus
Apple stem pitting virusgenus Foveavirus
Arabis mosaic virusgenus Nepovirus, family Comoviridae
Artichoke Italian latent virusgenus Nepovirus, family Comoviridae
Artichoke latent virusgenus Potyvirus, family Potyviridae

Artichoke mottled crinkle virusgenus Tombusvirus
Asian prunus latent virusgenus Potyvirus, family Potyviridae
Asparagus 2 virusgenus Ilarvirus, family Bromoviridae
Azuki bean mosaic virusgenus Potyvirus, family Potyviridae
Bamboo mosaic virusgenus Potexvirus
Banana bract mosaic virusgenus Potyvirus, family Potyviridae
Banana bunchy top virusgenus Nanavirus
Banana dieback virusunclassified
Banana mosaic virusunclassified
Banana streak virusgenus Badnavirus, family Caulimoviridae
Barley mild mosaic virusgenus Bymovirus, family Potyviridae
Barley mild mottle virusgenus Bymovirus, family Potyviridae
Barley stripe mosaic virusgenus Hordeivirus
Barley yellow dwarf virusgenus Luteovirus
Barley yellow mosaic virusgenus Bymovirus, family Potyviridae
Barley yellow striate mosaic virusgenus Cytorhabdovirus, family
Bean common mosaic virusgenus Potyvirus, family Potyviridae
Bean curly dwarf mosaic virusgenus Comovirus, family Comoviridae
Bean golden mosaic virusgenus Bigeminivirus
Bean leafroll virusgenus Luteovirus
Bean line pattern mosaic virusunclassified
Bean mild mosaic virusunclassified
Bean pod mottle virusgenus Comovirus, family Comoviridae
Bean rugose mosaic virusgenus Comovirus, family Comoviridae
Bean severe mosaic virusgenus Comovirus, family Comoviridae
Bean southern mosaic virusgenus Sobemovirus
Bean yellow dwarf virusgenus Luteovirus
Bean yellow mosaic virusgenus Potyvirus, family Potyviridae
Beet 1 virusgenus Alphacryptovirus, family Partitiviridae
Beet 2 virusgenus Alphacryptovirus, family Partitiviridae
Beet curly top virusgenus Hybrigeminivirus
Beet leaf curl virusgenus Rhabdovirus
Beet mild yellowing virusgenus Luteovirus
Beet mosaic virusgenus Potyvirus, family Potyviridae
Beet necrotic yellow vein virusgenus Furovirus
Beet pseudo-yellows virusgenus Closterovirus
Beet soilborne mosaic virusunclassified
Beet soilborne virusgenus Furovirus
Beet virus Qunclassified
Beet western yellows virusgenus Luteovirus
Beet yellow stunt virusgenus Closterovirus

Beet yellows virusgenus Closterovirus
Belladonna mottle virusgenus Tymovirus
Black currant reversion associated virusunclassified
Black raspberry necrosis virusunclassified
Blackeye cowpea mosaic virusgenus Potyvirus, family Potyviridae
Blackgram mottle virusgenus Carmovirus, family Tombusviridae
Blueberry leaf mottle virusgenus Nepovirus, family Comoviridae
Blueberry red ringspot virusgenus Caulimovirus, family
Caulimoviridae
Blueberry scorch virusgenus Carlavirus
Blueberry shoestring virusgenus Sobemovirus
Broad bean bushy dwarf virusunclassified
Broad bean mottle virusgenus Bromovirus, family Bromoviridae
Broad bean necrosis virusgenus Furovirus
Broad bean stain virusgenus Comovirus, family Comoviridae
Broad bean wilt virusgenus Fabavirus, family Comoviridae
Brome streak mosaic virusgenus Rymovirus, family Potyviridae
Cacao swollen shoot virusgenus Badnavirus, family Caulimoviridae
Cactus X virusgenus Potexvirus
Calopogonium yellow vein virusgenus Tymovirus
Cardamom mosaic virusgenus Potyvirus, family Potyviridae
Carnation etched ring virusgenus Caulimovirus, family Caulimoviridae
Carnation Italian ringspot virusgenus Tombusvirus
Carnation latent virusgenus Carlavirus
Carnation mottle virusgenus Carmovirus, family Tombusviridae
Carnation necrotic fleck virusgenus Closterovirus
Carnation ringspot virusgenus Dianthovirus
Carnation vein mottle virusgenus Potyvirus, family Potyviridae
Carrot latent virusgenus Nucleorhabdovirus
Carrot mottle virusgenus Umbravirus
Carrot red leaf virusgenus Luteovirus
Cassava African mosaic virusgenus Bigeminivirus
Cassava brown streak virusgenus Potyvirus, family Potyviridae
Cassava common mosaic virusgenus Potexvirus
Cassava Indian mosaic virusgenus Bigeminivirus
Cassava vein mosaic virusgenus Caulimovirus, family Caulimoviridae
Cauliflower mosaic virusgenus Caulimovirus, family Caulimoviridae
Celery mosaic virusgenus Potyvirus, family Potyviridae
Cereal northern mosaic virusgenus Cytorhabdovirus, family
Cherry A virusgenus Capillovirus
Cherry leafroll virusgenus Nepovirus, family Comoviridae

Cherry mottle leaf virusgenus Trichovirus
Cherry rasp leaf virusgenus Nepovirus, family Comoviridae
Cherry rosette virusgenus Nepovirus, family Comoviridae
Chickpea chlorotic dwarf virusgenus Monogeminivirus
Chickpea virusgenus Luteovirus
Chilli veinal mottle virusgenus Potyvirus, family Potyviridae
Chrysanthemum B virusgenus Carlavirus
Citrus infectious variegation virusunclassified
Citrus leaf rugose virusgenus Ilarvirus, family Bromoviridae
Citrus leprosis virusgenus Rhabdovirus
Citrus mosaic virusunclassified
Citrus psorosis virusunclassified
Citrus ringspot virusunclassified
Citrus tatter virusgenus Capillovirus
Citrus tristeza virusgenus Closterovirus
Citrus variegation virusgenus Ilarvirus, family Bromoviridae
Citrus vein enation virusunclassified
Clover mottle virusgenus Sobemovirus
Clover wound tumor virusgenus Phytoreovirus, family Reoviridae
Clover yellow mosaic virusgenus Potexvirus
Clover yellow vein virusgenus Potyvirus, family Potyviridae
Coconut foliar decay virusgenus Nanavirus
Cole latent virusgenus Carlavirus
Cotton leaf crumple virusgenus Bigeminivirus
Cotton leaf curl virusgenus Bigeminivirus
Cowpea aphidborne mosaic virusgenus Potyvirus, family Potyviridae
Cowpea chlorotic mottle virusgenus Bromovirus, family Bromoviridae
Cowpea mild mottle virusgenus Carlavirus
Cowpea mosaic virusgenus Comovirus, family Comoviridae
Cowpea severe mosaic virusgenus Comovirus, family Comoviridae
Cowpeas mottle virusgenus Carmovirus, family Tombusviridae
Cucumber green mottle mosaic virusgenus Tobamovirus
Cucumber mosaic virusgenus Cucumovirus, family Bromoviridae
Cucumber necrosis virusgenus Tombusvirus
Cucurbit aphidborne yellows virusgenus Luteovirus
Cucurbit yellow stunting disorder virusgenus Closterovirus
Cymbidium mosaic virusgenus Potexvirus
Cymbidium ringspot virusgenus Tombusvirus
Dahlia mosaic virusgenus Caulimovirus, family Caulimoviridae
Dandelion yellow mosaic virusgenus Sequivirus, family Sequiviridae
Dasheen mosaic virusgenus Potyvirus, family Potyviridae
Datura Colombian virusgenus Potyvirus, family Potyviridae

Dolichos yellow mosaic virusgenus Bigeminivirus
East African cassava mosaic virusunclassified
Echinochloa hoja blanca virusgenus Tenuivirus
Eggplant mosaic virusgenus Tymovirus
Eggplant mottled dwarf virusgenus Nucleorhabdovirus, family
Epirus cherry virusgenus Oleavirus, family Bromoviridae
Faba bean necrotic yellows virusgenus Nanavirus
Festuca leaf streak virusgenus Cytorhabdovirus, family Rhabdoviridae,
order Mononegavirales
Figwort mosaic virusgenus Caulimovirus, family Caulimoviridae
Foxtail mosaic virusgenus Potexvirus
Garlic A virusunclassified
Garlic latent virusunclassified
Garlic miteborne mosaic virusgenus Rymovirus, family Potyviridae
Garlic X virusunclassified
Garlic yellow streak virusgenus Potyvirus, family Potyviridae
Garlic yellow stripe virusunclassified
Grapevine A virusgenus Vitivirus
Grapevine B virusgenus Vitivirus
Grapevine berry inner necrosis virusgenus Trichovirus
Grapevine chrome mosaic virusgenus Nepovirus, family Comoviridae
Grapevine corky bark-associated virusgenus Closterovirus
Grapevine fanleaf virusgenus Nepovirus, family Comoviridae
Grapevine fleck virusunclassified
Grapevine leafroll-associated virusgenus Closterovirus
Grapevine stem pitting-associated virusgenus Closterovirus
Groundnut bud necrosis virusunclassified
Groundnut ringspot virusgenus Tospovirus
Groundnut rosette assistor virusgenus Luteovirus
Groundnut rosette virusgenus Umbravirus
Havana tomato virusunclassified
Hibiscus chlorotic ringspot virusgenus Carmovirus, family
Tombusviridae
Hibiscus latent ringspot virusgenus Nepovirus, family Comoviridae
Hop latent virusgenus Carlavirus
Hop mosaic virusgenus Carlavirus
Hordeum mosaic virusgenus Rymovirus, family Potyviridae
Horse radish curly top virusgenus Geminivirus, family Geminiviridae
Hosta X virusgenus Potexvirus
Impatiens necrotic spot virusgenus Tospovirus, family Bunyaviridae
Ipomoea crinkle leaf curl virusunclassified

Iris yellow spot virusgenus Tospovirus
Johnsongrass mosaic virusgenus Potyvirus, family Potyviridae
Kalanchoe mosaic virusgenus Potyvirus, family Potyviridae
Leek yellow stripe virusgenus Potyvirus, family Potyviridae
Lettuce big-vein virusgenus Varicosavirus
Lettuce chlorosis virusunclassified
Lettuce infectious yellows virusgenus Closterovirus
Lettuce mosaic virusgenus Potyvirus, family Potyviridae
Lettuce necrotic yellows virusgenus Cytorhabdovirus, family
Lettuce speckles mottle virusgenus Umbravirus
Lily X virusgenus Potexvirus
Little cherry virusgenus Closterovirus
Lucerne transient streak virusgenus Sobemovirus
Maclura mosaic virusgenus Macluravirus
Maize chlorotic dwarf virusgenus Waikavirus
Maize chlorotic mottle virusgenus Machlomovirus
Maize dwarf mosaic virusgenus Potyvirus, family Potyviridae
Maize mosaic virusgenus Nucleorhabdovirus, family Rhabdoviridae,
Maize rayado fino virusgenus Marafivirus
Maize rough dwarf virusgenus Fijivirus, family Reoviridae
Maize streak virusgenus Monogeminivirus
Maize stripe virusgenus Tenuivirus
Maize yellow stripe virusgenus Tenuivirus
Melon necrotic spot virusgenus Carmovirus, family Tombusviridae
Melon ourmia virusgenus Oleavirus, family Bromoviridae
Melon rugose mosaic virusgenus Tymovirus
Mungbean yellow mosaic virusgenus Bigeminivirus
Narcissus latent virusgenus Macluravirus
Narcissus mosaic virusgenus Potexvirus
Narcissus tip necrosis virusgenus Carmovirus, family Tombusviridae
Narcissus yellow stripe virusgenus Potyvirus, family Potyviridae
Oat blue dwarf virusgenus Marafivirus
Oat chlorotic stunt virusgenus Tombusvirus
Oat golden stripe virusgenus Furovirus
Oat mosaic virusgenus Bymovirus, family Potyviridae
Oat sterile dwarf virusgenus Fijivirus, family Reoviridae
Okra (bhendi) yellow vein mosaic virusgenus Bigeminivirus
Okra mosaic virusgenus Tymovirus
Olive latent 1 virusgenus Sobemovirus
Olive latent 2 virusgenus Oleavirus, family Bromoviridae

Onion yellow dwarf virusgenus Potyvirus, family Potyviridae
Orchid fleck virusgenus Rhabdovirus
Palm mosaic virusgenus Potyvirus, family Potyviridae
Panicum mosaic satellivirusgenus Satellivirus
Panicum mosaic virusgenus Sobemovirus
Papaya apical necrosis virusgenus Rhabdovirus
Papaya droopy necrosis virusgenus Rhabdovirus
Papaya leaf curl virusunclassified
Papaya leaf-distortion mosaic virusgenus Potyvirus, family Potyviridae
Papaya lethal yellowing virusunclassified
Papaya mosaic virusgenus Potexvirus
Papaya ringspot virusgenus Potyvirus, family Potyviridae
Paprika mild mottle virusgenus Tobamovirus
Parsnip yellow fleck virusgenus Sequivirus, family Sequiviridae
Passiflora latent virusgenus Carlavirus
Passion fruit woodiness virusgenus Potyvirus, family Potyviridae
Patchouli X virusgenus Potexvirus
Pea early browning virusgenus Tobravirus
Pea enation mosaic virusgenus Enamovirus
Pea seedborne mosaic virusgenus Potyvirus, family Potyviridae
Pea streak virusgenus Carlavirus
Peach mosaic virusunclassified
Peach rosette mosaic virusgenus Nepovirus, family Comoviridae
Peanut bud necrosis virusgenus Tospovirus, family Bunyaviridae
Peanut chlorotic blotch virusgenus Potyvirus, family Potyviridae
Peanut chlorotic fan-spot virusunclassified
Peanut chlorotic streak virusgenus Caulimovirus, family
Caulimoviridae
Peanut clump virusgenus Furovirus
Peanut mottle virusgenus Potyvirus, family Potyviridae
Peanut stripe virusgenus Potyvirus, family Potyviridae
Peanut stunt virusgenus Cucumovirus, family Bromoviridae
Peanut yellow spot virusgenus Tospovirus, family Bunyaviridae
Pelargonium flower break virusgenus Carmovirus, family
Tombusviridae
Pelargonium line pattern virusgenus Carmovirus, family Tombusviridae
Pepper hausteco virusgenus Bigeminivirus
Pepper mild mottle virusgenus Tobamovirus
Pepper mottle virusgenus Potyvirus, family Potyviridae
Pepper severe mosaic virusgenus Potyvirus, family Potyviridae
Pepper vein banding virusunclassified
Pepper vein yellows virusgenus Luteovirus

Pepper veinal mottle virusgenus Potyvirus, family Potyviridae
Petunia asteroid mosaic virusgenus Tombusvirus
Petunia vein-clearing virusgenus Caulimovirus, family Caulimoviridae
Physalis mottle virusgenus Tymovirus
Pigeonpea sterility mosaic virusunclassified
Pineapple wilt-associated virusgenus Closterovirus
Piper yellow mottle virusgenus Badnavirus, family Caulimoviridae
Plum pox virusgenus Potyvirus, family Potyviridae
Poinsettia mosaic virusgenus Tymovirus
Poplar mosaic virusgenus Carlavirus
Potato A virusgenus Potyvirus, family Potyviridae
Potato Andean latent virusgenus Tymovirus
Potato Andean mottle virusgenus Comovirus, family Comoviridae
Potato aucuba mosaic virusgenus Potexvirus
Potato leafroll virusgenus Luteovirus
Potato M virusgenus Carlavirus
Potato mop-top virusgenus Furovirus
Potato S virusgenus Carlavirus
Potato T virusgenus Trichovirus
Potato V virusgenus Potyvirus, family Potyviridae
Potato X virusgenus Potexvirus
Potato Y virusgenus Potyvirus, family Potyviridae
Potato yellow mosaic virusgenus Bigeminivirus
Prune dwarf virusgenus Ilarvirus, family Bromoviridae
Prunus necrotic ringspot virusgenus Ilarvirus, family Bromoviridae
Radish vein clearing virusgenus Potyvirus, family Potyviridae
Raspberry bushy dwarf virusgenus Idaeovirus
Raspberry leaf mottle virusunclassified
Raspberry leaf spot virusunclassified
Raspberry ringspot virusgenus Nepovirus, family Comoviridae
Raspberry vein chlorosis virusgenus Nucleorhabdovirus, family
Red clover mottle virusgenus Comovirus, family Comoviridae
Red clover necrotic mosaic virusgenus Dianthovirus
Red clover vein mosaic virusgenus Carlavirus
Rice black-streaked dwarf virusgenus Fijivirus, family Reoviridae
Rice dwarf virusgenus Phytoreovirus, family Reoviridae
Rice gall dwarf virusgenus Phytoreovirus, family Reoviridae
Rice grassy stunt virusgenus Tenuivirus
Rice hoja blanca virusgenus Tenuivirus
Rice necrosis mosaic virusgenus Bymovirus, family Potyviridae
Rice ragged stunt virusgenus Oryzavirus, family Reoviridae

Rice stripe necrosis virusgenus Furovirus
Rice stripe virusgenus Tenuivirus
Rice tungro bacilliform virusgenus Badnavirus, family Caulimoviridae
Rice tungro spherical virusgenus Waikavirus, family Sequiviridae
Rice yellow mottle virusgenus Sobemovirus
Rice yellow stunt virusunclassified
Ryegrass mosaic virusgenus Rymovirus, family Potyviridae
Satsuma dwarf virusgenus Nepovirus, family Comoviridae
Sesbania mosaic virusgenus Tymovirus
Shallot latent virusgenus Carlavirus
Sinaloa tomato leaf curl virusgenus Geminivirus, family Geminiviridae
Sorghum mosaic virusgenus Potyvirus, family Potyviridae
Sorghum stunt mosaic virusgenus Nucleorhabdovirus, family
Sour cherry green ring mottle virusunclassified
Sowbane mosaic virusgenus Sobemovirus
Soybean chlorotic mottle virusgenus Caulimovirus, family
Caulimoviridae
Soybean crinkle leaf virusgenus Bigeminivirus
Soybean dwarf virusgenus Luteovirus
Soybean Indonesian dwarf virusgenus Luteovirus
Soybean mosaic virusgenus Potyvirus, family Potyviridae
Soybean virusgenus Rhabdovirus
Spinach latent virusgenus Ilarvirus, family Bromoviridae
Squash leaf curl virusgenus Bigeminivirus
Squash mosaic virusgenus Comovirus, family Comoviridae
Squash yellow leaf curl virusunclassified
Strawberry crinkle virusgenus Cytorhabdovirus, family Rhabdoviridae,
Strawberry latent ringspot virusgenus Nepovirus, family Comoviridae
Strawberry mild yellow edge virusgenus Luteovirus
Strawberry mild yellow edge-associated virusgenus Potexvirus
Strawberry vein banding virusgenus Caulimovirus, family
Caulimoviridae
Subterranean clover mottle virusgenus Sobemovirus
Subterranean clover red leaf virusgenus Luteovirus
Subterranean clover stunt virusgenus Nanavirus
Sugarcane bacilliform virusgenus Badnavirus, family Caulimoviridae
Sugarcane Fiji disease virusgenus Fijivirus, family Reoviridae
Sugarcane mild mosaic virusunclassified
Sugarcane mosaic virusgenus Potyvirus, family Potyviridae
Sugarcane streak virusgenus Monogeminivirus

Sugarcane yellow leaf virusunclassified
Sunflower mosaic virusunclassified
Sunflower yellow blotch virusgenus Umbravirus
Sunnhemp mosaic virusgenus Tobamovirus
Sweet potato chlorotic stunt virusgenus Crinivirus
Sweet potato feathery mottle virusgenus Potyvirus, family Potyviridae
Sweet potato infectious chlorosis virusgenus Closterovirus
Sweet potato latent virusgenus Potyvirus, family Potyviridae
Sweet potato leaf curl virusgenus Badnavirus, family Caulimoviridae
Sweet potato mild mottle virusgenus Ipomovirus
Sweet potato mild speckling virusgenus Potyvirus, family Potyviridae
Sweet potato sunken vein virusgenus Closterovirus
Taino tomato mottle virusgenus Geminivirus, family Geminiviridae
Tamarillo mosaic virusgenus Potyvirus, family Potyviridae
Taro feathery mottle virusgenus Potyvirus, family Potyviridae
Tobacco black ring virusgenus Nepovirus, family Comoviridae
Tobacco etch virusgenus Potyvirus, family Potyviridae
Tobacco leaf curl virusgenus Bigeminivirus
Tobacco mild green mosaic virusgenus Tobamovirus
Tobacco mosaic satellivirusgenus Satellivirus
Tobacco mosaic virusgenus Tobamovirus
Tobacco necrosis satellivirusgenus Satellivirus
Tobacco necrosis virusgenus Necrovirus
Tobacco rattle virusgenus Tobravirus
Tobacco ringspot virusgenus Tobravirus
Tobacco streak virusgenus Ilarvirus, family Bromoviridae
Tobacco vein mottling virusgenus Potyvirus, family Potyviridae
Tobacco yellow dwarf virusgenus Monogeminivirus
Tomato 1 virusgenus Ilarvirus, family Bromoviridae
Tomato aspermy virusgenus Cucumovirus, family Bromoviridae
Tomato black ring virusgenus Nepovirus, family Comoviridae
Tomato bushy stunt virusgenus Tombusvirus
Tomato chlorosis virusunclassified
Tomato chlorotic spot virusgenus Tospovirus
Tomato golden mosaic virusgenus Bigeminivirus
Tomato greenhouse whitefly-borne virusgenus Geminivirus, family
Geminiviridae
Tomato Indian leaf curl virusgenus Bigeminivirus
Tomato infectious chlorosis virusgenus Closterovirus
Tomato leaf curl virusgenus Geminivirus, family Geminiviridae
Tomato mosaic virusgenus Tobamovirus
Tomato mottle virusgenus Bigeminivirus

Tomato pseudo-curly top virusgenus Hybrigeminivirus
Tomato ringspot virusgenus Nepovirus, family Comoviridae
Tomato spotted wilt virusgenus Tospovirus, family Bunyaviridae
Tomato yellow leaf curl virusgenus Bigeminivirus
Tomato yellow vein streak virusgenus Geminivirus, family
Geminiviridae
Tulip breaking virusgenus Potyvirus, family Potyviridae
Tulip X virusgenus Potexvirus
Turnip crinkle virusgenus Carmovirus, family Tombusviridae
Turnip mosaic virusgenus Potyvirus, family Potyviridae
Turnip vein-clearing virusgenus Tobamovirus
Turnip yellow mosaic virusgenus Tymovirus
Urd bean leaf crinkle virusunclassified
Vanilla necrosis virusgenus Potyvirus, family Potyviridae
Viola mottle virusgenus Potexvirus
Watermelon chlorotic stunt virusgenus Bigeminivirus
Watermelon Moroccan mosaic virusgenus Potyvirus, family Potyviridae
Watermelon mosaic 1 virusgenus Potyvirus, family Potyviridae
Watermelon mosaic 2 virusgenus Potyvirus, family Potyviridae
Watermelon silvery mottle virusgenus Tospovirus, family Bunyaviridae
Welsh onion yellow stripe virusgenus Potyvirus, family Potyviridae
Wheat dwarf virusgenus Monogeminivirus
Wheat Iranian stripe virusgenus Tenuivirus
Wheat leaf streak virusgenus Rymovirus, family Potyviridae
Wheat soilborne mosaic virusgenus Furovirus
Wheat spindle streak virusgenus Bymovirus, family Potyviridae
Wheat streak mosaic virusgenus Rymovirus, family Potyviridae
Wheat yellow mosaic virusgenus Bymovirus, family Potyviridae
White clover cryptic virus 1genus Alphacryptovirus, family
Partitiviridae
White clover cryptic virus 2genus Betacryptovirus, family Partitiviridae
White clover mosaic virusgenus Potexvirus
Yam mild mosaic virusunclassified
Yam mosaic virusgenus Potyvirus, family Potyviridae
Zucchini yellow fleck virusgenus Potyvirus, family Potyviridae
Zucchini yellow mosaic virusgenus Potyvirus, family Potyviridae
SYMPTOMS OF CROP VIRUS DISEASES
Virus diseases are recognized by their symptoms. The important symptoms are: mosaic, chlorosis, mottle, yellows, ring spot, streak, stripe, vein
band, fleck, line pattern, vein chlorosis, vein clearing, necrosis, reddening,
browning, blackening, wilt, etch, leaf rolling, leaf curling, curly top, leaf
crinkling, leaf distortion, fern-leaf, malformations, rugose, enation, big
vein, rosette, tumors, bud blight, flower color-breaking, dwarf, stunt, and
sterility. These symptoms are described in Chapter 11.
MODE OF TRANSMISSION OF VIRUS DISEASES
Mechanical Transmission
Some viruses pass directly from plant to plant when their leaves rub together. Mechanical transmission will occur if virus concentrations in plant
sap and virus stability are high. Tobacco mosaic virus, Tomato mosaic virus,
Cucumber green mottle virus, Tobacco necrosis virus, and Potato virus X
are easily transmitted by mechanical contact. Viruses also may spread by
sticking on the clothes and hands of workers and equipment. For example,
mowing machines spread White clover mosaic virus and Red clover mottle
virus.
Transmission by Grafting
Grafting is a common practice in horticultural crops. Viruses may pass
through grafting. Citrus tristeza virus is transmitted by this method.
Transmission Through Vegetative Propagation Material
Viruses normally invade the host systemically and hence all plant parts
contain the virus. Rhizomes, tubers, corms, bud woods, and stem cuttings
are the common vegetative propagation materials and they may serve as
sources for virus infection. Banana bunchy top virus is transmitted mostly
through suckers. Potato virus Y, Potato virus X, and Potato virus T are transmitted through potato seed tubers.
Transmission Through Seed
Several viruses are found in immature seed coats, which consist of the integuments and nucellar remnants, and in the periplasm. Because the seed
coat and periplasm are part of the mother plant, they may become infected
due to systemic invasion by the viruses. Viruses do not survive desiccation,
and during maturation of the seed coat, they perish. Because there is no vascular contact between the embryo and mother plant, viruses cannot move to
the embryo from the seed coat. However, some viruses, such as Tobacco
mosaic virus, are highly stable, and the seed coat contamination with these
viruses may lead to seed transmission. However, this is rare.
Most seed transmission of viruses occurs through the embryo only. Both the
embryo and endosperm are formed within the embryo sac after fertilization
and lack direct connection and cellular contact with the mother plant through
plasmodesmata. Hence, a virus from the mother plant cannot move to the
embryo after fertilization. Embryo infection can occur only when the
mother plant is infected before the production of gametes or before cytoplasmic separation of embryonic tissue. Infection after flowering does not
lead to transmission through seed or pollen. Viruses that cannot pass beyond
the phloem (e.g., viruses that are transmitted by phloem-feeding insects)
will not be seedborne. The important viruses that are transmitted by seed
(Jackson et al., 1989; Mink, 1993; Johansen et al., 1994) include the following:
BarleyBarley stripe mosaic virus
BeanBean common mosaic virus, Bean pod mottle virus, Bean
southern mosaic virus, Bean yellow mosaic virus
Broad beanBroad bean mottle virus, Broad bean stain virus,
Broad bean wilt virus
CornMaize chlorotic dwarf virus, Maize dwarf mosaic virus,
Maize mosaic virus
CowpeaBlackeye cowpea mosaic virus, Cowpea aphidborne
mosaic virus, Cowpea mild mottle virus, Cowpea mosaic virus,
Cowpea severe mosaic virus
LettuceLettuce mosaic virus
LucerneLucerne latent virus, Lucerne transient streak virus
MelonMelon necrotic spot virus
MungbeanMungbean mosaic virus
MuskmelonMuskmelon necrotic ringspot virus
OatOat mosaic virus
PeaPea early-browning virus, Pea enation mosaic virus, Pea
seedborne mosaic virus
PeanutPeanut clump virus, Peanut mottle virus, Peanut stripe
virus, Peanut stunt virus
SoybeanSoybean mosaic virus
SunflowerSunflower mosaic virus
TobaccoTobacco etch virus, Tobacco mosaic virus, Tobacco rattle
virus, Tobacco ringspot virus, Tobacco streak virus
TomatoTomato aspermy virus, Tomato black ring virus, Tomato

bushy stunt virus, Tomato spotted wilt virus, Tomato ringspot virus
UrdbeanUrdbean leaf crinkle virus
WatermelonWatermelon mosaic virus
WheatWheat streak mosaic virus, Wheat striate mosaic virus
Transmission Through Pollen
Some viruses are known to be transmitted through pollens. Prunus necrotic ringspot virus and Prune dwarf virus in stone fruits, Blueberry shock
virus and Blueberry leaf mottle virus in blueberry, Cherry leafroll virus in
walnut, Raspberry bushy dwarf virus in raspberry, and Tobacco streak virus
in many crops are transmitted from plant to plant through pollens. Insects
may move virus-contaminated pollen to flowers on healthy plants. Pollenfeeding insects, such as thrips which thrive in the pollen receptors of flowers, may create wounds needed for the mechanical transmission of viruses
to nongametophytic tissues (Mink, 1993).
Transmission by Insects
Several insects transmit virus diseases. The transfer is not merely mechanical, and the relationship between virus and vector is complex. Some
mechanically transmissible viruses are not transmitted by insects. Mechanically transmitted viruses such as Tobacco mosaic virus, Potato virus X,
and White clover mosaic virus are not transmitted by insects. Virus transmission by insects is highly specific. Viruses transmitted by leafhoppers are
not transmitted by aphids. Strains of one virus may have different insect
species for their transmission. Insects have different kinds of mouthparts
and ways of feeding. Insects such as beetles and caterpillars have biting and
chewing mouthparts. They eat away the leaf pieces and damage the plants
severely. Cells that are touched by the insects mouthparts are destroyed. Viruses coming through these mouthparts cannot establish on the leaves, and
hence transmission is rare among the biters and chewers. However, some viruses, such as Southern bean mosaic virus, Broad bean stain virus, Radish
mosaic virus, Turnip yellow mosaic virus, and Cowpea mosaic virus, are
transmitted by beetles.
The majority of insects that transmit viruses have piercing and sucking
mouthparts. Aphids and leafhoppers, for example, have these kinds of
mouthparts. Aphids make initial, brief sap-sampling probes between epidermal cells or into them. However, aphids take up food from phloem vessels only. They need several minutes to hours to penetrate between plant
cells rather than through cells to finally reach the phloem. The mouthparts
of leafhoppers are most robust compared to aphids. They penetrate the plant
cells and reach the phloem within a few minutes. In the phloem, sap flows
under pressure and will pass up into the insects food canal. Hence, leafhoppers transmit viruses found in the phloem of infected plants. Thrips have
rasping and sucking mouthparts. They feed by grasping open the epidermal
cells or by rasping them and sucking up their contents. Thus, thrips can
transmit viruses found in epidermal cells also.
The virus-vector relationship varies widely depending upon the duration
of the virus in the vector (persistence). In the case of persistent viruses, the
virus may simply circulate through the body of the vector or it may propagate. Hence, this relationship can be classified as (1) noncirculative nonpersistent, (2) noncirculative semipersistent, (3) circulative nonpropagative,
and (4) circulative propagative transmission.
Noncirculative Nonpersistent Transmission
Aphids transmit viruses mostly from and into the hosts parenchyma.
The virus is acquired and inoculated during a brief feeding period of a few
seconds to some minutes, mostly during probes. Aphids become infective
immediately after virus uptake. Persistence in the vector is very brief. In exceptional cases, infectivity persists up to 40 hours when the vector has no
access to plants after acquiring the virus. These viruses are styletborne.
Hence, the transmission is noncirculative nonpersistent. The transmission is
of low specificity. Aphids, which are not pests and do not colonize on the
crop, but merely probe the surface for a palatable host, can also transmit the
viruses efficiently (Vidhyasekaran, 1993).
Circulative Nonpropagative (Persistent) Transmission
Viruses taken up by the vector enter the alimentary canal, pass through
the gut wall, circulate in body fluid (haemolymph), and contaminate the saliva. After a latent period, the insect becomes infective at the next feeding.
Infectivity is lost at moulting. Such uptake and inoculation happens only after long feeding times, usually after 15 minutes. Viruses are acquired from
the phloem of vascular bundles. Persistent transmission is associated with
highly specific virus-vector relationships. Certain viruses are spread by a
single vector species or a biotype, and even individuals may differ in efficiency as vectors. Leafhoppers generally transmit viruses in a persistent
manner.
Circulative Propagative (Persistent) Transmission

Certain viruses are propagated inside the insect body and may last until
death. Even transovarial transfer of the viruses through insect eggs is found
in leafhoppers. Wheat striate mosaic virus, Rice dwarf virus, and Rice stripe
virus are transmitted in this manner. Such viruses can persist in populations
without access to susceptible hosts. Once infective, the insects transmit the
virus to healthy plants throughout their life period.
Semipersistent Viruses
Semipersistent viruses can be transmitted mechanically with some difficulty. Viruses are taken up from the phloem by insects as they feed. The
viruses are adsorbed and gradually eluted from the line of pharynx. They are
then transmitted through ejection or egression at the start of subsequent
feeding. Beet yellows virus is transmitted this way.
Infection of Insect Vectors
Harmful effects of some plant viruses on insect vectors have also been reported. The planthopper Laodelphax striatellus, which transmits Rice stripe
virus, is infected by the virus itself. Individuals from eggs laid by infective
planthoppers die prematurely, if they hatch at all. The mortality of nymphs
is also high, particularly in the first and second instars.
The following is a list of viruses transmitted by various insects:
Aphids
Banana bunchy top virusPentalonia nigronervosa
Bean yellow mosaic virusMyzus persicae
Beet yellow net virusMyzus persicae
Beet yellow stunt virusNasonova lactucae
Beet yellows virusMyzus persicae, Aphis fabae
Bean common mosaic virusMyzus persicae
Broccoli necrotic yellows virusBrevicoryne brassicae
Carnation necrotic fleck virusMyzus persicae
Carrot latent virusSemiaphis heraclei
Carrot yellow leaf virusSemiaphis heraclei
Celery yellow spot virusHyadaphis foeniculi
Citrus tristeza virusToxoptera citricidus, Aphis gossypii
Clover yellows virusAphis craccivora
Cotton anthocyanosis virusAphis gossypii

Cucumber mosaic virusMyzus persicae
Groundnut rosette assistor virusAphis craccivora
Lettuce necrotic yellows virusHyperomyzus lactucae
Lucerne enation virusAphis craccivora
Pea enation mosaic virusAcrythosiphon pisum, Aulacorthum
solani
Pea seedborne mosaic virusMacrosiphum euphorbiae, Myzus
persicae, Acyrthosiphon pisum, Aphis craccivora,
Rhophalosiphum padi, Aphis fabae, Macrosiphum rosae,
Aulacorthum solani, Phorodon cannabis, Semiaphis dauci,
Brevicoryne brassicae
Peanut mottle virusMyzus persicae
Peanut stunt virusMyzus persicae
Physalis mild chlorosis virusMyzus persicae
Raspberry leaf curl virusAphis rubicola
Raspberry vein chlorosis virusAphis idaei
Sonchus yellow net virusAphis coreopsidis
Sowthistle yellow vein virusHyperomyzus lactucae
Soybean mosaic virusAphis craccivora, Myzus persicae
Strawberry crinkle virusChaetosiphon fragaefolii
Strawberry mild yellow edge virusChaetosiphon fragaefolii
Subterranean clover stunt virusAphis craccivora
Tobacco vein distorting virusMyzus persicae
Tobacco yellow net virusMyzus persicae
Tobacco yellow vein assistor virusMyzus persicae
Tomato yellow net virusMyzus persicae
Wheat yellow leaf virusRhopalosiphum padi
Leafhoppers
American wheat striate mosaic virusEndria inimica
Cereal chlorotic mottle virusNesoclutha pallida
Maize streak virusCicadulina mbila
Oat striate mosaic virusGraminella nigrifrons
Potato yellow dwarf virusAceratogallia sanguinolenta, Agallia
constricta
Rice transitory yellowing virusNephotettix apicalis
Tobacco yellow dwarf virusOrosius argentatus
Wheat dwarf virusPsammotettix alienus
Planthoppers
Barley yellow striate mosaic virusLaodelphax striatellus
Colocasia bobone disease virusTarophagus proserpina
European wheat striate mosaic virusJavesella dubia, J. pellucida
Maize mosaic virusPeregrinus maidis
Maize rough dwarf virusPeregrinus maidis
Maize sterile stunt virusSogatella kolophon, Peregrinus maidis
Northern cereal mosaic virusTerthron albovittatus, Ukanodes
sapporona, U. albifascia, Muellerianella fairmairei, Laodelphax
striatellus
Oat sterile dwarf virusDicranotropis hamata, Javesella discolor,
J. dubia, J. obscurella, J. pellucida
Rice black-streaked dwarf virusUkanodes sapporona,
U. albifascia, Laodelphax striatellus
Rice grassy stunt virusNilaparvata lugens
Rice hoja blanca virusSogatodes cubanus, S. oryzicola
Rice stripe virusUkanodes sapporona, U. albifascia, Laodelphax
striatellus
Sugarcane Fiji disease virusPerkinsiella saccharicida, P. vastatrix
Thrips
Impatiens necrotic spot virusThrips tabaci
Peanut bud necrosis virusThrips tabaci
Peanut yellow spot virusThrips tabaci
Tomato spotted wilt virusThrips tabaci, Frankliniella spp.
Watermelon silvery mottle virusThrips tabaci
Whiteflies
Bean golden mosaic virusBemisia tabaci
Cucumber yellows virusTrialeurodes vaporariorum
Mungbean yellow mosaic virusBemisia tabaci
Tomato golden mosaic virusBemisia tabaci
Tobacco leaf curl virusBemisia tabaci
Tomato yellow dwarf virusBemisia tabaci
Tomato yellow leaf curl virusBemisia tabaci
Tomato yellow mosaic virusBemisia tabaci
Mealybugs
Cacao swollen shoot virusDelococcus tafoensis, Dysmicoccus
brevipes, Ferrisia virgata, Maconellicoccus ugandae, Paracoccus
sp., Paraputo anomalus, Planococcoides njalensis, Planococcus
sp., Planococcus citri, Planococcus keyae, Pseudococcus
concavocerarii, Pseudococcus hargreavesi, Pseudococcus
longispinus, Tylococcus westwoodi
Grapevine virus APseudococcus longispinus
Beetles
Andean potato latent virusEpitrix sp.
Bean mild mosaic virusDiabrotica undecimpunctata, Epilachna
varivestis
Bean pod mottle virusCeratoma trifurcata, Diabrotica balteata,
D. undecimpunctata, Colaspis lata, Epicanta vittata
Bean rugose mosaic virusCerotoma ruficornis, Diabrotica
balteata, D. adelpha
Broad bean mottle virusAcalymma trivittata, Diabrotica
undecimpunctata, Colaspis flavida
Broad bean stain virusApion vorax, A. aestivum, A. aethiops,
Sitona lineatus
Cowpea mosaic virusEpilachna varivestis, Ceratoma arcuata,
C. atrofasciata, C. variegata, Diabrotica adelpha, Diabrotica
virgifera, Acalymma vittatum. Systena sp., Diphaulaca meridae
Maize chlorotic mottle virusOulema melanopa, Chaetocnema
pulicaria, Systena frontalis, Diabrotica undecimpunctata,
D. longicornis, D. virgifera
Radish mosaic virusEpitrix hirtipennis, Diabrotica
undecimpunctata, Phyllotreta striolata
Rice yellow mottle virusOulema dunbrodiensis, Cryptocephalus
chalybeipennis, Cryptocephalus nigrum, Monolepta flaveola,
Monolepta hamatura, Sesselia pusilla, Chaetocnema abyssinica,
Chaetocnema kenyensis, Chaetocnema pulla, Dactylispa bayoni,
Dicladispa paucispina, Dicladispa virdicyanae, Trichispa sericea
Southern bean mosaic virusCerotoma trifurcata, Epilachna
varivestis, Atrachya menetris, Acalymma vittata
Squash mosaic virusDiabrotica balteata, D. undecimpunctata,
D. longicornis, D. virgifera, Acalymma vittata, A. thiemei,
A. trivittata, Epilachna chrysomelina
Turnip yellow mosaic virusPhyllotreta spp., Psylliodes spp.,
Phaedon cochleariae
Lace Bugs
Beet leaf curl virusPiesma quadratum
Plant Bugs
Spinach blight virusLygus protensis
Earwigs
Turnip yellow mosaic virusFoficula auricularia
Lygacid Bugs
Centrosema mosaic virusNysius sp.
Flies (Leafminer)
Sowbane mosaic virusLiriomyza langei (mechanical transmission)
Tobacco mosaic virusLiriomyza langei (mechanical transmission)
Grasshoppers
Potato virus X virusMelanoplus differentialis
Treehoppers
Tomato pseudo-curly top virusMicrutalis malleifera
Transmission by Mites
Mites belong to the class Arachnida and order Acarina. Mites have puncturing and sucking mouthparts. Eryophid mites commonly transmit viruses.
Eryophid mites are elongate and tiny, about 0.2 mm long. They are invisible
to the naked eye and often hide between hairs of leaves and in buds. They do
not have much movement and are carried by the wind. They have slender
styles with which they puncture epidermal cells. The two pads at the tip of
the rostrum help in bringing saliva to the stylets and in sucking up plant sap.
Spider mites (Brevipalpus spp.) also transmit viruses. The important virus
diseases transmitted by mites include:
Agopyron mosaic virusAbacarus hystrix
Cherry mottle leaf virusPhytoptus inaequalis
Citrus leprosies virusBrevipalpus obovatus
Coffee ringspot virusBrevipalpus phoenicis
Fig mosaic virusEriophyes ficus
Onion mosaic virusEriophyes tulipae
Peach mosaic virusPhytoptus insidiosus
Ryegrass mosaic virusAbacarus hystrix
Wheat spot mosaic virusEriophyes tulipae
Wheat streak mosaic virusEriophyes tulipae
Transmission by Nematodes
Some nematodes belonging to the genera Longidorus, Xiphinema, Trichodorus, and Paratrichodorus are known to transmit viruses. Nematodeborne viruses are retained in the vector, including the guide-sheath lining of
the odontostyle in Longidorus, the lumen of the odontophore, the esophagus
in Xiphinema, and the entire pharynx and esophagus in Trichodorus. Nematodes, which have life spans of about two years, retain virus for long
periods. The following are viruses transmitted by nematodes:
Arabis mosaic virusXiphinema diversicaudatum, X. coxi,
X. bakeri
Brome mosaic virusXiphinema diversicaudatum
Carnation ringspot virusXiphinema diversicaudatum
Cowpea mosaic virusXiphinema basiri
Grapevine fanleaf virusXiphinema index, X. italae
Pea early-browning virusParatrichodorus pachydermus, P. teres,
P. anemones, Trichodorous viruliferus
Peach rosette mosaic virusXiphinema americanum,
Criconemoides xenoplax
Prunus necrotic ringspot virusLongidorus macrosoma
Raspberry ringspot virusLongidorus elongatus, L. macrosoma,
L. elongates
Strawberry latent ringspot virusXiphinema diversicaudatum,
X. coxi
Tobacco rattle virusParatrichodorus anemones, P. nanus,
P. pachydermus, P. teres, P. cylindricus, Trichodorus sparsus,
T. viruliferus
Tobacco ringspot virusXiphinema americanum

Tomato black ring virusLongidorus attenuatus, L. elongatus
Tomato ringspot virusXiphinema americanum, X. brevicolle
Transmission by Fungi (Including Protozoa)
Some soilborne fungal (including Protozoa) pathogens transmit plant viruses. Olpidium (belonging to Chytridiomycota, Fungi), Polymyxa, and
Spongospora (belonging to Plasmodiophoromycota, Protozoa) are pathogens
of crop plants. The zoospores of these fungi are released from the host, carry
the virus, and transmit it to susceptible hosts during the infection process. In
some cases, plant viruses are carried on the outside of the fungi. Some examples include Tobacco necrosis virus and Cucumber necrosis virus. Viruses such as Lettuce big vein virus are found inside the zoospores. These
viruses persist for years in viable resting sporangia. Transmission by fungi
can be of two types: nonpersistent and persistent transmission. The following are viruses transmitted by fungi, including protozoa:
Barley yellow mosaic virusPolymyxa graminis
Beet necrotic yellow vein virusPolymyxa betae
Cucumber necrosis virusOlpidium radicale
(= O. cucurbitacearum)
Lettuce big-vein virusOlpidium brassicae
Oat mosaic virusPolymyxa graminis
Potato mop-top virusSpongospora subterranea
Rice necrosis mosaic virusPolymyxa graminis
Soilborne wheat mosaic virusPolymyxa graminis
Tobacco necrosis virusOlpidium brassicae
Tobacco stunt virusOlpidium brassicae
Wheat mosaic virusPolymyxa graminis
Wheat spindle streak mosaic virusPolymyxa graminis
Wheat yellow mosaic virusPolymyxa graminis
Transmission Through Soil and Water
Some viruses, such as Tobacco mosaic virus and Tomato mosaic virus,
are highly stable and retain infectivity within soil for a long time. They may
survive in diseased plant debris and infect plants grown in infested soil.
Similarly, Cucumber green mottle mosaic virus survives in water. These viruses are transmitted mechanically (Vidhyasekaran, 1993).
Transmission Through Dodder

Dodder (Cuscuta sp.) is a chlorophyll-less parasitic plant. It grows and
entwines its yellowish stem around the stems of host plants. It produces haustoria, penetrates the hosts vascular bundles, and forms natural bridges between diseased and healthy plants. Viruses from diseased plants pass through
dodder stems and enter healthy plants. Most viruses can be transmitted
through dodder plants, although a natural transmission of virus diseases has
not been reported.
VIRUS DISEASE DIAGNOSIS
Virus disease diagnosis is important to plan for effective disease management. Several immunological methods have been developed to diagnose virus diseases, including agglutination tests, precipitation tests, immunodiffusion tests, enzyme-linked immunosorbent assays (ELISA), double
antibody sandwich assays (DAS-ELISA), imunofluorescent tests, and immunosorbent electron microscopy (ISEM). Many diagnostic methods based
on the nucleic acids of viral pathogens are also widely used, including restriction fragment-length polymorphisms (RFLPs), polymerase chain reactions (PCR), nucleic acid hybridization dot-blot tests, cDNA probes, reverse
transcription-PCR (RT-PCR), inverse PCR, arbitrarily primed PCR (APPCR), and DNA amplification fingerprinting (DAF) tests. For more information about these tests, see Chapter 13.
IMPORTANT CROP DISEASES CAUSED BY VIRUSES
The following are economically important virus diseases of major crops:
Banana bunchy top: genus Nanavirus, Banana bunchy top virus
(BBTV)
Barley yellow dwarf: genus Luteovirus, Barley yellow dwarf virus
(BYDV)
Barley yellow mosaic: genus Bymovirus, Barley yellow mosaic virus
(BaYMV)
Citrus tristeza: genus Closterovirus, Citrus tristeza virus (CTV)
Cucumber mosaic: genus Cucumovirus, Cucumber mosaic virus
(CMV)
Pea seedborne mosaic: genus Potyvirus, Pea seedborne mosaic virus
(PSbMV)
Peach necrotic ring spot: genus Ilarvirus, Prunus necrotic ringspot

virus (PNRSV)
Plum pox (= Sharka): genus Potyvirus, Plum pox virus (PPV)
Potato leafroll mosaic: genus Luteovirus, Potato leafroll virus
(PLRV)
Potato leafrolling: genus Carlavirus, Potato virus M (PVM)
Potato mild mosaic: genus Potyvirus, Potato virus A (PVA)
Potato mop-top: genus Furovirus, Potato mop-top virus (PMTV)
Potato rugose mosaic: genus Potyvirus, Potato virus Y (PVY, strains
O, N, and C)
Potato stem mottle (spraing disease): genus Tobravirus, Tobacco
rattle virus (TRV)
Potato virus X: genus Potexvirus, Potato virus X (PVX)
Rice grassy stunt: genus Tenuivirus, Rice grassy stunt virus (RGSV)
Rice tungro: genus Badnavirus, Rice tungro bacilliform virus (RTBV),
and genus Waikavirus, Rice tungro spherical virus (RTSV)
Tobacco mosaic: genus Tobamovirus, Tobacco mosaic virus (TMV)
Tomato mottle: genus Bigeminivirus, Tomato mottle virus (ToMoV)
Tomato spotted wilt: genus Tospovirus, Tomato spotted wilt virus
(TSWV)
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COMMON DISEASE SYMPTOMS,

ASSESSMENT, AND DIAGNOSIS
11
Common
Common
Disease
Disease Symptoms
Symptoms
Common names of diseases are always based on their key symptoms.
These symptoms are described here.
anthracnose: A characteristic lesion, which is a circular to angular, sometimes irregular sunken spot with grayish-black center and yellow margin in
leaves, stems, and fruit (e.g., grapevine anthracnose).
areolate mildew: Mildew growth in the area between veins on a leaf (e.g.,
cotton areolate mildew).
big vein: A condition in which veins become enlarged (e.g., Lettuce big
vein virus disease).
black scurf: Black flaky or scaly matter adhering to the surface of a plant
part (e.g., black scurf of potato).
blackening: Intensive necrosis that leads to blackening of tissues (e.g., Potato black ringspot virus disease).
blast: A disease that kills plants suddenly (e.g., rice blast).
blight: A plant disease characterized by withering and shriveling without
rotting (e.g., late blight of potato).
blister blight: A bubblelike elevation on the surface of a diseased leaf that
results in withering and shriveling of the leaf (e.g., tea blister blight).
boll rot: Decay of boll, a fruit of plants such as cotton. Boll consists of a
rounded capsule containing the seeds (e.g., cotton boll rot).
brown rot: A condition in which decaying tissues turn brownish (e.g., potato brown rot).
browning: Cell death leads to necrosis, which leads to the browning of tissues (e.g., Pea early browning virus disease).
bud blight: Necrosis of buds (Groundnut bud blight virus disease).
bud rot: Decay of buds (e.g., bud rot of coconut).

bunchy top: Leaves arise in clusters, giving a rosette appearance at the top
(e.g., Banana bunchy top virus disease).
bushy stunt: The diseased plant is severely stunted and its shoots are
crowded, giving a bushy appearance (e.g., Tomato bushy stunt virus disease).
canker: A corky outgrowth formed on leaves, twigs, and fruit (e.g., citrus
canker).
charcoal rot: Decaying tissues turn charcoal (black) in color (e.g., corn
charcoal rot).
chlorosis: Yellowing or whitening may be distributed in the entire plant due
to a partial failure of chlorophyll development in leaves and other plant
parts, causing more or less uniform discoloration (e.g., Tomato chlorosis virus disease, Lettuce chlorosis virus disease).
club root: Roots are malformed into clublike structures due to a thick,
fleshy growth of roots. The root tips are malformed, leaving the basal portions of the root mostly normal (e.g., club root of cabbage).
collar rot: Decay of the collar region of seedlings at the postemergence
stage (e.g., tobacco collar rot).
crown gall: Abnormal outgrowth or swelling produced due to hyperplasia
and hypertrophy of host cells (e.g., crown gall of several crops, such as apple, peach, and pear).
crown rot: Rotting of the crown that may spread to the root (e.g., oat crown
rot).
curly top: Inward rolling of the leaves with puckering and blisterlike elevation (e.g., Beet curly top virus disease).
damping-off: Rotting and collapse of seedlings at soil level or prevention
of seedling emergence (e.g., damping-off of vegetables and tobacco).
dieback: Death of diseased plant organs, especially stem or branches, from
the tip backward (e.g., eutypa dieback of grape).
downy mildew: Fungal growth that appears as a coating of soft, fine hair on
the surface of the host (e.g., downy mildew of grapevine).
dry rot: Putrefactive decomposition of tissues (e.g., dry root rot of citrus
and peanut).
dwarf: Plant growth is stunted due to disease (e.g., rice yellow dwarf disease).
ear rot: Decay of the ear of a cereal plant that contains the seeds, grains, or
kernels (e.g., corn diplodia ear rot).
enation: Small outgrowths on leaves, especially on veins and stems (e.g.,
Pea enation mosaic virus disease).
etch: Desiccation of superficial tissue (epidermal cells) (e.g., Tobacco etch
virus disease).
false smut: Individual grains of the panicle are transformed into greenish
spore balls. At first, the spore balls are smooth and yellow and are covered
by a membrane. When the membrane bursts, the color of the ball becomes
orange, yellowish green, or greenish black. The surface of the ball cracks
subsequently. When the balls are cut open, the innermost layer is yellowish,
the next layer is orange, and the outermost layer is greenish (e.g., false smut
of rice).
fern-leaf: Reduced development of the leaf blade in proportion to the
midrib leads to fern-leaf development (e.g., Asparagus fern-leaf virus disease).
fire blight: Diseased blossoms turn brown or black and exhibit a burnt appearance (e.g., fire blight of apple).
fleck: Small discolored parts are sharply bordered but circular (e.g., Parsnip
yellow fleck virus disease).
flower color-breaking: Mosaic or variegation of petals of flowers (e.g., Tulip color-breaking virus disease).
foot rot: Decay of the basal portion of a plant (e.g., rice foot rot).
freckle: A small brownish spot or small area of discoloration; a freckle is
smaller than a spot (e.g., banana freckle).
fruit rot: Decay of fruit (e.g., strawberry alternaria fruit rot).
gray mold: A condition in which infected blossoms and fruit become
coated with the fine gray fruiting stalks and spores of the fungal pathogen
(e.g., gray mold [Botrytis cinerea] of strawberry).
green ear: The floral parts of the ear of a cereal plant are transformed into a
green leafy structure; in the spikelet, the glumes, lemmae, paleae, stamens,
and pistil are all transformed into green leafy structures (e.g., green ear of
pearl millet).
gummosis: Secretion of gum as a symptom of the disease (e.g., citrus gummosis).

head rot: Decay of the head of plants such as sunflowers, which consists of
fertilized inflorescence with developed seeds and grains (e.g., sunflower
head rot).
kernel rot: Decay of kernels (e.g., corn fusarium kernel rot).
leaf blight: The entire leaf may be blighted (e.g., northern corn leaf blight).
leaf blotch: Irregular, large, discolored, usually black spots (e.g., leaf
blotch of barley).
leaf crinkle: Wrinkles form in the infected leaf (e.g., Turnip crinkle virus
disease).
leaf crumple: The infected leaf appears crushed, forming wrinkles or
creases (similar to marks produced by folding) (e.g., cotton leaf crumple).
leaf curl: Leaf margins turn downward and come together at the bottom, exposing the middle upper surface of the leaf blade; the disease results in the
downward curling of leaves; the distortion, puffing, and crinkling of a leaf
resulting from the unequal growth of its two sides (e.g., Tobacco leaf curl
virus disease).
leaf roll: A condition in which the leaf tends to roll (e.g., Potato leafroll virus disease).
leaf spots: Several types of spots are seen in infected leaves. Water-soaked
spots are common in bacteria-infected leaves; circular, cylindrical, irregular, or angular spots are common in fungus and bacteria-infected leaves
(e.g., sigatoka leaf spot of banana).
line pattern: Infected leaves show an arrangement of repeated lines (e.g.,
Pelargonium line pattern virus disease).
little leaf: In diseased plants, the leaves are malformed into tiny chlorotic
structures (e.g., little leaf of eggplant).
mosaic: Infected leaves show various shades of green and yellow areas that
are usually irregularly angular, but sharply delimited. Mosaic is characterized by a patchy variation of normal green color (e.g., Tobacco mosaic virus
disease).
mottle: Diffusely bordered variegation (differently colored spots), i.e., a
pattern of white patches in leaves and other plant parts, that results from the
failure of chlorophyll to develop in certain cells. Mottle is an arrangement of
spots or confluent blotches of different shades, as of the surface of marble

(e.g., Tomato mottle virus disease, Bean pod mottle virus disease).
necrosis: A symptom in which cells die in patches (e.g., Rice necrosis virus
disease).
phyllody: The floral parts are transformed into green leafy structures (e.g.,
phyllody of sesamum).
powdery mildew: Fungal growth seen as a powdery growth on the hosts
surface (e.g., wheat powdery mildew).
Pox: Infected leaves show various types of mottling, which consist of light
green to yellowish-green blotches. The fruits are poxed (i.e., pustules form
on the skin) (e.g., Plum pox virus disease).
red rot: Decaying tissues appear reddish in color (e.g., sugarcane red rot).
reddening: Red pigments predominate (e.g., Carrot red leaf virus disease).
rhizome rot: Decay of rhizome (e.g., turmeric rhizome rot).
ringspot: Infected leaves show ringlike circular spots (e.g., Groundnut
ringspot virus disease, Carnation ringspot virus disease, Citrus ringspot virus disease).
root rot: Root decay leads to wilting in plants. In the field, a sudden and
complete wilting of the plants is seen. The major difference between wilt
and root rot is the discoloration of the stem. When the bark of plants is removed, black streaks can be seen extending upward to the branches and
downward to the lateral roots. Root rot, which also leads to wilting, is characterized by root decay (e.g., corn pythium root rot, cotton black root rot).
rosette: Rosetting (decoration resembling a rose) is formed due to impeded
internodal expansion at the stem tips; crowding of the foliage excessively in
the form of a rosette (e.g., Groundnut rosette virus disease).
rot diseases: Decay of tissues. Several types of rot symptoms are seen in
crops.
rugose: The growth of veinal tissue is retarded (e.g., Bean rugose mosaic
virus disease).
rust: Rustlike pustules (small elevated spots resembling pimples). Several
types of rust diseases are known and they are referred to by the color of their
pustules: Brown rust (brown [leaf] rust of wheat), black rust (black [stem]
rust of wheat), yellow rust (yellow [stripe] rust of wheat), and white rust
(white rust of crucifers). When pustules are arranged in linear rows between
the veins of the leaf, the rust is called stripe rust (stripe rust of wheat). In
the case of leaf rust, rust pustules are seen mostly on leaves (leaf rust of
wheat). In the case of stem rust, rust pustules are seen mostly on stems
(stem rust of wheat).
scab: The surface of affected tissues becomes rough and the affected surface is raised due to an abnormal proliferation of cells in the epidermis. The
surface has a rusty appearance that is deeply pitted with corky wounds (e.g.,
apple scab).
scald: Affected plants show lesions, which appear to be similar to the scalding caused by hot water. Such lesions are mainly bleached and may be partly
translucent (e.g., barley scald).
shot hole: Leaf spots in which the necrotic (spot) regions drop out, leaving
holes in the affected leaves (e.g., peach Stigmina shot hole).
silver leaf: A condition in which infected leaves show a metallic luster (e.g.,
plum silver leaf).
smut: Black sooty masses of fungal spores that cover the affected plant
parts. Several types of smuts are seen, including covered kernel smut: smut
sori replacing grains of plants (e.g., covered kernel smut of sorghum); long
smut: smut sori are covered by a fairly thick membrane and are much longer than the other smuts; they are cylindrical in shape (e.g., long smut of
pearl millet); loose smut: smut sori are covered with a fragile membrane,
which breaks easily at the time of spike emergence from the host, exposing a
powdery mass of spores (e.g., loose smut of wheat); covered smut: smut
sori are covered with a thick membrane, which resists easy rupturing (covered smut of oats); flag smut: smut sori occur on the leaf blade, leaf sheath,
and culm (flag smut of wheat); head smut: the entire earhead is converted
into smut sori (head smut of corn); kernel smut: symptoms appear only on
mature grains; minute black pustules or streaks bursting through the glumes
are seen; sometimes the entire grain is replaced by a powdery, black mass of
smut spores (e.g., kernel smut of rice); and leaf smut: smut sori are formed
in infected leaves (leaf smut of rice).
soft rot: Soft, water-soaked, irregular lesions appear on tubers, rhizomes,
fruits, vegetables, and other storage organs. These lesions are more or less
superficial, but soon spread and cover the inner tissues. Lesions lead to rotting of storage organs (e.g., soft rot of potato).
sooty mold: Masses of fungal black spores that stick to the leaf surface,
making the foliage appear black and ugly (e.g., sooty mold of citrus, mango,
sapota, and guava).
spindle tuber: A condition in which the tubers of affected plants are elongated (spindle shaped) (e.g., Potato spindle tuber viroid disease).
spotted wilt: Bronze markings appear on the upper surface of young leaves,
and the markings extend from the leaf blade down to the petiole and stem,
resulting in wilting of the stem (e.g., tomato spotted wilt).
stalk rot: Decay of plant stalks (e.g., corn bacterial stalk rot).
stem bleeding: Exudation of sap or resin from an infected stem (e.g., coconut stem bleeding).
stem gall: Abnormal outgrowth in the stem (coriander Protomyces stem
gall).
stem rot: Decay of stem tissues (e.g., rice stem rot, crucifers sclerotinia
stem rot).
stenosis: In diseased plants, the leaves are highly reduced in size and clustered along the stem (e.g., cotton stenosis).
sterility: Suppression of development of reproductive structures. Diseased
plants do not produce seeds, fruit, stamens, or pistils (e.g., pigeonpea sterility mosaic disease).
storage rot: Decay of storage organs (e.g., corn aspergillus ear and storage
rot).
streak: Mosaic in leaves along veins, which looks like a streak (e.g., Tobacco streak virus disease).
stripe: A long band of mosaic pattern along the parallel veins of a leaf (e.g.,
Peanut stripe virus disease).
stunt: Retardation of plant growth due to disease (e.g., Peanut stunt virus
disease).
tumors: Swellings on stems or roots (e.g., Sweet clover root tumor virus
disease).
vein band: A broad dark-green band along the veins. The rest of the lamina
surface shows chlorosis (e.g., Strawberry vein banding virus disease).
vein chlorosis: Chlorosis is restricted to tissues adjoining the veins in
leaves (e.g., Raspberry vein chlorosis virus disease).
vein clearing: A clearing or chlorosis of the tissue in or immediately adjacent to the vein (e.g., Petunia vein-clearing virus disease).
wilt: In the early stages of wilting, yellowing of leaves is seen. A flaccid or

drooping condition of the plant due to disease, wilting may be due to a shortage of water, an impeded supply of nutrients, or excessive transpiration
(e.g., cotton fusarium wilt, banana fusarium wilt, crucifers verticillium
wilt).
witches-broom: Abnormal proliferation (mass outgrowths) of the branches
of woody plants due to disease (e.g., witches-broom of potato).
yellows: Uniform yellow to almost-white discoloration of leaves; yellowing
will be the conspicuous symptom in the diseased plant. Yellow pigments
may predominate in infected plants (e.g., crucifers aster yellows, Beet yellows virus disease).
12
CropCrop
Disease
Disease Assessment
Assessment
Crop disease assessment is otherwise called phytopathometry. It involves
the measurement and quantification of crop diseases. Accurate disease assessment will help in predicting the development of epidemics and in developing a decision support system for timing the application of fungicides to
control diseases. Diseases are assessed by different methods, based on the
type of disease symptoms and their relationship with yield loss. Remote
sensing and image analysis are important tools in disease assessment.
DISEASE INCIDENCE ASSESSMENT
A disease is assessed either as a percentage of disease incidence or as disease severity. Disease incidence is calculated as follows:
Percentage disease incidence =
(number of infected plants/total number of plants assessed)
100
Assessment of disease incidence will be useful for measuring systemic infections, which may result in total plant loss. Virus diseases such as rice
tungro, barley yellow dwarf, and banana bunchy top, and fungal diseases
such as loose smut of wheat and barley and sugarcane smut are assessed by
estimating disease incidence. Some fungal pathogens may not cause systemic infection, but may cause total crop loss. Wilt diseases such as
Fusarium wilt of tomato, Panama wilt of banana, and Fusarium wilt of
chickpea cause total losses and these diseases are assessed as percentage of
disease incidence. Monosporascus wilt of melons is assessed as percentage
of wilt incidence (Cohen et al., 2000).
Percentage of disease incidence is calculated also for some leaf spot and
fruit spot/rot diseases. Brown spot of pear, which is caused by Stemphylium
vesicatarium, is assessed by recording the number of leaves that show leaf
spots out of ten leaves of four shoots per tree (Llorente et al., 2000). The
brown spot disease incidence was also recorded as a percentage pear fruit
infection (Llorente et al., 2000). Diseases such as damping-off and some
root rots in which total losses occur are assessed by percentage of disease incidence.
DISEASE SEVERITY ASSESSMENT
Disease severity is generally calculated as follows (Cooke, 1998):
Disease severity = (area of diseased tissue/total tissue area)
100
Disease severity is assessed by allotting a grade value (category value or

scale value) depending upon the area of infection. The severity scale is
fixed, mostly based on the diseases effect on yield loss. Disease severity is
usually assessed using either descriptive or pictorial keys. Standard diagrams illustrate the developmental stages of a disease on simple units (e.g.,
leaves, fruits) or on large composite units such as branches or whole plants.
Such standard diagrams are derived from a series of disease symptom pictures, which may be in the form of line drawings, photographs, or preserved
specimens (Cooke, 1998). Pictorial disease assessment keys are available
for measuring disease severity on a range of hosts using the principle of
standard area diagrams (Cooke, 1998). Standard area diagrams are prepared
using graph paper outlines. Planimeters, electronic scanners, and image
analyzers are used to assess the area of infection. Lesion areas can be determined by computer-assisted image analysis (Timmer et al., 2000).
Disease severity is assessed by using arbitrary categories. Horsfall and
Barratt (1945) proposed a logarithmic scale for the measurement of plant
disease severity, in which 12 grades were allotted according to the leaf area
diseased, keeping 50 percent as a midpoint. These categories were developed taking into account the fact that the human eye apparently assessed
diseased areas in logarithmic steps, as stated by the Weber-Fechner law for
visual acuity (for appropriate stimuli, visual response is proportional to the
logarithm of the stimulus). This law has several steps in the scale around 50
percent infected area and decreasing steps at both ends of the scale. However, many modifications have been made to this scoring system, mostly
based on the possible yield loss due to each grade and each type of symptom. Grading systems consisting of five grades (1, 2, 3, 4, and 5 or 0, 1, 2, 3,
and 4) or nine grades (1, 3, 5, 7, and 9 or 0 to 9) are commonly used. Citrus
greasy spot caused by Mycosphaerella citri was assessed using a 0-to-5
scale (Timmer et al., 2000). Greasy symptoms on the top ten leaves of each
plant were rated on a scale of
0 = none
1 = 1 to 5 percent
2 = 6 to 10 percent
3 = 11 to 15 percent
4 = 16 to 20 percent
5 = more than 20 percent
of the leaf surface area affected by the disease (Timmer et al., 2000).
Monosporascus disease in cucumber was assessed using a 1-to-5 scale
(Bruton et al., 2000) as follows: 1 = healthy with no lesions or discoloration
on hypocotyls, 2 = slight discoloration, 3 = moderate discoloration and/or
with lesions, 4 = moderate maceration, and 5 = severe maceration. Bacterial
spot (Xanthomonas campestris pv. vesicatoria) of bell pepper was assessed
using a 0-to-9 rating scale in which
0 = no diseased leaves
1 = <1 percent leaf area diseased
2 = 2 to 10 percent leaf area diseased or defoliated
8 = 81 to 99 percent leaf area diseased and very few leaves (one to
three) remaining on plant
9 = complete defoliation (plant dying or dead) (Romero et al.,
2001)
Foliar symptoms of sudden death syndrome of soybean caused by Fusarium solani f. sp. glycines are assessed on a 0-to-9 scale based on (different
types of symptoms) the percentage of leaf area that is chlorotic, necrotic, or
defoliated as follows (Luo et al., 2000):
0 = no detectable leaf symptoms
1 = 1 to 10 percent chlorotic or 1 to 5 percent necrotic
2 = 10 to 20 percent chlorotic or less than 10 percent necrotic

5 = greater than 60 percent chlorotic or greater than 40 percent
necrotic
6 = up to one-third premature defoliation
7 = one-third to two-thirds premature defoliation
8 = greater than two-thirds premature defoliation
9 = plants are prematurely dead
Brown spot of pear was measured by calculating the severity of infection.
Measurements were performed on ten leaves of four shoots per tree. Disease
severity per shoot was calculated according to a scale index based on an approximate lesion number per leaf corresponding to 0 (none), 1 (1 to 5 lesions), 2 (6 to 25 lesions), and 3 (more than 25 lesions) (Llorente et al.,
2000).
Shaner and Buechley (1995) developed a leaf blotch severity scale based
on the position of leaves of wheat plants. The top leaves, particularly the top
two leaves, contribute to grain yield in cereals. The scoring system was
based on corresponding yield loss. Hence, Shaner and Buechley (1995)
scored the top four leaves (flag leaf, flag leaf-1 [the leaf immediately below
the flag leaf], flag leaf-2 [the leaf below flag leaf-1], and flag leaf-3 [the leaf
below flag leaf-2]) of tillers into different category values. Flag-3 leaves
with 0 to 5 percent severity were given the scale value of 1, 5 to 20 percent
(scale value 2), 20 to 40 percent (scale value 3), 40 to 70 percent (scale value
4), 70 to 90 percent (5), 90 to 100 percent (scale value 6), and 100 percent
(scale value 7). Contrarily, flag-2 leaves with 1 to 10 percent disease severity were given the scale value of 4, 10 to 25 percent (scale value 5), 25 to 75
percent (scale value 6), 75 to 100 percent (scale value 7), and 100 percent
(scale value 8). Flag-1 leaves with 0 to 1 percent disease severity were given
scale value of 5, 1 to 10 percent (scale value 6), 10 to 50 percent (scale value
7), 50 to 90 percent (scale value 8), 90 to 100 percent (scale value 9), and
100 percent (scale value 9.5), and flag leaves with 1 to 20 percent disease severity were given a scale value of 8, 20 to 90 percent (scale value 9), and 90
to 100 percent (scale value 9.5). Mean severity was calculated from the average of the four leaves of each tiller, based on midpoint values for each
range. Mean severity (P) is calculated from the scale value (S) according to:
P = 0.38253 0.69435 S + 1.17499 S 2 (R2 = 0.999).
Disease severity is also calculated by measuring the height up to which
infection spreads in an infected plant (vertical disease progress). Rice
sheath blight (Rhizoctonia solani) disease intensity is measured using a relative lesion height percentage as follows: The highest point a lesion is seen
in a plant (cm)/plant height 100 (Vidhyasekaran et al., 1997). In this case,
it has been shown that the relative lesion height corresponds to yield loss.
DISEASE INDEX
Data from both percentage of disease incidence and disease severity with
different scale values are often used to assess the disease index (Llorente
et al., 2000; Luo et al., 2000). Disease index is calculated as follows: Disease incidence percentage Disease severity scale the maximum severity
scale (Luo et al., 2000). Disease index is also calculated as the mean grade
value (totaling grade values of all the examined plants and dividing them by
number of the examined plants) 100 maximum grade value (Cooke,
1998). A brown spot of pear disease index was calculated by adding the
scale index of each leaf in a tree and dividing the sum obtained by 3 (the
maximum level of severity) and the number of leaves assessed per shoot
(Llorente et al., 2000). The disease scale can be recalculated to a percentage
severity value called the disease index (Nilsson, 1995).
AREA UNDER DISEASE PROGRESS CURVE (AUDPC)

Disease severity is also measured by calculating the area under disease
progress curve (Romero et al., 2001). Bacterial spot (Xanthomonas axonopodis pv. vesicatoria) of bell pepper was assessed by using a 0-to-9 rating
scale. The AUDPC was then calculated (Romero et al., 2001). For the procedure to calculate AUDPC, see Chapter 14.
REMOTE SENSING
Another disease assessment method involves aerial photography and
photogrammetry using infrared film or color filter combinations. Remote
sensing and digital image analysis are methods of acquisition and interpretation of measurements of an object without physical contact between the
measuring device and the object. The term remote sensing is restricted to instruments that measure electromagnetic radiation reflected or emitted from
an object (Nilsson, 1995). The instruments record radiation in various parts
of the electromagnetic spectrum: Ultraviolet (UV, 10-390 nm), visible (390700 nm), near infrared (NIR, 770-1300 nm), infrared (IR, 1300-2500 nm),
etc. The human eye records three visual spectral ranges: Red, green, and
blue. However, sensitivity to red over 650-700 nm is only slight. The human
eye cannot detect the infrared spectrum. The amount of reflected light (radiance) as a percentage of incoming light (irradiance) is called the reflectance
factor. If the radiance from a healthy leaf is measured by a radiometer, substantially high reflectance can be seen in NIR at 750-1100 nm. NIR reflectance decreases greatly when a diseased leaf is viewed due to the reduction
in chlorophyll and xanthophylls caused by infection (Nilsson, 1995). A relative decrease in NIR reflectance may indicate the disease severity. Aerial
photography using infrared film helps to detect disease incidence.
Remote sensing for detecting and estimating the severity of plant diseases is used at three levels above the crop canopy (Cooke, 1998). Handheld
multispectral radiometers or multiple waveband video cameras are used at
the lowest altitude (within 1.5 to 2.0 meters above crop height). At 75 to
1500 m, aerial photography is used. At the highest altitude, satellite imagery
is employed utilizing satellites orbiting 650 to 850 km above the Earths surface. Handheld multispectral radiometers or multiple waveband video cameras are useful to assess diseases in the greenhouse and in the field. Aerial
infrared photography is useful at field level. Satellite imagery is used for
disease surveillance in large areas or regions of the Earth. Aerial IR photography was successfully used in the 1970s for surveillance of southern corn
blight (Helminthosporium maydis) epidemic in the United States.
DIGITAL IMAGE ANALYSIS
Digital image analysis is being used to assess various plant diseases. Digital image analysis includes analysis of satellite images, aerial photographs
and videographs, nucleic magnetic resonance images, and images in electron microscopy. Image processing reduces the total information to a manageable amount. Aerial IR color photographs can be enhanced and colorcoded to facilitate visual interpretation of the distribution and severity of
diseases in fields (Nilsson, 1995). Blazquez and Hedley (1986) made computer-aided spectrophotometric measurements of 35 mm color IR film of
late blight infection in tomato fields. This technology has vast potential for
making rapid assessments of plant diseases.
REFERENCES
Blazquez, C. H. and Hedley, L. E. (1986). Late blight detection in tomato fields with
35 mm color infrared aerial photography. Phytopathology, 76:1093.
Bruton, B. D., Garcia-Jimenez, J., Armengol, J., and Popham, T. W. (2000). Assessment of virulence of Acremonium cucurbitacearum and Monosporascus cannonballus on Cucumis melo. Plant Dis, 84:907-913.
Cohen, R., Edelstein, M., Pivonia, S., Gamliel, A., Burger, Y., and Katan, J. (2000).
Toward management of Monosporascus wilt of melons in Israel. Plant Dis,
84:496-505.
Cooke, B. M. (1998). Disease assessment and yield loss. In D. G. Jones (Ed.), The
Epidemiology of Plant Diseases. Kluwer Publishers, Dordrecht, The Netherlands, pp. 42-71.
Horsfall, J. G. and Barratt, R. W. (1945). An improved grading system for measuring plant disease. Phytopathology, 35:655.
Llorente, I., Vilardell, P., Bugiani, R., Gherardi, I., and Montesinos, E. (2000).
Evaluation of BSPcast disease warning system in reduced fungicide use programs for management of brown spot of pear. Plant Dis, 84:631-637.
Luo, Y., Hildebrand, K., Chong, S. K., Myers, O., and Russin, J. S. (2000). Soybean
yield loss to sudden death syndrome in relation to symptom expression and root
colonization by Fusarium solani f. sp. glycines. Plant Dis, 84:914-920.
Nilsson, H.-E. (1995). Remote sensing and image analysis in plant pathology. Annu
Rev Phytopathol, 15:489-527.
Romero, A. M., Kousik, C. S., and Ritchie, D. F. (2001). Resistance to bacterial spot
in bell pepper induced by acibenzolar-S-methyl. Plant Dis, 85:189-194.
Shaner, G. and Buechley, G. (1995). Epidemiology of leaf blotch of soft red winter
wheat caused by Septoria tritici and Stagonospora nodorum. Plant Dis, 79:928938.
Timmer, L. W., Roberts, P. D., Darhower, H. M., Bushong, P. M., Stover, E. W.,
Peever, T. L., and Ibanez, A. M. (2000). Epidemiology and control of citrus
greasy spot in different citrus growing areas in Florida. Plant Dis, 84:1294-1298.
Vidhyasekaran, P., Ruby Ponmalar, T., Samiyappan, R., Velazhahan, R., Vimala,
R., Ramanathan, A., Paranidharan, V., and Muthukrishnan, S. (1997). Hostspecific toxin production by Rhizoctonia solani, the rice sheath blight pathogen.
Waliyar, F., Adamou, M., and Traore, A. (2000). Rational use of fungicide applications to maximize peanut yield under foliar disease pressure in West Africa.
Plant Dis, 84:1203-1211.
13
CropCrop
Disease
Disease Diagnosis
Diagnosis
Disease diagnosis is very important for developing effective strategies
for disease management. Without diagnosis, there can be no disease management. Crop disease diagnosis is an art as well as a science (Holmes et al.,
2000). The diagnostic process involves the recognition of symptoms (which
are associated with disease) and signs (which are not outwardly observable)
and requires intuitive judgment as well as the use of scientific methods. Several conventional techniques are followed to diagnose disease incidence.
These techniques include visual inspection and recognition of symptoms
and isolation and examination of crop pathogens using microscopy. Such
techniques are time-consuming and may not be able to detect latent infections. Several diagnostic assays have been developed for early and rapid diagnosis. These include immunoassays, nucleic acid probe-based methods,
and PCR-based techniques. The use of these techniques in the diagnosis of
fungal, bacterial, viral, viroid, and phytoplasma diseases is described here.
DIAGNOSIS BASED ON DISEASE SYMPTOMS
Some pathogens produce characteristic symptoms that can be easily recognized in the field. Fungal diseases such as powdery mildews, rusts,
downy mildews, and smuts show characteristic symptoms. Some bacterial
diseases such as canker and crown gall can be recognized based on symptoms. A few virus diseases produce characteristic symptoms such as bunchy
top, rosette, witches-broom, phyllody, and flower color-breaking. Diseases
should be diagnosed at early stages of their development, so that epidemics
can be effectively prevented. The early stages of many diseases are inconspicuous and it may not be feasible to make a rapid visual assessment until
the level of disease is sufficiently high. Further, even at advanced stages of
symptom development, some diseases cannot be recognized with certainty.
For example, symptoms such as chlorosis, mosaic, leaf drooping, yellowing, dwarfing, stunting, necrosis, root rots, wilts, fruit rot, dieback, leaf
blight, and bud rot can be caused by several types of fungal, bacterial, viral
and phytoplasma pathogens. In these cases, the appearance of fruiting bod-
ies of the pathogens on leaf, stem, and fruit surfaces must be checked. However, sporulation of the pathogen occurs only at a very late stage of infection.
Sometimes saprophytes may develop on these lesions, making diagnosis a
difficult task. Hence, the pathogen has to be isolated in pure culture and
identified using a microscope. Sometimes selective media may be required
to isolate pathogens and induce sporulation for identification. Many fungal
pathogens grow slowly in media and may be lost too readily for identification. If the pathogen is a bacterium, virus, or phytoplasma, more complicated tests have to be conducted to identify them. Several biochemical tests
must be conducted to identify bacterium. Routines for extraction and purification of viruses require professional proficiency and skill. In fact, conventional plant-pathological techniques need high expertise for routine identification. In the case of latent infections in vegetative planting materials,
seeds, and fruit, conventional methods may not be useful to diagnose infection.
DIAGNOSIS BY IMMUNOLOGICAL TECHNIQUES
Immunodiagnostic assays provide a fast method of confirming visible
symptoms as well as detecting pathogens that cannot be easily identified by
other methods. They permit early detection of plant pathogens and accurate
identification of pathogens. Because many fungicides are specific only to
certain pathogens or groups of pathogens, immunodiagnosis will be useful
in the selection of the most appropriate treatment. Viruses, bacteria, and
fungi (especially those spreading as a sterile mycelium) can be readily detected by these methods.
Immunoassays depend on the development of antibodies specific to the
particular pathogen. Cells of living animals, particularly mammals, have the
ability to recognize binding sites on proteins, glycoproteins, lipopolysaccharides, and carbohydrate molecules that are not present in their bodies
(i.e., foreign to that animal). Such molecules, known as antigens, stimulate
the immune system of the animal and this leads to the production of specific
antibodies, each of which specifically recognizes and binds to its complementary antigen. The role of an immunoassay is to reveal the presence of
specific complexes between the antibody and an antigen that are unique to
the pathogen (Fox, 1998). The antibodies produced in an animal body can
recognize the microbial antigen, which is present on cell walls or found attached with them. In other words, the antibodies can recognize the plant
pathogen by recognizing the antigen specific to the pathogen. In principle,
immunoassays are based on the fact that antibodies react specifically with
the homologous antigen. However, the reaction is not easy to detect. Several
techniques have been developed to exploit this reaction in immunoassays.
Production of Polyclonal Antibodies
Rabbits, rats, mice, sheep, or goats are used to produce antibodies. For
the production of antibodies to fungal pathogens, mycelia (obtained from
sterile cultures grown in the laboratory) are freeze dried. They are then finely
ground with a pestle and mortar and used as antigens. Surface washings
from fungal mycelia are also used as antigens. These antigens are emulsified
with an equal volume of an adjuvant, such as Freunds complete adjuvant
for the first injection and Freunds incomplete adjuvant for the second or
subsequent injections. Other types of adjuvants such as Quil A are also
used. Bacterial antigens can be prepared by first killing the bacteria with
heat or formalin and then suspending them in phosphate-buffered saline.
The bacterial cells are disrupted by grinding, sonication, or enzyme action
to produce soluble cytoplasmic antigens. Virus antigens can be prepared
from infected host tissues by differential centrifugation or precipitation with
ammonium sulfate.
These antigens (otherwise called immunogens) are then injected into
rabbits (or other animals) to induce complementary antibodies. The antigen
emulsion is injected under the skin at the back of the neck of the animals.
One or more booster injections with the emulsion are given several weeks
after first injection. Antiserum is obtained by taking blood from the ears of
rabbits or from the tail veins of mice.
Antibodies have a basic common structure, which consists of two heavy
and two light chains held together by disulfide bonds. However, they differ
in the ability of their tips to bind to sites on different foreign protein,
glycoprotein, lipoprotein, lipopolysaccharide, and carbohydrate molecules
(antigenic substances). Such antigenic substances stimulate the animals
immune systems to produce an assortment of specific antibodies, each of
which specifically recognizes and binds almost exclusively to its complementary epitope (antibody reaction site) on the antigen. This interaction between an antibody and an antigen depends on pairing of an individual
epitope of the antigen in three dimensions between the contours of the tips
of the side chains of a particular antibody. Several antigens have been detected in viral, bacterial, and fungal pathogen preparations. The antisera
raised against these antigens may contain a mixture of antibodies directed
toward various epitopes of the pathogens. These antisera, which contain
polyclonal antibodies, are called polyclonal antisera.
Production of Monoclonal Antibodies

Kohler and Milstein (1975) were the first to produce monoclonal antibodies. Monoclonal antibodies (MCAs) recognize a single epitope only and
can therefore differentiate between related pathogens. Monoclonal antibodies that are specific at genus, species, pathovar, and strain levels are available. MCAs are homogenous and therefore free from the variability common to polyclonal antisera. Once a hybridoma cell line is obtained, an
endless supply of the same MCA can be produced. The method of preparation of monoclonal antibodies is given here:
An antigen (immunogen) is prepared from cultures of fungal and bacterial pathogens or from the sap of virus-infected plants as described for the
preparation of polyclonal antibodies. Mice, usually females of the BALB/c
strain, are immunized by intraperitoneal injection with the antigen preparation. The preparation is given at about two week intervals until antiserum
from trial bleeds gives a high titer against the immunogen. The final booster
injection is given without an adjuvant about three to four days before fusion.
The mice are killed and their spleens are removed. -Lymphocytes are isolated from the spleens of immunized mice. Meanwhile, a potentially immortal line of myeloma cells is obtained from an animal similar to the animal used for immunization (mostly mouse), subcultured, and grown for
fusion with the spleen cells to form hybridoma cells. Viable spleen cells are
mixed at a ratio of 1:10 with viable myeloma cells. This cell mixture is
washed by centrifugation at 60 g for 10 min and the pellet loosened by tapping the tube. One ml of polyethylene glycol 4000 (PEG 4000) is then
added dropwise over a period of 1 minute to the pellet, stirred for another
minute, and slowly diluted by the addition of a tissue culture medium.
Dulbeccos modified Eagles medium is generally used as a tissue culture
medium. Penicillin-streptomycin solution, L-glutamine (200 mM), phenolred solution, and controlled processed serum replacement are added to the
medium. The cells are washed and resuspended in fresh HAT (hypoxanthine,
aminopterin, thymidine) medium (a selective medium made by supplementing the complete tissue culture medium with 0.01 M hypoxanthine, 0.0016 M
thymidine, and 0.00004 M aminopterin) and dispensed into 96-well tissue
culture plates. The plates are kept in an incubator at 37C with 5 percent
CO2. After ten to 16 days, aliquots of the supernatant are removed from the
wells containing clones and screened by ELISA (enzyme-linked immunosorbent assay). Only hybridomas producing antibodies specific to the antigen are saved and grown on a large scale in vitro. Selected hybridoma cells
are grown in bulk. The antibodies are purified on a sepharose column. Once
a useful MCA has been generated, the selected hybridoma cells can be im-
mortalized by storing them in liquid nitrogen. Whenever needed, MCAs can

be produced indefinitely and in unlimited quantities.
Both polyclonal and monoclonal antibodies are used in various immunodiagnosis techniques.
Agglutination Test
This test can be carried out in slides or in test tubes. In the slide agglutination test, drops of antigen and diluted antiserum containing antibodies are
mixed together on a glass microscope slide. Agglutination is observed by
eye or microscope (if ambiguous). In the test-tube agglutination test, the antigens are mixed with antibodies in test tubes, and the aggregation of antigens and antibodies is monitored with a binocular microscope.
Precipitation Test
In this test, aliquot dilutions of antigen are layered over equal volumes of
antiserum diluted in normal serum in capillary, or other small tubes. The test
is regarded as positive if there is precipitation at the interface (Fox, 1998).
When the antigens are layered over the antibodies, the antigens are precipitated out of solution by the antiserum when antigen and antibodies are related.
Latex-Agglutination Test
In this test, the antibodies are first adsorbed onto the much larger latex
particles and mixed with the antigen preparation (sap of diseased tissues).
An opalescent suspension containing antigen particles will settle out, producing a clear supernatant by granulation or flocculation in a positive test.
Ouchterlony Immunodiffusion Test
In this test, a petri dish containing 1 percent agar is used. A central well
(usually 0.5 cm in diameter), surrounded by several satellite wells, is cut
into the agar. The antibody is placed on the central well, whereas the antigen
is placed on surrounding wells. The antibodies and antigens are allowed to
approach each other by diffusion in agar. In the positive test, a precipitin
band, usually visible to the naked eye, forms at the leading edge where diffusing antigen and antibody molecules meet.
Immunoelectrophoresis
By this method, mixtures of antigens are separated before immunodiffusion. A narrow trough is cut in a layer of thin gel parallel to an electric
current that passes close to the antigens along the length of the gel. Each antigen moves in a separate wave at a characteristic rate according to its distinct charge. As a result, proteins separate into bands. Once the proteins have
separated sufficiently, the current is switched off and antiserum is added to
the trough cut in the gel. Precipitin arcs composed of complexes of antibodies and antigens form where the individual electrophoresced antigens have
reached.
Antigen-Capture Indirect ELISA
Infected plant sap is used as an antigen. Many variations of ELISA exist,
such as antigen-capture indirect ELISA, direct sandwich ELISA, double antibody sandwich ELISA (DAS-ELISA), triple antibody sandwich ELISA,
and F(ab')2-based ELISA.
In the antigen-capture indirect ELISA method, the wells are coated with
the antigen. The remaining sites are blocked by a blocking agent, and a specific primary antibody is added. A secondary antibody-enzyme conjugate
binds to the primary antibody. This is followed by the addition of an enzyme
substrate leading to a colored product in proportion to the concentration of
pathogen. This methodology involves coating immunoplates with the antigen and then centrifuging them at 600 g for 5 min at 4C. The plates are postfixed by incubation of 100 L per well of 0.25 percent glutraldehyde solution for 5 to 7 min at room temperature. The fluid is flicked off by inverting
the plate, and the plates are washed three times with either tris-buffered saline (TBS) Tween (TBST) or phosphate-buffered saline (PBS) Tween (PBST)
followed by a short period of drying by laying the plates on a paper tissue.
About 200 g blocking solution (2 percent casein in either TBST or PBST)
is absorbed to the remaining sites in the well for 1 h at room temperature.
After one washing, the antibody in TBST or PBST is added at 50-100 L
per well. After 1 to 2 h incubation at 37C and three further washings,
antimouse immunoglobulin IgG alkaline phosphatase conjugate diluted in
TBST or PBST containing casein (2 percent) is incubated for 1 to 2 h at
37C (50-100 L per well). The excess fluid is removed by flicking the
plate, followed by washing with TBST or PBST for three to five times.
Phosphatase tablets are freshly dissolved in diethanolamine (DEA) buffer,
pH 9.6, and this substrate solution is pipetted into the wells. Substrate conversion is quantified by measuring absorbance at 405 nm with a plate reader
(after at least 30 min incubation in the dark at 37C).
Direct Sandwich ELISA

In the direct sandwich ELISA method, 96-well immunoplates are coated
with the specific antibody (polyclonal or preferably monoclonal antibody)
and incubated successively with the antigen containing sample followed by
a second enzyme-labeled specific antibody that is directly conjugated with
an enzyme. This leads to a colored product in proportion to concentration of
pathogen.
Double Antibody Sandwich ELISA
In the double antibody sandwich ELISA method, a specific capture antibody is immobilized onto a solid surface (Huttinga, 1996), such as the wells
of a microtiter plate. The infected plant tissue sample is added, and unbound
material is washed away. Bound antigen is detected by the addition of a detecting antibody that has been conjugated with an enzyme, and unbound
material is again washed away. The presence of the detecting antibody is determined through the addition of a substrate for the enzyme. The amount of
color that develops is proportional to the amount of antigen present in the
sample. The intensity of the color can be recorded by automated equipment
(Miller, 1996).
Triple Antibody Sandwich ELISA
In the triple antibody sandwich ELISA method, a specific antibody produced in one animal species is bound to the solid substrate (96-well plates),
while a second specific antibody produced in another animal species binds
to the bound antigen. The sandwich is detected by an antibody-enzyme
conjugate that binds to the second antibody (Miller, 1996).
F(ab')2-Based ELISA
In F(ab')2-based ELISA, F(ab')2 fragments from target-specific antibodies are used as the capture reagent. A specific second antibody produced in
the same animal species binds to antigen already bound to the F(ab')2 fragments. The sandwich in turn is detected by the addition of a general detecting antibody-enzyme conjugate that reacts specifically with the Fc portion
of the second antibody (Miller, 1996).
Dipstick ELISA
ELISAs can be carried out on membranes made of nitrocellulose, nylon,
or other materials. In the dipstick assay, nitrocellulose dipsticks surfacecoated with a capture antibody are used. Dipsticks on which specific MCAs
have been bound are dipped into the test specimen, allowing ELISA reactions to occur in situ. Flexible plastic dipsticks are quick and easy to use in
the field to test sap squeezed onto the coated surface. Any pathogen (particularly viruses) present is entangled and detected following incubation with
the antibody-enzyme conjugate, washing, and then the substrate, further
washing, and finally the stopping solution. If the specified pathogen is present, the dipstick becomes colored, whereas if free of pathogen it remains
colorless.
Dot-Blot ELISA
In this assay system, ELISA reactions are carried out on nitrocellulose
membranes. A drop containing the specific monoclonal antibody is absorbed as a dot, onto which a drop of the test sample is later added and
blotted (Fox, 1998).
Direct Tissue Blot Immunoassay
This assay is also called tissue print ELISA, immunoprinting, or direct
tissue print immunoassay. In this assay, stems and leaf petioles are cut with a
razor blade, and the cut surface is pressed gently and evenly to the nitrocellulose membrane. These blots are allowed to dry for 10 to 30 min, incubated with the monoclonal antibodies for 2 h, and rinsed with PBST buffer
for 10 min. The blots are labeled with an enzyme conjugate, goat antimouse
Ig (H + L), for 1 to 2 h at 37C, rinsed with PBST-polyvinylpyrrolidone
(PVP) buffer, and rinsed again with TTBS (20mM tris (hydroxymethyl)
aminomethane, 500 mM sodium chloride, and 0.05 percent Tween 20), each
for 10 min. The blots are incubated with freshly prepared NBT-BCIP (nitroblue tetrazolium, 5-bromo-4-chloro-3-indolyl phosphate in sodium carbonate buffer) substrate for 5 to 20 min. After stopping the reaction by putting
the blots in water in a petri dish, the blots are observed under a light microscope. Development of an area with an intense purple color located at
phloem tissue cells is considered a positive reaction (Lin et al., 2000).
In Situ Immunoassay (ISIA)

In this assay, sections 100 to 200 M thick are cut from stems, petioles, or
veins of diseased plants. The sections are transferred to 24-well plastic
plates and fixed with 70 percent ethanol for 5 to 10 min. After the alcohol is
removed using a pipette, the sections are incubated with specific monoclonal antibodies for about 30 min. The sections are washed with PBSTPVP for 5 to 10 min, and incubated with enzyme-labeled secondary antibodies (alkaline phosphatase conjugated goat antirabbit IgG) at 37C for 30
to 60 min. The sections are washed again with PBST-PVP and with TTBS
buffer. Sections are then incubated with freshly prepared NBT-BCIP substrate mixture for 5 to 15 min. After stopping the reaction by removing the
substrate solution, the sections are transferred to a glass slide and observed
under a light microscope. The development of a purple color in the phloem
tissue cells is considered as a positive reaction (Lin et al., 2000).
Recombinant Enzyme-Linked Immunosorbent Assay
In this assay, monoclonal antibodies are subcloned from hybridoma cell
lines and expressed in Escherichia coli as single-chain variable fragment
antibody (scFv) fusion proteins. The scFv technology allows the cloning of
variable genes from preexisting monoclonal antibodies cell lines, linking
them with a flexible peptide as an scFv. These constructs are expressed in
bacteria as soluble proteins or fused with the capsid proteins of filamentous
bacteriophages. In addition, antibody genes are expressed fused with other
proteins such as alkaline phosphatase or with amphipathic helices (Terrada
et al., 2000).
Phage Display Technology
Recombinant antibody technology, in combination with phage display
technology, provides a useful approach for the production of target-specific
antibodies without the use of laboratory animals and time-consuming immunization protocols (Griep et al., 2000). Recombinant antibodies are
raised from a human combinatorial antibody library. Helper phages, which
contain the entire phage genome but lack an efficient packaging signal, are
used to rescue phagemids from the combinatorial library. When both the
helper phage and single-chain variable fragment encoding the phagemid
vector are present within the same bacterium (e.g., Escherichia coli), phages
are assembled that carry scFv-antibodies (phage antibodies [PhAbs]) on
their surface and contain the scFv-encoding phagemid vector. Consequently,
within PhAbs, the genotype and phenotype are linked. To select for antigen
specificity, PhAbs rescued from the combinatorial antibody library are allowed to bind to immobilized target antigens (panning). Washing removes
PhAbs that lack affinity for the target antigens. Bound PhAbs are eluted, and
selected PhAbs are applied to three sequential rounds of panning to further
enhance the percentage of target-reactive PhAbs (Griep et al., 2000). PhAbs
are selected to major structural proteins of the test virus. The scFv-encoding
genes are retrieved and expressed in E. coli as ready to use antibody-enzyme
fusion proteins. After subcloning, the encoding DNA sequences in the expression vector pSKAP/S, which allowed the scFvs to be expressed as alkaline phosphatase fusion proteins. The antibodies are used as coating and detecting reagents in a DAS-ELISA (Griep et al., 2000).
Latex-Protein A Agglutination Assay
In this assay, inert particles such as latex beads are used to detect antigen/antibody complexes. Protein A, a cell surface protein from Staphylococcus aureus that binds nonspecifically to the heavy chains of mouse and
rabbit IgG antibodies, is used to link the particles coated with the specific
antibodies by shaking the two together. Samples of plant extracts are tested
by incubation with coated particles. If the test pathogen is present in the
sample, the antigens form bridges linking the particles together, resulting in
agglutination.
Immunofluorescence
Two methods of immunofluorescence are used to diagnose plant diseases. In the direct immunofluorescence method, specific antibodies bound
to their target antigens are detected by using second antibodies conjugated
with fluorescent dyes such as fluorescein isothiocyanate (FITC) or rhodamine isothiocyanate. Fluorescence, indicating the presence of the target antigen, is visualized microscopically. The microscope should have a special
device for fluorescence using ultraviolet light (fluorescence microscopy). In
direct immunofluorescence, the fluorescent dye is conjugated directly to the
specific antibody (Salinas and Schots, 1994). Usual protocols involve extraction of the pathogen fraction from the tissue of individual or composite
plants that is fixed to a well of a multiwell glass microscope slide by flaming
or acetone treatment. Fixed preparations are stained directly by conjugated
antibody or by the indirect procedure in which the primary antibody is not
conjugated but a secondary conjugated antibody is bound to the primary one
(De Boer et al., 1996).
Immunosorbent Electron Microscopy

This assay system is mostly used for the diagnosis of virus diseases.
Electron microscope grids coated with carbon strongly adsorb protein, and
when they are floated on a drop of antiserum containing antibodies to the
pathogen, the antibodies become attached. The grids are then floated on a
drop of the sap of an infected plant. After staining, the pathogen (particularly virus particles) adsorbed to the antibodies can be seen under a transmission electron microscope.
Immunosorbent Dilution-Plating Technique
In this method, bacterial pathogens are trapped on antibody-coated petri
dishes or inoculation rods so they can be incubated on selective media. Any
unbound bacteria are removed by washing. The colonies that form are detected by drying the agar and staining by immunofluorescence.
Western Blotting
This technique involves the transfer of proteins or glycoproteins on
polyacrylamide gels electrophoretically onto a membrane or solid phase
and the probing of such membrane-bound antigens with a specific antibody
solution, based on the covalent binding of the antibody and its antigen (Fox,
1998). In the electrophoresis, different proteins move apart to form distinct
bands. The larger molecules migrate more slowly than the smaller molecules. The protein in these bands can be transferred by blotting to a strip of
porous nitrocellulose material. Afterward, specific polyclonal or monoclonal antibodies are used to bathe nitrocellulose for about an hour. An
enzyme/substrate system (phosphatase or peroxidase conjugate), similar to
an ELISA reaction, is also used to locate the position of bound antibodies
(Fox, 1998).
Immunogold Assay
In the immunogold assay, following the electrophoresis of proteins on
SDS polyacrylamide gel, proteins are transferred to a nitrocellulose membrane. Monoclonal antibody specific to a protein of the test pathogen is conjugated to gold particles and applied on the nitrocellulose membrane. The
signal appears as a band and can be visualized on the membrane directly.
Radioimmunoassay
In the radioimmunoassay method, the protein is transferred to a nitrocellulose filter after electrophoresis, and a monoclonal antibody is added to
it. MCA binds to a specific protein, and a radioactive detector antibody is
added. The band is visualized indirectly by preparing an autoradiograph.
NUCLEIC ACID PROBE-BASED METHODS
Both DNA and RNA probes are used for crop disease diagnosis. DNA is
double-stranded and the two strands are held together via specific hydrogen
bondings between the base pairs. Pairing occurs with great specificity: Adenine (A) pairs only with thymine (T), and guanine (G) only with cytosine
(C). The interaction of the two single-stranded molecules is known as hybridization. Several physical conditions can denature the DNA into single
strands. High temperature and strongly alkaline pH denature double-stranded
DNA. By manipulating the physical conditions, a strand can anneal with another strand of the target DNA. Nucleic acid hybridization, or reassociation,
is a process by which complementary single-stranded nucleic acid anneals
to double-stranded nucleic acid. This process occurs because of the hydrogen bonds between the two strands of the DNA. The pairing can also take
place between DNA and RNA or RNA and RNA, and the pairing can occur
between adenine (A) and uracil (U) in RNA instead of thymine (T) in DNA
and guanine (G) and cytosine (C). Single-stranded DNA and RNA can act as
probes. A diagnostic nucleic acid probe may be defined as a nucleotide sequence, labeled with a reporter molecule, that is able to identify a target
pathogen within a test sample by selectively hybridizing to the complementary sequence present within a microorganisms DNA (De Boer et al., 1996).
Nucleic acid probes are sequences of nucleic acids that are labeled with a
marker and used to detect complementary nucleic acid sequences in a sample. Probes, which can be either DNA or RNA, range in size from 15 to several thousand base pairs (bp). Hybridization protocols usually require the
probe or target sequence to be immobilized on a solid surface, but the reaction can also occur in solution or in situ. The probe-target hybrids are visualized using autoradiography, colorimetric assays, or chemiluminescence.
Hybridization Formats
Nucleic acid hybridizations may be performed in solution or on a solid
support. In a solid-support format, the target nucleic acid from the pathogen
to be detected is immobilized on a nitrocellulose or nylon membrane. DNA
is first cut into fragments by a restriction enzyme, often followed by gel

electrophoresis and denaturation (Southern, 1975). RNA is usually transferred by a similar technique known as Northern blotting. After it has been
blotted onto the support, the target nucleic acid is fixed by baking or crosslinking under UV light. The labeled probe hybridizes to the target DNA. After the excess unbound probe has been removed by washing, the hybrid is
detected by a suitable assay (Fox, 1998). The diagnostic probes are labeled
with radioisotopes, primarily 32P. Nonradioactive methods are also available for labeling DNA probes. Nonradioactive probes may be labeled enzymatically or chemically. Biotin, a vitamin, is commonly used for labeling,
and the modified nucleotide is biotin-11-dUTP. Probes attached to biotin
bind to avidin (a protein) extremely tightly. As a result, a more effective
complex of many enzyme molecules can be produced by binding enzymes
first to biotin and then to avidin, around which they cluster (Fox, 1998). The
steroid digoxigenin may be employed in a similar way with antidigoxigenin
enzymes for labeling. Sulfonation of cytosine residues by sulfite was one of
the first hapten-based labeling systems (De Boer et al., 1996). The probe is
detected using a monoclonal antisulfonate antibody followed by an alkaline
phosphatase-conjugated antimouse IgG antibody. Another method of chemical labeling involves photobiotin, a compound in which biotin is bound to
aryl azide by a linker arm. In the presence of light, aryl azide is converted
into an aryl nitrene moiety that reacts with nucleic acid. Oligonucleotides
are most efficiently labeled chemically by a reactive amine. A detectable
group, such as alkaline phosphatase or horseradish peroxidase, is added to
the amino-oligonucleotide after synthesis (De Boer et al., 1996).
Many commercial nucleic acid-based tests employ the sandwich assay,
in which adjacent sections of the target nucleic acid are hybridized by two
probes. One is the capture probe, which by hybridization links the target to
the solid support to which this probe is already bound. The other probe is labeled with a reporter group, which may be a fluorescent molecule, an enzyme, or a radioactive atom that hybridizes with an adjacent section of the
target nucleic acid. The reporter group with a fluorescent molecule can be
readily distinguished, even in minute amounts (Fox, 1998).
Dot-Blot Diagnosis
When an extract or cell sap believed to contain the target nucleic acid is
dotted on a filter, the method is called dot-blot. Dot-blot is a routine spot hybridization method. In this method, the DNA is not cut by restriction enzymes and fractioned. The DNA is applied as a small drop of sap extracted
from an infected plant directly onto a nitrocellulose sheet. The DNA is then
dried and hybridized with the probe in a sealed plastic bag using a buffer extract (Maule et al., 1983). The nucleic acid is loaded into a multisample vacuum manifold, which is used to spot it onto the membrane.
Colony Blot
Colony blot can be useful to detect bacteria in infected tissues. Dilutions
of the bacteria from an infected tissue are grown on nitrocellulose filters
placed on suitable agar media. After incubation, the nucleic acids are simultaneously released and denatured with alkali or by incubation in a microwave oven. The filter is then washed and baked prior to hybridization as a
colony.
In Situ Hybridization Assay
In this assay, host tissue sections are fixed to a microscope slide and exposed to probe DNA. In another type of assay, sections of cut plant tissue are
pressed against the nitrocellulose filter, which is then treated with alkali to
denature the DNA and the tissue print is fixed on the filter. Hybridization reveals the presence of the pathogen.
PCR-Based Methodology
The polymerase chain reaction provides a powerful and rapid technique
to exponentially amplify specific DNA sequences by in vitro DNA synthesis (Henson and French, 1993). Three essential steps to a PCR include
(1) melting the target DNA, (2) annealing two oligonucleotide primers to
the denatured DNA strands, and (3) extending the primer via a thermostable
DNA polymerase. Newly synthesized DNA strands serve as targets for subsequent DNA synthesis since the three steps are repeated up to 50 times. The
specificity of the method derives from the synthetic oligonucleotide primers, which base-pair to and define each end of the target sequence to be amplified (Henson and French, 1993).
PCR uses a thermostable Thermus aquaticus (Taq) DNA polymerase to
synthesize DNA from oligonucleotide primers and template DNA. The template DNA may be genomic, first-strand cDNA, or cloned sequences.
Primers are designed to anneal to complementary strands of the template
such that DNA synthesis initiated at each primer results in replication of the
template region between the primers.
The PCR involves three distinct steps governed by temperature. DNA,
primers, deoxynucleotides, buffer, and Taq polymerase are combined in a
microcentrifuge and overlaid with mineral oil. The tube is placed in a

thermocycler programmed to repeat a set of short incubations at predetermined temperatures. In the first step, the template DNA is denatured to separate the complementary strands. This is done at 95C for 5 minutes. In the
second step, the mixture is held at an annealing temperature to allow the
primers to hybridize to their complementary sequences. This is done at 55C
for 1 min. A PCR primer may comprise two regions, a 3' (priming) region
and a 5' (variable) region. The most important region in determining the efficiency of annealing and subsequent DNA synthesis during the PCR is the 3'
region, which should be perfectly complementary to the template sequence.
The priming region should normally be 20 to 25 bases long. The Taq polymerase stabilizes these base-paired structures and initiates DNA synthesis.
In the last step, the reaction is heated to about 72C for 1 to 5 minutes. This
process leads to a Taq polymerase-directed DNA synthesis. The cycle is repeated by keeping the reaction tubes in a thermal cycler for more than 20
times. In the first cycle each template gives rise to a newly synthesized complement. Thus, the number of copies of the target region is doubled. Similarly, in each subsequent cycle, the DNA concentration corresponding to the
target region is almost doubled. About 20 cycles of PCR would produce
106-fold amplification of the target DNA.
Initially, very low numbers of DNA molecules can be multiplied enormously by PCR. Only a few nanograms of the initial template DNA is necessary for amplification. Both dsDNA and ssDNA can be amplified by PCR.
It is possible to amplify RNA by reverse transcription (RT) into a cDNA
copy by RT-PCR. Synthetic oligonucleotides (primers) that are complementary to the end sequences can be produced. Because the PCR process does
not depend on the use of purified DNA, the host tissue extract can also been
used for diagnosis.
The PCR product is analyzed by agarose gel electrophoresis. The PCR
product from a defined band can be recovered from agarose gel. The DNA
generated in a PCR can be reamplified and used for sequencing. DNA sequencing reactions are performed using commercially available kits of T7
polymerase or Sequenase.
RAPD (random amplified polymorphic DNA) PCR is an important diagnostic tool. Oligonucleotides of arbitrary sequence are used as primers for
amplification. Shorter sequences on the order of 100 to 1,000 base pairs are
most efficiently amplified and easily resolved by agarose electrophoresis.
Hence, DNA sequences within a few hundred base pairs are usually chosen
as primer annealing sites. In these cases, pairs of sequences complementary
to the primer may be close to one another and arranged with 3' ends pointing
toward one another. Under these circumstances, annealing of the primer to
the target genome will result in the production of an amplified fragment af-
ter appropriate thermal cycling. RAPD PCR with DNA of infected plant tissues is performed as follows: In a microcentrifuge tube, genomic DNA (25
to 50 ng), deoxynucleoside triphosphate (dNTP) (200 m), primer (200
pmol), PCR buffer (100 mM Tris-HCl [pH 8.3], 500 mM KCl, 15 mM
MgCl2, 1 mg/mL gelatin, 0.1 percent Tween-20, 0.1 percent NP), and 1 unit
Taq polymerase are mixed, spun briefly, and overlaid with 50 L mineral
oil. Forty-five PCR cycles are performed at 94C for 1 min, 35C for 1 min,
and 72C for 2 min. The reaction mixture (10 L) is run through a 1 percent
agarose gel in tris borate ethylene diamine tetra-acetic acid (TBE) buffer
(89mM Tris, 89 mM boric acid (pH 8.3), 2.5 mM EDTA [ethylene diamine
tetra-acetic acid]).
Any DNA or RNA sequence that is specific for a particular organism can
be used for detection of that organism. Potential targets specific to pathogens include specific sequences from mitochondrial DNA fragment, pathogen-specific plasmid sequences, and short, interspersed repetitive elements
present in bacteria. Ribosomal genes and the spacers between them possess
conserved as well as variable sequences, and can be amplified and sequenced with universal primers based on their conserved sequences. Genes
for 5.8S, 18S, and 26S (28S) fungal nuclear ribosomal RNA genes (rDNA)
are used as primers (Henson and French, 1993).
Considerably greater sequence variation is found in the internal transcribed spacer (ITS) regions between the rRNA genes within an rRNA repeat unit (rDNA). Even more sequence differences are in the nontranscribed
spacer (NTS) regions between the rDNA repeat units, and still more are in
the intergenic spacer regions or noncoding sequences that occur within the
rDNA repeat unit of some fungi (Henson and French, 1993). Any organism
that has rDNA repeats may be specifically detected by selecting primer sequences based on variable spacer regions.
Immuno-PCR and Immunocapture PCR
Disease diagnosis methods that combine antibody binding and PCR are
highly sensitive and can detect microbial antigens in addition to their nucleic acids. In the immuno-PCR method, a DNA fragment is molecularly
linked to antigen-antibody complexes. Protein A and streptavidin portions
of the linker molecule bind the antibody and DNA, respectively. Antigen
present in a sample binds the specific antibody, which, in turn, binds the
linker molecule. The latter is bound to a nonspecific, biotin-labeled DNA
sequence that is subsequently amplified by PCR. Immuno-PCR is 105 times
as sensitive as the enzyme-linked immunosorbent assay for the detection of
antigen (Sano et al., 1992).
Immunocapture PCR is another sensitive diagnostic assay. Whereas immunoPCR requires the antigen-specific antibody only, immunocapture PCR requires the antigen-specific antibody and nucleic acid sequence information
from the microbe being detected.
Immunocapture-Reverse Transcription-Polymerase Chain Reaction
(IC-RT-PCR)
In a typical IC-RT-PCR assay, PCR tubes are rinsed in PBST, soaked in
ethanol for 15 min, and air dried. The tubes are coated with the specific virus
antiserum in a coating buffer (15 mM Na2CO3; 35 mM NaHCO3; 3 mM
NaN3; pH 9.6) for 3 h at 37C. The tubes are washed three times with PBST
and blotted dry on a tissue. They are placed at 70C for at least 10 min and
the contents thawed at 94oC for 2 min to disassemble the antibody-bound virus and free the viral RNA from the protein coat. A total of 50 L of RTPCR mix (4 L of 5 first-strand reverse transcription buffer; 2 L of DTT;
2.6 L of dNTP; 0.1 L of RNasin RNase inhibitor; 0.25 L of SuperScript
RNase H- Reverse Transcriptase; 3 L of 10 PCR buffer; 4.2 L of 25 mM
MgCl2; 1 L of reverse primer; 0.5 L of forward primer; 0.2 L of Taq
polymerase; and 32.75 L of nuclease-free water) as added to each tube.
The tubes are then treated as follows: 37C for 1 h, 94C for 2 min (35 cycles
of 94C for 30 s, 50C for 30 s, and 72C for 60 s), and 72C for 10 min. Following RT-PCR, the products are assessed by electrophoresis in 1.5 percent
agarose gels and stained with ethidium bromide (Gillaspie et al., 2000).
Repetitive-Sequence-Based Polymerase Chain Reaction (Rep-PCR)
Rep-PCR is based on PCR-mediated amplification of DNA sequences
located between specific interspersed repeated sequences in microbial genomes. These repeated elements are termed BOX, REP, and ERIC elements.
Amplification of the DNA sequences between primers based on these repeated elements generates an array of different-sized DNA fragments from
the genomes of individual strains. The separation of these fragments on
agarose gels yields highly specific DNA fingerprints that can be either visually compared or subjected to computer-assisted pattern analysis (McDonald et al., 2000). The rep-PCR is useful in the identification of bacterial
pathogens and disease diagnosis (Louws et al., 1999). Some fungal pathogens can also be identified by this technique (Jedryczka et al., 1999).
DIAGNOSIS OF CROP FUNGAL DISEASES

Several fungal pathogens survive in vegetative propagating materials and
may serve as the primary inoculum source for the spread of the disease.
Early diagnosis of the inoculum may help in eradicating the disease in the
field. As many fungicides are specific only to certain pathogens or groups of
pathogens, early diagnosis will be useful in the selection of the most appropriate treatment. Current detection of pathogenic fungi is based on visual inspection of characteristic symptoms. When sporulation is present, general
confirmation can be made by light microscopy (Miller, 1996). The pathogen
may be isolated by transferring diseased plant tissue to an agar medium in
petri dishes. Sometimes a selective medium may have to be used. A bioassay is required to test the pathogenicity of isolates, particularly when
closely related saprophytic species are common.
Several immunoassays have been developed to diagnose fungal diseases.
Immunoassay kits are commercially available to detect species of Rhizoctonia, Phytophthora, and Pythium and Septoria nodorum (= Stagonospora
nodorum), S. tritici, and Pseudocercosporella herpotrichoides (Miller, 1996).
Tilletia controversa in wheat, Phytophthora fragariae in strawberry, and
Rhizoctonia spp. in poinsettia are detected by DAS-ELISA (Miller, 1996).
Indirect ELISA assays are used to detect Spongospora subterranean in potato (Harrison et al., 1994), Colletotrichum acutatum and Phytophthora spp.
in strawberry, Leptosphaeria maculans in canola, and Fusarium oxysporum
f. sp. narcissi in narcissus (Miller, 1996). Verticillium dahliae in potato is
detected by immunofluorescence assays (Miller, 1996).
Gaeumannomyces graminis in wheat can be detected by Southern blot
with cloned mitochondrial DNA labeled with 32P or digoxigenin as a probe
(Bateman et al., 1992). Phytophthora capsici, P. cinnamomi, and P. palmivora can be detected by PCR and dot-blot with an rDNA ITS (internal transcribed spacer) probe, labeled with 32P (Lee, White, et al., 1993). Phytophthora parasitica and P. citrophthora can be detected by dot-blot with cloned
chromosomal DNA, as a probe labeled with 32P (Goodwin, English, et al.,
1990; Goodwin, Kirkpatrick, et al., 1990). Southern blot with cloned chromosomal DNA as a probe, labeled with biotin, is useful in detecting P. infestans (Moller et al., 1993).
Fusarium culmorum can be detected by Southern blot with cloned
genomic DNA as a probe, labeled with digoxigenin (Koopmann et al.,
1994). Leptosphaeria maculans in rapeseed can be detected using RAPDPCR with amplified chromosomal DNA as a probe, labeled with digoxigenin (Schafer and Wostemeyer, 1994). Dot-blot with cloned total DNA as a
probe, labeled with 32P is used to detect Pyrenophora teres and Pyrenophora graminea (Husted, 1994). Slot-blot with cloned genomic DNA as a
probe, labeled with 32P will be useful to detect Pseudocercosporella herpotrichoides in cereals (Nicholson and Rezanoor, 1994). Pythium ultimum can
be detected by PCR and dot-blot using rDNA ITS as a probe, labeled with
digoxigenin (Levesque et al., 1994).
Botrytis cinerea infection in pear stems can be detected by plating stem
halves on a selective medium and by ELISA, which is a more sensitive
method (Meyer et al., 2000). The PCR assay is highly sensitive and reproducible as a tool for the detection and identification of fungi when speciesspecific primers are carefully selected. Several fungal pathogens have been
detected using this method, including Verticillium spp., Fusarium spp.,
Rhizoctonia oryzae, Gaeumannomyces graminis, Magnaporthe poae, Leptosphaeria korrae, and Phiolophora gregata (Fouly and Wilkinson, 2000).
Nuclear rDNA of fungi consists of small and large subunits, a 5.8S region, and an internal transcribed spacer region(s). Each subunit and region
base sequence is variable among the genera and species of fungi. This variability can be used to detect fungal pathogens. For example, the ITS region
of Gaeumannomyces is highly variable among its species and less variable
among its varieties. However, the small subunit of nuclear rDNA is distinctly variable among the varieties of G. graminis. This small subunit of
nuclear rDNA (18S rDNA) is used for the detection of G. graminis varieties
using PCR amplification (Fouly and Wilkinson, 2000). A PCR-based diagnostic assay has been developed to detect Rhynchosporium secalis in barley
(Lee et al., 2001). RAPD analysis is useful to characterize P. infestans isolates from potato and tomato (Mahuku et al., 2000). Species-specific primers were designed based on sequence data of a region consisting of the 5.8S
RNA gene and internal transcribed spacers 1 and 2 of R. secalis (Lee et al.,
2001). An oligonucleotide primer set, RS8 and RS9, was used in detecting
R. secalis. This primer amplified a 264 bp fragment from the DNA of all R.
secalis isolates. This is used to detect R. secalis in infected barley tissues
(Lee et al., 2001). A species-specific PCR has been developed to detect
black sigatoka and yellow sigatoka leaf-spot pathogens in banana (Johanson
et al., 2000). The Rep-PCR technique is used to detect species of Fusarium,
Stagonospora, Septoria, Tilletia, and Leptosphaeria (Jedryczka et al., 1999;
McDonald et al., 2000).
DIAGNOSIS OF CROP BACTERIAL DISEASES
Several diagnostic techniques have been developed to diagnose fire
blight of apple and pear. The available methods include isolation of the
pathogen on the semiselective media of Miller and Schroth, Crosse and
Goodman agar, or crystal violet-cycloheximide-thallium nitrate (CCT) agar
(Merighi et al., 2000). A minimal medium 2-copper sulfate agar was developed to specifically identify Erwinia amylovora (Bereswill et al., 1998).
Isolation followed by pathogenicity tests will also be useful to detect the
pathogen. Miller (1983) described an immunofluorescent microscopic method
for the detection of E. amylovora. The double-antibody sandwich indirect
enzyme-linked immunosorbent assay (DASI-ELISA) was developed to detect the pathogen (Gorris et al., 1996). An analysis of fatty acid methyl esters by gas chromatography (GC-FAME) is also used to detect E. amylovora
(van der Zwet and Wells, 1993). E. amylovora can be detected by nested
PCR, PCR dot-blot, and reverse-blot hybridization methods (McManus and
Jones, 1995). PCR techniques are extensively used to detect the bacterium
(Bereswill et al., 1995; Guilford et al. 1996). A PCR-ELISA was developed
by Merighi et al. (2000) to detect the pathogen. A PCR-based method detected E. amylovora effectively in pear (Sobiczewski et al., 1999). A RepPCR technique is used to identify several bacterial pathogens (Louws et al.,
1999).
An immunofluorescence test is used to detect Clavibacter michiganensis
subsp. michiganensis in tomato, C. michiganensis subsp. sepedonicus in potato, and Erwinia chrysanthemi in carnation (De Boer et al., 1996). ELISA
is useful in detecting C. michiganensis subsp. michiganensis in tomato, C.
michiganensis subsp. sepedonicus in potato, E. chrysanthemi in carnation,
Pantoea stewartii in carnation, Xanthomonas campestris pv. campestris in
cabbage, and Pseudomonas savastanoi pv. phaseolicola in bean (De Boer et
al., 1996). X. translucens pv. undulosa in wheat and X. vesicatoria in pepper
and tomato are detected by dot immunoassay (De Boer et al., 1996).
Nucleic acid probes have been employed to detect some bacterial pathogens. A dot-blot assay has been employed to detect Erwinia carotovora,
Xanthomonas axonopodis pv. citri, X. axonopodis pv. phaseoli, Pseudomonas syringae pv. tomato, and Clavibacter michiganensis subsp. michiganensis. Colony blot assay is used to detect Agrobacterium tumefaciens, E.
amylovora, Pseudomonas savastanoi pv. phaseolicola, and P. syringae pv.
morsprunorum (De Boer et al., 1996). PCR is used to detect A. tumefaciens
(Dong et al., 1992), Clavibacter michiganensis subsp. sepedonicus (Schneider et al., 1993), Ralstonia solanacearum (Seal et al., 1992), and X. axonopodis pv. citri (Hartung et al., 1993). PCR and RFLP-based techniques are
useful in detection of A. vitis in grape (Burr and Otten, 1999).
DIAGNOSIS OF CROP VIRAL DISEASES
A number of different serological techniques have been developed for
detecting Citrus tristeza virus (CTV). These include ELISA (Rocha-Pena
and Lee, 1991), sodium dodecyl sulfate (SDS)-immunodiffusion (Gransey

et al., 1978), immunoelectron microscopy, radioimmunosorbent assay (RochaPena and Lee, 1991), immunogold assay, Western blot assay (Rocha-Pena
and Lee, 1991), dot immunobinding assay (Rocha-Pena et al., 1991), direct
tissue blot immunoassay (Lin et al., 2000), and in situ immunofluorescence
(ISIF) (Brlansky et al., 1988). Specific monoclonal antibodies produced by
hybridoma technology are used for ELISA. In spite of the high serological
variability of CTV, a mixture of two monoclonal antibodies (3DFI and
3CA5) is able to detect all CTV isolates tested. These antibodies were patented in 1984 and are considered an international reference for CTV diagnosis. With these commercially available monoclonal antibodies, approximately 2 million samples have been analyzed since 1990 (Terrada et al.,
2000). Lin et al. (2000) developed an in situ immunobioassay that does not
use fluorescent dyes. This assay is a simple and specific procedure that detects CTV in infected citrus plants in about 2 h.
Terrada et al. (2000) obtained single-chain variable fragment antibodies
that bind specifically to CTV from the hybridoma cell lines 3DF1 and
3CA5. These scFv were genetically fused with dimerization domains as
well as with alkaline phosphatase, and diagnostic reagents were produced
by expressing these fusion proteins in E. coli cultures. The engineered antibodies were successfully used for CTV diagnosis in citrus plants by tissue
print ELISA and DAS-ELISA. The fully recombinant ELISAs were as specific and as sensitive as conventional ELISAs performed with the parental
monoclonal antibodies (Terrada et al., 2000).
ELISA and Western blots using monoclonal antibodies are recommended
to identify grapevine leafroll-associated viruses. The monoclonal antibodies of these viruses are commercially available (Monis, 2000). High-quality
polyclonal antisera have been raised against several members of the genus
Tospovirus including Tomato spotted wilt virus, Groundnut ringspot virus,
Tomato chlorotic spot virus, and Watermelon silverleaf mottle virus, and
Impatiens necrotic spot virus. These antisera are now widely applied for
Tospovirus detection with the aid of a DAS-ELISA (Griep et al., 2000).
Polyclonal antisera, although widely used in routine diagnosis, are available
in limited amounts, and their specificity varies from batch to batch. The antisera are being replaced by monoclonal antibodies, which can be produced
indefinitely. Several monoclonal antibodies against tospoviruses have been
developed. Griep et al. (2000) used recombinant antibody technology in
combination with phage display technology to produce Tomato spotted wilt
virus-specific antibodies. A panel of recombinant single-chain variable antibodies against the N protein and G1 and G2 glycoproteins of TSWV was
retrieved from a human combinatorial scFv antibody library using the phage
display technique. Antibodies were obtained after subcloning the encoding
DNA sequences in the expression vector pSKAP/S, which allowed the

scFvs to be expressed as alkaline phosphatase fusion proteins. An antibody,
N56-AP/S, at a concentration of 0.1 g/ml, can detect as little as 1 ng of N
protein of TSWV in a DAS-ELISA. The CL (mouse light-chain) ZIP
(leucine zipper) fusion protein of scFv N56 was an effective coating and detecting reagent in a DAS-ELISA or detection of TSWV (Griep et al., 2000).
ELISA is commonly used to detect Peanut stripe virus and Peanut mottle
virus in peanut seed and vegetative tissues. Gillaspie et al. (2000) developed
an immunocapture reverse-transcription polymerase chain reaction (IC-RTPCR) for detection of peanut virus diseases. Peanut tissue slices were extracted in a buffer and centrifuged, and a portion of the supernatant was incubated in a tube that had been coated with an antiserum to either PStV or
PeMV. Following immunocapture of the virus, the tube was washed and the
RT-PCR mix (with primers designed from conserved sequences within the
capsid region of each virus) was placed in the same tubes. The IC-RT-PCR
method was more sensitive than ELISA in the detection of peanut viruses
(Gillaspie et al., 2000). Prunus necrotic ringspot virus, Prune dwarf virus,
and Apple mosaic virus affect stone-fruit (plum, almond, apricot, cherry,
and peach) trees. Nonisotopic molecular hybridization and multiplex reverse-transcription polymerase chain reaction methodologies have been developed to detect all these viruses simultaneously (Saade et al., 2000). For
multiplex RT-PCR, a degenerate antisense primer was designed and used in
conjunction with three virus-specific sense primers. The amplification efficiencies for the detection of the three viruses in the multiplex RT-PCR reaction were identical to those obtained in the single RT-PCR reactions for individual viruses (Saade et al., 2000). RT-PCR is the most sensitive test for the
detection of Banana bract mosaic virus (BBrMV) in banana plants followed
by the F(ab')2 indirect DAS-ELISA and IC-PCR (Rodoni et al., 1999).
The coat protein gene of Grapevine rupestris stem pitting-associated virus (GRSPaV) was amplified with primers based on a completely sequenced GRSPaV isolate. The protein expressed in Escherichia coli was
used to raise an antiserum in rabbit. This antiserum was used to detect the
virus in infected grapevine extracts by dot immunobinding (by spotting on
polyvinyl difluoride membranes) or by Western blot. ELISA was ineffective
in detecting the virus in grapevine (Minafra et al., 2000). Hailstones et al.
(2000) developed a specific seminested RTPCR assay that detects Citrus
tatter leaf virus in citrus trees. The sensitivity of the assay is at least 500
times greater than that of ELISA-based methods and allows detection directly from field trees.
DIAGNOSIS OF VIROID DISEASES

Bidirectional electrophoresis is used to test chrysanthemums for the
chrysanthemum stunt viroid. Molecular hybridization methods are also
used to detect this viroid (Dinesen and van Zaayen, 1996). Primer pairs and
nucleic acid preparations were used with RT-PCR to detect peach latent mosaic viroid (PLMVd) from stone fruits (Osaki et al., 1999). PCR methods
are useful in detecting hop stunt viroid in hops, apple scar skin viroid and
pear rusty skin viroid in apple and pear, citrus exocortis viroid in citrus, and
grape viroids in grapes (Henson and French, 1993).
DIAGNOSIS OF PHYTOPLASMA DISEASES
Phytoplasmas are detectable microscopically in phloem by means of
Dienes stain (Sinclair et al., 1996). The DAPI (4',6-diamidino-2-phenylindole-2HCL) test is more useful in the diagnosis of phytoplasmas (Seemller, 1976). DAPI binds to DNA and causes it to fluoresce under UV. When
longitudinal sections of twigs, petioles, or small roots of ash yellows-affected
Fraxinus and Syringa trees are treated with DAPI and examined with a fluorescence microscope, phytoplasmal DNA appears as blue-white fluorescent
specks or aggregations in sieve tubes, whereas normal sieve tubes remain
dark (Sinclair et al., 1996). The DAPI test is considered nonspecific because
DNA of any organism fluoresces under the test conditions.
Several DNA-based techniques are available to detect phytoplasmas.
PCR is highly useful in detecting several phytoplasmas. PCR primers that
are commonly used are based on sequences in the 16S ribosomal RNA gene
that are common to all phytoplasmas, but do not occur in plants (Lee,
Hammond, et al., 1993). A DNA segment of characteristic size is amplified
from any phytoplasma. The organism is then identified by using this initially amplified DNA segment as a template for further PCRs using primers
that amplify DNA from only particular phytoplasmas. These primers are
based on nucleotide sequences between the positions of the first primer pair
on the 16S rDNA (Lee et al., 1994).
In another method, the initial PCR product is subjected to restriction
fragment length polymorphism (RFLP) analysis, in which the amplified
segment is digested with certain restriction endonucleases and separated
into fragments by gel electrophoresis. Phytoplasmas in different groups
have different RFLP profiles (Guo et al., 2000). Ash Y phytoplasmas can be
distinguished from others by RFLP analysis of 16S rDNA with the restriction enzyme AluI (Lee, Hammond, et al., 1993).
Phytoplasmas can be diagnosed by means of DNA hybridizations using

probes that hybridize to group-specific sequences. Immunological tests
were developed to identify phytoplasmas. Monoclonal antibodies to peach
eastern X-disease agent have been developed and their use in disease detection has been demonstrated (Lin and Chen, 1985). Immunocapture PCR
tests have also been developed to diagnose phytoplasma diseases (Sinclair
et al., 1996).
PLANT CLINICS AND DIAGNOSTIC
SERVICE LABORATORIES
Several plant clinics have been set up in the United States and many other
countries, and they play an important role in crop disease diagnosis. Plant
clinics exist typically as part of a plant pathology department of a states
land grant university, as a part of a states department of agriculture, or as a
private or commercial service. Clinics operate throughout North America as
the primary source of diagnostic information and as the primary focal point
for the submission of plant-disease specimens (Barnes, 1994). Extensionuniversity-supported clinics and state department of agriculture clinics represent the backbone of disease diagnostic services. These clinics routinely
use ELISA-based diagnostic procedures and PCR technology. Many clinics
routinely use highly technical diagnostic procedures. The clinics provide
clients with an accurate diagnosis, and the diagnostic report is sent by mail,
fax, phone, modem, or computer network.
Several diagnostic service laboratories were established in the United
States since the 1970s. They charge a nominal fee for diagnosis. Plant disease clinics have an instructional component, whereas diagnostic service
laboratories simply diagnose plant diseases (Barnes, 1994).
DIGITALLY ASSISTED DIAGNOSIS
Diseases are recognized mostly by visual disease symptoms. Photographic images of plant disease symptoms and signs can be useful in diagnosis. Modern telecommunications systems permit individuals to share
high-resolution digital images among multiple locations within seconds
(Holmes et al., 2000). These digital-imaging and digital-image transfer
tools are used for crop disease diagnosis. Digitally assisted diagnosis permits long-distance consultation and accurate diagnosis of plant disease
problems. In the United States, the University of Georgia introduced in
1997 the Web-based delivery system for digitally assisted diagnosis, and
they named it Distance Diagnostics through Digital Imaging (DDDI).

County extension offices are provided with a computer, digital camera, dissecting and compound microscopes, and have access to the DDDI system.
In cases in which the disease shows unique symptoms, adequate background information is available, and a high quality digital image is obtained, accurate diagnosis can be made within minutes with the help of experienced technicians. It is now possible for the grower to send and receive
images from anywhere in the world, including fields, using cellular modems
(Holmes et al., 2000). Several Web sites, which describe digitally assisted
diagnostics programs, are now available: <http://www.dddi.org>, <http://
ddis.ifas.ufl.edu/>, <http://cf.uwex.edu/ces/ag/disdiag>, <http://www.ent.
iastate.edu/rdi>, <http://www.ca.uky.edu/agcollege/plantpathology/PPAExten/
digiimag.htm> (Holmes et al., 2000).
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CROP DISEASE EPIDEMIOLOGY
14
Assessment
Assessmentof
of Disease
Disease Progress
Progress
Epidemiology is the science that describes the progress of a disease as it

becomes epidemic. Depending on the rate of disease progress, diseases are
broadly classified as simple interest and compound interest diseases. Several models have been developed to describe the progression of these two
kinds of diseases. The area under disease progress curve (AUDPC) provides
a valid statistical description of disease progress data.
WHAT IS EPIDEMIOLOGY?
A widespread temporary increase in the incidence of an infectious disease is called an epidemic. A plant disease is described as epidemic when
the amount of disease present increases rapidly from a low level to a high
one. This is in contrast to an endemic disease, which is a disease established
in moderate or severe form in a defined area. For an endemic disease, the
disease level remains almost constant.
The study of epidemic diseases is known as epidemiology. Epidemiology
describes the rapid multiplication of the invading pathogen and the subsequent increase in disease levels within a large population of plants that have
no immunity, but varying levels of resistance, to the particular pathogen.
Epidemiology comprehensively analyzes the interaction between the constituents of the disease triangle: the host, the pathogen, and the environment.
Epidemiology is concerned with diseases in populations, that is, with
host populations interacting with pathogen (or vector) populations. Epidemiology describes the host population and pathogen population and the effect of the environment on these populations. The primary goal of epidemiology is to understand the factors that influence disease development,
develop efficient disease management strategies, and reduce losses due to
diseases (Van der Plank, 1963; Leonard and Fry, 1986; Manners, 1993;
Campbell, 1998).
DISEASE PROGRESS CURVE

The amount of disease present on the plant varies with time, and a rapid
increase in disease incidence and severity with time indicates development
of an epidemic. The progress of a disease may be assessed as disease incidence (proportion of diseased plants in a plant population) or as disease severity (proportion of diseased tissue in a plant). When the results are plotted
against time, the curve obtained is commonly S-shaped. This curve is
known as the progress curve of the disease.
Van der Plank (1963) classified diseases into two kinds, based on the disease progress curve: simple interest diseases and compound interest diseases. In simple interest diseases, only one cycle of infection and inoculum
production per growing season occur (i.e., they are monocyclic). These diseases are called simple interest diseases because the rate of disease increase
follows simple interest pattern. Simple interest diseases are initiated from a
given inoculum quantity at the beginning of the disease cycle and multiply
once per season, usually to leave a legacy of a much higher quantity of
inoculum in the soil or on planting material in readiness for the next seasons crop. In these diseases, the amount of inoculum is constant throughout
the season, even though the number of plants infected or the plant tissue area
affected may rise. Examples for these diseases include wilts, some root rots,
and internally seedborne smut diseases, such as loose smut of wheat.
In compound interest diseases, cycles of infection and inoculum production occur repeatedly, and thus many disease cycles occur during a growing
season (i.e., they are polycyclic). Compound interest diseases multiply rapidly in a season with several generations of spore production. These diseases are called compound interest diseases because the disease increase
follows the pattern of a banks compound interest calculation. Examples for
these diseases include rusts, powdery mildews, and other foliar diseases.
Van der Plank (1963) developed two biological models, a monomolecular model and a logistic model, for describing the progression of simple interest and compound interest diseases. The monomolecular model describes
simple interest diseases, whereas the logistic model describes compound interest diseases. The monomolecular model can be written as:
dy
= k (1 y )
dt
where dy/dt is the absolute rate of disease increase, k is the rate parameter,
and y is the proportion of increase at time (t). The maximum value that y can
reach is assumed to be 1. The model assumes that the absolute rate of dis-
ease increase is proportional to the amount of healthy tissue remaining at

any given time.
The logistic model can be written as
dy
= ky(1 y ).
dt
In this case, the absolute rate of disease increase is assumed to be proportional to the amount of disease and of healthy tissue present.
Another important model developed to describe the disease progress
curve is the Gompertz model. It is written as
dy
= rg y (In( k ) ) In( y )
dt
where rg is the rate of increase specific to the Gompertz model, k is the maximum level of disease, y is the disease at time of observation, and t is the time
interval being considered, and the symbol In indicates natural logarithm.
These three models are often used in a linearized form. The disease progress curves are transformed to a linear form with the assumption that the
maximum amount of disease, (ymax = k), is 100 percent or proportionally
1.0. Linear regression analysis is then used to provide estimates of the initial
amount of disease (yo) and the apparent rate of increase (r). The appropriateness of a model for describing disease progress data is then judged by goodness-of-fit criteria, including the magnitude of the coefficient of determination (R2) and mean square error (MSE) (Campbell, 1998).
Linear models are more useful than nonlinearized models (e.g., the
monomolecular, logistic, and Gompertz models) because the software is
able to perform linear regression analysis quite easily. However, software
for nonlinear regression is now available. The fitting of linear and nonlinear
regression models is based on the same idea, i.e., choosing parameter estimates that minimize the sums of squared errors. Since fewer assumptions
are made with nonlinear regression analysis, this analysis is preferred for
disease progress data (Campbell, 1998).
Other models employed less frequently to assess the disease progress
data include the Weibull, Richards, and Gaussian models. These models are
used to calculate the rate of disease increase, and in some cases meaningful
and more or less constant r values can be obtained. However, in many cases
frequent changes in r values occur due to large fluctuations in environmental factors. Data on environmental factors have not been incorporated in
these models for analysis.
Many limitations exist in using the Weibull, Richards, and Gaussian
models. For example, they may not be sufficient to describe all disease pro-
gress curves. If the weather becomes unfavorable for disease development

during the course of an epidemic, a plateau may occur within the disease
progress curve. When new flushes of host growth due to agronomic factors
occur, the disease progress may decline. These models can be useful in
regions where weather conditions are reasonably constant throughout the
period under examination, and where no appreciable number of leaves or
plants are added or removed.
The disease progress curves are useful to assess the development of an
epidemic. In the long run, a high rate of interest is more important than a
large balance in the bank today (Van der Plank, 1963). Similarly, a high rate
of increase (r) will contribute to an epidemic. A modest decrease in r may
prevent an epidemic.
AREA UNDER DISEASE PROGRESS CURVE (AUDPC)
The area under disease progress curve (AUDPC) is an alternative method
that provides a valid statistical description of disease progress data. AUDPC
is the amount of disease integrated between two times of interest, and is calculated without regard to curve shape (Campbell, 1998). It is a valid
descriptor of an epidemic under the hypothesis that injury to a host plant is
proportional to the amount and duration of the disease. AUDPC is calculated by using the following formula:
n
AUDPC = [( x i + 1 + x i ) / 2]( t i + 1 t i )
i =1
where xi = proportion tissue affected (disease intensity) at the ith observation, t = time (days) after inoculation at the ith observation, and n = total
number of observations. is the sum of areas of all of the individual trapezoids or areas from i to n 1. i and i + 1 represent observations from 1 to n
(Shaner and Finney, 1977).
If the epidemic duration differs, AUDPC values are normalized by dividing the AUDPC by the total area of the graph (= the number of days from inoculation to the end of the observation period 1.0). The normalized
AUDPC is referred to as relative AUDPC. Units for AUDPC are days
proportion or days percent, depending upon the measure of disease intensity. Relative AUDPC has no units. Greater frequency of disease assessments over time result in a more accurate estimate of AUDPC than when
only a few assessments are made.
APPARENT INFECTION RATE

The apparent infection rate is useful in assessing the disease in the field.
It is calculated as follows (Van der Plank, 1963):
r = 1 / t 2 t l log e [ x 2 (1 x l ) / x1 (1 x 2 )]
where x = the proportion of disease at any one time, x1 = the amount of disease present at time t1, x2 = the amount of disease present at time t2, t = time
during which the infection has occurred, e = base of natural logs, and r = the
average infection rate/apparent infection rate.
REFERENCES
Campbell, C. L. (1998). Disease progress in time: Modelling and data analysis. In
D. G. Jones (Ed.) The Epidemiology of Plant Diseases. Kluwer Academic Publishers, Dordrecht, The Netherlands, pp. 181-204.
Leonard, K. J. and Fry, W. E. (1986). Plant Disease Epidemiology, Population Dynamics and Management, Vol. 1. Macmillan Publishing Co., New York, p. 372.
Manners, J. G. (1993). Principles of Plant Pathology. Cambridge University Press,
Cambridge, p. 343.
Shaner, E. and Finney, R. E. (1977). The effect of nitrogen fertilization on the expression of slow-mildewing resistance in Knox wheat. Phytopathology, 67:
1051-1056.
Van der Plank, J. E. (1963). Plant Diseases: Epidemics and Control. Academic
Press, New York, p. 349.
15
Factors
Factors
Involved
Involved in
in Disease
Disease
Increase
Increase
Several environmental factors are involved in the temporary widespread
increase in fungal and bacterial diseases (i.e., epidemic). The life of a fungal
pathogen may be divided into sporulation, dispersal (removal, transport,
and deposition), germination, infection, and incubation phases. Environmental factors involved in all phases of the fungal life cycle are discussed.
Environmental factors affecting bacterial spread and infection are also described.
ENVIRONMENTAL FACTORS AFFECTING SPORULATION
Sporulation of fungal pathogens in the host appears to be influenced by
weather factors. The production of ascospores of Mycosphaerella citri on
citrus leaves is greatest from March to July, and few ascospores are produced
thereafter in Florida (Timmer, Roberts, et al., 2000). Moisture, temperature,
humidity, and light influence sporulation. Asexual spores (pycnidiospores)
of Septoria tritici are important in the epidemiological development cycle
of the pathogen in wheat. The production of pycnidiospores is strongly dependent on moisture and temperature (Verreet et al., 2000). Sigatoka (yellow
Sigatoka) and black leaf streak (black Sigatoka) pathogens (M. musicola
and M. fijiensis, respectively) produce conidia abundantly in wet weather or
in dew on the surface of leaf lesions (Burt et al., 1999). Sporulation of the
apple sooty blotch pathogens Peltaster fructicola and Leptodontium elatius
was greatest at relative humidities of 97 to 99 percent (Johnson and Sutton,
2000). The optimum temperature for sporangial production of Phytophthora palmivora on a citrus fruit surface was 24C, with sporangial production decreasing rapidly at higher or lower temperatures (Timmer, Zitko, et
al., 2000). A few sporangia were produced with 18 h of fruit wetness, and
numbers increased as the duration of wetness increased to 72 h (Timmer,
Zitko, et al., 2000). Sporulation on soybean root surfaces infected with
Fusarium solani f. sp. glycines is more frequent during or immediately following high moisture in soil (Roy, 1997). Temperature and moisture influence the apothecium production of Sclerotinia sclerotiorum, the stem rot
pathogen of soybean. High light intensity favors the development of apothecia (Sun et al., 2000). Moderate temperatures (14 to 28C) combined with
high relative humidity ( 90 percent) are conducive to conidial production of
Claviceps sorghi (Bandyopadhyay et al., 1998). Sporulation of Pseudocercosporella herpotrichoides, the wheat eyespot pathogen, may occur
within the temperature range 1 to 20C, with an optimum temperature of
5C (Fitt et al., 1988). Sporulations of the peanut leaf spot pathogens Cercospora arachidicola and Phaeoisariopsis personata occur on lesions, and
sporulation is favored by high relative humidity (about 100 percent RH) and
temperatures ranging from 24 to 28C. Sporulation increases with increased
wet period duration (Jacobi et al., 1995). Temperature and wetness duration
have the greatest effect on inoculum production of Botrytis cinerea, the
strawberry gray mold pathogen. The optimum temperature for sporulation
in dead leaf tissue is 18C, and sporulation increases with longer wetness duration (Sosa-Alvarez et al., 1995).
ENVIRONMENTAL FACTORS AFFECTING SPORE RELEASE
The spore dispersal phase of the pathogen life cycle has three stages: removal, transport, and deposition. Rain, wind, humidity, and temperature
may play important roles in spores release from the host surface. Conidia of
the wheat powdery mildew pathogen Erysiphe graminis may be removed
from infected leaves by wind gusts or by acceleration forces as leaves flap in
the wind. They may also be removed by raindrops, through puff or tap
mechanisms. As a raindrop spreads out on a dry leaf, it generates a puff of
air that may remove dry conidia. The impact (tap) of the raindrop on the leaf
may also release dry spores as kinetic energy is transferred to the leaf. Dry,
airborne conidia of E. graminis are a common component of air spora during dry summer weather in the United Kingdom (Fitt and McCartney,
1986).
Pycnidiospores of Septoria nodorum are generally produced in mucilage, which prevents their removal from infected wheat plants by wind.
However, during rainy weather, the first raindrops dissolve the mucilage to
leave a spore suspension that is prone to splash dispersal by subsequent
raindrops (Fitt and McCartney, 1986). Lighter or shorter rainfalls enhance
the removal of urediniospores of the wheat leaf (brown) rust pathogen
Puccinia triticina from wheat leaf surfaces (Sache et al., 2000). Ascospores
of the sigatoka pathogen Mycosphaerella fijiensis are produced on older banana leaves in perithecia sunken into leaf tissues. Under wet conditions,
spores are released and are dispersed by air. Rainfall, combined with a high
temperature, may lead to peaks of ascospore release (Burt et al., 1999).
The release of large numbers of conidia of the citrus brownspot pathogen
Alternaria alternata from citrus leaves was triggered by sudden drops in relative humidity or by simulated rainfall events. Vibration induced the release
of low numbers of conidia (Timmer et al., 1998). The release of ascospores
of Anisogramma anomala was monitored over a six-year period in European hazelnut orchards in the United States. The ascospores that were released were correlated with the amount of precipitation (Pinkerton et al.,
1998). A suction-impaction mini-spore trap was developed to study the
effect of light initiation and decreasing relative humidity (RH) on spore release of the lettuce pathogen Bremia lactucae in a controlled environment.
Three light periods (from 0400 to 1600, 0600 to 1800, and 0800 to 2000 h,
circadian time) at a constant RH of 99 to 100 percent were used for studying
the effect of light initiation on spore release (Su et al., 2000). Only a few
spores were released during the dark periods. Spore release increased
sharply after the initiation of light periods and reached a maximum 1 to 2 h
after light initiation. When relative humidity (RH) decreased from 100 to 94
percent two hours before light initiation, spore release increased within 1 h.
These results suggest that both light initiation and reduction in RH can trigger spore release of B. lactucae (Su et al., 2000).
The release of ascospores of the grapevine powdery mildew pathogen
Uncinula necator was monitored under natural conditions in France during
a five-year period (Jailloux et al., 1999). Ascospore release always began after bud burst and generally ended before blossoming. Ascospore release
was always associated with a rainfall higher than 2 mm, a wetting duration
greater than 2.5 h, an average temperature generally above 11C, and a daily
mean temperature sum from November 1 to the first ascospore release
above 1100C (Jailloux et al., 1999). The release of ascospores of the sour
cherry black knot pathogen Apiosporina morbosa was dependent on rainfall
and temperature, but not on the duration of wetness (McFadden-Smith et al.,
2000). Byrne et al. (2000) showed that fluctuations in relative humidity (either positive or negative) increase the release of conidia of Oidium sp. in
poinsettia under greenhouse conditions in the United States. Watering resulted in an immediate increase (25 percent) followed by a rapid decrease in
RH (32 percent) beginning 1 to 2 h later. Conidial release increased by about
89 percent within 3 h following greenhouse watering (Byrne et al., 2000).
Cool, wet weather in the fall and spring provides conditions conducive to
ascospore release of Sclerotinia sclerotiorum, the cabbage stem rot pathogen (Hudyncia et al., 2000).
ENVIRONMENTAL FACTORS
AFFECTING SPORE DISPERSAL
Wind and rain are the important factors involved in spore dispersal.
Urediniospores of Puccinia triticina are responsible for epidemics of leaf
rust. The spores are wind-borne. Local dispersal of urediniospores within a
wheat canopy from an infection focus occurs most efficiently (dispersal to
greater distances before becoming deposited) when the wind direction is
perpendicular to the wheat rows, rather than when it is parallel to them. The
resistance of wheat plants creates a turbulence that tends to lift spores into
the airspace just above the canopy and keeps them suspended for a longer
period of time. This may allow the spores to travel further before settling
back into the canopy and being deposited on the tissues of wheat plants or
the soil beneath them (Kramer and Eversmeyer, 2000). Light rain is able to
wash airborne Puccinia triticina spores from the atmosphere and deposit
them on wheat leaves. However, violent or extended rain events inhibit further spore production and removal for more than six hours. Rain can clear
out the spore load in the atmosphere within 50 minutes. Susceptible trap
plants exposed during violent rain events are much less infected by rain than
those sheltered from rain, indicating that rain is able to wash off spores already deposited on leaves by incidental raindrops (Sache et al., 2000). Thus,
rainfall patterns may decide the spread of the spores.
The urediniospores of the wheat stem rust pathogen Puccinia graminis f.
sp. tritici are carried long distances by the wind. In the United States, it has
been reported that the spores can travel more than 1,000 miles in about two
days. Spores from Texas spread throughout the Midwest and into Canada
annually (Roelfs, 1985). Rust spores are carried by wind from Australia to
New Zealand. In India, urediniospores from South India are carried through
cyclonic wind to North India, crossing thousands of miles; the path is called
Puccinia path. Seasonal rain in India washes the spores from the atmosphere
and deposits them on wheat leaves.
Venturia inaequalis, the apple scab pathogen, survives the winter in diseased apple leaves on the ground. Pseudothecia and ascospores develop in
these fallen leaves. Ascospores produced on diseased leaves in the leaf litter
are released into the air at ground level from ascus. The great majority of ascospores are propelled less than 1 cm into the air. Once airborne, they are simultaneously transported downwind by the horizontal motion of the wind
and diffused vertically by turbulent fluctuations. A portion is also deposited
on the vegetation and ground. Spore concentration decreases rapidly with
height above the ground and with increasing downwind distance from a
source. The rapid decrease of ascospores in the air with increasing distance
and height is due to wind shear, turbulent diffusion, and losses due to washout by rain. The ascospores are deposited onto plant surfaces either by raindrops containing ascospores or by impaction and sedimentation of ascospores that are contained in the air (Aylor, 1998). Rain plays a major role in the
long-distance (several km) transport of ascospores. Rain tends to reduce the
effect of spore dilution in the vertical direction because raindrops sweep
through the entire column of air containing spores and bring the spores to the
ground (Aylor, 1998). The effect of rain on ascospore dispersal was further
studied in Italy in detail (Rossi et al., 2000). Rain events were the only occurrences that allowed ascospores to become airborne. A rain event is a
period with measurable rainfall of 0.2 mm/h lasting one to several hours,
uninterrupted or interrupted by a maximum of two dry hours (Rossi et al.,
2001). In the absence of rain, dew is insufficient to allow ascospores to disperse
into the air. In some cases, rain events did not cause ascospore dispersal, including when rain fell at night and was followed by heavy dew deposition
that persisted some hours after sunrise (Rossi et al., 2001). The intensity of
rain may determine the amount of spores dispersed. A rain density of 30 mm/h
results in significantly greater rain-splash dispersal of Colletotrichum acutatum spores infecting strawberry (Ntahimpera et al., 1999).
The pycnidiospores of the glume blotch pathogen Stagonospora nodorum (Septoria nodorum) are dispersed by rain splash from lower infected
leaves to successively higher (younger) leaves (De Wolf and Francl, 2000).
Vertical and horizontal spread of conidia of Septoria tritici, a wheat pathogen, takes place through the kinetic energy of striking raindrops. Dispersal
and infection takes place when total precipitation exceeds 10 mm within
three days, or total precipitation exceeds 5 mm over two days, and there exists a continuous three-day-long leaf wetness period (Verreet et al., 2000).
The conidia of the banana sigatoka pathogen Mycosphaerella fijiensis are
dispersed by water droplets to other leaves (Burt et al., 1999).
MEASUREMENT OF SPORE DISPERSAL
The accuracy of a spore dispersal model depends, to a large extent, on the
quality of the experimental data on which it is based or with which it is
tested. Spore samplers (spore traps) are used for estimating the number of
airborne or splash-borne spores. Different types of spore samplers are available. Spore samplers may collect spores on dry, sticky surfaces or in liquids.
They may be passive, collecting the spores that reach them, or volumetric,
sampling a known volume of air. The following are important spore traps.
Dry samplers:
Passive spore trapsHorizontal slide, vertical cylinder, volumetric
cylinder
Volumetric spore trapsBurkard, Cascade impactor, Rotorod, Hirst
Liquid samplers:
Passive spore trapsFunnel
Volumetric spore trapsCyclone, Impinger
In the horizontal slides and volumetric cylinders, spores are collected on
a surface coated with an adhesive such as Vaseline, on which spores do not
germinate. In Hirst and Burkard spore traps, air is sucked into the traps at a
controlled rate and impinged onto a sticky surface moved by a clockwise
mechanism past the orifice. Thus, the number of spores per unit volume of
air at any given time can be calculated.
The Cascade impactor is similar to the Burkard trap in its function. The
Rotorod sampler consists of a U-shaped rod attached at its midpoint to the
shaft of a small, battery-operated electric motor. The surface of the rod is
covered with a Vaselined strip of transparent cellophane, which can be
stripped off and mounted. From the area of the strip and the speed of rotation, the volume of air sampled can be calculated. In Cyclone and Impinger
spore traps, spores are impacted by air suction into the samplers.
Living plants are also used as spore traps. The number of pustules developed on plants exposed for a given time (usually 24 h) and then returned to a
clean air chamber for incubation are counted to assess the spore load in the
air. A mobile nursery containing seedlings of the differential hosts for the
pathogen is exposed in the field for a suitable time, and the plants are removed to optimum concentrations for incubation in a spore-proof glasshouse. The physiologic races to which any pustules belong are then recorded.
The choice of spore sampler depends on biological factors such as the
mode of spore spread, spore size, and spore concentration in the air. When
sampling above crops, it is necessary to use an inertial sampler to collect dry
airborne spores. Vertical cylinders, Burkard traps, and Hirst traps collect
many more spores per square centimeter than horizontal slides. Because
impaction efficiencies of small spores are small, a volumetric sampler
(Burkard, Hirst, Cascade impactor, Rotorod) will sample spores better than
a passive vertical cylinder, particularly at low concentrations and low wind
speeds.
Venturia inaequalis ascospores (measuring 14 7 m) can be collected

more easily using Burkard and Rotorod samplers than using sticky cylinders. However, simple cylinders are appropriate samplers for spores such as
those of Erysiphe graminis, which are large (30 15 m), present in high
concentrations, and dispersed at high wind speeds. Volumetric suction samplers may underestimate concentrations of the largest pathogen spores, such
as those of Helminthosporium and Alternaria, and the cylinders may be
better samplers for them.
In choosing samplers for spores carried in splash droplets, the size of the
droplets is more important than the size of the spores themselves. The range
of particle sizes to be sampled is greater than those of dry airborne spores.
Spore-carrying droplets may be 10 to 1,000 m in diameter, and different
samplers may be required for the efficient sampling of different sizes of
droplets. Spores of many splash-borne pathogens are mostly carried in large
ballistic droplets. Samplers appropriate for dry airborne spores are generally unsuitable for collecting large spore-carrying droplets because the
spores can be washed off vertical sampling surfaces. Horizontal slides (under rain shields to prevent wash off), funnels (draining into beakers), and
impingers collect more such spores. Slides probably lose some spores
through runoff. Funnels collect most spores, but the liquid samples require
concentration before spores can be counted. The impinger collects more
spores per square centimeter than the funnel, but requires power to operate
its suction pump. Small, airborne spore-carrying droplets, which are generally present in low concentrations, would not be collected efficiently by
samplers with horizontal sampling surfaces near ground level. Volumetric
suction samplers, such as impingers or high volume cyclone separators, are
more appropriate.
The positioning of spore samplers (traps) in the field depends on the position of the source of spores, the mode of spore dispersal (especially
whether spores are airborne or in ballistic splash droplets), and the type of
samplers used. The number of spores of Erysiphe graminis collected by suction samplers, which measure the airborne spore concentration, decreased
rapidly with height above a barley crop (Bainbridge and Stedman, 1979).
Similarly, the numbers of spores collected on horizontal slides (mostly by
impaction) increased. This increase was due to the increase in wind speed
with height, and these differences between samplers illustrate that it is important to understand the sampling characteristics of a device before interpreting the results it gives (Bainbridge and Stedman, 1979). All three sampler types, i.e., suction samplers, vertical cylinders, and horizontal slides,
collected fewer spores when positioned further upwind or downwind of the
barley crop. Such decreases in the number of spores collected with a distance, or spore dispersal gradients, are extremely important in disease
spread and in epidemiological models. The upwind gradient was steeper

than the downwind gradient, which is usually the case for airborne spores.
Splash-dispersed spores usually show a steep gradient, both with height
above and with distance away from a source (Bainbridge and Stedman,
1979).
Therefore, for measurement of background airborne spore concentrations, samplers should be placed above and away from active sources of
spores. For measurement of spore dispersal gradients, they should be close
to the source, particularly when splash-dispersed spores are being studied.
With ground-level sources, samplers must be near the ground. When spore
dispersal within crops is of interest, they should be positioned so that there
is minimum disturbance to the crop, although there must be some gap
around the sampler so that infected leaves do not rub against it.
The decreases in the concentrations of spores with distance from and
height above a strip of infected plants suggest the existence of spore dispersal gradients. The decreases in spore concentration with distance away
from a source result in spore deposition gradients as portions of the spore
clouds are deposited on the ground or crops. These gradients are important
in determining the rate of spatial spread of epidemics.
DISEASE GRADIENTS
Although disease progress curves represent disease development in time,
disease gradients represent disease development in space. The nature of a disease gradient in any one disease depends on the dispersal method adopted by
the pathogen and on the nature and stage of development of the host. A disease gradient will occur when the primary source of infection is a focus.
This may consist of what is virtually a point source, such as a single lesion,
of an area, a patch of disease within a crop, or of virtually a line, such as a
hedgerow. When the source is uniformly distributed over a crop, as is the
case with a cloud of spores blown by the wind from a large, distant source,
no gradient will occur.
Various equations have been used to describe disease gradients (Mundt,
1989). The following two formulae are widely used.
The inverse power law: y = as b
The negative exponential law: y = ae bs
For these formulae, y is the amount of disease at a distance (s) from the
source, a is a function of the total disease amount, b is the slope of the line,
which is obtained when the data are transformed (using a log log or log
linear transformation) to obtain an approximation to a straight line, and e is

the basis of logarithms.
Gradients for some dry airborne spores fit the power law model better
than the exponential model, while data for others fit an exponential model
better. Such gradients are always steep. When multiple foci occur, the analysis of disease spread is complex (Mundt, 1989).
ENVIRONMENTAL FACTORS AFFECTING SPREAD
OF BACTERIAL PATHOGENS
Xanthomonas axonopodis pv. citri, the citrus canker bacterial pathogen,
appears in rainwater running over the surfaces of lesions and splashing onto
new shoots. The pathogen enters the host tissues through stomata or wounds.
Storms such as typhoons or hurricanes encourage outbreaks of citrus canker. Rainstorms favor distant dispersal of the bacteria. The pathogen can be
dispersed in small raindrops also. Disease spread is independent of wind direction because rain-splash dispersal and rapid development of secondary
foci of disease prevail. In contrast, windblown rain is responsible for the
highly directional spread from a disease focus in the orchard. Subsequent
spread from secondary loci is rapid and in many directions (Graham and
Gottwald, 1991). Young citrus canker lesions rapidly exude up to 105-106
colony forming units per lesion within hours after wetting. Wind-driven rain
and water-soaking of tissue appear to be essential for dissemination and ingress of bacteria and for epidemic development of citrus canker.
In the case of rice bacterial blight caused by Xanthomonas oryzae pv.
oryzae, bacterial cells ooze from fresh lesions whenever dew or rain occurs.
The drops dry to become minute beads that easily fall into water in the field.
The bacteria are carried by irrigation water from field to field. Splash dispersal of the pathogen has been demonstrated (Mundt et al., 1999).
ENVIRONMENTAL FACTORS AFFECTING INFECTION
AND DISEASE DEVELOPMENT
The severity of citrus Alternaria leaf and fruit spot disease is positively
related to the amount of rainfall, duration of leaf wetness, and average daily
temperatures (Timmer et al., 1998). Moisture appears to be a critical element in favoring Tilletia indica infection of wheat kernels. Teliospore germination requires at least 82 percent relative humidity and, preferably, free
water. Several rainy days during the flowering stage favor disease incidence.
The longer the period of high relative humidity and rainy weather, the greater
the number of seeds infected. Moisture at the time of flowering is the most
critical factor in establishment of the disease (Bonde et al., 1997).
Wetness duration, temperature, and light conditions determine the epidemic of wheat stripe rust (Puccinia striiformis) disease. Rain events also
determine disease incidence. Light and short rainfall enhances disease incidence, whereas heavy violent and extended rain events decrease disease
incidence (Vallavieille-Pope et al., 2000). Under high temperature and prolonged leaf wetness periods, infection by Mycosphaerella fragariae becomes severe in strawberry. The number of lesions on young leaves increases gradually from 5 to 25C and decreases sharply from 25 to 30C.
The optimal temperature for infection is 25C. But for most temperatures, a
minimum of 12 h of leaf wetness is necessary for infection (Carisse et al.,
2000).
Relative humidity and rainfall appear to contribute to the development of
wheat leaf and glume blotch disease epidemics (De Wolf and Francl, 2000).
Leaf wetness periods of more than 48 h and relative humidity greater than
98 percent result in epidemics of the disease caused by Septoria tritici in
wheat (Verreet et al., 2000). The optimal temperature of pycnidiospore germination is in the range of 22 to 24C. Infection is high at a temperature between 16 and 21C. Around 7C, infection is inhibited (Verreet et al., 2000).
The optimal temperature for development of potato common scab disease
(Streptomyces scabies) is about 20C. Low soil moisture increases disease
incidence. Soil characteristics can greatly affect the severity of potato scab.
Scab is most severe in soils with a pH of 5.2 to 7.0 (Loria et al., 1997).
Ascospores of Monosporascus cannonballus germinate in cucurbit rhizosphere. Optimum germination occurs at temperatures ranging from 25 to
35C. The ascospore population within 500 m of a root is capable of germination and subsequent penetration of cantaloupe roots. The fungus multiplies in the root tissues and brownish lesions appear (Stanghellini et al.,
2000). Rainfall greater than 10 mm and a maximum temperature greater
than 5C during this favorable period can result in peach leaf curl (Taphrina
deformans) disease incidence (Giosue et al., 2000). Daytime vapor pressure
deficit and nighttime temperature have the greatest effect in strawberry
flower infection by Botrytis cinerea. Infection is favored by low day vapor
pressure deficit and high night temperature (Xu et al., 2000).
The optimum temperature for germination of conidia of Cercospora
arachidicola, the peanut early leaf spot pathogen, is 19 to 25C, and the
conidia require free water for germination. Periods of leaf wetness exceeding 10 h and minimum temperatures during the wetness period above 21C
are the most crucial conditions for early leaf spot disease development
(Jacobi et al., 1995). High relative humidity favors development of pear leaf
scab caused by Venturia pirina. The length of the dry period reduces disease
severity. No scab lesions occur if leaves are dry for more than 12 h (Villata
et al., 2000b). Disease severity increases with increasing leaf wetness duration. The optimum temperature for infection is 20C (Villata et al., 2000a).
When the winter is cold and rainy, the onset of V. pirina epidemics occurs
earlier in the season, and disease intensity reaches the highest levels. In
warm and less rainy winters, the onset of epidemics is delayed and the epidemic is less extensive (Sobreiro and Mexia, 2000).
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16
Forecasting
Forecasting Models
Models
Several disease-forecasting models were created to help develop decision support systems for timing chemical applications to control fungal and
bacterial diseases. The important forecasting models developed are described here.
A model is any representation of an object, system, or idea in some form
other than that of the entity itself. Models are by necessity limited sections
of reality, having some but not all of the properties of real-life situations.
Models are seldom complete, and modeling is an iterative (repetitive) process in which the model provides a closer and closer approximation of reality with each successive iteration. Thus, a model is never an end point in itself (Teng, 1985).
TYPES OF MODELS
Analytic Models
Different types of epidemic models exist, including analytic, simulation,
empirical, and hybrid models. Analytic models are used to analyze epidemics on a theoretical basis without taking into consideration the effects of external variables. They are mathematical models that attempt to describe epidemics as a whole by single mathematical equations. They are also called
statistical models or biological models. Statistical models are mathematical
formulas with parameter values that have been chosen to adequately describe disease progress for specific data sets, but lack a precise biological interpretation. Weibull, Richards, Gompertz, and Gaussian models are also
mathematical models. Biological models are also mathematical models but
are based on prior assumptions about the mechanism of disease increase.
Monomolecular and logistic models are biological models. These models
are mostly theoretical or simple regression models. They are also called
synthetic models by Van der Plank (1984).
Simulation Models
Simulation is the process of designing a model of a real system and conducting experiments with this model for purposes of either understanding
the behavior of the system or evaluating various strategies for the operation
of the system (Teng, 1985). Simulation models try to mimic observed epidemics or certain aspects, such as spore dispersal, that are influenced by
measured meteorological conditions (Hau, 1990). Simulation models are
generated by creating conditions for the development of epidemics and carrying out experiments on different aspects of disease increase. In the simulation approach, differential equations are composed by considering the life
cycle of fungal pathogens, by compartmentalizing the various components
of the life cycle (e.g., sporophores, spores, dispersed spores, lesions), and by
specifying the rates of change of each variable (Leonard and Fry, 1986). For
example, a simulation model to analyze the epidemiology of potato late
blight was developed (Bruhn and Fry, 1981). Both the number of lesions and
the mean surface area of lesions in each age class were simulated in response to the environment, host factors including cultivar resistance, and
fungicide application. The simulation model included the deposition and
weathering rate of the fungicide chlorothalonil, the fungicide application
method and dosage, interactions with plant growth and architecture, and environmental effects such as rainfall. Simulation models are concerned only
with particular, or a set of particular, behaviors and hence may not be suited
for the development of theories concerning the real system.
Empirical and Hybrid Models
These models are based on actual experiments and observations and not
on theory or simulation. Empirical models are complex models that attempt
to mimic many environmental factors and other influences.
Hybrid models that combine analytic and simulation approaches are also
being developed.
SPECIFIC MODELS
Potato Late Blight Forecasting Models
Several models were developed to forecast late blight of potato (Phytophthora infestans). The earliest forecasting model was developed by van
Everdingen (1926). The model is based on four criteria known as Dutch
rules. The Dutch rules prescribe the following conditions to indicate the
time to apply control measures against the disease: the occurrence of dew
for at least four hours at night, a minimum temperature of 10C, a mean
cloudiness on the next day of 0.8 or more, and at least 0.1 mm of rain in the
following 24 h. The Dutch rules were initially used in the United Kingdom.
Beaumont (1947) modified these rules to state that when there are two days
with a minimum temperature not less than 10C and relative humidity not
below 75 percent, the growers in the vicinity should begin routine spray programs. These two days were recognized as the Beaumont period, and it became the standard blight warning system in the United Kingdom until the
mid 1970s.
Irish rules, formulated by Bourke (1953), prescribe the following criteria to be satisfied for an outbreak of late blight: a humid period of at least
12 h with the temperature at least 10C and relative humidity (RH) 90 percent or above (conditions favorable for sporangia formation), and free moisture on the leaves for a subsequent period of at least 4 h (conditions favorable for germination and reinfection). If there is no rainfall, the alternative
requirement is for a further 4 h beyond the initial 12 h with an RH of at least
90 percent.
Smith (1956) came out with another model describing the critical two
days to forecast the late blight epidemic. Temperature and humidity requirements of disease development have been deduced after several years of
studies, and have been incorporated into Smith periods. A Smith period is
defined as two consecutive days (ending at 0900 hours) when the temperature has not been less than 10C and the relative humidity has been above
90 percent for at least 11 hours of each day. In the United Kingdom, Smith
periods are used to forecast disease incidence. Notifications of Smith periods are issued by the Agriculture Development Advisory Service (ADAS)
of the British government and communicated through television, bulletins,
and farming press.
In the United States, a computerized forecast called BLITECAST was
developed (Krause et al., 1975). It is based on temperature and relative humidity thresholds. Data required for BLITECAST include daily maximum
and minimum temperatures, the number of hours when relative humidity is
equal to or above 90 percent, the maximum and minimum temperature during the period when RH is 90 percent and above, and the daily rainfall figure
to the nearest 1 mm. The program is available on microcomputer, which is
coupled to a weather data logger. The system is available in the potato field
itself. BLITECAST advises not only the date to begin spraying, but also the
timing of subsequent applications.
In Denmark, Hansen et al. (1995) developed a forecasting model called
NEGFRY. The model takes into account cultivar susceptibility, emergence
date, and irrigation to advise on the date of first fungicide application and
timing of subsequent sprays. The BLITECAST system was modified by incorporating host resistance and fungicide weathering, and this system was
called SIM-CAST (Fry et al., 1983). SIM-CAST uses a decision rule similar
to that of BLITECAST, i.e., accumulating disease severity values, but does
not forecast the occurrence of the first spray (Grunwald et al., 2000). The
SIM-CAST system was equally effective when compared to BLITECAST
(Hijmans et al., 2000). Similar systems have been reported in several European countries. The BLITECAST system can also be used in tomato to forecast late blight in this crop.
Tomato Early Blight Forecasting Models
A forecaster of Alternaria solani on tomato (FAST) was developed to
identify periods when environmental conditions are favorable for early
blight development and provide an efficient fungicide application schedule
(Madden et al., 1978). Daily weather data (maximum and minimum air temperature, hours of leaf wetness, maximum and minimum temperature during wet periods, hours of relative humidity more than 90 percent, and daily
rainfall) are used for forecasting. FAST consists of two submodels: the dew
model and the rain model. Each submodel calculates a daily rating of the severity of risk of an early blight outbreak. The dew model is based on the duration of wet periods and average air temperature during the wet periods.
The hours of leaf wetness and mean air temperature during the wet period
are combined to derive disease severity (S) values. The S values are rated
daily from zero (conditions unfavorable for A. solani spore formation) to
four (conditions highly favorable for spore formation). The rain model uses
average air temperature for the last five days, total hours during the past five
days with RH 90 percent, and total rainfall for the past seven days to calculate disease severity rating values from zero (conditions unfavorable for A.
solani spore formation and infection) to three (conditions highly favorable
for spore formation and infection) (Gleason et al., 1995). The forecasting
program analyzes daily environmental data and maintains a record of the
(1) total of all S values (TS) since the beginning of the growing season,
(2) seven-day cumulative severity value (CS) calculated by totaling S values
for the past seven days, and (3) five-day cumulative rating value (CR) calculated by totaling R values for the past five days. The first early blight spray
application is recommended when TS reaches a critical level of 35 and the
plants are in the field for at least five weeks. Subsequent fungicide applications are scheduled when CS or CR equal or exceed prespecified critical
limits (Madden et al., 1978). The program was computerized for rapid and
accurate analyses. The FAST system has resulted in lower fungicide appli-
cation than is used in commercial fields, without sacrificing disease control

and crop yield (Gleason et al., 1995). The FAST system has been modified
during the past two decades because of feedback from the field and finetuned. A modified FAST program called TOM-CAST (Tomato Forecaster)
has been developed (Gleason et al., 1995). In this system, the disease severity values (DSVs) are calculated solely on the basis of the dew model of
FAST. Another departure from the FAST model is to sum DSVs, after the
first fungicide spray, from the date of the last spray rather than over a five- or
seven-day time window as in FAST (Gleason et al., 1995). TOMCAST can
be used, not only for early blight prediction, but also to advise growers when
to apply fungicides to control anthracnose and Septoria leaf spot. Use of the
forecasting model TOM-CAST results in lower fungicide application and
results in high quality fruit (Hong et al., 2000).
Apple Fire Blight Forecasting Models
An American predictive model, MARYBLYT, predicts the occurrence of
fire blight. The predictive criteria used for blossom blight are: (1) accumulation of at least 110 degree hours 18.3oC starting at full pink in apples;
(2) an average daily temperature of 16.6oC; (3) a wetting event with 0.25
mm rain on the day before or wetness of >6 hours; and (4) flowers must be
open with petals intact. All four conditions need to be fulfilled on the same
day for an infection event to occur. These predictions match observed fire
blight incidences (Gouk et al., 1999). This model has been used for the past
18 years in the United States and it accurately predicted the occurrence of
blossom blight in orchards.
Another predictive program, FireWork, implements MARYBLYT rules
using software written in Delphi 4 for Windows 95 and Windows NT. The
program is driven by weather data stored in the Orchard 2000 weather database. This program is accessible to farmers for orchard management decisions about antibiotic spray timing (Gouk et al., 1999). Several other fire
blight prediction models are available. Three fire blight predictive models,
MARYBLYT, Cougarblight (Smiths model), and the Mean Temperature
Line model, were compared to predict fire blight incidence in California.
The models accurately predicted low fire blight potential during the susceptible bloom period (Holtz et al., 1999). Rain events during this period were
associated with cold temperature, which reduced disease potential. Warm
rain (>16 C) favors disease incidence (Holtz et al., 1999).
An automated weather and fire blight model system was developed and
is currently being used by farmers in Pennsylvania. Simulated weather data
available for past, present, and future weather events are interfaced with disease models. The weather information is provided as both observed and
forecast for specific farm locations. In addition, the growers receive daily
fire blight alerts, which include a record of the fire blight infection risk for
the previous days in the month and a seven-day forecast of the fire blight
risk. The growers receive simulated weather reports and daily disease risk
alerts for fire blight via e-mail or fax. It was reported that the growers found
the simulated weather and fire blight alerts useful (Travis et al., 1999).
Billing (1980) developed a system in Europe to predict when outbreaks
of fire blight would likely be found in the field. The method is based on experimental studies of the growth rate of E. amylovora in vitro and historical
fire blight outbreaks in southeast England. This model determines the potential doubling of bacteria (PD) from daily maximum and minimum temperatures. The PD values are used to determine the duration of an incubation
period. The incubation period is completed and infection should be apparent in the field when the PD value accumulated from an infection day exceeds a certain threshold determined by temperatures and rainfall (Billing,
1980). This system was further modified and Billings Revised System
(BRS) and Billings Integrated System (BIS95) were developed (Billing,
1999). BIS95 aims to assess risks during the growing season, including low
and high incidence risks. For simplicity, degree day sums are used in this
BIS95 system. Following a warm period, wetting of flowers by dew is sufficient for infection. When temperatures are high ( 27C), blossom infection
may occur without wetting. Degree day sums above a temperature of 13.0
have provided good guidance on times when, following infection, early
signs of disease may be seen. The critical degree day sum for apple blossom
blight is suggested to be 47 (Billing, 1999). BIS95 provides rules based on
both weather data and field infection data. The efficacy of both MARYBLYT
and BIS95 systems were compared for their relative efficacy in prediction of
fire blight in Hungary and Turkey. Both the MARYBLYT and BIS95 systems were equally useful in providing guidance for obtaining daily results
about the progress of different infection events, including symptom development, and for making decisions on control. The Cougarblight Fire Blight
Risk Assessment Model was developed in the Northwest United States to
address the daily fire blight infection risk in orchards (Smith, 1999). Host
susceptibility, relative pathogen presence, and potential growth rate of bacterial colonies on stigma were all addressed prior to potential flower wetting. This model is successfully used by growers in the Pacific Northwest.
Citrus Postbloom Fruit Drop and Anthracnose Forecasting Model
The citrus postbloom fruit drop and anthracnose (Glomerella cingulata)
forecasting system was developed in Florida (Timmer and Zitko, 1993). The
most important parameters in disease prediction were the number of af-
fected blossoms already on the tree (inoculum availability) and rainfall during the previous five days. Timmer and Zitko (1993) developed the following equation to predict the disease 3 to 4 days in advance:
y = 7.5 + 1.28 (TD) + 0.44 (R
100)
where y = predicted percentage of flowers affected, TD = total number of

flowers affected on 20 trees, and R = rainfall total (mm) for the last five
days. Sprays should be applied when more than 20 percent disease is predicted. Model-based decisions on fungicide applications result in reduced
disease, large increases in fruit production, and elimination of unnecessary
sprays (Timmer and Zitko, 1996).
Grapevine Downy Mildew Forecasting Models
Several forecasting models have been developed to predict downy mildew (Plasmopara viticola) epidemic in grapevine. The prediction systems
were based on a detailed simulation of the disease cycle (Park et al., 1997),
simplified models of one or more components of the disease cycle that can
be used to predict a relative risk of disease development (Ellis et al., 1994),
or empirical models based on long-term climatic conditions, with little direct appraisal of the disease cycle (Vercesi et al., 1994). Some of these prediction systems (such as EnviroCaster) are sold commercially as electronic
predictive systems (Ellis et al., 1994). An electronic warning system based
on models for the infection of leaves of the American grape, Vitis lambrasca, production of sporangia by P. viticola in lesions, and sporangial survival has been found to be useful to plan strategies for control of the disease
(Madden et al., 2000).
Wheat Tan Spot Forecasting Model
De Wolf and Francl (2000) developed a model to predict wheat tan spot
(Pyrenophora tritici-repentis) epidemic. A leaf wetness period of more than
ten hours and precipitation of more than 3.4 mm are highly correlated with
the tan spot epidemic. Leaf wetness duration and precipitation predicted
validation cases of tan spot with about 72 percent accuracy in a sensitive
analysis of single independent variable disease forecasting models (De Wolf
and Francl, 2000). When the leaf wetness duration variable was withheld
from the disease-forecasting model, prediction accuracy on validation cases
decreased by 20 percent compared to the full model.
Wheat Septoria Forecasting Model

A Septoria forecasting model (Septoria-Timer) was developed (Verreet
et al., 2000). The Septoria-Timer is placed in the crop at the beginning of the
vegetation period. The equipment consists of a sensor for the detection of
leaf surface wetness and a datalogger. From the subsequent information, the
starting point for Septoria tritici infections is calculated on the basis of a
leaf wetness period of more than 98 percent. The result is then shown via an
easily read display. After a Septoria warning is given, the initial outbreak
has to be diagnosed and quantified. If both criteria for infection are fulfilled
(warning given by the Septoria-Timer and an initial infection 50 percent
disease incidence), a fungicide application is recommended (Verreet et al.,
2000).
Peanut Early and Late Leaf Spot Forecasting Models
A peanut early leaf spot disease (Cercospora arachidicola) forecasting
system (Early Leaf Spot Advisory) was developed in Georgia in 1966 using
the duration of relative humidity 95 percent and the minimum temperature
during the high humidity period (Jensen and Boyle, 1966). This system was
later computerized and designated as 81-ADV. This system has been used
successfully in Virginia. A second advisory system (89-ADV) was developed in Virginia and has replaced the 81 ADV. A survey showed that 94 percent of peanut growers in Virginia used the 89-ADV system (Phipps, 1993).
A predictive system using an EnviroCaster weather monitoring station and
microprocessor was developed for late leaf spot (Phaeoisariopsis personata). This model is based on the accumulation of hours of leaf wetness. Another model, AU-Pnuts advisory was developed by Auburn University in
Alabama in 1995 (Jacobi et al., 1995). The model was developed to schedule initial and subsequent fungicide applications for control of both early
and late leaf spots of peanut. The advisory uses the number of days with precipitation greater than 2.5 mm and National Weather Service precipitation
probabilities to predict periods favorable for development of early and late
leaf spot.
Strawberry Gray Mold Forecasting Models
Three forecasting systems for strawberry gray mold (Botrytis cinerea)
have been developed (Xu et al., 2000). The models were developed to relate
fungal inoculum and weather conditions to the incidence of flower infection; by inoculum only, by weather variables only, and by both inoculum
and weather variables. Models using weather variables only gave more ac-
curate predictions than models using inoculum only. Models using both
weather variables and inoculum gave the best predictions, but the improvement over the models based on weather variables only was small. Hence,
models based on weather variables (low day vapor pressure deficit and high
night temperature) will be highly useful in prediction of the disease (Xu
et al., 2000).
Pear Brown Spot Forecasting Model
A forecasting model (BSPcast) was developed for the prediction of pear
brown spot (Stemphylium vesicarium) (Llorente et al., 2000). This forecasting system is based on an empirical model. In this model, daily wetness duration (W) and mean air temperature during wetness periods (T) are used to
compute a daily disease severity (S) according to the following equation:
Log10(S)= 1.70962 + 0.0289T + 0.04943W +
0.00868TW 0.002362W2 0.000238T2W
Since the maximum daily disease severity predicted by the equation is
3.7942, relative daily infection risk (R) is calculated as:
R = S/3.7942
Therefore, R ranged from 0 to 1. The three-day cumulative daily infection
risk (CR) was computed by totaling R values for the past three days and was
used as an action threshold for spraying fungicides.
Mango Anthracnose Forecasting Models
Two predictive models were developed to predict mango anthracnose
(Colletotrichum gloeosporioides). Fitzell et al. (1984) studied the requirements of temperature and wetness duration for the production of dark
appressoria from conidia applied to detached young mango leaves under
laboratory conditions. They obtained the following equation:
Y = In P/q = 16.114 + 1.120T 0.0225T2 + 1.0862 In t
Where P = percentage of conidia forming dark appressoria, T = temperature
(C), t = wet period (h), and q = 100 P. The symbol In indicates natural logarithm. This system was built into a microprocessor-based forecasting system (Fitzell et al., 1984). Infection levels were estimated from temperature
and wetness duration following at least 0.2 mm of rain. If the model predicted that 10, 20, or 40 percent of conidia would form dark appressoria, it
would indicate that low, medium, or high levels of anthracnose infection, respectively, would occur (Fitzell et al., 1984). A similar system was developed by Dodd et al. (1991). This system differs from the previous model, in
that it was developed using combined data from fruit and leaf inoculations
and includes relative humidity in addition to wetness and temperature
(Arauz, 2000).
Corn Stewarts Wilt Forecasting Model
A predictive system to forecast corn Stewarts disease (bacterial wilt)
was developed in the United States. Epidemic development of the disease is
correlated with winter temperatures. Epidemics develop following relatively mild temperatures. A winter temperature index is calculated by adding the mean temperatures (expressed as F) for December, January, and
February. When the winter temperature index is less than 90, it is predicted
that there will not be any disease incidence and there is no need for application of insecticides. If the temperature index is 90 to 100, the disease severity may be intermediate, and if the temperature index is more than 100, the
disease severity will be severe. This predictive system works well in various
areas of the United States (Pataky et al., 2000).
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CROP DISEASE MANAGEMENT
17
Biological
Biological
ControlMicrobial
ControlMicrobial Pesticides
Pesticides
Biological control using microbial pesticides has become important in
recent years. Introduced microbial biocontrol agents (as opposed to a natural population of microbes) are called microbial pesticides according to
the Environmental Protection Agency (EPA) (Harman, 2000). Several microbial pesticides are now commercially available. Fungal, bacterial, and
viral biocontrol agents are available. They effectively control fungal, bacterial, and viral diseases. Modes of action of these biocontrol agents are described. Conditions favorable for effective action of them in controlling diseases are also discussed.
TRICHODERMA
Trichoderma species are the most important biocontrol agents. Many
have been developed as commercial products. In 1999, retail sales of a product (Topshield and Rootshield) based on a single strain of T. harzianum,
strain T-22, totaled around 3 million dollars in the United States (Harman,
2000). Commercial production of Trichoderma has been reported from
France, New Zealand, Sweden, Poland, Denmark, Russia, Israel, Bulgaria,
China, and India. There are many constraints in developing Trichoderma as
biocontrol agents. Trichoderma colonizes in the spermosphere effectively,
but they normally do not survive well in the rhizosphere. Trichoderma spp.
achieve only transitory localized dominance of the rhizosphere, and these
are active in only some soils and seasons (Deacon, 1994). Hence, Trichoderma species are likely to be effective for seed and seedling diseases, but
not against diseases of a mature crop. However, crop losses will be greater
when mature crops are affected, and seed and seedling diseases can be effectively controlled by seed treatment with chemicals at a very low cost.
Under such conditions, the use of Trichoderma spp. will be limited. Another
important constraint is that Trichoderma spores are quiescent and inactive
in soil. Hence, Trichoderma strains cannot be added as spores. It may be
easier to mass multiply fungi in the form of spores.
Several technologies were recently developed to make use of Trichoderma in the control of soil-borne diseases of crops at different maturity
stages. Trichoderma harzianum strain T-22 with rhizosphere competence
was developed by protoplast fusion technology (Harman, 2000). Rhizosphere competence is defined as the ability of a microorganism to grow and
function in the developing rhizosphere. Strains that were fused were T-95 of
T. harzianum, a rhizosphere competent mutant produced from strain T-12.
T-12 was more capable of competing with spermosphere bacteria than T-95
under iron-limiting conditions; both were strong biocontrol agents. Some
strains of Trichoderma, such as the strain T39, can induce systemic resistance, and such strains can induce resistance against diseases at any stage of
the crop (De Meyer et al., 1998). Technology to apply Trichoderma in the
form of actively growing germlings instead of spores was developed to obtain an active population of Trichoderma in soil to control soil-borne pathogens.
Formulations of Trichoderma
Liquid media based on molasses and molasses yeast have been used
widely for the production of Trichoderma. The addition of complex organic
materials, such as V8 juice, yeast extract, or protease peptone, increased
conidial production in T. harzianum. The addition of osmotica such as polyethylene glycol improved conidial production of T. harzianum and resistance of conidia to desiccation (Whipps, 1996). Trichoderma harzianum
has been produced in diatomaceous earth granules impregnated with 10 percent molasses. Spores, cells, or biomass are concentrated directly from liquid media by centrifugation and filtration. Biomass may be dried, milled,
and incorporated into a range of dusts, alginate granules, pellets or prills,
wettable powders, emulsifiable liquids or gels. Talc formulations, kaolinbased microgranules, and alginate pellet, prill, or granule formulations are
available.
Conidia of Trichoderma are added to a bran-sand mixture, and after one
to three days of incubation, this germling preparation is added to soil where
colony-forming units of the antagonists continue to increase. This method
provides a means of achieving an active population of antagonists in the
soil. A medium supplemented with ground corn cobs was developed for
applying T. koningi in the field (Latunde-Dada, 1993). Alternatively, a fermenter biomass of Gliocladium and Trichoderma spp. was added to a vermiculite-bran mixture moistened with 0.05 M HCl. After drying, the preparation can be remoistened with 0.05 M HCl and germlings produced as
before (Lewis et al., 1991).
Seed-coating formulations have also been developed. A liquid coating

formulation comprises a suspension of aqueous binder (pelgel or polyox
N-10), finely ground solid particulate matter (Agro-Lig or muck soil), and
the biocontrol agent (Taylor et al., 1991). This is sprayed onto seeds in a
tumbling drum. Agro-Lig has chemical and physical characteristics favorable for the growth of Trichoderma. This type of formulation was very effective in the control of damping-off of cucumber caused by Pythium (Taylor et al., 1991). Adding compounds to the seed coating that specifically
enhance growth of Trichoderma is highly beneficial. Inclusion of specific
polysaccharides and polyhydroxy alcohols improves biocontrol activity of
Trichoderma (Nelson et al., 1988).
Method of Application of Trichoderma
Mathre et al. (1999) stated that nearly all commercialized microorganisms rely upon application of the antagonist directly and precisely to the infection court when and where needed. Seed treatment is the most effective
method (Mathre et al., 1999). Seed-coating formulations will be useful.
Seed priming is also recommended. Seed priming is the process in which
hydration of the seed is controlled to a level that permits pregerminative
metabolic activity to take place without emergence of the radicle. Two priming systems are available. Osmopriming utilizes aerated aqueous solutions
of salts or polyethylene glycol, generating osmotic potential in the primary
solution. Solid matrix priming (SMP) involves the use of moist, porous
solid materials, such as powdered coal or peat, generating matric potential.
Combining SMP with Trichoderma spp. for control of seedling diseases has
been used successfully on a wide range of plants (Harman et al., 1989).
Actively growing germling populations can be applied to soil. Trichoderma can be applied as granules or as a drench. A single application of
T. harzianum T-22 as RootShield granules in a greenhouse provided protection of a tomato crop against Fusarium crown and root rot of the mature
crop. An in-furrow drench was more effective in both root colonization and
disease control than a seed treatment (Harman, 2000).
In crops that are transplanted, the granules can be applied in the nursery.
Tomatoes were grown in a potting mix containing the granular formulation
of T. harzianum T-22, which permitted roots to become colonized, and then
transplanted to the field. This treatment reduced Fusarium crown and root
rot at the harvest of mature fruit (Datnoff et al., 1995). Trichoderma can also
be applied as a spray. Trichoderma harzianum T-22 is effective in the control of fruit and foliar diseases when applied as a spray to these plant parts.
T-22 should be applied at least once every 10 days when disease pressure is
high, because it cannot extensively grow on and colonize newly formed leaf
tissues. The fungus colonizes grape or strawberry flowers and immature
fruits (Harman, 2000). Diseases controlled by foliar spray include powdery
mildews of Catharanthus and pumpkins, Botrytis cinerea on strawberry,
downy mildew of snapdragons, and turf-grass pathogens such as Rhizoctonia solani and Pythium spp. (Harman, 2000).
Bumble bees (Bombus impatiens) and honey bees (Apis mellifera) have
been used to deliver Trichoderma to the flowers of crop plants. Bees exiting
the hive pass through a device that requires them to come into contact with
Trichoderma products containing these spores. They subsequently deliver
substantial amounts of Trichoderma harzianum T-22 or similar fungi to the
strawberry or other flowers. This method of delivery was more effective
than spray applications for control of B. cinerea and has proven effective
over several years and trials as standard chemical applications (Kovach
et al., 2000; Harman, 2000).
Time of application of Trichoderma is also important. Trichoderma can
be overwhelmed by heavy disease pressure. Therefore, T. harzianum may
be used strictly as a preventative measure; it cannot cure infections. Trichoderma is less effective against systemic diseases than against more superficial ones. It cannot control existing diseases, and so a good systemic fungicide must be used if diseases already exist. In conditions of high or very
high disease pressure, T-22 should be used as part of an integrated chemicalbiological system. A combination of chemical treatment with Trichoderma
will be highly effective in the control of diseases. A tank mix with chemical
fungicides or an alternating spray with chemical fungicides is the ideal
method of application of Trichoderma (Harman, 2000). A combination of
ozone fumigation and T. harzianum treatment was on par with the standard
methyl bromide treatment, and the combination was significantly better
than either T. harzianum alone or ozone fumigation alone in control of
strawberry root diseases (Harman, 2000).
A single strain of Trichoderma may not be sufficient to be effective under
all conditions and against all diseases. Mathre et al. (1999) suggested that
almost invariably, a different agent might be needed for each disease. Cook
(1993) stated that biological control is widely recognized as being highly
disease-specific. He advocated an approach to biological control that uses
mixtures of numerous agents for each disease. A mixture of Trichoderma
spp. has been developed as commercial formulations. T. harzianum + T.
polysporum (BinabT) and T. harzianum + T. viride (Trichodowels) are
the important complex products (Whipps, 1996). Trichoderma has been
combined also with other biocontrol agents. The combination of T. harzianum T-22 and the mycorrhizal fungus Glomus intradices was more effective than either organism alone (Datnoff et al., 1995). There are also reports
that a single strain of Trichoderma may be capable of controlling diverse

pathogens under diverse conditions (Chet, 1987; Harman, 2000).
Diseases Controlled by Trichoderma
Trichoderma has been reported to control Rhizoctonia, Fusarium, Phytophthora, and Pythium diseases in many crops, tomato root and crown rot,
pumpkin and Catharanthus powdery mildews, gray mold (Botrytis cinerea)
of strawberry, root rots of several crops caused by Macrophomina phaseolina, wheat take-all caused by Gaeumannomyces graminis var. tritici, Sclerotinia and Verticillium diseases of fruit trees, Armillaria mellea infection in
trees, Dutch elm disease (Ceratocystis ulmi), Chestnut blight (Endothia
parasitica), silver leaf disease of trees (Chondrostereum purpureum), and
stem and root rot of pine (Heterobasidion annosum) (Harman et al., 1989;
Nelson et al., 1988; Maplestone et al., 1991; Whipps, 1992, 1996; Datnoff
et al., 1995; Nemec et al., 1996; De Meyer et al., 1998; Elad et al., 1999;
Howell et al., 1999; Burns and Benson, 2000; Harman, 2000).
Mycoparasitism of Trichoderma
Trichoderma spp. may control diseases caused by various fungal pathogens by their various types of actions. Their modes of action include mycoparasitism, antibiosis, induced resistance, competition for nutrients or space,
and inactivation of the pathogens enzymes. Mycoparasitism involves tropic
growth of the biocontrol agent toward the target fungi, lectin-mediated coiling of attachment of Trichoderma hyphae to the pathogen, and finally attack
and dissolution of the target fungal cell wall by activity of enzymes, which
may be associated with physical penetration of the cell wall. More than 20
separate genes may be involved in mycoparasitism. Trichoderma produces
ten different chitinases and several -1,3-glucanases and proteases. The importance of chitinases produced by the antagonist has been demonstrated in
several ways. A 42-kDa endochitinase is induced before T. harzianum comes into contact with Botrytis cinerea (Zeilinger et al., 1999). A strain of T.
harzianum deficient in the ability to produce endochitinase had reduced
ability to control B. cinerea (Woo et al., 1999). Endochitinase was disrupted
or overproduced in T. virens, and the resulting strains were found to have decreased or increased biocontrol activity, respectively (Back et al., 1999).
Expression of endochitinase from T. harzianum in transgenic apple increases resistance to apple scab (Bolar et al., 2000). The endochitinase gene
from Trichoderma confers resistance in many other transgenic plants
(Lorito et al., 1998). Contradicting reports state that chitinase may not be involved in an antagonistic action of Trichoderma. The activity of endochitinase was disrupted or overproduced in T. harzianum, but these changes
had no effect on its biocontrol ability against Rhizoctonia solani or Sclerotium rolfsii (Carsolio et al., 1999). A strain of T. harzianum deficient in the
ability to produce endochitinase had increased ability to control R. solani
(Woo et al., 1999). These results suggest that other gene products may also
be involved in the action of Trichoderma.
Antibiotic Production by Trichoderma
Forty-three antibiotic substances were reported to be produced by Trichoderma spp. (Sivasithamparam and Ghisalberti, 1998). Of these, alkyl
pyrones, isonitriles, polyketides, peptaibols, diketopiperazines, sesquiterpenes, and steroids are important and found to be associated with biocontrol
activity of Trichoderma spp. Mutation to eliminate production of specific
antibiotics is associated, in some strains, with a loss of activity against particular pathogens (Howell, 1998).
Induced Resistance by Trichoderma
Some Trichoderma spp. induce systemic resistance against pathogens.
Trichoderma harzianum induces systemic resistance against powdery mildews (Elad et al., 1999), B. cinerea infections (De Meyer et al., 1998), and
root rot of cotton (Howell et al., 1999). Trichoderma products (BINABTFWP and BINABT vector) induced systemic acquired resistance in strawberry against Botrytis cinerea (Ricard and Jorgensen, 2000). Different
elicitors, including xylanase, have been isolated from Trichoderma and they
trigger the synthesis of various defense compounds, including phytoalexins
(Calderon et al., 1993). Trichoderma induces the synthesis of phytoalexins
involved in disease resistance in cotton (Howell et al., 1999), modifies and
strengthens plant cell walls in cucumber (Yedidia et al., 1999), and increases activities of chitinase and peroxidase in cucumber tissues (Yedidia
et al., 1999). Cucumber roots treated with T. harzianum exhibited higher activities of chitinase, -1,3-glucanase, and peroxidase (Yedidia et al., 2000).
For more information on the role of peroxidases, -1,3-glucanases and
chitinases in conferring disease resistance, see Chapter 34.
Competition for Space or Nutrients by Trichoderma

Trichoderma may compete for space or nutrients with pathogens and
suppress their development. Botrytis cinerea conidia require external nutrients for germination and infection. When conidia of T. harzianum T39 were
applied to leaves, germination of conidia of the pathogen was slowed, an effect attributed in part to competition (Elad et al., 1999).
Inactivation of the Pathogens Enzymes by Trichoderma
B. cinerea depends upon production of pectolytic, cutinolytic, and cellulolytic enzymes to infect living plants. However, conidia of two strains of T.
harzianum (T39 and NCIM 1185), when applied to the leaves, produce a
serine protease that is capable of degrading the pathogens plant cell wall
degrading enzymes and thereby reducing the ability of the pathogen to infect the plant (Elad and Kapat, 1999). The biocontrol activity of T39 could
be enhanced by adding additional quantities of its protease. Several protease
inhibitors reduced the biocontrol activity of T39 (Elad and Kapat, 1999).
PSEUDOMONAS SPECIES
Pseudomonas spp. are the important group of biocontrol agents that have
been developed as commercial products. Fluorescent pseudomonads form
the major bacterial group surviving in the rhizosphere of crop plants. They
are also known as plant growth promoting rhizobacteria (PGPR) because
they promote plant growth by secreting auxins, gibberellins, and cytokinins
(Vidhyasekaran, 1998). These pseudomonads survive in soil, rhizoplane,
phylloplane, pistils, nectarines, and fruits of the plants. Pseudomonas fluorescens, P. putida, and P. cepacia (Burkholderia cepacia) are important
Pseudomonas species and are known as highly effective biocontrol agents.
They have been reported to control soil-borne, seed-borne, and air-borne
fungal, bacterial, and viral pathogens.
Diseases Controlled by Pseudomonas
The following is a list of diseases that have been reported to be controlled
by saprophytic pseudomonads (Levy et al., 1998; Alstrom, 1991; Wei et al.,
1991; Liu et al., 1992, 1995a,b; Wilson and Lindow, 1993; Maurhofer,
Scherer, et al., 1994; Tosi and Zazzerini, 1994; Hoffland et al., 1995; Leeman et al., 1995b; Raaijmakers et al., 1995; Vidhyasekaran and Muthamilan, 1995, 1999; Benhamou et al., 1996; MPiga et al., 1997; Vidhyase-
karan, 1998, 2001; Vidhyasekaran, Rabindran, et al., 1997; Vidhyasekaran,

Sethuraman, et al., 1997; Vidhyasekaran et al., 2000):
Applegray mold (Botrytis cinerea)
Beanhalo blight (Pseudomonas savastanoi pv. phaseolicola), root
rot (Sclerotium rolfsii)
Chickpeawilt (Fusarium oxysporum f. sp. ciceris)
Cottonbacterial blight (Xanthomonas axonopodis pv.
malvacearum), Fusarium wilt (Fusarium oxysporum
Schlechtend.: Fr. f. sp. vasinfectum), seedling blight (Rhizoctonia
solani), and seedling rot (Pythium ultimum)
Cucumberwilt (Fusarium oxysporum f. sp. cucumerinum), damping-off (Pythium aphanidermatum), anthracnose (Colletotrichum
orbiculare), angular leaf spot (Pseudomonas syringae pv.
lachrymans), and mosaic (Cucumber mosaic virus)
Peawilt (Fusarium oxysporum f. sp. pisi), root rot (Aphanomyces
euteiches), and damping-off (Pythium ultimum)
Peanutlate leaf spot (Phaeoisariopsis personata), stem rot (southern blight) (Sclerotium rolfsii), and rust (Puccinia arachidis)
Pearfire blight (Erwinia amylovora)
Pigeonpeawilt (Fusarium oxysporum f. sp. udum)
Potatobacterial wilt and brown rot (Ralstonia solanacearum),
black leg and soft rot (Erwinia carotovora)
Radishwilt (Fusarium oxysporum f. sp. raphani)
Riceblast (Pyricularia oryzae), sheath blight (Rhizoctonia solani),
bacterial blight (Xanthomonas oryzae pv. oryzae), sheath rot
(Sarocladium oryzae), and tungro (Rice tungro virus) diseases
Safflowerrust (Puccinia carthami)
Sugar beetleaf spot (Phoma betae), damping-off (Pythium
ultimum), and root rot (Aphanomyces euteiches)
Tobaccoblack root rot (Thielaviopsis basicola), necrosis (Tobacco
necrosis virus)
Tomatowilt (Fusarium oxysporum f. sp. lycopersici)
Wheattake-all (Gaeumannomyces graminis var. tritici), leaf
(brown) rust (Puccinia triticina=Puccinia recondita),
and Septoria speckled leaf blotch (Septoria tritici)
Induction of Systemic Resistance by Pseudomonas Spp.
Several rhizobacterial strains were shown to elevate plant resistance
against several pathogens. Strains of Pseudomonas fluorescens, P. putida,
and P. aeruginosa induced systemic resistance (ISR) in rice (Ohno et al.,

1992; Vidhyasekaran, Rabindran, et al., 1997; Vidhyasekaran et al., 2000),
cucumber (Liu et al., 1995a,b; Meyer et al., 1992; Raupach, 1996), radish
(Leeman et al., 1995b), tobacco (Maurhofer et al., 1994a), tomato (Van
Wees et al., 1997; MPiga et al., 1997), bean (Meier et al., 1993), pea
(Benhamou et al., 1996), carnation (Van Peer and Schippers, 1992), and
Arabidopsis (Van Wees et al., 1997).
Rhizobacterial strains have been shown to induce different defense genes
in plants. Maurhofer, Hase, et al. (1994) showed that ISR by P. fluorescens
strain CHA0 in tobacco was associated with accumulation of various
pathogenesis-related (PR) proteins (PR-1, PR-2, and PR-3 proteins). Inoculation of bean leaves with cells of P. fluorescens induced the accumulation
of transcripts for chalcone synthase (CHS), chitinase, and lipoxygenase
(Meier et al., 1993). Increase in peroxidase activity as well as an increase in
the level of mRNAs encoding for phenylalanine ammonia-lyase (PAL) and
CHS could be recorded in the early stages of the interaction between roots
and various bacterial endophytes (Zdor and Anderson, 1992). Treatment
with P. fluorescens causes increases in activities of peroxidase, lysozyme,
and PAL in tobacco (Schneider and Ullrich, 1994). Van Peer et al. (1991)
showed massive accumulation of phytoalexins in carnation roots colonized
by the rhizobacterial strains after pathogen challenge. MPiga et al. (1997)
reported that P. fluorescens strain 63-28 induced accumulation of an electron-dense material in epidermal and outer cortical cells and coating of most
intercellular spaces with similar substances in tomato. This aggregated material appeared to be mainly composed of phenolic compounds, especially
phenols containing O-hydroxy groups. The deposition of -1,3-glucans
(callose) was also observed in host cell walls. Chitinases were also induced
(MPiga et al., 1997). Benhamou et al. (1996) demonstrated that pea root
bacterization with P. fluorescens triggered a set of defense reactions that resulted in the elaboration of permeability barriers. Increases in plant lignification, phytoalexins, various lytic enzymes, and other PR proteins have
been observed upon treatment of plants with different specific strains of
rhizobacteria (Albert and Anderson, 1987; Frommel et al., 1991; Kloepper
et al., 1993; Sayler et al., 1994; MPiga et al., 1997; Vidhyasekaran et al.,
2000).
Studies on the mode of induction of defense genes by the rhizobacterial
strains reveal that different bacterial cell wall components may act as
elicitors of induction of the signal transduction system. Lipopolysaccharides (LPS) of P. fluorescens strain WCS417r act as elicitors and induce resistance against different diseases (Duijff et al., 1997). LPS and LPS-containing cell wall preparations of P. fluorescens WCS417r are as effective as
living WCS417r bacteria in inducing ISR in radish (Leeman et al., 1995a).
The O-antigenic side chain of the outer membrane LPS of the strain
WCS417r appears to be the main determinant for induction of ISR in radish
and carnation (Van Peer and Schippers, 1992; Leeman et al., 1995a; Duijff
et al., 1997).
Leeman et al. (1996) demonstrated that the siderophore of WCS374r can
act as an elicitor of ISR in radish. Pseudomonas fluorescens strain CHA0 induced systemic resistance in tobacco. A siderophore (pyoverdine)-deficient
derivative of this strain no longer induced ISR (Maurhofer, Hase, et al., 1994).
Leeman et al. (1996) reported that the purified siderophore, pseudobactin,
from P. fluorescens strain WCS374 induced ISR in radish. However, a
pseudobactin-deficient, P. fluorescens 374PSB, retained ISR-inducing activity. These results suggest that siderophore production by this strain was
only partially responsible for the induction of systemic resistance in radish.
Pyochelin, a siderophore, is produced by several rhizobacteria. Salicylic
acid is a precursor of pyochelin synthesis (Leeman et al., 1996). Several
genera of bacteria, including fluorescent pseudomonads, are known to synthesize salicylic acid (Dowling and OGarra, 1994). Pseudomonas fluorescens strain CHA0 produces salicylic acid (Meyer et al., 1992). Leeman
et al. (1996) reported that P. fluorescens strain WCS374 produced salicylic
acid in quantities that were iron dose-dependent. Pseudomonas fluorescens
strain WCS417r has the capacity to produce salicylic acid (Leeman et al.,
1996). Salicylic acid was responsible for the induction of resistance in radish (Leeman et al., 1996). Meyer et al. (1992) reported that salicylic acid
itself might function as an endogenous siderophore. Leeman et al. (1996)
reported that rhizobacteria-mediated ISR is affected by iron concentration.
Salicylic acid production is iron (Fe3+) regulated (Leeman et al., 1996). Salicylic acid production is promoted by low iron concentrations. Increasing
ferric iron concentrations in vitro reduced salicylic acid production below
detectable limits by bacteria (Meyer et al., 1992). When ferric iron was applied as a soil drench, ferric iron concentration increased in planta; but it
significantly reduced the level of ISR observed in cucumber. This suggests
that salicylic acid may not be involved in ISR; but some other siderophores
mediated by iron may be involved in induction of ISR in cucumber.
Massive accumulation of phytoalexins could be detected in roots of carnation plants treated with P. fluorescens only after challenge inoculation
with pathogen (Van Peer et al., 1991). The induction of phenolics and
callose in tomato by P. fluorescens strain 63-28 was substantially amplified
upon infection with the pathogen (MPiga et al., 1997). These results suggest that the rhizobacterial strains may be capable of evoking transcriptional
activation of plant defense genes, the expression of which may be subsequently latent until the plant perceives signals originating from contact with
the pathogen. It is also possible that besides the rhizobacterial signal mole-
cules, pathogens signal molecules may also be involved in the induction of

ISR. Induction of H2O2 production due to P. fluorescens treatment in plants
has also been reported (Jakobek and Lindgren, 1993), and H2O2 is known as
a second messenger that triggers the synthesis of defense chemicals.
Production of Antibiotics by Pseudomonas
Pseudomonas spp. produce antibiotics that have been assigned a role in
disease control. Pseudomonas fluorescens CHA0 produces 2,4-diacetylphloroglucinol, pyoluteorin, and pyrrolnitrin (Voisard, 1994; Maurhofer,
Sacherer, et al., 1994). Pseudomonas fluorescens 2-79 produces phenazinecarboxylic acid, pyoverdine, and anthranilic acid, with biocontrol activity
mainly due to phenazine production (Hamdan et al., 1991). Pseudomonas
fluorescens Pf-5 produces 2,4-diacetylphloroglucinol, pyrrolnitrin, and pyoluteorin, with pyoluteorin being the most effective against Pythium ultimum
(Loper et al., 1994). Pseudomonas fluorescens DR54 produces the antibiotic viscosinamide (Thrane et al., 1999).
The importance of antibiotics in the biocontrol activity of Pseudomonas
spp. was demonstrated by several studies. Biocontrol activity could be increased by increasing the production of antibiotics. Phenazine production
could be increased by introducing extra copies of biosynthetic or activator
genes. This increased the biocontrol activity of the Pseudomonas strain
(Thomashow and Pierson, 1991). Additional evidence for a role of antibiotics in biocontrol by Pseudomonas spp. was obtained by heterologous expression of complementary genes in strains that naturally do not produce the
antibiotic, thus increasing their biocontrol activity (Hara et al., 1994) or by
use of Tn5 mutagenesis to inactivate specific genes and affect pathways of
antibiotic production (Schnider, Keel, Voisard, et al., 1995). In situ detection
of antibiotics in the rhizosphere of plants treated with antibiotic-producing
strains was demonstrated for phenazine-carboxylic acid (Thomashow et al.,
1990), 2,4-diacetylphloroglucinol (Maurhofer et al., 1995), pyrrolnitrin
(Kempf et al., 1994), and pyoluteorin (Maurhofer et al., 1995). In situ expression of the genes required for the synthesis of pyoluteorin, phenazines,
and Oomycin A could be demonstrated by fusing reporter genes such as
those encoding -galactosidae or ice-nucleating activity to promoters of
genes encoding for antibiotics (Loper and Lindow, 1994; Kraus and Loper,
1995).
A two-component regulatory system for antibiotic production in Pseudomonas spp. was reported. The system is based on two protein components,
an environmental sensor and a cytoplasmic regulator or global activator
(GacA) that mediates changes in response to sensor signals. A response reg-
ulator gene, gacA, was identified in P. fluorescens CHA0. Strains with a mutation of this gene lost the ability to produce pyoluteorin and 2,4-diacetylphloroglucinol (Laville et al., 1992). A lemA-like apd gene was found in P.
fluorescens Pf-5. Strains with a mutation in this region failed to produce
pyoluteorin and pyrrolnitrin, and lost the ability to inhibit Rhizoctonia
solani in culture (Corbell and Loper, 1995). Antibiotic production in Pseudomonas spp. may be further controlled by the action of housekeeping
sigma factors encoded by the rpoS or rpoD genes (Schnider, Keel, Blumer,
et al., 1995).
Role of HCN, Siderophore, and -1,3-Glucanase Produced
by Pseudomonas
Pseudomonas spp. produce hydrocyanic acid (HCN), which was shown
to be important in biocontrol activity (Laville et al., 1992; Voisard et al.,
1994; Loper et al., 1994; Corbell and Loper, 1995). The importance of a
Pseudomonas-produced pyoverdine siderophore in biocontrol activity was
demonstrated (Hamdan et al., 1991; Maurhofer, Hase, et al., 1994; Voisard
et al., 1994; Loper et al., 1994; Kraus and Loper, 1995). -1,3-Glucanase
may be involved in biocontrol activity of Pseudomonas (Burkholderia)
cepacia (Fridlender et al., 1993).
Factors Involved in Biocontrol Efficacy of Pseudomonas
Several factors determine the efficacy of Pseudomonas in controlling
crop diseases in the field. Effective strain selection is important (Vidhyasekaran, 1998). The antagonist inoculum dose determines the efficacy of the
antagonist in controlling diseases. A threshold population density of the fluorescent pseudomonad strains of approximately 105 cfu per g of root is required for significant suppression of fusarium wilt of radish. When rhizosphere population densities of the strains dropped below this threshold
level, the efficacy of these strains to suppress the fusarium wilt was almost
lost (Raaijmakers et al., 1995). Similar results were obtained in the control
of rice sheath blight by P. fluorescens strain Pf1 (Vidhyasekaran and Muthamilan, 1999) and P. fluorescens PFALR2 (Rabindran and Vidhyasekaran,
1996). The efficacy of fluorescent pseudomonads is affected drastically by
increasing disease pressure. Significant wilt disease suppression in radish
by fluorescent pseudomonads was observed when the disease incidence in
the control (untreated) field was less than 80 percent and the antagonists did
not suppress fusarium wilt when disease incidence exceeded 80 percent in
the control field (Raaijmakers et al., 1995). The antagonists may be highly
useful in moderately resistant varieties rather than highly susceptible varieties (Leeman et al., 1995b). It was suggested that the antagonists should be
integrated with chemical fungicides, with the two being applied alternatively (Vidhyasekaran, 1998).
The method of application also determines the efficacy of pseudomonads. Seed treatment with these antagonists appears to be very effective.
These bacteria establish well in the rhizosphere when introduced through
seed treatment (Vidhyasekaran, Sethuraman, et al., 1997; Vidhyasekaran
and Muthamilan, 1999). Seed treatment of peas with Pseudomonas aureofaciens (P. chlororaphis) protects pea plants against R. solani (Koch et al.,
1998). Biopriming of treated seeds increases the population of the antagonists in seed and effectively controls diseases (Callan et al., 1990; Vidhyasekaran and Muthamilan, 1995). Soil application was shown to be effective in
control of soilborne diseases (Hagedorn et al., 1993). Root dip treatment for
transplanted crops can be highly useful. A root dip into suspensions of P.
aureofaciens protects strawberry against Phytophthora fragariae var. fragariae (Koch et al., 1998). Several workers have successfully used foliar
spray of these antagonists to control foliar diseases (Mew and Rosales,
1986; Vidhyasekaran, Rabindran, et al., 1997).
Pseudomonads should be used as powder formulations only. Ten-day-old
bacterial cultures in a liquid nutrient medium are ineffective in controlling
diseases. Several formulations with different carrier materials were developed for application in the field. Peat- and talc-based formulations are commonly used (Hagedorn et al., 1993; Hofte et al., 1991; Vidhyasekaran,
Sethuraman, et al., 1997). Granular preparations of P. aureofaciens UKM
B-111 based on clay minerals show high survival, preservation of antagonistic activity, and stability of composition during long-term storage (Kurdish
et al., 1999). Vermiculite-based formulation of P. putida is effective in controlling Fusarium oxysporum f. sp. cucurbitacearum infection in cucumber
(Amer and Utkhede, 2000).
OTHER ORGANISMS
Gliocladium
The fungus Gliocladium catenulatum (Primastop) was developed as a
biofungicide. It was registered in the United States by Kemira Agro OY. The
same product will be registered as PreStop in Europe (Niemi and Lahdenpera, 2000). This product has proven effective in the control of damping-off
of vegetables, herb, and ornamental seedlings, root and stem rot diseases in
vegetables and ornamentals, Didymella in cucumber and tomato, and gray
mold in ornamentals. It is effective against various soilborne diseases

caused by Pythium, Phytophthora, and Rhizoctonia, foliar diseases caused
by Didymella, Alternaria, and Botrytis, and storage diseases caused by
Helminthosporium and Rhizoctonia (Niemi and Lahdenpera, 2000).
Pythium Oligandrum
Pythium oligandrum was developed as a commercial product named
Polygandron. The addition of P. oligandrum zoospores to soil reduced the
ability of sclerotia of Sclerotinia sclerotiorum to germinate. Pythium oligandrum reduces the survival of S. sclerotiorum present naturally in soils
through mycoparasitic activity (Madsen and Neergaard, 1999). The cellwall-degrading enzymes N-acetyl- -D-glucosaminidase, endo-chitinase,
-glucanase, -glucosidase, cellobiohydrolase, and protease were detected
in culture filtrates of P. oligandrum cultivated with S. sclerotiorum (Madsen
and Neergaard, 1999). P. oligandrum may also act by inducing resistance in
plants. A low molecular weight protein termed oligandrin was obtained
from culture filtrates of P. oligandrum (Picard et al., 2000). This protein induces plant defense reactions that help restrict stem cell invasion by Phytophthora nicotianae var. parasitica. Oligandrin is similar to elicitins.
Oligandrin-treated plants show reduced disease incidence in tomato caused
by P. nicotianae var. parasitica (Picard et al., 2000).
Verticillium Lecanii
Verticillium lecanii is a promising biocontrol agent of rusts and powdery
mildews that effectively controls cucumber powdery mildew caused by
Spherotheca fuliginea (Askary et al., 1998). Verticillium lecanii grows over
a wide temperature range, and a water film, or at least high humidity, is required for conidial germination. Thus, water is an important environmental
factor in the control of powdery mildews on cucumber and rose by V. lecanii
(Verhaar et al., 1996). The development of V. lecanii in pustules of Puccinia
striiformis was best at 95 to 100 percent relative humidity (RH), whereas no
development was observed at 80 percent RH (Mendgen, 1981). This suggests that high humidity is required for effective control of the pathogen by
V. lecanii. Verticillium lecanii produces antibiotics and hydrolytic enzymes
and they may be involved in the parasitism of V. lecanii on Penicillium
digitatum (Benhamou and Brodeur, 2000).
Yeasts
The yeast Torulopsis candida (= Candida famata) effectively controls
Penicillium digitatum infection on citrus fruits (Arras et al., 1999). Another
yeast, Debaryomyces hansenii, reduces Penicillium digitatum decay on orange fruits. The yeast elicited production of phytoalexins, scopoletin, and
scoparone, and did not produce toxic substances against the pathogens,
Penicillium digitatum and Botrytis cinerea. This suggests that the yeast may
reduce the fungal infection by activating hosts defense mechanisms (Arras
and Arru, 1999). The yeasts Candida saitoana and C. oleophila control
postharvest diseases of apple and citrus fruits (El-Ghaouth et al., 2000). The
yeast (Candida guilliermondii) suspension, when sprayed two to five times
at 7 to 10 day intervals, reduces decay caused by B. cinerea in both table
grapes (cultivars Thomson Seedless and Superior Seedless) and wine grapes
(cultivar Sauvignon blane), and rots caused by Aspergillus niger in wine
grapes (Zhavi et al., 2000). Another yeast, Pichia membranefaciens, controls storage rot of nectarine fruits caused by Rhizopus stolonifer (Fan and
Tian, 2000).
Aureobasidium
A cosmopolitan yeastlike fungus, Aureobasidium pullulans, colonizes
leaf surfaces and is a potential biocontrol agent for plant pathogens. It controls Botrytis cinerea on apples, Penicillium digitatum on grapefruits, B.
cinerea, Rhizopus stolonifer, and Aspergillus niger on table grapes and
B. cinerea and R. stolonifer on cherry tomatoes (Schena et al., 1999). Preharvest application of Aureobasidium pullulans isolate L47 on table grapes
results in a significant reduction of postharvest rot caused by B. cinerea
(Schena et al., 1999). Aureobasidium pullulans controlled apple decay
caused by B. cinerea and Penicillium expansum. The yeastlike fungus induced a transient increase in -1,3-glucanase, chitinase, and peroxidase activities in apple tissues, and all three enzymes are involved in host defense
mechanisms (Ippolito et al., 2000; Castoria et al., 2001). The biocontrol
agent also has capacity to out-compete pathogens for nutrients and space
(Ippolito et al., 2000; Castoria et al., 2001).
Penicillium
Talaromyces flavus (anamorph Penicillium dangeardii) is known to control Verticillium wilt of potato, artichoke, and olive. Talaromyces flavus isolate Tf-1 suppressed Verticillium wilt incidence in eggplant (Fravel and
Roberts, 1991). Purified glucose oxidase from T. flavus significantly reduced the growth rate of V. dahliae in the presence, but not in the absence, of
eggplant roots. This suggests that glucose from the roots is metabolized by
glucose oxidase to form hydrogen peroxide, which is toxic to V. dahliae
(Fravel and Roberts, 1991).
Coniothyrium
The fungal mycoparasite Coniothyrium minitans applied as a spray reduces Sclerotinia sclerotiorum (white mold) infection in bean, potato, carrot, and chicory (Gerlagh et al., 1999). C. minitans-based formulations have
been developed as Coniothyrin and Contans in Russia and Germany, respectively.
Nonpathogenic Isolates of Pathogens
Nonpathogenic isolates may induce resistance against pathogenic isolates of the same pathogen and other pathogens. Nonpathogenic isolates of
Rhizoctonia (np-R) protect seedlings against damping-off caused by virulent isolates of Rhizoctonia species of different anastomosis groups (Sneh,
1999). Some np-R isolates induced plant resistance against R. solani,
Pythium aphanidermatum, and Pseudomonas syringae pv. lachrymans in
cucumber (Sneh, 1999). Binucleate Rhizoctonia fungi are another group of
biocontrol agents that effectively control diseases of potato, bean, sugar
beet, cucumber, pepper, Catharanthus, and turf grass caused by Rhizoctonia
and Pythium spp. Colonization of host tissues by nonpathogenic isolates
triggers production of host defense compounds such as peroxidases, glucanases, and chitinases (Burns and Benson, 2000).
Reduced-pathogenicity isolates of Colletotrichum gloeosporioides (Glomerella cingulata) delayed anthracnose symptom development in avocado
fruits induced by virulent isolates (Yakoby et al., 2001). Preinoculation of
avocado fruit with reduced-pathogenicity isolates induced resistance that
was accompanied by an increase in the levels of preformed antifungal dienes
(Yakoby et al., 2001). Less aggressive strains of Ralstonia solanacearum induced resistance against aggressive strains of R. solanacearum (Trigalet
et al., 1998). Similarly, nonpathogenic isolates of Fusarium oxysporum induced resistance against pathogenic F. oxysporum strains in tomato, carnation, and sweet potato (Whipps, 1996). Commercial formulations of nonpathogenic strains of F. oxysporum and R. solanacearum are available in
Europe.
Ulocladium
The fungus Ulocladium atrum competes saprophytically with Botrytis
spp. during the colonization of necrotic plant tissues. The inoculum potential of B. cinerea is reduced by antagonistic interaction with U. atrum, leading to slower disease epidemic (Kohl et al., 1998). In cyclamen (Cyclamen
persicum), naturally senesced leaves within the dense canopy play a crucial
role in Botrytis epidemics. Since healthy leaves are normally resistant to
conidial infections, B. cinerea depends on dead tissues for initial entry into
the plant. Stimulated by this food base, the inoculum potential of the pathogen increases within the canopy of the single plant to such a level that
healthy petioles and leaf blades can then be infected (Kohl et al., 2000).
Biocontrol of B. cinerea by U. atrum could be achieved by competitively
excluding the pathogen from colonizing necrotic leaves present within the
cyclamen canopy. Repeated applications of conidial suspensions of U.
atrum controlled the disease as effectively as the growers standard fungicide program (Kohl et al., 1998, 2000).
Phialophora
Phialophora spp. are known to control wheat take-all caused by Gaeumannomyces graminis var. tritici. A Phialophora sp. (isolate I-52) was isolated from soil in a wheat field exhibiting suppression of take-all disease
(Mathre et al., 1998). I-52 was grown on a variety of autoclaved organic
substrates, including oat, millet, and canola seed. Each of these provided
significant disease control when added to the seed furrow. Seed treatment
was ineffective (Mathre et al., 1998). A Phialophora strain was commercialized in Australia (Wong et al., 1996).
Cryphonectria Hypoviruses
Some viruses may affect the virulence of crop pathogens. The hypovirulence caused by Cryphonectria hypoviruses (CHVs) on Cryphonectria
parasitica, the causal agent of chestnut blight, is a typical example for this
group (Robin et al., 2000). Infections by hypovirulent isolates result in superficial cankers on both European (Castanea sativa) and American (C.
dentata) chestnut trees, whereas virus-free isolates cause deep, lethal cankers. CHVs are cytoplasmic double-stranded RNA viruses that move into
conidia, but not into ascospores, and can be transmitted from an infected
isolate to a virus-free isolate through hyphal anastomosis (Robin et al.,
2000). In France, the Ministry of Agriculture has promoted an intensive re-
lease of hypovirulent isolates of C. parasitica for control of chestnut blight

on 180,000 ha since 1974 (Robin et al., 2000). Mixtures of hypovirulent isolates, selected according to the vegetative compatibility types present in the
populations of C. parasitica, were released in France. Every year in these
orchards, all newly formed cankers are treated with hypovirulent isolates by
introducing the mycelia into holes in the margin of the canker. This treatment shows a curative effect and contributes to the healing of the canker
when the virus is successfully transmitted. The hypoviruses have a preventative effect depending on the spread of hypovirulent strains within the area
where they are released. Hypoviruses are used to manage C. parasitica in Italy
also (Bisiach et al., 1991). The major problem in exploiting hypoviruses as
biocontrol agents is the existence of several vegetative compatibility (VC)
types in the populations of C. parasitica. Most of the virus isolates are specific to the VC types. The rate of transmission of the virus is negatively correlated with the number of vegetative incompatibility genes that differ between mycelia that anastomose. In North America, the lack of spread of
hypoviruses is characterized by high VC diversity. In Europe, low VC-type
diversity favors the spread of hypovirulent isolates of C. parasitica and the
recovery of European chestnut in many areas (Bissegger et al., 1997).
Bacillus Species
Different Bacillus spp. have been developed as microbial pesticides. Bacillus subtilis is available as a commercial formulation named FZB24. It is
produced in a multi-stage liquid fermentation process from a stock culture
that guarantees a uniform strain identity. The spores formed in this process
are separated from the culture broth and then dried and formulated together
with protective colloids, inert material, and other additives. The formulated
end product has a storage stability of at least two years. This product was
registered in Germany (Junge et al., 2000). The mode of action of this strain
has been reported. The bacterium might act by competition by temporary
colonization of the rhizosphere and rhizoplane. Bacillus subtilis has the
ability to form antibiotics in vitro, but the in vivo production of antibiotics
has not been demonstrated. Bacillus subtilis induces resistance by activation of defense genes. The bacterium also promotes plant and root growth,
probably by producing cytokinins and auxins. The stronger root system ultimately leads to an uptake of water and nutrients. The growth promotion
leads to the possibility of disease escape (Kilian et al., 2000). Bacillus
subtilis strain BACT-0 formulations with vermiculite or kaolin as carrier
material effectively control Pythium aphanidermatum infection in lettuce
(Amer and Utkhede, 2000). Bacillus cereus applied as seed treatment was as
effective as the fungicide metalaxyl in the management of seedling diseases

in lucerne (Kazmar et al., 2000). Bacillus subtilis strain EBW-1 as a root dip
effectively controls Agrobacterium tumefaciens crown gall in apples (Utkhede, 1999). Addition of nitrate to soil increased the efficacy of B. subtilis in
control of soil-borne fungal diseases (Knox et al., 2000). When used as a
spray, B. polymyxa decreases the spread of rose powdery mildew and rust on
leaves of Antirrhinum (Saniewska et al., 1998).
Pantoea
Pantoea agglomerans (Erwinia herbicola) is an effective biocontrol
agent against the apple and pear fire blight pathogen Erwinia amylovora
(Johnson et al., 2000). Pantoea agglomerans was sprayed on pear and apple
cultivars and the effect of environmental factors on the spread of the
biocontrol agent was assessed. The introduced bacteria colonized the blossoms of the inoculated trees, and temperature was found to be the important
variable affecting successful spread of this biocontrol agent from blossom
to blossom. The bacterial populations were positively correlated with mean
degree hours per day during bloom and negatively correlated with the proportion of days with rain (Johnson et al., 2000). Pantoea agglomerans is
spread by honey bees (Apis mellifera) in apple and pear orchards in New
Zealand (Vanneste et al., 1999) and the United States (Pusey, 1999). Pantoea agglomerans strain E25 was highly effective in the control of the fire
blight pathogen in the United States (Pusey, 1999). Pantoea agglomerans
effectively controls apple storage diseases caused by Botrytis cinerea and
Penicillium expansum (Sobiczewski and Bryk, 1999).
Lyophilized, talc-based, and whey-based formulations of P. agglomerans
were developed (Ozaktan et al., 1999). In these formulations, the bacteria
survived up to 180 days of storage at 10C and up to 60 days at 24C. Talcbased formulations were more effective in reducing pear fruit and blossom
blight caused by E. amylovora than the lyophilized and whey-based formulations (Ozaktan et al., 1999).
Agrobacterium, Serratia, Streptomyces, and Rhizobium
Agrobacterium radiobacter is an effective biocontrol agent that controls
crown gall of various crops caused by Agrobacterium tumefaciens. Commercial formulations of A. radiobacter are available in the United States,
Australia, and New Zealand (Whipps, 1996). Agrobacterium radiobacter
produces the bacteriocin agrocin 84, which inhibits A. tumefaciens. Agrobacterium radiobacter strains, which lack agrocin 84 production, are not ef-
fective against the crown gall pathogen. Increased doses of the antagonist
may reduce the disease severity only to certain extent. The amount of disease suppression per unit of antagonist dose decreased with increasing antagonist dose (Johnson and DiLeone, 1999).
Serratia marcescens controls several diseases. It controls Sclerotinia minor infection in lettuce (El-Tarabily et al., 2000). Serratia plymuthica strain
RIGG4 stimulates defense reactions in cucumber seedlings inoculated with
Pythium ultimum (Benhamou et al., 2000). The antagonist induced cell wall
apposition in cucumber, and callose, pectin, and cellulose appeared in the
wall appositions (Benhamou et al., 2000). Streptomyces griseoviridis is another important biocontrol agent. Commercial formulations of the bacterium are available and it controls Alternaria brassicicola in cauliflower and
damping off of pepper (Whipps, 1996). Some Rhizobium strains also acted
as biocontrol agents (Simpfendorfer et al., 1999).
COMMERCIALLY AVAILABLE MICROBIAL PESTICIDES
Commercialization of biocontrol agents as microbial pesticides requires
several steps, beginning with initial discovery and then proceeding through
testing of efficacy, prototyping, and then commercial production, extensive
large-scale field testing, toxicology and environmental tests, registration,
and marketing (Harman, 2000). Toxicological tests, such as oral, dermal,
ocular, respiratory, and health hazards using test animals and fish, should
show no adverse effects and the microorganism should not be a pathogen.
Success in selling biocontrol products requires that potential users and distributors be educated and convinced about the value of a biological product
that is probably more conceptually difficult to use than standard pesticides.
It requires several years and millions of dollars to bring a single biocontrol
agent to market and to become profitable. Hence, only a few biocontrol
agents have been developed as commercial products. They are mainly produced by small companies in different countries. Actizyme is a commercial
granulated formulation of Bacillus subtilis (Walker and Morey, 1999). TriD25 is a dry formulation containing Trichoderma koningii and T. harzianum
(Walker and Morey, 1999). FZB24 is a product of Bayer AG, Germany, and
it consists of B. subtilis (Kilian et al., 2000). The yeast Candida oleophila is
commercially available under the trade name Aspire (El-Ghaouth et al.,
2000). Two strains of the bacterium Pseudomonas syringae are available
under the trade names Biosave-100 and Biosave-110 (El-Ghaouth et al.,
2000). The following are the other commercially available microbial pesticides:
Agrobacterium radiobacterDiegall (Fruit growers Chemical Co., New

Zealand), Galltrol (AgBio Chem, Inc., California), Norbac 84-C (New
Bioproducts Inc., California), NoGall (Root Nodule, Pty Ltd., Australia; Bio-Care Technology, Pty Ltd., Australia)
Ampelomyces quisqualisAQ10 (Ecogen, Langhorne, Pennsylvania)
Bacillus subtilisKodiak, Kodiak (A-13), and Epic (MB 1600)
(Gustafson, Inc., Texas), Bactophyt (NPO Vector, Novosibirsk, Russia), System 3 (GBO3) (Helena Chemical Co., Tennessee)
Coniothyrium minitansConiothyrin (Russian Govt.), Contans (Prophyta
Biologischer Pflanzenschutz GmbH, Germany)
Fusarium oxysporum (nonpathogenic)Fusaclean (Fo47) (Natural Plant
Protection, Noguerres, France), Biofox-C (S.I.A.P.A., Bologna, Italy)
Gliocladium catenulatumPrimaStop (Kemira OY, Finland), PreStop
(Kemira OY, Finland)
Peniophora (Phlebia) giganteaPg suspension (Ecological Laboratory
Ltd., U.K.), Rotstop (Kemira OY, Finland)
Pseudomonas (Burkholderia) cepaciaIntercept (Soil Technologies,
Fairfield, Iowa), Blue Circle and Deny (CTT Corporation, Carlsbad,
California)
Pseudomonas fluorescensBioCoat (SandG Seeds, BV, the Netherlands),
Conqueror (Mauri Foods, Australia), Dagger (no longer available)
Pythium oligandrumPolygandron (Vyzkummy ustov rastlinnej, Slovak
Republic)
Ralstonia solanacearum (nonpathogenic)PSSOL (Natural Plant Protection, Noguerres, France)
Streptomyces griseoviridis Mycostop (Kemira OY, Finland), Stimagrow
(Kemira OY, Finland)
Trichoderma harzianum T-22Topshield, aka 1295-22, KRLAG2,
ATC 20847 (Bioworks, Geneva, NY), T-22G and T-22B (TGT Inc.,
New York), RootShield (Bioworks, Geneva, NY)
Trichoderma harzianumT-35 (Makhteshim-Agan Chemicals, Israel),
Harzian 20 and Harzian 10 (Natural Plant Protection, Noguerres,
France), F-stop (Eastman Kodak Co., United States TGT Inc., New
York), Supraavit (Bonegaard and Reitzel, Denmark)
Trichoderma harzianum strain T39Trichodex (Makhteshim-Agan
Chemicals, Israel)
Trichoderma harzianum + T. polysporumBINAB-T and W (Bio-Innovation AB, Toreboda, Sweden)
Trichoderma harzianum + T. virideTrichodowels, Trichoject, Trichopel,
and Trichoseal (Agrimm Technologies Ltd., New Zealand)
Trichoderma spp.Trichodermin (Bulgarian and Russian Governments),
Promot (JH Biotech, Inc., Ventura, California), Solsain, Hors-solsain,
Plantsain (Prestabiol, Montpellier, France), ANTI-FUNGUS

(Grondontsmettingen De Ceuster, Belgium), Ty (Mycontrol, Israel)
Trichoderma virens (Gliocladium virens)GlioGard and SoilGard
(Grace-Sierra Co., Maryland)
Trichoderma viridaeBip T (Poland)
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18
Biological
Biological
ControlMycorrhiza
ControlMycorrhiza
Mycorrhizal fungi are symbiotic organisms that live on the roots of several plants. Among them, endomycorrhizae are important because they confer resistance against pathogens. Several mycorrhizal fungi have been developed as commercial products and they can be used to reduce losses
caused by diseases.
WHAT IS A MYCORRHIZA?
Mycorrhiza is a symbiotic association of a fungus with the roots of a
plant. Three distinct classes of mycorrhiza are recognized: ectomycorrhiza,
endomycorrhiza, and ectendomycorrhiza. In the case of ectomycorrhiza,
the fungal symbionts penetrate intercellularly and partially replace the middle lamella between the cortical cells of the roots. This hyphal arrangement
around such cortical cells is called the Hartig net. The ectomycorrhizal
fungal symbionts also form a dense, usually continuous, hyphal network or
fungal mantle over the feeder root surface. Ectomycorrhizal association is
normally seen on tree species. The majority of ectomycorrhizal fungi belong
to the division Basidiomycota and the families of Amanitaceae, Tricholomataceae, Rhizopogonaceae, and Boletaceae. In contrast, the endomycorrhizal fungus penetrates the cortical cells of the feeder roots intracellularly.
Such fungal symbionts form large vesicles and arbuscules in cortical tissues, and hence, they are called vesicular-arbuscular mycorrhiza (VAM) or
arbuscular mycorrhiza (AM). Such fungi, however, do not form a dense
fungal mantle. Instead they develop a loose, intermittent arrangement of
mycelium with large spores on the root surface. Endomycorrhizal colonization takes place in many annual crops that do not form ectomycorrhiza, and
the fungal symbionts belong to the division Oomycota and the family
Endogonaceae.
Ectendomycorrhiza is the third class of mycorrhiza. This type of mycorrhiza is present on the roots of certain tree species under specific ecological
situations. This type of mycorrhiza resembles ectomycorrhiza, in that it
forms a Hartig net and a fungal mantle, and also resembles endomycorrhiza,
because of the intracellular penetration of cortical tissue by these fungi.

Mycorrhizal symbiosis plays a key role in nutrient cycling in the ecosystem
and protects plants against environmental stress (Barea and Jeffries, 1995).
Mycorrhizal fungi alter host physiology and induce biochemical changes in
the host metabolism. An altered host metabolism may result in resistance
against plant pathogens (Vidhyasekaran, 1990).
DISEASES CONTROLLED BY MYCORRHIZAL FUNGI
Since the 1970s, several researchers have demonstrated that endomycorrhizal fungi, such as Glomus mosseae, G. fasciculatum, G. intraradices,
and G. multiculae, could effectively control various diseases. The following
are important diseases that are controlled by mycorrhizal fungi (Caron,
1989; Jalali, 1990; Linderman, 1994; Azcon-Aguilar and Barea, 1996;
Cordier, 1998; Sharma and Adholeya, 2000):
Alfalfaroot rot (Thielaviopsis basicola), wilt (Verticillium
dahliae)
Citrusroot rot (Phytophthora parasitica), root rot (Thielaviopsis
basicola)
Cottonwilt (Verticillium dahliae), root rot (Thielaviopsis basicola)
Cucumberroot rot (Rhizoctonia solani), damping-off (Pythium spp.)
Onionpink root (Pyrenochaeta terrestris)
Pearoot rot (Thielaviopsis basicola)
Peanutroot rot (Sclerotium rolfsii)
Poinsettiadamping-off (Pythium ultimum)
Soybeanroot rot (Fusarium solani), root rot (Phytophthora
megasperma var. sojae)
Tobaccoroot rot (Thielaviopsis basicola)
Tomatowilt (Fusarium oxysporum f. sp. lycopersici), crown and
root rot (F. oxysporum f. sp. radicis lycopersici), root rot
(Thielaviopsis basicola), damping-off (Pythium
aphanidermatum), blight (Phytophthora parasitica)
Wheattake-all (Gaeumannomyces graminis)
MECHANISMS INVOLVED IN BIOCONTROL
BY MYCORRHIZAE
Several hypotheses have been proposed to explain the mechanisms
involved in the control of diseases by mycorrhizal fungi (Larsen, 2000).
Mycorrhizae may enhance nutrient uptake by plants and may strengthen the
plants against pathogens. These fungi enhance root growth, and robust root
development may compensate damage caused by pathogens. Exudates of
roots colonized by arbuscular mycorrhizal fungi may affect the pathogen
population in the mycorrhizosphere. Experiments in vitro showed that after
48 h in the presence of exudates from strawberry roots colonized by Glomus
etunicatum and G. monosporum, sporulation of Phytophthora fragariae
was reduced by about 64 and 67 percent, respectively (Norman and Hooker,
2000). A similar trend was observed in an in vivo system, with a 68 percent
reduction in sporulation of P. fragariae in the mycorrhizosphere of colonized plants relative to sporulation in the mycorrhizosphere of uncolonized
plants (Norman and Hooker, 2000).
An altered host metabolism may contribute to disease resistance induced
by mycorrhizae (Benhamou et al., 1994; Cordier, 1998). Accumulation of
phenolics in roots of plants due to mycorrhizal infection has been widely reported (Krishna and Bagyaraj, 1983; Cordier, 1998), and the role of phenolics
in disease resistance is known. Increased accumulation of phytoalexins due
to mycorrhizal infection has also been reported (Morandi et al., 1984; Harrison and Dixon, 1993). Chitinase transcripts accumulate in bean colonized
by arbuscular mycorrhizal fungus Glomus intraradices (Blee and Anderson, 1996). -1,3-Glucanase transcripts accumulate in and around arbuscule
containing cells (Lambias and Mehdy, 1995). Chitinases and -1,3-glucanases are important pathogenesis-related proteins that are involved in disease resistance (Vidhyasekaran, 1997, 2002). Dehne and Schoenbeck (1978)
showed increased lignification of cells in the endodermis of mycorrhizal tomato and cucumber plants. Lignification is involved in cell wall thickening.
Thus, mycorrhizal fungi may induce resistance against plant pathogens by
several methods.
MASS PRODUCTION OF MYCORRHIZAL FUNGI
Endomycorrhizal fungi are obligate symbionts and hence they cannot be
produced in a nutrient medium. They should be produced on living roots.
This method is tedious and the risk of contamination with pathogens exists.
AM fungi are mass multiplied on plants growing in disinfested soil. A
highly susceptible trap plant is used for the multiplication of AM fungi.
Stock cultures of these fungi are maintained in the form of colonized roots.
These roots (with spores) are used to produce large amounts of inoculum in
soil-based media. Soil in nursery beds are sterilized with methyl bromide.
The AM fungal propagules are added to the soil and seeds of the trap plant
(particularly monocots) are sown. The beds are watered regularly and kept
free from weeds. After about three months, the spore production in infected
roots is assessed and used for application in the field.
Soilless substrates are also used for multiplication of AM fungi. Shredded
bark, calcined montmorillonite clay, expanded clay aggregates, pertile, soilrite, and vermiculite have been used as inert substrates. The plants are
grown in these substrates in the presence of AM fungal propagules, with frequent addition of nutrient solutions. Colonized roots and spores can be produced in hydroponics. Precolonized plants on sterile substrate are needed
for this system. AM fungi can be propagated by growing precolonized
plants in a defined nutrient solution that flows over the host roots. In an
aeroponic system, the roots of the host are bathed in a fine mist of defined
nutrient solutions suspended in air. Root-organ culture (axenic culture) is
also used for multiplication of the mycorrhizal fungi (Sharma and Adholeya, 2000).
COMMERCIALIZATION OF MYCORRHIZAE
A few companies have taken up commercial production of arbuscular
mycorrhizas. AGC Microbio of Cambridge, United Kingdom, produces
mycorrhizal fungi in the trade name of Vaminoc. The other companies producing mycorrhizal fungi include: Bio-Enhancement Technologies, Camarillo, California; Horticultural Alliance, Sarasota, Florida; Plants Health
Care, Pittsburg, Pennsylvania; Tree Pro, West Lafyette, Illinois; Biological
Crop Protection, Wye, United Kingdom; Mikko-Tek Labs, Timmons, Ontario, Canada; Premier Tech, Quebec, Canada; Biorize, Dijon, France; Central Glass Co., Tokyo, Japan; and Global Horticare, Lelystad, The Netherlands.
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19
Breeding
Breeding
for Disease
forResistanceConventional
Disease Resistance
Breeding
Conventional Breeding
The most effective method of disease management is the use of diseaseresistant plant varieties. This can be highly cost-effective, and growers need
not spend anything to manage the diseases. Several genes are involved in
conferring disease resistance in plants. Several types of genetic resistance
have been reported. These resistances are described here. Epistasis, additive
effect, and interactive effect of clusters of resistance genes have been reported. Molecular markers linked to resistance genes were developed and
they are now used for selection of resistant plants from segregating populations of crosses. Various methods of breeding for disease resistance are described.
TYPES OF DISEASE RESISTANCE
Disease resistance can be classified into two major types: the host either
resists the establishment of a successful parasitic relationship by restricting
the infection site and the infection process, or it resists the subsequent colonization and reproduction of the parasite following successful infection.
The first type of resistance affects disease onset by reducing the amount
of effective initial inoculum. The other type of resistance affects the apparent infection rate and the amount of disease that finally develops in the
plants (Nelson, 1978). The first type of resistance can be called vertical resistance, qualitative resistance, race-specific resistance, specific resistance, major-gene resistance, monogenic resistance, complete resistance, or true resistance. The resistance is vertical because the
resistance is specific to races of the pathogen and is susceptible to other
races (a race is a taxon of pathogens, particularly characterized by specialization to different cultivars of one host species). The resistance is qualitative because the disease symptom is almost completely suppressed, mostly
by development of a hypersensitive pinpoint fleck reaction. It is called
race-specific/specific resistance because the resistance is specific against
races of the pathogen. It is called major-gene resistance because the resistance is governed by a major gene. Major genes are genes that have large,
distinct phenotypic expressions showing clear Mendelian segregation. The
resistance is also called monogenic resistance because it is governed by a
single gene. It is called complete/true resistance because the resistance is
expressed by complete suppression of disease symptom development.
The second type of resistance is called quantitative resistance, horizontal resistance, race-nonspecific resistance, nonspecific resistance,
minor gene resistance, polygenic resistance, general resistance, partial
resistance, or field resistance. It is called quantitative resistance because
the resistance is based on the amount of disease symptom development,
quantified by different infection types such as necrotic flecks, necrotic and
chlorotic areas with restricted sporulation, sporulation with chlorosis, abundant sporulation without chlorosis, lesion size, lesion area, etc. It is called
horizontal resistance because this resistance is against several races of the
pathogen. It is called race-nonspecific/nonspecific resistance because the
resistance is not specific to a particular race only. It is called minor gene resistance because the resistance is governed by minor genes. Minor genes
have small effects on the expression of the phenotype for resistance and
show quantitative segregation. This resistance is also called polygenic resistance because it is governed by several genes. It is called general/partial/
field resistance because this resistance is only partial and field tolerant.
Although these definitions are attractive, there are many exceptions to
this broad classification. Preinfection factors may play a role in some quantitative/horizontal resistance, and sporulation is not affected in some qualitative/vertical resistance (Nelson, 1978). Separate major and minor genes
may not exist. Whether major or minor, either kind of gene is inherited the
same way and is capable of showing similar effects. The degree of expression is modified by environmental contribution to a large extent. Further,
what works as a major gene in one genetic background could work as a minor gene in another background. Expression of resistance (major or minor)
genes may be influenced or modified by other resistance genes in a different
genetic background. When many major genes express together, they function exactly like minor genes.
Genetic resistance may vary depending on host age and environmental
factors. Seedling resistance works when plants are young, and adult plant
resistance works when the plants are mature. The wheat stem rust resistance
gene Sr25 from Thinopyrum elongatum is highly effective in the seedling
stage of the wheat plant, but loses its effectiveness to some extent in adult
wheat plants that are near maturity. The stem rust resistance gene Sr2 is best
expressed in adult wheat plants (McIntosh et al., 1995). The changes in host
physiology during maturity would have contributed to this type of resis-
tance. Seedling resistance may be based on major genes, whereas adult

plant resistance is generally polygenic. However, exceptions are also common. Adult plant resistance of the durum wheat cultivar Glossy Huguenot is
controlled by a single dominant gene (McIntosh, 1992). Temperatures may
alter the expression of some resistance genes. The wheat stem rust resistance genes Sr6, Sr12, Sr15, Sr17, Sr22, Sr34, and Sr38 are generally more
effective at temperatures below 20C. Genes Sr13 and Sr23 normally show
more effectiveness at higher temperatures (McIntosh et al., 1995).
Two types of resistance genes have been recognized: recessive genes and
dominant genes. A recessive gene is a gene that is phenotypically manifest
in the homozygous state, but is masked in the presence of its dominant allele
or dominant gene. Usually the dominant gene produces a functional product, whereas its recessive does not. The normal phenotype is produced if the
dominant allele is present, and the mutant phenotype appears only in the absence of the dominant allele, i.e., when the recessive gene is homozygous.
For example, wheat stem rust resistance genes Sr2 and Sr17 are recessively
inherited, whereas all other Sr genes show dominant inheritance (McIntosh
et al., 1995).
INTERACTION BETWEEN RESISTANCE GENES
Plants are endowed with several disease resistance genes. The interaction
between these genes may vary. Epistasis, additive effects and interactive effects of clusters of resistance genes have been reported. Epistasis is generally observed when two or more wheat stem rust resistance Sr genes are
present together. Only the gene conditioning the lowest infection type is expressed. An additive effect of resistance genes is observed in many plants.
The wheat stem rust resistance genes Sr7a and Sr12 were observed to interact, resulting in significantly higher levels of resistance than that conferred
by either gene acting alone (McIntosh et al., 1995). An interactive effect of
certain genes has also been reported. A gene conferring resistance to one
disease may interact with a gene conferring resistance against another unrelated disease and enhance or suppress the action of the latter gene. Some
suppressors that inhibit the expression of relevant resistance genes may
be present on chromosomes. Suppressors on chromosomes 1D and 3D were
reported to prevent the expression of stem rust resistance genes present on
chromosomes 1B, 2B, and 7B in wheat. The wheat cultivar Thatcher and the
backcross derivative Canthatch were susceptible to several stem rust races
because of a suppressor on chromosome 7DL that inhibited the expression
of relevant resistance genes. Thatcher lines possessing the leaf rust resis-
tance gene Lr34 were more resistant to stem rust than Thatcher. In Thatcher
and backcross derivatives, Lr34 inactivates the 7DL suppressor (Kerber and
Aung, 1999). Fast rusting to stem rust was reported in lines carrying leaf
rust resistance genes Lr28 and Lr32, suggesting that these genes might have
suppressed the action of stem rust resistance genes.
MOLECULAR MARKER-ASSISTED SELECTION
The utility of molecular markers in resistance breeding is based on finding tight linkages between these markers and disease resistance genes. Such
linkage permits one to infer the presence of the disease resistance gene by
assaying for the marker. Incorporation of disease resistance genes into the
susceptible varieties requires crosses with stocks that carry the resistance
genes, followed by selection among the progeny for individuals possessing
the disease resistance gene combination. Traditionally, progeny are screened
for the presence of disease resistance genes by inoculation with the pathogen. However, simultaneous or even sequential screening of plants with several different pathogens can be difficult or impractical. Screening for resistance to new pathogens is also difficult because of quarantine restrictions on
their shipment and use. In contrast, detecting disease resistance genes by
their linkage to genetic markers makes it practical to screen for many different disease resistance genes simultaneously without the need to inoculate
the population (Tanksley et al., 1989). Only the advanced generation would
be required to be tested in disease nurseries. Based on host-pathogen interaction alone, it is often not possible to discriminate the presence of additional resistance (R)-genes (masking effect). With molecular marker-assisted selection (MAS), new R-gene segregation can be followed even in the
presence of existing R-gene. Hence, R-genes from diverse sources can be
incorporated into a single genotype for durable resistance. Pyramiding of
resistance genes to fight against several races of the pathogen can be done
using molecular markers.
Several types of molecular markers have been used to select resistance
genes. These include restriction fragment length polymorphism, randomly
amplified polymorphic DNA, simple sequence repeats (SSRs) or microsatellites, and polymerase chain reaction (PCR)-based DNA markers, such
as sequence characterized amplified regions (SCAR), sequence-tagged sites
(STS), and amplified fragment length polymorphisms (AFLP). RFLP is a
technique in which plants may be differentiated by analysis of patterns derived from cleavage of their DNA. Genomic DNA from susceptible and resistant plants is isolated and cut with restriction endonuclease and size frac-
tionated by gel electrophoresis. The DNA is transferred from the gel to a

nitrocellulose filter and probed with a small piece of DNA (marker) in a
Southern blot type experiment. The DNA fragment, when used to probe
Southern blots of restricted genomic DNA from different plants, allows the
visualization of variations in the size and/or number of the restriction fragments generated from the susceptible and resistant plants. RAPD and PCRbased techniques were described in Chapter 13. PCR can specifically amplify hypervariable regions within defined loci. These minisatellite DNA
regions consist of tandemly repeated motifs of 15 to 30 nucleotides at various loci. Similarly, PCR can amplify hypervariable arrays of tandemly arranged simple sequence motifs of only two to ten nucleotides to detect
DNA polymorphism. Another PCR-based technique is called amplification fragment length polymorphism (AFLP). It uses single oligonucleotide
primers to amplify discrete but arbitrary stretches of DNA from a target genome. Amplification is done using a thermostable DNA polymerase.
Marker-assisted selection was used to select quantitative resistant lines
against northern corn blight (Exserohilum turcicum) by using 87 RFLP
markers and seven SSR markers (Welz and Geiger, 2000). RFLP and
microsatellites or simple sequence repeats (SSRs) were used as genetic
markers to select Rsv, a gene conferring resistance to Soybean mosaic virus
in soybean (Yu et al., 1994). The presence of xa13, a recessive bacterial
blight resistance gene in rice could not be identified by conventional approaches because the effect of xa13 was masked by the dominant resistance
gene Xa21, which was also present in those plants. The presence of xa13
could be identified by the RFLP marker RG136 (Zhang et al., 1996). MAS
is especially useful in selecting for recessive genes, such as xa13, where the
presence of the gene in the heterozygous condition cannot be detected through
traditional approaches without progeny testing. Six molecular markers have
been identified to be linked to the rice bacterial blight resistance gene Xa21.
They can be identified by RFLP and STS. These six molecular markers can
be utilized in marker-assisted breeding in rice (Williams et al., 1996).
Two randomly amplified polymorphic DNA markers, OPF62700 and
OPH182400, that are tightly linked to Pi-10, a dominant blast-resistance
gene conferring complete resistance to race IB46 of the blast fungus,
Pyricularia grisea, could be identified (Naqvi and Chattoo, 1996). Sequence characterized amplified regions (SCARs) markers were derived
from OPF62700 and OPH182400, and these amplified RAPD products were
cloned and sequenced. Nucleotide sequence information, obtained for each
end of the two linked RAPD markers, was used to design 24-mer oligonucleotide primers for PCR amplification of the respective SCARs. Polymorphisms appearing as differences in the length of the SCARs alternate
alleles were considered for the indirect selection of Pi-10. Such poly-
morphisms converted the linked dominant RAPD loci into codominant

SCAR markers. The SCAR markers will be useful in marker-assisted selection of the Pi-10 locus (Naqvi and Chattoo, 1996).
Two SCAR markers were developed for the strawberry gene Rpf1, which
confers resistance to red stele root rot caused by Phytophthora fragariae
var. fragariae. The scar markers were developed originally from the sequence of RAPD OPO-16C(438) (Haymes et al., 2000). At least five RFLP
marker alleles were present in all scab (Venturia inaequalis)-resistant selections arising from Malus floribunda (King et al., 1999). This was confirmed
by SCAR assays and Southern-hybridization experiments. None of the
marker alleles were present in the susceptible accessions (King et al., 1999).
SCARs were developed based on nucleotide differences within resistance
gene-like fragments isolated from a potato carrying the Ryadg gene, which
confers extreme resistance to Potato virus Y. Two SCAR markers showed
high accuracy for detection of the Ryadg gene (Kasai et al., 2000).
Fifteen AFLP markers were found linked to Fom-2 gene, which confers
resistance to races 0 and 1 of Fusarium oxysporum f. sp. melonis in melon
(Wang et al., 2000). RAPD markers to the gene were also developed.
Cleaved amplified polymorphic sequences (CAPS) and RFLP markers
were developed by converting RAPD markers E07 and G17, respectively
(Zheng et al., 1999). Both CAPS and RFLP markers were easier to score
and more accurate and consistent in selecting the Fusarium wilt-resistant
phenotype than the RAPD markers from which they were derived (Zheng
et al., 1999). Fifteen AFLP markers were tightly linked to the Vf gene conferring scab (Venturia inaequalis) resistance in apple. Eleven of these
AFLPs were converted into SCAR markers. Three of the converted SCAR
markers were inseparable from the Vf gene, and these markers are useful in
marker-assisted selection (Xu et al., 2001).
PCR-based markers were developed for use in marker-assisted selection.
A DNA fragment (ADG2) that is 77 percent homlogous to the gene N (resistant to Tobacco mosaic virus) and 53 percent homologous to RPP5 (resistant
to Peronospora parasitica in Arabidopsis thaliana) was amplified by PCR
from the diploid potato genotype that carries the gene Ryadg located on chromosome X1 and conferring extreme resistance to Potato virus Y (Sorri et al.,
1999). This PCR-based resistance marker, developed using a resistance analogue, will be useful in selecting resistant plants. To facilitate the transfer of
the leaf rust resistance gene Lr47 to commercial wheat varieties, the completely linked RFLP locus Xabc465 was converted into a PCR-based marker
(Helquera et al., 2000).
NEAR-ISOGENIC LINES (NIL)

Near-isogenic lines are obtained by crossing a donor plant carrying the
resistance gene with a susceptible recipient parent, followed by repeated
backcrosses to the recipient parent under continuous selection for the resistance trait. The donor genome is therefore progressively diluted in the introgressed lines until a short chromosomal segment is obtained around the
resistance gene. When the parents and the NIL derived from them are compared with the molecular probes in NILs, only those that are linked to the resistance locus are expected to be polymorphic. NILs can be useful to select
the probes for resistance genes.
COMMON BREEDING METHODS
FOR DISEASE RESISTANCE
The most common method of breeding for disease resistance is pedigree
selection. In this method, crosses are made between two parents (one with
the resistance trait and another with good agronomic traits) and the individual plants are selected for resistance from the F2 generation. These selections are allowed to produce seed for the next generation. The selection procedure is repeated in each generation, and a higher proportion of resistant
plants is obtained in each successive generation until homozygosity is obtained.
In the bulk population selection method, early segregating generations,
usually F2 to F5, are bulked together without selection. In later generations,
when most plants are homozygous, individual plants are selected for resistance and their progenies are evaluated for resistance as in the pedigree
method.
In backcross breeding, the hybrid derived from a cross between the donor
plant (with the resistance trait) and a recurrent parent (susceptible, but with
good agronomic characters) is crossed back to the recurrent parent and the
progeny are screened for the disease resistance trait by inoculation with the
pathogen or by using molecular markers tagged to the resistance genes. The
selected individuals are crossed again to the recurrent parent and the process
is repeated. The selection procedure is repeated in each generation, and
hence it is called recurrent selection. After several cycles, plants are obtained that are nearly identical to the recurrent parent, with the exception
that genes for resistance have been added (Tanksley et al., 1989).
The introduction of molecular markers helps to overcome major limitations of backcross breeding. If the gene(s) to be transferred are marked by
tightly linked molecular markers, segregating populations of plants can be
screened at the seedling stagebefore the trait is expressedfor the presence of the resistance genes. Because the molecular markers can be used to
mark quantitative trait loci (QTLs) as well as major genes, there are no limitations to the types of resistance that can be manipulated by the backcross
selection.
Breeding for Disease Resistance Using Major Genes
Breeding strategies for major gene disease resistance involve identification of resistance genes and incorporating them into high-yielding, but susceptible varieties through pedigree/backcross breeding. Identification of
disease resistance genes is carried out with the help of conventional genetic
analysis employing simple inheritance studies and tests of allelism. The
matching avirulence/virulence test and the gene linkages are also useful
means to identify resistance genes. Several disease-resistant varieties have
been developed to exploit major gene resistance. More than 40 loci for resistance to wheat stem rust have been identified and designated as Sr genes.
Most of the designated Sr genes were derived from Triticum aestivum. However, a number of genes were derived from other Triticum spp., Secale
cereale, and Thinopyrum elongatum. Several cultivars were developed using these genes.
Gene-for-gene hypothesis works among the major gene resistances. According to Flor (1946, 1961), a gene for resistance in the host corresponds to
a gene for avirulence in the pathogen. No resistance occurs unless a resistance allele is present in the host along with a corresponding avirulence
gene in the pathogen (Johnson and Knott, 1992). Several races exist in each
pathogen, particularly in the case of biotrophic pathogens, and these races
contain different avirulence genes. Hence, major gene resistance, which is
specific against particular races, breaks down quickly whenever new races
occur (Welz and Geiger, 2000). For example, 239 races of the wheat leaf
rust pathogen, Puccinia triticina, were detected from eight wheat-growing
regions of the United States from 1984 to 1999 (Long and Leonard, 2000).
Puccinia recondita f. sp. tritici race 104-1,2,3,6,7,9,11, with virulence. Virulence for the wheat leaf rust resistance gene Lr26 became widespread in
Australia in 1997. This resulted in the withdrawal from cultivation of the
wheat cultivar Mawson, which possesses Lr26. Barley varieties resistant to
the powdery mildew pathogen Blumeria graminis f. sp. hordei have been
developed by incorporating various resistance genes, including Va6, Va7,
Va8, Va9, Va12, Vh, Va3, Va13, and V(Me). Virulences to all these genes
were detected in the population of the pathogen in Lativia (Rashal et al.,
2000). Breeding for resistance that exploits major genes often results in the
evolution of a matching virulence within the pathogen population, leading

to an apparent breakdown of resistance. This leads to boom-bust cycles,
in which varieties possessing effective resistance are grown on an expanding acreage (boom) until a matching virulence evolves and spreads within
the pathogen population (bust) (Pink and Puddephat, 1999).
Hence, strong genes, which show resistance to many races, should be used
for breeding for major gene resistance. The wheat stem rust resistance gene
Sr26, which is derived from Thinopyrum elongatum, shows resistance to all
stem rust cultures obtained worldwide (McIntosh et al., 1995). The wheat
leaf rust resistance gene Lr19 shows resistance to the leaf rust cultures collected worldwide, except those from Mexico.
BREEDING FOR QUANTITATIVE (PARTIAL) RESISTANCE
USING QTL LINKED WITH MARKERS
Compared to major genes, partial resistance is difficult to manipulate in a
breeding program. It is difficult to identify quantitative resistance. For example, quantitative resistance in spring barley cultivars with different genetic bases of resistance to scald is assessed by measuring the variables,
such as the disease progress curve (AUDPC), apparent infection rate (AR),
infection frequency (IF), lesion length (LL), lesion width (LW), time to disease onset at 5 percent severity (T5), final disease severity (FS), and
sporulation (SP) (Xi et al., 2000). Not all of the variables may equally contribute to disease resistance. For example, sporangia production was significantly correlated to the area under disease progress curve, whereas the days
to 5 percent severity threshold was negatively correlated with AUDPC in
potato crosses inoculated with the late blight pathogen, Phytophthora infestans (Dorrance et al., 2001). It may be difficult to measure all these variables
in segregating populations to select resistant plants. These variables are also
affected by environmental factors.
It may be more difficult to manipulate quantitative traits in breeding programs than single gene traits. If complex traits could be resolved into their
individual components, it might then be possible to deal with these traits
similar to single gene traits. Molecular markers that are closely linked to
genes coding for partial resistance could enable breeders to manipulate the
trait by selecting for the presence of the markers associated with each of a
set of minor genes.
Most of the complex quantitative traits are under the control of many
genes. The genetic loci associated with complex traits are called quantitative trait loci (QTLs). Linkages between single genetic markers and QTLs
have been reported in many cases. However, associating specific QTLs with
genetic markers and locating them on a linkage group is a great task for conventional genetic analysis, because such genetic markers are not available.
This limitation is remedied by the construction of complex molecular
marker maps that permit systematic searches of an entire genome for QTLs
influencing a trait. Molecular markers can be used to select QTLs influencing quantitative disease resistance. A cross is made between susceptible and
quantitative resistant plants. The segregating progeny are obtained from the
hybrid (commonly F2, backcross, or recombinant inbred lines). A number
of progeny are evaluated for the QTLs and for their genotypes at the molecular marker loci at regular intervals (10 to 20 cM) throughout the genome. A
search is then made for associations between the segregating molecular
markers and the QTLs. If such associations are found, they should be due to
linkage of the molecular marker to a gene(s) affecting the QTL (Tanksley et
al., 1989). In this way, several QTLs can be identified and used for selecting
quantitative resistant plants. To select for quantitative resistance, backcross
breeding with a recurrent selection method using molecular markers is preferred (Bai and Shaner, 1994; Veillet et al., 1996).
Several QTLs have been detected and used in resistance breeding. The
inheritance of powdery mildew and black rot resistance in grape (Vitis
vinifera) was studied by using the cross Horizon (complex interspecific
hybrid) + Illinois 547-1 (Vitis rupestris + V. cinerea) (Dalbo et al., 2000).
A major QTL was found in Illinois 547-1, the resistant parent. A single
RAPD marker, CS25b, associated with this QTL accounted for approximately 41 percent of the phenotypic variation. Specific markers, such as
Cleaved amplified polymorphic sequences (CAPS) developed for the CS25b
locus, were also useful to separate resistant and susceptible individuals in
the progenies of the crosses (Dalbo et al., 2000).
Breeding for Qualitative (Complete) Resistance with Additive Effects
of Quantitative (Partial) Resistance Genes
A few genes in combination contribute to slow-rusting resistance in
wheat to leaf rust (Puccinia triticina) and stripe rust (Puccinia striiformis).
Inheritance studies showed that individually these genes have small to intermediate effects only; combinations of three to five such genes result in a
high level of resistance. Near-immunity to leaf and stripe rusts in wheat
could be obtained by combining slow-rusting resistance (partial resistance)
genes (Singh et al., 2000). Simple crosses were made between parents that
had moderate to good levels of resistance to both rusts. The F1s were then
top-crossed (three-way) with a third parent that had high yield potential and
at least some resistance. From segregating generations, lines that contain
high yield potential and resistance levels reaching near-immunity to both

rusts could be selected. This resistance was based on additive genes (Singh
et al., 2000).
Pyramiding of Genes
Combining different genes in a single plant is called pyramiding of
genes. A pyramid can be constructed with major genes and minor genes.
Pyramiding different resistance genes into a genotype will facilitate in developing durable resistant varieties. Liu et al. (2000) developed powdery
mildew resistant wheat varieties by molecular marker-facilitated pyramiding of different genes. Genes (Pm) for resistance to powdery mildew
(Erysiphe graminis f. sp. tritici) were identified in wheat at more than 30
loci. Molecular markers tightly linked to about 10 of these Pm genes were
identified. Three of these genes, Pm2, Pm4a, and Pm21, were used for
marker-facilitated pyramiding. The following is the procedure followed by
Liu et al. (2000) for pyramiding: First, near-isogenic lines (NILs), Yang 93111 (Pm4a), Yang 94-143 (Pm2), and Yang 96 (487) (Pm21) were developed by six to nine backcrosses with the local susceptible cultivar Yang
158 as the recurrent parent. These lines were proved to include Pm4a, Pm2,
and Pm21, respectively, by artificial inoculation and with suitable RFLP
markers. The NILs had morphological and agronomic attributes that are
similar to their recurrent parent Yang. To pyramid the resistance, crosses
were made between the NILs, i.e., Yang 93-111(Pm4a) Yang 94-143
(Pm2), Yang 94-143 (Pm2) Yang 96 (487) (Pm21), Yang 93-111
(Pm4a) Yang 96 (487) (Pm21). The resulting F2 populations were
planted and screened at the seedling stage. A total of 75 F2 plants from cross
Yang 93-111 Yang 94-143 were obtained. RFLP with the probes
BCD1871 and Whs 350 identified 53 plants carrying Pm2. Furthermore,
RFLP analysis with probe BCD 1231, showed that 39 of these 53 plants also
carried Pm4a (Liu et al., 2000).
Even defeated major resistance genes can be used for pyramiding for developing resistant varieties. Xanthomonas vesicatoria races 1, 3, 4, and 6
caused similar high levels of disease severity, followed by races 2 and 5 on
susceptible bell pepper variety Early Calwonder (ECW). Race 3 caused severe disease on all isolines lacking resistance gene Bs2. Race 4, which defeats Bs1 and Bs2, caused less disease on isoline ECW-12R (which carries
Bs1 + Bs2), than on isolines ECW, ECW-10R (which carries Bs1), and
ECW-20 R (which carries Bs2). Race 6, which defeats all three major genes,
causes less disease on isoline ECW-13R (Bs1 + Bs3) and ECW-123R (Bs1+
Bs2+ Bs3) than on isolines ECW, ECW-10R, ECW-20R, and ECW-30R
(Bs3). Defeated major resistance genes deployed in specific gene combinations were associated with less area under the disease progress curve than
when genes were deployed individually in isolines of ECW (Kousik and
Ritchie, 1999). These results suggest that by pyramiding even defeated
genes, improved resistance can be obtained.
Mutation Breeding
Some disease-resistant varieties have been developed by mutation breeding using physical or chemical mutagens. The wheat variety Sinvalocho
gama against Puccinia graminis f. sp. tritici, the oats variety Alamo-x
against Puccinia coronata, the rice varieties Fulgenate and RD 6 against
Pyricularia oryzae, and the bean varieties Universal and Unima against
Colletotrichum lindemuthianum were developed by mutation breeding (Vidhyasekaran, 1993a). Two mutants, I3-48 and I3-54, obtained by irradiation
with fast neutrons from wheat cv. Hobbit sib were found to be significantly
more resistant than Hobbit sib to Erysiphe graminis f. sp. tritici. Constitutive expression of a thaumatin-like protein gene was detected in both I3-48
and I3-54, but not in Hobbit sib (Duggal et al., 2000).
Durable Resistance
Durable resistance is resistance that remains effective in a cultivar that is
widely grown for a long period of time in an environment favorable to the
disease. Durable resistance cannot be identified immediately after or at the
time of release of a variety, as it should be grown for a long time. Durable resistance cannot be identified under greenhouse conditions or in experimental station farms because the crop should be grown in large areas. Durable
resistance can probably be identified based on relative performance of other
cultivars during this period. Durable resistance may be a race-nonspecific or
horizontal resistance, partial resistance, field resistance, or quantitative resistance. Even a single major gene can contribute to durable disease resistance. A number of Australian wheat varieties carrying the stem rust resistance
gene Sr26 derived from Thinopyrum elongatum have remained resistant to
stem rust since 1967, despite being grown over a large area (McIntosh et al.,
1995). Sometimes, even partial resistance may break down quickly. A
wheat cultivar Joss Cambier possessing partial resistance to stripe rust
(Puccinia striiformis) released in the United Kingdom in 1969 became
highly susceptible in 1971 due to a new race of P. striiformis (Johnson,
1993).
Several factors may contribute to durable resistance. The race-specific

(major gene) resistance genes to the potato viruses A, X, and Y have shown
no signs of losing effectiveness for more than 60 years in Europe. The more
inoculum of a pathogen is present, the greater the chances that new races
can arise. In Western Europe, potato viruses are kept at a reasonably low
level due to a thoroughly controlled system of seed potato production. Due
to a decreased amount of inoculum, new races may not have developed during these years (Parlevliet, 1993). When the crop is a minor crop (grown in
limited, discontinuous areas), resistance may last longer. In flax in North
America, the race-specific major genes against flax rust (Melampsora lini)
lasted from 10 to more than 13 years and the flax is grown only in small areas (Parlevliet, 1993).
The parasitic ways of life of pathogens may also determine the durability
of major gene resistance in the field. Race-specific resistance against biotrophs (pathogens that exploit living cells, such as rusts and powdery mildews) and hemibiotrophs (pathogens that are biotrophic at the lesion perimeter, but necrotrophic near the lesion center, such as Phytophthora infestans
in potato and Rhynchosporium secalis in barley) breaks down quickly due to
the development of new races. Resistance against necrotrophs (pathogens
that exploit host tissue after killing it with toxins, such as Septoria leaf and
glume blotch in wheat and Periconia circinata in sorghum), is generally durable.
The degree of host specialization of pathogens may also determine durability of resistance (Parlevliet, 1993). Formae speciales of Puccinia graminis and Erysiphe graminis are highly host-specific in cereals. Viruses are
often less specialized and they have a wide host range. Several necrotrophs
can infect multiple hosts. The means of dispersal of pathogens, such as soilborne, seed-borne, airborne, and splash-borne, is also important in the development of new races. Pathogens in which new races very often occur are
biotrophic or hemibiotrophic, airborne, or splash-borne fungi. Major gene resistance against these pathogens breaks down very often.
Another group of pathogens exists, in which only a few races are known
and the major gene resistance persists for longer time. Viruses such as Tobacco mosaic virus, Bean common mosaic virus, and Pea seed-borne mosaic virus, and fungi such as Fusarium oxysporum and Peronospora tabacina belong to this group. In the third group of pathogens, no races are
known and the resistance used has been effective from the start (Parlevliet,
1993). Fungi such as Cladosporium cucumerinum in cucumber, Pseudocercosporella herpotrichoides in wheat, and Helminthosporium victoriae in
oat belong to this group.
Several quantitative-resistance varieties show durable resistance. Breeding
strategies, including pyramiding of major resistance genes and introgres-
sion of additive minor genes in an adapted background, may result in rapid

progress in the breeding of durable disease-resistant varieties.
Multiline Mixtures
To avoid rapid breakdown of disease resistance in the field, multiline
cultivars were developed (Wolfe, 1985). Two types of multilines are used.
Clean crop multilines are those in which each variety accords a broad
spectrum of resistance. Dirty crop multilines are those in which a susceptible variety is purposefully introduced in the multiline mixture. The susceptible variety will reduce the selection pressure on the pathogen and retard the appearance of new races. Host mixtures may restrict the spread of a
disease considerably relative to the mean disease level of the components.
Reduction up to 80 percent in powdery mildew infection compared with the
mean disease level of the component grown as pure stands was reported in
barley (Wolfe, 1985). Reduction in disease incidence may be due to the decrease in the spatial density of susceptible plants. This decrease limits the
amount of susceptible tissue in a given area. It also reduces the probability
of survival of spores that leave each parent lesion. Spore density declines
exponentially along a gradient from the source (Vidhyasekaran, 1993b). Varietal mixture may reduce the disease incidence due to the barrier effect provided by resistant plants that fill the space between susceptible lines. Reduced disease incidence may also be due to the resistance induced by
incompatible pathogenic spores. Induced resistance is always greater than
induced susceptibility. Induced resistance in varietal mixtures will be great.
In the varietal mixture, slower adaptation of the pathogen is common compared to single variety cultivation.
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20
Breeding
Breeding
for Disease
for ResistanceGenetic
Disease Resistance
Engineering
Genetic Engineering
Genetic engineering technology has been exploited to develop diseaseresistant varieties. Plant disease resistance genes and defense genes have
been cloned. Transgenic plants expressing these genes have been developed, and they show resistance against fungal, bacterial, and viral diseases.
Genes from fungi, bacteria, viruses, insects, and human beings have been
transferred to plants, and transgenic plants expressing these genes show resistance against various diseases. The use of genetic engineering technology in the development of disease-resistant plants is discussed in this chapter.
TRANSGENIC PLANTS EXPRESSING
PLANT RESISTANCE GENES
Several fungal disease resistance genes have been cloned. Cf9, Cf2, Cf4,
and Cf5 genes in tomato against Cladosporium fulvum, I2 in tomato against
Fusarium oxysporum f. sp. lycopersici, Pi-b and Pi-ta in rice against rice
blast pathogen, LR10 against leaf rust in wheat, HM1 against Helminthosporium maydis, L6 and M genes in flax against rust, and RPP5 against
Peronospora parasitica in Arabidopsis thaliana have all been cloned. Disease resistance genes found in one plant species have also been detected in
other plants. Analogs of cloned resistance genes have been detected in rice,
wheat, barley, cotton, soybean, pepper, chickpea, flax, tomato, tobacco, and
Brassica napus. Hundreds of homologues of disease resistance genes have
been identified from various crops.
Resistance genes can be cloned from resistant varieties and transferred to
susceptible but high-yielding varieties, making these plants resistant as
well. The resistance genes can even be transferred to heterologous plants.
The gene Cf9 from tomato, which confers resistance against Cladosporium
fulvum, has been transferred to oilseed rape. The transgenic oilseed rape
shows resistance against Leptosphaeria maculans (Hennin et al., 2000).
Several bacterial disease resistance genes have also been cloned. These
include Pto, Pti, and Prf genes in tomato against Pseudomonas syringae pv.
tomato, Xa21 and Xa1 genes in rice against Xanthomonas oryzae pv. oryzae,
and RPS2 and RPM1 in Arabidopsis thaliana against Pseudomonas syringae.
Functional homologues of the resistance genes RPM1 and RPS2 detected in
Arabidopsis thaliana could be detected in pea (Pisum sativum), bean (Phaseolus vulgaris), and soybean (Glycine max). Two disease resistance genes
cloned from soybean are similar to RPS2 in Arabidopsis.
The bacterial disease resistance genes cloned from resistant varieties
could be transferred to susceptible but high-yielding varieties, making these
plants resistant as well. The resistance gene Xa21 has been cloned from a
wild rice, Oryza longistaminata. The cloned gene could be transferred to
different rice cultivars varying widely in their phenotypic characters, such
as O. sativa ssp. japonica and O. sativa ssp. indica types (Wang et al., 1996;
Tu et al., 1998). The japonica rice cultivar TP309 was transformed with the
Xa21 gene by particle-bombardment technique. The transgenic plants expressing the Xa21 gene showed resistance to several races of the pathogen
(Wang et al., 1996). The indica rice cultivar IR72 was transformed with the
Xa21 gene, and the transgenic plants showed resistance to both of the tested
races (race 4 and race 6) of X. oryzae pv. oryzae (Tu et al., 1998). The transgenic Xa21 plants showed greater resistance to various isolates of the pathogen as compared to that of the donor cultivar IRBB21. In addition, about a
five to ten times stronger Xa21-specific hybridization was observed in the
transgenic Xa21 plants as compared to that of the donor line, indicating
multiple insertions in a single locus in the transformed lines (Wang et al.,
1996). Multiple copies of transgenes are often inherited as a single locus in
transgenic lines generated by particle bombardment. Transgenic tomato
plants expressing the cloned Pto gene showed high resistance to P. syringae
pv. tomato (Martin et al., 1993).
A bacterial disease resistance gene from one plant species could be successfully transferred to another plant species. The cloned Pti gene from tomato was transferred to tobacco, and the transgenic plants showed resistance to P. syringae pv. tabaci (Zhou et al., 1995). The transgenic tobacco
plants expressing the cloned tomato Pto gene showed resistance to P.
syringae pv. syringae strains. Similar resistance was observed in Nicotiana
benthamiana against P. syringae strains (Rommens et al., 1995; Thilmony
et al., 1995).
So far, only three virus-disease resistance genes have been cloned. These
include the N gene in tobacco against the Tobacco mosaic virus (TMV)
(Whitham et al., 1996), Sw-5 in tomato against tospoviruses (Brommonschenkel et al., 2000), and the Rx gene in potato against Potato virus Y
(PVY) (Bendahmane et al., 1999). However, several homologues of these

disease resistance genes have been detected in other plants.
The resistance genes cloned from resistant varieties have been transferred to the susceptible varieties, and these transgenic plants expressed resistance. Transgenic potato plants expressing the Rx gene showed resistance
to Potato virus X (PVX) (Bendahmane et al., 1999). Transgenic tomato
plants carrying the cloned N gene of tobacco show resistance to Tobacco
mosaic virus (Whitham et al., 1996). The potato Rx gene has been transferred to tobacco, and the transgenic tobacco plants show resistance to PVX
(Bendahmane et al., 1995). It may be ideal to clone the resistance genes and
exploit them to develop new disease-resistant varieties.
Great potential exists to exploit disease resistance genes for disease management, and more studies are needed in this aspect. However, the silencing
or inactivation of the transgene in disease-resistant transgenic plants poses a
problem. It is known that homologues of resistance genes exist in susceptible plants, and the interactions between an inserted gene and its DNA sequence homologues may lead to the inserted gene silencing (Jorgensen,
1990). The consequence of the interactions among the loci with DNA sequence homology may result in chromatin restructuring or DNA sequence
modification by methylation of different cytosine residues or inhibition of
mRNA processing, transport, export, or translation. The expression of the
transgene in the transgenic plant may be finally cosuppressed and transactivated. However, inactivation of the transgene may not occur in all plants.
Hence, a large number of progeny should be obtained for selecting a homozygous line with a consistently high level of resistance to the pathogens.
PLANT DEFENSE GENES
Molecular biological studies have revealed that none of the resistance genes
code for any toxic chemicals, which contribute to disease resistance. This
job is done by another group of genes called defense genes. Resistance
genes are only regulatory genes, while defense genes are the functional
genes. Defense genes are quiescent in healthy plants. They are only sleeping
genes, requiring specific signals and signal transduction systems for activation. When defense genes are activated, defense chemicals are synthesized,
and development of the pathogen is suppressed. In susceptible varieties, the
defense genes are not activated. It is now possible to clone defense genes
and make them express constitutively by placing the gene under a suitable
promoter. Thus, we can make the sleeping defense genes active constitu-
tively and make the susceptible plants resistant even in the absence of functional resistance genes.
Pathogenesis-related genes are the most important defense genes, which
encode for pathogenesis-related (PR) proteins. Numerous PR proteins have
been detected in various plants, and most of them are highly inhibitory to
fungal (Vidhyasekaran, 1997) and bacterial (Vidhyasekaran, 2002) pathogens. Several PR genes have been cloned, and transgenic plants have been
developed. These transgenic plants show enhanced disease resistance. Transgenic tomato plants constitutively expressing a PR-5 gene showed resistance to Botrytis cinerea, Oidium lycopersicum, Leveillula taurica, and
Phytophthora infestans, and the resistance was confirmed up to the T3 generation (Veronese et al., 1999). Rice plants overexpressing rice chitinase
(PR-3) gene show increased resistance against Rhizoctonia solani (Lin
et al., 1995). Datta and colleagues (1999, 2000) have exploited rice class I
chitinase (PR-3) and PR-5 genes to develop disease-resistant rice cultivars.
A rice chitinase gene (RC7) isolated from Rhizoctonia solani-infected rice
plants was introduced into indica rice cultivars IR72, IR64, IR68899B,
MH63, and Chinsurah Boro II, and the transformants showed increased tolerance to R. solani (Datta et al., 2001). Transgenic tomato plants expressing
chitinase (Logemann et al., 1994), and -1,3-glucanase (PR-2) genes (Jongedijk et al., 1995), potato plants expressing PR-10a (Matton et al., 1993)
and PR-5 (Zhu et al., 1996) genes, alfalfa plants expressing -1,3-glucanase
(Masoud et al., 1996), rose plants expressing chitinase (Marchant et al.,
1998), and tobacco plants expressing PR-1 (Alexander et al., 1993), PR-2
(Yoshikawa et al., 1993), PR-3 (Linthorst et al., 1990; Broglie et al., 1991),
PR-5 (Melchers et al., 1993), and PR-14 (lipid transfer protein LTP2)
(Molina and Garcia-Olmedo, 1997) have been developed to manage various
fungal diseases.
A PR gene from one plant could be transferred to an unrelated plant,
making the transgenic plant resistant. Transgenic wheat plants expressing
rice PR-5 gene show resistance to scab disease caused by Fusarium graminearum (Chen et al., 1999). Transgenic oilseed rape and tomato plants expressing the defensin (PR-12) gene rs-afp2 from radish showed enhanced
resistance against fungal pathogens (Parashina et al., 1999, 2000). Transgenic chrysanthemum plants expressing rice chitinase showed resistance to
Botrytis cinerea (Takatsu et al., 1999). Transgenic tomato plants expressing
an acidic endochitinase (pcht28) isolated from Lycopersicon chilense showed
resistance to Verticillium dahliae race 2 (Tabaeizadeh et al., 1999). Tomato
plants expressing the tobacco PR-9 (peroxidase) gene (Lagrimini et al.,
1993), potato plants expressing the tobacco PR-5 gene (Liu et al., 1994), cucumber plants expressing the rice chitinase gene (Tabei et al., 1998), soybean plants expressing the tobacco PR-2 gene (Yoshikawa et al., 1993), and
tobacco plants expressing rice chitinase (Zhu et al., 1993), barley chitinase
(Jach et al., 1995), sugarbeet chitinase (Nielsen et al., 1993), bean chitinase (Roby et al., 1990), peanut chitinase (Kellmann et al., 1996), barley PR-2
(Jach et al., 1995), radish PR-12 (Terras et al., 1995), barley PR-13 (thionin)
(Florack et al., 1994), barley ribosome-inactivating protein (Logemann
et al., 1992), and maize ribosome-inactivating protein (Maddaloni et al.,
1997) genes have been developed, and most of them show enhanced resistance to pathogens.
Some pathogenesis-related proteins have been shown to have antibacterial activity. These PR genes do not express constitutively. The PR genes
have been cloned, and transgenic plants constitutively expressing the PR
genes have been developed. Some transgenic plants constitutively expressing PR proteins show enhanced resistance to bacterial pathogens. Tobacco
plants expressing barley thionin genes show resistance to P. syringae pv.
tabaci (Carmona et al., 1993). Transgenic tobacco plants expressing barley
lipid-transfer proteins 2 (LTP2) show high resistance to P. syringae pv.
tabaci (Molina and Garcia-Olmedo, 1997). Similarly, transgenic Arabidopsis plants expressing barley LTP2 showed enhanced resistance to P. syringae pv. tomato (Molina and Garcia-Olmedo, 1997).
Pathogenesis-related proteins also contribute to virus-disease resistance.
Transgenic plants expressing genes responsible for activation of synthesis
of PR proteins show increased disease resistance. Transgenic tobacco plants
expressing the hrmA gene from P. syringae pv. syringae showed elevated
synthesis of PR proteins. These transgenic plants showed resistance to the
Tobacco mottling virus and the Tobacco etch virus (TEV) (Shen et al.,
2000). An antiviral protein has been isolated from pokeweed, Phytolacca
americana. Transgenic tobacco plants expressing pokeweed antiviral protein (PAP) or a variant (PAP-v) showed resistance to a broad spectrum of
plant viruses including Tobacco mosaic virus and Potato virus X. Expression of PAP-v in transgenic plants induces synthesis of pathogenesis-related
proteins. The enzymatic activity of PAP may be responsible for generating a
signal that may activate defense mechanisms against viruses (Smironv et al.,
1997). A mutant of pokeweed antiviral protein, PAPn, was nontoxic to viruses when expressed in transgenic tobacco plants. However, the tobacco
transgenic plants expressing PAPn induced basic PR proteins, proteinase inhibitor II (PR-6 protein), and protein kinase and showed resistance to viruses (Zoubenko et al., 2000).
Although several transgenic plants expressing PR genes have been developed, field performance of them has not yet been demonstrated. The increased resistance observed in the transgenic plants was also not high. Probably, a single PR protein may not be sufficient to confer resistance. Synergism
between PR proteins has been reported. Coordinated induction of the differ-
ent families of PR proteins in the same genome may indeed confer a level of
resistance considerably broader than any protein itself. Most of the phytopathogenic fungi that have been tested in vitro are resistant to -1,3glucanases and chitinases, but their growth is highly inhibited by a combination of these PR proteins. Synergism of LTPs with thionins has been
reported. Hence, transgenic plants expressing more than one PR protein
should be developed. Transfer of more genes with suitable promoters may
be difficult to achieve; scientists prefer to transfer one or two genes rather
than multiple genes. Veronese and colleagues (1999) constructed cassettes
containing three target genes, PR-1, PR-3, and PR-5 genes, used for transformation of tomato plants. One good method to transfer multiple genes has
been suggested by Zhu and colleagues (1994). They developed transgenic
tobacco plants expressing a gene encoding rice basic chitinase and transgenic tobacco plants expressing alfalfa acidic glucanase. Hybrid plants
were generated by crossing transgenic parental lines. Homozygous selfed
progeny that expressed the two transgenes could be selected from the hybrids. Logemann and colleagues (1994) could develop transgenic tomato
plants simultaneously expressing class I chitinase and -1,3-glucanase.
This type of approach will be useful in developing disease-resistant plants.
Phytoalexins are the second group of defense chemicals in plants. Overproduction and early production of phytoalexins may confer resistance in
susceptible varieties. Genes encoding phytoalexin-biosynthetic enzymes have
been cloned. Isoflavone-O-methyl transferase is an essential enzyme in
biosynthesis of the phytoalexin medicarpin in legumes. Transgenic alfalfa
plants overexpressing the enzyme show increased induction of phytoalexin
pathway transcripts after infection with Phoma medicaginis. These transgenic plants showed resistance to P. medicaginis (He and Dixon, 2000).
Resveratrol synthase is the key enzyme in biosynthesis of the phytoalexin
resveratrol in grapevine. The gene encoding this enzyme has been cloned
and transferred to tobacco. The transgenic tobacco plants showed resistance
to Botrytis cinerea (Hain et al., 1993). Transgenic rice plants expressing
grapevine stilbene synthase show resistance to Magnaporthe grisea (StarkLorenzen et al., 1997). However, the transgenic tomato plants expressing
grapevine resveratrol synthase did not show much enhanced resistance to
Phytophthora infestans. This type of specificity of phytoalexins to pathogens is well known (Vidhyasekaran, 1997). Alfalfa was transformed with
resveratrol synthase gene from grapevine. Transgenic plants accumulated a
new phytoalexin, reveratrol-3-O- -D-glucopyranoside and showed resistance to Phoma medicaginis (Hipskind and Paiva, 2000). Transgenic wheat
plants expressing a grapevine stilbene synthase gene have also been developed (Fettig and Hess, 1999).
Phenolics are another important group of toxic chemicals inhibitory to

fungal and bacterial pathogens. Overproduction of phenolics may contribute to disease resistance. Anthocyanins are one important group of phenolics.
Transgenic rice plants expressing maize anthocyanin genes showed increased blast resistance (Madhuri et al., 2001). Lignins are another important group of phenolics, and peroxidase is the important enzyme in biosynthesis of lignins. Transgenic tobacco plants expressing the peroxidase
gene Shpx2 from Stylosanthes humilis show resistance to Phytophthora
nicotianae pv. nicotianae and Cercospora nicotianae (Way et al., 2000).
Rir1b is one of a set of putative defense genes, and its transcripts accumulate
in incompatible interactions in rice. Transgenic rice plants overexpressing
this gene confer resistance to Magnaporthe grisea, the blast pathogen
(Schffrath et al., 2000).
TRANSGENIC PLANTS EXPRESSING GENES INVOLVED
IN A SIGNAL TRANSDUCTION SYSTEM
It is known that defense genes are abundant even in the susceptible varieties, but they are also quiescent in them. Specific signals are needed to activate the defense genes. Once the defense genes are activated, the susceptible
plants will become resistant. Several genes involved in the activation of a
signal transduction system have been cloned from various bacteria, fungi,
and viruses and transferred to plants. Elicitins are the elicitors produced by
Phytophthora spp. Transgenic tobacco plants expressing a Phytophthora
cryptogea gene encoding the highly active elicitor cryptogein were developed. The transgenic plants showed enhanced resistance to Thielaviopsis
basicola, Erysiphe cichoracearum, and Botrytis cinerea (Keller et al.,
1999). Harpin is a protein produced by several Erwinia species. It elicits
disease resistance in incompatible hosts. Transgenic Arabidopsis thaliana
plants expressing a harpin gene (hrpN) showed resistance to Peronospora
parasitica (Bauer et al., 1999). Transgenic potato plants expressing a harpin
protein gene from the apple blight pathogen Erwinia amylovora showed resistance to Phytophthora infestans (Li and Fan, 1999). The hrmA gene is a
component of the hrp genes in Pseudomonas syringae pv. syringae. Transgenic tobacco plants expressing the hrmA gene from P. syringae pv. syringae showed elevated synthesis of PR proteins. These transgenic plants
showed resistance to Phytophthora nicotianae var. parasitica and Pseudomonas syringae pv. tabaci (Shen et al., 2000). Pectic enzymes can form unsaturated oligogalacturonides from plant cell-wall pectin, and these galacturonates activate genes encoding phytoalexins, PR proteins, phenolics, and
cell-wall proteins. Transgenic potato plants expressing a pectate lyase gene,
PL3, of Erwinia carotovora ssp. atroseptica show enhanced resistance to E.

carotovora (Wegener et al., 1996). The PL3 gene induced the defense gene
encoding phenylalanine ammonia-lyase (Wegener et al., 1996). Polygalactunose inhibitor proteins have been detected in plant cells, and they inhibit
polygalacturonases produced by pathogens resulting in production of the
endogenous elicitors, unsaturated oligogalacturonates. Transgenic tomato
plants expressing the pear fruit polygalacturonase inhibitor protein show resistance to Botrytis cinerea (Powell et al., 2000). An antiviral protein has
been isolated from pokeweed, Phytolacca americana. Transgenic tobacco
plants expressing a variant of pokeweed antiviral protein showed increased
synthesis of PR-6 pathogenesis-related protein and protein kinase and
showed resistance to fungal pathogens (Zoubenko et al., 2000).
Salicylic acid has been shown to be an important systemic signal in
inducing resistance against pathogens. Several genes have been shown to
induce this signal. These genes have been used to develop transgenic plants
with overproduction of salicylic acid. Enhanced synthesis of salicylic acid
enhances disease resistance. Salicylic acid may be synthesized from chorismate, the general precursor of aromatic compounds. This substrate is converted
by isochromistate synthase (ICS) to isochromismate, which is subsequently
cleaved by pyruvate lyase (IPL) to yield salicylic acid. Transgenic tobacco
expressing both the ICS gene from Escherichia coli and the IPL gene from
Pseudomonas fluorescens showed constitutive accumulation of salicylic
acid. The transgenic plants showed a 500- to 1,000-fold accumulation of
salicylic acid and salicylic acid glucoside compared to control plants. These
transgenic tobacco plants constitutively expressed defense genes, particularly PR genes, and showed resistance to a fungal pathogen (Oidium lycopersicon) (Verberne et al., 2000). Transgenic potato plants expressing a
Halobacterium halobium gene encoding a light-driven bacterio-opsin proton pump showed higher systemic levels of salicylic acid and showed resistance to Phytophthora infestans (Abad et al., 1997). Transgenic tobacco
plants expressing the H. halobium gene encoding a light-driven bacterioopsin proton pump showed higher systemic levels of salicylic acid. These
plants showed enhanced resistance to the wild-fire pathogen P. syringae pv.
tabaci (Mittler et al., 1995). However, the same gene, when expressed in potato, did not offer protection against tuber infection of E. carotovora (Abad
et al., 1997). Probably, this gene would not have expressed in tubers, although it would have expressed in its leaves (the leaves of transgenic potato
plants showed resistance to the fungal pathogen Phytophthora infestans).
Abad and colleagues (1997) have used the figwort mosaic virus promoter in
the gene construction. Wegener and colleagues (1996) reported that when
they used cauliflower mosaic virus (CaMV) 35S promoter for expression
of the pectate lyase gene PL3, the expression was stronger in potato leaves
and much less intense in tubers. However, when patatin B33 was used as a
promoter, the gene expression was limited to tubers only. Different types of
promoters should probably be tested to achieve maximum expression of the
desired gene.
Transgenic tobacco plants expressing a gene encoding the A1 subunit of
cholera toxin showed increased resistance to P. syringae pv. tabaci (Beffa
et al., 1995). The cholera toxin is known to activate the signal transduction
system dependent on G proteins in animals. The transgenic plants showed
enhanced accumulation of salicylic acid (Beffa et al., 1995). H2O2 is an important component in the signal transduction system. A glucose oxidase
gene from the fungus Aspergillus niger was transferred to potato. The transgenic potato plants expressing the glucose oxidase gene showed an increased
level of resistance to E. carotovora (Wu et al., 1995). Although glucose
oxidase was produced constitutively and extracellularly in the transgenic
plants, a significant increase in H2O2 was detected only following bacterial
infection. Intracellular glucose is probably released only after bacterial infection, and the released glucose in the apoplast may serve as a substrate for
the extracellular glucose oxidase. The induced H2O2 may induce salicylic
acid and induce resistance.
H2O2 is degraded by catalase, and catalase may suppress the signal
transduction. When potato roots were infected with E. carotovora or Corynebacterium sepedonicum, a catalase gene (Cat2St) was induced. Cat2St was
found to be systemically induced after compatible interaction. Hence, suppression of a catalase gene may result in H2O2 signal transduction and induce resistance. Transgenic tobacco plants with severely depressed levels of
catalase have been developed with antisense suppression (Takahashi et al.,
1997). These plants showed both enhanced accumulation of salicylic acid
and enhanced disease resistance (Takahashi et al., 1997).
The cuticle forms an important barrier on plant surfaces against the ingress of pathogens into the hosts (Vidhyasekaran, 1997). The cuticle consists of cutin. Cutin monomers could serve as a signal to the plant during a
pathogen attack, triggering defense responses. Cutin monomers elicit H2O2
and enhance the activity of other H2O2 elicitors (Fauth et al., 1998). Cutin
monomers are derived from fatty-acid precursors. Hence, it is possible that
by releasing cutin monomers, diseases can be managed. Tomato plants were
transformed with the yeast -9 desaturase gene. In transgenic plants, large
increases in 9-hexadecenoic acid (cutin monomer) were observed, and these
plants showed increased resistance to Erysiphe polygoni. Cutin monomers
from the transgenic plants inhibited spore germination of the pathogen
(Wang, Chin, and Gianfagna, 2000).
TRANSGENIC PLANTS EXPRESSING PLANT

PROTEINASE-INHIBITOR GENES
Viral cysteine proteinases are involved in the processing and replication
of potyviruses in plants. The potyvirus genome encodes a polyprotein that is
processed by proteolysis into individual gene products. Three proteinases
have been shown to be involved in the processing mechanism, and at least
one of them seems to be of the cysteine type. An essential step for the replication of potyviruses is the activity of proteases identified as cysteine proteinases, which process the 346 kDa polyprotein. Cystatins are powerful
and specific inhibitors of cysteine proteinases. Hence, cystatins can be used
to develop virus-disease-resistant plants. Tobacco plants transformed with a
rice cysteine proteinase inhibitor (oryzacystatin) gene showed resistance to
the Tobacco etch virus and Potato virus Y (Gutierrez-Campos et al., 1999).
TRANSGENIC PLANTS EXPRESSING VIRAL GENES
Most successful transgenic plants with viral-disease resistance have been
developed by using viral genes themselves. This phenomenon is called
pathogen-derived resistance. The two most common viral genes used are
those coding for the coat protein and the RNA-dependent RNA polymerase
(also called replicase). The polymerase copies the viral genome. The coat
protein gene tends to confer resistance to a broader range of related viruses,
but resistance is often incomplete, with the plant showing delayed and
milder symptoms than the untransformed plant. Resistance can be overcome by extremely high levels of inoculum or inoculation with naked viral
RNA. In contrast, polymerase (replicase) gene-mediated resistance can
confer complete immunity, but only to virus strains with a very high sequence
homology to the one from which the transgene was derived. Besides using
these two groups of viral genes, viral movement protein genes, untranslatable sense and antisense RNAs, proteases, defective-interfering RNAs, satellites, and ribozymes have been used to develop virus-resistant transgenic
plants.
Transgenic plants expressing a coat protein (CP) gene have been reported by several researchers. Transgenic zucchini squash plants expressing
the coat protein genes of the Squash mosaic virus (SqMV) showed resistance to SqMV (Jan, Pang, and Gonsalves, 2000; Jan, Pang, Tricoli, and
Gonsalves, 2000). Transgenic Nicotiana benthamiana plants expressing the
Arabis mosaic nepovirus (ArMV) coat protein gene showed resistance to
the virus (Spielmann et al., 2000). The resistance was expressed as a delay
in infection and a reduction of the percentage of infected plants (Spielmann
et al., 2000). Transgenic tobacco plants expressing Andean potato mottle virus (APMoV) coat protein genes (CP22 and CP42) show resistance to
APMoV (Neves-Borges et al., 2001). Nicotiana benthamiana plants were
transformed with the coat protein coding sequence and the 3' nontranslated
region of the severe strain of Sweet potato feathery mottle virus (SPFMV-S),
and these transgenic plants showed resistance to SPFMV-S (Sonoda et al.,
1999). Tamarillos (Cyphomandra betacea) expressing a Tamarillo mosaic
potyvirus (TaMV) coat protein showed resistance to TaMV (Cohen et al.,
2000). Transgenic N. benthamiana plants expressing Grapevine virus A
(GVA) coat protein sequences showed resistance to the virus (Radian-Sade
et al., 2000). Transgenic tobacco plants expressing the Tobacco vein mottling virus (TVMV) coat protein gene expressed resistance against various
potyviruses and transgenic tobacco plants expressing the Alfalfa mosaic virus (AMV) coat protein gene expressed resistance against AMV (Xu et al.,
1999). Transgenic cucumber plants expressing the Watermelon mosaic virus (WMV-2) coat protein gene showed enhanced resistance to WMV-2
(Wang, Zhao, and Zhou, 2000). Nicotiana benthamiana and potato plants
expressing the coat protein gene from the Potato mop-top virus (PMTV)
showed resistance to PMTV (McGeachy and Barker, 2000). Transgenic tobacco plants expressing a Potato virus Y coat protein gene showed resistance to PVY (Han et al., 1999). Three tobacco cultivars expressing the
nucleocapsid (N) gene of a dahlia isolate of the Tomato spotted wilt virus
(TSWV) showed resistance to TSWV in the field (Herrero et al., 2000). Peanut lines transgenic for the antisense nucleocapsid (N) gene of a TSWV
strain isolated from peanut showed resistance to TSWV under field conditions (Magbanua et al., 2000). Transgenic Nicotiana tabacum and N. benthamiana plants expressing the N gene of TSWV showed resistance against
TSWV (Accotto et al., 2000). Both an RNA-mediated and a protein-mediated resistance may be acting in the transformed plants expressing nucleocapsid proteins (Accotto et al., 2000).
Viral replicase genes have been exploited in developing virus disease resistant plants. Russet Burbank potatoes have been genetically modified with
the full-length replicase gene of the Potato leafroll virus (PLRV), and these
plants showed resistance against PLRV. This transgenic plant has been patented and marketed under the name NewLeaf Plus (Lawson et al., 2001).
Wheat plants expressing the replicase gene NIb of the Wheat streak mosaic
virus (WSMV) showed resistance to WSMV (Sivamani et al., 2000). Some
transgenic clones of potato expressing Potato virus YN (PVYN) replicase
showed resistance to PVYN (Flis and Zimnoch-Guzowska, 2000). Transgenic rice plants expressing Rice yellow mottle sobemovirus (RYMV) RNA
polymerase were resistant to RYMV strains from different African locations. In these plants, virus multiplication was suppressed. The resistance
was stable over at least three generations. The viral replicase gene-mediated
resistance was also associated with posttranscriptional gene silencing (PTGS)
(Pinto et al., 1999). Nicotiana benthamiana plants transformed with a Pepper mild mottle virus (PMMoV) 54 kDa fragment of viral replicase showed
complete resistance to PMMoV (Tenllado and Diaz-Ruiz, 1999).
Nicotiana bethamiana plants were transformed with the cytoplasmic inclusion protein (CI) gene of the Plum pox virus (PPV). The CI protein is an
RNA helicase that contains a conserved nucleotide binding motif (NTBM),
and it plays an important role in viral replication. Transgenic lines containing the gene mutated in the NTBM region remained completely symptomless after Plum pox virus infection (Wittner et al., 1998). Viruses move
from cell to cell in the plant through plasmodesmata, which connects the cytoplasm between neighboring cells. Viral movement protein (MP) helps viral movement in plants. The movement proteins of plant viruses are localized to plasmodesmata in infected plant cells. They induce a significant
increase in the plasmodesmatal exclusion limit and bind single-stranded
RNA. This allows the viral genome to migrate from cell to cell. The MPs expressed in transgenic plants can complement the cell-to-cell movement of
MP-defective viral mutants. Transgenic plants expressing a viral movement
protein gene show increased susceptibility to viruses (Yoshikawa et al.,
2000). The transgenic Nicotiana occidentalis plants expressing a movement
protein of the Apple chlorotic leaf spot virus (ACLSV) showed increased
susceptibility to a homologous virus (ACLSV). However, the transgenic
plants showed strong resistance to the heterologous virus Grapevine berry
inner necrosis virus (GINV) (Yoshikawa et al., 2000). Although tobacco
plants expressing the Cucumber mosaic virus MP gene did not show resistance to CMV infection, transgenic plants expressing a deletion mutant of
the CMV MP gene showed high resistance to CMV infection (Zhang et al.,
1999). Transgenic potato lines carrying a mutant movement protein gene of
the Potato leafroll virus showed resistance to potato viruses (Rohde et al.,
2000). Transgenic tobacco plants expressing a defective MP of the Tobacco
mosaic virus, are resistant to TMV and also to viruses of other taxa, i.e., Alfalfa mosaic virus, Cucumber mosaic virus, Tobacco rattle virus, and Peanut streak virus (Cooper et al., 1995). Thus, any altered nonfunctional MP
gene confers resistance against a wide range of viruses. This phenomenon is
called MP-derived resistance.
The viral gene-induced resistance may be mostly due to posttranscriptional gene silencing. The gene silencing may be due to host-induced, sequence-specific RNA degradation. An incoming virus containing the same
sequences would also be targeted for degradation. Several studies have
proven this hypothesis. Resistance in the transgenic tobacco plants expressing the APMoP coat protein gene is mediated by transcriptional gene silenc-
ing mechanism, as there was an inverse correlation between resistance and

the accumulation of CP transgene mRNAs. The gene silencing was due to
methylation (Neves-Borges et al., 2001). When N. benthamiana plants were
transformed with the coat protein coding sequence and the 3' nontranslated
region of the severe strain of the Sweet potato feathery mottle virus, seven
out of 19 transgenic plants showed resistance to Potato virus X (Sonoda
et al., 1999). In most of the resistant lines, relatively low steady-state accumulation of the coat protein gene mRNA and few or no protein products
were observed. The regions of the transgene corresponding to the RNA target in the resistant lines were differentially methylated compared to the
transgene sequence in a susceptible line. These results suggest that the resistance in the transgenic plants is due to a posttranscriptional gene-silencing
mechanism. More convincing evidence to prove the hypothesis was presented by Jan, Pang, Tricoli, and Gonsalves (2000). Transgenic N. benthamiana plants expressing 218 and 110 base pair (bp) N gene segments of
the Tomato spotted wilt virus did not show any resistance to TSWV. But,
when the N gene segments were fused to the nontarget green fluorescent
protein (GFP) gene and N. benthamiana plants were transformed with the
chimeric genes, the transgenic plants showed resistance to TSWV. The GFP
gene is known to induce PTGS of other genes. The GFP DNA-induced
PTGS would have targeted N gene segments and the incoming homologous
TSWV for degradation, resulting in a resistant phenotype. In another experiment, it was shown that the full-length coat protein gene of the Turnip mosaic virus (TuMV) was linked to 218 N gene segments and transformed into
N. benthamiana (Jan, Pang, Tricoli, and Gonsalves, 2000). The transgenic
lines expressing the N gene of the TSWV and the coat protein gene of the
TuMV showed resistance to both viruses. The TuMV CP gene is known to
induce PTGS and would have helped the N gene of the TSWV to express
PTGS.
Gene silencing appears to be a common phenomenon of virus-disease resistance. RNA-mediated cross-protection has been reported in plants infected with nepoviruses, caulimoviruses, tobraviruses, and potexviruses.
The upper leaves of plants infected with these viruses are symptom free and
contained reduced levels of the virus. These leaves are said to be recovered.
Recovery is associated with RNA-mediated cross-protection against secondary virus infection (Ratcliff et al., 1999). A transient gene expression assay showed that RNA-mediated cross-protection was functionally equivalent to posttranscriptional gene silencing (Ratcliff et al., 1999). To uncover
factors that may play roles in gene silencing, sequences in the 3' part of the
transcribed region of the Potato virus Y coat protein gene were transcribed
in vitro, and the RNA fragments were incubated with cell extracts from
transgenic tobacco plants expressing the coat protein gene of PVY. Ribo-
nuclease activity was detected that appeared to be specific for this transcript
in the PVY-resistant transgenic plants (Han et al., 1999).
Viral genes may also code for elicitors of defense mechanisms. The disease resistance gene N has been cloned in tobacco and induces resistance
against TMV. The TMV-encoded elicitor of this gene has been identified as
TMV replicase. The TMV replicase proteins interact with the N-gene product to activate defense genes of the host (Padgett et al., 1997). The elicitor of
the disease resistance gene Rx in potato has been identified as a capsid protein of PVX (Bendahmane et al., 1995). The resistance induced by the
capsid protein was shown to be due to the elicitation of a response that was
not specific to the viral target sequence (Bendahmane et al., 1999).
Several plant viruses in the family Tombusviridae are known to generate
defective interfering (DI) RNAs during their replication. In many cases, the
viral symptoms are ameliorated by the DIs. Rubio and colleagues (1999)
designed a DNA cassette to transcribe defective interfering RNAs of the Tomato bushy stunt virus (TBSV), in which DI RNA sequences were flanked
by ribozymes (RzDI). When RzDI RNAs transcribed in vitro were mixed
with parental TBSV transcripts and inoculated into plants, they became amplified, reduced the accumulation of the parental RNA, and mediated attenuation of the lethal syndrome characteristic of TBSV infections. Transgenic
N. benthamiana plants expressing defective interfering RNAs from the
TBSV showed protection against a wide spectrum of tombusviruses but remained susceptible to a distantly related tombus-like virus and to unrelated
viruses (Rubio et al., 1999).
TRANSGENIC PLANTS EXPRESSING FUNGAL
AND BACTERIAL GENES
Transgenic Plants Expressing Fungal Genes
Chitinases are lytic enzymes which degrade chitin, a common fungal
cell-wall component. Degraded products of chitin are also elicitors of defense mechanisms in plants. Several fungi, particularly saprophytes, are
known to produce chitinases, and the genes encoding chitinases have been
cloned. Transgenic apple plants expressing genes encoding endochitinase
cloned from Trichoderma harzianum showed increased resistance to apple
scab (Bolar et al., 2000) and powdery mildew (Podosphaera leucotricha)
(Hanke et al., 1999). The gene ech-42 encoding an endochitinase from
T. harzianum was introduced alone or in combination with the osmotin gene
from N. tabacum into Petunia hybrida. The transgenic plants showed resistance to Botrytis cinerea (Esposito et al., 2000). ThEn-42, an endochitinase
gene cloned from strain P1 of T. harzianum, was introduced into the japonica rice varieties Taibei 309 and Nonghu 6. These transgenic plants expressed high resistance to the sheath blight pathogen Rhizoctonia solani
(Qin et al., 2000). Phenolics appear to play an important role in inducing resistance against virus diseases. Plants overexpressing genes coding for phenolic synthesis have shown virus-disease resistance. Tobacco plants expressing a yeast-derived invertase ( -fructofuranosidase) gene showed increased
synthesis of phenolics, particularly a caffeic acid amide (N-caffeoylputrescine). These transgenic plants showed resistance to Potato virus Y (Baumert
et al., 2001).
Transgenic Plants Expressing Bacterial Genes
Non-haem chloroperoxidases of Pseudomonas pyrrocinia catalyzes the
oxidation of alkyl acids to peracids by hydrogen peroxide. Alkyl peracids
possess potent antifungal activity. Tobacco plants were transformed with a
gene for chloroperoxidase from P. pyrrocinia. Transgenic tobacco plants
expressing this gene showed enhanced resistance to pathogens. The leaf extracts from the transgenic plants inhibited growth of Aspergillus flavus by
up to 100 percent (Jacks et al., 2000). Rajasekaran and colleagues (2000) reported that transgenic tobacco plants producing chloroperoxidase encoded
by Pseudomonas pyrrocinia showed increased resistance against Colletotrichum destructivum, the causal agent of tobacco anthracnose.
Transgenic Plants Expressing Genes from Fungal Viruses
Some double-stranded RNA viruses infect fungi. They secrete killing
proteins (KP). The Ustilago maydis-infecting virus secretes an antifungal
protein called KP4. The cDNA encoding the antifungal protein KP4 was inserted behind the ubiquitin promoter of corn and genetically transferred to
wheat varieties susceptible to stinking smut (Tilletia tritici) disease. The
transgenic plants showed increased resistance to T. tritici (Clausen et al.,
2000). The transgenic lines showed an antifungal activity against U. maydis
and the antifungal activity correlated with the presence of the KP4 transgene (Clausen et al., 2000).
Transgenic Plants Expressing Genes from Bacteriophages
Lysozymes are known to lyse bacterial cells. Several genes coding for
lysozymes have been isolated from bacteriophages. Bacteriophages lyse the
bacterial cell walls producing lysozymes. A lysozyme has been purified
from bacteriophage T4 and was active against both gram-negative and

gram-positive bacteria. The phage T4 shows lytic action against E. carotovora, and a receptor for phage T4 has been reported in the bacterium.
Attempts were made to induce disease resistance by transferring a bacteriophage T4 lysozyme gene into plants and causing it to secrete to the intercellular spaces in which the bacterial pathogens multiply. In the developed
transgenic tobacco plants, the T4 lysozyme was found to be secreted into
the intercellular spaces. The same chimeric gene cassette was transferred to
potato through Agrobacterium tumefaciens-mediated transformation. The
T4 lysozyme could be detected in intercellular washing fluids of transgenic
potato plants (During et al., 1993). The transgenic potato plants expressing
the bacteriophage T4 lysozyme showed high resistance to E. carotovora
ssp. atroseptica as shown by reduced tissue maceration in transgenic plants.
No black leg symptoms developed in transgenic potato plants until harvest,
while no control plants survived under greenhouse conditions (During et al.,
1993). Transgenic potato plants expressing the T4 lysozyme gene also
showed resistance to E. carotovora ssp. carotovora (Ahrenholtz et al.,
2000). Transgenic apple plants expressing the bacteriophage T4 gene have
been developed and show resistance to E. amylovora (Ko et al., 1999).
TRANSGENIC PLANTS
EXPRESSING INSECT GENES
Cationic antimicrobial proteins have been detected in insects. These proteins also have been exploited to develop disease-resistant plants. Cecropins
are a family of homologous antibacterial peptides derived from the giant
silk moth, Hyalophora cecropia (Mills and Hammerschlag, 1993). The
cecropins have no effect on eukaryotic cells but affect prokaryotic cells
through the formation of voltage-dependent ion channels. The lytic peptides
form pores in bacterial membranes. A chimeric cecropin B gene was introduced into Pelargonium hortorum plants. Transgenic plants showed resistance to Xanthomonas (Renou et al., 2000). Cecropins are unstable in plants
and are degraded by plant proteases. Several stable analogs of cecropin B,
including SB-37, Shiva-1, and MB39, have been synthesized. Transgenic
tobacco plants expressing Shiva-1 showed enhanced resistance to Ralstonia
solanacearum (Jaynes, 1993). Transgenic apple (Norelli et al., 1999) and
pear (Reynoird, Mourgues, Chevreau, et al., 1999) plants expressing SB-37
show resistance to Erwinia amylovora.
The stable analog of cecropin, MB39, was used to develop transgenic tobacco plants (Huang et al., 1997). A chimeric gene fusion cassette, consisting of a secretory sequence from barley -amylase joined to the MB39 cod-
ing sequence and placed under control of the promoter and terminator from
the potato proteinase inhibitor (PiII) gene, was introduced into tobacco by
Agrobacterium-mediated transformation. No disease symptom development was observed in leaf tissues infiltrated with P. syringae pv. tabaci in
MB39 transgenic plants, while in control plants disease developed rapidly.
The attacins are another class of lytic peptides isolated from the giant silk
moth. The attacin E (att E) gene has been introduced into apple plants. The
transgenic apple showed resistance to fire blight (E. amylovora) (Norelli et al.,
1994; Ko et al., 2000). Transgenic pear expressing att E (Reynoird, Mourgues,
Norelli, et al., 1999), SB-37, and Shiva-1 (Chevreau et al., 1999) showed resistance to E. amylovora.
Transgenic cauliflower plants expressing the Shiva protein showed resistance to Xanthomonas campestris pv. campestris (Braun et al., 2000). A
synthetic gene encoding a N-terminus-modified cecropin-melittin cationic
peptide (isolated from bees) chimera was introduced into two potato cultivars.
The transgenic plants showed high resistance to Erwinia carotovora (Osusky
et al., 2000). Antimicrobial peptides, sarcotoxins, isolated from the insect
Sarcophaga peregrina show severalfold higher antibacterial activity and
broader spectra than cecropin B. Transgenic tobacco plants expressing sarcotoxin IA showed resistance to E. carotovora and Pseudomonas syringae
pv. tabaci (Ohshima et al., 1999; Mitsuhara et al., 2000).
Cecropins isolated from the giant silk moth, H. cecropia, are a family of
homologous peptides of 35 to 37 residues with amphipathic N termini and
hydrophobic C termini. Cecropins inhibited only bacterial pathogens and
not fungal pathogens. Another 26-amino acid antibacterial peptide, melittin,
is produced by bees. It has a predominantly hydrophobic N terminus with an
amphipathic C terminus. A chimeric peptide, Cecropin-melittin antibiotic
peptide (CEMA), was generated, and it contains eight amino acid residues
from cecropin A and a modified melittin sequence at the C terminus. CEMA
was toxic to plants. Toxicity was reduced by modifying the N-terminus
while retaining the original a-helical character. A synthetic gene (msrA1)
encoding a N-terminus-modified cecropin-melittin cationic peptide chimera was constructed and introduced into two potato cultivars. The transgenic plants showed high resistance to Phytophthora cactorum and Fusarium solani (Osusky et al., 2000). Antimicrobial peptides, sarcotoxins,
isolated from the insect Sarcophaga peregrina show severalfold higher
antimicrobial activity than cecropin B. Transgenic tobacco plants expressing sarcotoxin IA showed resistance to Rhizoctonia solani and Pythium
aphanidermatum (Ohshima et al., 1999; Mitsuhara et al., 2000).
TRANSGENIC PLANTS
EXPRESSING ANIMAL GENES
Transgenic Plants Expressing Hen Genes
A hen-egg-lysozyme gene has been cloned, and transgenic tobacco
plants expressing this gene have been developed (Trudel et al., 1995). Extracts from transgenic tobacco plants producing hen-egg lysozyme inhibited
the growth of several species of bacteria (Trudel et al., 1995). The chly gene,
encoding lysozyme from chicken, was introduced into potato plants. The
transgenic plants showed increased resistance to the black leg disease
caused by E. carotovora ssp. atroseptica (Serrano et al., 2000). A gene encoding a lytic peptide (tachyplesin) has been isolated from the horseshoe
crab. Transgenic potato plants expressing this gene were developed, and
these plants showed reduction in tuber rot caused by E. carotovora (Allefs
et al., 1996).
Transgenic Plants Expressing Frog Genes
Antibacterial lytic peptides have also been isolated from frogs. The gene
encoding the magainin II peptide has been cloned from the African clawed
frog. The gene was modified for plant codon usage. The gene construct included a 35S promoter, the 5' leader sequence from alfalfa mosaic virus, the
signal peptide from the tobacco PR-S gene to target protein export to the
intracellular space, and a NOS terminator. In addition, the vector contained
a NPT II (NOS-NPT II-NOS) gene for selection of transgenic cells. Transgenic cauliflower plants were developed using Agrobacterium-mediated
transformation. The transgenic plants expressing frog genes showed increased resistance to X. campestris pv. campestris (Braun et al., 2000).
Transgenic Plants Expressing Human Genes
Human lysozyme is a powerful lytic enzyme degrading fungal and bacterial cell walls. A human lysozyme gene was placed under control of the constitutive CaMV 35S promoter, and the resulting expression plasmid was introduced into carrot. The transgenic plants showed strong resistance to two
fungal pathogens, Erysiphe heraclei and Alternaria dauci (Takaichi and
Oeda, 2000). Tobacco plants were transformed with a human lysozyme
gene (Nakajima et al., 1997). These transgenic plants showed reduced disease symptoms induced by P. syringae pv. tabaci in tobacco. Lactoferrin is
an iron-binding glycoprotein known to have antibacterial properties. The
expression of a human lactoferrin gene in tobacco delayed the onset of

symptoms caused by Ralstonia solanacearum from 5 to 25 days (Zhang
et al., 1996). Transgenic pear plants expressing a lactoferrin gene show resistance to the fire blight pathogen, E. amylovora (Malnoy et al., 2000).
DETOXIFICATION GENES
Pathogens produce toxins, which have been shown to be responsible for
disease symptom development. Mutants, which do not produce toxins, have
reduced virulence. Toxin-insensitive plants show enhanced resistance. The
resistance gene cloned from maize has been shown to be involved in detoxification of the toxin produced by Helminthosporium maydis (Johal and
Briggs, 1992). Eutypa lata, the pathogen of grapevine dieback, produces a
toxin called eutypine. The toxin plays a prominent role in the expression of
eutypa dieback symptoms. A protein from Vigna radiata exhibits eutypine
reductase, which degrades the toxin into a nontoxic compound. The gene
encoding the enzyme has been cloned. The VR-ERE (eutypine reducing enzyme) cDNA encodes an NADPH-dependent reductase alcohol dehydrogenase (NADP+) of 36 kDa exhibiting a high affinity toward eutypine. Expression of the VR-ERE cDNA in transformed grapevine cells conferred
resistance to the toxin (Roustan et al., 2000).
Bacterial pathogens also produce toxins. Hence, it is possible to develop
bacterial disease-resistant plants by inactivating toxins produced by bacterial pathogens. Pseudomonas savastanoi pv. phaseolicola, the bean halo
blight pathogen, produces a toxin which induces typical symptoms of the
disease in bean. The pathogen is tolerant to its own toxin, and it has been
shown to be due to the bacterial enzyme ornithine carbomyl transferase. The
corresponding gene (argK) has been cloned. Transgenic bean plants expressing argK showed high resistance to P. savastanoi pv. phaseolicola
(Herrera-Estrella and Simpson, 1995). P. syringae pv. tabaci, the wild fire
pathogen of tobacco, produces tabtoxin which is responsible for symptom
development. The gene ttr, which encodes an enzyme that inactivates the
tabtoxin, has been cloned from P. syringae pv. tabaci. The transgenic tobacco plants expressing the ttr gene show high resistance to the pathogen
(Anzai et al., 1989).
Albicidins are the toxins produced by Xanthomonas albilineans, the sugarcane leaf scald pathogen. The toxins have been shown to be important in
the disease development. The bacterium Pantoea dispersa (= Erwinia
herbicola) was found to detoxify albicidin. The P. dispersa gene (albD) for
enzymatic detoxification of albicidin has been cloned (Zhang and Birch,

1997). Transgenic sugarcane plants expressing the albD gene showed high
resistance to the leaf scald pathogen (Birch et al., 2000).
GENES ENCODING ANTIBODIES
Monoclonal antibodies raised against a virus react with various related
virus species. Antibodies can inactivate viruses. Monoclonal antibody 3-17,
which was raised against the Potyvirus Johnsongrass mosaic virus, was
shown to react strongly with 14 potyvirus species (Xiao et al., 2000). Transgenic tobacco plants expressing a single-chain variable region antibody derived from the monoclonal antibody 3-17 showed resistance to Potato virus Y
and Clover yellow vein virus (ClYVV). It suggests that one single-chain
construct can be used to protect plants from distinct potyviruses (Xiao et al.,
2000). The Tobacco mosaic virus neutralizing single-chain Fv antibody
fragment (scFv24) was targeted to the endoplasmic reticulum and integrated into the plasma membrane of tobacco cells. The transgenic plants
showed resistance to TMV infection (Schillberg et al., 2000). The results
suggest that virus diseases can be controlled by inducing the plants to produce antibodies to viruses.
PRACTICAL UTILITY OF TRANSGENIC PLANTS

IN CROP-DISEASE MANAGEMENT
Several successful reports of development of transgenic plants with disease resistance have been published recently. The important criteria for exploiting this technology are the durability of expression of these foreign
genes in the transgenic plants, absence of toxicity, and minimal environmental impact. Field evaluation of all these technologies is important. One
major objection in adoption of transgenic plants is the presence of marker
genes in the products from these plants. Several methods have been developed to limit the expression of the marker genes to the stages at which selection is made and eliminate the marker genes well before harvest. In the near
future, we can expect that this type of genetic engineering strategy will result in development of a practical strategy for efficient management of diseases in crop plants. Globally, the area of cultivation of transgenic plants is
increasing year by year. It was 1.7 million hectares in 1996, and it increased
to 11.0 million hectares in 1997, and 27.8 million hectares in 1998 (James,
1998). Already transgenic plants expressing virus resistance are commercially available in potato, tomato, soybean, and corn.
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21
Breeding
Breeding
for Disease
for Disease
ResistanceIn
Resistance
Vitro Selection
In Vitro Selection
Plant cells can be cultured in vitro and plants can be regenerated from the
cultured cells. These regenerants show variability, and some of the variable
characters are heritable. These heritable characters also include disease resistance. In vitro selections from calluses, somaclones, gametoclones, organ
cultures, and somatic hybrids have resulted in developing disease-resistant
plants. However, in vitro selection requires proper selective agents. Furthermore, gene silencing appears to be common among somaclones. Most
of the somaclonal variations are due to epigenetic changes, and DNA modifications in the somaclones are not very stable. However, some studies report stability of somaclonal characteristics for several generations. In vitro
selection can be one alternative technology for breeders to develop highyielding and disease-resistant varieties, particularly when useful genes are
not available in the germplasm for improvement of the cultivars.
WHAT IS IN VITRO SELECTION?
Plant cells, tissues, and organs can be cultured in vitro, and these cultures
can be developed into whole plants. Cells and protoplasts (cells without
walls) can be obtained by developing calluses by plating excised plant tissues from root, shoot, leaf, meristem, seed, embryo, and pollen in a specific
nutrient medium. The cultured cells and protoplasts are totipotent, developing into whole plants under defined conditions. Some organs, such as cotyledons and young leaves, can be developed through organogenesis into
whole plants without callus formation.
Populations of cultured plant cells generally contain more genetic variability than do whole plants. Some of these variations may occur due to preexisting mutations in cells of the explant material. However, most of the
variations are due to mutations induced in the cell culture. Plant-growth regulators such as 2,4-D and kinetin used in tissue-culture media may act as
mutagens. Plants regenerated from cell, tissue, and organ cultures are called
somaclones. Vast variations are seen among somaclones as they are developed from the mutated cultured cells. Many of the variations have been
found to be stable and heritable. The stable and heritable variation displayed
among somaclones is called somaclonal variation. Somaclonal variation
occurs more frequently than the spontaneous mutations occurring in fieldgrown plants. Variation has been detected in the progeny of up to 15 percent
of regenerated plants in contrast to spontaneous mutation rates of one in one
million. Somaclonal variations have been exploited to develop diseaseresistant varieties.
TYPES OF IN VITRO SELECTION
In Vitro Selection Among the Regenerants
In this technique, thousands of calluses are developed from different
explants (whole seed, embryo, root, stem, and leaf) or cultured plant cells or
protoplasts. All the developed calluses are transferred to a regeneration medium. Some organ cultures are also regenerated, and all the regenerated
plants are tested for their reaction to pathogens. By this method, somaclones
showing resistance to various fungal, bacterial, and viral pathogens could be
selected in various crops. The following somaclones have been selected: in
rice, somaclones resistant to the blast pathogen Pyricularia oryzae (Araujo
et al., 1999) and the brown spot pathogen Helminthosporium oryzae (Ling
et al., 1985); in wheat, somaclones resistant to Karnal bunt and powdery
mildew (Sharma et al., 1998); in apple, somaclones resistant to fire blight
(Chevreau et al., 1998); in potato, somaclones resistant to late and early
blights (Matern et al., 1976) and soft rot (Erwinia carotovora ssp. carotovora) (Polgar et al., 1999); in peppermint, somaclones resistant to verticillium wilt (Sink and Grey, 1999); and in tomato, somaclones showing resistance to wilt caused by Fusarium oxysporum f. sp. lycopersici (Shahin and
Spivey, 1986), Cucumber mosaic virus (Hanus-Fajerska et al., 2000), and
bacterial wilt caused by Ralstonia solanacearum (Mandal, 1999).
Resistant plants have been selected by exploiting somaclonal variation in
many other crops. Rust (Puccinia arachidis) and late and early leaf spots
(Mycosphaerella spp.) are the most important diseases in groundnut, and
somaclones showing resistance to these diseases have been identified (Eapen
et al., 1997). The most serious disease worldwide in banana is Sigatoka leaf
spot caused by Mycosphaerella musicola. Two groups of researchers could
select resistant banana plants exploiting somaclonal variation (Trujillo
et al., 1999; Vidal et al., 2000). Somaclone resistant to Fusarium wilt of banana has also been obtained (ChingYan et al., 2000).
In Vitro Selection at the Callus Stage

Although somaclonal variation can be identified among regenerants, it is
a difficult task to achieve. Each regenerant must be individually tested for
agronomic traits under greenhouse or, preferably, under field conditions.
Similarly, each regenerant must be tested for its reaction to diseases by inoculating spores of individual pathogens and providing a suitable environment
(temperature, humidity, dew, etc.) for disease development. In the case of
viral diseases, transmission of viruses by suitable vectors should be tried.
There will be many escapes in the plant population if the pathogen inoculations were not done scrupulously. Great skill is needed to score individual
plants. Furthermore, most of the somaclones show only reduced disease intensity (reduction in lesion size, lesion number, intensity of sporulation,
etc., otherwise called quantitative resistance rather than qualitative resistance). Scoring individual plants for quantitative resistance is very difficult.
To overcome this difficulty, selection of somaclones can be done at the callus stage itself. Specific selective agents can be used to discriminate the calluses, which may yield somaclones with good agronomic traits and disease
resistance.
Calluses from susceptible plants are profusely colonized by pathogens,
while colonization in the calluses from resistant plants is significantly decreased in many host-pathogen interactions. Hence, this characteristic can
be used to select pathogen-resistant calluses. Oilseed rape callus tissues
were inoculated with Phoma lingam spores and embryogenic calluses,
which were free of attack, were selected. The regenerants showed moderate
resistance to the pathogen (Sacristan, 1982). Sun and colleagues (1986) inoculated 365 calluses from a susceptible rice variety with the bacterial
blight pathogen Xanthomonas oryzae pv. oryzae. Most of the calluses died
as the result of bacterial infection. However, 63 calluses showed sectional
proliferation. From those calluses, 45 plants could be regenerated. All but
one were resistant to the disease.
Many fungal and bacterial pathogens produce toxins, and these toxins
have been shown to be involved in disease development. Toxin-insensitive
plants show less susceptibility or even high resistance. Toxin-deficient mutants are less virulent or are avirulent. When calluses that show resistance to
toxins are regenerated, the regenerants show increased resistance to pathogens, which produce those toxins. Vidhyasekaran and colleagues selected
rice somaclones showing resistance to Helminthosporium oryzae based on
their insensitivity to the host-specific toxin produced by the pathogen
(Vidhyasekaran et al., 1986; Ling et al., 1985; Vidhyasekaran et al., 1990).
Toxin-resistant calluses were selected, and regenerants from those calluses
were tested for their susceptibility to the pathogens. Some of the regenerants
showed stable and heritable resistance to diseases. By this method, the following disease-resistant plants have been developed: wheat plants resistant
to Helminthosporium sativum; potato plants resistant to Fusarium oxysporum and Phytophthora infestans; tomato plants resistant to Alternaria
solani, Phytophthora infestans, and Fusarium oxysporum f. sp. lycopersici;
maize plants resistant to Helminthosporium maydis and Phyllosticta maydis; sugarcane plants resistant to Helminthosporium sacchari; alfalfa plants
resistant to F. oxysporum f. sp. medicaginis; oat plants resistant to Helminthosporium victoriae; barley plants resistant to H. sativum; tobacco plants resistant to Pseudomonas syringae pv. tabaci and Alternaria alternata; and
peach plants resistant to Xanthomonas campestris pv. pruni (Vidhyasekaran, 1990, 1993). The selection of disease-resistant plants using fungal
and bacterial toxins was not significantly different from the selection without any toxin. Both of these methods select only the mutants generated in
tissue-culture medium.
In Vitro Selection from Organ Cultures
Several somaclonal variations have been reported among somaclones
obtained from organ cultures without callus formation. Somaclonal variants
of groundnut having field tolerance to rust and leaf spot diseases were obtained from shoot-tip regenerants (Eapen et al., 1997). Four somaclonal
variants regenerated from adventitious buds of the apple variety Greensleeves (susceptible to fire blight) showed resistance to the fire blight caused
by Erwinia amylovora (Chevreau et al., 1998). Some of the tomato somaclones, obtained through adventitious organogenesis initiated on leaf explants
of cultivated tomato, showed increased tolerance to the cucumber mosaic
virus (Hanus-Fajerska et al., 2000).
In Vitro Selection from Somatic Hybrids
Protoplast fusion among otherwise sexually incompatible species facilitates transfer of genes from widely divergent species. This technique helps
to produce somatic hybrid plants. Somaclonal variations have also been observed among somatic hybrids. Sinapis alba is resistant to Alternaria
brassicae, which causes a serious disease in cabbage (Brassica oleracea).
Somatic hybrids between S. alba and B. oleracea were generated for transferring resistance against A. brassicae to B. oleracea (Hansen and Earle,
1997). Twenty-seven plants were regenerated from protoplast fusion. Some
of the plants obtained from cuttings from the somatic hybrids showed a re-
sistance to A. brassicae that was similar to that found in S. alba (Hansen and
Earle, 1997).
Three somatic hybrid lines between potato (Solanum tuberosum) and
Solanum brevidens were obtained (Polgar et al., 1999). The lines originated
from the same callus showed different reactions to the soft rot pathogen
Erwinia carotovora ssp. carotovora, and some of them showed less susceptibility to the pathogen (Polgar et al., 1999). The variations might have occurred in the early callus stage of development.
In Vitro Selection Exploiting Gametoclonal Variation
Anther culture-derived plants are called gametoclones and are haploid.
Gametoclonal variations have also been observed in many plants. Blast disease-resistant gametoclones have been identified in rice (Chawla and Wenzel,
1987). Some wheat gametoclones have shown resistance to powdery mildew (Vidhyasekaran, 1993).
STABILITY AND HERITABILITY OF RESISTANCE
EVOLVED THROUGH IN VITRO SELECTION
Somaclonal variations have been shown to be unstable after two to three
generations with few exceptions. In sugarcane, 73 percent of the Helminthosporium sacchari-resistant regenerants showed resistance even up to five
generations, while others reverted to being susceptible (Larkin and Scowcroft, 1983). Helminthosporium oryzae-resistant rice regenerants showed
resistance up to the R3 generation (Vidhyasekaran et al., 1990). Somaclonal
variant CIEN BTA-03 resistant to yellow Sigatoka (Mycospherella musicola) was obtained from a susceptible banana clone. This somaclone exhibited the disease resistance in the field for five consecutive years of asexual
reproduction (Vidal et al., 2000).
The inheritance pattern of resistance obtained through somaculture has
been studied in tomato (Shahin and Spivey, 1986). Fusarium wilt-resistant
plants were regenerated from protoplast-derived calluses. Analysis of R1
progenies obtained from self-fertilization of selected R0 individuals showed
a typical 3:1 (resistance:susceptible) ratio of segregation. Analysis of R2
progenies showed that resistant plants were either homozygous or heterozygous dominant for the gene conferring resistance. Out of 23 R1 plants, six
were homozygous mutants, six were homozygous normal (susceptible), and
11 were heterozygous, suggesting a theoretical ratio of 1:2:1 and confirming that the original regenerated plant was heterozygous for a dominant resistance allele. Dominant-gene mutation has been shown in tissue-culture-
derived rice plants to Helminthosporium oryzae (Ling et al., 1985). The

wilt-disease resistance observed in tissue-culture derived alfalfa plants also
has been reported to be due to mutation at a single dominant gene (McCoy,
1987). The blast-disease resistance observed in three rice somaclones appears to be controlled by a single gene (Araujo et al., 1999). DNA polymorphisms were detected in somaclones derived from somatic hybrids
(Solanum tuberosum and Solanum brevidens). Loss of certain DNA segments on chromosomes 5, 6, 9, and 11 in the somaclones has been observed
(Polgar et al., 1999).
Studies conducted so far indicate that somaclonal variation may be due to
cytological abnormalities, frequent qualitative and quantitative phenotypic
mutation, sequence change, and gene activation and silencing (Kaeppler
et al., 2000). Activation of quiescent transposable elements and retrotransposons has been observed in somaclones. It indicates that epigenetic
changes may occur through the culture process. Epigenetic activation of
DNA elements further suggests that epigenetic changes may also be involved in cytogenetic instability through modification of heterochromatin,
and as a basis of phenotypic variation through the modulation of gene function. DNA methylation patterns are highly variable among regenerated
plants and their progeny. It suggests that DNA modifications are less stable
in somaclones than in seed-grown plants (Kaeppler et al., 2000).
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production by Helminthosporium oryzae. Phytopathology, 76:261-266.
Vidhyasekaran, P., Ling, D. H., Borromeo, E. S., Zapata, F. J., and Mew, T. W.
(1990). Selection of brown spot-resistant rice plants from Helminthosporium
oryzae toxin resistant calluses. Ann Appl Biol, 117:515-523.
22
Chemical
Chemical
ControlBacterial
ControlBacterial Diseases
Diseases
Many fungicides have been developed, and several chemical industries
have invested several millions of dollars in developing fungicides. More
than 100 active fungicide ingredients have been registered, and several hundred fungicide formulations are available in the market. In contrast, very
few bactericides are available to control bacterial diseases (Research Information, 1998). In this chapter, the bactericides available in the market are
listed and their uses are given.
COMMON AND TRADE NAMES
OF THE BACTERICIDES AND THEIR USES
The following is the list of common name, trade name, and uses of
bactericides:
Ammoniacal copper sulfateCopac E, controls bacterial diseases in
pears, stone fruit, vegetables, and ornamental plants.
Bordeaux mixtureComac, Bordeaux 13 percent, Bordeaux Mixture 13
percent, Bordeaux Mixture 20 percent, Bordeaux Mixture 27 percent,
Bouillie Bordelaise RSR, mixture of copper sulfate and lime (calcium
hydroxide). Copper sulfate (10 lb) is added to 50 gallons of water, and
then this solution is added to another 50 gallons of water containing 10
lb of calcium hydroxide. This mixture must be prepared freshly before
application in the field. Ready-formulated Bourdeaux mixture is also
available; however, these formulations are inferior to freshly prepared
Bordeaux mixture. Bordeaux mixture controls fire blight of pear. It is
recommended to apply as a spray at a concentration of 1-1-100 (copper
sulfate lb-calcium hydroxide lb-water gallon), and the spray should be
applied at five- to seven-day intervals. It is applied at 4-4-50 concentration to control angular leaf spot (Pseudomonas syringae pv. lachrymans) of cucurbits. It controls citrus canker, bacterial wilt of pepper,
bacterial gummosis and canker (P. syringae), wild fire of tobacco, and
bacterial leaf spots of tomato.
Bordeaux pastepaste prepared by dissolving 1 lb copper sulfate in 3 qt

water, then mixing with 1.5 lb calcium hydroxide in 3 qt water. Bordeaux paste is used to control crown gall of apples, peaches, almonds,
apricots, avocados, cherries, nectarines, and pecans. Diseased areas are
cut away and the cut ends are covered with the paste.
BronopolBronotak, antibacterial seed treatment for control of black arm
and bacterial blight of cotton.
ChloropicrinChlor-O-Pic, preplant soil fumigant for control of soilborne bacterial pathogens.
Copper hydroxideKOH-Hydroxide, KOP Oxy, Kocide, controls bacterial diseases of citrus, apples, pears, vegetables, and ornamentals; controls bean bacterial blight (Xanthomonas axonopodis pv. phaseoli) and
halo blight (Pseudomonas savastanoi pv. phaseolicola), cabbage black
rot (X. campestris pv. campestris), cucumber angular leaf spot (P.
syringae pv. lachrymans), fire blight of pears (Erwinia amylovora),
walnut blight (X. arboricola pv. juglandis), tomato bacterial speck (P.
syringae pv. tomato), bacterial spot of peppers (X. vesicatoria), and
bacterial spot of peaches and nectarines (X. arboricola pv. pruni).
Copper oxychlorideCobox, Rokkol 400 SC, controls bacterial diseases
in fruit trees and vegetables; controls fire blight of apples and pears (E.
amylovora), walnut blight (X. arboricola pv. juglandis), tomato bacterial spot (X. vesicatoria), and angular leaf spot (P. syringae pv.
lachrymans) and bacterial wilt (Erwinia tracheiphila) of cucumbers.
Copper oxychloride sulfateC-O-C-S, a mixture of basic copper sulfate
and basic copper chloride, controls fire blight of apples and pears (E.
amylovora), walnut blight (X. arboricola pv. juglandis), tomato bacterial spot (X. vesicatoria), and angular leaf spot (P. syringae pv.
lachrymans) and bacterial wilt (Erwinia tracheiphila) of cucumbers.
Copper sulfate tribasicKOP 300, controls bacterial diseases in fruit
trees, nuts, and vegetables; controls bean bacterial blight, cucurbit
angular leaf spot, pepper bacterial spot, tomato bacterial spot, and celery bacterial spot (P. syringae pv. apii).
DodineSyllit, controls bacterial spot of peaches (X. arboricola pv.
pruni).
Flumequineeffectively controls fire blight of apples and pears.
Fosetyl-AlAliette, controls Erwinia amylovora on pears.
Galltrol (Agrobacterium radiobacter)biocontrol product, controls
crown gall in apple, pear, peach, almond, apricot, plum, cherry, blueberry, kiwi, prune, bushberry, walnut, pecan, and nectarine.
GentamicinAgri-gent, controls bacterial diseases caused by
Agrobacterium tumefaciens and Xanthomonas campestris.
Hypochloritecalcium chlorohypochlorite (Perchloran, HTH) and sodium hypochlorite (Chlorox, Purex) formulations are available, used as
surface disinfectants of plant propagative material such as grape and
rose cuttings; the cuttings for rootings and rose stems are treated 20
minutes in 0.5 percent hypochlorite solution to eliminate Agrobacterium
tumefaciens that may be carried on the surface (Schroth and McCain,
1991).
KasugamycinKasumin, controls bean halo blight (Pseudomonas
savastanoi pv. phaseolicola) and potato soft rot (Erwinia carotovora);
also controls Xanthomonas and Clavibacter diseases.
Kasugamycin + copper oxychlorideKasuran, controls citrus canker.
OcthilinonePancil-T, RH-893, controls bacterial diseases of top fruit
and citrus bacterial canker.
Oxine-copperQuinondo, controls various bacterial diseases.
OxytetracyclineTerramycin, controls fire blight of pear, bacterial spot
of peach, a tree injection formulation is available in the control of eastern X disease of peach, cherry, and nectarine. Trees are treated by gravity infusion or by pressure treatment with oxytetracycline (1,320 ppm)
using 950 ml per tree after harvest but before leaf fall (Schroth and
McCain, 1991).
ProbenazoleProbenazol, Oryzemate, controls rice bacterial blight.
Quaternary ammonium compoundsBionol, Conide, Consan, Culsan,
Dichloran, Germital, Hyamine, Onyxide, Physan, Risosan, Roccal,
Shield, Sterosept, cuttings and rootings are treated with this compound
to eliminate bacterial pathogens.
QuintozeneBrassicol, Tubergran, Terraclor, Folosan, Tritisan, Saniclor,
Avicol, Kobutol, Pentagen, Botrilex, Kobu, Earthcide, Turfcide,
RTUPCNB, PCNB, Quintozene, Tilcarex, TriPCNB, controls potato
scab caused by Streptomyces scabies.
Salicylic acidseveral formulations, induces systemic resistance and reduces disease incidence; effective against Xanthomonas oryzae pv.
oryzae.
StreptomycinAgrimycin 17, Agri-strep, Plantomycin, controls soft rot
and black leg of potato, fire blight of apple and pear, wild fire of tobacco, bacterial wilt of chrysanthemum, bacterial blight of celery, bacterial spot of tomato, black arm of cotton, rice bacterial blight, and citrus canker; controls Xanthomonas, Erwinia, Ralstonia, Pseudomonas,
and Agrobacterium diseases.
SulfurThat Big 8, Top Cop, reduces bacterial diseases.
Sulfur flowableThat Flowable Sulfur, has antibacterial action; reduces
bacterial diseases.
TecloftalamShirahagen-S, systemic bactericide; controls rice bacterial

blight.
Tetracyclinecontrols bacterial diseases.
TrichlamideHataclean, controls potato scab (Streptomyces scabies).
2,4-Xylenol + meta-cresolGallex, controls crown gall and olive knot
(Pseudomonas savastanoi) on apple, pear, peach, almond, apricot,
plum, cherry, blueberry, kiwi, prune, bushberry, walnut, pecan, and
nectarine.
EFFECTIVENESS OF BACTERICIDES
Although a few bactericides are available, their uses in management of
bacterial diseases are limited. Their efficacy in the field varies widely. Most
of the bacterial pathogens are systemic, but most of the bactericides are surface protectants. Bacteria also develop resistance to antibiotics very quickly.
Persistence of antibiotics in plants is very low, requiring that antibiotics be
applied once every four to five days. This is impractical and will be uneconomical. The current bactericides cannot reach sites where bacteria overwinter, such as blighted wood, cankers, and lesions in the case of woody
plants. Only partial control of bacterial diseases is achievable with the available bactericides.
REFERENCES
Research Information, Ltd. (1998). International Pesticide Directory, Sixteenth
Edition. Research Information Ltd., Herts, U. K.
Schroth, M. N. and McCain, H. D. (1991). The nature, mode of action, and toxicity
of bactericides. In D. Pimentel (Ed.), CRC Handbook of Pest Management in
Agriculture, Volume II. CRC Press, Boca Raton, Florida, pp. 497-505.
23
Chemical
Chemical
ControlFungal
ControlFungal Diseases
Diseases
Fungicides are the most important components in the management of
fungal diseases. More than 100 fungicides have been developed, and several
hundreds of fungicide formulations are available. They have been formulated as dustable powders, wettable powders, emulsifiable concentrates,
flowables, liquids, granules, and gases. Special formulations have also been
developed specifically for seed treatment and soil application. Formulations
consist of several adjuvants in addition to the active ingredients. Formulations of mixtures of fungicides are also available. The mode of action of different classes of fungicides varies widely, and each class of fungicides controls different groups of diseases. Specificity of fungicides appears to be
restricted to the fungal genera level, not the species level. Various types of
application equipment are available to apply fungicides to crops. Fungi develop resistance to certain fungicides, and several techniques have also been
developed to manage fungicide resistance in pathogens. All of these issues
are discussed in this chapter.
HISTORY OF CHEMICAL CONTROL
Among the various methods of disease management, chemical methods
appear to be the most important. Many farmers believe that control of crop
diseases is possible only by using fungicides, as they reduce diseases dramatically. Chemical control of diseases has been attempted since the occurrence of diseases became known. There is a mention of the use of a fungicide in the Indo-Aryan Vedas, which date from perhaps around 1500 B.C.
Around 1000 B.C., the Greeks were applying practical chemical control
against diseases. The Roman Caius Plinius Secundus (23 to 79 A.D.), in his
writings Historia Naturalis, described treatment of cereal seeds with wine
or a concoction of cypress leaf extract to control mildew. In his text
Pelzbuch, Gottfried von Franken, a German who lived around 1230 A.D.,
gave advice on treatment of cankers of cherry and some other fruit diseases.
William Forsyth (1737-1804), from Scotland, published a book in 1802
in which he recommended urine and lime-water treatment to control mildew
of fruit trees. He also stated that decoction of tobacco, sulfur, unslaked lime,
and elder buds (Sambuscus) in boiling water controls powdery mildew.
Benedict Prevost (1755-1819), in a monograph published in 1807, recommended the use of copper sulfate as a seed treatment to control bunt disease
of wheat. He demonstrated that at a concentration of 1:10,000 copper sulfate killed the fungus, without damaging the seeds. Thomas Andrew Knight
(1759-1838) in 1817 reported that sulfur controls scab (Venturia pirina) in
pear trees. John Robertson (1824) from Ireland showed that mildew (Sphaerotheca pannosa var. persicae) is controlled by a mixture of sulfur in soapsuds, the soap acting as a spreader and sticker. In 1833, William Kenrick
(1789-1872) from the United States published The New American Orchardist, in which he advocated the use of sulfur and quick lime, mixed in boiling
water and diluted for use with cold water, to control diseases. Knight (1842)
also showed that application of lime and sulfur controlled peach leaf curl
(Taphrina deformans).
Several fungicides have been developed out of necessity. In 1848, the
vine industry in France was severely affected because of outbreak of powdery mildew (Uncinula necator) in grapevine. In 1855, a fine form of sulfur
was produced to control grape powdery mildew (McCallan, 1967). Later,
downy mildew became severe in grapevine. The most important discovery
in the field of chemical control is that of Bordeaux mixture in 1885. Vine
growers in Bordeaux, France, were having problems with people stealing
grapes from different parts of the vineyard, which bordered a road. They
sprayed a mixture of copper sulfate and hydrated lime over the vines to deter
people from stealing. This spray gave the grapes a blue color and reduced
the amount of theft. Pierre Millardet, a professor working at the University
of Bordeaux, observed that vines treated with this mixture of chemicals
were almost free from downy mildew. In 1885, Millardet demonstrated the
efficacy of this mixture, called Bordeaux mixture, in the control of grapevine downy mildew in a field experiment. Since then, Bordeaux mixture has
become the most used fungicide, and it is still in use today, even after more
than 100 years, for the control of fungal diseases on a wide range of crops.
Organomercurial compounds were developed for seed treatment in the
early 1900s. The first organomercurial seed treatment for control of bunt of
wheat was introduced by E. Riehm (1914). The Bayer company from Germany introduced the first commercial organomercurial fungicide, Uspulam.
Subsequently, Imperial Chemical Industries (ICI) (U.K.) developed the
organomercurial fungicides Ceresan in 1929 and Agrosan G in 1933. Mercurial fungicides were popular for more than five decades, and now many
countries have banned their use because of environmental pollution problems. In 1934, the DuPont Company discovered the first organic fungicides,
the dithiocarbamates. Several dithiocarbamates such as zineb, maneb, man-
cozeb, and thiram were introduced within a span of another ten years. All of
these sulfur, copper, mercury, and dithiocarbamate fungicides are protectants or surface fungicides. They do not penetrate plants and cannot cure
disease. They are also subject to weathering.
In the 1960s, a new group of fungicides appeared which could enter
plants and be translocated to different parts of plants. Called systemic fungicides, they also had curative action. Their discovery revolutionized the
chemical control approach, and a new era had begun. Several chemical
companies in the United States, United Kingdom, Germany, Switzerland,
France, and Japan introduced numerous fungicides during the next 30 years.
Several ready-formulated fungicide mixtures have also been released.
DEVELOPMENT OF COMMERCIALLY VIABLE FUNGICIDES
Several commercial companies are involved in the development of fungicides. Numerous compounds are screened to select compounds with fungicidal action. The selected compounds are tested against a wide range of
pathogens in the laboratory, particularly against economically important
pathogens affecting crops of high commercial value and causing heavy
losses in those crops cultivated in vast areas. When thousands of chemicals
are screened, a large number of chemicals with some fungitoxicity can be
identified. These compounds undergo stringent secondary tests which identify the concentration at which the compound loses its efficacy, compared to
a suitable standard fungicide. The compound should be active at a low concentration (normally at 10 to 25 ppm) to merit elevation to the next stage.
Further tests include laboratory and greenhouse studies to assess the efficacy of the selected compounds against test diseases in the appropriate
hosts. Normally, these tests will be initially conducted with a single target
pathogen (most important pathogen), and then the selected compound will
be tested against other taxonomically related pathogens.
Many more tests are required to develop a compound as a commercial
fungicide. The selected compounds should have a broad disease-control
spectrum and an extended control period. Their mobility after application in
the plant should be assessed. The curative properties of the compounds
should also be explored. The absence of curative activity is a disadvantage
unless systemicity or the potential to redistribute in the crop is demonstrated. Immobile protectant activity alone may limit the use of the candidate chemical. The product should be compatible with other products, and
the formulations should be easy to use. The selected chemical should be
safe to the crops (without phytotoxicity). Several fungi are known to develop resistance to fungicides. Antiresistance activity of the selected chemi-
cals should be tested. The selected chemical should be cost effective, the
dosage required to control diseases should be low, and it should require
fewer treatments per season.
The candidate chemical should be safe to users and consumers of the
treated product and be environmentally acceptable (Waxman, 1998). The
following toxicological data are required for the registration of a fungicide:
acute oral toxicity, acute dermal toxicity, acute inhalation toxicity, primary
eye irritation, dermal sensitization, acute delayed neurotoxicity, subchronic
oral toxicity, subchronic dermal toxicity, subchronic inhalation toxicity,
subchronic neurotoxicity, chronic toxicity, oncogenicity, reproduction, teratogenecity, mutagenecity, metabolism, and pharmacology.
All of these tests are very expensive. It has been estimated that one out of
about 120,000 compounds may satisfy all these requirements and be developed as a commercial product. The average cost of developing one new synthetic compound is approximately 200 million dollars, and it takes about
eight years to launch a product. Two-thirds of the total cost is attributed to
biological efficacy trials, and toxicological and environmental safety tests
alone may account for 60 percent of this cost. An estimated 570 million dollars is spent annually on research and development by the leading 15 agrochemical companies (Knight et al., 1997).
Several chemical industries in different parts of the world are involved in
the development and marketing of fungicides. Novartis (Ciba-Geigy and
Sandoz), Bayer, Rhone-Poulenc, BASF, DuPont, AgrEvo, Rohm and Haas,
Ishihara, and Zeneca (ICI) are the major suppliers of fungicides in the
world. Their sales of fungicides exceed $4 billion annually. Mancozeb, copper,
chlorothalonil, sulfur, propiconazole, tebuconazole, metalaxyl, epoxiconazole, benomyl, iprodione, cyproconazole, maneb, carbendazim, tricyclazole,
prochloraz, oxadixyl, vinclozolin, flusilazole, fosetyl-Al, cyproconazole, thiophanate-methyl, edifenphos, thiabendazole, fenpropimorph, procymidone,
bayleton, penconazole, triflumizole, triphenyl tin, and fenbuconazole are
the major active ingredients sold in the market (Hewitt, 1998). Their sales
alone exceed $3.5 billion annually.
Common, Chemical, and Trade Names of Fungicides
The common names, chemical names, and trade names of fungicides
available in the world market are given in the following list. The common
names are followed by chemical names (in parentheses) and trade names of
each fungicide.
anilazine (2,4-dichloro-6-(2-chloroanilino)-1,3,5-triazine)B622,
Botrysan, Direz, Dyrene, Kemate, Triasyn, Direx, Zinochlor, Triazine
azoxystrobin ( -methoxyacrylate)Amistar, ICIA5504, Quadris,
Abound, Bankit, Heritage, Ortiva
benalaxyl (methyl N-phenylacetyl-N-2,6-xylyl-DL-alaninate)Galben,
M9834, Tairel, Trecatol
benodanil (2-iodobenzanilide)Calirus, BAS3170F
benomyl (methyl 1-(butylcarbamoyl) benzimidazol-2 yl carbamate)
Benlate, Benex, Fundazol, Fibenzol, Benosan, Hockley Benomyl
binapacryl (2-sec-butyl-4,6-dinitrophenyl 3-methylcrotonate)Acricid,
Endosan, Morocide, Ambox, Dapacryl, Morocid, Hoe2784
biphenyl (1,1'-biphenyl)Lemonene, PhenadorX, Phenylbenzene
bitertanol (1-(biphenyl-4 yloxy)-3,3-dimethyl-1-(1H-1,2,4-triazol-1 yl)
butan-2-ol)Baycor, Baymat, Sibutol, Biloxazol, Bay KWG 0599
blasticidin S (antibiotic)Bla-S
Bordeaux mixture (mixture of copper sulfate and calcium hydroxide)
Comac, Bordeaux 13 percent, Bordeaux Mixture 13 percent,
Bordeaux Mixture 20 percent, Bordeaux Mixture 27 percent,
Bouillie Bordelaise RSR
bronopol (2-bromo-2-nitro-1,3-propanediol)Bronocot, Bronotak
bupirimate (5-butyl-2-ethylamino-6-methylpyrimidin-4-yl
dimethylsulfamate)Nimrod, Nimrod T, PP588
buthiobate (butyl 4-tert-butylbenzyl N-(3-pyridyl) dithiocarbonimidate)
Denmert, S1358
captafol (cis N-[(1,1,2,2-tetrachloroethyl)thio] 4-cyclohexene-1,2dicarboximide)Difolatan, Haipen, Ortho5865, Merpafol, Pillartan,
Sanspor
captan (N-[(trichloromethyl)thio]-4-cyclohexene-1,2-dicarboximide)
Orthocide, Pillarcap, Merpan, Vondcaptan, Captan 50, Captanex,
Captaf, Drexel
carbendazim (methyl benzimidazol-2-ylcarbamate)Bavistin, BAS 346F,
Derosal, Battal, Focal, Hoe 17411, Delsene, Stempor, Derroprene,
Equitdazin, Kemdazin, Virolex, Lignasan, Pillarstin, Custos, Triticol
carboxin (5,6-dihydro-2-methyl-1,4-oxathiine-3-carboxanilide)Vitavax,
Kemikar, D 735, Kisvax, Vitavax 34, Enhance, ProGro, Vitavax 30C
carpropamid (1RS,3SR)-2,2-dichloro-N-[1-(4-chlorophenyl)ethyl]-1ethyl-3-ethylcyclopropane carboxamide)Win, KTU 3616
chloranil (tetrachloro-p-benzoquinone)Spergon
chloroneb (1,4-dichloro-2,5-dimethoxybenzene)Terraneb, Demosan
chloropicrin (trichloronitromethane)Chlor-O-Pic, TriChlor,
Dojyopicrin, Dolochlor, Acquinite, PicClor
chlorothalonil (2,4,5,6-tetrachloroisophthalonitrile)Bravo 500, Bombardier, Daconil 2787, Exotherm Termil, Clortocaffaro, Notar, Faber,
Repulse, Tuffcide, Clortosip, Kavach
chlozolinate (ethyl 3-(3,5-dichlorophenyl)-5-methyl-2,4-dioxooxazolidine-5-carboxylate)Serinal, Manderol, M 8164
copper hydroxide (dicopper trihydroxide)Kocide 101
copper oxychloride (copper chloride oxide hydrate)Blitox, Coprantol,
Coptox, Cupravit, Cuprocaffaro, Cuprokylt, Cobox, Fytolan, Kauritil,
Recop, Vitigran, Cuprosan, Viricuivre, Cekuper
copper sulfate (copper sulfate)Comac Bordeaux Plus, Triangle, Blue
Viking, Vencedor, Sulfacop, Phyton27
cufraneb (dithiocarbamate complex containing copper, manganese, iron,
and zinc)Cufram Z
cuprous oxide (dicopper oxide)CopperSandoz, Cobre Sandoz, Kupfer
Sandoz, Copper Nordox, Perenox, Yellow Cuprocide, Caocobre,
FungiRhap
cycloheximide (4(2 R)-2[(1S, 3S, 5S)-(3,5-dimethyl-2-oxocyclohexyl)]-2hydroxyethyl) piperidine-2,6-dione)Actidione, Actispray, Hizarocin
cymoxanil (1-(2-cyano-2-methoxyiminoacetyl)-3-ethylurea)Curzate,
DPX 3217
cyproconazole (1,2,4-triazole)Alto, Atemi, Sentinel, Baycor 25
cyprodanil (anilinopyrimidine)Unix
cyprofuram ( -[N-(3-chlorophenyl)cyclopropanecarboxamido]- butyrolactone)Vinicur, SN 78314, Stanza
diazoben (sodium p-(dimethylamino)-benzene diazosulfonate)Dexon
dichlobutrazol ((2RS,3RS)-1-(2,4-dichlorophenyl)-4,4-dimethyl-2-(1H1,2,4-triazol-1-yl) pentan-3-ol)Vigil
dichlofluanid (N-dichlorofluoromethylthio-N',N'-dimethyl-Nphenylsulphamide)Elvaron, Euparen, Euparene, Bay 47531
dichlone (2,3-dichloro-1,4-naphthoquinone)Quintar, Phygon
dichlorophen (4,4'-dichloro-2,2'-methylenediphenol)Super Mosstox,
Mosstox, Bio Moss Killer, Panacide
diclomezine (6-(3,5-dichloro-4-methyl-phenyl)-3(2H)pyridazinone)
Monguard
dicloran (2,6-dichloro-4-nitroaniline)Botran, Allisan, Resisan, Kiwi
Lustr, Marisan, Scleran, Sclerosan, DCNA, Ditranil
difulmetorim (RS-5-chloro-N[1-(4-difluoromethoxy phenyl)propyl]-6methyl-primidin-4-ylamine)Pyricut, UBF002EC
dimethirimol (5-butyl-2-dimethylamino-6-methylpyrimidin-4-ol)
Milcurb
dimethomorph ((E,Z)-4-[3-(4-chlorophenyl)-3-(3,4dimethoxyphenyl)acrylolyl] morpholine)Acrobat, Forum
diniconazole ((E)-(RS)-1-(2,4-dichlorophenyl)-4,4-dimethyl-2-(1H-1,2,4triazol-1-yl)pent-1-en-3-ol)Sumi8, Sumieight, Spotless, S3308L,

Ortho Spotless, XE779L
dinobuton (2-sec-butyl-4,6-dinitrophenyl isopropyl carbonate)Acrex,
Dessin, Drawinol, Dinofen, Talan
dinocap (2 (or 4)-(1-methylheptyl)-4,6(or 2,6)-dinitrophenyl crotonate)
Karathane, Ezenosan, Crotothane, Caprane, Cekucap, Fenocap
ditalimfos (O,O-diethyl phthalimidophosphonothioate)Plondrel,
Laptran, Millie, Farmil, Dowco 199, Frutogard, Leucon
dithianon (5,10-dihydro-5,10-dioxonaphtho(2,3-b)-1,4-dithiin-2,3dicarbonitrile)Delan, DelanCol
DNOC (4,6-dinitro-o-cresol)ExtarA, ExtarLin, Sandoline, Sinox,
Selinon, Trifocide, Trifina, Cresofin, Chemsect, Antinonnin, Elgetol,
Nitrador
dodemorph acetate (4-cyclododecyl-2,6-dimethylmorpholinium
acetate)Meltatox, BASF-Mehltaumittel, Milban
dodine (1-dodecylguanidinium acetate)Cyprex, Melprex, Venturol,
Carpene, Syllit, Curitan, Vondodine, Efuzin
drazoxolon (4-(2-chlorophenyl hydrazone)-3-methyl-5-isoxazolone)
Ganocide, MilCol, SAIsan, PP 781
edifenphos (O-ethyl S,S-diphenyl phosphorodithioate)Hinosan, Bay
78418
etaconazole (1-[2-(2,4-dichlorophenyl)-4-ethyl-1,3-dioxolan-2-ylmethyl]1H-1,2,4-triazole)Benit, Sonax, Vangard, CGA 64251
ethirimol (5-butyl-2-ethylamino-6-methylpyrimidin-ol)Milgo,
Milstem, Milcurb Super, PP149
etridiazole (5-ethoxy-3-trichloromethyl-1,2,4-thiadiazole)AAterra,
Dwell, Koban, Pansoil, Terrazole, Truban
famoxadone (3-anilino-5-methyl-5-(4-phenoxyphenyl)-1,3-oxazolidine2,4-dione)Famoxate, DPX-JE874
fenaminosulf (sodium 4-dimethylaminobenzenediazosulphonate)Lesan,
Bay 5072, Bay 22555
fenarimol (2,4'-dichloro- -(pyrimidin-5-yl)benzhydyl alcohol)Bloc,
EL222, Rubigan, Rimidin, Fenal
fenfuram (2-methyl-3-furanilide)Panoram, Pano-ram
fenhexamid (N-(2,3-dichloro-4-hydroxyphenyl)-1-methylcyclohexanecarboxamide)KBR 2738
fenpropidin ((RS)-1-[3-(4-tert-butylphenyl)-2-methylpropyl]piperidine)
Patrol, Ro 123049, Tern
fenpropimorph (cis-4-[3-(4-tert-butylphenyl)-2-methylpropyl]-2,6dimethylmorpholine)Corbel, Mistral
fentin acetate (triphenyltin acetate)Brestan, Chimate, Batasan, Fenilene,

Fentol, Hoe 2824, LiroTin, Pandar, PhenostanA, Stanex, Suzu,
Tinestan, Ucetin
fentin chloride (triphenyltin chloride)Aquatin, Phenostat C, Tinmate
fentin hydroxide (triphenyltin hydroxide)Brestan Flow, DuTer, Duter,
Flo Tin, Haitin, SuzuH, Tubotin, Farmatin, SuperTin, Phenostat H,
Triple Tin
ferbam (ferric dimethyldithiocarbamate)AAfertis, Carbamate, Ferbam,
Knockmate, Trifungol, Ferberk, Hexaferb
fluazinam (3-chloro-N-(3-chloro-5-trifluoromethyl-2-pyridyl)- , , ,trifluoro-2,6-dinitro-p-toluidine)Shirlam Flow, Shirlan
fluoromide (2,3-dichloro-N-4-fluorophenylmaleimide)Spartcide,
MK-23
fluotrimazole (1-(3-trifluoromethyltrityl)-1H-1,2,4-triazole)Bay BUE
0620, Persulon
flusilazol (1-[bis(4-fluorophenyl)methylsilylmethyl]-1H-1,2,4triazole)Punch, Nustar, Olymp, DPX H6573
flutolanil ( , , -trifluoro-3'-isopropoxy-o-toluanilide)Moncut, NNF136, Prostar 4E, Monstar
flutriafol ((RS)-2,4'-difluoro--(1H-1,2,4-triazol-1-ylmethyl)benzhydryl
alcohol)Impact, PP 450, Vincit
folpet (N-(trichloromethylthio) phthalimide)Phaltan, Fungitrol, Folpan
fosetyl-aluminium (aluminium tris ethyl phosphonate)Aliette, LS
74783
fthalide (4,5,6,7-tetrachlorophthalide)Rabcide, KF-32
fuberidazole (2-(2'-furyl)benzimidazole)Voronit, Bay 33172,
Fuberidazole
furalaxyl (methyl N-(2-furoyl)-N-(2,6-xylyl)-DL-alaninate)Fonganil,
Fongarid, CGA38140
furmecyclox (methyl N-cyclohexyl-2,5-dimethylfuran-3carbohydroxamate)Campogran, Xyligen B, BAS 389F, Epic
guazatine (acetates of a mixture of the reaction products from polyamines
[comprising mainly octamethylenediamine, iminodi(octamethylene)
diamine, and octamethylenebis (iminooctamethylene)diamine] and
carbamonitrile)Panoctine, Panolil, Radam, Kenopel, Rappor
hexachlorobenzene (hexachlorobenzene)Anticarie, No Bunt, Ceku C.B.
hexaconazole (RS-2[2,4-dichlorophenyl]-1-[1H-1,2,4-triazol-1-yl] hexan2-ol)Anvil, PP523, Planete Aster
hydroxyquinoline sulphate (bis(8-hydroxyquinolinium)sulfate)
Chinosol, Albisal, Cryptonol
imazalil (1-( -allyloxy-2,4-dichlorophenylethyl)imidazole)Bromazil,
Deccozail, Fecundal, Fungaflor, Fungazil, Freshgard
imibenconazole (4-chlorobenzyl-N-(2,4-dichlorophenyl)-2-(1H-1,2,4triazol-1-yl) thioacetimidate)Manage

iminoctadine tris (1,1'-iminiodi-(octamethylene)diguanidinium)
Bellkute
iprobenfos (S-benzyl O,O-di-isopropyl phosphorothioate)Kitazin
iprodione (3-(3,5-dichlorophenyl)-N-isopropyl-2,4-dioxoimidazolidine-1carboxamide)Rovral Flo, Rovral WP, 26019 RP, Chipco 26019, LFA
2043, Kidan, Verisan
iprovalicarb (strobilurin compound)SZX 0722
isoprothiolane (di-isopropyl 1,3-dithiolan-2-ylidenemalonate)Fuji-one,
Isoran, Fudiolan, NNF109, SS 11946
kasugamycin (5-amino-2-methyl-6(2,3,4,5,6-pentahydroxycyclohexyloxy)tetrahydropyran-3-yl)amino- -iminoacetic acid)Kasumin
kresoxim methyl (methyl methoxyiminoacetate)Stroby WG, Candit,
BASF 490F
lime sulfur (calcium polysulfide)Orthorix, Security Lime Sulfur
mancozeb (manganese-zinc ethylenebis dithiocarbamate)Dithane 945,
Penncozeb, Dithane M45, Manzate 200, Nemispor, Vondozeb Plus,
Sandozebe, Mancozin, Manzin, Policar MZ, Manzeb, Azko Chemie
Mancozeb, Policar S, Ziram-Dithane
maneb (manganese ethylenebis dithiocarbamate)Dithane M22, Maneb,
Lonacol M, Tersan, Trimangol, Nespor, Manesan, Farmaneb, Plantineb
Roussel Uclaf, Polyram M, Mangavis, Manebgan, Remasan,
Rhodianebe, Kypman 80, Akzo Chemie Maneb, Maneb Spritzpulver,
Manex 80, Maneba, Manex, Manzate, Manzate D, MDiphar, Chloroble
M, Sopranebe, Tubothane, Unicrop Maneb
mepronil (3'-isopropoxy-o-toluanilide)Basitac
mercuric oxide (mercury oxide)Santar, Kankerdood
mercurous chloride (mercury chloride)Cyclosan
metalaxyl (methyl N-(2-methoxyacetyl)-N-(2,6-xylyl)-DL-alaninate)
Apron, Ridomil, Subdue, CGA 48988
metconazole ((1RS, 5RS; 1RS, 5SR)-5-(4-chlorobenzyl)-2,2-dimethyl-1(1H-1,2,4-triazol-1-ylmethyl)cyclopentanol)Caramba
metham-sodium (sodium methyldithiocarbamate)Vapam, VPM,
Arapam, Busan, Solasan, Trimaton, Maposol, Sistan, Sometam,
Monam, Nemasol
methasulfocarb (S-(4-methylsulfonyloxy-phenyl)-Nmethylthiocarbamate)Kayabest
methoxyethylmercury acetate (2-methoxyethylmercury acetate)
Panogen-Metox, Panogen M, Cekusil Universal A
methoxyethylmercury chloride (2-methoxyethylmercury chloride)
Emisan 6 percent
methyl bromide (bromomethane)Meth-O-Gas, Terr-O-Gas 100, Haltox

methyl isothiocyanate (methyl isothiocyanate)Trapex
metiram (zinc ammoniate ethylenebis(dithocarbamate)polyethylenethiuram disulfide)Polyram, PolyramCombi, Carbatene
metomeclan (1-(3,5-dichlorophenyl)-3-methoxy-methyl-2,5pyrrolidindione)Drawifol
metominostrobin ((E)-2-methoxyimino-N-methyl-2-(2phenoxyphenyl)acetamide)Oribright
myclobutanil ( -butyl- -(chlorophenyl)-1H-1,2,4-triazole-1propanenitrile)Systhane, RH3866, Eagle, Nova, Rally
nabam (disodium ethylenebis (dithiocarbamate))Dithane A-40,
Nabasan, Chem Bam, DSE, Parzate, Spring Bak
nitrothal-isopropyl (di-isopropyl 5-nitroisophthalate)used as mixed formulations with other fungicides
nuarimol (2-chloro-4'-fluoro- -(pyrimidin-5-yl) benzhydryl alcohol)
Triminol, Trimidal, EL228, Gauntlet, Murox
octhilinone (2-octylisothiazol-3(2H)-one)Pancil-T, RH-893
ofurace (2-chloro-N-(2,6-dimethylphenyl)-N-(tetrahydro-2-oxo-3furanyl)acetamide)Ortho 206 15
oxadixyl (2-methoxy-N-(2-oxo-1,3-oxazolidin-3-yl)acet-2',6'-xylidide)
Sandofan, SAN 371F, Recoil, Ripost, Wakil, Pulsan
oxine copper (cupric 8-quinolinoxide)Quinolate, Cunilate, Dokirin,
Fruitdo, Quinondo, Cellu-Quin, Bioquin, Milmer
oxycarboxin (5,6-dihydro-2-methyl-N-phenyl-1,4-oxathiin-3carboxamide-4,4-dioxide)Plantvax, F461, Oxykisvax, Carbexin
pefurazoate (pent-e-enyl N-furfuryl-N-imidazol-1-ylcarbonyl-DLhomoalaninate)Healthied
penconazole (1-(2,4-dichloro- propylphenethyl)-1H-1,2,4-triazole)
Topas, Topaz, Topazo, Onmex, Award, CGA 71818
pencycuron (1-(4-chlorobenzyl)-1-cyclopentyl-3-phenylurea)
Monceren, Bay NTN 19701
pentachlorophenol (pentachlorophenol)Penchloral, Pentacon, Penwar,
Sinituho
phenylmercury acetate (phenylmercury acetate)Ceresol, Agrosan,
Cekusil, Celmer, Pamisan, Seedtox, PMAS, Unisan, Hong Nien, Phix,
Mersolite
phenylphenol (biphenyl-2-ol)Dowicide 1, Nectryl
phosdiphen (bis(2,4-dichlorophenyl)ethyl phosphate)MTO460
pimaricin ((8E, 14E, 16E, 18E, 20E)-(1S, 3R, 5S, 7S, 12R, 24R, 25S,
26R)-22-(3-amino-3,6-dideoxy- -D-mannopyranosyloxy)-1,3,26-
trihydroxy-12-methyl-10-oxo-6,11,28-trioxatricyclo
(22.3.1.05,7)octacosa-8,14,16,18,20-pentaene-25-carboxylic acid)]
Delvolan
polyoxins [5-(2-amino-5-O-(aminocarbonyl)-2-deoxy-L-xylonyl)amino]1,5-dideoxy-1-[3,4-dihydro-5-(hydroxymethyl)-2,4-dioxo-1(2H)pyrimidinyl]- -D-allofuranuronic acid (polyoxin B); 1-5-[[2-amino-5O-(aminocarbonyl)-2-deoxy-L-xylonyl]amino]-5-deoxy- -Dallofuranuronosyl]-1,2,3,4-tetrahydro-2,4-dioxo-5-pyrimidinacid
(polyoxin D)Polyoxin AL-polyoxin B, Piomycin-polyoxin B,
Polyoxin Z-polyoxin D zinc salt, Piomy, Pio
probenazole (3-allyloxy-1,2- benzoisothiazole 1,1-dioxide)Oryzemate,
Probenazol
prochloraz (1-N-propyl-N-[2-(2,4,6trichlorophenoxy)ethyl]carbamoylimidazole)Sportak, Sporgon
procymidone (N-(3,5-dichlorophenyl)-1,2-dimethylcyclopropane-1,2dicarboximide)Sumilex, Sumisclex, S-7131
propamocarb hydrochloride (propyl 3-(dimethylamino)propylcarbamate
hydrochloride)Filex, Previcur N, SN 66752, Prevex, Banol
propiconazole (1-[2-(2,4-dichlorophenyl)-4-propyl-1,3-dioxolan-2ylmethyl]-1H-1,2,4-triazole)Radar, Tilt, Desmel, Banner, CGA
64250, Bumper, Orbit, Alamo
propineb (polymeric zinc propylenebis(dithiocarbamate))Antracol, Bay
46131, LH 30/Z, Airone, Taifen
propionamide (N-(1-cyano-1,2-dimethyl propyl)-2-(2,4-dichlorophenoxy)
propionamideAC382042
pyrazophos (O,O-diethyl-O- (5-methyl-6-ethoxy-carbonyl-pyrazolol
(1,5a)-pyrimid-2-yl)thionophosphate)Afugan, Curamil, Missile, Hoe
02873, Pokon Mildew Spray
pyrifenox (2',4'-dichloro-2-(3-pyridyl)acetophenone O-methyloxime)
Dorado, ACR3651 A, Ro 151297
pyroquilon (1,2,5,6-tetrahydropyrrolo(3,2,1-ij)quinolin-4-one)
Fongoren, Fongorene, CGA 49104
quinoxyfen (4-phenoxyquinoline)DE795
quintozene (pentachloronitrobenzene)Brassicol, Tubergran, Terraclor,
Folosan, Tritisan, Saniclor, Avicol, Kobutol, Pentagen, Botrilex, Kobu,
Earthcide, Turfcide, RTU-PCNB, PCNB, Quintozene, Tilcarex,
TriPCNB
spiroxamine (spiroketal)Impulse, KWG 4168
sulfur (sulfur)Kumulus, Solfa, Thiovit, Cosan, Thiolux, Thion, Sulfex,
Elosal, Suffa, Super Six, Sulfur Alfa, Microthiol Special
tecnazene (1,2,4,5-tetrachloro-3-nitrobenzene)Fusarex, Bygran, Hytec,

Nebulin, Hickstor, Hystore, Arena, Easytec
thiabendazole (2-(thiazol-4-yl)benzimidazole)Tecto, Mertect, Arbotect,
Thibenzole, Comfuval, Apl-Luster, Storite, Mycozol, TBZ
thiophanate (diethyl 4,4'-(o-phenylene)bis(3-thioallophanate))Topsin E
thiophanate-methyl (dimethyl 4,4'-(o-phenylene)bis(3-thioallophanate))
Cecrobin, Topsin M, Seal&Heal, Mildothane, Enovit, Frumidor,
Sipcaplant, Pelt 44, Thiophan, NF44
thiram (tetramethylthiuram disulfide)Hexathir, Thiotox, Tripomol,
Fernide, Vancide TM, Polyram Ultra, Cunitex, HyVic, Tetrapom,
Spotrete, Thiramad, TMTD, AAtack, Aules, Chipco Thiram, Fermide
850, Fernasan, Mercuram, Nomersam, Pomarsol forte, SpotreteF,
Tetrapom, Thimer, Thioknock, Thiotex, Thirasan, Thiuramin, Tirampa,
TMTDS, Trametan, Tripomol, Tuads
tolclofos-methyl (O-2,6-dichloro-p-tolyl O,O-dimethyl
phosphorothioate)Rizolex, S3349
tolylfluanid (N-dichlorofluoromethylthio-N',N'-dimethyl-N-ptolysulphamide)Euparen M, Bay 49854
triadimefon (1-(4-chlorophenoxy)-3,3-dimethyl-1(1H-1,2,4-triazol-1yl)butanone)Bayleton, Amiral, Bay MEB 6447, Acizol, Strike
triadimenol (1-(4-chlorophenoxy)-3,3-dimethyl-1(1H-1,2,4-triazolyl)butan-2-ol)Bayfidan, Baytan, Summit
trichlamide ((RS)-N(1-butoxy-2,2,2-trichloethyl)salicylamide)
Hataclean
tricyclazole (5-methyl-1,2,4-triazolol(3,4-b)benzothiozole)Beam, Bim
tridemorph (reaction products of C11-C14 4-alkyl-2,6-dimethylmorpholine
homologues containing 60 to 70 percent of 4-tridecyl isomers)
Bardew, Calixin, Ringer
triflumizole ((E)-4-chloro- , , -trifluoro-N-(1-imidazol-1-yl-2propoxyethylidene)-o-toluidine)Trifmine, NF114
triforine (1,1'-piperazine-1,4-diyldi-[N-(2,2,2trichloroethyl)formamide])Saprol, Cela W524, Funginex, Denarin
validamycin A ([1S-(1 , 4 , 5 , 6 )]-(1,5,6-trideoxy-3-O- -Dglucopyranosyl-5-(hydroxymethyl)-1-[[4,5,6-trihydroxy-3(hydroxymethyl)-2-cyclohexen-1-yl]amino]-D-chiro-inositol])
Validacin, Valimon
vinclozolin (3-(3,5-dichlorophenyl)-5-ethenyl-5-methyl-2,4oxazolidinedione)Ronilan FL, Curalan, Ornalin, Vorlan, Vinclosin
zineb (zinc ethylene bisdithiocarbamate)Dithane Z 78, Lonacol,
Zinosan, Ditozin, PolyramZ, Hexathane, Zineb, Hortag, Tritoftorol
ziram (zinc dimethyldithiocarbamate)Cuman, Corozate, Mezene,

Pomarsol Z, Carbazinc, Fungostop, Zirex, Ziram, Drupina 90, Hexazir,
Mezene, Prodaram, Tricarbamix Z, Triscabol, Zerlate, Vancide MZ96,
Zincmate, Ziram Technical, Ziram F4, Ziram W76, Ziramvis, Zirasan
90, Zirberk, Ziride, Zitox
Ready-Formulated Mixtures of Fungicides
Many chemical companies produce a mixture of fungicides, mixing different active ingredients (Research Information Ltd., 1998). These readyformulated mixtures increase the potential spectrum of activity. The mixtures may be of site-specific chemicals with different modes of action, or
involve multisite components. This mixture will reduce the risk of the development of fungicide-resistant strains, as the pathogen must mutate to
overcome two modes of action simultaneously. The important commercially available mixed formulations of fungicides are listed as follows.
Some mixtures contain insecticides (lindane, gamma-HCH, diazinon, furathiocarb) and/or micronutrients (molybdenum, manganese, zinc, iron, copper) in addition to fungicides. The following list provides the names of the
active ingredients in the mixtures and their trade names (in parentheses).
benalaxyl+mancozeb (Galben M)
benalaxyl+mancozeb+fosetyl (Carlit)
benomyl+mancozeb (Manzate)
benomyl+thiram (Benlate T)
bitertanol+fuberidazole (Sibutol)
bromophos+captan+thiabendazole (Bromotex T)
bromuconazole+iprodione (But)
bupirimate+chlorothalonil (Dovetail)
bupirimate+hexaconazole (Oscar)
bupirimate+triforine (NimrodT)
captan/pyrifenox+dithianon or mancozeb (Rondo)
captan+DCNA (Captan DCNA 60200)
captan+dichloran (Botec)
captan+fosetyl aluminium+thiabendazole (Aliette Extra)
captan+quintozene+thiabendazole (Rival)
carbendazim+chlorothalonil (Bravocarb, Greenshield)
carbendazim+chlorothalonil+maneb (Victor)
carbendazim+fenpropimorph (Corbel-Duo, Corbel TX)
carbendazim+flutriafol (Early Impact)
carbendazim+flutriafol+chlorothalonil+pyrazophos (Impact)
carbendazim+hexaconazole (Planete R)
carbendazim+iprodione (Vitesse)
carbendazim+mancozeb (Kombat WDG)
carbendazim+mancozeb+sulfur (Kombat S)
carbendazim+maneb (MultiW FL, Delsene M Flowable, Delsene
MX Septal, Bavistin M, Bavistin M72, Headland Dual, Legion)
carbendazim+maneb+sulfur (Bolda FL)
carbendazim+maneb+tridemorph (Cosmic FL)
carbendazim+prochloraz (Sportak Alpha)
carbendazim+propiconazole (Hispor 45 WP)
carbendazim+tecnazene (Hortag Tecnacarb Dust, Arena Plus)
carbendazim+thiram+gamma HCH (Gammalex Liquid)
carbendazim+vinclozolin (Konker)
carboxin+captan (Enhance)
carboxin+diazinon+lindane (Germate Plus)
carboxin+imazalil+thiabendazole (Cerevax Extra, Vitavax Extra)
carboxin+maneb+lindane (Enhance Plus)
carboxin+metalaxyl+quintozene (Prevail)
carboxin+PCNB (VitavaxPCNB)
carboxin+phenylmercury acetate (Murganic RPB)
carboxin+thiabendazole (Cerevax)
carboxin+thiram (Vitavax200, Vitavax 75W, RTUVitavax Thiram,
Vitavax Flo)
carboxin+thiram+gamma HCH (Vitavax RS Flowable)
carboxin+thiram+lindane (Gustafson Vitavax-Thiram-Lindane)
chlorothalonil+carbendazim (Bravocarb)
chlorothalonil+copper oxychloride (Clortocaffaro Ramato)
chlorothalonil+fenpropimorph (Corbel CL, Corbel-Star, CorbelFort)
chlorothalonil+flutriafol (Impact Excel, Halo)
chlorothalonil+metalaxyl (Folio 575 FW, Ridomil plus Bravo W,
Ridomil CT)
chlorothalonil+propamocarb (Tattoo C)
copper oxychloride+copper carbonate+copper sulfate+mancozeb
(Tri-Miltox)
copper oxychloride+copper sulfate+copper carbonate+folpet
(Trimifol)
copper oxychloride+maneb+sulfur (Ashlade SMC)
copper oxychloride+sulfur (Wacker 83 and Wacker 83v)
copper oxychloride+zineb (Miltox)
copper sulfate+cufraneb (Comac Macuprax)
copper sulfate+sulfur (Stoller, TopCop)
cymoxanil+mancozeb (Fytospore, Ripost M)

cymoxanil+propineb (Diametan, Milraz)
dimethomorph+mancozeb (Acrobat WG)
epoxiconazole+fenpropimorph (Opus Team, Opus Top)
epoxiconazole+thiophanate-methyl (Rex)
epoxiconazole+tridemorph (Tango, Opus Forte)
ethirimol+flutriafol+thiabendazole (Ferrax)
fenarimol+dodine (Rubigan Plus)
fenpropimorph+chlorothalonil+carbendazim (Corbel Triple)
fenpropimorph+fenpropidin (Boscor)
fenpropimorph+fenpropidin+prochloraz (Towrnoi)
fenpropimorph+iprodione (Sirocco)
fenpropimorph+prochloraz (Sprint, Magic)
fenpropimorph+thiram+gamma HCH (LindexPlus FS)
fenpropimorph+thiram+lindane (Lindex-Plus FS Seed Treatment)
fenpropimorph+tridemorph (Gemini, Rockett Flowable, Rockett
Ultra)
fentin acetate+maneb (Brestan 60, Trimastan)
fentin hydroxide+maneb+zinc (Chiltern Tinman)
fentin hydroxide+metoxuron (Endspray)
fentin hydroxide+sulfur (Pennsuc S)
ferbam+maneb+zineb (Trimanzone, Tricarbamix)
flusilazole+chlorothalonil (Triumph)
flusilazole+tridemorph (Meld, Sherif/Gal)
fosetyl+captan+carbendazim (Aliette III WG)
fosetyl+folpet (Mikal)
fosetyl+folpet+cymoxanil (Valiant)
fosetyl+mancozeb+cymoxanil (R6 Triplo)
fuberidazole+imazalil+triadimenol (Baytan IM)
guazatine+imazalil (Rappor Plus)
hexaconazole+fenpropidin (Jupiter)
iodophor+tecnazene (Bygran F)
iodophor+thiabendazole (Byatran, Tubazole)
iprodione+thiophanate-methyl (Compass)
kresoxim methyl+epoxiconazole (Juwel 250, Allegro)
kresoxim methyl+mancozeb (Kenbyomix)
mancozeb+benalaxyl (Galben M)
mancozeb+cymoxanil (Fytospore)
mancozeb+fosetyl (Rhodax)
mancozeb+metalaxyl (Fubol 58 WP, Ridomil MZ)
mancozeb+ofurace (Patafol)
mancozeb+oxadixyl (Recoil, Sandofen M 8)
mancozeb+pyrifenox (Furado)
maneb+zinc oxide (Mazin)
maneb+zineb (Luxan Zinnanaat, Triziman, Vondozeb)
metalaxyl+copper (Ridomil plus)
metalaxyl+copper+folpet (Acylon)
metalaxyl+folpet (Ridomil combi)
metalaxyl+metiram (Arcerid)
metalaxyl+PCNB (ApronTerraclor, Ridomil PC)
metalaxyl+thiabendazole+furathiocarb (Rapcol TZ)
metalaxyl+thiabendazole+thiram (Apron Combi 453 FS)
methyl bromide+chloropicrin (Methyl Bromide-Chloropicrin
Mixtures)
methylisothiocyanate+dichloropropene (Vorlex)
metiram+cymoxanil (Aviso DF, Aviso G, Aviso C, Aviso S, Aviso)
metiram+cymoxanil+copper oxychloride (Aviso Cup)
metiram+metiram zinc (Polycarbacin)
metiram+nitrothal-isopropyl (Pallinal, Pallitop)
metiram+ofurace (Aviso Combi)
nuarimol+chlorothalonil (Guardaton)
nuarimol+imazalil (Elanco Seed Treatment)
nuarimol+mancozeb (Tridal M)
oxadixyl+cymoxanil+dithiocarbamate+phtalimide (Ripost, Pulsan)
oxadixyl+propineb (Vitiril, Fruvit)
oxine-copper+copper hydroxide (Kinset WP)
penconazole+captan (Topas C)
penconazole+mancozeb (Topas MZ)
phenylmercury acetate+gamma HCH (Mergamma 30)
prochloraz+carbendazim (Sportak Alpha, Sportak Alpha/PF)
prochloraz+carbendazim+tebuconazole (Troika)
prochloraz+carboxin (Abavit)
prochloraz+cyproconazole (Sportak Delta 460, Sportak
Delta/Tiptor, Tiptor)
prochloraz+fenpropidin (Sponsor)
prochloraz+fenpropimorph (Sprint, Sprint HF)
prochloraz+manganese chloride complex (Octave)
propamocarb+mancozeb (Tatoo)
propiconazole+carbendazim (Tilt CB)
propiconazole+chlorothalonil (Tilt CT)
propiconazole+fenpropidin (Zenit)
propiconazole+fenpropimorph (Archer,Tilt Top)
propiconazole+imazalil+thiabendazole (Benit Universal)
propiconazole+tridemorph (Tilt Turbo)
propineb+oxadixyl (Fruvit)
sulfur+nitrothal-isopropyl (Kumulan)
tebuconazole+imazalil (Premis Delta)
tecnazene+organo iodine (Bygran F)
tecnazene+thiabendazole (Hytec Super, Storite SS)
thiabendazole+imazalil (Extratect Flowable)
thiabendazole+thiram (HYTL, Ascot 480 FS, HyVic, RTU Flowable
Soybean Fungicide)
thiabendazole+thiram+gamma HCH (New Hysede FL)
thiram+gamma-HCH (Hydraquard, Hysede FL)
triadimenol+thiram (RTU-Baytan-Thiram)
triadimenol+tridemorph (Dorin)
triticonazole+anthraquinone (Real)
triticonazole+iprodione (Raxil Complex)
vinclozolin+chlorothalonil (Ronilan Spezial)
vinclozolin+maneb (Ronilan M)
vinclozolin+thiram (Ronilan T-Combi, Silbos DF)
INGREDIENTS IN FUNGICIDE FORMULATIONS
Laboratory-tested active ingredients alone are not normally useful for
field application and are rarely applied in their pure form. These active ingredients are formulated to make them suitable for large-scale field use.
Formulations are vehicles that enable the active ingredient to be applied under a variety of conditions without loss in performance. These formulations
should be safe to the crop, easy to handle, and compatible with other major
products. The ideal formulation should deliver the fungicide (active ingredient) in a manner that maintains its intrinsic activity or enhances its performance through enhanced redistribution or mobility. The formulation should
reduce losses through volatile action and increase the persistence of the
product for a long time. Formulation strategies may need to be designed for
each type of active ingredient. If the selected active material acts by redistribution in the crop through the vapor phase, the formulation should not inhibit volatile action of the active ingredient. Similarly, if the active ingredient acts as a surface-acting protectant, the formulation, which prevents
wash-off in rain by increasing penetration of the fungicide into the plant,
will lose its activity.
Active ingredients are normally mixed with inert (inactive) ingredients
so that the mixture can be handled more conveniently and safely and can be
applied more easily and efficiently. The formulations also consist of several
adjuvants.
Adjuvants are materials added to improve some chemical or physical property of a plant protectant. Adjuvants increase efficiency of the products and
reduce drift. The adjuvants used in the fungicide formulations are wetting
agents, dispersing agents, spreaders, stickers, UV filters, surfactants, emulsifiers, foam suppressors, penetrants, and drift control agents.
Emulsifiers are chemicals that assist the formation of suspension of small
droplets (often of colloidal dimensions) of one liquid in another in which the
former is insoluble. They help in the formation of an emulsion comprising
small spheres of organic solvent/fungicide in the sprayer.
Foam suppressors are surface-active substances that form a fast-draining
foam to provide maximum contact of the spray to the plant surface.
Penetrants are wetting agents, oils, or oil concentrates that enhance the absorption of a systemic fungicide by the plant. Agri-Dex, Induce, and
Penetrator are the commercially available penetrants.
Stickers facilitate persistence of the applied fungicide on foliage even during rain.
Surfactants are surface-active agents capable of dissolving in both water
and organic solvents, and therefore, they can help in mixing water-insoluble
fungicides in water. Surfactants reduce surface tension through adsorption
at air-water or water-solid interfaces. The active ingredients are generally
insoluble in water but soluble in lipophilic, organic solvents such as cyclohexane and xylene. The solvents are used in fungicide formulations. The
fungicide dissolved in organic solvents would not be miscible with water in
the spray tank and therefore would not be delivered during the application
process. Surfactants help in forming suspension of fungicides in water. Surfactants have several functions. These include retention of the spray, wetting
of the leaf surface, and enhancement of uptake of the active ingredient. Surfactants in fungicide formulations increase penetration of the fungicide into
the plant subsurface. Surfactant sorption by plant cuticles has been demonstrated. Water-soluble surfactants increase spray droplet size compared to
water alone (Ellis and Tuck, 2000). Polyethoxy derivatives (Triton X surfactants) and Tween 85, Tween 21, Tween 80, Tween 40, and Tween 20 are the
important surfactants.
UV filters increase photolytic stability of the fungicide on foliage.
Wetting agents and dispersing agents are added to assist in particle suspension during application.
The manufactured fungicide formulations themselves often contain all

the necessary adjuvants in appropriate amounts. However, addition of some
adjuvants to the spray fluid may increase efficacy of the fungicide severalfold (Kwon et al., 1998). Several adjuvants are screened for their effect in
increasing efficacy of various active ingredients. For example, alcohol
ethoxylate surfactants increase the efficacy of dimethomorph (Grayson et al.,
1996) and prochloraz (Stock, 1996). Addition of ethylene oxide-propylene
oxide block copolymers and ethylene oxide-propylene oxide-propylene oxide ethylenediamines to the spray fluid forms larger spray deposits with
better coverage on foliage (Richards et al., 2000). Some adjuvants such as
Sandovit and SBR 0110 act as rainfasteners and improve rainfastness of
fungicides.
Several proprietary wetting agents, surfactants, emulsifiers, spreading
agents, and stickers are available in the market. They can be mixed in the
spray tank to increase the efficacy of the fungicides. The commercially
available adjuvants are listed in the following section.
Available Adjuvants
(Names of the suppliers of the adjuvants are given in parentheses)
Acerpenetrating acidifying surfactant (Tripart Farm Chemicals)
Activator 90a low-foaming, spreader adjuvant that provides quick
wetting, more uniform distribution, and increased retention of
spray by reducing surface tension of the spray droplet (Loveland)
Add-It-To-Oila natural oil emulsifier (Drexel)
Agralnonionic wetting and spreading agent (Zeneca)
Agrol Plus Sadjuvant to increase efficiency of benomyl,
mancozeb, and zineb (Chemol)
Agurawetting agent giving improved adhesion of spray fluid to
crops (Agro-Kanesho)
Bionexa powerful spreading, wetting, and penetrating agent that
reduces the surface tension in sprays; it permits a uniform film to
be formed on sprayed surfaces, ensuring the total coverage of
treated foliage (Grupo Bioquimico Mexicano)
Bonda sticker that acts as a deposition agent by binding fungicide
sprays to the leaf surface; it prevents loss of fungicidal activity
due to heat, wind, rain, and sprinkler irrigation (Loveland)
Carriera vegetable oil surfactant (Stoller)
Citowettnonionic spreader-sticker and wetting agent (BASF)
Croptex Z.I.P.a spreader and buffering agent; functions as wetting

agent; lowers pH of spray solutions thereby improving effectiveness of many sprayed products (Hortichem)
Deposita drift-retardant additive that hydrates fungicides
(Loveland)
Eyearfoamless wetting agent which enhances and improves
emulsifiability and suspensibility of spray preparations (AgroKanesho)
Haitenspreader-sticker of high efficacy (Hokko)
Headland Guardsticking and spreading agent (Headland)
Headland Intakewetting and penetrating agent (Headland)
KK StickerSticking and wetting agent which provides enhanced
retention of fungicides on plant surfaces and reduces weathering
(Agro-Kanesho)
Lentus Aa synthetic latex surfactant, useful as a sticker and extender (spreader) improving rainfastness (Tripart Farm Chemicals)
Li 700penetrating surfactant, improves penetration of the fungicide into plant tissue (Loveland)
Lo-Driftspray additive to reduce drift (Rhone-Poulenc)
Minaxnonionic wetter for use with fungicides to improve spreading penetration (Tripart Farm Chemicals)
Neptunea spreader-wetter (Loveland)
Nonitwetting agent to increase the wettability of fungicide sprays
(Chemol)
PBI Spreadernonionic spreading and wetting agent (Pan Britannica)
Petan V Stickerwetting agent with high adherence and persistence
and reduced weathering (Agro-Kanesho)
Pinene IIa nonionic spreader sticker adjuvant (Drexel)
Plyaca spreader-sticker which dries quickly to produce a waterresistant, elastic film, which increases spray deposits and reduces
weathering (Loveland)
Sandovitsurface-active adjuvant (Sandoz Agro)
Sil-Factsilicone surfactant with fast wetting properties and
stomatal flooding (Drexel)
Spreadernonionic spreading and wetting agent (Pan Britannica)
SP Superwetting agent and sticker (Productos OSA)
Surf-AC 820nonionic surfactant (Drexel)
Surf-AC 910nonionic surfactant (Drexel)
Wettolemulsifier and wetting and dispersing agent for formulating

fungicides (BASF)
Wetza nonionic surfactant in an antifoam system for use with fungicides (Drexel)
TYPES OF FUNGICIDE FORMULATIONS
Fungicides are formulated in several ways, depending on their physical
characteristics and application methods. Fungicides used for seed treatment
should be formulated differently from those used as foliar spray. Fungicides
that are soluble in water should be formulated differently from those that are
insoluble in water. Fungicides are formulated as wettable powders, dustable
powders, driftless dusts, emulsifiable concentrates, water-soluble powders,
soluble concentrates, suspension concentrates, granules, flowable formulations, water-soluble bags, oil-miscible liquids, oil-dispersible powders, water-miscible concentrates, liquid formulations, aqueous suspensions, ULV
formulations, oil formulations, coating agents, fumigants, dry seed treatment, liquid seed treatment, flowable seed treatment, and soil treatment formulations. These formulations consist of various types of adjuvants.
Wettable Powders
Wettable powders are solid formulations suitable for fungicides that have
low solubility in water. They are produced by crushing the solid active ingredient and an inorganic diluent, such as clay, talc, or silica, in a ball mill to
a particle size of about 25 M. Surface-active agents (surfactants) are mixed
with the powder. This will help the mixture to become wettable in water;
otherwise, the water insoluble fungicide will be only in suspended form in
water. Wetting agents and dispersion agents are added to the mixture to assist in particle suspension during application. Other adjuvants such as stickers and UV filters are also added to improve persistence. Wettable powders
form a suspension rather than a true solution when added to water. Many
fungicides are formulated as wettable powders. Chlozolinate, cufraneb,
cymoxanil, cyprofuram, dichlone, fenaminosulf, ferbam, fosetyl-aluminium,
fluoromide, pimaricin, triflumizole, and tolyfluanid are formulated only
as wettable powders. Carbendazim, carboxin, chlorothalonil, copper oxychloride, copper sulfate, cuprous oxide, dichlofluanid, dicloran, diniconazole, ditalimfos, dithianon, dodine, etaconazole, etridiazole, fenaminosulf,
fenarimol, fentin acetate, fentin hydroxide, folpet, flutolanil, fluotrimazole,
fthalide, furalaxyl, isoprothiolane, kasugamycin, mancozeb, maneb, mepronil,
metalaxyl, myclobutanil, oxine-copper, oxycarboxin, penconazole, pencycuron, pentachlorophenol, pimaricin, prochloraz, procymidone, pyrifenox,
pyroquilon, thiabendazole, thiophanate, thiophanate-methyl, thiram, tolclofosmethyl, tolyfluanid, triadimenol, triforine, zineb, and ziram are mostly formulated as wettable powders. Several wettable powder trade products are
available. Trade names and suppliers include Adagio (chlorothalonil+
mancozeb, PBI Agrochemicals), Carbendazim (carbendazim, High Kite),
Clortocaffaro Ramato (chlorothalonil+copper oxychloride, Caffaro), Cosan
(wettable sulfur, Hoechst), Dithane M-45 (mancozeb, Rohm and Haas),
Fungitex (thiram, Protex), Gilmore Benomyl (benomyl, Gilmore), Gilmore
Enidod (dodine, Gilmore), Hymush (thiabendazole, Agrichem), KOP-Hydroxide 50 (copper hydroxide, Drexel), Microcop 50 (copper oxychloride,
Probe), M.S.S. Patafol (mancozeb+ofurace, Mirfields), Neoram (copper
oxychloride, Caffaro), Quintozene wettable powder (quintozene, RhonePoulenc Environmental), Rovral (iprodione, Rhone-Poulenc), SF-101 50
SF (ditalimfos, Intermed), Suffa 90 (micronized wettable powder sulfur,
Drexel), Sulfex (wettable sulfur, Excel), Topsin M (thiophanate-methyl, Elf
Atochem), Triadimefon (triadimefon, High Kite), Trimanex (mancozeb,
Protex), Vinclozolin (vinclozolin, High Kite), Virolex (carbendazim, Protex),
and Vitigran (copper oxychloride, Hoechst).
Dustable Powder (Dust)
Dustable powder formulations are similar to wettable powders, as both
are manufactured by grinding the fungicide, together with a solid diluent, in
a ball mill. The particle size of the dust formulations is about 10 M. Particle size is very important. If the diameter is too small, the particles will coagulate, and if the particle size is too large, the activity of it will be significantly reduced. Mercurous chloride is available only as a dust formulation.
Dust formulations of copper oxychloride, dichlofluanid, dicloran, ditalimfos,
dodine, edifenphos, etridiazole, folpet, flutolanil, fthalide, hymexazol, iprobenfos, isoprothiolane, kasugamycin, mancozeb, mepronil, pencycuron,
procymidone, tecnazene, tolclofos-methyl, sulfur, and zineb are available.
Dust formulation is liable to drift, and it may drift long distances from the
treated area, even when wind velocities are low. Trade names of some
dustable dusts are Fluidosoufre (powdered sulfur, Elf Atochem Agri.), Arena 6
(tecnazene, Tripart Farm Chemicals), Arena Plus (tecnazene+ carbendazim,
Tripart Farm Chemicals), Atlas Tecnazene 6 percent dust (tecnazene, Atlas), and Maneb 80 (maneb, Pennwalt).
Driftless (DL) Dust

Dustable dust is subject to drift. To avoid this, DL dusts have been developed. The particle size of DL dust (20 to 30 m) is bigger than conventional
dust (10 M). The bigger particles are less likely to drift than finer particles.
Furthermore, the secondary aggregation of the particles is achieved by addition of paraffin, alkyl, or alkylphenyl ether phosphate as an aggregating
agent, thus producing driftless formulations. DL formulations are used in
the control of rice diseases in Japan.
Emulsifiable Concentrate
In emulsifiable concentrate formulations, active ingredients are dissolved in organic solvents such as xylene and cyclohexane. These solvents
are not soluble in water, so emulsifiers are added to the formulation. Emulsifers, which are partly hydrophilic and partly lipophilic, enable the formation of a homogeneous and stable dispersion of small globules of the solvent
in water. Wetting agents, surfactants, spreaders, and stickers are also included
in emulsifiable concentrate formulations. Dinobuton, dodemorph, fenpropimorph, methyl isothiocyanate, phosdiphen, and tridemorph are formulated
only as emulsifiable concentrates. Emulsifiable concentrate formulations of
cuprous oxide, dichlorophen, diniconazole, ditalimfos, edifenphos, ethirimol,
etridiazole, fenarimol, flusilazol, fluotrimazole, hymexazol, iprobenfos,
isoprothiolane, metalaxyl, myclobutanil, oxycarboxin, penconazole, prochloraz, propiconazole, pyrifenox, tolclofos-methyl, triadimenol, and triforine are available. Trade names of some emulsifiable concentrates are
Nustar (flusilazole, DuPont), Point Imazalil (imazalil, Point), Tridemorph
(tridemorph, 750 g/liter emulsifiable concentrate, High Kite), and Mirage 45
EC (prochloraz, Protex).
Water-Soluble Powder
In water-soluble powder formulation, the active ingredient is finely
ground. The formulation may contain a small amount of wetting agent to assist solution of the ground active ingredient in water. When the formulation
is added to water in the spray tank, it dissolves immediately. Copper sulfate
and 8-hydroxyquinoline sulfate are the important water-soluble powder formulations. Terraneb SP Turf Fungicide (chloroneb, Kincaid) is an important
water-soluble commercial product.
Soluble Concentrate
The active ingredient in a soluble concentrate formulation is soluble in
water and is formulated either with water or with a solvent such as alcohol,
which mixes readily with water. This formulation forms a true solution in
water in the spray tank. Soluble concentrate formulations of dichlorophen,
dimethirimol, guazatine, 8-hydroxyquinoline sulphate, nabam, propamocarb, and propiconazole are available.
Suspension Concentrate
A suspension concentrate formulation is similar to a soluble concentrate,
but the active ingredient is only partially soluble in water. The active ingredient is ground to a fine powder (about 5 M), suspended in either water or
in an organic liquid, and then blended with a solid inert material and suitable
adjuvants. The efficacy of suspension concentrates depends on the type
of adjuvants added to them. Suspension concentrate formulations form suspension in water in the spray tank. Flutriafol is available only in this form.
Carboxin, dithianon, drazolon, ethirimol, fenarimol, fentin acetate, folpet,
fthalide, hexaconazole, mepronil, oxine-copper, procymidone, thiophanate,
and tolclofos-methyl are available as suspension concentrates. Rovral Flo
(iprodione, Rhone-Poulenc) is the trade name of a suspension concentrate.
Water Miscible Concentrate
This type of concentrate formulation is miscible with water. Banol
(propamocarb, AgrEvo) is the commercially available water miscible concentrate.
Flowable Formulations
Flowable formulations are also called water-dispersible suspensions.
The active ingredients in these formulations may not be soluble in water or
in commonly available organic solvents. The active ingredient is impregnated on a diluent such as clay and milled to an extremely fine powder. The
powder is then suspended in a small amount of liquid so that the resulting
formulation is thick, like paste or cream. The flowables are mixed with water to form suspensions in the spray tank. Flowables are similar to wettable
powders but are in suspension. They combine the benefits of both wettable
powders and emulsifiable concentrates. Flowable formulations are available as flowable concentrate (carboxin, iprodione), flowable suspension
(mancozeb, maneb, copper sulfate), and flowable liquid (mancozeb, fenbuconazole). Dry flowables (maneb, carbendazim) are also available. Flowables in general are very finely divided powders.
Trade names of some flowable formulations of fungicides are Agrichem
Maneb Dry Flowable (maneb, Agrichem), Anchor FL (oxadixyl, Gustafson), Captan 4L Flowable (a flowable formulation containing four pounds
of captan per gallon, Drexel), Captan 4 Flowable Seed Protectant (a flowable fungicide used for seed treatment, Drexel), Captan Plus Molybdenum
Flowable Liquid Seed Treatment for Soybeans (captan+molybdenum, Drexel),
Carbate Flowable (carbendazim, Pan Britannica), Chloroneb 300 Fungicide
Flowable (chloroneb, Kincaid), Clortocaffaro Flow (chlorothalonil, Caffaro), Cuproxat Flowable (copper sulfate tribasic, Agrolinz), Delsene (a dry
flowable carbendazim, DuPont), Dithane Dry Flowable (mancozeb, Pan
Britannica), Enable (fenbuconazole, liquid flowable, Rohm and Haas),
Extratect Flowable (thiabendazole+imazalil, MSD Agvet), Gilmore Carbendazim 750g DF (dry flowable carbendazim, Gilmore), Gustafson Baytan 30 (a flowable systemic seed treatment triadimenol fungicide, Gustafson), Imber (flowable sulfur, Tripart Farm Chemicals), KOP-300 (copper
sulfate tribasic, Drexel), Legion (carbendazim+maneb, Tripart Farm Chemicals), Manzate 200 DF (mancozeb dry flowable formulation, DuPont),
Nustar (flusilazole dry flowable formulation, DuPont), Rizolex Flowable
(tolclofos-methyl, AgrEvo), Pasta Caffaro (copper oxychloride, 25 percent
copper metal in flowable formulation, Industrie Chimiche Caffaro), Rovral
(iprodione, oily and aqueous flowable concentrates, Rhone-Poulenc), RTUBaytan-Thiram (triadimenol+thiram, Gustafson), RTU Flowable Soybean
Fungicide (thiram+thiabendazole, Gustafson), RTU-PCNB (quintozene,
Gustafson), RTU-Vitavax-Thiram (carboxin+ thiram, Gustafson), Suffa
(flowable 6 pound elemental sulfur, Drexel), Suffa 8 (flowable 8 pound elemental sulfur, Drexel), Super-Tin 4L (flowable fentin hydroxide, Chiltren),
Tecto Flowable Turf Fungicide (thiabendazole, Vitax), That Flowable Sulfur (sulfur, Stoller), Thiovit (dry flowable micronised sulfur, Sandoz),
Topsin M (thiophanate-methyl, Flowable, Elf Atochem), Victor (carbendazim+chlorathalonil+maneb, Tripart Farm Chemicals), Vitavax-200
(carboxin+thiram, Gustafson), Vitavax-30 C (carboxin, Gustafson), Vitavax34 (carboxin, Gustafson), Vitavax-Extra (carboxin+imazalil+thiabendazole, Gustafson), Vitavax- PCNB (carboxin+quintozene, Gustafson), WackerKupferkalk (copper oxychloride, Wacker), Flowable Sulphur (sulfur, CMI),
Ziram Flowable (ziram, Elf Atochem), and Ziram Dry Flowable (ziram, Elf
Atochem).
Water-Soluble Bag
Some wettable and soluble powders are marketed in water-soluble bags.
When formulations in soluble bags are placed directly in the spray tank, the
bags dissolve. This type of packaging eliminates the need to measure the
fungicide for addition to the spray tank. The trade names of fungicidecontaining water-soluble bags are Contact 75 (chlorothalonil, ISK Biotech)
and Topsin M (thiophanate-methyl, water-soluble bag, Elf Atochem).
Water-Soluble Granule
Granules are very much like dusts except that the inert materials are
much larger. Size of granule formulations ranges from 300 to 1,700 M.
Liquid formulations of the active ingredient are added to particles of clay or
other porous materials such as walnut shells and corn cobs. Granular formulations are used exclusively for soil treatment. Granular formulations have
the merits of little scatter and small loss. They are soluble in water. When
applied to soil, the active ingredients are released gradually from the inert
material. Granular formulations of flusilazol, furalaxyl, hexaconazole, iprobenfos, isoprothiolane, kasugamycin, metalaxyl, pyroquilon, and tecnazene
are available in the market. Trade names of some granular formulations are
Arena Granules (tecnazene, Tripart Farm Chemicals), Atlas Tecgran 100
(tecnazene, Atlas), Bygran F (tecnazene+organo iodine, Wheatley), Bygran
S (tecnazene, Wheatley), Hystore 10 (tecnazene, Agrichem), Moncut (flutolanil, Nihon Nohyaku), and Thiovit (micronized wettable granular sulfur
fungicide, Pan Britannica).
Water-Dispersible Granule
Water-dispersible granule formulations are similar to water-soluble granules. However, the active ingredient is not easily soluble in water. Waterdispersible formulations of copper oxychloride, cuprous oxide, and pentachlorophenol have been developed. Trade names of some water-dispersible
granules are Dithane DF (mancozeb, Rohm and Haas), Kumulus DF (sulfur,
BASF), Microthiol Special (micronized granular sulfur, Elf Atochem North
America), Penncozeb WDG (mancozeb, Cyanamid), Polyram DF (metiram
complex, BASF), Recop (copper oxychloride, Sandoz), and Topsin M (thiophanate-methyl, water dispersible granule, Elf Atochem).
Microgranule
Microgranular formulations of sulfur, fthalide, and iprobenfos have been
developed. The particle size of microgranules is 180 to 710 M. This formulation prevents drift of the active ingredient and gives uniform distribution. Trade names of some microgranule fungicides are Solfa (a free flowing
microgranule of sulfur, Atlas, U.K.), and Copper Sandoz (a water dispersible microgranular cuprous oxide fungicide, Sandoz Agro). Fthalide
and iprobenfos are also available as microgranule formulations.
Liquid Fungicide
Some fungicides have been developed as liquid fungicides. Liquid formulations of dodine, kasugamycin, lime sulfur, and ziram are available.
Trade names of some liquid fungicides are Bencarb (carbendazim, Productos
OSA), Impact (flutriafol+carbendazim+chlorothalonil+pyrazophos, Zeneca),
Hy-TL (thiram+thiabendazole, Agrichem), Jupiter (hexaconazole+fenpropidin, Zeneca), Mascot Contact Turf Fungicide (vinclozolin, Rigby Taylor),
Mascot Systemic Turf Fungicide (carbendazim, Rigby Taylor), MilcurbSuper (ethirimol, Zeneca), Mildothane Liquid (thiophanate-methyl, Hortichem), Mildothane Turf liquid (thiophanate-methyl, Rhone-Poulenc
Environmental), Milgo E (ethirimol, Zeneca), and Pennsuc S (fentin hydroxide+sulfur, Elf Atochem Agri).
Aqueous Suspension
In this formulation, the active ingredient is formulated as aqueous suspension.Trade names of some aqueous formulations are Cuperhidro (copper oxychloride, Grupo Bioquimico Mexicano), Cupertron (copper oxychloride, Grupo Bioquimico Mexicano), and Metacid 400 TS (thiram,
Grupo Bioquimico Mexicano).
Aqueous Solution
Dimethirimol and propamocarb have been developed as aqueous solution formulations.
Oil Dispersible Powder
Some fungicides have been formulated as oil dispersible powders. Trade
names of oil dispersible powders are Benlate OD (benomyl, DuPont) and
Gilmore Benomyl (benomyl, Gilmore).
Oil-Miscible Liquid
Oil miscible formulations of hexaconazole and pentachlorophenol are
available.
ULV Formulation
An ultralow-volume (ULV) formulation is sprayed without any dilution,
at the rate of one-half gallon per acre. The formulation may contain only the
active ingredient or the active ingredient in a small amount of solvent. Special application equipment is needed to spray ULV formulations. Several
acres of the crop can be sprayed with a small amount of liquid with this formulation. Turbair Rovral (iprodione, Turbair) and Kasugamycin ULV liquid are the commercially available ULV fungicide formulations.
Oily Formulation
The commercially available oily formulation is Vondozeb (maneb+zineb,
Elf Atochem). It is mainly used in horticultural crops.
Paste Formulation
Some fungicides are developed as paste formulations. They are mainly
used for treating cuttings and pruning injuries. Copper oxychloride, oxinecopper, and ziram pastes are available.
Dry or Slurry Seed Treatment
Several fungicides have been formulated specifically for seed treatment.
Seed treatment wettable powder formulations of carboxin, etaconazole,
etridiazole, fenfuram, fuberidazole, furmecyclox, guazatine, hexachlorobenzene, hymexazol, mepronil, metalaxyl, metiram, oxine-copper, pencycuron, phenylmercury acetate, tolclofos-methyl, and triadimenol are available. Trade names of some dry/slurry seed treatment formulations available
in the market are Abavit (prochloraz+carboxin, Hoechst), Aliette III WG
(fosetyl+captan+carbendazim, Rhone-Poulenc), Apron Dry (metalaxyl, Gustafson), Apron-FL (metalaxyl, Gustafson), Baytan (triadimenol, Bayer),
Benit Universal (propiconazole+imazalil+thiabendazole, Novartis), Benlate
T (benomyl+thiram, DuPont), Beret (fenpiclonil, Novartis), Captan 30-DD
(fungicidal seed protectant [captan] with colorant, Gustafson), Captan 300
(fungicidal seed protectant without colorant, Gustafson), Carboxin (carboxin,
Melchemie), Celest (fludioxonil, Novartis), Cupromox (cuprous oxide,
Universal Crop), Dividend (difenoconazole, Novartis), Elanco Seed Treat-
ment (nuarimol+imazalil, DowElanco), D-264 Captan Seed Treatment

(captan+diazinon, Drexel), Emisan 6 (2-methoxyethyl mercury chloride,
Excel), Enhance (carboxin+captan, Gustafson), Enhance Plus (carboxin
+maneb+lindane, Gustafson), Ferrax (flutriafol+ethirimol+thiabendazole,
Zeneca), Fongorene (pyroquilon, Novartis), Gambit (fenpiclonil, Novartis),
Gustafson Captan 400 D (captan with red dye, Gustafson), Gustafson
Captan 400 (captan without red dye, Gustafson), Kemikar (carboxin, Kemira
Agro OY), Lindex-Plus F5 Seed Treatment (lindane+thiram+fenpropimorph, DowElanco), Magnate (imazalil, Makhteshim-Agan), Nordox SD45 (cuprous oxide, NORDOX), Panoctine (guazatine, Rhone-Poulenc),
Pentaclor 600TS (quintozene, Grupo Bioquimico Mexicano), PMA Technical (phenyl mercury acetate, Excel), Point Carboxin (carboxin, Point),
Pomarsol (thiram, Bayer), Prelude (prochloraz, Hoechst), Prelude SP
(prochloraz+manganese chloride+carbendazim, Hoechst), Prevail (carboxin+
metalaxyl+quintozene, Gustafson), Quinolate (oxine-copper, Novartis),
Quinondo WP (oxine-copper, Agro-Kanesho), Rapcol TZ (furathiocarb+
metalaxyl+thiabendazole, Novartis), Raxil (tebuconazole, Bayer), Real
(anthraquinone+triticonazole, Rhone-Poulenc), Seed Tox (phenyl mercury
acetate, AIMCO), Sibutol (bitertanol+fuberidazole, Bayer), Spectro
(difenoconazole, Novartis), Spotless (diniconazole, Valent), 42S Thiram
Fungicide (thiram, Gustafson), Systhane (myclobutanil, Rohm and Haas),
Thiram 50 WP (thiram, Gustafson), Tops-5 (thiophanate-methyl, Gustafson), Trimanoc (mancozeb, Elf Atochem), Trimangol (maneb, Elf Atochem),
Trimisem (nuarimol, DowElanco), Trimisem Total (nuarimol+maneb+
lindane+anthraquinone, DowElanco), Triple-Noctin L (thiram+molybdenum, Gustafson), Tripomol (thiram, Elf Atochem), and Vincit (flutriafol,
Zeneca).
Liquid Seed Treatment
Liquid formulations of seed treatment fungicides have also been developed. Etaconazole, ethirimol, fenfuram, guazatine, methoxyethylmercury
acetate, phenylmercury acetate, prochloraz, and triforine are available as
liquid seed treatment formulations. Trade names of some liquid formulations are Agrichem Flowable Thiram (thiram, Agrichem), Captan Plus Molybdenum Flowable Liquid Seed Treatment for Soybeans (captan+molybdenum, Drexel), Hydraguard (thiram+gamma-HCH, Agrichem), Hysede
FL (gamma-HCH+thiram, Agrichem), Hy-Vic (thiram+thiabendazole, Agrichem), Rappor (guazatine, DowElanco), and Rappor Plus (guazatine+
imazalil, DowElanco).
Emulsifiable Seed Treatment

Emulsifiable formulations for seed treatment are available. Gustafson
Terracoat LT-2N (quintozene, Gustafson) is the commercially available
emulsifiable seed treatment formulation.
Flowable Seed Treatment
Several flowable formulations have been developed for seed treatment.
Trade names of some of them are Gustafson Vitavax-Thiram-Lindane
(carboxin+thiram+lindane, Gustafson), Gustafson Baytan 30 (a flowable
systemic seed treatment triadimenol fungicide, Gustafson), Chloroneb 300
Fungicide Flowable (chloroneb, Kincaid), Captan 4 Flowable Seed Protectant (a flowable fungicide used for seed treatment, Drexel), Rival (captan+
quintozene+thiabendazole, Gustafson), RTU Flowable Soybean Fungicide
(thiram+thiabendazole, Gustafson), RTU-PCNB (quintozene, a flowable
seed treatment, Gustafson), RTU-Vitavax-Thiram (carboxin+thiram, Gustafson), Vitavax-30C (carboxin, Gustafson), Vitavax-34 (carboxin, Gustafson), Vitavax-200 (carboxin+thiram, Gustafson), Vitavax-Extra (carboxin+
imazalil+thiabendazole, Gustafson), and Vitavax-PCNB (carboxin+PCNB,
Gustafson).
Soil Application FungicideWettable Powder
Some formulations have been developed specifically for application to
soil. The wettable powder formulations for soil application are Fongarid
(furalaxyl, Novartis), Gilmore PCNB (quintozene, Gilmore), Botran (dicloran, Hoechst), Ipam (metham-ammonium, soil sterilant, Chemol), Pentaclor 600F (quintozene, Grupo Bioquimico Mexicano), and Terraclor 20D
(quintozene, Hortichem).
Soil Application FungicideFumigant
Fumigants are applied to soil to control soilborne pathogens. They are either in liquid or gaseous form. Trade names of some fumigants are BasamidGranular (dazomet, BASF), Brom-O-Gas (methyl bromide+chloropicrin,
Great Lakes), Chlor-O-Pic (chloropicrin, liquid, Great Lakes), Di-Trapex N
(methyl isothiocyanate+dichloropropene, liquid fumigant, Hoechst), Methyl
Bromide (methyl bromide, gas, Melchemie and Marman), Sistan (methamsodium, liquid fumigant, Universal Crop), Unifume (metham-sodium, liquid
fumigant, Universal Crop), Vapam (metham-sodium, Zeneca), and Vorlex

(methyl isothiocyanate+dichloropropane, Hoechst).
Coating Agent
Octhilinone and 2-phenylphenol are used as coating agents. They can be
used for control of postharvest diseases of fruits by impregnation of fruit
wrappers and crates or by application as a wax directly to the fruit. They can
also be used to coat pruning cuts. Mercuric oxide is used as a wound
protectant.
CLASSIFICATION OF FUNGICIDES
More than 113 active ingredients have been registered as fungicides
worldwide. Grouping of fungicides is needed to help the actual user (farmer)
make quick decisions to select a suitable fungicide to control a particular
disease. Three important criteria used to classify fungicides are their chemical strucures, their type of mobility action, and their biochemical modes of
action. Fungicides are broadly divided into two groups based on their mobility in host tissues: systemic and nonsystemic.
Systemic Fungicides
Systemic fungicides penetrate into plant tissue and show apoplastic mobility (movement within cell walls and xylem tissues), symplastic mobility
(movement through plasmadesmata from cell to cell through phloem tissues),
or both apoplastic and symplastic mobility in plants. However, apoplastic
movement alone is common. Most systemic fungicides exhibit acropetal movement (redistribution toward the plant apex or leaf margins); basipetal movement (from plant apex to bottom) is rare. Systemic fungicides mostly move in
the transpiration stream. When these fungicides are applied to the roots of
plants, the chemicals may be translocated over a long distance to protect
young top leaves against pathogens. Some systemic fungicides possess
translaminar activity. When these compounds are applied to the upper surface of a leaf, they can control disease on the lower side of the leaf, and vice
versa. Very few systemic fungicides move in both directions. Fosetylaluminium has been shown to move through both xylem and phloem. Some
systemic fungicides such as triadimefon may produce vapors with fungicidal activity and act at large spreading areas in the foliage. Systemic fungicides may have protectant (protection against sporulation, spore germin-
ation, appressorium formation, and prevention of penetration), curative

(suppression of postinfection and presymptomatic phase of infection and
visible effects of symptoms), and eradicant (suppressive action on the postsymptomatic stage by killing the pathogen in the host) action.
Nonsystemic Fungicides
Nonsystemic fungicides are also called surface active, surface protectant,
or contact fungicides. They do not penetrate the plant surface, and they are
not translocated within the plant. On application, they remain on the surface
of the plant, and redistribution on the foliage of the crop may occur through
the action of dew or rainfall. Nonsystemic fungicides can also be called
protectant fungicides, as they are applied prophylactically to the crop. They
do not have curative or eradicant action and should be applied before infection of the pathogen occurs. These fungicides may prevent fungal spore germination, appressorium formation, and penetration of host tissues. Nevertheless, once infection is established, these fungicides may not have any
function. However, they cannot be called protectant fungicides, as several
systemic fungicides also have protectant action. Some systemic fungicides
such as quinoxyfen inhibit appressorium formation on leaf surfaces. Another systemic fungicide, tricyclozole, inhibits melanization of appressoria
and the subsequent formation of the infection apparatus of the fungi. Some
systemic fungicides such as probenazole and fosetyl-aluminium may act
through induction of the hosts defense mechanisms and act as protectants.
Only a few systemic fungicides have protectant action, but all nonsystemic
fungicides have protectant action.
Fungicide Families
Fungicides are further subdivided into families based on their chemical
structure. The important chemical families are given in the following list.
Names of the chemical families are followed by the common names of fungicides included in those families.
Acetamidecymoxanil, ofurace, metominostrobin
Acylalaninebenalaxyl, furalaxyl, metalaxyl
Amino acid amide carbamateiprovalicarb
Anilinopyrimidinepyrimethanil, cyprodinil
Antibiotickaugamycin, Blasticin-S, pimaricin, polyoxins,
validamycin A, cycloheximide
Benzenediazosulphonatefenaminosulf
Benzimidazole, MBCbenomyl, carbendazim, fuberidazole,

thiabendazole, thiophanate, thiophanate-methyl
Biphenyl2-phenylphenol
Butaylaminesec-butylamine
Carbamatepropamocarb, propineb, thiophanate, thiophanatemethyl, diethofencarb, iprovalicarb, methasulfocarb
Carboxamidecarboxin, oxycarboxin, fenfuram, benodanil,
mepronil, flutolanil, furmecyclox, fenhexamid, ethaboxam,
carpropamid
Chlorinated hydrocarbonchloroneb
Chlorinated nitroanilinedicloran
Chlorophenylchlorothalonil
Dicaboximideiprodione, procymidone, vinclozolin
Dithiocarbamatecufraneb, ferbam, mancozeb, maneb, metiram,
metham-sodium, zineb, ziram, thiram
Dithiolaneisoprothiolane
Guanidinedodine, guazatine
Imidazoleimazalil, prochloraz, triflumizole, perfurazoate
InorganicBordeaux mixture (tribasic copper sulfate), copper
oxychloride, copper oxide, copper hydroxide, sulfur, lime sulfur,
mercurous chloride, mercuric oxide
Isothiazolineocthilinone
Isothiocyanatemethyl isothiocyanate
Isoxazoledrazoxolon, hymexazol
Morpholinedodemorph, fenpropimorph, tridemorph
Nitro compoundbinapacryl, bronopol, chloropicrin, dinobuton,
dinocap, DNOC, nitrothal-isopropyl
Organoaluminiumfosetyl-aluminium
Organochlorinehexachlorobenzene, pentachlorophenol,
quintozene, tecnazene
Organomercurymethoxyethylmercury acetate, phenylmercury
acetate
Organophosphorousditalimfos, edifenphos, iprobenfos,
phosdiphen, pyrazophos, tolclofos-methyl
Organotinfentin acetate, fentin hydroxide
Oxathiinoxycarboxin, carboxin
Oxazolidinechlozolinate, oxadixyl
Oxazolidinonefamoxadone
Phenoxyquinolinequinoxyfen
Phenylpyrrolefenpiclonil, fludioxonil
Phthalic acidfthalide
Phthalimidecaptan, captafol, folpet

Piperazinetriforine
Piperidinefenpropidin
Pyridinebuthiobate, pyrifenox
Pyrimidinebupirimate, nuarimol, dimethirimol, ethirimol,
fenarimol
Pyrrolefluoromide
Pyrroloquinolinepyroquilon
Quinoline8-hydroxyquinoline sulphate, oxine-copper
Quinonedichlone, dithianon, chloranil
Quinoxalinechinomethionat (quinomethionate)
Strobilurineskresoxim-methyl, azoxystrobin
Sulphamidedichlofluanid, tolyfluanid
Thiadiazoleetridiazole
Triazineanilazine
Triazolebitertanol, diclobutrazol, triadimefon, diniconazole,
etaconazole, flusilazol, flutriafol, fluotrimazole, hexaconazole,
myclobutanil, penconazole, propiconazole, triadimenol,
tebuconazole, fluquinconazole, difenoconazole, epoxiconazole,
triticonazole, bromuconazole, cyproconazole, metconazole,
imibenconazole
Ureacymoxanil, pencycuron
Surface protectant fungicides include inorganics, dithiocarbamates, thiadiazoles, quinones, triazines, sulphamides, quinoxalines, nitro compounds,
phthalimides, guanidines, organochlorines, organomercurials, organophosphorous compounds (tolclofos-methyl, edifenphos), organotin compounds,
organozinc compounds, chlorophenyls, chlorinated nitroanilines, urea fungicides, benzenediazosulphonates, furans, pyrroles, fthalides, isoxazole (drazoxolon), acetamide (cymoxanil), imidazole (prochloraz), dicarboximide
(iprodione), isothiazoline, quinolines, biphenyl, and carbamate (propineb).
Systemic fungicides include acetamides (ofurace, metominostrobin),
anilides, anilopyrimidines, benzanilides, benzimidazoles, carbamates, dicarboximide (procymidone), oxazolidine, pyrimidines, pyridines, triazoles,
morpholines, organoaluminium, acylalanines, quinolines, isoxazole (hymexazol), imidazole (imazalil, triflumizole), organophosphorous (iprobenfos,
pyrazophos), dithiolanes, carboxylates, piperazines, pyrolloquinoline, oxathiins, strobilurins, oxazolidinediones, and carboxamides.
(Some chemical families contain both systemic and surface protectant
fungicides. In such families, the names of such fungicides are given within
parentheses.)
MODE OF ACTION OF FUNGICIDES

Disruption of Fungal Cell Functions
Several chemical families of fungicides act by disrupting fungal cell
functions (Ragsdale and Sisler, 1991). These include inorganics, dithiocarbamates, phthalimides, triazines, sulphamides, chlorophenyls, quinones,
and quinoxalines. They are all nonsystemic (surface protectant) fungicides.
Sulfur
Sulfur belongs to the inorganic chemical family. Several formulations of
sulfur (dustable dust, wettable powder, and flowable formulations) are
available. Sulfur in combination with lime, as lime sulfur, is still an important fungicide. Many theories have been evolved to explain the mode of
action of sulfur. The oxidized sulfur theory attributes toxicity of sulfur to
oxidation products such as sulfur dioxide, sulfur trioxide, sulfuric acid,
thiosulfuric acid, or pentathionic acid. The reduced sulfur theory ascribes
toxicity of sulfur to hydrogen sulfide generated by fungal action on sulfur.
The direct action theory, which is now well accepted, is based on the toxic
action of sulfur as such. Sulfur may be involved in biological redox reactions (electron transport system) in the fungal metabolism. Sulfur is reduced
in the electron transport system at a site between cytochrome b and c. Electrons are utilized to reduce sulfur instead of oxygen, and sites of energy conservation (ATP [adenosine triphosphate] generation) are bypassed. Suppression of the ATP generation system results in interference with the
energy supply system, leading to the death of fungal pathogens. Toxicity
could also result from cross-linking of proteins or other cellular components
by free radicals of sulfur or from extensive oxidation of thiol groups, leading to loss of function or structural intregrity of proteins. Sulfur acts against
several biochemical sites in fungal cells. It inhibits respiration, disrupts proteins, and forms chelates with heavy metals within the fungal cells.
Copper Compounds
The inorganic copper fungicides include copper oxychloride, copper sulfate, cuprous oxide, and copper hydroxide formulations. Bordeaux mixture
consists of copper sulfate and lime. Copper ion is the active component in
copper salts. Copper ions in the presence of CO2 from the air and the organic
acids excreted from the plant and/or fungal spores interact to produce the resultant activity. Copper ions are capable of penetrating fungal spores and in-
hibiting their enzyme reactions. They may chelate and remove other essential metals. They may also block or interact with sulfhydryl groups of the
fungal enzymes. Copper ions cause membrane damage resulting in leakage
of metabolites from fungal cells.
Mercury Compounds
Organic and inorganic mercurial fungicides are used exclusively for seed
and soil treatment. All mercurial fungicides have lost their registration in
the United States because of their potential environmental damage and high
toxicity. However, a few mercurial fungicides are still allowed in some
countries. Mercurial ion reacts with enzymes having a reactive sulfhydryl
group.
Tin Compounds
Another group of inorganic fungicides consists of tin compounds. These
compounds inhibit fungal growth by inhibiting oxidative phosphorylation
(suppressing energy production). They may inhibit mitochondrial ATPase
of fungal cells and may bind to protein membrane components and cause
extensive and nonspecific alterations in membrane functions, including permeability. These fungicides bind to several proteins, including those containing sulfhydryl groups of cysteine residues. Thus, tin fungicides may act
at multiple sites in fungal cells.
Dithiocarbamates
Dithiocarbamates are still one of the most important groups of organic
surface-protectant fungicides. The dithiocarbamates are divided into two
groups based on their modes of action: monoalkyldithiocarbamates and
dialkyldithiocarbamates. Monoalkyldithiocarbamates include zineb, maneb,
mancozeb, nabam, metiram, and metham, while dialkyldithiocarbamates
include thiram, ziram, and ferbam. The monoalkyldithiocarbamates possess
a reactive hydrogen on the nitrogen atom which permits the formation of a
highly toxic isothiocyanate. The toxicity of these fungicides appears to be
based on isothiocyanate generation. These fungicides alter permeability of
fungal cells. They inactivate thiol groups of essential enzymes.
Dialkyldithiocarbamates are strong chelating agents. They deprive fungal cells of essential heavy metals. The metals in a chelate complex would
permeate cell membranes more readily than the free metal ions. This will
lead to transport of excess heavy metals into fungal cells. The internally re-
leased heavy metals would inhibit enzyme activity. These fungicides may
form a complex with Cu2+ and bind enzymes or other cellular components
of fungi. The role of Cu2+ in coupling dialkyldithiocarbamates to proteins
has been well demonstrated. This coupling will lead to inhibition of fungal
enzyme activity or of other cellular functions. These dithiocarbamates catalyze rapid incorporation of Cu2+ into the diethyl ester of mesoporphyrin.
The toxicity of dithiocarbamates may result from incorporation of Cu2+ into
a porphyrin precursor of an essential heme-type pigment. Dialkyldithiocarbamates may form mixed disulfides with protein thiol groups and inhibit
enzyme activity. These fungicides inhibit copper-containing enzymes. Thus,
dialkyldithiocarbamates may act at multiple sites in fungal cells and may be
multisite biochemical inhibitors.
Phthalimides
Captan, captafol, and folpet are the important surface-protectant phthalimide fungicides. Captafol and folpet are not marketed in the United States.
These fungicides may oxidize cellular thiol groups and inactivate thiol enzymes. They may reduce the level of glutathione in fungal cells. Respiration
is strongly inhibited by these fungicides. Several enzymes such as glyceraldehyde-3-phosphate dehydrogenase, carboxylase, hexokinase, and aldolase in fungal cells are inhibited by these fungicides. They also inactivate
coenzyme-A. They have multiple sites of action in fungal metabolic systems.
Chlorophenyls
Chlorothalonil is a chlorophenyl fungicide and an important surface
protectant. It markedly depresses the level of soluble and cell-bound thiol
groups in fungal cells. The fungicide inactivates the thiol enzymes alcohol
dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase, and malate
dehydrogenase. It does not inactivate nonthiol enzymes. Its fungicidal
mechanism appears to be based on thiol inactivation.
Quinones
Dichlone and dithianon are the important fungicides in the quinone
group. Dichlone, which is not marketed in the United States, interferes with
the electron transport system in fungal cells. It uncouples oxidation from
phosphorylation in the normal electron transport pathway or diverts electrons through other pathways to oxygen not associated with high-energy
phosphate generation. It also inactivates coenzyme-A and inhibits certain

dehydrogenases and carboxylases of fungal cells. It binds thiol and amino
groups in fungal cells. Dithianon inactivates thiol-dependent enzymes.
Triazines
Anilazine belongs to the triazine group. It reacts with free amino acid and
thiol groups in fungal cells. Its mode of action seems to be similar to that of
phthalimides, quinones, and chlorophenyls.
Sulphamides
Dichlofluanid and tolyfluanid belong to the sulphamide group. They are
also surface-protectant fungicides. Their mode of action is similar to that of
phthalimide fungicides.
Quinoxalines
Chinomethionate (quinomethionate) belongs to the quinoxaline group. It
acts by binding with amino and mercapto groups resulting in the inhibition
of sulfhydryl-containing enzymes in fungal cells.
Thiadiazoles
Etridiazole belongs to the thiadiazole group. It inhibits respiration of fungal cells.
Butylamine
sec-Butylamine interferes with pyruvate dehydrogenase complex and inhibits pyruvate oxidation in fungal cells.
Guanidines
Guanidine fungicides disrupt permeability of fungal cells. Alteration of
membrane permeability may result from disruption of normal anioniccationic interactions in the membrane because of the displacement of membrane cationic groups by firmly bound cationic surfactant molecules. Actions that affect membrane structure can result in enzyme inhibition.
Organochlorines
Quintozene is the important fungicide in the organochloride group. It
probably binds to hydrophobic regions in the fungal cell and interferes with
various processes such as membrane function, meiosis, or cell division processes. It may also affect nuclear processes.
Nitro Compounds
Nitro compounds uncouple oxidative phosphorylation and prevent the
incorporation of inorganic phosphate into ATP without affecting electron
transport in fungal cells. Cell death occurs due to the lack of energy for cellular metabolism.
Chlorinated Nitroanilines
Dicloran belongs to the chlorinated nitroaniline group. Inhibition of protein synthesis has been suggested as the toxic mechanism of dicloran. It affects nuclear stability in fungi and disorganizes cell growth and division.
Inhibition of Sterol Biosynthesis
Triazoles, Imidazoles, Pyridines, Pyrimidines, Piperazines,
Morpholines, Piperidines, Spiroxamines
Several systemic fungicides act by inhibiting sterol synthesis in fungi.
Sterols are commonly found in fungi, and ergosterol is the major fungal
sterol. Ergosterol is involved in the maintenance of membrane integrity, and
reduction in ergosterol synthesis results in membrane disruption and electrolyte leakage in fungal cells. The biosynthetic pathway of sterols involves
several steps. Sterols are formed from acetyl-CoA. Sterol biosynthesis inhibitors can be divided into two major groups based on their primary site of
action in the sterol biosynthetic pathway: (1) sterol C-14 demethylation inhibitors (DMI) and (2) sterol 8,7 isomerase and/or sterol 14 reductase
inhibitors. DMIs contain the most commercially valuable group of fungicides. The chemical families include triazoles, imidazoles, pyridines, pyrimidines, and piperazines. Several triazole fungicides are available on the
market. Imazalil, prochloraz, and triflumizole are the important imidazole
DMIs. Nuarimol and fenarimol are the important pyrimidine DMIs. Buthiobate and pyrifenox are the important pyridines. DMIs inhibit all groups of
fungi except those belonging to chromista and oomycota, as the ergosterol
synthetic pathway is absent in fungi belonging to oomycota. The latter

group of fungi obtains their sterol requirements directly from their hosts
through mycelial uptake. DMIs inhibit sterol C-14 demethylation in fungal
cells through interference with a cytochrome P-450 monooxygenase enzyme. The N atom of the fungicide binds at the position of the P-450 heme
prosthetic group where O2 would normally bind. The lipophilic substituent
of the DMI fungicides binds to the region of the apoenzyme that is structurally associated with a C-14 methyl sterol.
Sterol 8,7 isomerase and/or sterol 14 reductase inhibitors include
morpholines, piperidines, and spiroxamines. Dodemorph, fenpropimorph,
tridemorph, fenpropidin, and spiroxamine are the important sterol 8,7
isomerase and/or sterol 14 reductase inhibitors. The mode of action of
these fungicides is mediated by the interaction of the negatively charged enzyme site and the positively charged nitrogen atom in the fungicide molecule.
Inhibition of Phosphatidylcholine Biosynthesis
Edifenphos, Iprofenphos, Isoprothiolane
These three fungicides appear to have a common mode of action. Phosphatidyl choline is essential for the function of fungal cell membranes, providing a permeability barrier to the movement of ions and macromolecules
and a fluid matrix for the activity of membrane-associated proteins. It is essential for fungal growth. The inhibition of phosphatidyl choline synthesis
reduces its incorporation into membranes and decreases chitin synthase activity. It leads to decreased hyphal growth. Hyphal swelling is common in
fungi treated with these fungicides.
Inhibition of Phosphatidylinositol Biosynthesis
Validamycin
Phosphatidylinositol is required for fungal growth. Inositol-deficient
fungal mutants require a supply of exogenous inositol for normal growth.
These mutants show reduced hyphal growth, increased hyphal branching,
and cell lysis. Its deficiency affects carbohydrate, protein, and nucleic acid
metabolism. It also affects wall-bound biosynthetic enzymes. Validamycin
inhibits phosphatidylinositol biosynthesis in fungal cells, and it is the only
fungicide that shows this mode of action.
Inhibition of Tubulin Synthesis

Benzimidazoles
Benzimidazoles act primarily on cell and nuclear division. Nuclear division is inhibited by the binding of benzimidazoles to the microtubular proteins involved in the synthesis of the mitotic spindle apparatus. Microtubules are formed by the assembly of tubulin, a heterodimeric protein with
subunits as alternating helices of - and -tubulins, which forms an essential part of the fungal cytoskeleton. They are active in spindle formation and
in segregation of chromosomes in cell division. Benzimidazoles show high
affinity for tubulin proteins in fungi. They disrupt mitosis during cell division at metaphase in fungi. The mitotic spindle is distorted, and daughter
nuclei fail to separate. These events lead to fungal cell death. The target site
of benzimidazoles is -tubulin. The modification of a single amino acid resulting from a mutational change in -tubulin confers resistance to benzimidazoles in fungi. Benzimidazoles are highly selective in their action
against fungi. They do not inhibit fungi belonging to oomycota and Alternaria spp., although these fungi also contain -tubulin. This suggests that
binding sites on tubulin may vary among pathogens, and binding at the specific sites alone may lead to the inhibitory action of a specific fungicide.
Inhibition of Nucleic Acid Metabolism
Acylalanines
Metalaxyl, metalaxyl M, and benalaxyl belong to the acylanine group.
They act by inhibiting the synthesis of ribosomal RNA via the RNA polymerase I-template complex. Ribosomal RNA synthesis is selectively inhibited, while transfer RNA and messenger RNA syntheses are not affected.
Protein, DNA, and lipid biosyntheses are affected later to a lesser degree,
but respiration is not affected. This group of fungicides specifically inhibits
fungi belonging to oomycota. The fungicides do not affect sporangia and
zoospore germination, penetration, and formation of primary haustoria but
do affect further hyphal development. The hyphal cells become thickened,
leading to eventual death. The RNA-polymerase I complex is inhibited
which results in accumulation of rRNA precursors, the nucleotide triphosphates that promote the activity of fungal -1,3-glucan synthetase and the
synthesis of cell wall constituents.
Pyrimidines
Ethirimol, dimethirimol, and bupirimate are the important pyrimidine
fungicides. They act through the inhibition of adenosine deaminase, an enzyme in the purine salvage pathway. Adenosine deaminase is not present in
plants but is found in a wide range of fungi. However, the fungicides affect
the enzyme of powdery mildew fungi only.
Isoxazoles
Hymexazol is the important isoxazole fungicide. It inhibits DNA synthesis in fungal cells. RNA and protein synthesis is less affected, and DNA synthesis is regarded as the primary site of action of this fungicide. The other
isoxazole fungicide, drazoxolon, inhibits oxidative phosphorylation in fungi.
Inhibition of Respiration
Carboxamides
Carboxin and oxycarboxin are the important fungicides in the carboxamide group. These fungicides are inhibitory to fungi belonging to basidiomycota. They inhibit succinate dehydrogenase complex in the respiratory
chain in fungi. Carboxin blocks membrane-bound succinate-ubiquinone
oxidoreductase activity in the mitochondrial electron transport chain. The
iron-sulfur cluster S3 complexed with small ubiquinone binding polypeptide(s) in a phospholipid environment appears to be the carboxin receptor in the succinic dehydrogenase complex. It also inhibits glucose and acetate oxidative metabolism. RNA and DNA syntheses are also inhibited,
which may be due to a lack of cellular energy resulting from the inhibition
of respiration.
Benodanil, fenfuram, mepronil, fluotanil, and furmecyclox also inhibit
respiration in fungi, and their mode of action is similar to carboxin.
Strobilurins
Kresoxim-methyl and azoxystrobin are the important fungicides in the
strobilurin group. They are active in the inhibition of electron transfer in
complex III of the mitochondrial electron transport chain.
Famoxadone
Famoxadone inhibits the function of the ubiquinol cytochrome c oxidoreductase enzyme at complex III.
Inhibition of Chitin Biosynthesis
Polyoxins
Chitin is a component of several fungi; however, it is absent in fungi belonging to oomycota. Chitin synthase (chitin-UDP-N-acetylglucosaminyl
transferase) transfers N-acetylglucosamine from UDP-N-acetylglucosamine
to the chitin polymer. Polyoxins inhibit chitin synthesis. Polyoxins are similar to UDP-N-acetylglucosamine and are competitive inhibitors of chitin
synthase. Polyoxins induce swelling and bursting of spore germ tubes and
hyphal tips, reactions that kill the fungus.
Inhibition of Melanin Biosynthesis
Tricyclazole and Pyroquilon
The pigment melanin is important in fungal pathogenicity. Melanization
of fungal appressorial walls is essential for the development of infection
hyphae and penetration of the host epidermis. Tricyclazole and pyroquilon
inhibit melanin synthesis and suppress fungal infection.
Carpropamid
Carpropamid inhibits scytalone dehydratase (an enzyme involved in fungal melanin biosynthesis), effectively preventing the pathogen from penetrating the host plant. The fungicide has a dual mode of action. In addition to
inhibiting melanization, it also acts as an inducer of disease resistance. It induces lignification in rice plants. It also induces increased accumulation of
the phytoalexins momilactone A and sakuranetin (Thieron et al., 1998).
Inhibition of Protein Synthesis
Kasugamycin and Blasticidin S
Both antibiotics are inhibitors of protein synthesis in fungal cells. Kasugamycin binds to the 30S ribosomal subunit and inhibits protein synthesis.
Blasticidin S interacts with the larger ribosomal subunit and blocks the
binding site for incoming aminoacyl-tRNA molecules.
Inhibition of Biosynthesis of Lytic Fungal Enzymes

Anilinopyrimidines
Cyprodanil and pyrimethanil are the important anilinopyrimidine fungicides. They inhibit production and secretion of fungal enzymes involved in
plant cell wall penetration. These enzymes include pectic enzymes, cellulases, and proteinases.
Inhibition of General (Undefined) Metabolism of Fungi
Organophosphorous Compounds
Triamiphos, ditalimfos, and pyrazophos inhibit fungal spore germination
and appressorium formation.
Hexachlorobenzene, Pentachlorophenol, Tecnazene, Dicloran, Chloroneb
They are organochlorine and related compounds. They induce swelling
of hyphal tips and germ tubes, leading to lysis. They do not inhibit spore
germination. They affect membrane function and induce mitochondrial aberrations.
Phenylpyrroles
Fenpiclonil and fludioxonil are the important fungicides in the phenylpyrrole group. They cause several changes in fungal metabolism. They inhibit glucose phosphorylation in fungal cells.
Propamocarb
Propamocarb affects the fungal cell membrane, inducing leakage of
phosphates, carbohydrates, and proteins. It inhibits only oomycota fungi.
Cymoxanil
Cymoxanil affects fungal metabolic processes. It inhibits nucleic acid
and protein biosynthesis in fungal cells.
Quinoxyfen
Quinoxyfen specifically inhibits powdery mildew fungi. It inhibits appressorial formation.
Dimethomorph
Dimethomorph suppresses cell wall formation in fungi belonging to
oomycota. However, Pythium spp. are not affected by this fungicide.
Fosetyl-Aluminum
Fosetyl-aluminum shows specific activity against fungi belonging to
oomycota. This seems to be the only fungicide that moves through phloem.
It inhibits sporangial formation and zoospore release. It alters fungal cell
wall morphology. It may modify host defense mechanisms.
Dicarboximides
Dicarboximide and related compounds include iprodione, procymidone,
vinclozolin, and chlozolinate. They inhibit conidial germination and mycelial
growth. They induce swelling and bursting of hyphal cells. They alter lipid
metabolism and DNA synthesis in fungal cells. They may cause mitotic instability. Dicarboximides are known to inhibit certain flavin enzymes such
as NADPH-cytochrome c reductase. This action may result in an abnormal
electron flow producing active oxygen species such as H2O2 and superoxide
anion. It may lead to membrane destruction and various nonspecific toxic
effects.
Aminoacid Amide Carbamates
Iprovalicarb belongs to the aminoacid amide carbamate group. It inhibits
the growth of germ tubes of zoospores and sporangia, mycelial growth, and
sporulation of oomycota fungi.
SPECTRUM OF DISEASES CONTROLLED
BY INDIVIDUAL FUNGICIDES
Numerous fungicides have been commercially developed to manage fungal diseases. The efficacy of each fungicide in controlling crop diseases is
given in the following list. The common name of the fungicide alone is given.
The specificity of action of fungicides is normally restricted to the level of
genera of the fungi and not to the species level. For example, a fungicide that
is effective against the powdery mildew fungus Erysiphe graminis will be
effective against all other species of Erysiphe, including E. polygoni, E.
cichoracearum, and E. pisi. Similarly, a fungicide that is effective against
the rust fungus Puccinia graminis will be effective against all other Puccinia species. Hence, the diseases are indicated only by the genera of the
causal fungal pathogens. Furthermore, the specificity of a fungicide appears
to be against the groups of fungal pathogens that cause similar symptoms.
For example, a fungicide that is effective against one powdery mildew fungus, E. graminis, will be effective not only against other species of Erysiphe, but also against other genera of fungi causing powdery mildews, such
as Sphaerotheca, Uncinula, Leveillula, Oidium, Microsphaera, Podosphaera, and Phyllactinia. Names of some specific diseases are also given
in some places; this is mainly to indicate that the particular fungicide is
highly useful in the control of the particular disease. The common names of
the fungicides are given not according to importance but in alphabetical sequence.
Anilazinecontrols early and late blight of potato and tomato; glume
blotch of wheat; anthracnose in cucurbits; Pyrenophora, Botrytis,
Septoria, Colletotrichum, Cercospora, Helminthosporium, and
Alternaria diseases of various crops
Azoxystrobina broad spectrum fungicide; controls powdery mildews,
rusts, and downy mildews; Mycosphaerella, Phytophthora, Phomopsis,
Pyrenophora, Septoria, Rhizoctonia, and Alternaria diseases; also controls Claviceps purpurea and Monographella nivalis diseases;
Aphanomyces root rot of peas; and radish and club root of cabbage
Benalaxylcontrols diseases caused by oomycete fungal pathogens such
as Phytophthora, Plasmopara, Sclerospora, Pseudoperonospora, and
Pythium
Benodanileffectively controls rust diseases; Rhizoctonia diseases;
Typhula in cereals
Benomylcontrols Botrytis diseases; powdery mildews; Fusarium, Verticillium, Sclerotinia, Cercospora, Septoria, Gloeosporium,
Colletotrichum, Venturia, Rhizoctonia, Macrophomina, Ascochyta,
Cephalosporium, Claviceps, Diaporthe, Gibberella, Phomopsis, and
Pyricularia diseases; also controls Plasmodiophora brassicae
Benzothiadiazolecontrols downy mildews; Plasmopara halstedi
Binapacryleffective in control of powdery mildews
Bitertanolcontrols scab and Monilinia diseases of apple and pear; apricot leaf scorch (Gnomonia erythrostoma); black spot of roses; Sigatoka
of banana, powdery mildews and rusts of various crops; also controls
Fusarium, Myrothecium, Phaeoisariopsis, and Monographella diseases
Bordeaux mixturecontrols downy mildews; Phytophthora, Alternaria,
Cercospora, Colletotrichum, Pythium, and Venturia diseases
Bromuconazolecontrols Microsphaera alphitoides; used in cereals

against Pseudocercosporella herpotrichoides and Septoria tritici
Bronopolcontrols bacterial diseases caused by Xanthomonas and
Erwinia; black arm of cotton; also controls Botrytis and Fusarium diseases
Bupirimatecontrols powdery mildews; Pleospora betae
Buthiobatecontrols powdery mildews
Butylaminecontrols Fusarium solani, Helminthosporium solani, Phoma
exigua, and Polyscytalum pustulans diseases
Captafolcontrols downy mildew and black rot of vines; early and late
blight of potato and tomato; controls scab of apple and pear; Botrytis,
Colletotrichum, Corticium, Fusarium, Rhizoctonia, and Sclerotinia diseases; used as a seed treatment to control Pythium and Phoma spp.
Captancontrols scab of apple and pear; Alternaria, Phytophthora,
Pythium, Botrytis, Gloeosporium, Fusarium, Botryodiplodia,
Diaporthe, Gibberella, Glomerella, Macrophomina, Phoma,
Phomopsis, Sclerotinia, and Colletotrichum diseases; brown rots of
cherries, apricots, peaches, plums, and citrus fruits; mostly used as a
seed treatment for the control of seedborne diseases
Carbendazimcontrols Cercospora, Septoria, Colletotrichum, Fusarium,
Rhizoctonia, Macrophomina, Rhynchosporium, Pseudocercosporella,
Botryodiplodia, Botrytis, Ceratocystis, Corticium, Diaporthe,
Erysiphe, Gibberella, Glomerella, Mycosphaerella, and Pyricularia
diseases
Carboxinseed treatment for control of smuts and bunts of wheat, barley,
oats, and sorghum; controls Rhizoctonia solani, Corticium rolfsii,
Stagonospora, and Leptosphaerulina diseases and a wide range of seed
and seedling diseases
Carpropamidsystemic fungicide used as seed treatment and foliar spray
for control of Pyricularia oryzae in rice
Chloralformamidecontrols Leptosphaeria and Ustilago diseases
Chloronebmostly applied as a soil drench or as a seed treatment to control seedling diseases caused by Rhizoctonia, Sclerotium, and Pythium;
snow mold of turf grass
Chloropicrinused as a soil disinfectant for control of soil fungal pathogens
Chlorothalonila broad spectrum protectant fungicide; controls
Cercospora, Septoria, Alternaria, Fusarium, Phytophthora,
Colletotrichum, Glomerella, Pseudocercosporella, Rhizoctonia,
Rhynchosporium, Taphrina, Mycosphaerella, Botryodiplodia,
Sclerotium, Stemphylium, and Botrytis diseases; rusts and powdery mildews
Chlozolinatecontrols Botrytis, Sclerotinia, and Monilinia diseases

Copper hydroxidecontrols bacterial and fungal diseases; citrus canker;
control of many diseases caused by Phytophthora, Alternaria,
Cercospora, Colletotrichum, Fusarium, Rhizoctonia, and Septoria;
downy mildews; apple scab; coffee rust; provides long residual control,
will not readily wash off, and is highly economical
Copper oxychloridecontrols many diseases caused by Phytophthora,
Alternaria, Cercospora, Colletotrichum, Fusarium, Rhizoctonia,
Taphrina, and Septoria; downy mildews; apple scab; coffee rust; asparagus rust; controls bacterial diseases; Pseudomonas syringae pv. tabaci
in tobacco
Copper sulfatecontrols Dutch elm disease by tree injection; phytotoxic
when used alone, but used as a general fungicide when mixed with
lime to form Bordeaux mixture
Cufranebcontrol of downy mildews, early and late blights of potato, apple scab, and black arm of cotton
Cuprous oxidecontrol of downy mildews, rusts, early and late blights of
potato and tomato, and leaf spots caused by Alternaria, Cercospora,
Colletotrichum, and Septoria
Cycloheximidecontrols powdery mildews in ornamentals; rust and leaf
spot diseases of lawn grass
CymoxanilSpecifically effective against fungi belonging to
Peronosporales such as Phytophthora, Plasmopara, and Peronospora;
controls Phoma, Erysiphe, Alternaria, and Fusarium diseases
Cyproconazolecontrols rusts and powdery mildews; Cercospora,
Cochliobolus, Colletotrichum, Fusarium, Gibberella, Mycosphaerella,
Pseudocercosoporella, Rhynchosporium, and Septoria diseases; controls coffee rust
Cyprodinilcontrols apple scab; Botrytis, Sclerotinia, Leptosphaeria, and
Monilinia diseases; Pyrenophora teres, Pseudocercosporella
herpotrichoides, Rhynchosporium secalis, and Mycosphaerella
graminicola diseases of cereals
Cyprofuramspecifically effective against fungi belonging to
Peronosporales such as Pythium, Phytophthora, Plasmopara, and
Peronospora
Dazometsoil sterilant; controls Pythium, Rhizoctonia, and Sclerotinia
diseases
Dichlofluanidcontrols scab of apple and pears; Botrytis diseases on
grapes, tomato, and strawberry; downy mildews; Alternaria and Phytophthora diseases; reduces powdery mildews
Dichlonecontrols scab on apples and pears, brown rot of stone fruit,
and Botrytis cinerea diseases
Dichlorophencontrols Fusarium patch and dollar spot in turf

Diclobutrazolcontrols powdery mildews in cereals; apple, cucurbits,
and vines; rusts in cereals
Diclomezinecontrols Rhizoctonia and Sclerotium diseases in rice,
wheat, peas, and beans
Dicloranhighly effective against Botrytis; controls Monilinia,
Sclerotinia, Sclerotium, and Rhizopus diseases
Diethofencarbcontrols Botrytis cinerea
Difenoconazolecontrols rusts, powdery mildews, and bunts; controls
black as well as yellow Sigatoka and other leaf diseases in banana; diseases caused by Septoria, Stagonospora, Leptosphaeria, Botrytis,
Cercospora, Alternaria, Cochliobolus, Diaporthe, Colletotrichum,
Mycosphaerella, Pseudocercosporella, Setosphaeria, Claviceps,
Diaporthe, Venturia, and Fusarium
Difulmetorimeffective against rose powdery mildew (Sphaerotheca
pannosa) and chrysanthemum white rust (Puccinia horiana)
Dimethirimolsystemic fungicide, absorbed by the roots, with
translocation in the xylem; soil application controls powdery mildews
in cucurbits, tobacco, capsicum, and some ornamentals
Dimethomorphcontrols Phytophthora, Peronospora, and Plasmopara
diseases
Diniconazolevery effectively controls late leaf spot, early leaf spot,
rust, and white mold on peanuts; gives excellent control of powdery
mildews on apples and grapes; controls rusts, smuts, bunts, apple scab,
and Septoria diseases of wheat; also controls Rhizoctonia diseases
Dinobutoncontrols powdery mildews
Dinocapcontrols powdery mildews in cucurbits, vines, tobacco, pome
fruits, and stone fruits
Diphenylaminecontrols apple scald
Disulfotoncontrols coffee rust
Ditalimfoscontrols powdery mildews in pome fruits, cucurbits, and ornamental plants; scab on apples and pears
Dithianonbroad spectrum protectant fungicide; controls scab on apple,
pear, and cherries; downy mildews; rusts; leaf spot diseases caused by
Mycosphaerella, Ascochyta, Glomerella, Diplocarpon, and Didymella;
peach leaf curl; Marssonia leaf spot on poplars; Monilia diseases
DNOCcontrols Phomopsis viticola on vines
Dodemorph acetatecontrols powdery mildews on roses and other ornamental plants
Dodineprotective and curative fungicide; controls scab on apple and
pear; leaf spot diseases of celery, cherries, and strawberries
Drazoxoloncontrols powdery mildews on roses and blackcurrants; controls coffee rust, tea blister blight, and Ganodema disease on rubber;
used as seed treatment for control of Pythium and Fusarium diseases
Epoxiconazolecontrols rusts and powdery mildews, Claviceps purpurea
on rye; Pyrenophora, Colletotrichum, Glomerella, Fusarium,
Leptosphaeria, Septoria, Rhizoctonia solani, and Monographella
nivalis diseases
Ethoxyguincontrols apple scald
Etridiazolecontrols Pythium and Rhizoctonia diseases in cotton, vegetables, ornamentals, and turf
Fenamiphoscontrols Fusarium and Phytophthora diseases
Fenarimolvery effectively controls powdery mildews; also controls apple and pear scab and Pleospora betae
Fenbuconazolea protectant wettable powder fungicide for control of
brown rot of plums and peaches, cherry leaf spot, and rusts
Fenhexamidcontrols Botrytis cinerea and Monilinia fructigena; effective against brown rot and gray mold in sweet cherries
Fenpiclonilcontrols Fusarium, Phoma, Helminthosporium, and
Pyrenophora diseases; also controls Monographella nivalis and
Polyscytalum pustulans diseases; effective against a bacterial disease
caused by Streptomyces; as seed treatment controls seedborne diseases
including silver scurf and stem canker in potatoes
Fenpropidineffective against powdery mildews, rusts, and
Rhynchosporium secalis of cereals
Fenpropimorphcontrols powdery mildews and rusts; controls
Colletotrichum, Septoria, Rhynchosporium, Glomerella,
Mycosphaerella, Pleospora, Pseudocercosporella, and Leptosphaeria
diseases
Fentin acetatecontrols early and late blights of potatoes; Cercospora,
Septoria, Phytophthora, Monilia, Rhizoctonia, Cochliobolus,
Helminthosporium, Pyricularia, and Venturia diseases
Fentin hydroxidecontrols Cercospora, Alternaria, and Phaeoisariopsis
griseola diseases
Fluazinamcontrols late blight of potato, Rhizoctonia, Corticium,
Sclerotinia, Leptosphaeria, Phaeoisariopsis, Plasmodiophora
brassicae, and Rosellinia necatrix diseases; controls the bacterial disease caused by Streptomyces
Fludioxonilcontrols Botrytis, Phytophthora, Fusarium, Sclerotinia, and
Monographella nivalis diseases; controls dollar spot, brown patch, pink
and snow mold in turf; Rhizoctonia in ornamentals
Fluoromidecontrols apple and pear scab, scab of citrus, powdery mildews, Monilia diseases, pink disease of rubber, Alternaria leaf spots,
and anthracnose diseases
Fluotrimazolecontrols powdery mildews of barley, cucumbers, grapes,
and peaches
Fluquinconazolecontrols many cereal diseases; effective in control of
Gaeumannomyces graminis and Mycosphaerella graminicola in wheat
Flurprimidolcontrols Rhizoctonia solani
Flusilazolecontrols powdery mildews, rusts and scab of apples and
pears; Colletotrichum, Glomerella, Monilinia, Leptosphaeria,
Mycosphaerella, Phaeoisariopsis, and Taphrina deformans diseases
Flusulfamidehas bactericidal activity; controls the bacterial pathogens
Clavibacter michiganensis ssp. sepedonicus and Streptomyces scabies;
also controls the fungal pathogen Plasmodiophora brassicae
Flutolanilcontrols rice sheath blight, potato black scurf (Rhizoctonia
solani), cereal Typhula (snow blight) diseases, Corticium diseases, and
turf diseases
Flutriafolcontrols leaf and ear diseases in cereals; as a seed treatment,
controls seedborne diseases of cereals; controls powdery mildews,
rusts, bunts, and scab; Botrytis, Alternaria, Cercospora, Cochliobolus,
Colletotrichum, Glomerella, Leptosphaeria, Mycosphaerella, and
Pleospora betae diseases
Folpetprotectant fungicide; controls downy mildews, powdery mildews,
and scab diseases; Botrytis, Alternaria, Pythium, Rhizoctonia,
Mycosphaerella, and Pestalotiopsis diseases
Fosetyl-aluminiumcontrols diseases caused by fungi belonging to
Peronosporales such as Phytophthora, Pythium, Plasmopora, and
Bremia; Fusarium oxysporum, Fusarium solani, Oidium mangiferae,
Peronosclerospora sorghi, and Pseudoperonospora cubensis diseases
Fthalideeffectively controls rice blast
Fuberidazolecontrols Fusarium culmorum and Monographella nivalis
diseases of cereals
Furalaxylcontrols soilborne diseases caused by Pythium and Phytophthora
Furmecycloxuseful seed treatment for control of Ustilago spp.,
Helminthosporium spp., Septoria spp., Fusarium spp., and Rhizoctonia
spp. in cereals, potato, and cotton
Gramicidin Scontrols Sphaerotheca fuliginea
Guazatine acetateuseful mainly as a seed treatment for the control of
Septoria nodorum, Tilletia caries, Fusarium spp., and
Helminthosporium spp. in cereals; controls Cercospora in groundnut
and pine apple disease in sugarcane; as a postharvest treatment controls
Penicillium digitatum, P. italicum, and Geotrichum candidum in citrus

and postharvest decay of pineapple
Hexachlorobenzeneeffective seed treatment for control of common bunt
and dwarf bunt of wheat
Hexaconazolecontrols rusts, powdery mildews, and scab diseases in apples, pears, vines, vegetables, and other crops
Hexanalcontrols Botrytis cinerea and Penicillium expansum
8-Hydroxyquinoline sulfatecontrols Botrytis cinerea; also used as soil
sterilant for control of soilborne diseases such as damping-off in seed
beds of vegetables
Hymexazolcontrols Fusarium, Pythium, Corticium, and Aphanomyces
diseases
Imazalilcontrols powdery mildews, Fusarium culmorum, Fusarium
solani, Gibberella fujikuroi, Helminthosporium solani, Cochliobolus
sativus, Phoma exigua, and Pleospora betae diseases; storage diseases
of apple, pear, citrus, and banana; also used as a seed dressing, for control of diseases of cereals and cotton; used for control of silver scurf,
gangrene, skin spot, and Fusarium dry rot on ware and seed potatoes
Imibenconazolebroad spectrum fungicide effective against diseases in
fruits, vegetables, and ornamentals; controls apple scab, rust, powdery
mildew, fly speck, sooty blotch, and Alternaria blotch; pear scab and
rust; peach scab; apricot scab; grape anthracnose; citrus scab; peanut
late leaf spot; tobacco powdery mildew; rose black spot, powdery mildew; chrysanthemum rust; and watermelon powdery mildew
Iminoctadinecontrols Botrytis cinerea and Sphaerotheca fuliginea diseases; Fusarium nivale and Fusarium roseum diseases in wheat; rice
blast and brown spot; apple scab, blotch (Alternaria mali), ring rot
(Botryosphaeria berengeriana), and black spot (Diplocarpon mali);
pear black spot, scab, and canker diseases; brown rot and scab of
peach; cucumber powdery mildew; potato early blight; watermelon
anthracnose, powdery mildew, and cottony rot (Sclerotinia
sclerotiorum)
Ipconazolecontrols rice fungal diseases
Iprobenfoscontrols rice blast, sheath blight, and stem rot
Iprodionecontact fungicide, controls Botrytis, Alternaria, Monilinia,
Corticium, Fusarium, Helminthosporium, Phoma, Rhizoctonia,
Sclerotinia, Septoria, Cochliobolus, Diaporthe, Phomopsis, Gibberella,
Glomerella, Leptosphaeria, Pleospora, Uromyces, Pseudocercosporella,
Pseudoperonospora, and Typhula diseases; controls postharvest decay
of peaches, sweet potatoes, and grapes; used as a seed treatment for
control of seedborne fungi
Iprovalicarbshows specific activity against oomycetes; controls

Plasmopara, Phytophthora, and Pythium diseases
Isoprothiolanecontrols blast and stem rot of rice
Kasugamycincontrols fungal and bacterial diseases; useful for the control of rice blast, tomato leaf mold, apple scab, sugar beet leaf spot
(Cercospora beticola), and the bacterial diseases bean halo blight
(Pseudomonas phaseolicola) and potato soft rot (Erwinia carotovora);
also controls Xanthomonas and Clavibacter diseases
Kresoximcontrols Sphaerotheca pannosa in rose, Venturia inaequalis
in apple, Aphanomyces root rot of peas and radish, and club root of
cabbage
Kresoxim-methylcontrols powdery mildews and apple scab;
Mycosphaerella graminicola, Pyrenophora teres, Stagonospora
nodorum, and Monographella nivalis diseases in cereals; Fusarium,
Rhizoctonia, Septoria, and Myrothecium diseases
Lime sulfurcontrols powdery mildews, anthracnose, and scab
Mancozeba very efficient protectant fungicide, controls early and late
blights of potato and tomato, downy mildews of grapevine, hops, cucurbits, lettuce, tobacco, and leeks, powdery mildews and rusts, scab of
apple and pear, anthracnose of beans and cucurbits, and Cercospora
leaf spots of banana, peanut, sugar beet, berries, and currants; controls
Botrytis, Cochliobolus, Colletotrichum, Corticium, Diaporthe,
Drechslera, Fusarium, Gibberella, Glomerella, Magnaporthe,
Mycosphaerella, Pestalotiopsis, Phoma, Phytophthora, Alternaria,
Helminthosporium, Rhizoctonia, Pythium, and Sclerotinia diseases
Manebcontrols early and late blights of potato and tomato, rusts on cereals, roses, asparagus, beans, carnations, plums, and currants; downy
mildews of vines, onion, tobacco, hops, and ornamentals; scab of apples and pears, leaf spot diseases of beet, celery, and currants;
anthracnose diseases of beans and tulips; needle cast in forestry
Mepanipyrimcontrols Botrytis cinerea
Mepronilcontrols rice sheath blight, black scurf of potato, damping-off
diseases, Typhula snow blight of wheat, barley, and oats, and rust diseases
Mercuric oxideused as a wound protectant for pruning cuts and other
bark injuries on fruit trees and ornamental trees
Mercurous chlorideas a soil application it controls club root of brassicas and white rot in onion; controls dollar spot and Fusarium patch
on turf
Metalaxylcontrols fungi belonging to Peronosporales; Phytophthora,
Plasmopara, Peronospora, Pseudoperonospora, Pythium, and
Sclerospora diseases; used as a seed treatment in many crops
Metalaxyl-Mone of the two enantiomers of metalaxyl; controls Pythium

and Phytophthora diseases
Metamcontrols Rhizoctonia solani, Armillaria mellea, and Sclerotinia
sclerotiorum
Metconazolecontrols Rhizoctonia solani, Mycosphaerella graminicola,
Fusarium culmorum, Septoria tritici, Rhynchosporium secalis, and
Leptosphaeria nodorum in cereals; also controls powdery mildews and
rusts
Metham-sodiumused as a soil fumigant; controls soil fungal pathogens
such as Verticillium, Fusarium, and Rhizoctonia
Methasulfocarbapplied as a dust into nursery soil before sowing; controls rice seedling blight caused by Pythium, Fusarium, Rhizoctonia,
Rhizopus, and Mucor
Methoxyethylmercury acetateuseful as a seed dressing for controlling
seed-borne diseases of wheat, barley, oats, and rye
2-Methoxyethylmercury chloridecontrols Ustilago scitaminea,
Rhizoctonia solani, and Fusarium
Methyl bromidea fumigant; soil fumigation controls soilborne diseases;
Fusarium, Armillaria, Rhizoctonia, Sclerotinia, and Pythium diseases
Methyl isothiocyanatesoil fumigant for control of soil fungal pathogens
Methyl jasmonateinduces systemic resistance against pathogens; controls Botrytis cinerea
Metiramcontrols scab of apple and pear, rust on asparagus, currants,
and plums, late blight of potato and tomato, downy mildews on vines,
hops, tobacco, and lettuce; also controls Alternaria, Botrytis,
Rhizoctonia, and Leptosphaeria diseases; as a seed treatment controls
pre- and postemergence diseases of vegetables
Metominostrobincontrols rice blast
Monoxcontrols Pythium aphanidermatum
Myclobutanilcontrols rusts and powdery mildews; Diplocarpon rosae
and Sphaerotheca pannosa in rose; Pythium, Rhizoctonia, Monilinia,
and Thielaviopsis basicola diseases; used as a seed treatment for control of seed- and soilborne diseases in wheat, rice, barley, corn, and
cotton; controls postharvest diseases
Nabamfungicide with protective action; controls soilborne diseases in
cotton, capsicums, and onion when applied to soil
Nimbicidinecontrols Erysiphe polygoni
Nitrothal-isopropylused in combination with other fungicides for control of powdery mildews and scab of pome fruits
Norspermidinecontrols Erysiphe graminis
Nuarimolcontrols powdery mildews in pome fruits, stone fruits, vines,
hops, cucurbits, and cereals; apple scab, smuts; Cercosporella and
Septoria diseases of cereals; used both as a foliar spray and as a seed

treatment
Octhilinonehas both fungicidal and bactericidal action; controls apple
and pear canker (Nectria galligena), Phytophthora, and Ceratocystis
diseases of citrus; citrus bacterial canker
Ofuracecommonly used in combination with other fungicides such as
mancozeb, folpet, captafol, zineb, and maneb; controls downy mildews
and Phytophthora diseases; dead arm (Phomopsis viticola) and black
rot (Guignardia bidwellii) of grapevine
Oxadixylin combination with contact fungicides it controls downy mildews; controls Alternaria, Fusarium, Gibberella, Phoma, and Phytophthora diseases
Oxine-copperas a seed treatment it controls bunt, glume blotch, and
snow mold of wheat; controls Cercospora, Alternaria, Phoma,
Pythium, Botrytis, and Sclerotinia diseases; also controls scab and canker diseases in apple and pears
Oxycarboxincontrols rust diseases, fairy rings in turf, and coffee rust
Paclobutrazolcontrols Diplodia and Rhizoctonia diseases
Penconazolehighly effective against powdery mildews (Erysiphe,
Oidium, Pseudoperonospora)
Pencycuronnonsystemic fungicide with specific action against
Rhizoctonia solani diseases such as rice sheath blight and black scurf
of potato
Perfurazoateas seed treatment controls blast, brown spot, and Fusarium
moniliforme
Phenylmercury acetateused as dry seed dressing; controls Gibberella
fujikuroi
2-Phenylphenoluseful for the control of storage diseases of apples,
plums, peaches, citrus fruits, tomato, and capsicums by impregnation
of fruit wrappers, crates, etc., or by application as a wax directly to the
fruit; used for disinfection of seed boxes; controls apple canker by application during the dormant period
Phosdiphencontrols rice blast
Pimaricincontrols diseases of bulbs, especially basal rot of daffodils
Polyoxinsantibiotics; control vegetable diseases
Primisulfuroncontrols Gibberella zeae
Prochlorazbroad spectrum fungicide; controls rusts and powdery mildews; Fusarium, Gibberella, Botrytis, Helminthosporium, Leptosphaeria, Mycosphaerella, Phoma, Pyrenophora, Colletotrichum,
Alternaria, and Pleospora betae diseases; also controls Pyrenophora
tritici, Pseudocercosporella herpotrichoide, and Erysiphe graminis in
wheat; peach leaf curl and rice blast, bakanae, and Helminthosporium
diseases; as a liquid seed treatment controls seedborne diseases of linseed and flax
Procymidonecontrols Botrytis, Sclerotinia, Fusarium, Alternaria,
Diaporthe, Glomerella, Phomopsis, Monilinia, Cladosporium
cucumerinum, and Stemphylium diseases
Propamocarbsystemic fungicide effective against oomycete fungi such
as Pythium, Phytophthora, and downy mildew fungi
Propiconazolecontrols brown rot, blossom blight, and fruit rot in stone
fruits; powdery mildews, bunts, and rusts; also controls Armillaria,
Cercospora, Cochliobolus, Colletotrichum, Glomerella,
Gaeumannomyces, Helminthosporium, Monilinia, Mycosphaerella,
Phaeoisariopsis, Phomopsis, Pleospora, Pseudocercosporella,
Rhizoctonia, Rhynchosporium, Septoria, Setosphaeria, Stagonospora,
and Pyrenophora diseases
Propinebcontrols downy mildews, early and late blights of potato and
tomato, scab on apple and pear; Glomerella, Cercospora, and
Rhizoctonia diseases
Pyrazophoscontrols powdery mildews, black Sigatoka of bananas, and
leaf spot diseases in cereals
Pyrifenoxsystemic fungicide with protective and curative activity
against powdery mildews, scab of apple and pears, Monilia in top fruit,
Sigatoka in banana
Pyrimethanilcontrols Botrytis cinerea, Venturia inaequalis, and
Sclerotinia fuckeliana diseases
Pyroquiloncontrols rice blast
Quinomethionatecontrols powdery mildews of cucurbits, citrus, tobacco, and pome fruits
Quinosolcontrols Phytophthora diseases
Quinoxyfeneffective against powdery mildews
Quintozenea contact fungicide; controls dollar spot, red thread, and
snow mold in turf; controls Pythium, Rhizoctonia, Sclerotium,
Sclerotinia, Botrytis, Diaporthe, Phomopsis, Corticium, and
Botryodiplodia diseases; controls club root of cabbage, bunts of wheat,
potato black scurf, smut and white rot of onions, and potato bacterial
disease caused by Streptomyces scabies
Salicylic acidinduces resistance and reduces disease incidence; effective against fungal, bacterial, and viral diseases; controls fungal pathogens Magnaporthe grisea, Fusarium oxysporum, and Diplodia pinea,
bacterial pathogen Xanthomonas oryzae pv. oryzae, and the virus cucumber mosaic virus (cucumovirus)
Spiroxaminecontrols powdery mildews and rusts; Pyrenophora teres,

Rhynchosporium secalis, Septoria tritici, Fusarium culmorum, and
Leptosphaeria nodorum diseases in cereals
Strobilurincontrols Leptosphaeria nodorum, Mycosphaerella
graminicola, Podosphaeria, Septoria, and Venturia diseases
Sulfurcontrols powdery mildews; scab on apples, pears, and peaches;
controls Colletotrichum, Corticium, Elsinoe, Glomerella, and Taphrina
diseases
Tebuconazoleeffectively controls powdery mildews, bunts, scabs, and
rusts; diseases caused by Alternaria, Ascochyta, Botrytis, Cercospora,
Colletotrichum, Diplocarpon, Fusarium, Rhizoctonia, Monilinia,
Mycosphaerella, Leptosphaeria, Gibberella, Nectria, Monographella,
Monilinia, Phaeoisariopsis, Phomopsis, Phytophthora,
Rhynchosporium, Septoria, Sclerotinia, and Setosphaeria
Tecnazenecontrols dry rot in seed potatoes and ware potatoes; inhibits
sprouting; smoke formulations for control of Botrytis on tomato, chrysanthemum, and lettuce
Tetraconazolecontrols rusts; Cercospora and Cochliobolus diseases
Thiabendazolecontrols Fusarium, Gibberella, Mycosphaerella, Phoma,
Phomopsis, Aspergillus, Botrytis, Colletotrichum, Rhizoctonia,
Sclerotinia, Septoria, Thielaviopsis, Verticillium, and Diaporthe diseases; controls dollar spot and red thread in turf
Thiophanatecontrols scab of apple and pear, powdery mildews,
Cercospora leaf spots, Verticillium and Fusarium wilts; Botrytis,
Pyricularia, Rhizoctonia, and Sclerotinia diseases
Thiophanate-methyla systemic broad spectrum fungicide; controls apple and pear scab and powdery mildews; effective against Rhizoctonia
and Fusarium diseases; also controls Colletotrichum, Diplocarpan,
Gibberella, Glomerella, Phoma, Macrophomina, Leptosphaeria, and
Nectria diseases
Thiramused mostly as a seed treatment to control damping-off and
emergence diseases in various crops; controls scab and Gloeosporium
rot in apples and pears, peach leaf curl, and rusts on ornamentals; also
controls Botrytis, Monilia, Cochliobolus, Corticium, Diaporthe,
Fusarium, Gibberella, Glomerella, Leptosphaeria, Macrophomina,
Mycosphaerella, Phoma, Phomopsis, Rhizoctonia, Sclerotinia,
Stagonospora, and Thielaviopsis diseases
Tolclofos-methylspecifically useful in control of black scurf and stem
canker in potatoes; used as a seed, bulb, or tuber treatment, soil drench,
foliar spray, or by soil incorporation; controls soilborne diseases
caused by Fusarium, Rhizoctonia, Sclerotium, and Typhula; also controls Phoma, Helminthosporium, Corticium, and Gibberella diseases
Tolyfluanidcontrols scab of apples and pears, Botrytis on strawberries,

raspberries, blackberries, currants, and grapes; also controls Diaporthe,
Gibberella, and Phomopsis diseases; reduces powdery mildew incidence
Triadimefoncontrols powdery mildews in cereals, vines, pome fruit,
stone fruit, hops, and cucurbits; rusts in cereals, pines, coffee, and turf
grasses; Monilinia spp. in stone fruit, black rot of grapes, snow mold in
cereals
Triadimenolcontrols rusts, powdery mildews, and Rhynchosporium in
cereals; as a seed treatment controls smuts, bunts, Pyrenophora,
Cochliobolus, Mycosphaerella, and Typhula in cereals; also controls
Diaporthe, Fusarium, Phomopsis, Rhizoctonia, Pseudocercosporella,
and Thielaviopsis diseases
Trichlamidecontrols Aphanomyces root rot of peas and radishes; club
root of cabbage; potato scab (Streptomyces scabies)
Tricyclazolecontrols rice blast
Tridemorphcontrols powdery mildews and rusts; Sigatoka disease of
banana, blister blight of tea, pink disease of coffee and tea, and
Cercospora leaf spot of coffee; also controls Corticium, Cerotelium,
Colletotrichum, Glomerella, Mycosphaerella, and Pestalotiopsis diseases
Triflumizolecontrols Diplocarpon rosae and Sphaerotheca pannosa var.
rosae in rose; controls Corticium, Fusarium, Gibberella,
Helminthosporium, Phragmidium, and Rhizoctonia diseases
Triforinecontrols powdery mildews and rusts; controls turf diseases and
scab in apples and pears
Triticonazolecontrols rusts, powdery mildews, bunts, Rhynchosporium
secalis, Pyrenophora teres, and Cochliobolus sativus in cereals
Validamycin Acontrols rice sheath blight, black scurf of potato, and
web blight of forestry seedlings caused by Rhizoctonia solani
Vinclozolincontrols Botrytis diseases in various crops; also controls
Diplocarpon, Sphaerotheca, Diaporthe, Alternaria, Corticium,
Phomopsis, Leptosphaeria, Sclerotinia, Sclerotium, and Phragmidium
diseases
Zinc oxidecontrols Alternaria and Phytophthora diseases in potato
Zinebcontrols downy mildews, early and late blights in potato and tomato, anthracnose on beans, grapevine, and citrus fruits, scab on apples
and pears, and Cercospora leaf spots on bananas and many other crops;
also controls Botrytis diseases
Ziramcontrols Venturia, Monilia, Septoria, Cercospora, Colletotrichum,
Alternaria, and Taphrina diseases
FUNGICIDE APPLICATION
Fungicide Application Based on Computer-Based
Decision Support Systems
Several fungicides and ready-formulated mixtures of fungicides are
available. Even to control a single pathogen, several groups of fungicides
are available. Some fungicides should be applied through soil, others should
be applied through seed, and others should be applied on foliage to control
diseases. For example, metominostrobin has been developed as controlledrelease granules. The granules are applied to seedling boxes, and after two
days, the rice seedlings are transplanted in the field. The concentration of
metominostrobin in plants reaches its maximum within 24 hours. The activity of the fungicide persists even after 90 days and protects the plants against
the blast pathogen Magnaporthe grisea (almost throughout the crop period). There is almost no need to apply more fungicide after a one-time application of the granule (Tashima et al., 1999). Some fungicides, such as
Ferrax (flutriafol+ethirimol+thiabendazole) and Ridomil MZ (metalaxyl+
mancozeb), when applied as seed treatment protect the seedlings against
airborne infection by pathogens for at least a few weeks. Recently, some
long-term systemic protectants such as quinoxyfen and Bion have been developed. These long-lasting-protection fungicides are few in number and
they have premium prices. Very few fungicides offer protection for more
than two to three weeks. Hence, farmers must apply fungicides several
times to manage diseases. Application of several rounds of fungicides as
routine prophylactic sprays, even in the absence of disease, makes crop cultivation uneconomical. It is advisable to apply fungicides only when they are
needed. Unnecessary sprays should be avoided, and by this, the farmer can
save money.
A number of computer-based decision systems are available worldwide
for use by farmers to decide the precise application date and the fungicide to
be sprayed in the most effective way (Gleason et al., 1995). In Europe, the
computer-based decision system EPIPRE (EPIdemiology, PREdiction, and
PREvention) developed in the Netherlands is used to manage diseases of
wheat crops (Zadoks, 1981). In Germany, the decision support system
PRO PLANT is widely used. It predicts diseases in wheat, barley, rye,
triticale, sugarbeet, and potatoes and predicts application date and the fungicide to be sprayed (Frahm and Volk, 1994). In the United States, the system
MoreCrop (Managerial Option of Reasonable Economic Control of Rusts
and Other Pathogens) is used to manage wheat diseases (Cu and Line,
1994). Another system, WDCA (Wheat Disease Control Advisory), is used
in Israel. An American predictive model, MARYBLYT, is used to predict

the fireblight disease outbreak in apples (Lightner and Steiner, 1990). Firework, a fire blight predictive program, is used in New Zealand for management decisions in apple crops (Gouk et al., 1999). Other available computer-based decision support systems include DESSAC (Decision Support
System for Arable Crops) and Counsellor to manage diseases of various
crops, AU-Pnuts (Auburn University Peanuts) Advisory I and AUPNUT
systems to manage peanut diseases, FAST (Forecasting Alternaria solani
on Tomatoes), CU-FAST (Cornell University-FAST), and TOM-CAST
(Tomato Forcaster) systems to manage tomato diseases, and the BLITECAST (Blight Forecaster) system to manage potato late blight. (For further
details, see Chapter 16, Forecasting Models.)
Although many decision support systems are available, they are still not
very popular among farmers, which may be due to the high cost involved in
purchasing such a system. Furthermore, they involve additional work for the
farmers. The field must be regularly monitored for disease incidence, probably more than six times during the crop period. In some systems, farmers are
asked to assess disease incidence in 40 to 100 tillers or more than 100 leaves
selected at random across the field by counting lesions or pustules in each
sample. In other systems, weather factors must be monitored regularly; even
leaf surface wetness period must be recorded. The extra workload makes
many farmers hesitant to follow a computer-based decision support system.
Instead of a personal computer system, messages can also be communicated through a postal system to interact with growers. This system works
slowly, so a network system is often introduced instead. Interactive advisory
systems, in which observations by farmers are sent in, the risks analyzed
centrally, and recommendations are sent out, may help farmers to make decisions. Government institutions may provide a major advice service to the
farmers. For example, Kansas State University conducts a plant disease survey regularly and gives the names of the fields surveyed and the disease severity in those fields. These reports are prepared every week. These data are
transmitted through a computer-based electronic communication system.
The data can be used by growers in making fungicide application decisions.
In the United Kingdom, Agricultural Development and Advisory Service
(ADAS) issues leaflets on the use of fungicides to help farmers. These data
may also assist extension specialists in conveying the message to growers to
make the decision to spray fungicides (Sim et al., 1988). Spray warnings by
mail, radio, or answering service will also help farmers to make decisions to
spray fungicides.
Fungicide Application Based on Disease Threshold

Need-based application of fungicides would be ideal, although it is difficult to practice. In this method, fungicides are applied only when the disease
threshold level is reached in the field. Studies on determining the disease
threshold level of each pathogen are important. Threshold values establish
those population densities which, when exceeded, lead to commercial (economic) loss. This threshold defines the optimal time for applying fungicide.
The effectiveness of fungicide treatments depends on the flexibility and
adaptability of pathogen-specific threshold values, as well as on cultural
and environmental influences. Specific epidemiological control thresholds
for the most important pathogens of wheat (Stagonospora nodorum, Septoria
tritici, Puccinia striiformis, Erysiphe graminis f. sp. tritici, Pseudocercosporella herpotrichoides, and Drechslera tritici-repentis) have been developed through various field trials (Verreet et al., 2000). For example, the disease threshold level of Erysiphe graminis was calculated as follows: At
weekly intervals, disease incidence was calculated from a sample of 30
plants. The first threshold of 70 percent disease incidence is reached if mildew pustules have been diagnosed on 21 of 30 plants in the sample. The second threshold of 70 percent disease incidence is reached if, on the topmost
green leaves, an average of 21 of 30 plants show at least one powdery mildew pustule. The fungicide is sprayed whenever the threshold level is
reached (Verreet et al., 2000). The use of fungicide should be avoided when
the threshold value is not exceeded. This fungicide application strategy will
be highly economical and useful in the control of diseases. However, this
method is highly knowledge intensive, and many farmers may not be able to
follow it. In fact, according to a published report, many farmers in the
United Kingdom were not able to correctly diagnose disease symptoms in
wheat. Only 13 percent of farmers could correctly recognize early yellow
rust symptoms, and only 24 percent of farmers could recognize glume
blotch (Hewitt, 1998).
Routine Prophylactic Fungicide Application
Prophylactic application involves routine preventative fungicide applications at set intervals. There is no monitoring of disease, and fungicides may
be applied irrespective of disease levels. This method is very simple, and
there is no need for planning. It would be ideal in crops in which a particular
disease occurs regularly during the particular season. For example, late
blight occurs regularly in potato, and five or more routine rounds of prophylactic sprays will provide effective control of the disease. When a greater
number of diseases occur in a crop, ready-formulated mixtures of fungicides can be used. Fungicides with long-lasting protection will be ideal for
this method of application. However, these fungicides are normally more
expensive.The major disadvantage of this method is that fungicides may be
used even when there is little or no risk of a disease outbreak, so money may
be wasted. For example, weekly sprays of captan or thiram starting from
early in the season did not reduce the incidence of fruit rot caused by Botrytis cinerea in strawberry until at least the fourth week of harvest, nine to ten
weeks after application began. Bloom applications of iprodione applied
twice during the second peak flowering period controlled the disease effectively. However, bloom application of iprodione during the first flowering
period did not control the disease effectively. This suggests application of
fungicides at the correct stage of the crop is important in disease control
(Legard et al., 2001). Another drawback is that an unexpected disease epidemic may occur between sprays when the crop is effectively left unsprayed.
FUNGICIDE RESISTANCE
What Is Fungicide Resistance?
After introduction of systemic fungicides, fungicide resistance has been
observed in various plant pathogens. Fungi that are sensitive to a particular
fungicide becomes less sensitive to that fungicide, which is called fungicide
resistance. Fungicide resistance has been reported in Phytophthora infestans, Peronospora parasitica, and Bremia lactucae against phenylamides
(metalaxyl compounds); Ustilago nuda and Ustilago maydis against carboxamides (carboxin compounds); Erysiphe graminis f. sp. hordei against
hydroxypyrimidine; E. graminis f. sp. tritici, E. graminis f. sp. hordei, and
Pyrenophora teres against triazoles (propiconazole); and Botrytis cinerea,
Botrytis fabae, Septoria tritici, Pyrenopeziza brassicae, Cercospora beticola,
Fusarium nivale, Fusarium culmorum, and Pseudocercosporella herpotrichoides against benzimidazoles (carbendazim, benomyl, thiophanate-methyl
compounds). There are only a few reports of resistance to surface-protectant
fungicides such as copper fungicides, organomercurials, organotin, and dithiocarbamates.
Fungi may become resistant to fungicides through several mechanisms.
The fungicide may act at a particular site in fungi. A change at the site of inhibitor action may result in a decreased affinity to the fungicide. Decreased
uptake or decreased accumulation of the fungicide in the fungus may result
in fungicide resistance. Sometimes increased production of an enzyme that
is normally inhibited by the fungicide may compensate for the inhibitory ef-
fect of the fungicide. The fungicide may also be detoxified before it reaches
the site of action, resulting in fungicide resistance in fungi. The fungicide
will be normally converted into a toxic principle to inhibit the fungi. However, at certain conditions, the fungicide would not have been converted into
the toxic principle, and this will result in fungicide resistance in fungi. Fungicides may block a site in an important metabolic pathway in fungi. When
circumvention of the blocked site by the operation of an alternative pathway
occurs, fungicide resistance can be observed in fungi.
How Fungicide-Resistant Pathogens
Develop in the Field
Fungicide-resistant isolates may arise by mutation of a single gene. Systemic fungicides usually act at a single site, so resistance to systemic fungicides occurs frequently. It is unlikely that two or more mutations will occur
simultaneously in fungi so that the fungi become resistant to multisite fungicides (mostly protectants). The fungicide-resistant cells may appear spontaneously in the field, normally at the rate of 1 in 104 to 109, even when no
fungicides are applied in the field. When the fungicide is applied to the crop,
all but resistant strains of the pathogen may be killed. This will allow the resistant strains to multiply. However, it has been demonstrated that the resistant strains do not survive for a long time. By the time the effects of the fungicide diminish, probably within a fortnight, the sensitive (wild) strains
reappear as the dominant partners in the pathogen population. However, resistant strains may continue to predominate if the same fungicide is repeatedly applied at short intervals. During the constant presence of the fungicide, the sensitive isolates may not have time to reestablish. Resistant strains
may multiply faster than the sensitive wild isolates which may result in a
condition in which control of the disease with the particular fungicide is no
longer effective in the field.
Development of Cross-Resistance in Fungal Pathogens
When a fungal pathogen develops resistance to a fungicide with a particular mode of action, it may show resistance to other fungicides with the
same mode of action. This phenomenon is called cross-resistance. The
phenylamide fungicides metalaxyl, benalaxyl, ofurace, and oxadixyl show
a similar mode of action against fungi. The isolates of Phytophthora
infestans resistant to metalaxyl show resistance to other phenlamides,
benalaxyl, ofurace, and oxadixyl. Sometimes resistance to one fungicide
may result in increased sensitivity to another fungicide. This is called nega-
tive cross-resistance. Blumeria graminis isolates, which show resistance to

triazoles, show increased sensitivity to ethirimol compared to the wild isolates without any resistance to the fungicides.
Management of Fungicide Resistance in Fungal Pathogens
The following strategies are suggested to avoid development of fungicide
resistance in fungal pathogens:
1. Consecutive applications of fungicides with the same mode of action
should be avoided.
2. Seed treatments with systemic fungicides should not be followed by
foliar sprays of fungicides with the same mode of action.
3. The spray schedule should include some multisite fungicides.
4. Fungicides should be sprayed only when necessary. Fungicides should
not be sprayed when there is little disease. Very early and late applications should be avoided as much as possible. Prophylactic sprays
should be avoided; if this is not possible, prophylactic sprays should
be restricted to three sprays.
5. Instead of spraying a fungicide with a single active ingredient, a fungicide with a mixture of active ingredients should be used. The risk of
the development of resistant strains is significantly reduced if active
ingredients with two modes of action are mixed. Farmers themselves
can mix fungicides using the compatibility chart provided by the manufacturers. However, many pesticide-manufacturing companies now
produce ready-formulated mixtures. These mixtures consist of sitespecific fungicides with different modes of action or multisite fungicides.
REFERENCES
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Ellis, M. C. B. and Tuck, C. R. (2000). The variation in characteristics of airincluded sprays with adjuvants. Aspects of Applied Biology, 57:155-162.
Frahm, J. and Volk, T. (1994). PRO-PLANTA computer-based decision support
system for cereal disease control. EPPO Bulletin, 23:685-694.
Gleason, M. L., MacNab, A. A., Pitblado, R. E., Ricker, M. D., East, M. D., and
Latin, R. X. (1995). Disease-warning systems for processing tomatoes in Eastern
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Gouk, S. C., Spink, M., and Laurenson, M. R. (1999). FireWorkA windowsbased computer program for prediction of fire blight on apples. Acta Horticulturae, 489:407-412.
Grayson, B. T., Webb, J. D., Batten, D. M., and Edwards, D. (1996). Effects of adjuvants on the therapeutic activity of dimethomorph controlling vine downy mildew. I. A survey of adjuvant types. Pesticide Science, 46:199-206.
Hewitt, H. G. (1998). Fungicides in Crop Protection. CAB International, University Press, Cambridge, U.K.
Knight, S. C., Anthony, V. M., Brady, A. M., Greenland, A. J., Heaney, S. P.,
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Kwon, Y. W., Lee, J. K., and Chung, B. J. (1989). Interaction of adjuvants with rice
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Legard, D. E., Xiao, C. L., Mertely, J. C., and Chandler, C. K. (2001). Management
of Botrytis fruit rot in annual winter strawberry using captan, thiram, and iprodione. Plant Dis, 85:31-39.
Lightner, G. W. and Steiner, P. W. (1990). Computerization of blossom blight prediction model. Acta Horticulturae, 273:159-162.
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Ragsdale, N. N. and Sisler, H. D. (1991). The nature, modes of action, and toxicity
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24
Chemical ControlFungicide
Chemical Control
Application Equipment
Fungicide Application Equipment
Application equipment is needed to get the fungicide to the target crop. If

proper application equipment is not used, even the most efficient fungicide
will not be able to control the disease. Fungicides are available as dustable
powder, wettable powder, liquids, emulsifiable concentrate, oil formulations,
ultralow-volume formulations, and gases. Fungicides need to be applied in
dwarf crops such as peanut as well as in large-sized tall trees such as apples.
Hence, different types of application equipment are needed to apply different formulations of fungicides to different types of crops. This chapter describes all available application equipment.
DUSTERS
Hand Dusters
These dusters are primarily used by home gardeners. They may consist
of a squeeze bulb, bellows, tube, sliding tube, shaker, or a fan powered by a
hand crank. When the dusters are used it will be difficult to control drifting,
causing wastage of fungicides. However, they are easy to operate. Because
no mixing with water is needed, the user does not need to make calculations
and prepare solutions.
Power Dusters
Power dusters contain a powered fan or blower to propel the fungicide
dust to the target. Several types of power dusters are available. Knapsack
types, backpack types, and units mounted on or pulled by tractors are available.
SPRAYERS
Push-Pull Hand Sprayer
Hand sprayers operate on compressed air, which is supplied by a manually operated pump. They are primarily used in home gardens. Push-pull
hand sprayers contain a hand-operated plunger which forces air out of a cylinder, creating a vacuum at the top of a siphon tube. The suction draws fungicide from the small tank and forces it out with the airflow. The capacity is
approximately one quart.
Hose-End Hand Sprayer
In this type of sprayer, a fixed rate of fungicide is mixed with water flowing through the hose to which the sprayer is attached. The mixture is expelled through a high-volume nozzle. The capacity of these sprayers is approximately one quart, but because the concentrate mixes with water, they
may deliver about 20 gallons of finished spray solution.
Trigger-Pump Sprayer
The fungicide is mixed with the required quantity of water in the sprayer
and forced through the nozzle when the pump is squeezed. Trigger-pump
sprayers are useful for applying only small quantities of fungicide and are
intended for small jobs.
Backpack Sprayer
This sprayer is similar to a push-pull sprayer. However, it contains a selfcontained tank and a pump. A mechanical agitator is attached to the pump
plunger. The sprayer is carried on the operators back.
Compressed Air Sprayer
This is a hand-carried sprayer that contains a self-contained manual
pump, which creates pressure and operates the sprayer. The capacity of the
sprayer is approximately one to three gallons.
Bucket (or Trombone) Sprayer
This sprayer contains a double-action hydraulic pump, which is operated
with a push-pull motion. The fungicide is sucked into the cylinder and pushed
out through the hose and nozzle with a stroke. The separate tank is a small
bucket.
Estate Sprayer
This sprayer is mounted on a two-wheeled cart with handles for pushing.
The tank capacity ranges from 15 to 50 gallons. The pump delivers one to
four gallons per minute at pressures ranging from 250 to 400 psi. Power is
supplied by an air-cooled engine of up to five horsepower.
Power Wheelbarrow Sprayer
This is similar to estate sprayer, but it is driven by power wheel.
Low-Pressure Boom Sprayer
This type of sprayer is mounted on tractors or trucks. Tank size ranges
from 50 gallons to 1,000 gallons. The sprayer delivers ten to 60 gallons per
acre at pressures ranging from ten to 80 psi. The sprayer is equipped with
sprayer booms ranging from ten to 60 feet in length. The booms contain
many nozzles.
Low-Pressure Boomless Sprayer
This sprayer does not contain the boom. It contains a central nozzle cluster that produces a horizontal spray pattern. This sprayer can move through
narrow places and avoid trees and other obstacles, unlike the boom sprayer.
High-Pressure Hydraulic Sprayer
This sprayer is useful to spray through dense foliage, to the tops of tall
trees. The sprayer is mounted on a tractor or truck or is self-propelled. It delivers about 60 gallons per minute, and the application rate is about 100 gallons per acre. The sprayer contains a large tank with a capacity of 500 to
1,000 gallons to cover large areas. The sprayer is also fitted with a boom.
Air-Jet (Air-Blast) Sprayer
This sprayer uses a combination of air and liquid. It is similar to highpressure sprayers but also contains a high-speed fan. The air jet shatters the
drops of fungicide into fine droplets and transports them to the target. This
sprayer is usually trailer mounted. An air-jet sprayer covers a swath up to 90

feet wide and reaches trees up to 70 feet tall. A device that permits precision
spraying in orchards with an air-blast sprayer has been developed (Balsari
and Tamagnone, 1997). A computer commands the appropriate valves,
which control nozzle output according to ground speed, row spacing, and
tree shape. Only the necessary number of nozzles is turned on to cover a particular size.
Tunnel Sprayer
A tunnel sprayer is a type of air-jet sprayer. Two types of tunnel sprayers
have been developed. The ISK-2 tunnel sprayer contains eight air jets which
are directed 40 upward. The OSG-NI tunnel sprayer contains two horizontal jets blown by cross-flow fans (Holownicki et al., 1997). The best distribution of spray deposits on leaf surfaces is obtained with the ISK-2 tunnel
sprayer. The best practical method for economical fungicide application in
orchards is the tunnel sprayer with sensors. The spray-liquid recirculation
system in tunnel sprayers reduces fungicide consumption by 20 to 40 percent (Holownicki et al., 1998). Sensors identify the objects to be sprayed.
Spinning Disc Sprayer
This sprayer contains a special type of nozzle, which spins at a high
speed and breaks the liquid into uniformly sized droplets by centrifugal
force. A small electric or hydraulic motor provides the power to spin.
Fungicide droplets are carried to the target crop canopy by gravity or by an
airstream. Different sizes of the sprayers are available. Sizes range from a
small hand-held sprayer to a large tractor- or trailer-mounted sprayer. The
sprayer produces a narrower range of droplet sizes, thus reducing drift.
Electrostatic Sprayer
The fungicide is charged with a positive electric charge as it leaves the
nozzles. Plants have a natural negative charge. Hence, the fungicide is attracted to the plants. The spray is directed through or above the crop. The
electrostatic sprayer system reduces drift. Because the fungicide is directed
only to the crop plants, a reduced quantity of the fungicide is sufficient to
cover an acre.
Ultralow-Volume Sprayer
This sprayer sprays specially prepared concentrated fungicides. It can be
handheld or mounted on ground equipment or on aircraft.
GRANULE APPLICATORS
Some fungicides are available in granular form. Several types of granule
applicators have been developed. They have been designed to apply granules as broadcast with even distribution over the entire area, to apply them
by drilling (soil injection or soil incorporation), or to apply them in furrows,
in bands, or as side dress. They distribute the granules by forced air, by spinning or whirling discs, or by soil injectors.
SEED TREATERS
Seed treaters are used to coat seeds with seed-treatment fungicide. Four
types of seed treaters are available.
Dust Treater
Seed is treated with a dust formulation of the fungicide in this treater. It
mixes fungicide with seeds in a mechanical mixing chamber. Seed flow is
controlled by adjusting the gate opening on the feed hopper. The amount of
fungicide added is controlled by a vibrating feeder, which can be adjusted to
achieve the desired dosage.
Slurry Treater
This treater is used to coat seeds with wettable powder formulations of
fungicides in the form of a slurry. A specific amount of fungicide is added to
a specific weight of seeds in a mechanical mixing chamber. A small amount
of water is added. Agitators keep the material mixed during the treating operation. The capacity of slurry tanks ranges from 15 to 35 gallons.
Panogen Liquid Treater
A small amount of liquid fungicide is added to a large quantity of seeds
and mixed well in this treater. Some treaters may have dual tanks so that
seeds can be treated with more than one fungicide at a time. The Panogen
treater meters one treatment cup of fungicide per dump of seed into a revolving mixing drum. The fungicide flows into the drum from a tube and is
distributed over the seeds as the seeds rub against the walls of the drum.
Mist-O-Matic Seed Treater

Fungicide is applied as a mist onto the seed in this seed treater. The
treater delivers one treatment cup of fungicide per dump of seed. The fungicide flows onto a rapidly whirling disc, which breaks the liquid fungicide
into a fine mist. The seeds fall onto a large cone, which spreads the seeds out
so that they are evenly coated with the fungicide spray mist.
SOIL APPLICATION EQUIPMENT
Soil Fumigators
Some fungicides are available in gaseous form. They are applied in soil
to control soil-borne pathogens. These fungicides are applied to soil by different methods, such as soil injection, soil incorporation, or drenching, using different types of applicators.
Soil Injectors
Soil injectors use a variety of mechanisms to insert the fungicide into the
soil to a depth of about six inches. After injection, the area is covered with
soil to seal in the applied fungicide.
Soil Incorporators
The fumigant fungicide is sprayed onto the soil surface by using soil incorporators. The applied area is immediately plowed to a depth of about five
inches using power-driven rotary cultivator and then compacted with a drag,
or float, or cultipacker.
OTHER TYPES OF EQUIPMENT
Fruit Tree Injector
A portable high-pressure fruit tree injector has been developed. It injects
liquid fungicides into the trunk of fruit trees to control fruit-tree diseases.
The injector can be used even for large trunks (Liu et al., 1998).
Drenching Equipment
The fungicide is added to water and applied as a drench. It may be applied using a sprinkling can, sprinkler system, or irrigation equipment. The
fumigant fungicide can also be sprayed on the soil surface, immediately followed by flooding. Here the water acts as a sealant, and the depth of the water seal ranges from one to four inches of wetted soil.
Pressure-Fed Applicator
This applicator is useful to apply liquid fumigant fungicide. The applicator has a pump and metering device and delivers pressured liquid fumigant
to the nozzle opening (orifice).
Gravity-Fed Applicator
In this applicator, the pressure is created by gravity to regulate the output
of the liquid fumigant. A constant gravity flow device keeps the pressure at
the orifice constant as the tank of fumigant empties. A constant speed is necessary to maintain a uniform delivery rate. Needle valves, orifice discs, and
capillary tubes are used to adjust the flow rate.
High-Pressure Fumigator
This applicator is used to apply highly volatile fumigants. The pressure
in the tank maintains the pressure at the nozzle orifices. The tank is precharged with sufficient pressure to empty its contents, or an inert pressurized gas is fed into the tank during application to displace the fumigant. A
gas-pressure regulator maintains uniform pressure in the system. After application of a highly volatile fumigant, the soil should be sealed with vaporproof tarps.
PARTS OF APPLICATION EQUIPMENT

Components of application equipment determine the efficacy of fungicide application. The important components of the sprayers are the tank,
pump, strainer, hose, pressure gauge, pressure regulator, agitator, control
valve, and nozzle. The tank should be made of a corrosion-resistant material. Different types of pumps are used, including roller pumps, gear pumps,
diaphragm pumps, piston pumps, and centrifugal pumps. Roller pumps de-
liver eight to 30 gallons per minute at low to moderate pressure (10 to 300
psi). They are often used on low-pressure sprayers. Gear pumps deliver 5 to
65 gpm at low to moderate pressures (20 to 100 psi). They are often used on
special-purpose sprayers. Diaphragm pumps deliver low volume (3 to 10
gpm) at low to moderate pressure (10 to 100 psi). Diaphragm pumps are
positive-displacement, self-priming pumps. Centrifugal pumps deliver high
volume (about 200 psi) at low pressures (5 to 70 psi) and are not positivedisplacement pumps. Piston pumps are used in high-pressure pumps. They
are positive-displacement, self-priming pumps, delivering two to 60 gallons
per minute at low to high pressures (20 to 800 psi).
Strainers filter the fungicide mixtures and remove dirt, rust flakes, and
other foreign materials from the tank mixture. Proper filtering helps to protect the working parts of the sprayer from undue wear and avoids uneven application caused by clogged nozzle tips. Hoses should have a burst strength
greater than the peak operating pressures. They should resist oil and solvents present in fungicides. Suction hoses should be larger than pressure
hoses. Suction hoses should be reinforced to resist collapse. Pressure gauges
monitor the function of the spraying system and must be accurate. The pressure regulator controls the pressure and the quantity of spray material delivered by the nozzles. Three types of pressure regulators are available. Throttling valves are used with centrifugal pumps. Spring-loaded bypass valves
are used with diaphragm, roller, gear, and small piston pumps. They open or
close in response to changes in pressure. Unloader valves are used on larger
piston and diaphragm pumps to avoid damage to the pump when the nozzles
are cut off.
Agitators help to keep the spray material uniformly mixed. Several types
of agitators are used in the sprayers. Bypass agitators use the returning liquid from the pressure relief valve to agitate the tank. Bypass agitation is
used to agitate soluble powders, emulsifiable concentrates, and liquids.
They are not useful for wettable powders. Hydraulic agitators are used for
wettable powder and flowable formulations and for liquid formulations in
100-gallon or larger tanks with gear, piston, roller, or diaphragm pumps.
Mechanical agitators are used on large, high-pressure hydraulic sprayers.
They help to mix wettable powder formulations. The mechanical agitator
consists of flat propellers mounted on a shaft, which is placed lengthwise
along the bottom of the tank. The propellers are rotated by the engine to
keep the material well mixed.
Control (cutoff) valves are located between the pressure regulator and the
nozzles to provide positive on-off action. Mechanical or electrically operated valves are used. Mechanical valves are within reach of the operators
hand. Electrical operators permit remote control of fungicide flow.
Nozzles are very important for effective spraying of fungicides. They are
made up of the nozzle body, cap, strainer, and tip or orifice plate. The nozzle
body holds the strainer and tip in proper position. The cap is used to secure
the strainer and the tip to the body. Several types of tips that produce a variety of spray patterns can be interchanged. Nozzle tips provide the desired
type of spray. Solid-stream nozzles are used to spray in a narrow band or inject fungicides into the soil. Fan-pattern nozzles are used for spraying uniformly to cover surfaces. The regular flat-fan nozzle tip makes a narrow oval
pattern. The even net-fan nozzle is used for band spraying. The flooding
nozzle delivers wide-angle net-spray droplets. Cluster nozzles are used to
extend the effective swath width. Cone-pattern nozzles are used to apply
fungicides to foliage. The side-entry hollow-cone (whirl-chamber) nozzle
produces a very wide-angle hollow-cone spray pattern at very low pressures. Core-insert cone nozzles produce a solid or hollow-cone spray pattern
and operate at moderate pressures. Disc-core nozzles produce a cone-shaped
spray pattern, which may be solid or hollow. Adjustable-cone nozzles
change their spray angle from a wide cone pattern to a solid stream when the
nozzle collar is turned. Nozzle parts may be made of brass, aluminium,
stainless steel, tungsten carbide and ceramic, or plastic. Relative wear of the
nozzle orifice for different materials varied considerably with usage duration. Nozzles made of hardened stainless steel were most resistant to wear,
followed by stainless steel, plastic, and brass. Nozzles with lower flow capacities had a higher percentage of wear than nozzles with higher flow capacities (Duvnjak et al., 1998).
A novel air-delivery nozzle, called Shear-Guard PLUS nozzle, with DialA-Drop spray technology allows the applicator to precisely and instantly
dial in droplet size while on the go (McCracken et al., 1998).
REFERENCES
Balsari, P. and Tamagnone, M. (1997). An automatic spray control for airblast
sprayers: First results. In J. Stafford (Ed.), Precision Agriculture 97, Volume II.
Bios Scientific Publishers, Oxford, U.K., pp. 619-626.
Duvnjak, V., Banaj, D., Zimmer, R., and Guberac, V. (1998). Influence of nozzle
wear on flow rate and stream droplets size. Bodenkultur, 49:189-192.
Holownicki, R., Doruchowski, G., and Jaeken, P. (1998). Economical techniques of
orchard protection. Inzynieria Rolnicza, 5:269-278.
Holownicki, R., Doruchowski, G., and Swiechowski, W. (1997). Uniformity of
spray deposit within apple tree canopy as affected by direction of the air-jet in
tunnel sprayers. J Fruit and Ornamental Plant Research, 5:129-136.
Liu, D.L., Jian, X. C., Zhang, X. L., Zhang, L. X., and Zuo, B. Y. (1998). Application of a portable high pressure fruit tree injector. AMA, Agricultural Mechanization in Asia, Africa, and Latin America, 29(4):29-31.
McCracken, T., Bennett, A., and Jonasson, K. (1998). Improved spray efficacy and
drift management for spray application using air to simultaneously atomize and
propel spray droplets to the crop canopy. ASAE Annual International Meeting,
Orlando, Florida, U.S.A. American Society of Agricultural Engineers, St. Joseph, MO, p. 12.
25
Chemical
Chemical
ControlVirus
ControlVirus Diseases
Diseases
Chemical control of virus diseases is almost not practical. However, the
vectors of viruses can be controlled to a certain extent to reduce the disease
spread. The conditions necessary for vector management are discussed in
this chapter. Use of plant activators to manage virus diseases is also described.
CONTROL OF VIRUS SPREAD BY VIRICIDES
Chemical control of virus diseases is difficult to achieve. Only ribavirin
has been shown to reduce virus diseases. Ribavirin is effective against Potato S virus (Carlavirus) and Odontoglossum ringspot virus (Tobamovirus)
(Research Information Ltd., 1998). Virazole (ribavirin) at 25 mg/liter eliminated Apple chlorotic leaf spot virus (ACLSV) from treated apple and pear
shoots. However, ribavirin at a concentration of 100 mg was phytotoxic
(Cieslinska and Zawadzka, 1999).
CONTROL OF VIRUS SPREAD BY INSECTICIDES
Semipersistently and persistently transmitted viruses are acquired and
inoculated only after long feeding probes (generally hours to days). Plants
susceptible to infection often are colonized by the vector (Perring et al.,
1999). In these cases, spread of virus diseases can be efficiently controlled
by insecticides. For a nonpersistently transmitted virus, in which acquisition and inoculation can occur in a matter of seconds, the source and inoculation plants generally are not considered to be hosts by the vector. Hence, it
is difficult to expose the vector to a lethal dose of the insecticide prior to virus acquisition (Perring et al., 1999). The efficacy of insecticides on virus
disease spread varies depending on whether the virus is brought into a field
by insects already infective (primary spread) or whether the spread of virus
is from infected to healthy plants within a field (secondary spread). In the
case of primary spread, insecticide application to the crop may not be effec-
tive, especially if many viruliferous insects enter the field. If the virus involved is a nonpersistently transmitted virus vectored by a noncolonizing,
transient vector, insecticide application will be of no use. Virus control may
be possible by applying insecticides outside the field to the plants hosting
the vector, but this method may be impractical.
When the virus spread is secondary and the vector multiplies in the plant
as its host, application of insecticides may be effective in preventing the
spread of the virus. Systemic insecticides may be effective in the control of
persistently transmitted viruses. Persistently transmitted viruses often are
acquired from the phloem, so systemic insecticides in the phloem can kill
the vector prior to virus acquisition or inoculation. However, nonpersistently
transmitted viruses are acquired from and inoculated to epidermal cells, so
the insect does not contact the systemic insecticide (which accumulates in
phloem cells). Hence, it may not be possible to control nonpersistent viruses
with systemic insecticides. However, some chemicals kill the vector rapidly,
repel the vector, or modify vector behavior to prevent probing. Synthetic
pyrethroids cause rapid knockdown or mortality of vectors prior to virus inoculation. Some pyrethroids also repel insects. This action can cause increased aphid activity and enhance virus spread (Lowery and Boiteau,
1988). Pyrethroids reduce the probing time of vectors. It may reduce the disease spread because fewer insects acquire the virus when the plants are
treated with pyrethroids and viruliferous insects are prevented from inoculating healthy plants.
Mineral oils are known to reduce the spread of nonpersistent viruses
through reduction of vector densities. Oils may reduce feeding of the vector
and increase time spent by the vector on foliage before initiating probing.
Oils may impede the virus infection process, even though the virus is transmitted. Mineral oil does not block transmission, but symptom expression is
delayed in sprayed plants (Crane and Calpouzos, 1969).
Several newer insecticides have been reported to reduce virus spread,
including spread of nonpersistent viruses. Tomato yellow leaf curl virus
could be controlled by controlling its vector Bemisia tabaci by spraying an
imidacloprid insecticide, Confidor (Ahmed et al., 2001). The second generation neonicotinoid insecticide thiamethoxam prevents transmission of Tomato yellow leaf curl virus (TYLCV) by the whitefly B. tabaci (Mason et
al., 2000). The insecticide was applied as drench or foliar treatment. Drench
application to tomato plants provided a good level of protection from
TYLCV infections (from one to 22 days after treatment application); foliar
application resulted in a prompt but short-lasting protection ( eight days).
Foliar treatment of plants made infected tomatoes totally ineffective as a virus source for at least eight days. The percentage of viruliferous whiteflies
surviving the acquisition on the insecticide-treated plants appeared similar
to that of insects fed on untreated plants, suggesting that thiamethoxam activity in preventing TYLCV transmission by B. tabaci was simply due to its
killing activity, and not by antifeeding or repellent actions. Viruliferous
whiteflies exposed to thiamethoxam-treated plants stopped feeding before
acquiring enough virus to subsequently inoculate plants (Mason et al.,
2000). Deltamethrin + heptenophos (trade name Decisquick, marketed by
AgrEvo) effectively controls aphid vectors and prevents virus spread in potatoes, sugar beet, and peas. Aldicarb (Temik), aldicarb + gamma-HCH
(Sentry), carbosulfan (Marshal), demeton-S-methyl (Metasystox), Dimethoate (Dimethoate), disulfoton (Disulfoton), fenvalerate (Sumicidin), oxamyl
(Vydate), phorate (Phorate), pirimicarb (Aphox), deltamethrin (Decis),
cypermethrin (Ambush), and thimeton (Ekatin) have been reported to be effective against the virus diseases spread by the aphid vector Myzus persicae
(Parry, 1990).
CONTROL OF VIRUS DISEASES BY PLANT ACTIVATORS
Another method of controlling virus diseases by chemicals is by using
plant activators, which induce systemic resistance against virus infection.
Preplant application of the plant activator acibenzolar-S-methyl (Actigard)
effectively controls Tomato spotted wilt virus (TSWV) in tomato (Csinos
et al., 2001). Initial applications made posttransplant had no effect, suggesting that plants must be protected prior to introduction into the field. Therefore, the activator should be applied as preplant application to effectively
control the virus infection. For more information on plant activators, see
Chapter 28, Plant Activators.
REFERENCES
Ahmed, N. E., Kanan, H. O., Sugimoto, Y., Ma, Y. Q., and Inanaga, S. (2001). Effect of imidacloprid on incidence of Tomato yellow leaf curl virus. Plant Dis,
85:84-87.
Cieslinska, M. and Zawadzka, B. (1999). Preliminary results of investigation on
elimination of viruses from apple, pear, and raspberry using thermotherapy and
chemotherapy in vitro. Phytopathologia Polonica, 17:41-48.
Crane, G. L. and Calpouzos, L. (1969). Suppression of symptoms of sugarbeet virus
yellows by mineral oil. Phytopathology, 59:697-698.
Csinos, A. S., Pappu, H. R., McPherson, R. M., and Stephenson, M. G. (2001).
Management of Tomato spotted wilt virus in flue-cured tobacco with acibenzolar-S-methyl and imidacloprid. Plant Dis, 85:292-296.
Lowery, D. T. and Boiteau, G. (1988). Effects of five insecticides on the probing,

walking, and settling behavior of the green peach aphid and the buckthorn aphid
(Homoptera: Aphididae) on potato. J Econ Entomol, 81:208-214.
Mason, G., Rancati, M., and Bosco, D. (2000). The effect of thiamethoxam, a second generation neonicotinoid insecticide, in preventing transmission of tomato
yellow leaf curl geminivirus (TYLCV) by the whitefly Bemisia tabaci (Genadius). Crop Protection, 19:473-479.
Parry, D. W. (1990). Plant Pathology in Agriculture. Cambridge University Press,
Cambridge, U.K.
Perring, T. M., Gruenhagen, N. M., and Farrar, C. A. (1999). Management of plant
viral diseases through chemical control of insect vectors. Annu Rev Entomol,
44:457-481.
Research Information Ltd. (1998). International Pesticide Directory, Sixteenth Edition. Research Information Ltd., Herts, U.K.
26
Cultural
Cultural Methods
Methods
Crop diseases can be managed by manipulating some cultural practices.
The important useful cultural practices that help to reduce disease incidence
are described in this chapter. Sanitation is important to prevent the introduction of pathogen inoculum into fields, farms, or communities, and to reduce
or eliminate inoculum from diseased fields. Soil solarization and mulching
are novel approaches, which are now widely practiced in different parts of
the United States, Australia, and many other countries. Organic and inorganic soil amendments are increasingly used to manage diseases. Tillage
practices may be useful in reducing disease incidence in some crops.
Methods of sowing/planting, irrigation practices, and pruning methods also
determine disease incidence. Adjustment of the crop sequence providing a
fallow period and growing intercrops, living crop covers, and trap crops are
other useful approaches in crop-disease management.
PREVENTION OF INTRODUCTION OF INOCULUM
Pathogens may be introduced into a field, farm, or region by means of
seeds and vegetative propagating materials. Only seeds free from pathogens
should be used for planting. Methods of indexing seeds for the presence of
pathogens are described in Chapter 27, Exclusion and Eradication.
Debris from infected plants may carry the primary inoculum and may
serve as source for initial outbreak of diseases. Hence, removal of the debris
may be one of the most important cultural practices in management of crop
diseases. Venturia inaequalis survives the winter in diseased apple leaves on
the ground. Pseudothecia and ascospores develop in these fallen leaves.
Ascospores produced on diseased leaves in the leaf litter constitute the primary inoculum causing a scab. It has also been reported that viable conidia
of V. inaequalis can overwinter within apple buds (Becker et al., 1992). Because the primary inoculum for the disease arises from the fallen leaves,
crop-debris management may help in reducing disease outbreak. Infested
fallen leaves found on the orchard floor should be removed or destroyed to
reduce the overwintering inoculum. The degradation of fallen leaves can be
enhanced by spraying urea in the fall. The fallen leaves can be deeply incorporated into the orchard soil by tilling and by chopping leaves with flail
mowers (Sutton et al., 2000). Shredding the leaf litter in November or April
reduces the risk of scab by 80 to 90 percent in the northeastern United States
(Sutton et al., 2000). Urea applied to the leaf litter in April (before the bud
break) reduced the number of the fungal ascospores trapped by 66 percent
(Sutton et al., 2000). It appears that field sanitation, i.e., shredding the leaf
litter or treating the leaf litter with urea, is very important in managing apple
scab (MacHardy, 2000).
The citrus canker pathogen, Xanthomonas axonopodis pv. citri, survives
in infested leaf litters, and the bacterium can be recovered up to four months
after leaf fall. Soil treatments, including burial of leaves, reduce its survival
significantly. The bacterium can be recovered from soil beneath diseased
trees, but removal of the inoculum source leads to a demise of the bacterial
populations within days (Graham et al., 1989). The lettuce bacterial leaf
spot pathogen Xanthomonas campestris pv. vitians was recovered from lettuce plant debris after the one-month summer fallow and the disease developed on all subsequent fall lettuce crops (Barak et al., 2001). It stresses the
need for removal of crop debris to manage the disease.
Pruning of infected parts may reduce the disease outbreak in many orchard trees, because the primary inoculum is from infected plant tissues. In
fire blight-affected pome fruit trees, blighted blossom clusters and shoots
should be pruned. Infected parts should be cut back up to shoot basis and
branch ring. Once the disease has spread into the stem, the tree must be
eliminated (Richter, 1999). Pruning alone reduced the bacterial infection by
62 percent in pear (Aysan et al., 1999). It may be possible to preserve
Erwinia amylovora-attacked pear trees by pruning blighted blossom clusters and shoots. Leaf sanitation (removal of senescent and necrotic leaves)
reduced Botrytis rot incidence in strawberry from 13 to 8 percent (Mertely
et al., 2000).
Burning the infected organs may reduce the inoculum in the field. Pear
trees in commercial orchards with shoots or blossoms naturally infected
with Erwinia amylovora were burned for a few seconds with a propane
flame torch on the tissue surrounding infected sites (Reuveni et al., 1999).
Burning was highly efficient in eliminating E. amylovora in infected organs.
Burning also prevented the spread of the bacteria from the infected organs to
the main limb. No damage to tree viability and vitality was observed as a result of this treatment. Burning the infected organs before internal spread of
E. amylovora to the main limb occurs can provide a safe and rapid measure
to control fire blight in pear (Reuveni et al., 1999).
SOIL SOLARIZATION
Soil solarization, the process of heating soils under transparent plastic
tarps to temperatures detrimental to soilborne pathogens, has successfully
controlled a variety of plant diseases. Solarization targets mesophyllic organisms, which include most plant pathogens, without destroying the beneficial mycorrhizal fungi and growth-promoting Bacillus spp. Increased soil
temperatures result in decreased populations of a range of plant pathogens,
including fungi, bacteria, and nematodes. If not directly inactivated by heat,
soilborne plant pathogens may be weakened and become vulnerable to soil
fumigants, to other organisms, or to changes in the soil atmosphere in
solarized soil (Pinkerton et al., 2000). The solarized soils are often more
suppressive to certain soilborne pathogens than are nonsolarized soils. The
effectiveness of solarization against Verticillium wilt of safflower and cotton, Fusarium oxysporum f. sp. vasinfectum wilt of cotton, and several species of Phytophthora has been reported (Pinkerton et al., 2000). When the
soil was solarized for three weeks after the amendment with farmyard manure, the root rot of melon by Phomopsis sclerotioides was controlled to
some extent (Itoh et al., 2000). Soil solarization by covering the soil with a
150 m-thick transparent plastic film for two weeks brought about a high
degree of Pythium spp. control in greenhouse-grown cucumber (Lopes et
al., 2000). Solarization for three weeks using transparent polyethylene
sheets for soil mulching provided effective control of wilts caused by
Fusarium oxysporum f. sp. lycopersici and Verticillium dahliae, and corky
root rot caused by Pyrenochaeta lycopersici on tomato plants (Ioannou,
2000). Solarization reduced black dot of potato caused by Colletotrichum
coccodes by 45 percent when tarping was done for eight weeks and temperatures reached 56C in the top 5 cm of soil. With a six-week tarping period
and lower maximum temperature (50C), there was no significant reduction
in disease incidence (Denner et al., 2000). Soil solarization reduced severity
of diseases caused by Verticillium spp. on eggplant and Phytophthora spp.
on snapdragons (Pinkerton et al., 2000). Agrobacterium spp. population
densities declined within solarized plots, and incidence of crown gall on
cherry rootstock planted in solarized plots was reduced significantly (Pinkerton et al., 2000).
ORGANIC AMENDMENTS
Root rots are known to be greater in number and more severe in soil with
low organic matter. Incorporation of organic matter reduced root rot in
many crops (Tu, 1987). Addition of liquid swine manure to field soils killed
Verticillium dahliae microsclerotia and reduced Verticillium wilt of potato

(Conn and Lazarovits, 2000). The efficacy of the swine manure was dependent on soil pH. Adjusting the soil pH from 5.0 to 6.5 eliminated the toxicity of the swine manure. Conversely, when soil from a location in which
swine manure had no effect was reduced from its initial pH value of 7.5 to
below 6, swine manure became effective in that soil (Conn and Lazarovits,
2000). Fresh chicken manure, or chicken manure composted for five weeks
before incorporation into the potting mix (25 percent, vol./vol.), significantly reduced Phytophthora cinnamomi survival and the development of
symptoms on Lupinus albus seedlings (Aryantha et al., 2000).
Addition of composts to the field reduces the disease incidence. However, the composting method may determine the efficacy of the composts.
Composts were prepared by three different methods (Kannangara et al.,
2000). Two of the composts were prepared from separated dairy solids either by windrow (WDS) or vermicomposting (VMC) while the third, obtained from the International Bio Recovery (IBR), was prepared from vegetable refuse using aerobic digestion. Amendment of WDS in the potting mix
suppressed Fusarium oxysporum f. sp. radicis-cucumerinum infection on
cucumber, while VMC and IBR had no effect. There was a large increase of
fluorescent bacteria near the vicinity of roots particularly in WDS amended
potting mixes. Contrasting effectiveness of the WDS and VMC made from
the same waste suggests that composting method can influence the disease
suppression properties of the finished compost (Kannangara et al., 2000).
Compost-amended potting mixes are used to manage soilborne diseases
of floricultural crops caused by Pythium, Phytophthora, Fusarium, and
Rhizoctonia. Several biocontrol agents such as Trichoderma spp., Gliocladium sp., fluorescent pseudomonads, and Bacillus spp. colonize the
compost (Hoitink et al., 1991). Pine bark is used for preparing compost on a
commercial scale. The raw pine bark is first hammer milled and screened so
that all particles are 1.25 cm in diameter. A small amount of nitrogen (0.6
kg/m3 of bark), mostly as ammonia or urea, is added with water as the first
step in the composting process. The wet and nitrified bark is stored in windrows on a concrete pad and turned several times during an eight- to elevenweek period. Temperature, electrical conductivity, pH, and moisture levels
are monitored regularly to follow the type of composting. Adjustments are
made to maintain optimum conditions for the process (Hoitink et al., 1991).
A computer-controlled composting system has also been developed. This
type of compost is used at incorporation rates of up to 25 percent. These
mixes should be stored in 100 L polyethylene bags or in 2 m3 nylon bags for
days to months before their use. At least four days must be allowed for
recolonization of high-temperature (40-50C) compost with a mesophilic
microflora to induce the natural suppression of Pythium damping-off (Hoit-
ink et al., 1991). Growers in the eastern United States have consistently suppressed Fusarium wilt of cyclamen for over a decade by using composted
pine bark mixes (Hoitink et al., 1991).
Incorporation of plant residues into soil suppresses certain soilborne diseases. The efficacy of various organic amendments for controlling soilborne
pathogens has been attributed to the formation of toxic volatile compounds
or to an increase in antagonistic soil microflora (Pinkerton et al., 2000).
Brassicas, which contain glucosinolates that break down to toxic isothiocyanates, and Sudan grasses, which contain dhurrin that breaks down to
hydrogen cyanide, have been reported to control fungal pathogens. Soil
amendment with brassicas has a beneficial effect on the control of some
soilborne pathogens. Residues from brassica crops could be incorporated
directly into the soil or brassicas could be used as intercrops (Rosa and
Rodrigues, 1999). Cauliflower wilt caused by Verticillium dahliae was effectively controlled by incorporating broccoli (B. oleracea L. var. italica)
residues in soil in Salina Valley, California (Koike and Subbarao, 2000).
Broccoli residue showed a detrimental effect on the viability of microsclerotia in soil. It also had an inhibitory effect on the root-colonizing potential of surviving microsclerotia (Shetty et al., 2000). Air-dried and crushed
mustard (Brassica juncea) added to the soil effectively reduced the viability
of sclerotia of Sclerotium cepivorum, the onion white rot pathogen, and
chlamydospores of Fusarium oxysporum f. sp. lycopersici, the tomato wilt
pathogen (Smolinska, 2000). Consequently, the reduction of white rot of
onion and tomato wilt was observed. The addition of rapeseed (Brassica
napus) residues to soil also resulted in a decrease of number of sclerotia of
S. cepivorum (Smolinska, 2000). Amendment of soil with dried leaves of
savoy cabbage (Brassica oleracea var. sabauda), red cabbage (B. oleracea
var. capitata f. rubra), and fringed cabbage (B. oleracea var. acephala) significantly reduced the appearance of cucumber damping-off caused by
Pythium ultimum (Burgiel and Schwartz, 2000). The dried leaves possessed
fungistatic activity against P. ultimum (Burgiel and Schwartz, 2000).
The effect of cruciferous plant residues in reducing the disease incidence
may be due to the glucosinolate content of these plants. Aphanomyces
euteiches root rot of pea was significantly reduced (77 percent) in soil
amended with rapeseed meal from Brassica napus cv. Dwarf Essex (high
glucosinolate concentrations). Amendment with cv. Stonewall (low glucosinolate concentrations) did not control pea root rot (Dandurand et al.,
2000), suggesting that a low concentration of glucosinolate may not be sufficient to control the pathogen in soil. The toxicity of glucosinolates may
probably be due to isothiocyanates derived from the breakdown of glucosinolates (Rosa and Rodrigues, 1999).
Fresh broccoli or grass was incorporated into soil and covered with plastic sheeting. In this amended soil, anaerobic and strongly reducing soil conditions developed quickly, as indicated by rapid depletion of oxygen and a
decrease in redox potential values to as low as 200mV. After 15 weeks, survival of Fusarium oxysporum f. sp. asparagi, Rhizoctonia solani, and Verticillium dahliae in inoculum samples buried 15 cm deep was strongly reduced in amended, covered fields. The pathogens were not or hardly
inactivated in amended, noncovered soil or nonamended, covered soil,
indicating that inhibitory action of fresh leaves or thermal inactivation due
to increased soil temperatures under the plastic cover was not involved in
pathogen inactivation (Blok et al., 2000).
MULCHING
Mulches are different from soil amendments in that amendments are incorporated into the soil, while mulches are spread on the soil surface.
Mulches may consist of straw or stubble applied to the soil surface, or of
plastic foil spread over the soil. Mulches are known to reduce various diseases. Silver-painted mulch suppressed yellow mosaic virus symptoms in
squash, while nonreflective (black) mulch did not have any effect on the disease incidence. The reflective mulch was effective only under slight virus
pressure (Boyhan et al., 2000). Reflective mulch significantly reduced the
vector thrips and Tomato spotted wilt virus in tomato (Riley and Pappu,
2000). Black plastic mulch reduced the Verticillium wilt symptoms in eggplant (Elmer, 2000).
LIVING GROUND COVERS
Living covers, such as perennial peanuts (Arachis pintoi), cinquillo
(Drymaria cordata), and coriander (Coriandrum sativum), grown along
with tomato mask the tomato crop from immigrating viruliferous whiteflies
(Bemisia tabaci). These covers minimize contact between the vector and the
tomato plant. The living covers reduce whitefly adult numbers, delay Tomato yellow mottle virus dissemination, reduce disease severity, and provide higher yields in tomatoes (Hilje, 2000). The forage groundnut Arachis
pintoi was grown as a ground cover with bell pepper (Capsicum annum).
Approximately four times as many whiteflies were observed on bell pepper
plants grown without ground cover than on plants with cover. Onset of the
gemini virus disease in bell pepper was earlier and frequency was greater in
plants without cover than in plants with cover (Rafie et al., 1999).
CATCH CROPS
Control of some soilborne pathogens may be achieved by use of decoy or
catch crops. These crops stimulate the germination of resting spores, resulting in limited expression of disease symptoms. Chinese cabbage plants
grown in pots inoculated with Plasmodiophora brassicae (the clubroot
pathogen) that had previously contained leafy daikon (radish, Raphanus
sativus var. longipinnatus) showed less disease incidence compared to control pots in which no plants had been grown before. Numbers of resting
spores of P. brassicae in soil in pots after cultivation with leafy daikon were
reduced by 71 percent compared to control pots (Murakami et al., 2000).
TILLAGE
Some tillage operations may help to reduce disease incidence. Moldboard plowing to a depth of 30 cm reduced black dot of potato caused by
Colletotrichum coccodes by 34 percent and was twice as effective as plowing to a depth of 60 cm (Denner et al., 2000). Fall chisel plowing plus spring
raised seedbed preparation significantly reduced pea root rot (Fusarium
solani f. sp. pisi and F. oxysporum f. sp. pisi) severity, compared to conventional fall plowing plus spring flat seedbed preparation. Root rot was most
severe with fall plowing plus compaction, followed by fall plowing plus fall
seedbed preparation (Tu, 1987). Reduced tillage that leaves 30 percent or
more of the soil surface covered by crop residue after planting completely or
partially controls several wheat root rot and foot rot pathogens, such as
Bipolaris sorokiniana, Fusarium graminearum, F. culmorum, F. avenaceum, and Pseudocercosporella herpotrichoides. Root rot is favored by
drought stress. The increased moisture available to the crop under reduced
tillage might have led to reduction in disease incidence (Bockus and Shroyer,
1998). The prevalence of Sclerotinia stem rot (Sclerotinia sclerotiorum) of
soybeans was less in no-till than in minimal-till fields in the north central
United States (Workneh and Yang, 2000).
SPACING
Spacing between plants plays an important role in disease incidence.
Wider plant spacing reduces Botrytis fruit rot incidence in strawberry
(Legard et al., 2000). Good aeration around host tissues often minimizes the
incidence and severity of diseases caused by B. cinerea (Cooley et al.,
1996). Paired rows, as opposed to evenly spaced rows, reduce wheat root
rots by allowing the top layer to dry more rapidly because of openness between every other row of plants (Cook and Veseth, 1991). It has been suggested that opening the trees to sunshine and air should be the first measure
taken to control sooty blotch and flyspeck of apple caused by four different
fungi (Williamson and Sutton, 2000). Pruning creates an environment less
favorable to the diseases. The sooty blotch and flyspeck could be reduced by
an average of almost 30 percent by severe pruning (Williamson and Sutton,
2000).
MANIPULATION OF SOIL pH
Potato common scab (Streptomyces scabies) can be suppressed by lowering the soil pH. When ammonium sulfate was applied into the rows where
potato plants were to be planted, the soil pH was lowered and the concentration of water-soluble aluminium was increased. Potato common scab was
suppressed in the soil containing water-soluble aluminium in concentrations of 0.2 to 0.3 mg/liter or higher (Mizuno et al., 2000).
INORGANIC AMENDMENTS
Several inorganic compounds alter the severity of disease incidence in
the field. Infection of avocado seedlings by Phytophthora cinnamomi in
infested soil was decreased by 71 percent by the addition of gypsum soil
amendments (Messenger et al., 2000b). Sporangial production of P. cinnamomi buried in gypsum-amended avocado soil for two days was reduced
by as much as 74 percent (Messenger et al., 2000a). Soil extracts from gypsum-amended soil (1, 5, or 10 percent gypsum) reduced in vitro sporangial
production. Zoospore production and colony-forming units of P. cinnamomi
were reduced in soil amended with calcium sulfate, calcium nitrate, or calcium carbonate. It suggests that calcium may be playing a key role in suppressing sporulation and multiplication of the pathogen in soil (Messenger
et al., 2000a).
Calcium application effectively controlled bacterial wilt of tomato caused
by Ralstonia solanacearum (Yamazaki et al., 2000). Adding CaCO3, Ca(OH)2,
and CaSO4 to the soil reduced wilt of banana caused by Fusarium oxysporum f. sp. cubense. Smaller amounts had the greatest effect, and the
amounts of calcium compounds used were insufficient to change the pH.
Calcium treatment has been shown to inhibit germination of chlamydospores of the pathogen and suggests that calcium may act directly on the
pathogen (Peng et al., 1999).
High nitrogen application increases Rhizoctonia stem canker in potato;

less nitrogen and high potassium and phosphorous application results in decrease in the disease incidence (Crozier et al., 2000). Nitrogen application
may have different actions on different pathogens in a host (Hoffland et al.,
2000). N application increased susceptibility to the bacterial speck pathogen Pseudomonas syringae pv. tomato and the powdery mildew pathogen
Oidium lycopersicum in tomato. It did not have any effect on the wilt pathogen Fusarium oxysporum f. sp. lycopersici. However, increased application
of nitrogen increased resistance to the gray mold pathogen Botrytis cinerea
(Hoffland et al., 2000).
Different forms of nitrogen may affect the disease incidence differently.
The ammoniacal form of nitrogen, as opposed to the nitrate form, reduces
wheat take-all disease by producing a more acidic root surface, inhibiting
the alkaline-loving pathogen Gaeumannomyces graminis (Huber and McCayBuis, 1993). Fertilization with (NH4)2SO4 showed less Verticillium symptoms in eggplant than with Ca(NO3)2 fertilization (Elmer, 2000).
CROP ROTATION AND INTERCROPPING
Diseases can be managed by proper planning of the crop sequence. A rotation including barley, field peas, and wheat for three years following canola (Brassica napa) helped to eliminate potential disease sources in canola
(Leptosphaeria maculans) in Canada (Turkington et al., 2000). Rotation to
grain sorghum to produce a one-year break from wheat resulted in effective
control of tan spot of wheat. This was true even when the maximum amount
of wheat residue was retained on the soil surface (without tillage). The time
during the rotation in which there is no susceptible host present may serve to
sensitize the soil by utilizing the native microorganisms to weaken and kill
residue-borne pathogens (Bockus and Shroyer, 1998). Soils from two orchards in the United States were cultivated with three successive 28-day
growth cycles of wheat in the greenhouse and subsequently planted to apple
seedlings. Cultivation of wheat in orchard replant soils prior to planting of
apple seedlings resulted in reduction in apple root infection by Rhizoctonia
and Pythium spp. In replant soils cultivated with wheat, the antagonistic
bacteria Pseudomonas putida population dominated (Mazzola and Gu,
2000).
Strawberries are grown as green manure in Italy. Sown in autumn, the
strawberry crop is cut and buried when in full flower. It is useful for management of diseases of Brassica juncea grown after strawberry. The hydrolyzed glucosides from strawberry are toxic to Phytophthora, Pythium,
Rhizoctonia, and Sclerotium species (Frabboni, 2001). Fusarium oxysporum
f. sp. apii infection in celery is suppressed with amendments of onion or

peppermint crop residues. Celery grown after an onion crop is less affected
by the Fusarium yellows fungus (Lacy et al., 1996).
During four consecutive years of experiments in the Netherlands with
carrots grown as sole crops or undersown with subterranean clover (Trifolium subterraneum), it was shown that intercropping significantly reduced
the cavity spot caused by Pythium spp. (Theunissen and Schelling, 2000).
IRRIGATION
Irrigation practices determine the severity of disease incidence in many
crops. Overhead sprinkling irrigation enhanced production of oospores of
Phytophthora infestans in field-grown crops of potato (Cohen, Farkash,
et al., 2000). Frequent irrigation increased the Verticillium wilt incidence in
cauliflower. Deficit irrigation suppressed the wilt incidence; however, it also
reduced the yield (Xiao and Subbarao, 2000). Hence, irrigation methods
should be properly planned to control diseases without any reduction in
crop yield. When compared to pipe irrigation, trickle irrigation (at a depth of
30 cm) reduced damage by plant diseases and increased crop yields of both
fennel and cauliflowers in Germany (Eberhard, 2000). Daily irrigation increased Monosporascus wilt of melons, while in a less frequently irrigated
field, symptom development was delayed and disease incidence was decreased (Cohen, Pivonia, et al., 2000).
HYDROPHOBIC PARTICLE FILMS
Hydrophobic particle film technology has been exploited to manage diseases. Hydrophobic particle film is based on the inert mineral kaolin, which
is surface treated with a water-repelling agent. Dust application of hydrophobic particles on plant surfaces results in envelopment of the plant in a hydrophobic particle film barrier that prevents disease inoculum or water from
directly contacting the leaf surface. By this method, disease development is
suppressed. This technology could offer broad-spectrum protection against
several diseases in agricultural crops (Glenn et al., 1999).
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27
Exclusion
Exclusionand
and Eradication
Eradication
The most effective method to manage diseases is to exclude them from
the field. Quarantine is important in preventing the spread of pathogens
across countries, and legislative control is useful to prevent the entry of a
pathogen into a new region. If a pathogen has entered into a region, eradication of the established infection is practiced. Because most diseases are
spread internationally through seed, seed health testing and seed certification help to prevent the spread of diseases. Various seed health testing methods are described in this chapter.
EXCLUSION
Quarantine services exist in most countries. Plant quarantine is legislative or regulatory control that aims to exclude pathogens from areas where
they do not already exist. Legislative control may operate on a national or
international level. The legislation prohibits or restricts the introduction of
seeds, vegetative propagating materials, plants, or plant parts into a country
or a region in a country to exclude pathogens, which may be inadvertently
introduced along with those materials. Generally, scientists, the traveling
public, and some importers of agricultural products are responsible for the
introduction of new pathogens into a region.
Not all pathogens are of quarantine significance. A pathogen species that
does not occur in a given country or an exotic strain of a domestic species is
of quarantine significance to that country if the pathogen is known to cause
economic damage elsewhere or has a life cycle or host/pathogen interaction
that shows a potential to cause economic damage under favorable host,
inoculum, and environmental conditions (Kahn, 1991). Importation of a
pathogen that already occurs in a given country is also of quarantine significance if an ongoing regional or national containment, suppression, or eradication program is directed against that pathogen species.
Actions to be taken to exclude pathogens are authorized by government
regulations. In the United States, the Plant Quarantine Act was enacted in
1912. The act provides authority for domestic and foreign quarantines. The
Organic Act of 1944 authorizes the secretary of agriculture to cooperate

with states, organizations, and individuals to detect, eradicate, suppress,
control, and prevent or retard the spread of plant pests (including pathogens). The Federal Plant Pest Act was enacted in 1957 and regulates the
movement (by persons) of plant pests into United States or between states
and authorizes emergency actions to prevent the introduction and domestic
movement of plant pests not covered by the Plant Quarantine Act of 1912
(Kahn, 1991).
Transport of plant material across international boundaries is regularized
by the International Plant Protection Convention of 1951. The convention
was organized by the United Nations Food and Agriculture Organization
(FAO) with the aim of securing common and effective action to prevent the
introduction and spread of pests and diseases of plants and plant products.
The convention was signed by 94 nations and conformed to by most other
countries. The phytosanitary certificate is an instrument of that treaty. The
convention is now regionally organized. In Europe and the Mediterranean,
the organizational body is the European and Mediterranean Plant Protection
Organization (EPPO), and in North America, it is the North American Plant
Protection Organization (NAPPO). These organizations regularly issue bulletins including information on newly identified pests and pathogens and
phytosanitary regulations (Parry, 1990).
Most member countries have their own disease legislation with regard to
imported plant material. Some countries have formed unions, such as the
European Economic Community, and promulgate binding regulations on
member countries. The legislation prescribes the form of health certificate
to accompany any imported material. It lays down rules for inspection and
disposal of material if it contains pathogens. It also provides a list of prohibited imports and a list of restrictions of imports of material from specified
areas. In the United Kingdom, Plant Health Order 1987 lists import restrictions into the United Kingdom.
The legislation is normally implemented by customs and excise officers.
They check the documentation, and specifically phytosanitary certificates,
at ports. Spot checks are carried out by officers of the Plant Health and
Seeds Inspectorate on material both entering and leaving the United Kingdom. Suspect materials are put into quarantine for a period of time to detect
pathogens, which are present in seed and planting materials.
In spite of quarantine methods, a few pathogens have entered into countries that had not reported the occurrence of such pathogens earlier, shattering the economy of those countries. Rust disease of coffee (Coffea arabica
and C. canephora) wiped out coffee plantations in Sri Lanka in 1880. The
disease spread to Central and East Africa by the 1920s and to West Africa in
the 1950s. It became severe in Brazil in 1970, and the disease is now preva-
lent in Mexico, Honduras, Paraguay, Argentina, Peru, Bolivia, Guatemala,

Colombia, Costa Rica, India, and almost all countries where coffee is
grown. Fire blight of apple is widespread in North America and it is not a
quarantine object in the United States. However, in Europe, fire blight of apple is not prevalent in many countries. The disease was first detected in
Spain in 1995 and in Hungary in 1996.
Karnal bunt of wheat was first discovered in 1930 at Karnal, a small town
in the Haryana state in India. It was subsequently reported in countries
around India, Pakistan, Nepal, Afghanistan, and Iraq. It was first reported in
Mexico in 1972. In the United States, the disease was first discovered in Arizona in 1996. Subsequently, the disease has been reported in Texas, New
Mexico, and California. The Mexican government placed an internal quarantine on Karnal bunt to prevent disease spread within the country in 1984.
In 1996, a federal quarantine for Karnal bunt was placed on the states of Arizona, Texas, New Mexico, and California. The U.S. Department of Agriculture (USDA) Animal and Plant Health Inspection Service (APHIS) prohibited importation of seed, grain, straw, and dried plants of wheat, durum, and
triticale from Mexico to prevent the entry of Karnal bunt into the United
States. Mexico was permanently added to the list of the wheat disease
subpart of Quarantine 319 (Babadoost, 2000). A zero-tolerance level of
Karnal bunt has been enforced in the United States, Canada, and many other
countries. The certification standard in India is zero incidence. APHIS tests
the presence of teliospores in wheat seed samples by the centrifuge wash
test. Test tubes containing seeds submerged in water are shaken for ten minutes to obtain teliospore suspension, then centrifuged for 20 minutes at
3,000 rpm and the sediment examined under a microscope for the presence
of teliospores (Warham, 1992). The size-selective sieving test is also recommended (Peterson et al., 2000). A seed wash of a 50 g seed sample is washed
through 50 M and 20 M pore size nylon screens to remove unwanted debris and to concentrate and isolate teliospores. The material remaining in
the 20 M pore size is suspended for direct microscopic examination and
identified by polymerase chain reaction (PCR) utilizing two pairs of Tilletia
indica-specific primers.
ERADICATION
National legislation also enforces eradication of exotic pathogens recently introduced along natural or man-made pathways. Canker is the most
serious disease in Citrus spp. It was first reported in 1913 in Florida, and an
extensive citrus canker eradication program was implemented there in
1915. After $6 million had been spent for eradication, Florida was declared
free from the disease in 1933. The eradication program was also taken up in
Georgia, Alabama, Louisiana, South Carolina, Texas, and Mississippi. In
1947, citrus canker was declared to be eradicated from these states. However, a new form of the disease appeared in 1984 in Florida. Another eradication program was implemented, and by 1991, over 20 million trees had
been destroyed at a cost of about $94 million dollars. The Asiatic citrus canker is still prevalent in different parts of the United States (Gottwald et al.,
2001). The pathogen of fire blight of apple (Erwinia amylovora) entered
Hungary in 1996. In 1997 and 1998, further spread of the disease was registered and an eradication program was launched. More than 60,000 trees
were uprooted and destroyed across the country. Eradication was performed
partly by special brigades and partly with participation of growers. Erwinia
amylovora was first detected in 1995 in Spain, and several measures were
taken to eradicate the bacteria there as well.
In the United Kingdom, national plant disease legislation has been introduced to eradicate specific pathogens. It makes farmers responsible to inform officials about outbreaks of indigenous but geographically localized
diseases, known as notifiable diseases. Fire blight of apples and pears
(Erwinia amylovora), wart disease of potatoes (Synchytrium endobioticum), brown rot (Ralstonia solanacearum) and ring rot (Clavibacter michiganensis ssp. sepedonicus) of potato, plum pox disease of plums (Plum pox
virus), red stele disease of strawberries, rhizomania disease of beet (Beet necrotic yellow vein virus), and progressive wilt of hops (Verticillium alboatrum) are the important notifiable diseases in the United Kingdom. Occurrence of such diseases must be reported. The diseased material should not
be transported or sold and must be destroyed.
LIST OF IMPORTANT SEEDBORNE PATHOGENS
Several fungal, bacterial, viral, and phytoplasmal diseases are transmitted through seeds, including vegetative propagules (Mink, 1993; Johansen
et al., 1994; Langerak et al., 1996). The important seedborne pathogens are
listed in this section.
AlfalfaAlfalfa mosaic virus
BarleyBarley stripe mosaic virus, Xanthomonas campestris pv.
translucens, Xanthomonas campestris pv. undulosa, Rhynchosporium
secalis, Ustilago segetum var. nuda, Pyrenophora teres
BeanBean common mosaic virus, Bean pod mottle virus, Bean southern
mosaic virus, Bean yellow mosaic virus, Pseudomonas savastanoi pv.
phaseolicola, Curtobacterium flaccumfaciens pv. flaccumfaciens,
Xanthomonas axonopodis pv. phaseoli, Pseudomonas syringae pv.

syringae, Colletotrichum lindemuthianum
BeetPhoma betae
BlackgramUrd bean leaf crinkle virus, Blackgram mottle virus
Broad beanBroad bean mottle virus, Broad bean true mosaic virus,
Broad bean stain mosaic virus, Broad bean wilt virus
CarrotXanthomonas campestris pv. carotae, Alternaria dauci,
A. radicina
CelerySeptoria apiicola
CherryCherry leaf roll virus, Cherry rasp leaf virus, Cherry X-disease
CornMaize chlorotic dwarf virus, Maize mosaic virus, Erwinia
stewartii, Fusarium moniliforme, Peronosclerospora sacchari,
Peronosclerospora sorghi, Sclerospora graminicola
CottonXanthomonas axonopodis pv. malvacearum, Colletotrichum
gossypii
CowpeaBlackeye cowpea mosaic virus, Cowpea aphid borne mosaic virus, Cowpea banding mosaic virus, Cowpea mild mottle virus, Cowpea
mosaic virus, Cowpea ringspot virus, Cowpea severe mosaic virus
CrucifersXanthomonas campestris pv. campestris, Phoma lingam,
Alternaria brassicicola, Leptosphaeria maculans
CucumberCucumber mosaic virus, Cucumber green mottle mosaic
virus
EggplantEggplant mosaic virus
FlaxAlternaria linicola
GrapevineGrapevine fan leaf virus, Grapevine Bulgarian latent virus
LettuceLettuce mosaic virus
MelonMelon necrotic spot virus, Muskmelon necrotic ringspot virus
Mung beanMungbean mosaic virus
OatsOat mosaic virus, Pyrenophora avenae
OnionOnion yellow dwarf virus
PeaPea early-browning virus, Pea enation mosaic virus, Pea seedborne
mosaic virus, Pseudomonas syringae pv. pisi, Ascochyta pisi
PeachPeach rosette mosaic virus, Prunus necrotic ringspot virus, Prune
dwarf virus, Peach X-disease
PeanutPeanut clump virus, Peanut mottle virus, Peanut stripe virus,
Peanut stunt virus
Pearl milletSclerospora graminicola
PlumPlum pox virus
PotatoPotato virus X, Potato virus Y, Potato virus T, Potato spindle tuber viroid
RaspberryRaspberry ringspot virus, Raspberry bushy dwarf virus
Red cloverRed clover mottle virus, Red clover vein mosaic virus
RiceXanthomonas oryzae pv. oryzae, X. oryzae pv. oryzicola,

Burkholderia glumae, Pseudomonas fuscovaginae, Alternaria
padwickii, Cochliobolus miyabeanus, Pyricularia oryzae, Tilletia indica, Fusarium moniliforme
SorghumPeronosclerospora sorghi, Sclerospora sorghi, Sporisorium
sorghi, Sporisorium cruentum, Claviceps sorghi
SoybeanSoybean mosaic virus, Pseudomonas savastanoi pv. glycinea,
Cercospora kikuchii, Diaporthe phaseolorum
SpinachSpinach latent virus
SquashSquash mosaic virus
StrawberryStrawberry latent ringspot virus
Subterranean cloverSubterranean clover mottle virus
SunflowerSunflower mosaic virus
TobaccoTobacco etch virus, Tobacco mosaic virus, Tobacco rattle virus, Tobacco ringspot virus, Tobacco streak virus
TomatoTomato aspermy virus, Tomato black ring virus, Tomato bushy
stunt virus, Tomato ringspot virus, Tomato spotted wilt virus,
Clavibacter michiganensis ssp. michiganensis, Pseudomonas syringae
pv. tomato, Fusarium oxysporum f. sp. lycopersici
TurnipTurnip yellow mosaic virus
WatermelonWatermelon mosaic virus
WheatWheat streak mosaic virus, Wheat striate mosaic virus,
Xanthomonas campestris pv. translucens, Xanthomonas campestris pv.
undulosa, Ustilago nuda, Tilletia caries, Tilletia controversa
White cloverWhite clover mosaic virus
SEED HEALTH TESTING AND CERTIFICATION
Several countries have enacted laws for the certification of seeds (including propagating materials) free from pathogens. For example, in the United
Kingdom, it is illegal to sell the seed of major agricultural and horticultural
crops unless it has been certified as meeting specified minimum standards
of quality, including freedom from disease. Crops are inspected by trained
inspectors both in the field and after harvest. However, this is not sufficient
to identify the pathogen, which persists in symptomless crops and seeds.
Seed health testing has become important in many countries in recent
years. For example, in Canada, until the early 1970s, only visual field inspections of growing potato crops and harvested tubers served to identify
Clavibacter michiganensis ssp. sepedonicus-infected lots that needed to be
removed from the seed certification program. In 1979, laboratory testing to
detect the possible presence of C. michiganensis ssp. sepedonicus in seed
lots that had passed field inspection was initiated in Canada to facilitate international trade, because it was a pathogen of quarantine significance (De
Boer and Hall, 2000). By 1985, the advantage of laboratory testing for detecting incipient ring rot infections had become clear, and testing of domestic seed lots was introduced on a voluntary basis in some provinces. By
1992, laboratory indexing of all seed lots for C. michiganensis ssp. sepedonicus in Canada became mandatory. With privatization of potato testing
in Canada, an accreditation program was implemented to ensure that reliable and uniform results were obtained from multiple laboratories. The
quality-assurance program of each private laboratory must follow the criteria set by the International Standards Organization (ISO) in their guide. Analysts in private laboratories are required to complete correctly blind proficiency panel samples on a semiannual basis to maintain their certified
status, which allows them to conduct the tests in an accredited laboratory.
These proficiency tests are administered by the Centre of Expertise for Potato Diseases of the Canadian Food Inspection Agency (De Boer and Hall,
2000). Similar seed health testing laboratories are available in the United
States, Europe, and many Asian countries. The major purpose of these laboratories is to ensure the supply of seeds (including propagating materials)
free from pathogens to the growers in order to exclude pathogens from
fields, farms, regions, and countries.
SEED HEALTH TESTING METHODS
Guidelines for the standardization of seed health testing methods were
drafted at the first Workshop on Seed Health Testing of the technical Plant
Disease Committee (PDC) of the International Seed Testing Association
(ISTA), held in Cambridge in 1958 (Langerak et al., 1996). Since then, numerous plant pathologists have worked on the development and standardization of seed health testing methods. These methods were evaluated in
comparative testing programs of the PDC. The evaluated methods were
compiled and published by ISTA as working sheets. These working sheets
describe seed health testing methods for individual pathogens separately for
each host and are included in the ISTA Handbook on Seed Health Testing.
Standardization of seed health testing methods is important to provide
assurances to the seed user that adequate seed health testing was provided.
The International Seed Health Initiative (ISHI) was founded in 1993 to address the immediate need for an efficient standardization process to accommodate the international seed trade as well as the level of testing proficiency
required in the private sector for the international movement of seed. ISHI is
an international consortium of seed industry and seed health testing plant
pathologists from the United States, the Netherlands, France, Japan, and Israel (Maddox, 1998). ISHI supports the accreditation of private laboratories
to assure the quality assurance of the testing and provide a means for regulatory testing that is both efficient and acceptable for phytosanitary regulation. The members of ISHI are working for worldwide standards in seed
health in conjunction with ISTA and other regulatory agencies to provide a
database of acceptable testing methods for world phytosanitary goals (Maddox, 1998).
Common Seed Health Testing Methods
Blotter Tests
Seeds are placed on two to three layers of water-soaked blotter papers in
petri dishes and incubated under alternating light, provided by fluorescent
white or near ultraviolet (NUV) tube lights, and dark periods. Fructifications
of fungal pathogens developing on seeds are identified using stereoscopic
and/or light microscopes. In a modified blotter test, seeds are placed between several layers of moist paper and incubated either in darkness or exposed to a 12-hour photoperiod. Specific symptoms may develop on germinating seedlings or characteristic fructifications may develop on the seed
coat, which can be identified by microscopic inspection. In the 2,4-D blotter
test, seed germination is prevented to create conditions for development of
mycelium or spores of the pathogen on the seed itself. The herbicide 2,4-D
solution is added to the blotter, and seeds are incubated under alternating
fluorescent white or near ultra violet light and dark periods. The developing
fructifications on the seed coat can be identified by microscopic inspection.
In a modified 2,4-D blotter method, instead of adding 2,4-D, the moist blotter with seeds is frozen at 20C after pre-imbibition at 20C to prevent seed
germination, allowing development of fungal fructifications on the seed
coat. The incubation conditions of various blotter tests can be modified depending on the requirements for development of fructifications of individual pathogens (Langerak et al., 1996).
Seed Washing Method
The seed washing method involves placing individual seeds or portions
of seeds in water or water plus detergent to promote release of spores or
conidia. Staining techniques are employed to distinguish between closely
related species of Tilletia in wheat. The repetitive-sequence-based polymerase chain reaction (rep-PCR) method is also useful (McDonald et al., 2000).
Embryo Staining Test

This test is used to detect Ustilago tritici in wheat and involves visual inspection of internal parts of the seed after separation, clarification, and
staining of mycelium fragments in the seed tissue.
Agar Tests
In this method, seeds are plated on agar media containing nutrients. Selective media are also used to identify some specific pathogens. Surface
sterilization of seeds with sodium hypochlorite is needed to avoid development of surface contaminants in the agar medium, but it may also inhibit development of pathogens present on the seed coat. Incubation conditions,
such as temperature and exposure to light, also determine the development
of pathogens on seeds plated on agar media.
Grow-Out (or Growing-On, Seedling Symptom) Tests
Seeds are grown in agar media in test tubes or in sand/soil in pots and incubated under different light and temperature conditions. Development of
disease symptoms on seedlings is assessed.
Seed Extract and Dilution Plating
Seedborne bacteria are separated from seeds by soaking, washing, or extraction after crushing or maceration of the seed. The seed extract is then analyzed for the presence of pathogenic bacteria by dilution plating on selective media.
Serological and Nucleic Acid Probe-Based Methods
Recently, serological techniques and DNA-based methods have been developed. These techniques are mostly used to detect viral and bacterial
pathogens. The important tests used are the latex agglutination test, the
immuno-diffusion test, the microprecipitin test, enzyme-linked immunosorbent assay (ELISA), the immunoblot test, immunofluorescence, dotimmunobinding assay, enzyme-linked fluorescent assay, immunosorbent
electron microscopy, radio immunosorbent assay, polymerase chain reaction, and DNA hybridization on DNA extracted from seeds. All these methods have been described in detail in Chapter 13, Crop Disease Diagnosis.
The commonly used seed health testing methods to detect various pathogens (Langerak et al., 1996; Maddox, 1998) follow:
Wheat
Tilletia caries and T. controversawashing test, repetitivesequence-based polymerase chain reaction
Ustilago triticiembryo staining test
Tilletia indicaNaOH soak test, washing test
Stagonospora nodorumagar test, blotter test, growing-on test, fluorescence test, agar-fluorescence test
Xanthomonas translucens pv. translucensdilution plating, dot
immunobinding assay
Barley
Barley stripe mosaic viruslatex agglutination test,
immunodiffusion test, immunosorbent electron microscopy,
ELISA
Ustilago segetum var. nudaembryo staining test
Xanthomonas translucens pv. translucensdilution plating, dotimmunobinding assay
Pyrenophora teresblotter test, agar test, growing-on test, deepfreezing test
Rhynchosporium secalisPCR (Lee et al., 2001)
Rice
Alernaria padwickiiagar test, blotter test
Cochliobolus miyabeanusblotter test
Pyricularia oryzaeblotter test
Fusarium moniliformeagar test and blotter test
Tilletia indicasodium hydroxide soak test
Xanthomonas oryzae pv. oryzaegrowing-on test, direct immunofluorescence, dilution plating
Xanthomonas oryzae pv. oryzicolagrowing-on test, direct immunofluorescence, dilution plating
Tomato
Clavibacter michiganensis ssp. michiganensis
immunofluorescence with seedling inoculation test, dilution plating, indicator host inoculation, seed wash/liquid plating, PCR
Pseudomonas syringae pv. tomatogrowing-on test, plating enriched seed extract
Xanthomonas vesicatoriadilution plating, immunofluorescence
combined with dilution plating, plating enriched seed extract
Fusarium oxysporumagar test
Tobacco mosaic virusindicator plants
Soybean
Cercospora kikuchiiagar test, blotter test
Diaporthe phaseolorumagar test
Peronospora manshuricawashing test
Phomopsis spp.blotter test, ELISA, immunoblot test
Pseudomonas savastanoi pv. glycineagrowing-on test, direct plating, host inoculation, seed wash/liquid plating, immunoassays
Tobacco ringspot virusELISA, immunosorbent electron microscopy
Bean
Colletotrichum lindemuthianumblotter test
Curtobacterium flaccumfaciensimmunofluorescence, seedling
inoculation test, growing-on test
Pseudomonas savastanoi pv. phaseolicoladilution plating,
immunofluorescence test, immunofluorescence colony staining
Xanthomonas axonopodis pv. phaseoliseed wash and host inoculation, seed wash and dilution plating, immunofluorescence test,
immunofluorescence colony staining, DNA hybridization, PCR
with seed extract
Bean common mosaic virusELISA, dot-immunobinding assay,
immunosorbent electron microscopy, microprecipitin test
Crucifers
Xanthomonas campestris pv. campestrisdirect plating,
immunofluorescence test, seed wash/liquid plating plus pathogenicity test
Phoma lingamdeep freezing blotter test

Alternaria brassicicolaseedling symptom test
Leptosphaeria maculans2,4-D blotter, freezing blotter, PCR with
DNA extract from seeds
Cucurbits
Squash mosaic virusELISA, grow-out test
Melon necrotic spot virusELISA
Cucumber green mottle virusELISA
Acidovorax avenae ssp. citrulligrow-out test, PCR, immunomagnetic separation and PCR (Walcott and Gitaitis, 2000)
Lettuce
Lettuce mosaic virusELISA, growing-on test, indicator plant test
Sugar beet
Pleospora betaeagar test, blotter test
Peach
Prune dwarf virusELISA
Prune necrotic ringspot virusELISA
INDEXING PLANT PROPAGATION MATERIALS
Most fruit trees (woody crops) are vegetatively propagated. Use of
healthy planting materials will exclude pathogens from the orchard. In recent years, several molecular techniques have been developed to index the
plant propagation materials and bud wood materials. Saade and colleagues
(2000) have developed techniques for detection of Prunus necrotic ringspot
virus, Prune dwarf virus, and Apple mosaic virus in almond, apricot, cherry,
peach, and plum by multiplex reverse-transcription polymerase chain reaction (RT-PCR). This technique will be useful for the analysis of mother
plants in certification programs. Merighi and colleagues (2000) developed
polymerase chain reaction enzyme-linked immunosorbent assay (PCRELISA) for detection of Erwinia amylovora in pear. This test will be useful
to select nursery stocks and plant propagation materials free from the pathogen. Use of disease-free budwood helps to exclude Citrus tristeza virus
(CTV) in citrus cultivation. ELISA and double antibody sandwich-ELISA

are useful to detect CTV in propagation materials (Terrada et al., 2000). Lin
and colleagues (2000) have described an in situ immunoassay for detection
of CTV in citrus bud wood.
REFERENCES
Babadoost, M. (2000). Comments on zero-tolerance quarantine of karnal bunt of
wheat. Plant Dis, 84:711-712.
De Boer, S. H. and Hall, J. W. (2000). Proficiency testing in a laboratory accreditation program for the bacterial ring rot pathogen of potato. Plant Dis, 84:649-653.
Gottwald, T. R., Hughes, G., Graham, J. H., Sun, X., and Riley, T. (2001). The citrus canker epidemic in Florida: The scientific basis of regulatory eradication policy for an invasive species. Phytopathology, 91:30-34.
Johansen, E., Edwards, M. C., and Hampton, R. O. (1994). Seed transmission of viruses: Current perspectives. Annu Rev Phytopathol, 32:363-386.
Kahn, R. P. (1991). Exclusion as a plant disease control strategy. Annu Rev
Langerak, C. J., van den Bulk, R. W., and Franken, A. A. J. M. (1996). Indexing
seeds for pathogens. Adv Bot Res, 23:171-215.
Lee, H. K., Tewari, J. P., and Turkington, T. K. (2001). A PCR-based assay to detect
Rhynchosporium secalis in barley seed. Plant Dis, 85:220-225.
Lin, Y., Rundell, P. A., Xie, L., and Powell, C. A. (2000). In situ immunoassay for
detection of Citrus tristeza virus. Plant Dis, 84:937-940.
Maddox, D. A. (1998). Implications of new technologies for seed health testing and
the worldwide movement of seed. Seed Sci Res, 8:277-284.
McDonald, J. G., Wong, E., and White, G. P. (2000). Differentiation of Tilletia species by rep-PCR genomic fingerprinting. Plant Dis, 84:1121-1125.
Merighi, M., Sandrini, A., Landini, S., Ghini, S., Girotti, S., Malaguti, S., and Bazzi,
C. (2000). Chemiluminescent and colorimetric detection of Erwinia amylovora
by immunoenzymatic determination of PCR amplicons from plasmid pEA29.
Plant Dis, 84:49-54.
Mink, G. I. (1993). Pollen- and seed-transmitted viruses and viroids. Annu Rev
Parry, D. W. (1990). Plant Pathology in Agriculture. Cambridge University Press,
Cambridge, U.K.
Peterson, G. L., Bonde, M. R., and Phillips, J. G. (2000). Size-selective sieving for
detecting teliospores of Tilletia indica in wheat seed samples. Plant Dis, 84:9991007.
Saade, M., Aparicio, F., Sanchez-Navarro, J. A., Herranz, M. C., Myrta, A., Di
Terlizzi, B., and Pallas, V. (2000). Simultaneous detection of the three ilarviruses affecting stone fruits by nonisotopic molecular hybridization and multiplex reverse-transcription polymerase chain reaction. Phytopathology, 90:13301336.
Terrada, E., Kerschbaumer, R. J., Giunta, G., Galeffi, P., Himmler, G., and Cambra,
M. (2000). Fully recombinant enzyme-linked immunosorbent assays using
genetically engineered single-chain antibody fusion proteins for detection of
Citrus tristeza virus. Phytopathology, 90:1337-1344.
Walcott, R. R. and Gitaitis, R. D. (2000). Detection of Acidovorax avenae subsp.
citrulli in watermelon seed using immunomagnetic separation and polymerase
chain reaction. Plant Dis, 84:470-474.
Warham, E. J. (1992). Karnal bunt of wheat. In U.S. Singh, A. N. Mukhopadhyay,
J. Kumar, and H. S. Chaube (Eds.), Plant Diseases of International Importance,
Volume I, Prentice Hall, Englewood Cliffs, NJ, pp. 1-24.
28
Plant
PlantActivators
Activators
Several plant activators, which activate host defense mechanisms and
protect the plants against pathogens, have been reported. These activators
appear to activate defense genes in a manner different from the way host resistance genes activate defense genes, possibly using a different signal
transduction system. The practical utility of plant activators in crop disease
management is discussed in this chapter.
WHAT ARE PLANT ACTIVATORS?
Plants are endowed with several defense genes. However, most of these
genes are quiescent in healthy plants. Specific signals are required to activate them. The chemicals that activate the defense genes by providing signals are called plant defense activators or plant activators. Plant activators
induce systemic resistance against diseases. Induced systemic resistance
(ISR) has been defined as active resistance dependent on the host plants
physical or chemical barriers, activated by biotic or abiotic agents (Kloepper et al., 1992). Induction of ISR has been shown to be due to induction of
various defense genes (Vidhyasekaran, 1998, 2001; Vidhyasekaran et al.,
2000). The other form of induced resistance in plants, systemic acquired resistance (SAR), refers to a distinct signal transduction pathway that is mediated by salicylic acid and activates defense genes (Dong and Beer, 2000).
Salicylic acid and several synthesized analogs of salicylic acid, such as 2,6dichloroisonicotinic acid (INA), are known to activate SAR. In contrast to
SAR, which is mediated by salicylic acid, ISR is mediated by jasmonic acid
and ethylene (Dong and Beer, 2000). ISR appears to be induced by biocontrol bacteria, while SAR is induced by infection by pathogens and chemical inducers. There are many exceptions to these definitions. For example,
riboflavin, a chemical, activates defense mechanisms and induces resistance; however, the activation is not through the salicylic acid pathway
(Dong and Beer, 2000). Several authors prefer to call this type of resistance
induced by chemicals ISR (Kuc, 2001; Oostendorp et al., 2001). It has
been demonstrated that ISR may act through a signaling pathway different
from the ones operating in various host-pathogen interactions. Salicylic

acid and its synthetic mimics 2,6-dichloroisonicotinic acid, and benzo
(1,2,3) thiadiazole-7-carbothioic acid S-methyl ester (BTH) and jasmonic
acid are known to induce resistance in barley against the powdery mildew
pathogen Blumeria graminis f. sp. hordei. All the chemically induced genes
were not expressed during incompatible barleyB. graminis f. sp. hordei interactions with gene-for-gene relationships (Besser et al., 2000).
Pretreatment of plants with an inducing compound appears to incite the
plant to mount an effective defense response upon subsequent encounters
with pathogens, converting what would have been a compatible interaction
to an incompatible one (Tuzun, 2001). ISR induces constitutive accumulation of defense gene products prior to pathogen invasion. ISR is known to
function against multiple organisms, and there is no specificity observed in
the accumulation patterns of defense-related gene products when ISR is induced. Plants in which ISR has been activated appear to move from a latent
resistance state to one in which a multigenic, nonspecific form of resistance
is active (Tuzun, 2001). ISR is a plant defense state associated with an enhanced ability, or the so-called priming, to resist pathogen attack by stronger activation of cellular responses (Conrath et al., 2001). Plant activators
may have a dual role in plant defense-gene activation. First, they may activate some defense genes, which are quiescent. Second, they may not directly activate defense genes, but they may prime them so when the pathogens invade, the defense genes are activated (Conrath et al., 2001). For
example, -aminobutyric acid (BABA) induces systemic resistance, but it
did not induce the accumulation of mRNA of the SAR-associated PR-1
gene. In contrast, BABA potentiated the accumulation of PR-1 mRNA after
attack by virulent pathogenic bacteria (Zimmerli et al., 2000). Carpropamid
enhanced the accumulation of the phytoalexins momilactone A and sakuranetin in rice leaves subsequently inoculated with the blast pathogen Magnaporthe grisea (Araki and Kurahashi, 1999).
TYPES OF PLANT ACTIVATORS
INA
The plant activator 2,6-dichloroisonicotinic acid has been reported to induce systemic resistance against many diseases. INA was found to protect
rice (Metraux et al., 1991), bean (Dann and Deverall, 1995), barley (Kogel
et al., 1994; Besser et al., 2000), cucumber (Metraux et al., 1991), sugar beet
(Nielsen et al., 1994), and rose (Rosa sp.) (Hijwegen et al., 1996) against
several pathogens, and was also effective in inducing resistance against
P. syringae pv. tabaci in tobacco (Press et al., 1997) and systemic resistance
against Alternaria macrospora leaf spot in cotton (Colson-Hanks and Deverall, 2000). Two synthetic compounds structurally related to INA, Nphenylsulfony-l2-chlorosonicotinamide and N-cyanomethyl-2-chlorosoisonicotinamide were also reported to induce resistance in rice (Yoshida et al.,
1990; Seguchi et al., 1992).
INA has been shown to act via activation of plant defense mechanisms
and has a dual mode of action: induction of defense responses prior to infection and potentiation of defense responses postinoculation. In sugar beet,
INA does not induce -1,3-glucanase or chitinase but conditions the plant to
induce these pathogenesis-related (PR) proteins faster upon attack by Cercospora beticola (Nielsen et al., 1994). Similar results were found in INAtreated cucumber hypocotyls upon challenge by Colletotrichum lagenarium
(Siegrist et al., 1994). INA may induce salicylic acid, which may signal the
induction of defense genes. SAR was characterized by activation of socalled SAR genes (Ward et al., 1991). Application of salicylic acid induces
SAR and the accumulation of defense genes (Uknes et al., 1992, 1993;
Summermatter et al., 1994). The type of SAR genes induced by salicylic
acid were also found to be induced by INA. In tobacco, coordinated induction of SAR genes by INA has been reported. These genes include those encoding PR-1 to PR-9 proteins plus a gene called SAR 8.2 (Ward et al., 1991).
In Arabidopsis, the same set of SAR genes and the spectrum of resistance
specificity were activated by salicylic acid and INA (Uknes et al., 1992,
1993).
Genetically engineered tobacco and Arabidopsis plants unable to accumulate salicylic acid failed to exhibit SAR after induction with pathogens
(Vernooij et al., 1994). However, application of INA still induced SAR and
SAR gene activity in such plants (Vernooij et al., 1994; Delaney et al.,
1995). INA did not induce salicylic acid glucoside accumulation in tobacco
(Malamy et al., 1996). However, INA was effective in inducing resistance
against P. syringae pv. tabaci in both wild-type Xanthi nc and transgenic
tobacco expressing salicylate hydroxylase (NahG) (Press et al., 1997), suggesting that INA action may not be mediated by salicylic acid.
INA may enter a common pathway downstream of salicylic acid synthesis (Malamy et al., 1996). This model is supported by the isolation and characterization of mutants in Arabidopsis that are insensitive to INA as well as
salicylic acid (Delaney et al., 1995). INA, as well as salicylic acid, inhibits
catalase and ascorbate peroxidase, two key enzymes for H2O2 degradation
(Durner and Klessig, 1995). INA induces at least some of the SAR genes induced by salicylic acid in different plants (Kogel et al., 1994) and also activates additional regulatory genes in addition to SAR genes (Siegrist et al.,
1994). INA was found to be a strong inducer of lipoxygenase (LOX) en-
zyme activity (Schaffrath et al., 1997; Schweizer et al., 1997). Levels of

jasmonic acid were enhanced in leaves of plants treated with INA (Schweizer et al., 1997). Hence, the octadecanoid pathway with ()-jasmonic acid as
a central component may be the INA-activated signaling pathway. However,
Schweizer and colleagues (1997) have presented evidences that INA may
induce the genes through another, uncharacterized signal transduction pathway. In rice, INA induced massive accumulation of an acid-soluble protein
of 31 kDa, ASP 31, which was not induced by salicylic acid, jasmonic acid,
ethylene, auxin, kinetin, abscisic acid, gibberellic acid, or by P. syringae pv.
syringae (Schweizer et al., 1997). ASP 31 appears to be a specifically INAinduced protein. Moreover, a number of in vitro translation products (ITPs),
ITP24, ITP25, ITP46, and ITP66, accumulated only in INA-treated rice
plants (Schweizer et al., 1997) and not in plants inoculated with P. syringae
pv. syringae, an SAR inducer (Smith and Metraux, 1991). Jasmonic acidinduced products did not accumulate in rice plants treated with INA (Schweizer et al., 1997). Exogenously applied jasmonic acid enhanced INA-induced
resistance and accumulation of PR-1 and ASP 31 in rice leaves. The fact that
ASP 31 accumulation is enhanced by jasmonic acid, although jasmonic acid
by itself could not induce accumulation of this protein, suggests the existence of a signaling network between the octadecanoid pathway and another
INA-induced pathway (Schweizer et al., 1997).
BTH
Benzo (1,2,3) thiadiazole-7-carbothioic acid S-methyl ester is another
plant activator that induces systemic resistance against various pathogens,
including fungi, bacteria, and viruses. In monocots, activated resistance by
BTH is very long lasting, while the lasting effect is less pronounced in dicots (Oostendorp et al., 2001). BTH is translocated systemically in plants
and has been shown to induce resistance in rice (Schaffrath et al., 1997),
wheat (Gorlach et al., 1996), barley (Besser et al., 2000), maize (Morris et al.,
1998), tobacco (Friedrich et al., 1996), cucumber (Benhamou and Belanger,
1998), Brassica (Jensen et al., 1998), and Arabidopsis (Lawton et al., 1996).
BTH induces systemic resistance in susceptible wheat to Blumeria graminis
f. sp. tritici (Stadnik and Buchenauer, 2000) and resistance against Beet necrotic yellow vein virus and Beet soilborne virus in beet (Research Information Ltd., 1998). BTH induced synthesis of at least nine acidic and six basic
proteins, including three acidic chitinase isozymes, three basic chitinases,
three acidic -1,3-glucanases, and one basic -1,3-glucanase in sugar beet
(Burketova et al., 1999). The signal transduction pathway induced by BTH
appears to be different from the pathway involved in SAR induced by in-
compatible pathogens. Genetically engineered tobacco and Arabidopsis

plants unable to accumulate salicylic acid failed to exhibit SAR after induction with pathogens. However, application of BTH still induced SAR and
SAR gene activity in such plants (Friedrich et al., 1996; Lawton et al.,
1996).
Schaffrath and colleagues (1997) inoculated wheat plants with an incompatible pathogen or sprayed them with a 1 mM BTH solution. Inoculation
with the nonhost pathogen resulted in accumulation of a set of wheat induced resistance (WIR) transcripts. WIR-1 and its homologue WIR-4 encode a putative cell wall protein (WIR-2), a thaumatin-like protein (PR-5
protein), and WIR-3, a peroxidase. BTH induced another set of transcripts
called wheat chemical induction (WCI) transcripts (Schaffrath et al., 1997).
WCI-4 encodes a cysteine proteinase, WCI-2 encodes lipoxygenase, and
WCI-1, WCI-3, and WCI-5 encode proteins of unknown function (Schaffrath et al., 1997). A nonhost pathogen did not induce WCI transcripts, while
BTH did not induce WIR transcripts. In wheat, the biological and chemical
(BTH) SAR inducers induce the accumulation of nonidentical sets of transcripts, indicating the existence of different signal transduction pathways.
Similar results have been obtained in rice. SAR induced by P. syringae
pv. syringae in rice results in the accumulation of transcripts homologous to
the wheat WIR-1, WIR-2, and WIR-3 clones such as PIR-1, PIR-2, and
PIR-3 (PIR denotes pathogen-induced protein from rice). BTH induced
SAR in rice. However, BTH treatment does not lead to the accumulation of
PIR transcripts in rice (Schaffrath et al., 1997). The types of signal transduction systems activated by BTH appear to be similar to that of INA
(Friedrich et al., 1996; Lawton et al., 1996; Schaffrath et al., 1997).
The compound acibenzolar-S-methyl (benzo (1,2,3) thiadiazole-7-carboxylic acid S-methyl ester, [BTH]) is available in Europe as Bion and in
the United States as Actigard. It reduces fire blight incidence (Momol,
Norelli, Aldwinckle, 1999; Zeller and Zeller, 1999). Control by acibenzolar-S-methyl (100 mg/liter) is usually equal to or better than streptomycin
(100 mg/liter) and induces resistance against bacterium. The protection of
apple seedlings was constantly associated with the activation of two families of defense-related enzymes, peroxidases and -1,3-glucanases (Brisset
et al., 2000). BTH plus streptomycin provides up to two times better control
than either BTH or streptomycin alone (Thomson et al., 1999). Acibenzolar-S-methyl applied as a soil drench or along with the marcot rooting
mixture induced resistance in sugarcane against the red rot pathogen,
Colletotrichum falcatum (Ramesh Sundar et al., 2001). The ISR persisted
up to 30 days in the pretreated canes. Increased phenolic content and accumulation of pathogenesis-related proteins, specifically PR-2, PR-3, and PR-5,
were observed in sugarcane plants treated with acibenzolar-S-methyl (Ramesh
Sundar et al., 2001). BTH reduced Potato virus Y infection in tobacco

(Burketova et al., 2000). Tobacco plants were sprayed with 1.2 mM BTH 4
days before inoculation with the virus, and BTH treatment decreased virus
multiplication in inoculated leaves. Activity of total ribonucleases and
biosynthesis of extracellular acid proteins were increased in both infected
and treated leaves. These results suggest that BTH can increase activity of
particular ribonuclease enzymes, which are induced by virus infection (Burketova et al., 2000).
Acibenzolar-S-methyl applied singly as pretransplant application or in
combination with the insecticide imidacloprid significantly reduced Tomato
spotted wilt virus (TSWV) incidence in flue-cured tobacco in Georgia
(Csinos et al., 2001). Initial applications of acibenzolar-S-methyl made
posttransplant did not control TSWV, suggesting plants must be protected
prior to introduction into the field. BTH induces resistance against Alternaria macrospora leaf spot of cotton (Colson-Hanks and Deverall, 2000). It
has also been reported that acibenzolar-S methyl induces resistance in cucumber against Colletotrichum lagenarium and Cladosporium cucumerinum, and in Japanese pear against Venturia nashicola (Ishii et al., 1999).
BABA
DL-3-aminobutyric acid ( -aminobutyric acid, BABA) induced systemic resistance against tomato late blight caused by Phytophthora infestans
(Jeun et al., 2000), grapevine downy mildew caused by Plasmopara viticola
(Cohen et al., 1999), pepper blight caused by Phytophthora capsici (Lee
et al., 2000), and tobacco downy mildew caused by Peronospora tabacina
(Cohen, 1994). It protected sunflower plants against Plasmopara helianthi
(= Plasmopara halstedii). BABA induced accumulation of free salicylic
acid in tomato leaves (Jeun et al., 2000). However, no enhancement of salicylic acid contents was found in the untreated upper leaves expressing systemic acquired resistance after treatment with BABA, suggesting that the
locally elevated salicylic acid might be necessary for the synthesis of the
still-unknown signal responsible for SAR expression (Jeun et al., 2000).
BABA also induces systemic resistance in tobacco (Cohen, 1994). Salicylic
acid and INA-treated tobacco plants showed accumulation of PR-1, -1,3glucanase, and chitinases. BABA did not induce these PR proteins, but all
three of them induced systemic resistance against Peronospora tabacina in
tobacco (Cohen, 1994). This evidence suggests that BABA induces ISR in a
pathway different from that induced by salicylic acid or INA.
BABA protected Arabidopsis against Peronospora parasitica through
activation of natural defense mechanisms of the plant, such as callose depo-
sition, the hypersensitive response, and the formation of trailing necroses

(Zimmerli et al., 2000). BABA was still protective against P. parasitica in
transgenic plants or mutants impaired in the salicylic acid, jasmonic acid,
and ethylene signaling pathways. BABA did not induce the accumulation of
mRNA of SAR-associated PR-1 and ethylene- and jasmonic acid-dependent PDF1.2 genes, suggesting that BABA may act through a pathway other
than the salicylic acid, jasmonic acid, and ethylene signaling pathways
(Zimmerli et al., 2000). It has been shown that BABA may potentiate the
plants to synthesize defense compounds only after pathogen attack (Zimmerli et al., 2000). BABA did not induce PR-1 mRNA but potentiated the
accumulation of PR-1 mRNA after attack by virulent pathogenic bacteria in
Arabidopsis (Zimmerli et al., 2000).
Salicylic Acid
Salicylic acid is an important signal molecule that plays a critical role in
plant defense against pathogen invasion. It induces resistance and reduces
disease incidence and is effective against fungal, bacterial, and viral diseases. It controls fungal pathogens Magnaporthe grisea, Fusarium oxysporum f. sp. lycopersici, and Diplodia pinea, the bacterial pathogen Xanthomonas oryzae pv. oryzae, and the virus Cucumber mosaic virus (Research
Information Ltd., 1998). The addition of 200 M of salicylic acid to the tomato root system significantly increased the endogenous salicylic acid content of leaves. Concomitant with elevated salicylic acid levels, expression of
the tomato pathogenesis-related PR-1B gene was strongly induced within
24 hours of the addition of 200 M of salicylic acid. PR-1B expression was
not observed in tomato plants not receiving salicylic acid treatment. Challenge inoculation of salicylic acid-treated tomato plants using conidia of
Alternaria solani resulted in 83 percent fewer lesions per leaf and a 77 percent reduction in blighted leaf areas compared to control plants not receiving salicylic acid (Spletzer and Enyedi, 1999). Salicylic acid protects barley
systemically against powdery mildew caused by Blumeria graminis f. sp.
hordei (Besser et al., 2000). The genes induced by the chemical were different from the genes induced during incompatible barley-Blumeria graminis
f. sp. hordei interactions governing gene-for-gene relationships, which suggests the presence of separate pathways leading to powdery mildew resistance (Besser et al., 2000).
Probenazole
Probenazole (3-allyloxy-1,2-benzisothiazole-1,1-dioxide) induces resistance against blast (Magnaporthe grisea) and bacterial blight (Xantho-
monas oryzae pv. oryzae) in rice (Oostendorp et al., 2001). Its commercial
name is Oryzemate. It induces several defense genes (Watanabe et al., 1979).
A gene, RPR1, was identified as a probenazole-responsive gene (Sakamoto
et al., 1999). RPR1 contains a nucleotide binding site and leucine-rich repeats, thus sharing structural similarity with known disease resistance
genes. The expression of RPR1 in rice could be up-regulated by treatment
with chemical inducers of SAR and by inoculation with pathogens. RPR1
was induced during the systemic induced resistance (Sakamoto et al., 1999).
Probenazole and its derivative BIT (1,2-benzisothiazol-3-(2H)-one 1,1-dioxide) induced resistance against Pseudomonas syringae pv. tomato and
Peronospora parasitica in A. thaliana (Yoshioka et al., 2001). They induced
expression of several pathogenesis-related genes and accumulation of salicylic acid in A. thaliana (Yoshioka et al., 2001). Probenazole or BIT treatment did not induce disease resistance or PR-1 expression in NahG transgenic plants (salicylic acid is degraded in these plants) or npr1 mutant A.
thaliana plants (npr1 is a key component in the salicylic acid signaling pathway). However, probenazole or BIT treatment induced disease resistance
and PR-1 expression in Arabidopsis etr1-1 mutant plants, which are insensitive to ethylene, and coi1-1 mutant plants, which are insensitive to methyl
jasmonate (Yoshioka et al., 2001). These results suggest that the salicylic
acid pathway is required for probenazole-mediated activation of defense responses, while signal transduction pathways involving ethylene or methyl
jasmonate are not required. Probenazole may stimulate the salicylic acid/
NPR1-mediated defense signaling pathway upstream of salicylic acid (Yoshioka et al., 2001).
Prohexadione-Ca
The plant growth regulator prohexadione-Ca acts as a plant activator. It
has no direct effect on growth of E. amylovora, the apple fire blight pathogen. However, it reduced the incidence of fire blight and causes major
changes in the flavonoid metabolism of apple. Within four hours after treatment, a new flavonoid (identified as luteoliflavan) occurs in leaf and flower
tissue (Roemmelt, Truetter, et al., 1999). Apple trees treated with 250 mg/liter prohexadione-Ca late in the bloom period gave a significantly lower incidence of secondary shoot blight (caused by E. amylovora) than unsprayed
trees (Fernando and Jones, 1999). Prohexadione-Ca was more effective than
Actigard in controlling the apple shoot blight (Momol, Ugine, et al., 1999).
Aluminium Chloride (Synermix)

Hexahydratedaluminium chloride (AlCl3) is the constituent of the new
fungicide Synermix (sold by Laboratoires Goemar of France). It acts as a
plant activator, activating synthesis of phytoalexins in grapevines. Synermix, when applied in combination with iprodione, increases the efficiency
of iprodione in controlling Botrytis cinerea in grapevine (Jeandet et al.,
2000).
Riboflavin
Riboflavin is a vitamin produced by plants and microbes. Foliar application of riboflavin induced systemic resistance against Peronospora parasitica and Pseudomonas syringae pv. tomato in Arabidopsis thaliana, and
against Tobacco mosaic virus and Alternaria alternata in tobacco (Dong
and Beer, 2000). It reduces powdery mildew of strawberry plants (Wang and
Tzeng, 1998). Riboflavin is involved in antioxidation and peroxidation, processes that affect the production of oxidative burst. The reaction involves
complex redox processes that require participation by specific signal molecules, such as H2O2 and nitric oxide, and antioxidants, such as tocopherols
and riboflavin (for review, see Dong and Beer, 2000). It acts as plant activator. Riboflavin induced expression of pathogenesis-related genes in tobacco
and Arabidopsis plants (Dong and Beer, 2000). The response to riboflavin is
negated by the protein kinase inhibitor K252a and by mutation of the
NIM1/NPR1 gene (NIM1/NPR1 encodes a protein that controls expression
of various sets of defense genes), but is unaffected in NahG plants (Dong
and Beer, 2000). NahG plants expressing the bacterial nahG gene, which
encodes salicylate hydroxylase that degrades salicylic acid, are incapable of
accumulating sufficient salicylic acid to induce systemic acquired resistance when challenged with pathogens. These findings suggest that riboflavin triggers signals that require protein kinases and regulation by NIM1/
NPR1 gene, but does not require accumulation of salicylic acid.
KeyPlex
KeyPlex 350 is a micronutrient preparation. It is a plant activator, activating the plant defense system. Formulations based on KeyPlex 350, applied
at 1.90 liters/ 378.53 liters of water per hectare, effectively controlled Sigatoka leaf spot caused by Mycosphaerella musicola in Florida (McMillan
et al., 2000).
Myco-Sin and Ulmasud

Two products based on mineral powders, Myco-Sin and Ulmasud, reduced apple blossom infections caused by Erwinia amylovora in Germany.
These products induced synthesis of phenolic acids which resulted in induced
resistance (Roemmelt, Plagge, et al., 1999).
Carpropamid
Carpropamid is a new systemic fungicide effective against the rice blast
caused by Pyricularia oryzae. It induced lignification of infected rice cells
and increased accumulation of the phytoalexins momilactone A and sakuranetin in P. oryzae-infected leaves (Thieron et al., 1998; Araki and Kurahashi, 1999). It induced resistance against the blast pathogen (Thieron et al.,
1998).
Phytogard (K2HPO3 )
Phytogard is a formulation containing 58 percent potassium phosphonate
(Becot et al., 2000). It induces resistance against downy mildew of cauliflower (Brassica oleracea var. botrytis) caused by Peronospora parasitica.
It provided complete protection of seedlings, when sprayed with Phytogard
at 7 mL/liter of water. The induced resistance persisted for at least 15 days.
-1,3-glucanase activity and PR-2 proteins were induced (Becot et al.,
2000).
Phosphate
Phosphorous-containing nutrient solutions of 20 ppm, applied through a
hydroponics system, provided ISR against the powdery mildew pathogen
Sphaerotheca fuliginea in cucumber seedlings (Reuveni et al., 2000). Foliar
application of 1 percent solution of monopotassium phosphate effectively
protected the foliage against powdery mildew. This treatment was persistent
up to 21 days after inoculation. It significantly inhibited powdery mildew
development and caused a reduction of 72 percent in sporulation of the fungus compared to control (Reuveni et al., 2000).
Methyl Jasmonate/Jasmonic Acid
Jasmonic acid induces systemic resistance against many pathogens. It
controls Botrytis cinerea in different crops (Research Information Ltd.,
1998). Jasmonates protect barley plants against Blumeria graminis f. sp.

hordei (Besser et al., 2000) by strengthening plant defense mechanisms that
result in effective papillae and host cell death (Besser et al., 2000).
PRACTICAL USES OF PLANT ACTIVATORS
Almost three decades have passed since the effects of salicylic acid and
other chemicals inducing disease resistance were first reported. However,
large-scale field use of these chemicals has not yet been reported. The major
drawback of the chemical induction of defense genes is that their effect is
only transient; the induced resistance by chemicals lasts for only a few days.
Methods of application such as infiltration by injection or root feeding are
impractical under field conditions. When these chemicals are sprayed on foliage, they are easily washed away by rain or dew. They should be applied
before infection, as they sensitize the plants for induction of defense genes
during the infection process. They are not curative and cannot eliminate already established infection. Most of the chemicals are phytotoxic. However,
recently identified chemicals such as INA and BTH persist for a long time,
from 30 to 45 days. They can be sprayed or applied through the soil. It appears that these two chemicals have the potential for large-scale field use in
management of diseases. The time of application, dosage required, and
mode of application must be standardized against each disease (Vidhyasekaran, 2002).
INA and BTH induce resistance by activating genes that are not normally
activated during SAR induced by biological agents, which suggests many
other defense genes are not activated by INA or BTH. Hence, these chemicals alone cannot contribute to high resistance. In other words, they can only
reduce disease intensity and cannot completely control disease (Press et al.,
1997). These chemicals should be one of the treatments and not the only
treatment to control diseases. For example, efficacy of INA was enhanced
with additional application of jasmonic acid in rice (Schweizer et al., 1997).
These chemicals may be useful in moderately disease-resistant cultivars
rather than in susceptible cultivars and/or when they are combined with
other chemicals.
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29
Plant
PlantExtracts
Extracts
Several plant extracts have been shown to control diseases. Some of them
have been developed as commercial formulations. Plant extracts may have
antimicrobial action, or they may induce disease resistance by activating a
signal transduction system in plants. The possible uses of plant extracts in
crop disease management are described in this chapter.
ANTIMICROBIAL ACTION OF PLANT EXTRACTS
Several plant extracts have been reported to possess antimicrobial action.
Proteins from the seeds of 200 Australian native plant accessions were extracted, and most of them were found to show antimicrobial activity. The
peptides isolated from Hardenbergia violacea and Macadamia integrifolia
showed strong inhibitory activity in vitro toward plant pathogens (Harrison
et al., 1998). A novel antifungal compound, fistulosin (octadecyl 3-hydroxyindole), isolated from roots of Welsh onion (Allium fistulosum) showed high
activity against Fusarium oxysporum, primarily inhibiting protein synthesis
(Phay et al., 1999). Several articles have appeared in recent years describing
antimicrobial compounds isolated from various plants. However, only very
few of these compounds have been tested for crop-disease management under field conditions. Leaf extracts of Annona cherimola, Bromelia hemisphaerica, and Carica papaya inhibited sporulation of Rhizopus stolonifer,
the pathogen of circula fruit (Spondias purpurea) rot. They also reduced
fruit rot infection and development (Bautista-Banos et al., 2000). Garlic
juice and grapefruit juice (BioSept) effectively controlled rose powdery
mildew (Sphaerotheca pannosa var. rosae), and their efficacy was equal to
that of the standard fungicide triforine (Wojdyla, 2000). When root extracts
of Mirabilis jalapa, containing a ribosome inactivating protein called Mirabilis antiviral protein (MAP), were sprayed on potato plants, infection by
Potato virus X (PVX) and potato spindle tuber viroid was inhibited by almost 100 percent (Vivanco et al., 1999). Similar results were obtained in tomato plants against PVX infection. Antiviral activity of MAP extracts was
observed against mechanically transmitted viruses, but the extracts did not
show activity against aphid transmitted viruses, such as Potato virus Y

(PVY) and Potato leaf roll virus (PLRV) on potatoes (Vivanco et al., 1999).
Commercial formulations of various plant extracts, such as pepper/mustard (chilli pepper extract and the essential oil of mustard), cassia (extract of
cassia tree), clover (70 percent clove oil), and neem (90 percent neem oil)
were tested for their efficacy to control muskmelon wilt caused by Fusarium oxysporum f. sp. melonis (Bowers and Locke, 2000). Soil infested with
F. oxysporum f. sp. melonis was treated with 5 to 10 percent aqueous emulsions of commercial formulations of these plant extracts, and these treatments,
excepting neem oil, effectively controlled the wilt disease of muskmelon in
the greenhouse. Pepper/mustard, cassia, and clove extracts suppressed disease development (80 to 100 percent healthy plant stand) compared to the
untreated infested soil (< 20 percent stand). Neem oil extract was ineffective
against Fusarium spp. (Bowers and Locke, 2000).
INDUCTION OF DISEASE RESISTANCE
BY PLANT EXTRACTS
Several plant extracts induced resistance against diseases by activating
signal transduction systems. Milsana, a commercial product of leaf extracts
from the giant knot weed Reynoutria sachalinensis, significantly reduced
the incidence of cucumber (Cucumis sativus) powdery mildew (Sphaerotheca fuliginea) (Wurms et al., 1999; Daayf et al., 2000). Milsana was more
effective than the plant activator benzothiadiazole (BTH) in controlling
powdery mildew on long English cucumber cv. Corona (Wurms et al.,
1999). Milsana application induced localized resistance. Most haustoria of
the pathogen had collapsed in the localized Milsana treatment and were
encapsulated by an amorphous material impregnated by electron-opaque
substances, probably phenolic substances (Wurms et al., 1999). Milsana
treatment increased the levels of p-coumaric, caffeic, and ferulic acids and
p-coumaric acid methyl ester in the cucumber leaves. The amounts of
hydroxycinnamic acid increased rapidly in the two test cultivars of cucumber following Milsana treatment (Daayf et al., 2000). All of these phenolic
compounds showed antifungal activity against common pathogens of cucumber, such as Botrytis cinerea, Pythium ultimum, and P. aphanidermatum, which suggests that the plant extract would induce synthesis of
antifungal compounds and contribute to disease resistance (Daayf et al.,
2000).
Pokeweed (Phytolacca spp.) extract has been shown to inhibit Tobacco
mosaic virus, Potato virus X, Potato virus Y, Cucumber mosaic virus, Southern bean mosaic virus, and Alfalfa mosaic virus (Baranwal and Verma,
2000). Several antiviral proteins have been isolated from pokeweed extract.
These proteins show ribosome inhibitory action against viruses. A pokeweed antiviral protein (PAP), a 29 kDa protein isolated from Phytolacca
americana, inhibits translation by catalytically removing a specific adenine
residue from the large rRNA of the 60S subunit of eukaryotic ribosomes
(Smirnov et al., 1997). The PAP depurinates the sarcin/ricin (S/R) loop of
the large rRNA of prokaryotic and eukaryotic ribosomes (Zoubenko et al.,
2000). A mutant of pokeweed antiviral protein, PAPn, which has a single
amino acid substitution (Gly-75), did not bind ribosomes efficiently, indicating Gly-75 in the N-terminal domain is critical for the binding of PAP to
ribosomes. PAPn did not depurinate ribosomes and was nontoxic when expressed in transgenic tobacco plants. The transgenic plants, which expressed
nontoxic protein, were also resistant to viral and fungal infection (Zoubenko
et al., 2000). These results suggest that PAP may act as inducer of systemic
resistance against viruses, rather than by directly inhibiting viruses. PAP induces synthesis of pathogenesis-related proteins and a small increase in salicylic acid levels in tobacco. An intact active site of PAP is necessary for induction of virus resistance in plants. Enzymatic activity of PAP appears to
be responsible for generating a signal that renders tobacco plants resistant to
virus infection (Smirnov et al., 1997).
Plant extracts have the potential to control various diseases. However,
they are less stable on sprayed plants, and frequent application of these
products is needed to manage diseases efficiently. Further research is necessary to obtain highly stable compounds.
REFERENCES
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agents for efficient management of plant viruses. In R. K. Upadhyay, K. G.
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(2000). Evaluation of the fungicidal properties of plant extracts to reduce
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Daayf, F., Ongena, M., Boulanger, R., El-Hadrami, I., and Belanger, R. R. (2000).
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Harrison, S. J., Marcus, J. P., Green, J. L., Goulter, K. C., Maclean, D. J., and Manners, J. M. (1998). Isolation and characterization of antimicrobial proteins from
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Tomita, F. (1999). An antifungal compound from roots of Welsh onion. Phytochemistry, 52:271-274.
Smirnov, S., Shulaev, V., and Tumer, N. E. (1997). Expression of pokeweed antiviral protein in transgenic plants induced virus resistance in grafted wild-type
plants independently of salicylic acid accumulation and pathogenesis-related
protein synthesis. Plant Physiol, 114:1113-1121.
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Wojdyla, A. T. (2000). Influence of some compounds on development of Sphaerotheca pannosa var. rosae. J Plant Protect Res, 40:106-121.
Wurms, K., Labbe, C., Benhamou, N., and Belanger, R. R. (1999). Effects of
Milsana and benzothiadiazole on the ultrastructure of powdery mildew haustoria
on cucumber. Phytopathology, 89:728-736.
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protein mutant inhibits pathogen infection via a novel salicylic acid-independent
pathway. Plant Mol Biol, 44:219-229.
CROP DISEASES
30
Diseases
Diseases
of of
Major
Major Cultivated
Cultivated
CropsCrops
In the following sections, a comprehensive list of diseases occurring in
major crops grown in different parts of the world is presented. The common
names of the diseases given in these sections are the American Phytopathological Society approved names (Hansen, 1985, 1988, 1991; APSnet,
1997). Names of fungal pathogens (their accepted names, synonyms, anamorphic and teleomorphic names) with authority are given (Hawksworth
et al., 1995; Wrobel and Creber, 1998). Synonyms are given as =. Names of
the bacterial pathogens are given based on papers published by Young and
colleagues (1996) and Schaad and colleagues (2000). The names include
accepted names and all valid synonyms, which are indicated as =. Names of
viruses have been given based on the guidelines provided by Van Regenmortel (1999).
APPLE (MALUS
DOMESTICA BORKH.)
Alternaria blotch: Alternaria mali Roberts = A. alternata (Fr.:Fr) Keissl,

apple pathotype
American brown rot: Monilinia fructicola (G. Wint.) Honey
American hawthorne rust: Gymnosporangium globosum (Farl.) Farl.
Anthracnose canker: Pezicula malicorticis (H. Jacks.) Nannf, Anamorph:
Cryptosporiopsis curvispora (Peck) Gremmen in Boerema and
Gremmen
Apple blister bark: Apple fruit crinkle viroid (AFCVd)
Apple chlorotic leafspot: genus Trichovirus, Apple chlorotic leaf spot
virus (ACLSV)
Apple dimple fruit: Apple scar skin viroid (ASSVd)
Apple flat apple: genus Nepovirus, Cherry rasp leaf virus (CRLV) (family
Comoviridae)
Apple fruit crinkle: Apple fruit crinkle viroid (AFCVd)
Apple mosaic: genus Ilarvirus, Apple mosaic virus (ApMV); genus
Ilarvirus, Tulare apple mosaic virus (TAMV)
Apple proliferation: Phytoplasma
Apple ring rot and canker: Botryosphaeria berengeriana De Not. =

Physalospora piricola Nose
Apple scar skin: Apple scar skin viroid (ASSVd)
Apple stem grooving: genus Capillovirus, Apple stem grooving virus
(ASGV)
Apple stem pitting: genus Foveavirus, Apple stem pitting virus (ASPV)
Apple union necrosis: genus Nepovirus, Tomato ringspot virus (ToRSV)
(family Comoviridae)
Armillaria root rot: Armillaria mellea (Vahl:Fr.) P. Kumm.
Bitter rot: Glomerella cingulata (Stoneman) Spauld. and H. Schrenk,
Anamorph: Colletotrichum gloeosporioides (Penz.) Penz. and Sacc. in
Penz., Colletotrichum acutatum J. H. Simmons
Black pox: Helminthosporium papulosum Berg.
Black root rot: Xylaria mali Fromme, X. polymorpha (Pers.:Fr.) Grev.
Black rot and stem canker: Botryosphaeria obtusa (Schwein.) Shoemaker,
Anamorph: Sphaeropsis malorum Berk. (Mitosporic fungi) =
Physalospora obtusa (Schwein) Cooke
Blister canker: Biscogniauxia marginata (Fr.) Pouzar = Nummularia
discreta (Schwein.) Tul. and C. Tul.
Blister spot: Pseudomonas syringae pv. papulans (Rose 1917)
Dhanvantari 1977
Blue mold: Penicillium spp., P. expansum Link
Brooks fruit spot: Mycosphaerella pomi (Pass.) Lindau, Anamorph:
Cylindrosporium pomi C. Brooks
Brown rot: Monilinia laxa (Aderhold and Ruhland) Honey
Calyx-end rot: Sclerotinia sclerotiorum (Lib.) de Bary
Cedar apple rust: Gymnosporangium juniperi-virginianae Schwein
Clitocybe root rot: Armillaria tabescens (Scop.) Dennis et al. = Clitocybe
tabescens (Scop.) Bres.
Crown gall: Agrobacterium tumefaciens (Smith and Townsend 1907)
Conn 1942
Diaporthe canker: Diaporthe tanakae Kobayashi and Sakuma,
Anamorph: Phomopsis tanakae Kobayashi and Sakuma
Diplodia canker: Botryosphaeria stevensii Shoemaker = Physalospora
malorum Shear et al., Anamorph: Diplodia mutila (Fr.:Fr.) Mont.
European brown rot: Monilinia fructigena Honey in Whetzel,
Anamorph: Monilia fructigena Pers.:Fr., Monilinia laxa (Aderhold and
Ruhland) Honey
Fire blight: Erwinia amylovora (Burrill 1882) Winslow, Buchanan,
Krumwiede, Rogers, and Smith, 1920
Fisheye rot: Butlerelfia eustacei Weresub and Illman = Corticium
centrifugum (Lv.) Bres.
Fly speck: Schizothyrium pomi (Mont.:Fr.) Arx, Anamorph: Zygophiala

jamaicensis E. Mason
Gray mold rot: Botrytis cinerea Pers. Fr., Teleomorph: Botryotinia
fuckeliana (de Bary) Whetzel
Hairy root: Agrobacterium rhizogenes (Riker, Banfield, Wright, Keitt, and
Sagen 1930) Conn 1942
Japanese apple rust: Gymnosporangium yamadae Miyabe ex Yamada
Leptosphaeria canker: Diapleella coniothyrium (Fuckel) Barr =
Leptosphaeria coniothyrium (Fuckel) Sacc., Anamorph: Coniothyrium
fuckelii Sacc.
Leucostoma canker: Leucostoma cincta (Fr.:Fr.) Hohn., Anamorph:
Cytospora cincta Sacc., Valsa auerswaldii Nitschke = Leucostoma
auerswaldii (Nitschke) Hohn.
Marssonina blotch: Diplocarpon mali Harada and Sawamura, Anamorph:
Marssonina coronaria (Ellis and J. J. Davis) J. J. Davis
Monilia leaf blight: Monilinia mali (Takahashi) Whetzel, Anamorph:
Monilia sp.
Monochaetia twig canker: Monochaetia mali (Ellis and Everh.) Sacc.,
Teleomorph: Lepteutypa cupressi (Nattras et al.) H. J. Swart
Mucor rot: Mucor spp., Mucor piriformis E. Fischer
Nectria canker: Nectria galligena Bres. in Strass., Anamorph:
Cylindrocarpon heteronemum (Berk. and Broome) Wollenweb
Nectria twig blight: Nectria cinnabarina (Tode:Fr.) Fr., Anamorph:
Tubercularia vulgaris Tode:Fr.
Pacific Coast pear rust: Gymnosporangium libocedri (C. Henn.) F. Kern
Peniophora root canker: Peniophora sacrata G. H. Cunn.
Perennial canker: Neofabrae perennans Kienholz, Anamorph:
Cryptosporiopsis perennans (Zeller and Childs) Wollenweb.
Phomopsis canker: Phomopsis mali Roberts, Teleomorph: Diaporthe
perniciosa Em. Marchal
Phymatotrichum root rot: Phymatotrichopsis omnivora (Duggar)
Hennebert = Phymatotrichum omnivorum Duggar
Phytophthora crown and root rot: Phytopthora cactorum (Lebert and
Cohn) J. Schrt., P. cambivora (Petr.) Buisman, P. cryptogea Pethybr.
and Lafferty, P. megasperma Drechs., P. syringae (Kleb.) Kleb.
Pink mold rot: Trichothecium roseum (Pers.:Fr.) Link = Cephalothecium
roseum Corda
Powdery mildew: Podosphaera leucotricha (Ellis and Everh.) E. S.
Salmon
Quince rust: Gymnosporangium clavipes (Cooke and Peck) Cooke and
Peck in Peck
Rosellinia root rot: Rosellinia necatrix Prill., Anamorph: Dematophora

necatrix R. Hartig
Scab: Venturia inaequalis (Cooke) G. Wint., Anamorph: Spilocaea pomi
Fr.:Fr.
Side rot: Phialophora malorum (M. N. Kidd and A. Beaumont)
McColloch
Silver leaf: Chondrostereum purpureum (Pers.:Fr.) Pouzar
Sooty blotch complex: Peltaster fructicola Johnson, Sutton, and Hodges,
Geastrumia polystigmatis Batista and M. L. Farr, Leptodontium elatius
(G. Mangenot) De Hoog, Gloeodes pomigena (Schwein.) Colby
Southern blight: Sclerotium rolfsii Sacc., Teleomorph: Athelia rolfsii
(Curzi) Tu and Kimbrough
Thread blight: Corticium stevensii Burt = Pellicularia koleroga Cooke =
Hypochnus ochroleucus Noack
Twig canker: Phyllosticta solitaria Ellis and Everh.
Valsa canker: Valsa ceratosperma (Tode:Fr.), Anamorph: Cytospora
sacculus (Schwein.) Gvritischvili
Violet root rot: Helicobasidium mompa Tanaka
White root rot: Scytinostroma galactinum (Fr.) Donk = Corticium
galactinum (Fr.) Burt
White rot and stem canker: Botryosphaeria dothidea (Moug.) Ces.
and De Not., Anamorph: Fusicoccum aesculi Corda
X-spot (Nigrospora spot): Nigrospora oryzae (Berk. and Broome) Petch
Zonate leaf spot: Cristulariella moricola (Hino) Redhead, Teleomorph:
Grovesinia pyramidalis M. Cline et al.
BANANA (MUSA SPECIES)
Anthracnose: Colletotrichum musae (Berk. and M. A. Curtis) Arx
Armillaria rot: Armillaria mellea (Vahl:Fr.) P. Kumm., Armillaria
tabescens (Scop.) Dennis, Orton, and Hora
Black cross: Phyllachora musicola C. Booth and D. E. Shaw
Black root rot: Rosellinia bunodes (Berk. and Broome) Sacc.
Black Sigatoka: Mycosphaerella fijiensis M. Morelet, Anamorph:
Paracercospora fijiensis (M. Morelet) Deighton
Bract mosaic: genus Potyvirus, Banana bract mosaic virus (BBMV)
(family Potyviridae)
Brown blotch: Pestalotiopsis leprogena (Speg.) Steyaert
Brown spot: Cercospora hayi Calp.
Bunchy top: genus Nanavirus, Banana bunchy top virus (BBTV)
Ceratocystis fruit rot: Ceratocystis paradoxa (Dade) C. Moreau,

Anamorph: Chalara paradoxa (De Seynes) Sacc.
Cigar-end: Verticillium theobromae (Turconi) E. W. Mason and S. J.
Hughes
Cladosporium speckle: Cladosporium musae E. W Mason
Cordana leaf spot: Cordana johnstonii M. B. Ellis, Cordana musae
(Zimm.) Hhn
Corm dry rot: Junghuhnia vincta (Berk.) Hood and M. Dick
Deightoniella fruit speckle and leaf spot: Deightoniella torulosa (Syd.)
M. B. Ellis
Diamond spot: Cercospora hayi Calp.
Dwarf Cavendish tip rot: Nattrassia mangiferae (Syd. and P. Syd.)
B. Sutton and Dyko = Hendersonula toruloidea Nattrass
Eyespot: Dreschslera gigantea (Heald and F. A. Wolf) S. Ito
Fruit freckle: Guignardia musae Racib., Anamorph: Phyllosticta
musarum (Cooke) Aa
Fruit rot: Botryosphaeria ribis Grossenb. and Duggar
Fungal root-rot: Fusarium solani (Mart.) Sacc., Teleomorph: Nectria
haematococca Berk. and Broome, Fusarium oxysporum Schltdl.:Fr.,
and Rhizoctonia spp.
Fungal scald: Colletotrichum musae (Berk. and M. A. Curtis) Arx.
Fusarium wilt (Panama disease): Fusarium oxysporum Schltdl.:Fr. f. sp.
cubense (E. F. Sm.) W. C. Snyder and H. N. Hansen
Leaf rust: Uredo musae Cummins, Uromyces musae Henn.
Leaf speckle: Acrodontium simplex (G. Mang.) de Hoog
Leaf spots: Curvularia eragrostidis (Henn.) J. A. Mey., Drechslera
musae-sapientum Hansf. and M. B. Ellis, Leptosphaeria musarum
Sacc. and Berl., Pestalotiopsis disseminata (Thm.) Steyaert
Main stalk rot: Ceratocystis paradoxa (Dade) C. Moreau
Malayan leaf spot: Haplobasidion musae M. B. Ellis
Marasmiellus rot: Marasmiellus inoderma (Berk.) Singer = Marasmius
semiustus Berk. and M. A. Curt.
Moko, bacterial wilt: Ralstonia solanacearum (Smith 1896) Yabauuchi,
Kosako, Yano, Hotta, and Nishiuchi 1995 = Burkholderia
solanacearum (Smith 1896) Yabuuchi, Kosako, Oyaizu, Yano, Hotta,
Hashimoto, Ezaki, and Arakawa 1993 = Pseudomonas solanacearum
(Smith 1896) Smith 1914
Mosaic: genus Cucumovirus, Cucumber mosaic virus (CMV) (family
Bromoviridae)
Pestalotiopsis leaf spot: Pestalotiopsis palmarum (Cooke) Steyaert
Phaeoseptoria leaf spot: Phaeoseptoria musae Punith.
Pitting: Pyricularia grisea Sacc.
Pseudostem heart rot: Fusarium moniliforme J. Sheld., Teleomorph:

Gibberella fujikuroi (Sawada) S. Ito in S. Ito and K. Kimura
Rhizome rot: Erwinia carotovora (Jones 1901) Bergey, Harrison, Breed,
Hammer, and Huntoon 1923, Erwinia chrysanthemi Buckholder,
McFadden, and Dimock 1953
Root and rhizome rot: Cylindrocarpon musae C. Booth and R. H. Stover
Sclerotinia fruit rot: Sclerotinia sclerotiorum (Lib.) de Bary
Sheath rot: Nectria foliicola Berk. and M. A. Curtis
Sooty mold: Limacinula tenuis (Earle) Sacc. and Trotter in Sacc.
Speckle: Mycosphaerella musae (Speg.) Syd. and P. Syd.
Squirter (black end disease): Nigrospora sphaerica (Sacc.) E. W. Mason
Stem-end rot: Colletotrichum musae (Berk. and M. A. Curtis) Arx
Streak: genus Badnavirus, Banana streak virus (BStV)
Trachysphaera finger rot: Trachysphaera frutigena Tabor and Bunting
Tropical speckle: Ramichloridium musae (Stahel ex M. B. Ellis) De Hoog
= Veronaea musae Stahel ex M. B. Ellis = Periconiella musae Stahel ex
M. B. Ellis
Verticillium tip rot: Verticillium theobromae (Turconi) E. W. Mason and
S. J. Hughes
Yellow Sigatoka: Mycosphaerella musicola J. L. Mulder in J. L. Mulder
and R. H. Stover, Anamorph: Pseudocercospora musae (Zimm.)
Deighton
BARLEY (HORDEUM VULGARE L. EMEND. BOWDEN)
Anthracnose: Colletotrichum graminicola (Ces.) G. W. Wils.,
Teleomorph: Glomerella graminicola Politis
Aster yellows: Aster yellows phytoplasma
Bacterial kernel blight, leaf blight: Pseudomonas syringae pv. syringae
van Hall 1902
Bacterial stripe: Pseudomonas syringae pv. striafaciens (Elliott 1927)
Barley mild mosaic: genus Bymovirus, Barley mild mosaic virus
(BaMMV) (family Potyviridae)
Barley stripe: Drechslera graminea (Rabenh.) Shoemaker
Barley stripe mosaic: genus Hordeivirus, Barley stripe mosaic virus
(BSMV)
(BYDV)
Barley yellow mosaic: genus Bymovirus, Barley yellow mosaic virus
(BaYMV) (family Potyviridae)
Basal glume rot: Pseudomonas syringae pv. atrofaciens (McCulloch

1920) Young, Dye, and Wilkie 1978
Black chaff and bacterial streak: Xanthomonas translucens pv.
translucens (Jones, Johnson, and Reddy 1917) Vauterin, Hoste,
Kersters, and Swings 1995
Brome mosaic: genus Bromovirus, Brome mosaic virus (BMV)
Cephalosporium stripe: Hymenula cerealis Ellis and Everh. =
Cephalosporium gramineum Nisikado and Ikata in Nisikado et al.
Cereal northern mosaic: genus Cytorhabdovirus, Northern cereal mosaic
virus (NCMV) (family Rhabdoviridae, Order Mononegavirales)
Cereal tillering: genus Reovirus, Cereal tillering disease virus (CTDV)
Chloris striate mosaic: genus Monogeminivirus, Chloris striate mosaic
virus (CSMV)
Covered smut: Ustilago hordei (Pers.) Lagerh.
Crown rot: Fusarium culmorum (Wm. G. Smith) Sacc., F. graminearum
Schwabe, Teleomorph: Gibberella zeae (Schwein.) Petch, Bipolaris
sorokiniana (Sacc.) Shoemaker
Crown rust: Puccinia coronata Corda
Downy mildew: Sclerophthora rayssiae Kenneth et al.
Dwarf bunt: Tilletia controversa Kuhn in Rebenh.
Ergot: Claviceps purpurea (Fr.:Fr.) Tul., Anamorph: Sphacelia segetum
Lev.
Eyespot: Pseudocercosoporella herpotrichoides (Fron) Deighton,
Teleomorph: Tapesia yallundae Wallwork and Spooner
False loose smut: Ustilago avenae (Pers.) Rostr
Gray snow mold (= Typhula blight): Typhula incarnata Fr., T.
ishikariensis Imai
Halo spot: Pseudoseptoria donacis (Pass.) Sutton = Selenophoma donacis
(Pass.) R. Sprague and A. G. Johnson
Hordeum mosaic: genus Rymovirus, Hordeum mosaic virus (HoMV)
(family Potyviridae)
Leaf rust (= brown rust): Puccinia hordei Otth
Leaf spot: Ascochyta hordei K. Hara, Ascochyta graminea (Sacc.)
R. Sprague and A. G. Johnson, Ascochyta sorghi Sacc., Ascochyta
tritici S. Hori and Enjoji
Loose smut: Ustilago segetum (Pers.) Roussel var. nuda = Ustilago nuda
(Jensen) Rostr.
Net blotch: Pyrenophora teres Drechs., Anamorph: Drechslera teres
(Sacc.) Shoemaker = Helminthosporium teres Sacc., Drechslera teres f.
maculata Smedeg.
Oat blue dwarf: genus Marafivirus, Oat blue dwarf virus (OBDV)
Oat pseudorosette: genus Tenuivirus, Oat pseudorosette virus
Oat sterile dwarf: genus Fijivirus, Oat sterile dwarf virus (OSDV)
Pink snow mold: Microdochium nivalis (Fr.) Samuel and I. C. Hallett =
Fusarium nivale (Fr.) Sorauer, Teleomorph: Monographella nivalis
(Schaffnit) E. Mller
Powdery mildew: Blumeria graminis (DC.) E. O. Speer f. sp. hordei Em.
Marchel, Anamorph: Oidium monilioides (Nees) Link = Erysiphe
graminis DC, ex Merat f. sp. hordei Em. Marchal
Pythium root rot: Pythium spp., Pythium arrhenomanes Drechs., Pythium
graminicola Subramanian, Pythium tardicrescens Vanderpool
Rhizoctonia root rot: Rhizoctonia solani Kuhn, Teleomorph:
Thanatephorus cucumeris (A. B. Frank) Donk
Rice black-streaked dwarf: genus Fijivirus, Rice black-streaked dwarf
virus (RBSDV)
Rice stripe: genus Tenuivirus, Rice stripe virus (RSV)
Scab (= head blight): F. graminearum Schwabe
Scald: Rhynchosporium secalis (Oud.) J. J. Davis
Septoria speckled leaf blotch: Septoria passerinni Sacc., Stagonospora
avenae f. sp. triticea T. Johnson
Sharp eyespot: Rhizoctonia cerealis Van der Hoeven, Teleomorph:
Ceratobasidium cereale D. Murray and L. L. Burpee
Snow rot: Pythium iwayamai Ito, P. okanoganense Lipps, P. paddicum
Harane
Snow scald: Myriosclerotinia borealis (Bubak and Vleugel) L. M. Kohn =
Sclerotinia borealis Bubak and Vleugel
Speckled snow mold: Typhula idahoensis Remsberg
Spot blotch: Cochliobolus sativus (Ito and Kuribayashi) Drechs. ex Dastur,
Anamorph: Drechslera teres (Sacc.) Shoemaker
Stagonospora blotch: Stagonospora avenae f. sp. triticea T. Johnson,
Teleomorph: Phaeosphaeria avenaria f. sp. triticea T. Johnson,
Stagonospora nodorum (Berk.) Castellani and E. G. Germano =
Septoria nodorum (Berk.) Berk. in Berk. and Broome, Teleomorph:
Phaeosphaeria nodorum (E. Muller) Hedjaroude
Stem rust (= black stem rust): Puccinia graminis Pers.:Pers. = Puccinia
graminis f.sp. tritici (Pers.) Erikss. et Henn
Take-all: Gaeumannomyces graminis (Sacc.) von Arx and Olivier var.
tritici Walker = Ophiobolus graminis Sacc.
Tan spot: Pyrenophora tritici-repentis (Died.) Drechs. = P. trichostoma
(Fr.) Fuckel, Anamorph: Drechslera tritici-repentis (Died.) Shoemaker
= Helminthosporium tritici-repentis Died.
Verticillium wilt: Verticillium dahliae Kleb.
Wheat dwarf: genus Monogeminivirus, Wheat dwarf virus (WDV)

Wheat soil-borne mosaic: genus Furovirus, Wheat soil-borne mosaic virus (SBWMV)
Wheat streak mosaic: genus Rymovirus, Wheat streak mosaic virus
(WSMV) (family Potyviridae)
Wheat yellow leaf: genus Closterovirus, Wheat yellow leaf virus (WYLV)
Wirrega blotch: Drechslera wirreganensis Wallwork et al.
Yellow rust (= stripe rust): Puccinia striiformis Westend
BEAN (PHASEOLUS VULGARIS L.)
Alternaria leaf spot: Alternaria alternata (Fr.:Fr.) Keissl.
Angular leaf spot: Phaeoisariopsis griseola (Sacc.) Ferraris
Anthracnose: Colletotrichum lindemuthianum (Sacc. and Magnus) Lams.Scribner, Teleomorph: Glomerella lindemuthiana Shear
Ashy stem blight (charcoal rot): Macrophomina phaseolina (Tassi)
Goidanich
Bacterial brown spot: Pseudomonas syringae pv. syringae van Hall 1902
Bacterial wilt: Curtobacterium flaccumfaciens pv. flaccumfaciens
(Hedges 1922) Collins and Jones 1983 = Corynebacterium
flaccumfaciens (Hedges 1922) Dowson 1942
Bean common mosaic: genus Potyvirus, Bean common mosaic virus
(BCMV)
Bean curly dwarf mosaic: genus Comovirus, Bean curly dwarf mosaic
virus (BCDMV)
Bean dwarf mosaic: Bean dwarf mosaic virus (BDMV)
Bean golden mosaic: genus Potyvirus, Bean common mosaic virus
(BCMV)
Bean mild mosaic: Bean mild mosaic virus (BMMV)
Bean pod mottle: genus Comovirus, Bean pod mottle virus (BPMV)
Bean rugose mosaic: genus Comovirus, Bean rugose mosaic virus (BRMV)
Bean southern mosaic: genus Sobemovirus, Bean southern mosaic virus
(BSMV)
Bean yellow mosaic: genus Potyvirus, Bean yellow mosaic virus (BYMV)
Bean yellow stipple: Bean yellow stipple virus (BYSV)
Black node disease: Phoma exigua Desmaz. var. diversispora (Bubak)
Boerema = P. diversispora Bubak
Black root rot: Thielaviopsis basicola (Berk. and Broome) Ferraris
Cercospora leaf blotch: Pseudocercospora cruenta (Sacc.) Deighton =
Cercospora cruenta Sacc., Teleomorph: Mycosphaerella cruenta D.H.
Latham, Cercospora canescens Ellis and G. Martin
Clover yellow vein: genus Potexvirus, Clover yellow vein virus (CYVV)
Common blight, fuscous blight: Xanthomonas axonopodis pv. phaseoli
(Smith 1897) Vauterin, Hoste, Kersters, and Swings 1995 =
Xanthomonas campestris pv. phaseoli (Smith 1897) Dye 1978 =
Xanthomonas phaseoli (ex Smith 1997) Gabriel, Kingsley, Hunter, and
Gottwald 1989
Cucumber mosaic: genus Cucumovirus, Cucumber mosaic virus (CMV)
Curly top: genus Hybrigeminivirus, Beet curly top virus (BCTV)
Damping-off and stem rot: Rhizoctonia solani Khn, Teleomorph:
Fusarium root rot: Fusarium solani (Mart.) Sacc. f. sp. phaseoli
(Burkholder) W. C. Snyder and H. N. Hans.
Fusarium yellows: Fusarium oxysporum Schlechtend.:Fr. f. sp. phaseoli
J. B. Kendrick and W. C. Snyder
Gray mold: Botrytis cinerea Pers.:Fr, Teleomorph: Botryotinia fuckeliana
(de Bary) Whetzel
Halo blight: Pseudomonas savastanoi pv. phaseolicola (Burkholder 1926)
Gardan, Bollet, Abu Ghorrah, Grimont, and Grimont 1992 = Pseudomonas syringae pv. phaseolicola (Burkholder 1926) Young, Dye, and
Wilkie 1978
Leaf spot: Phoma exigua Desmaz. var. exigua = Ascochyta phaseolorum
Sacc.
Machismo: Phytoplasma
Peanut mottle: genus Potyvirus, Peanut mottle virus (PMV)
Peanut stunt: genus Cucumovirus, Peanut stunt virus (PSV)
Phyllosticta leaf spot: Phyllosticta phaseolina Sacc.
Phymatotrichum root rot (cotton root rot): Phymatotrichopsis omnivora
(Duggar) Hennebert = Phymatotrichum omnivorum Duggar
Phytopthora blight: Phytophthora nicotianae Breda de Haan var.
parasitica (Dastur) G. M. Waterhouse, Phytophthora phaseoli Thaxt.
Powdery mildew: Erysiphe polygoni DC.
Pythium blight: Pythium debaryanum Auct. non R. Hesse, P. ultimum
Trow
Red node: genus Ilarvirus, Tobacco streak virus (TSV)
Rust: Uromyces appendiculatus (Pers.:Pers.) Unger
Speckle disease: Stagonosporopsis hortensis (Sacc. and Malbr.) Petr. =
Ascochyta boltshauseri Sacc. in Boltshauser
Stipple streak: genus Necrovirus, Tobacco necrosis virus (TNV)
Tobacco ringspot: genus Tobravirus, Tobacco ringspot virus (TRS)
Web blight: Rhizoctonia solani Khn
White mold (Sclerotinia rot): Sclerotinia sclerotiorum (Lib.) de Bary
CITRUS (CITRUS SPECIES)

Algal spot: Cephaleuros virescens Kunze = Cephaleuros mycoides Karst.
(Alga)
Alternaria leaf and fruit spot: Alternaria citri Ell. and Pierce = Alternaria
alternata (Fr.:Fr) Keissl. pv. citri
Black pit: Pseudomonas syringae van Hall 1902
Black root rot: Thielaviopsis basicola (Berk. and Br.) Ferr., Anamorph:
Chalara elegans Nag Raj and Kendrick
Black spot: Guignardia citricarpa Kiely
Blue mold: Penicillium italicum Wehmer
Canker: Xanthomonas axonopodis pv. citri (Hasse 1915) Vauterin, Hoste,
Kersters, and Swings 1995 = Xanthomonas citri (ex Hasse 1915)
Gabriel, Kingsley, Hunter, and Gottwald 1989 = Xanthomonas
campestris pv. citri (Hasse 1915) Dye 1978
Cercospora leaf and fruit spot: Cercospora angolensis Carv. and Mendes
Citrus leaf rugose: genus Ilarvirus, Citrus rugose virus (CRV)
Citrus variegated chlorosis: Xylella fastidiosa Wells, Raju, Weisburg,
Mandelo-Paul, and Brenner 1987
Clitocybe root rot: Armillaria tabescens (Scop.:Fr.) Dennis et al. =
Clitocybe tabescens (Scop.:Fr.) Bres.
Crinkly leaf: genus Ilarvirus, Citrus crinkly leaf virus (CCLV)
Diplodia gummosis: Physalospora rhodina (Berk. and Curt.) Cke.,
Anamorph: Diplodia natalensis Pole-Evans
Dodder: Cuscuta spp.
Dothiorella gummosis: Botryosphaeria dothidea (Moug.:Fr.) Ces. and de
Not., Anamorph: Dothiorella gregaria Sacc.
Exocortis: Citrus exocortis viroid
Fusarium wilt: Fusarium oxysporum Schlecht.
Gray mold: Botrytis cinerea Pers.:Fr., Teleomorph: Botryotinia fuckeliana
(de Bary.) Whetzel
Greasy spot: Mycosphaerella citri Whiteside, Anamorph: Cercospora
citri grisea Fisher
Green mold: Penicillium digitatum Sacc.
Greening: Citrus greening organism (Nonculturable, phloem-restricted
Gram-negative bacteria) Candidatus Liberobacter asiaticum and
Candidatus Liberobacter africanum
Heart rot: Ganoderma sessile Murrill, G. applanatum (Pers.) Pat.
Hendersonula branch wilt: Hendersonula toruloidea Nattrass
Infectious variegation: genus Ilarvirus, Citrus infectious variegation virus
(CIVV)
Lime anthracnose: Gloeosporium limetticola Clausen

Mal secco: Phoma tracheiphila (Petri) Kentschaveli and Gikachvili =
Deuterophoma tracheiphila Petri
Melanose: Diaporthe citri Wolf = Diaporthe medusaea Nits., Anamorph:
Phomopsis citri Fawc.
Phytophthora gummosis, foot rot, root rot, and brown fruit rot: Phytophthora citrophthora (R. E. Sm. and E. H. Sm.) Leonian, P. hibernalis
Carne, P. parasitica Dast., P. syringae (Kleb.) Kleb.
Pink disease: Corticium salmonicolor Berk. and Br.
Postbloom fruit drop disease and anthracnose: Glomerella cingulata
(Ston.) Spauld. and Schrenk, Anamorph: Colletotrichum
gloeosporioides (Penz.) Sacc.
Ringspot: Citrus ringspot virus (CRSV)
Satsuma dwarf: Citrus satsuma dwarf virus (CSDV)
Scab: Elsinoe fawcettii Bitanc. and Jenkins
Sclerotinia twig blight, fruit rot, and root rot: Sclerotinia sclerotiorum
(Lib.) de Bary
Septoria spot: Septoria citri Pass.
Shoestring root rot: Armillaria mellea (Vahl.:Fr.) Kummer = Armillariella
mellea (Vahl.:Fr.) Karst.
Sooty blotch: Gloeodes pomigena (Schw.) Colby
Sooty mold: Capnodium citri Berk. and Desm., Capnodium citricola
McAlp.
Sour rot: Geotrichum candidum Lk. = Oospora lactis (Fres.) Sacc.
Stubborn: Spiroplasma citri Saglio, I.hospital, Lafleche, Dupont, Bove,
Mouches, Rose, Coan, and Clark 1986
Sweet orange scab: Elsinoe australis Bitanc. and Jenkins
Tatter leaf and citrange stunt: genus Capillovirus, Citrus tatter leaf virus
(CTLV)
Tristeza: genus Closterovirus, Citrus tristeza virus (CTV)
Witches-broom: Phytoplasma
CORN (ZEA MAYS L.)
American wheat striate: genus Cytorhabdovirus, American wheat striate
mosaic virus (AWSMV)
Anthracnose leaf blight: Colletotrichum graminicola (Ces.) G.W. Wils.,
Teleomorph: Glomerella graminicola Politis, Glomerella tucumanensis
(Speg.) Arx and E. Mller, Anamorph: Colletotrichum falcatum Went
Bacterial leaf blight and stalk rot: Acidovorax avenae ssp. avenae (Manns
1909) Willems, Goor, Thielemans, Gillis, Kersters, and De Ley 1990 =
Pseudomonas avenae ssp. avenae Manns 1909
Bacterial leaf spot: Xanthomonas vasicola pv. holcicola (Elliott 1930)
Bacterial stalk rot: Enterobacter dissolvens (Rosen 1922) Brenner,
McWhorter, Kai, Steigerwalt, and Farmer 1988 = Erwinia dissolvens
(Rosen 1922) Burkholder 1948
Bacterial stalk and top rot: Erwinia carotovora ssp. carotovora (Jones
1901) Bergey, Harrison, Breed, Hammer, and Huntoon 1923, Erwinia
chrysanthemi pv. zeae (Sabet 1954) Victoria, Arboleda, and Munoz
1975
Bacterial stripe: Burkholderia andropogonis (Smith 1911) Gillis, Van
Van, Bardin, Goor, Hebbar, Willems, Segers, Kersters, Heulin, and
Fernandez 1995 = Pseudomonas andropogonis (Smith 1911) Stapp
1928
(BSMV)
(BYDV)
Brome mosaic: Brome mosaic virus (BMV)
Brown spot: Physoderma maydis (Miyabe) Miyabe
Brown stripe downy mildew: Sclerophthora rayssiae Kenneth et al. var.
zeae Payak and Renfro
Cereal chlorotic mottle: Cereal chlorotic mottle virus (CCMV)
Charcoal rot: Macrophomina phaseolina (Tassi) Goidanich
Chocolate spot: Pseudomonas syringae pv. coronafaciens (Elliott 1920)
Common corn rust: Puccinia sorghi Schwein.
Common smut: Ustilago zeae (Beckm.) Unger = U. maydis (DC.) Corda
Corn chlorotic vein banding: Corn chlorotic vein banding virus (CCVBV)
Corn stunt: Spiroplasma kunkelii Whitcomb, Chen, Williamson, Liao,
Tully, Bove, Mouchers, Rose, Coan, and Clark 1986
Crazy top downy mildew: Sclerophthora macrospora (Sacc.)
Thirumalachar et al. = Sclerospora macrospora Sacc.
Cynodon chlorotic streak: Cynodon chlorotic streak virus (CCSV)
Didymella leaf spot: Didymella exitalis (Morini) E. Muller
Diplodia ear rot: Diplodia maydis (Berk.) Sacc.
Diplodia ear rot and stalk rot: Diplodia frumenti Ellis and Everh.,
Teleomorph: Botryosphaeria festucae (Lib.) Arx and E. Mller
Diplodia leaf spot: Stenocarpella macrospora (Earle) Sutton (Mitosporic

fungi) = Diplodia macrospora Earle
Ergot: Claviceps gigantea Fuentes et al., Anamorph: Sphacelia sp.
False smut: Ustilaginoidea virens (Cooke) Takah.
Fusarium ear and stalk rot: Fusarium subglutinans (Wollenweb. and
Reinking) P. E. Nelson et al. = F. moniliforme J. Sheld. var.
subglutinans Wollenweb. and Reinking
Fusarium stalk rot: Fusarium moniliforme J. Sheld., Teleomorph:
Gibberella fujikuroi (Sawada) Ito in Ito and K. Kimura, Fusarium
avenaceum (Fr.:Fr.) Sacc., Teleomorph: Gibberella avenacea R. J. Cooke
Gibberella ear and stalk rot: Gibberella zeae (Schwein.) Petch,
Anamorph: Fusarium graminearum Schwabe
Gosss bacterial wilt: Clavibacter michiganensis ssp. nebraskensis
(Vidaver and Mandel 1974) Davis, Gillaspie, Vidaver, and Harris 1984
Gray leaf spot: Cercospora sorghi Ellis and Everh. = C. sorghi Ellis and
Everh. var. maydis Ellis and Everh., C. zeae-maydis Tehon and E. Y.
Daniels
Green ear downy mildew: Sclerospora graminicola (Sacc.) J. Schrt.
Head smut: Sphacelotheca reiliana (Kuhn) G. P. Clinton = Sporisorium
holci-sorghi (Rivolta) K. Vanky
Holcus spot: Pseudomonas syringae pv. syringae van Hall 1902
Hyalothyridium leaf spot: Hyalothyridium maydis Latterell and Rossi
Java downy mildew: Peronosclerospora maydis (Racib.) C. G. Shaw =
Sclerospora maydis (Racib.) Butler
Johnsongrass mosaic: genus Potyvirus, Johnsongrass mosaic virus
(JGMV)
Late wilt: Cephalosporium maydis Samra et al.
Maize bushy stunt: Phytoplasma
Maize chlorotic dwarf: genus Waikavirus, Maize chlorotic dwarf virus
(MCDV)
Maize chlorotic mottle: genus Machlomovirus, Maize chlorotic mottle
virus (MCMoV)
Maize dwarf mosaic: genus Potyvirus, Maize dwarf mosaic virus
(MDMV)
Maize leaf fleck: Maize leaf fleck virus (MLFV)
Maize line: Maize line virus (MLV)
Maize mosaic: genus Nucleorhabdovirus, Maize mosaic virus (MMV)
Maize pellucid ringspot: Maize pellucid ringspot virus (MPRV)
Maize rayado fino: Maize rayado fino virus (MRFV)
Maize red stripe: Maize red stripe virus (MRSV)
Maize ring mottle: Maize ring mottle virus (MRMV)
Maize rough dwarf: genus Fijivirus, Maize rough dwarf virus (MRDV)
Maize sterile stunt: Maize sterile stunt virus (MSSV)

Maize streak: genus Monogeminivirus, Maize streak virus (MSV)
Maize stripe: genus Tenuivirus, Maize stripe virus (MStV)
Maize tassel abortion: Maize tassel abortion virus (MTAV)
Maize vein enation: Maize vein enation virus (MVEV)
Maize wallaby ear: Maize wallaby ear virus (MWEV)
Maize white leaf: Maize white leaf virus (MWLV)
Maize white line mosaic: Maize white line mosaic virus (MWLMV)
Millet red leaf: Millet red leaf virus (MRLV)
Northern cereal mosaic: Northern cereal mosaic virus (NCMV)
Northern corn leaf blight: Setosphaeria turcica (Luttrell) K. J. Leonard
and E. G. Suggs, Anamorph: Exserohilum turcicum (Pass.) K. J. Leonard and E. G. Suggs = Helminthosporium turcicum Pass.
Northern corn leaf spot: Cochliobolus carbonum R. R. Nelson,
Anamorph: Bipolaris zeicola (G. L. Stout) Shoemaker =
Helminthosporium carbonum Ullstrup
Oat pseudorosette: Oat pseudorosette virus
Phaeocytostroma stalk rot and root rot: Phaeocytostroma ambiguum
(Mont.) Petr. and Syd. = Phaeocytosporella zeae G. L. Stout
Phaeosphaeria leaf spot: Phaeosphaeria maydis (P. Henn.) Rane, Payak,
and Renfro = Sphaerulina maydis P. Henn.
Philippine downy mildew: Peronosclerospora philippinensis (W. Weston)
C. G. Shaw = Sclerospora philippinensis W. Weston
Pyrenochaeta stalk rot and root rot: Phoma terrestris E. M. Hans. =
Pyrenochaeta terrestris (E. M. Hans.) Gorenz et al.
Pythium root rot: Pythium spp., P. arrhenomanes Drechs., P. graminicola
Subramanian
Pythium stalk rot: Pythium aphanidermatum (Edson) Fitzp. = P. butleri L.
Subramanian
Red kernel disease: Epicoccum nigrum Link
Rhizoctonia root rot and stalk rot: Rhizoctonia solani Khn, R. zeae
Voorhees
virus (RBSDV)
Rostratum leaf spot: Setosphaeria rostrata K. J. Leonard, Anamorph:
Exserohilum rostratum (Drechs.) K. J. Leonard and E. G. Suggs =
Helminthosporium rostratum Drechs.
Selenophoma leaf spot: Selenophoma sp. (Mitosporic fungi)
Sheath rot: Gaeumannomyces graminis (Sacc.) Arx and D. Olivier
Shuck rot: Myrothecium gramineum Lib.
Sorghum downy mildew: Peronosclerospora sorghi (W. Weston and

Uppal) C. G. Shaw = Sclerospora sorghi W. Weston and Uppal
Sorghum mosaic: genus Potyvirus, Sorghum mosaic virus (SrMV)
Southern corn leaf blight: Cochliobolus heterostrophus (Drechs.) Drechs.,
Anamorph: Bipolaris maydis (Nisikado and Miyake) Shoemaker =
Helminthosporium maydis Nisikado and Miyake
Southern corn rust: Puccinia polysora Underw.
Southern leaf spot: Stenocarpella macrospora (Earle) Sutton = Diplodia
macrospora Earle
Spontaneum downy mildew: Peronosclerospora spontanea (W. Weston)
C. G. Shaw = Sclerospora spontanea W. Weston
Stewarts disease (bacterial wilt): Erwinia stewartii (Smith 1898) Dye 1963
Sugarcane downy mildew: Peronosclerospora sacchari (T. Miyake) Shirai
and Hara = Sclerospora sacchari T. Miyake
Sugarcane Fiji disease: genus Fijivirus, Sugarcane Fiji disease virus
(FDV)
Sugarcane mosaic: genus Potyvirus, Sugarcane mosaic virus (SCMV)
Tar spot: Phyllachora maydis Maubl.
Trichoderma ear rot and root rot: Trichoderma viride Pers.:Fr. =
T. lignorum (Tode), Teleomorph: Hypocrea sp.
Tropical corn rust: Physopella pallescens (Arth.) Cummins and
Ramachar, P. zeae (Mains) Cummins and Ramachar = Angiopsora zeae
Mains
White ear rot, root, and stalk rot: Stenocarpella maydis (Berk.) Sutton =
Diplodia zeae (Schwein.) Lv.
Witchweed disease: Striga asiatica (L.) Kuntze = S. lutea Lour.,
S. hermonthica (Del.) Benth. (parasitic plants)
Yellow leaf blight: Phyllosticta maydis D. C. Arny and R. R. Nelson,
Teleomorph: Mycosphaerella zeae-maydis Mukunya and Boothroyd,
Ascochyta ischaemi Sacc.
Zonate leaf spot: Gloeocercospora sorghi Bain and Edgerton ex Deighton
COTTON (GOSSYPIUM SPECIES)
Anthocyanosis: Cotton anthocyanosis virus
Anthracnose: Glomerella gossypii Edgerton, Anamorph: Colletotrichum
gossypii Southworth
Areolate mildew: Mycosphaerella areola J. Ehrlich and F.A. Wolf,
Anamorph: Ramularia gossypii (Speg.) Cif. = Cercosporella gossypii
Speg.
Ascochyta blight: Ascochyta gossypii Woronichin

Bacterial blight: Xanthomonas axonopodis pv. malvacearum (Smith 1901)
Vauterin, Hoste, Kersters, and Swings 1995 = X. campestris pv.
malvacearum (Smith 1901) Dye 1978
Fusarium wilt: Fusarium oxysporum Schlechtend.:Fr. f. sp. vasinfectum
(Atk.) W. C. Snyder and H. N. Hans.
Leaf crumple: genus Bigeminivirus, Cotton leaf crumple virus (CLCrV)
Leaf curl: Cotton leaf curl virus
Leaf mottle: Cotton leaf mottle virus
Leaf spots: Alternaria macrospora A. Zimmerm., A. alternata (Fr.:Fr.)
Keissl., Mycosphaerella gossypina (Atk.) Earle
Phyllody: Phytoplasma
Powdery mildews: Leveillula taurica (Lev) G. Arnaud, Anamorph:
Oidiopsis sicula Scalia, Oidiopsis gossypii (Wakef.) Raychaudhuri,
Salmonia malachrae (Seaver) Blumer and E. Muller
Sclerotium stem and root rot: Sclerotium rolfsii Sacc., Teleomorph:
Athelia rolfsii (Curzi) Tu and Kimbrough
Small leaf: Phytoplasma
Southwestern cotton rust: Puccinia cacabata Arth. and Holw. in Arth.
Tropical cotton rust: Phakopsora gossypii (Lagerh.) Hiratsuka
CRUCIFERS
Crops: cabbage (Brassica oleracea var. capitata L.), cauliflower (B.
oleracea var. botrytis L.), broccoli (B. oleracea L. var. italica), brussels
sprouts (B. oleracea var. gemmifera Zenk.), knol khol (B. oleracea var.
gongylodes L.), kale (B. oleracea var. sabellica L.), collards (B. oleracea
var. viridis L.), turnip [B. rapa var. utilis (D.C.) Metzg.], spinach mustard
(B. rapa L. var. perviridis Bailey), mustard (B. juncea L. var. crispifolia
Bailey), oilseed rape (B. napus L. ssp. oleifera), black mustard [B. nigra (L.)
W. Koch], and radish (Raphanus sativus L.)
Alternaria black spot: Alternaria brassicae (Berk.) Sacc., A. brassicicola
(Schwein.) Wiltshire, A. raphani Groves and Skolko
Anthracnose: Colletotrichum higginsianum Sacc. in Higgins
Bacterial leaf spot: Pseudomonas syringae pv. maculicola (McCulloch

Bacterial soft rot Erwinia: Erwinia carotovora (Jones 1901) Bergey,
Harrison, Breed, Hammer, and Huntoon 1923
Bacterial soft rot Pseudomonas: Pseudomonas marginalis pv. marginalis
(Brown 1918) Stevens 1925
Black leg and Phoma root rot: Leptosphaeria maculans (Desmaz.) Ces.
and De Not., Anamorph: Phoma lingam (Tode:Fr.) Desmaz.
Black root (Aphanomyces): Aphanomyces raphani Kendrick
Black rot: Xanthomonas campestris pv. campestris (Pammel 1985)
Dowson 1939
Cauliflower mosaic: genus Caulimovirus, Cauliflower mosaic virus
(CaMV)
Cercospora leaf spot: Cercospora brassicicola Henn.
Clubroot: Plasmodiophora brassicae Woronin
Conn 1942
Damping-off: Fusarium spp., Pythium spp.
Downy mildew: Peronospora parasitica (Pers.:Fr.) Fr.
Fusarium yellows: Fusarium oxysporum Schlechtend.:Fr.
(de Bary) Whetzel
Light leaf spot: Pyrenopeziza brassicae Sutton and Rawl
Phytophthora root rot: Phytophthora megasperma Drechs.
Powdery mildew: Erysiphe polygoni DC.
Radish mosaic: genus Comovirus, Radish mosaic virus (RaMV)
Ring spot: Mycosphaerella brassicicola (Duby) Lindau in Engl. and
Prantl, Anamorph: Asteromella brassicae (Chev.) Boerema and Van
Kesteren
Root rot: Rhizoctonia solani Khn, Teleomorph: Thanatephorus
cucumeris (A. B. Frank) Donk
Scab: Streptomyces spp.
Sclerotinia stem rot and watery soft rot: Sclerotinia sclerotiorum (Lib.)
de Bary
Turnip mosaic: genus Potyvirus, Turnip mosaic virus (TuMV)
Verticillium wilt: Verticillium albo-atrum Reinke and Berthier, V. dahliae
Kleb.
White leaf spot and gray stem: Pseudocercosporella capsellae (Ellis and
Everh.) Deighton
White rust: Albugo candida (Pers.) Kunze
Xanthomonas leaf spot: Xanthomonas campestris pv. armoraciae
(McCulloch 1929) Dye 1978
Yellows: genus Luteovirus, Beet western yellows virus (BWYV)
CUCURBITS
Crops: melon, muskmelon, and cantaloupe (Cucumis melo L.), cucumber (Cucumis sativus L.), squash, gourd, and pumpkin (Cucurbita pepo L.),
watermelon [Citrullus lanatus (Thunb.) Matsum and Nakai], ash gourd and
wax gourd [Benincasa hispida (Thumb.) Cogn.], bottle gourd [Lagenaria
siceraria (Mol.) Standl.], snake gourd (Trichosanthes anguina L.), and
ribbed gourd [Luffa acutangula (L.) Roxb.]
Alternaria leaf blight: Alternaria cucumerina (Ellis and Everh.) J. A.
Elliott
Alternaria leaf spot: Alternaria alternata (Fr.:Fr.) Keissl. f. sp. cucurbitae
Angular leaf spot: Pseudomonas syringae pv. lachrymans (Smith and
Bryan 1915) Young, Dye, and Wilkie 1978
Anthracnose: Colletotrichum orbiculare (Berk. and Mont.) Arx =
C. lagenarium (Pass.) Ellis and Halst, Teleomorph: Glomerella
lagenarium Stevens
Aster yellows: Phytoplasma
Bacterial fruit blotch: Acidovorax avenae ssp. citrulli (Schaad, Sowell,
Goth, Colwell, and Webb 1978) Willems, Goor, Thielemans, Gillis,
Kersters, and De Ley 1992 = Pseudomonas pseudoalcaligenes ssp.
citrulli Schaad, Sowell, Goth, Colwell, and Webb 1978
Bacterial leaf spot: Xanthomonas cucurbitae (ex Bryan 1926) Vauterin,
Hoste, Kersters, and Swings 1995 = Xanthomonas campestris pv.
cucurbitae (Bryan 1926) Dye 1978
Bacterial soft rot: Erwinia carotovora ssp. carotovora (Jones 1901)
Bergey, Harrison, Breed, Hammer, and Huntoon 1923
Bacterial wilt: Erwinia tracheiphila (Smith 1895) Bergey, Harrison,
Breed, Hammer, and Huntoon 1923
Belly rot: Rhizoctonia solani Khn, Teleomorph: Thanatephorus
cucumeris (A. B. Frank) Donk
Brown spot: Pantoea ananas pv. ananas (Serrano 1928) Mergaert,
Verdonck, and Kersters = Erwinia ananas Serrano 1928
Cephalosporium root rot: Acremonium spp. = Cephalosporium spp.

Cercospora leaf spot: Cercospora citrullina Cooke
Chlorotic leaf spot: genus Potyvirus, Bean yellow mosaic virus (BYMV)
Choanephora fruit rot: Choanephora cucurbitarum (Berk. and Ravenel)
Thaxt.
Collapse of melon: Monosporascus eutypoides (Petrak) Arx =
Bitrimonospora indica Sivanesans et al.
Corynespora blight/target spot: Corynespora cassiicola (Berk. and M. A.
Curtis) C. T. Wei
Crater rot: Myrothecium roridum Tode:Fr.
Crown and foot rot: Fusarium solani (Mart.) Sacc. f. sp. cucurbitae W. C.
Snyder and H. N. Hans., Teleomorph: Nectria haematococca Berk. and
Broome
Cucumber green mottle: genus Tobamovirus, Cucumber green mottle
mosaic virus (CGMMV)
Cucumber vein yellowing: Cucumber vein yellowing virus (CVYV)
Curly top: genus Hybrigeminivirus, Beet curly top virus (BCTV)
Downy mildew: Pseudoperonospora cubensis (Berk. and M. A. Curtis)
Rostovzev
Fusarium wilt: F. oxysporum f. sp. melonis W. C. Snyder and H. N. Hans.
(muskmelon), Fusarium oxysporum f. sp. cucumerinum J. H. Owen
(cucumber), F. oxysporum f. sp. niveum (E. F. Sm.) W. C. Snyder and
H. N. Hans. (watermelon), F. oxysporum f. sp. momordicae Sun and
Huang (bitter melon), F. oxysporum Schlechtend.:Fr. f. sp. benincasae
Gerlagh and Ester (wax gourd), F. oxysporum f. sp. lagenariae Matuo
and Yamamoto (calabash gourd), F. oxysporum f. sp. luffae Lawai et al.
(vegetable sponge)
(de Bary) Whetzel
Gummy stem blight: Didymella bryoniae (Auersw.) Rehm =
Mycosphaerella melonis (Pass.) Chiu and J. C. Walker, Anamorph:
Phoma cucurbitacearum (Fr.:Fr.) Sacc.
Lasiodiplodia fruit rot: Lasiodiplodia theobromae (Pat.) Griffon and
Maubl. = Diplodia natalensis Pole-Evans
Lettuce infectious yellows: genus Closterovirus, Lettuce infectious
yellows virus (LIYVV)
Melon leaf curl: Melon leaf curl virus (MLCV)
Melon necrotic spot: genus Carmovirus, Melon necrotic spot virus
(MNSV)
Monosporascus root rot: Monosporascus cannonballus Pollack and

Uecker
Mosaic: genus Potyvirus, Papaya ringspot virus W strain (PRSV-W)
Muskmelon vein necrosis: genus Carlavirus, Muskmelon vein necrosis
virus (MkVNV)
Myrothecium canker: Myrothecium roridum Tode:Fr.
Net spot: Leandria momordicae Rangel
Phoma blight: Phoma exigua Desmaz. var. exigua = Ascochyta
phaseolorum Sacc.
Phomopsis black stem: Phomopsis sclerotiodes Van Kesteren
Phyllosticta leaf spot: Phyllosticta cucurbitacearum Sacc.
Phytophthora root rot: P. capsici Leonian
Powdery mildew: Podosphaera (sect. Sphaerotheca) xanthii (Castagne)
U. Braun and N. Shishkoff = Sphaerotheca fuliginea (Schlechtend.:Fr.)
Pollacci = Sphaerotheca fusca (Fr.) S. Blumer, Erysiphe
cichoracearum DC.
Purple stem: Diaporthe melonis Beraha and OBrien, Anamorph:
Phomopsis cucurbitae McKeen
Scab: Cladosporium cucumerinum Ellis and Arth.
Sclerotinia stem rot: Sclerotinia sclerotiorum (Lib.) de Bary
Septoria leaf blight: Septoria cucurbitacearum Sacc.
Squash leaf curl: genus Bigeminivirus, Squash leaf curl virus (SqLCV)
Squash mosaic: genus Comovirus, Squash mosaic virus (SqMV)
Sudden wilt: Pythium aphanidermatum (Edson) Fitzp.
Tobacco ringspot: genus Tobravirus, Tobacco ringspot virus (TobRSV)
Tomato ringspot: genus Nepovirus, Tomato ringspot virus (TRSV)
(TSWV)
Ulocladium leaf spot: Ulocladium consortiale (Thuem.) E. Simmons
Kleb.
Watermelon mosaic: genus Potyvirus, Watermelon mosaic virus (WMV)
Web blight: Rhizoctonia solani Khn
Zucchini yellows: genus Potyvirus, Zucchini yellows mosaic virus
(ZYMV)
GRAPE (VITIS SPECIES)
Alfalfa mosaic: genus Alfamovirus, Alfalfa mosaic virus (AMV)
Alternaria rot: Alternaria alternata (Fr.:Fr.) Keissl.
Angular leaf scorch: Pseudopezicula tetraspora Korf, R. C. Pearson, and

Zhuang, Anamorph: Phialophora sp.
Angular leaf spot: Mycosphaerella angulata W. A. Jenkins, Anamorph:
Cercospora brachypus Ellis and Everh.
Anthracnose: Elsinoe ampelina Shear, Anamorph: Sphaceloma
ampelinum de Bary
Arabis mosaic: genus Nepovirus, Arabis mosaic virus (ArMV)
Armillaria root rot: Armillaria mellea (Vahl:Fr.) P. Kumm., Anamorph:
Rhizomorpha subcorticalis Pers.
Artichoke Italian latent: genus Nepovirus, Artichoke Italian latent virus
(AILV)
Bacterial blight: Xylophilus ampelinus (Panagopoulos 1969) Willems,
Gillis, Kersters, Van den Broecke, and De Ley 1987 = Xanthomonas
ampelina Panagopoulos 1969
Bitter rot: Greeneria uvicola (Berk. and M. A. Curtis) Punithalingam =
Melanconium fuligineum Lams.-Scrib. and Viala
Black dead arm: Botryosphaeria stevensii Shoemaker, Anamorph:
Diplodia mutila (Fr.:Fr.) Mont.
Black rot: Guignardia bidwellii (Ellis) Viala and Ravaz, Anamorph:
Phyllosticta ampelicida (Engelm.) van der Aa, G. bidwellii (Ellis)
Viala and Ravaz f. muscadinii Luttrell
Bois noir (black wood disease): Phytoplasma
Bratislava mosaic: Bratislava mosaic virus (BMV)
Broad bean wilt: genus Fabavirus, Broad bean wilt virus (BBWV)
Cercospora leaf spot: Phaeoramularia dissiliens (Duby) Deighton =
Cercospora sp.
Cladosporium leaf spot: Cladosporium viticola Cesati
Cladosporium rot: Cladosporium herbarum (Pers.:Fr.) Link, Teleomorph:
Mycosphaerella tassiana (De Not.) Johans
Conn 1942
Dematophora root rot: Rosellinia necatrix Prill., Anamorph:
Dematophora necatrix R. Hartig
Diplodia cane dieback: Lasiodiplodia theobromae (Pat.) Griffon and
Maubl. = Diplodia natalensis Pole-Evans
Downy mildew: Plasmopara viticola (Berk. and M. A. Curtis) Berl and
De Toni in Sacc.
Esca (black measles, apoplexy), grapevine decline: Esca is a disease complex or a complex of diseases caused by several pathogens. Phellinus
igniarius (L.:Fr.) Qul., Stereum hirsutum (Willd.:Fr.) S. F. Gray,
Phaeomoniella chlamydospora Crous et W. Gams = Phaeoacremonium
chlamydosporum, Phaeoacremonium aleophilum, Phaeoacremonium
inflatipes, and Fomitiporia punctata, Trametes hirsute = Coriolus

hirsutus, Trametes versicolor (= Coriolus versicolor), Phaeomoniella
chlamydospora, and several species of Phaeoacremonium
Eutypa dieback: Eutypa lata (Pers.:Fr.) Tul. and C. Tul. = E. armeniacae
Hansf. and M. V. Carter, Anamorph: Libertella blepharis A. L. Smith =
Cytosporina sp.
Fanleaf: genus Nepovirus, Grapevine fanleaf virus (GFLV)
Flavescence dore: Phytoplasma
Grapevine Bulgarian latent: genus Nepovirus, Grapevine Bulgarian latent
virus (GBLV)
Grapevine chrome mosaic: genus Nepovirus, Grapevine chrome mosaic
virus (GCMV)
Grapevine yellows: Phytoplasma
(de Bary) Whetzel
Isariopsis leaf spot: Mycosphaerella personata Higgins, Anamorph:
Pseudocercospora vitis (Lv.) Speg. = Isariopsis clavispora (Berk. and
Cooke) Sacc.
Leaf blotch: Briosia ampelophaga Cavara
Leaf roll: genus Closterovirus, Grapevine leaf roll-associated virus
(GLRaV)
Leaf spots: Asperisporium minutulum (Sacc.) Deighton, Phaeoramularia
heterospora (Ellis and B. T. Galloway) Deighton
Mlanose: Septoria ampelina Berk. and M. A. Curtis
Peach rosette mosaic: genus Nepovirus, Peach rosette mosaic virus
(PRMV)
Petunia asteroid mosaic: genus Tombusvirus, Petunia asteroid mosaic
virus (PAMV)
Phomopsis leaf spot (Excoriose): Phomopsis viticola (Sacc.) Sacc.
Phytophthora crown and root rot: P. cinnamomi Rands, P. megasperma
Drechs.
Pierces disease: Xylella fastidiosa Wells, Raju, Hung, Weisburg,
Mandelco-Paul, and Brenner
Powdery mildew: Uncinula necator (Schwein.) Burrill, Anamorph:
Oidium tuckeri Berk.
Raspberry ringspot: genus Nepovirus, Raspberry ringspot virus (RRV)
Ripe rot: Glomerella cingulata (Stoneman) Spauld. and H. Schrenk,
Penz.
Roesleria root rot: Roesleria subterranea (Weinmann) Redhead =

R. hypogaea Thuem. and Pass.
Rotbrenner: Pseudopezicula tracheiphila (Mll.-Thurg.) Korf and Zhuang
= Pseudopeziza tracheiphila Mll.-Thurg., Anamorph: Phialophora
tracheiphila (Sacc. and Sacc.) Korf
Rust: Physopella ampelopsidis (Dietel and P. Syd.) Cummins and
Ramachar
Sowbane mosaic: genus Sobemovirus, Sowbane mosaic virus (SoMV)
Strawberry latent ringspot: genus Nepovirus, Strawberry latent ringspot
virus (SLRV)
Tar spot: Rhytisma vitis Schwein.
Tobacco necrosis: genus Necrovirus, Tobacco necrosis virus (TNV)
Tobacco ringspot virus decline: genus Tobravirus, Tobacco ringspot virus
(TRSV)
Tomato black ring: genus Nepovirus, Tomato black ring virus (TBRV)
Tomato ringspot virus decline: genus Nepovirus, Tomato ringspot virus
(ToRSV)
White rot: Coniella diplodiella (Speg.) Petr. and Syd.
Yellow speckle: Viroid
Zonate leaf spot: Cristulariella moricola (Hino) Redhead =
C. pyramidalis A. M.Waterman and R. P. Marshall, Teleomorph:
Grovesinia pyramidalis M. Cline et al.
OATS (AVENA SATIVA L.)

Bacterial blight (halo blight): Pseudomonas syringae pv. coronafaciens
(Elliott 1920) Young, Dye, and Wilkie 1978
Bacterial stripe blight: Pseudomonas syringae pv. striafaciens (Elliott
Covered smut: Ustilago segetum (Bull.:Pers.) Roussel = U. kolleri Wille
Crown rust: Puccinia coronata Corda
Downy mildew: Sclerophthora macrospora (Sacc.) Thirum. et al. =

Sclerospora macrospora Sacc.
Ergot: Claviceps purpurea (Fr.:Fr.) Tul., Anamorph: Sphacelia segetum Lev.
Fusarium foot rot: Fusarium culmorum (Wm. G. Sm.) Sacc.
Gray snow mold (= Typhula blight): Typhula incarnata Fr.,
T. ishikariensis Imai var. idahoensis (Remsb.) Arsvoll and Smith,
T. ishikariensis Imai var. ishikariensis, T. ishikariensis Imai var.
canadensis Smith and Arsvoll
Head blight: Fusarium graminearum Schwabe, Teleomorph: Gibberella
zeae (Schwein.) Petch, Bipolaris sorokiniana (Sacc.) Shoemaker,
Teleomorph: Cochliobolus sativus (Ito and Kuribayashi) Drechs. ex
Dastur
Leaf blotch: Drechslera avenacea (M. A. Curtis ex Cooke) Shoemaker =
Helminthosporium avenaceum M. A. Curtis ex Cooke, D. avenae
(Eidam) Scharif = H. avenae Eidam, Teleomorph: Pyrenophora avenae
Ito and Kuribayashi
Loose smut: Ustilago avenae (Pers.) Rostr.
Oat blue dwarf: genus Marafivirus, Oat blue dwarf virus (OBDV)
Oat mosaic: genus Bymovirus, Oat mosaic virus (OMV)
Oat necrotic mottle: Oat necrotic mottle virus (ONMV)
Oat pseudorosette: genus Tenuivirus, Oat pseudorosette virus (OPRV)
Oat red leaf: genus Luteovirus, Barley yellow dwarf virus (BYDV)
Fusarium nivale Ces. ex Sacc., Teleomorph: Monographella nivalis
Powdery mildew: Blumeria graminis (DC.) E. O. Speer f. sp. = Erysiphe
graminis DC. f. sp. avenae Em. Marchal, E. graminis DC., Anamorph:
Oidium monilioides (Nees) Link
Sharp eyespot: Rhizoctonia solani Kuhn, Teleomorph: Thanatephorus
cucumeris (A. B. Frank) Donk, Rhizoctonia cerealis Van der Hoeven,
Teleomorph: Ceratobasidium cereale D. Murray and L. L. Burpee
Speckled blotch (Septoria blight): Stagonospora avenae (A. B. Frank)
Bissett = Septoria avenae A. B. Frank, Teleomorph: Phaeosphaeria
avenaria (G. F. Weber) O. Eriksson f. sp. avenaria
Stem rust: Puccinia graminis Pers.
Take-all: Gaeumannomyces graminis (Sacc.) von Arx and Olivier var.
avenae (E. M. Turner) Dennis
Victoria blight: Bipolaris victoriae (F. Meehan and Murphy) Shoemaker,
Teleomorph: Cochliobolus victoriae R. R. Nelson
PEA (PISUM SATIVUM L.)

Alternaria blight: Alternaria alternata (Fr.:Fr.) Keissl.
Anthracnose: Colletotrichum gloeosporioides (Penz.) Penz. and Sacc. in
Penz. = Colletotrichum pisi Pat.
Aphanomyces root rot: Aphanomyces euteiches Drechs. f. sp. pisi
Ascochyta blight: Didymella pinodes (Berk. and A. Bloxam) Petr.,
Anamorph: Ascochyta pinodes L. K. Jones
Ascochyta leaf and pod spot: Ascochyta pisi Lib.
Bacterial blight: Pseudomonas syringae pv. pisi (Sackett 1916) Young,
Black leaf: Fusicladium pisicola Linford
Brown spot: Pseudomonas syringae pv. syringae van Hall 1902
Cercospora leaf spot: Cercospora pisa-sativae Stevenson
Cladosporium blight: Cladosporium cladosporioides (Fresen.) de Vries
f. sp. pisicola = Cladosporium pisicola
Downy mildew: Peronospora viciae (Berk.) de Bary
Gray mold: Botrytis cinerea Pers.:Fr.
Pea enation mosaic: genus Enamovirus, Pea enation mosaic virus (PEMV)
Pea root rot: Fusarium solani (Mart.) Sacc. f. sp. pisi (F. R. Jones) Snyd.
and Hans.
Pea seedborne mosaic: genus Potyvirus, Pea seedborne mosaic virus
(PSbMV)
Pea streak: genus Alfamovirus, Alfalfa mosaic virus (AMV); genus
Carlavirus, Pea streak virus (PeSV)
Pea stunt: genus Carlavirus, Red clover vein mosaic virus (RCVMV)
Powdery mildew: Erysiphe pisi DC., Anamorph: Oidium sp.
Rhizoctonia seedling blight: Thanatephorus cucumeris (A. B. Frank)
Donk, Anamorph: Rhizoctonia solani Kuhn
Sclerotinia rot: Sclerotinia sclerotiorum (Lib.) de Bary
Septoria blotch: Septoria pisi Westend.
Thielaviopsis root rot: Thielaviopsis basicola (Berk. and Broome)
Ferrasis
Wilt: Fusarium oxysporum Schlechtend. f. sp. pisi
PEACH [PRUNUS PERSICA (L.) BATSCH]
Almond enation: genus Nepovirus, Tomato black ring virus (TBRV)
Alternaria rot: Alternaria alternata (Fr.:Fr.) Keissl.
Anthracnose: Colletotrichum gloeosporioides (Penz.) Penz. and Sacc. in
Penz., Teleomorph: Glomerella cingulata (Stoneman) Spauld. and
H. Schrenk
Apricot chlorotic leaf roll: Phytoplasma

Armillaria crown and root rot: Armillaria mellea (Vahl:Fr.) P. Kumm.,
Anamorph: Rhizomorpha subcorticalis Pers.
Bacterial canker: Pseudomonas syringae pv. syringae van Hall 1902
Bacterial spot: Xanthomonas arboricola pv. pruni (Smith 1903) Vauterin,
Hoste, Kersters, and Swings 1995
Botrytis ripe fruit rot: Botrytis cinerea Pers.:Fr., Teleomorph: Botryotinia
Brown rot: Monilinia fructicola (G. Wint.) Honey, Monilinia laxa
(Aderhold and Ruhland) Honey
Constriction canker: Phomopsis amygdali (Delacr.) Tuset and Portilla =
Fusicoccum amygdali Delacr.
Conn. 1942
Cytospora canker: Cytospora leucostoma Sacc., Teleomorph: Leucostoma
persoonii Hhn.
Dark green sunken mottle: genus Closterovirus, Apple chlorotic leaf spot
virus (ACLSV)
Enation: genus Enamovirus, Peach enation virus (PEV)
Gilbertella ripe fruit rot: Gilbertella persicaria (E. D. Eddy) Hesseltine
Gummosis: Botryosphaeria dothidea (Moug.:Fr.) Ces and De Not. =
Botryosphaeria berengeriana De Not., Anamorph: Fusicoccum aesculi
Corda
Leaf curl: Taphrina deformans (Berk.) Tul.
Line pattern: genus Ilarvirus, Prunus necrotic ringspot virus (PNRSV) +
genus Ilarvirus, Apple mosaic virus (ApMV)
Little peach (= yellows): Phytoplasma
Necrotic ring spot: genus Ilarvirus, Prunus necrotic ringspot virus
(PNRSV)
Peach latent mosaic: Peach latent mosaic viroid
Phony peach: Xylella fastidiosa Wells, Raju, Hung, Weisburg, MandelcoPaul, and Brenner 1987
Phymatotrichopsis root rot: Phymatotrichopsis omnivorum (Duggar)
Phytophthora crown and root rot: Phytophthora cactorum (Lebert and
Cohn) J. Schrt., P. cambivora (Petri) Buisman, P. citricola Sawada,
P. citrophthora (R. E. Sm. and E. H. Sm.) Leonian, P. cryptogea
Pethybr. and Lafferty, P. drechsleri Tucker, P. megasperma Drechs., P.
syringae (Kleb.) Kleb.

Powdery mildew: Podosphaera clandestina (Wallr.:Fr.) Lv., Anamorph:
Oidium sp., Podosphaera leucotricha (Ellis. and Everh.) E. S. Salmon.,
Anamorph: O. leucoconium Desmaz.
Prunus stem pitting: genus Nepovirus, Tomato ringspot virus (TmRSV)
Red Suture (yellows): Phytoplasma
Rhizoctonia root rot: Rhizoctonia solani Khn, Teleomorph:
Rosette: Phytoplasma
Rosette and decline: PNRSV + genus Ilarvirus, Prune dwarf virus (PDV)
Rosette mosaic: genus Nepovirus, Peach rosette mosaic virus (PRMV)
Rust: Tranzschelia discolor (Fuckel) Tranzschel and Litv. f. sp. persica
Bolkan, Ogawa, Michailides, and Kable
Scab: Cladosporium carpophilum Thuem. = Fusicladium carpophilum
(Thuem) Oudem, Teleomorph: Venturia carpophila E. E. Fisher
Shot hole: Wilsonomyces carpophilus (Lv) Adaskaveg, Ogawa, and E. E.
Butler = Stigmina carpophila (Lv.) Ellis
Verticillium wilt: Verticillium dahliae Kleb. (Mitosporic fungi)
Willow leaf rosette: genus Nepovirus, Strawberry latent ringspot virus
(SLRV)
X-Disease: Phytoplasma
Yellow bud mosaic: genus Nepovirus, Tomato ringspot virus (TmRSV)
Yellow leaf roll: Phytoplasma
Yellows: Phytoplasma
PEANUT (ARACHIS HYPOGAEA L.)

Alternaria leaf blight: Alternaria tenuissima (Kunze:Fr.) Wiltshire
Alternaria leaf spot: Alternaria arachidis Kulk.
Alternaria veinal necrosis: Alternaria alternata (Fr.:Fr.) Keissl.
Anthracnose: Colletotrichum arachidis Sawada, C. dematium (Pers.:Fr.)
Grove, C. mangenoti Chevaugeon
Aspergillus crown rot: Aspergillus niger Tiegh.
Bacterial wilt: Ralstonia solanacearum (Smith 1896) Yabauuchi, Kosako,
Yano, Hotta, and Nishiuchi 1995
Blackhull: Thielaviopsis basicola (Berk. and Broome) Ferraris,
Synanamorph: Chalara elegans Nag Raj and Kendrick
Botrytis blight: Botrytis cinerea Pers.:Fr., Teleomorph: Botryotinia
Charcoal rot: Macrophomina phaseolina (Tassi) Goidanich = Rhizoctonia

bataticola (Tassi) E. J. Butler
Choanephora leaf spot: Choanephora spp.
Collar rot: Lasiodiplodia theobromae (Pat.) Griffon and Maubl. =
Diplodia gossypina Cooke
Colletotrichum leaf spot: Colletotrichum gloeosporioides (Penz.) Penz.
and Sacc. in Penz., Teleomorph: Glomerella cingulata (Stoneman)
Spauld. and H. Schrenk
Cowpea mild mottle: genus Carlavirus, Cowpea mild mottle virus
(CPMMV)
Cylindrocladium black rot: Cylindrocladium crotalariae (C. A. Loos)
D. K. Bell and Sobers, Teleomorph: Calonectria crotalariae (C. A.
Loos) D. K. Bell and Sobers
Cylindrocladium leaf spot: Cylindrocladium scoparium Morg.,
Teleomorph: Calonectria keyotensis Terishita
Dodder: Cuscuta campestris Yunck. (parasitic plant)
Drechslera leaf spot: Bipolaris spicifera (Bainier) Subramanian =
Drechslera spicifera (Bainier) Arx, Teleomorph: Cochliobolus spicifer
R. R. Nelson
Early leaf spot: Cercospora arachidicola S. Hori, Teleomorph:
Mycosphaerella arachidis Deighton
Fusarium wilt: Fusarium oxysporum Schlechtend.:Fr.
Groundnut crinkle: Groundnut crinkle virus (GCV)
Groundnut eyespot: Groundnut eyespot virus (GESV)
Groundnut rosette: genus Umbravirus, Groundnut chlorotic rosette virus
(GCRV); genus Umbravirus, Groundnut green rosette virus (GGRV)
Groundnut streak: Groundnut streak virus (GSV)
Late leaf spot: Phaeoisariopsis personata (Berk. and M. A. Curtis) Arx =
Cercosporidium personatum (Berk. and M. A. Curtis) Deighton,
Teleomorph: Mycosphaerella berkeleyi Jenk.
Melanosis: Stemphylium botryosum Wallr, Teleomorph: Pleospora tarda
E. Simmons
Myrothecium leaf blight: Myrothecium roridum Tode:Fr.
Olpidium root rot: Olpidium brassicae (Woronin) P. A. Dang.
Peanut bud necrosis: genus Tospovirus, Peanut bud necrosis virus
(PBNV) (family Bunyaviridae)
Peanut chlorotic blotch: genus Potyvirus, Peanut chlorotic blotch virus
(PCBV) (family Potyviridae)
Peanut chlorotic fan-spot: Peanut chlorotic fan-spot virus (PCFSV)
Peanut chlorotic streak: genus Caulimovirus, Peanut chlorotic streak virus
(PCSV)
Peanut clump: genus Furovirus, Peanut clump virus (PCV)
Peanut green mosaic: Peanut green mosaic virus (PGMV)

Peanut mottle: genus Potyvirus, Peanut mottle virus (PeMoV) (family
Potyviridae)
Peanut stripe: genus Potyvirus, Peanut stripe virus (PStV) (family
Potyviridae)
Peanut stunt: genus Cucumovirus, Peanut stunt virus (PSV)
Peanut yellow mottle: Peanut yellow mottle virus (PYMoV)
Peanut yellow spot: genus Tospovirus, Peanut yellow spot virus (PYSV)
Pepper spot and scorch: Leptosphaerulina crassiasca (Sechet) C. R.
Jackson and D. K. Bell
Pestalotiopsis leaf spot: Pestalotiopsis arachidis Satya
Phanerogamic root parasite: Alectna sp. (parasitic plant)
Phoma leaf blight: Phoma microspora Benk. and M. A. Curtis
Phomopsis foliar blight: Phomopsis phaseoli (Desmaz.) Sacc. = P. sojae
Lehman, Teleomorph: Diaporthe phaseolorum (Cooke and Ellis) Sacc.
Phyllosticta leaf spot: Phyllosticta arachidis-hypogaea V. G. Rao,
P. sojicola C. Massal., Teleomorph: Pleosphaerulina sojicola Miura
Powdery mildew: Oidium arachidis Chorin
Pythium peg and root rot: Pythium myriotylum Drechs., P. aphanidermatum (Edson) Fitzp., P. debaryanum Auct. non R. Hesse,
P. irregulare Buisman, P. ultimum Trow
Rhizoctonia root rot: Rhizoctonia solani Khn
Rust: Puccinia arachidis Speg.
Scab: Sphaceloma arachidis Bitancourt and Jenk.
Sclerotinia blight: Sclerotinia minor Jagger, S. sclerotiorum (Lib.) de Bary
Stem rot: Sclerotium rolfsii Sacc, Teleomorph: Athelia rolfsii (Curzi) Tu
and Kimbrough
Kleb.
Web blotch (net blotch): Phoma arachidicola Marasas et al. = Ascochyta
adzamethica Schoschiaschvili, Teleomorph: Didymosphaeria
arachidicola (Chochrjakov) Alcorn = Mycosphaerella arachidicola
Chochrjakov
Zonate leaf spot: Cristulariella moricola (Hino) Redhead, Synanamorph:
Sclerotium cinnomomi Sawada, Teleomorph: Grovesinia pyramidalis
M. Cline et al.
PEAR (PYRUS COMMUNIS L.)

American hawthorne rust: Gymnosporangium globosum (Farl.) Farl.
Anthracnose canker: Pezicula malicorticus (H. Jacks) Nannf., Anamorph:
Cryptosporiopsis curvispora (Peck) Gremmen in Boerema and
Gremmen
Armillaria root rot: Armillaria mellea (Vahl:Fr.) P. Kumm., Anamorph:
Rhizomorpha subcorticalis Pers.
Bitter rot: Glomerella cingulata (Stoneman) Spauld. and Schrenk,
Penz.
Black rot and canker: Botryosphaeria obtusa (Schwein.) Shoemaker,
Anamorph: Sphaeropsis malorum Berk.
Black spot: Alternaria alternata (Fr.:Fr.) Keissl.
Blister canker: Helminthosporium papulosum Berg.
Blister disease: Coniothecium chromatosporum Corda
Botrytis blossom blight: Botrytis cinerea Pers.:Fr., Teleomorph:
Botryotinia fuckeliana (de Bary) Whetzel
Brown rot: Monilinia fructicola (Wint.) Honey, M. laxa (Aderh. and
Ruhl.) Honey
Brown spot: Stemphylium vesicarium (Wallr.) E. Simmons, Teleomorph:
Pleospora allii (Rabenh.) Ces. and De Not.
Cladosporium fruit rot: Cladosporium herbarum (Pers.:Fr.) Link,
Teleomorph: Mycosphaerella tassiana (De Not.) Johans.
Conn 1942
Diplodia canker: Botryosphaeria stevensii Shoemaker = Physalospora
malorum Shear et al., Anamorph: Diplodia mutila (Fr.:Fr.) Mont.
Elsinoe leaf and fruit spot: Elsinoe piri (Woronichin) Jenk., Anamorph:
Sphaceloma pirinum Jenk.
European canker: Nectria galligena Bres. in Strass., Anamorph:
Cylindrocarpon heteronemum (Berk. and Broome) Wollenweb.
European pear rust: Gymnosporangium fuscum R. Hedw. in DC.
Fabraea leaf and fruit spot: Diplocarpon mespili (Sorauer) Sutton =
Fabraea maculata Atk., Anamorph: Entomosporium mespili (DC.)
Sacc.
Fire blight: Erwinia amylovora (Burrill 1882) Winslow, Broadhurst,
Buchanan, Krumwiede, Rogers, and Smith 1920
Fly speck: Schizothyrium pomi (Mont.:Fr.) Arx, Anamorph: Zygophiala

jamaicensis E. Mason
Gibberella canker: Gibberella baccata (Wallr.) Sacc., Anamorph:
Fusarium lateritium Nees:Fr.
Kerns pear rust: Gymnosporangium kernianum Bethel
Late leaf spot: Cercospora minima Tracy and Earle
Mycosphaerella leaf spot: Mycosphaerella pyri (Auersw.) Boerema =
M. sentina (Fr.:Fr.) Schrot., Anamorph: Septoria pyricola (Desmaz.)
Desmaz.
Nectria twig blight: Nectria cinnabarina (Tode) Fr., Anamorph:
Tubercularia vulgaris Tode
Pacific Coast pear rust: Gymnosporangium libocedri (C. Henn.) F. Kern
Pear decline: Phytoplasma
Pear scab: Venturia pirina Aderh., Anamorph: Fusicladium pyrorum
(Lib.) Fuckel
Perennial canker: Neofabrae perennans Kienholz, Anamorph:
Cryptosporiopsis perennans (Zeller and Childs) Wollenweb.
Phyllosticta leaf spot: Phyllosticta sp.
Phytophthora crown and root rot: Phytophthora cactorum (Lebert and
Cohn) J. Schrot.
Powdery mildew: Podosphaera leucotricha (Ellis and Everh.) E. S. Salmon
Pseudomonas canker: Pseudomonas syringae pv. syringae van Hall 1902
Rocky Mountain pear rust: Gymnosporangium nelsonii Arth.
Rosellinia root rot: Rosellinia necatrix Prill., Anamorph: Dematophora
necatrix R. Hartig
Side rot: Phialophora malorum (M. N. Kidd and A. Beaumont)
McColloch
Silver leaf: Chondrostereum pupureum (Pers.:Fr.) Pouzar
Sooty blotch: Gloeodes pomigena (Schwein.) Colby
Thread blight: Corticium stevensii Burt = Pellicularia koleroga Cooke =
Hypochnus ochroleucus Noack
Valsa canker: Valsa ceratosperma (Tode:Fr.) Maire, Anamorph:
Cytospora sacculus (Schwein.) Gvritischvili
Xylaria root rot: Xylaria spp.
PLUM (PRUNUS DOMESTICA L.)
Armillaria crown and root rot: Armillaria mellea (Vahl:Fr.) P. Kumm.,
Anamorph: Rhizomorpha subcorticalis Pers.
Bacterial canker: Pseudomonas syringae pv. syringae van Hall 1902

Bacterial spot: Xanthomonas arboricola pv. pruni (Smith 1903) Vauterin,
Hoste, Kersters, and Swings 1995
Black knot: Apiosporina morbosa (Schwein.:Fr.) Arx, Anamorph:
Fusicladium sp.
Brown rot: Monilinia fructicola (G. Wint.) Honey, Monilinia laxa
(Aderhold and Ruhland) Honey
Line pattern: genus Ilarvirus, Prunus necrotic ringspot virus (PNRSV) +
genus Ilarvirus, Apple mosaic virus (ApMV)
Phony disease: Xylella fastidiosa Wells, Raju, Hung, Weisburg,
Mandelco-Paul, and Brenner 1987
Red Suture (= yellows): Phytoplasma
Rosette mosaic: genus Nepovirus, Peach rosette mosaic virus (PRMV)
Rust: Tranzschelia discolor (Fuckel) Tranzschel and Litv.
Scab: Cladosporium carpophilum Thuem. = Fusicladium carpophilum
(Thuem) Oudem, Teleomorph: Venturia carpophila E. E. Fisher
POTATO (SOLANUM TUBEROSUM L.)
Bacterial wilt (brown rot): Ralstonia solanacearum (Smith 1896)
Yabauuchi, Kosako, Yano, Hotta, and Nishiuchi 1995
Black dot: Colletotrichum coccodes (Wallr.) S. J. Hughes =
C. atramentarium (Berk. and Broome) Taubenhaus
Blackleg and bacterial soft rot: Erwinia carotovora ssp. atroseptica (van
Hall 1902) Dye 1969, E. carotovora ssp. carotovora (Jones 1901)
Bergey, Harrison, Breed, Hammer and Huntoon 1923, E. chrysanthemi
Burkholder, McFadden, and Dimock 1953
Cercospora leaf blotch: Mycovellosiella concors (Casp.) Deighton =
Cercospora concors (Casp.) Sacc., C. solani-tuberosi Thirumalachar.
Early blight: Alternaria solani Sorauer
Fusarium dry rot: F. sambucinum Fuckel, Teleomorph: Gibberella
pulicaris (Fr.:Fr.) Sacc., F. solani (Mart.) Sacc. var. coeruleum (Lib. ex
Sacc.) C. Booth, F. avenaceum (Fr.:Fr.) Sacc., Teleomorph: Gibberella
avenacea R. J. Cooke
Fusarium wilt: F. avenaceum (Fr.:Fr.) Sacc., F. oxysporum Schlechtend.:
Fr., F. solani (Mart.) Sacc., Teleomorph: Nectria haematococca Berk.
and Broome, F. solani (Mart.) Sacc. f. sp. eumartii (C. Carpenter)

W. C. Snyder and H. N. Hans = F. eumartii C. Carpenter
Gangrene: Phoma exigua Desmaz. = P. solanicola Prill. and Delacr.,
P. foveata Foister = P. exigua var. foveata (Foister) Boerema
(de Bary) Whetzel
Late blight: Phytophthora infestans (Mont.) de Bary
Leak: Pythium spp., P. debaryanum Auct. non R. Hesse, P. ultimum Trow
Phoma leaf spot: Phoma andina Turkensteen
Pink eye: Pseudomonas fluorescens Migula
Pink rot: P. cryptogea Pethybr. and Lafferty, P. drechslera Tucker,
P. erythroseptica Pethybr.
Potato Andean latent: genus Tymovirus, Potato Andean latent virus
(APLV)
Potato Andean mottle: genus Comovirus, Potato Andean mottle virus
(APMV)
Potato aucuba mosaic: genus Potexvirus, Potato aucuba mosaic virus
(PAMV)
Potato early dying, Verticillium wilt: Verticillium albo-atrum Reinke and
Berthier, V. dahliae Kleb.
Potato leaf roll mosaic: genus Luteovirus, Potato leaf roll virus (PLRV)
Potato leaf rolling: genus Carlavirus, Potato virus M (PVM)
Potato mild mosaic: genus Potyvirus, Potato virus A (PVA)
Potato mop-top: genus Furovirus, Potato mop-top virus (PMTV)
Potato rugose mosaic: genus Potyvirus, Potato virus Y (PVY, strains O, N,
and C)
Potato spindle tuber: Potato spindle tuber viroid (PSTV)
Potato stem mottle (spraing of tubers): genus Tobravirus, Tobacco rattle
virus (TRV)
Potato virus S: genus Carlavirus, Potato virus S (PVS)
Potato virus T: genus Trichovirus, Potato virus T (PVT)
Potato virus X: genus Potexvirus, Potato virus X (PVX)
Potato yellow vein: genus Bigeminivirus, Potato yellow vein virus (PYVV)
Powdery mildew: Erysiphe cichoracearum DC., Anamorph: Oidium
asteris-punicei Peck
Powdery scab: Spongospora subterranea (Wallr.) Lagerh
Rhizoctonia canker (black scurf): Rhizoctonia solani Khn, Teleomorph:
Ring rot: Clavibacter michiganensis ssp. sepedonicus (Spieckermann and
Kotthoff 1914) Davis, Gillaspie, Vidaver, and Harris 1984
Rosellinia black rot: Rosellinia sp.
Septoria leaf spot: Septoria lycopersici Speg.
Silver scurf: Helminthosporium solani Durieu and Mont. =

Spondylocladium atrovirens (C. Harz) C. Harz ex Sacc.
Skin spot: Polyscytalum pustulans (N. M. Owensand Wakef.) M. B. Ellis
Stem rot (southern blight): Sclerotium rolfsii Sacc., Teleomorph: Athelia
rolfsii (Curzi) Tu and Kimbrough
(TSWV) (family Bunyaviridae)
Wart: Synchytrium endobioticum (Schilberszky) Percival
White mold: Sclerotinia sclerotiorum (Lib.) de Bary, S. minor Jagger =
S. intermedia Ramsey
RICE (ORYZA SATIVA L.)
Aggregate sheath spot: Ceratobasidium oryzae-sativae Gunnell and
Webster, Anamorph: Rhizoctonia oryzae-sativae (Sawada) Mordue
Bacterial blight: Xanthomonas oryzae pv. oryzae (Ishiyama 1922) Swings,
Van den Mooter, Vauterin, Hoste, Gillis, Mew, and Kersters 1990
Bacterial foot rot: Erwinia chrysanthemi Burkholder, McFadden, and
Dimock 1953.
Bacterial leaf streak: Xanthomonas oryzae pv. oryzicola (Ishiyama 1922)
Swings, Van den Mooter, Vauterin, Hoste, Gillis, Mew, and Kersters 1990
Black kernel: Curvularia lunata (Wakk.) Boedijn, Teleomorph:
Cochiobolus lunatus R. R. Nelson and Haasis
Blast: Magnaporthe grisea (Hebert) Barr, Anamorph: Pyricularia grisea
Sacc. = Pyricularia oryzae Cavara
Brown spot: Cochliobolus miyabeanus (Ito and Kuribayashi) Drechs. ex
Dastur, Anamorph: Bipolaris oryzae (Breda de Haan) Shoemaker =
Helminthosporium oryzae Breda de Haan
Crown sheath rot: Gaeumannomyces graminis (Sacc.) Arx and D. Olivier
Downy mildew: Sclerophthora macrospora (Sacc.) Thirumalachar et al.
Eyespot: Drechslera gigantea (Heald and F. A. Wolf) Ito
False smut: Ustilaginoidea virens (Cooke) Takah.
Foot rot (Bakane): Fusarium moniliforme Sheldon, Teleomorph:
Gibberella fujikuroi (Sawada) Ito in Ito and K. Kimura
Giallume: genus Luteovirus, Barley yellow dwarf virus (BYDV)
Grain rot: Burkholderia glumae (Kurita and Tabei 1967) Urakami, ItoYoshida, Araki, Kijima, Suzuki, and Komagata 1994 = Pseudomonas
glumae Kurita and Tabei 1967
Kernel smut: Tilletia barclayana (Bref.) Sacc. and Syd. in Sacc. =
Neovossia horrida (Takah.) Padwick and A. Khan
Leaf scald: Microdochium oryzae (Hashioka and Yokogi) Samuels and

I. C. Hallett = Rhynchosporium oryzae Hashioka and Yokogi
Leaf smut: Entyloma oryzae Syd. and P. Syd.
Narrow brown leaf spot: Cercospora janseana (Racib.) O. Const. =
C. oryzae Miyake, Teleomorph: Sphaerulina oryzina K. Hara
Rice black streak dwarf: genus Fijivirus, Rice black streak dwarf virus
(RBSDV)
Rice dwarf: genus Phytoreovirus, Rice dwarf virus (RDV)
Rice gall dwarf: genus Phytoreovirus, Rice gall dwarf virus (RGDV)
Rice grassy stunt: genus Tenuivirus, Rice grassy stunt virus (RGSV)
Rice hoja blanca: genus Tenuivirus, Rice hoja blanca virus (RHBV)
Rice necrosis mosaic: genus Bymovirus, Rice necrosis mosaic virus (RNMV)
Rice ragged stunt: genus Oryzavirus, Rice ragged stunt virus (RRSV)
Rice stripe: genus Tenuivirus, Rice stripe virus (RStV)
Rice stripe necrosis: genus Furovirus, Rice stripe necrosis virus (RStNV)
Rice transitory yellowing: genus Nucleorhabdovirus, Rice transitory
yellowing virus (RTYV)
Rice yellow dwarf: Phytoplasma
Rice yellow mottle: genus Sobemovirus, Rice yellow mottle virus (RYMV)
Sheath blight: Rhizoctonia solani Khn Teleomorph: Thanatephorus
cucumeris (A.B. Frank) Donk
Sheath brown rot: Pseudomonas fuscovaginae (ex Tanii, Miyajima, and
Akita 1976) Miyajima, Tanii, and Akita 1983
Sheath rot: Sarocladium oryzae (Sawada) W. Gams and D. Hawksworth =
Acrocylindrium oryzae Sawada
Sheath spot: Rhizoctonia oryzae Ryker and Gooch
Stackburn: Alternaria padwickii (Ganguly) M.B. Ellis = Trichoconis
padwickii Ganguly
Stem rot: Magnaporthe salvinii (Cattaneo) R. Krause and Webster,
Synanamorphs: Sclerotium oryzae Cattaneo, Nakataea sigmoidae
(Cavara) K. Hara
Tungro: genus Badnavirus, Rice tungro bacilliform virus (RTBV); genus
Waikavirus, Rice tungro spherical virus (RTSV)
Udbatta: Ephelis oryzae Syd., Teleomorph: Balansia oryzae-sativae
Hashioka
TOMATO (LYCOPERSICON ESCULENTUM MILL.)
Alternaria stem canker: Alternaria alternata (Fr.:Fr.) Keissl. f. sp.
lycopersici Grogan et al.
Anthracnose: Colletotrichum coccodes (Wallr.) S. J. Hughes, C. dematium

(Pers.) Grove, C. gloeosporioides (Penz.) Penz. and Sacc. in Penz.,
Teleomorph: Glomerella cingulata (Stoneman) Spauld. and H. Schrenk
Bacterial canker: Clavibacter michiganensis ssp. michiganensis (Smith
1910) Davis, Gillaspie, Vidaver, and Harris 1984
Bacterial speck: Pseudomonas syringae pv. tomato (Okabe 1933) Young,
Bacterial spot: Xanthomonas vesicatoria (ex Doidge 1920) Vauterin,
Hoste, Kersters, and Swings 1995 = Xanthomonas campestris pv.
vesicatoria (Doidge 1920) Dye 1978
Bacterial stem rot and fruit rot: Erwinia carotovora ssp.carotovora (Jones
1902) Bergey, Harrison, Breed, Hammer, and Huntoon 1923
Bacterial wilt: Ralstonia solanacearum (Smith 1896) Yabauuchi, Kosako,
Yano, Hotta, and Nishiuchi 1995
Black root rot: Thielaviopsis basicola (Berk. and Broome) Ferraris,
Synanamorph: Chalara elegans Nag Raj and Kendrick
Black shoulder: Alternaria alternata (Fr.:Fr.) Keissl.
Buckeye fruit and root rot: Phytophthora capsici Leonian, P. dreshsleri
Tucker, P. nicotianae Breda de Haan var. parasitica (Dastur) G. M.
Waterhouse = P. parasitica Dastur
Cercospora leaf mold: Pseudocercospora fuligena (Roldan) Deighton =
Cercospora fuligena Roldan
Common mosaic of tomato: genus Tobamovirus, Tobacco mosaic virus
(TMV)
Corky root rot: Pyrenochaeta lycopersici R. Schneider and Gerlach
Curly top: genus Hybrigeminivirus, Tomato curly top virus (TCTV)
Didymella stem rot: Didymella lycopersici Kleb.
Early blight: Alternaria solani Sorauer
Fusarium crown and root rot: Fusarium oxysporum Schlechtend.:Fr. f. sp.
radicis-lycopersici W. R. Jarvis and Shoemaker
Fusarium wilt: Fusarium oxysporum Schlechtend.:Fr. f. sp. lycopersici
(Sacc.) W. C. Snyder and H. N. Hans.
Gray leaf spot: Stemphylium botryosum Wallr. f. sp. lycopersici Rotem
et al., S. lycopersici (Enjoji) W. Yamamoto = S. floridanum Hannon
and G. F. Weber, S. solani G. F. Weber
(de Bary) Whetzel
Late blight: Phytophthora infestans (Mont.) de Bary
Leaf mold: Fulvia fulva (Cooke) Cif. = Cladosporium fulvum Cooke
Phoma rot: Phoma destructiva Plowr. (Anamorphic Pleosporaceae)
Pith necrosis: Pseudomonas corrugata (ex Scarlett, Fletcher, Roberts, and

Lelliott 1978) Roberts and Scarlett 1981
Potato virus Y: genus Potyvirus, Potato virus Y (PVY)
Powdery mildew: Oidiopsis sicula Scalia, Teleomorph: Leveillula taurica
(Lv.) G. Arnaud
Pseudo curly top: genus Hybrigeminivirus, Tomato pseudo curly top virus
(TPCTV)
Pythium damping-off and fruit rot: Pythium aphanidermatum (Edson)
Fitzp., P. arrhenomanes Drechs., P. debaryanum Auct. non R. Hesse,
P. myriotylum Drechs., P. ultimum Trow
Rhizoctonia damping-off and fruit rot: Rhizoctonia solani Khn,
Teleomorph: Thanatephorus cucumeris (A. B. Frank) Donk
Rhizopus rot: Rhizopus stolonifer (Ehrenb.:Fr.) Vuill.
Septoria leaf spot: Septoria lycopersici Speg.
Sour rot: Geotrichum candidum Link, Teleomorph: Galactomyces
geotrichum (E. E. Butler and L. J. Petersen) Redhead and Malloch,
G. penicillatum (do Carmo Sousa) Arx
Syringae leaf spot: Pseudomonas syringae pv. syringae van Hall 1902
Target spot: Corynespora cassiicola (Berk. and M. A. Curtis) C. T. Wei
Tomato big bud: Phytoplasma
Tomato bunchy top: Tomato bunchy top viroid
Tomato bushy stunt: genus Tombusvirus, Tomato bushy stunt virus
(TBSV)
Tomato etch: genus Potyvirus, Tobacco etch virus (TEV)
Tomato fern leaf: genus Cucumovirus, Cucumber mosaic virus (CMV)
Tomato mosaic: genus Tobamovirus, Tomato mosaic virus (ToMV)
Tomato mottle: genus Bigeminivirus, Tomato mottle virus (ToMoV)
Tomato necrosis: genus Alfamovirus, Alfalfa mosaic virus (AMV)
Tomato planto macho: Tomato planto macho viroid
(TSWV)
Tomato yellow leaf curl: genus Bigeminivirus, Tomato yellow leaf curl
virus (TYLCV)
Tomato yellow top: Tomato yellow top virus (TYTV)
Kleb.
White mold: Sclerotinia sclerotiorum (Lib.) de Bary, S. minor Jagger
WHEAT (TRITICUM SPP.)

Agropyron mosaic: genus Rymovirus, Agropyron mosaic virus (AgMV)
Alternaria leaf blight: Alternaria triticina Pras. and Prab.
Ascochyta leaf spot: Ascochyta tritici Hori and Enjoji
Aster yellows: Aster yellows phytoplasma
Bacterial leaf blight: Pseudomonas syringae pv. syringae van Hall 1902
(BSMV)
Barley yellow dwarf: genus Luteovirus, Barley yellow dwarf virus (BYDV)
Barley yellow striate mosaic: genus Cytorhabdovirus, Barley yellow
striate mosaic virus (BYSMV)
Basal glume rot: Pseudomonas syringae pv. atrofaciens (McCulloch
Brome mosaic: genus Bromovirus, Brome mosaic virus (BMV)
Cephalosporium stripe: Hymenula cerealis Ellis and Everh. =
Cephalosporium gramineum Nisikado and Ikata in Nisikado et al.
Cereal northern mosaic = barley yellow striate mosaic: genus
Cytorhabdovirus, Northern cereal mosaic virus (NCMV) (family
Rhabdoviridae, Order Mononegavirales)
Cereal tillering: genus Reovirus, Cereal tillering disease virus (CTDV)
Cocksfoot mottle: genus Sobemovirus, Cocksfoot mottle virus (CoMV)
Common bunts: Tilletia tritici (Bjerk.) G. Wint. in Rabenh = Tilletia
caries (DC.) Tul. and C. Tul., T. laevis Khn in Rabenh. = T. foetida
(Wallr.) Liro
Crown rot: Fusarium culmorum (Wm. G. Smith) Sacc., F. graminearum
Schwabe, Teleomorph: Gibberella zeae (Schwein.) Petch, Bipolaris
sorokiniana (Sacc.) Shoemaker
Dilophospora leaf spot and twist: Dilophospora alopecuri (Fr.) Fr.
Downy mildew: Sclerophthora macrospora (Sacc.) Thirum. et al =
Sclerospora macrospora Sacc.
Dwarf bunt: Tilletia controversa Kuhn in Rebenh.
Ergot: Claviceps purpurea (Fr.:Fr.) Tul., Anamorph: Sphacelia segetum Lev.
Eyespot: Pseudocercosoporella herpotrichoides (Fron) Deighton,
Teleomorph: Tapesia yallundae Wallwork and Spooner, T. acuformis
(Pers.) Fuckel
Flag smut: Urocystis agropyri (Preuss) Schroet.

Gray snow mold (= Typhula blight): Typhula incarnata Fr., Typhula
ishikariensis Imai var. idahoensis (Remsb.) Arsvoll and Smith, T.
ishikariensis Imai var. ishikariensis, T. ishikariensis Imai var.
canadensis Smith and Arsvoll
Halo spot: Pseudoseptoria donacis (Pass.) Sutton = Selenophoma donacis
(Pass.) R. Sprague and A. G. Johnson
Herpotrichoides leaf spot: Leptosphaeria herpotrichoides de Not =
Phaeosphaeria herpotrichoides (de Not.) Holm
Karnal bunt: Tilletia indica Mitra = Neovossia indica (Mitra) Mundkur
Leaf and glume blotch: Stagonospora nodorum (Berk.) Castellani and
E. G. Germano = Septoria nodorum (Berk.) Berk. in Berk. and
Broome, Teleomorph: Phaeosphaeria nodorum (E. Muller)
Hedjaroude, Leptosphaeria nodorum Muller
Leaf (brown) rust: Puccinia triticina Eriks. = Puccinia recondita Rob. ex
Desm. f. sp. tritici = Puccinia recondita Rob. ex Desm.
Loose smut: Ustilago tritici (Pers.) Rostr.
Maize dwarf mosaic: genus Potyvirus, Maize dwarf mosaic virus
(MDMV)
Maize streak: genus Monogeminivirus, Maize streak virus (MSV)
Microscopica leaf spot: Leptosphaeria microscopica Karst., Anamorph:
Phaeoseptoria urvilleana (Speg.) Sprague
Phoma spot: Phoma glomerata (Cda.) Wr. and Hochapf., P. insidiosa
Tassi
Pink seed: Erwinia rhapontici (Millard 1924) Burkholder 1948 =
Pectobacterium rhapontici (Millard 1924) Patel and Kulkarni 1951
Fusarium nivale Ces. ex Sacc., Teleomorph: Monographella nivalis
Powdery mildew: Blumeria graminis (DC.) E. O. Speer f. sp. tritici =
Erysiphe graminis DC. Fr. f. sp. tritici
Pythium root rot: P. aphanidermatum (Edson) Fitzp., Pythium
graminicola Subramanian, Pythium arrhenomanes Drechs., Pythium
tardicrescens Vanderpool, P. volutum Vant. and Tru, P. myriotylum
Drechs.
virus (RBSDV)
Rice hoja blanca: genus Tenuivirus, Rice hoja blanca virus (RHBV)
Scab (= head blight): Fusarium graminearum Schwabe, Teleomorph:
Gibberella zeae (Schwein.) Petch, Fusarium culmorum (Wm. G.
Smith) Sacc., F. avenaceum (Fr.) Sacc., Teleomorph: Gibberella

avenaceae Cook
Septoria speckled leaf blotch: Septoria tritici Rob. in Desm.,
Teleomorph: Mycosphaerella graminicola (Feckl.) Sand, Stagonospora
avenae f. sp. triticea T. Johnson
Sharp eyespot: Rhizoctonia cerealis Van der Hoeven, Teleomorph:
Ceratobasidium cereale D. Murray and L. L. Burpe, Rhizoctonia
solani Kuhn, Teleomorph: Thanatephorus cucumeris (A. B. Frank)
Donk
Snow rot: Pythium iwayamai Ito, P. okanoganense Lipps, P. paddicum
Harane
Snow scald: Myriosclerotinia borealis (Bubak and Vleugel) L. M. Kohn =
Sclerotinia borealis Bubak and Vleugel
Speckled snow mold: Typhula idahoensis Remsberg
Spike blight: Rathayibacter tritici (ex Hutchinson 1917) Zgurskaya,
Evtushenko, Akimov, and Kalakoutskii 1993
Spot blotch: Cochliobolus sativus (Ito and Kuribayashi) Drechs. ex
Dastur, Anamorph: Drechslera teres (Sacc.) Shoemaker
Stem rust: Puccinia graminis f. sp. tritici (Pers.) Erikss. et Henn
Stripe (yellow) rust: Puccinia striiformis Westend = Puccinia striiformis
Westend f. sp. tritici
Take-all: Gaeumannomyces graminis (Sacc.) von Arx and Oliver var.
tritici Walker, Gaeumannomyces graminis (Sacc.) von Arx and Oliver
var. graminis, Gaeumnnomyces (Sacc.) von Arx and Oliver var. avenae
(E. M. Turner) Dennis = Ophiobolus graminis Sacc.
Tan spot (= yellow leaf spot): Pyrenophora tritici-repentis (Died.) Drechs.
= Pyrenophora trichostoma (Fr.) Fuckel, Anamorph: Drechslera triticirepentis (Died.) Shoemaker = Helminthosporium tritici-repentis Died.
Tar spot: Phyllachora graminis (Pers.:Fr.) Fekl.
Wheat chlorotic streak: genus Cytorhabdovirus, Wheat chlorotic streak
virus (WCSV)
Wheat dwarf: genus Monogeminivirus, Wheat dwarf virus (WDV)
Wheat soil-borne mosaic: genus Furovirus, Wheat soil-borne mosaic
virus (SBWMV)
Wheat spindle streak mosaic: genus Bymovirus, Wheat spindle streak
mosaic virus (WSSMV)
Wheat streak mosaic: genus Rymovirus, Wheat streak mosaic virus
(WSMV)
Wheat yellow leaf: genus Closterovirus, Wheat yellow leaf virus (WYLV)
Wheat yellow mosaic: genus Bymovirus, Wheat yellow mosaic virus
(WYMV) (family Potyviridae)
White blotch: Bacillus megaterium pv. cerealis Hosford 1982
Winter crown rot (Coprinus snow mold): Coprinus psychromorbidus
Redhead and Traquair
REFERENCES
APSnet (1997). <www.apsnet.org>.
Hansen, J. D. (1985). Common names for plant diseases. Plant Dis, 69:649-676.
Hawksworth, D. L., Kirk, P. M., Sutton, B. C., and Pegler, D. N. (1995). Ainsworth
and Bisbys Dictionary of Fungi. CAB International, U.K.
Schaad, N. W., Vidaver, A. K., Lacy, G. H., Rudolph, K., and Jones, J. B. (2000).
Evaluation of proposed amended names of several pseudomonads and xanthomonads and recommendations. Phytopathology, 90:208-213.
Van Regenmortel, M. H. V. (1999). How to write the names of virus species. Arch
Virol, 144:1041-1042.
Wrobel, W. and Creber, G. (1998). Elseviers Dictionary of Fungi and Fungal Plant
Diseases. Elsevier, Amsterdam, The Netherlands.
Young, J. M., Saddler, G. S., Takikawa, Y., De Boer, S. H., Vauterin, L., Gardan,
L., Gvozdyak, R. I., and Stead, D. E. (1996). Names of plant pathogenic bacteria,
1864-1995. Rev Plant Pathol, 75:721-761.
HOST DEFENSE MECHANISMS
31
CellCell
Wall
WallReinforcement
Reinforcement
Cell walls of host plants offer resistance to fungal and bacterial pathogens by rapidly modifying their structure when pathogens try to penetrate
and disintegrate them. Early induction of cell wall modifications is characteristic of resistant interactions. The possible role of cell walls in host defense mechanisms is described in this chapter.
CELL WALL MODIFICATIONS
A host cell wall offers resistance to pathogen penetration or multiplication due to the interlocking network of macromolecules. The cell wall is in a
dynamic state, and when a fungal or bacterial pathogen tries to penetrate or
disintegrate the cell wall to facilitate its spread, the cell wall reinforces itself
by rapidly modifying its structure. The most common response is formation
of cell wall appositions, which are otherwise called papillae. Papillae comprise a callose matrix, incorporated with pectic materials, cellulose, suberin,
gums, calcium, and silicon. Lignification of papillae occurs. Incorporation
of hydroxyproline-rich glycoprotein (HRGP) strengthens the papillae. Several toxic substances, including phenolics, flavonoids, and H2O2, accumulate in the papillae.
Upon penetration by some fungal pathogens, such as powdery mildew
and rust fungi, another reactive material, the collar, is deposited along the
haustorial neck of fungi within the host cell. In some cases, this collar may
develop to such an extent that the entire haustorium is encased. The collar
develops from papillae and is mostly composed of cellulosic -1,4-glucans
and callose. Callose is a polysaccharide containing a high proportion of 1,3-linked glucose and is a polymer of -1,3-glucans. Uridine diphosphoglucose (UDP-glucose) is converted into a -1,3-glucan, callose, by -1,3glucan synthase (callose synthase). Callose is a minor component of healthy
plant tissues. Plants respond to infection by pathogens with the rapid deposition of callose. The presence of collars is usually associated with poor de-
velopment of the haustoria. The collar may act as a barrier to apoplastic flow
in rust fungi.
The extrahaustorial matrix is another component involved in cell wall reinforcement. In the case of powdery mildew and rust fungi, the haustorium
is surrounded by an extrahaustorial matrix. The matrix may contain lipids,
polysaccharides, glycoproteins, and proteins, but cellulose and pectin are
usually absent.
Papillae formation is also associated with bacterial disease resistance.
When lettuce leaves were inoculated with the incompatible bacterial pathogen Pseudomonas savastanoi pv. phaseoli, an apparent convolution of the
plasma membrane occurred next to bacterial cells. Fibrillar material accumulates between the convoluted membrane, and progressive thickening and
increase in complexity of paramural deposits occur within eight hours after
inoculation. Swelling of the endoplasmic reticulum and an increase in numbers of smooth vesicles (papillae) are observed at sites of paramural deposition (Bestwick et al., 1995).
ROLE OF CELL WALL MODIFICATIONS
IN DISEASE RESISTANCE
Papillae may function as an important resistance mechanism. The role of
papillae in host defense mechanisms has been demonstrated by the following evidence:
1. Papillae are formed abundantly in only resistant/incompatible hostpathogen interactions. In susceptible interactions, fewer and smaller
papillae are formed.
2. When the disease-resistant plants are treated with inhibitors of papilla
formation (such as 2-deoxy-D-glucose), papillae formation is inhibited, and the plants become susceptible to pathogens.
3. When papilla formation is induced by treatments with chemicals such
as chitosan or some plant extracts, more and larger papillae are
formed, and the susceptible plants become resistant.
Earlier papilla initiation is an important factor for papillae-mediated disease resistance. When papilla deposition starts earlier and increases more
rapidly, the plants become more resistant. Restriction of growth of Leptosphaeria maculans to the infected areas in cotyledons of Brassica napus-B.
nigra addition line (LA4+) plants was correlated to reinforcement of cell
wall barriers, including wall apposition, papillae, and vessel plugging, and
the disease resistance was associated with rapidity in plant responses (Roussel
et al., 1999). A low penetration efficiency of fungal pathogens is correlated

with papillae that are formed in advance of penetration pegs. Frequency of
the papilla formation is also important. Treatments such as spraying of water extract of a plant such as Reynoutria sachaliensis induce an increased
frequency of papillae and confer resistance to pathogens (Schneider and
Ullrich, 1994). The size of papillae is also important. Papillae formed in resistant interactions are larger in size than those formed in susceptible interactions. Oversized papillae contribute to disease resistance.
The factors responsible for early induction of papilla formation in resistant interactions have been studied. An aqueous extract from barley seedlings contains the factor that induces papilla formation, called papilla-regulating extract (PRE). When coleoptiles of a susceptible variety are floated
on PRE solution, papillae are initiated much earlier than in the control when
inoculated with the powdery mildew pathogen (Erysiphe graminis). Papillae are initiated before penetration peg initiation in PRE treatments,
while they are initiated after penetration peg initiation in controls (Inoue et
al., 1994). PRE appears to contain potassium phosphate. Potassium phosphate extracted from uninoculated barley leaves induces papilla mediated
resistance against the powdery mildew pathogen in barley. PRE-induced resistance appears to be Ca2+ mediated. Phosphate salts (in the presence of
Ca2+), such as Ca(H2PO4)2, have been shown to induce local and systemic
resistance in many plants. Callose synthesis by callose synthase is activated
by Ca2+.
REFERENCES
Bestwick, C. S., Bennett, M. H., and Mansfield, J. W. (1995). Hrp mutant of Pseudomonas syringae pv. phaseolicola induces cell wall alterations but not membrane damage leading to the hypersensitive reaction in lettuce. Plant Physiol,
108:503-516.
Inoue, S., Aist, J. R., and Macko, V. (1994). Earlier papilla formation and resistance
to barley powdery induced by a papilla-regulating extract (PRE). Physiol Mol
Plant Pathol., 44:433-440.
Roussel, S., Nicole, M., Lopez, F., Renard, M., Chevre, A. M., and Brun, H. (1999).
Cytological investigation of resistance to Leptosphaeria maculans conferred to
Brassica napus by introgressions originating from B. juncea or B. nigra B genome. Phytopathology, 89:1200-1213.
Schneider, S. and Ullrich, W. R. (1994). Differential induction of resistance and enhanced enzyme activities in cucumber and tobacco caused by treatments with
various abiotic and biotic inducers. Physiol Mol Plant Pathol, 45:291-304.
32
Hydroxyproline-Rich
Hydroxyproline-Rich Glycoproteins
Glycoproteins
Plant cell walls contain different types of hydroxyproline-rich glycoproteins. These proteins show extensive modifications when pathogens try
to penetrate the plant cell wall, and these modified HRGPs offer resistance
to pathogens. The role of HRGPs in disease resistance is described in this
chapter.
TYPES OF CELL WALL PROTEINS
A plant cell wall contains up to 10 percent protein. Cell wall protein is exceptionally rich in hydroxyproline (Hyp). There are four types of hydroxyproline-rich protein classes.
1. Extensins: Extensins are rich in hydroxyproline and serine (Ser) and
usually contain the repeating pentapeptide motif Ser-Hyp4. Other
commonly found amino acids are valine, tyrosine, lysine, and histidine.
2. Proline (PRO)-rich proteins (PRPs): All PRPs are characterized by the
repeating occurrence of PRO-PRO repeats. They contain approximately equimolar quantities of proline and hydroxyproline.
3. Lectins: Hydroxyproline and arabinose are major constituents of
lectins. The serine-hydroxyproline-rich glycopeptide domain of lectins
bears a striking biochemical resemblance to the extensins.
4. Arabinogalactan proteins (AGPs): They are HRGPs that are highly
glycosylated. The protein moiety of AGPs is typically rich in hydroxyproline, serine, alanine (Ala), threonine, and glycine. They contain
Ala-Hyp repeats.
ROLE OF HRGPS IN DISEASE RESISTANCE
HRGPs accumulate in infected tissues, particularly in cell wall appositions (papillae). Accumulation of HRGPs in plant cell walls is one of the
earliest changes observed in the cell walls of leaves inoculated with incom-
patible pathogens (resistant interactions) (Bestwick et al., 1995; Davies

et al., 1997). Several new HRGPs have been shown to be synthesized in resistant host-pathogen interactions. For example, Davies and colleagues
(1997) reported that when turnip leaves were inoculated with an incompatible strain of Xanthomonas campestris pv. raphani, two new HRGPs, gp160
and gpS, were induced. In a compatible interaction with an X. campestris
pv. campestris strain, no induction of these glycoproteins was observed.
The newly induced gpS comprised three distinct fractions, gpS-1, gpS-2,
and gpS-3. The gpS-3 fraction may be an HRGP of the extensin family,
while gpS-1 and gpS-2 may be an arabinogalactan protein HRGP of another
family. The new HRGPs may prevent penetration of the cell wall by pathogens.
In addition to the synthesis of new HRGPs, HRGPs in plant cell walls become insolubilized in resistant interactions. The insolubilization may be
caused by linking HRGPs by isodityrosine residues and by a diphenyl ether
linkage, and this process may be due to the action of peroxidase requiring
H2O2 generated via the oxidative burst (Brisson et al., 1994). H2O2 treatment usually induces insolubilization of HRGPs, similar to fungal elicitor
treatment. Simultaneous addition of catalase or ascorbate inhibits the effects of elicitor or H2O2 (Bradley et al., 1992), which suggests that the
elicitor may stimulate H2O2-dependent cross-linking of preexisting, soluble
forms of HRGPs. The cross-linking of the HRGPs in response to elicitor
treatment is initiated within five minutes, and the response is completed
within 20 to 30 minutes (Bradley et al., 1992). The burst of H2O2 normally
occurs within one to two minutes of elicitor treatment in plant cells (Apostol
et al., 1989) and provides substrate for peroxidase-mediated oxidative
cross-linking of HRGPs. The insolubilized HRGPs may lead to an impenetrable cell wall barrier, impeding pathogen infection.
In many host-pathogen interactions, HRGPs are cross-linked with different cell wall constituents. In the resistant interactions, HRGPs are crosslinked with pectin, lignin, and other phenolics (Bestwick et al., 1995).
HRGPs may act as matrices for lignification. When fungal pathogens invade
host tissues, HRGPs accumulate and provide a template for the subsequent
deposition of lignin. Lignified papillae containing HRGP offer resistance to
fungal penetration (Benhamou et al., 1990). The increased cross-linking of
HRGPs leads to strengthening of the cell wall to localize pathogens. Cell
walls, which undergo ultra-rapid HRGP cross-linking, due to pathogen invasion, are tougher than cell walls of uninfected healthy tissues (Showalter,
1993). All of these changes in HRGPs occur simultaneously and make the
cell walls impermeable to pathogens.
Some HRGPs, lectins, are known to agglutinate pathogens, specifically

bacterial cells (Leach et al., 1982). Agglutination may result in immobilization of bacterial pathogens in the cell surface. This would contribute to disease resistance.
REFERENCES
Apostol, I., Heinstein, P. F., and Low, P. S. (1989). Rapid stimulation of an oxidative burst during elicitation of cultured plant cells. Plant Physiol, 90:109-116.
Benhamou, N., Chamberland, H., and Pauze, F. J. (1990). Implications of pectic
components in cell surface interactions between tomato root cells and Fusarium
oxysporum f. sp. Radicis-lycopersici: A cytochemical study by means of a lectin
with polygalacturonic acid-binding specificity. Plant Physiol, 92:995-1003.
108:503-516.
Bradley, D. J., Kjellbom, P., and Lamb, C. J. (1992). Elicitor- and wound-induced
oxidative cross-linking of a proline-rich plant cell wall protein: A rapid defense
response. Cell, 70:21-30.
Brisson, L. F., Tenhaken, R., and Lamb, C. J. (1994). Functions of oxidative crosslinking of cell wall structural proteins in plant disease resistance. Plant Cell,
6:1703-1712.
Davies, H. A., Daniels, M. J., and Dow, J. M. (1997). Induction of extracellular matrix glycoproteins in Brassica petioles by wounding and in response to Xanthomonas campestris. Mol Plant-Microbe Interact, 10:812-820.
Leach, J. E., Centrell, M. A., and Sequeira, L. (1982). A hydroxyproline-rich bacterial agglutinin from potato: Extraction, purification, and characterization. Plant
Physiol, 70:1353-1358.
Showalter, A. W. (1993). Structure and function of plant cell wall proteins. Plant
Cell, 5:9-23.
33
Lignin
Lignin
Lignin is a complex polymer built from phenolic acids, which are reduced to the corresponding alcohols. Rapid lignification of plant cell walls
appears to be an important host defense mechanism. The role of lignin in
bacterial and fungal disease resistance is described in this chapter.
BIOSYNTHESIS OF LIGNIN
Lignin is a complex polymer deposited in secondary cell walls. Hydroxyproline-rich glycoprotein is known to be a matrix for deposition of lignin.
Lignin is a three-dimensional polymer built from three monomers called
monolignols. The three building units are p-coumaryl, coniferyl, and sinapyl
alcohols, which are polymerized in lignin via free radical production.
Lignin is formed by the random condensation of phenylpropanoid units.
Lignin monomers are produced from phenylalanine by a branch of phenylpropanoid metabolism. The first step of the phenylpropanoid pathway is the
deamination of phenylalanine by phenylalanine ammonia-lyase (PAL) to
yield trans-cinnamic acid. Hydroxylation of cinnamic acid by cinnamic
acid-4-hydroxylase (CA4H) produces p-coumaric acid. Further hydroxylation by p-coumaric hydroxylase (CH) results in the synthesis of caffeic
acid or 5-hydroxyferulic acid. Methylation of these acids by O-methyl
transferases (OMT) produces ferulic and sinapic acids, respectively. The
three acids (p-coumaric, ferulic, and sinapic acids) are coupled to CoA and
reduced to the corresponding alcohols. The end products of this pathway are
the three monolignols, namely p-coumaryl, coniferyl, and sinapyl alcohols
(Vidhyasekaran, 1988). Polymerization of these monolignols is catalyzed
by peroxidases. Plants possess a number of different peroxidase isozymes.
Many of them have been found to be localized to cell walls. Cell wall-bound
peroxidases fall into two subgroups, the anionic and cationic. Both the
acidic and basic peroxidases have been found to be located in plant cell
walls, and both anionic peroxidases and cationic peroxidases are involved in
lignification. Cell wall-bound peroxidases are involved not only in the oxidative polymerization of hydroxylated cinnamyl alcohols but also in the
generation of hydrogen peroxide necessary for lignification. The polymerization of the three cinnamyl alcohols is mediated by the peroxidase-H2O2
system.
ROLE OF LIGNIN IN DISEASE RESISTANCE

Deposition of lignin in plant cell walls has been observed in many plantpathogen interactions. Rapid lignification appears to be an important defense mechanism in plants. Lignin content increased in wheat leaves inoculated with an avirulent strain of Puccinia recondita f. sp. tritici, but no such
increase was observed in leaves inoculated with a virulent strain (Southerton
and Deverall, 1990). Lignification was observed in both phloem and xylem
of infected cassava plants resistant to Xanthomonas axonopodis pv. manihotis. This reaction was observed at a higher intensity in resistant plants
than in susceptible plants (Kpemoua et al., 1996). When rice leaves were inoculated with incompatible strain of X. oryzae pv. oryzae, lignin-like compounds accumulated in rice leaves. Similar accumulation of lignin is not
seen in a compatible interaction (Reimers and Leach, 1991). In resistant or
incompatible interactions between rice and X. oryzae pv. oryzae, increases
in the activities of the three intercellular peroxidases have been correlated
with the accumulation of lignin-like compounds and reduction in bacterial
multiplication in the leaves (Guo et al., 1993). When primary leaves of Red
Mexican bean plants were inoculated with P. savastanoi pv. phaseolicola,
lignin deposition was observed in the inoculated leaf area in the incompatible interaction but not in the compatible interaction (Milosevic and Slusarenko, 1996). Peroxidase activity was also located in the same zone in the
incompatible interaction. The increase in peroxidase activity in the incompatible interaction could largely be attributed to the appearance of a novel
anionic peroxidase from 18 hours after inoculation onward (Milosevic and
Slusarenko, 1996). The novel peroxidase may be involved in the lignification observed in the bacteria-inoculated zone (Milosevic and Slusarenko,
1996).
Lignification in cell walls may serve as a barrier for penetration by fungal
pathogens. Cell wall strengthening due to deposition of lignin was an inducible defense mechanism of banana roots against Fusarium oxysporum f. sp.
cubense (De Ascensao and Dubery, 2000). Although lignification of plant
cell walls has been observed in many host-bacteria resistance reactions, lignification may not serve as a physical barrier against bacterial pathogens,
because bacterial plant pathogens do not penetrate host cells. However, lig-
nified materials may prevent bacterial spread by blocking movement between the epithem and xylem vessels. Reactive compounds associated with
the process of lignification may also inhibit pathogen growth (Vidhyasekaran et al., 2001).
REFERENCES
De Ascensao, A. R. D. C. F. and Dubery, L. A. (2000). Panama disease: Cell wall reinforcement in banana roots in response to elicitors from Fusarium oxysporum f.
sp. cubense race four. Phytopathology, 90:1173-1180.
Guo, A., Reimers, P. J., and Leach, J. E. (1993). Effect of light on compatible interaction between Xanthomonas oryzae pv. oryzae and rice. Physiol Mol Plant
Pathol, 42:413-425.
Kpemoua, K., Boher, B., Nicole, M., Calatayud, P., and Geiger, J. P. (1996).
Cytochemistry of defense responses in cassava infected by Xanthomonas campestris pv. manihotis. Canad J Microbiol, 42:1131-1143.
Milosevic, N. and Slusarenko, A. J. (1996). Active oxygen metabolism and lignification in the hypersensitive response in bean. Physiol Mol Plant Pathol, 49:143158.
Reimers, P. J. and Leach, J. E. (1991). Race-specific resistance to Xanthomonas
oryzae pv. oryzae conferred by bacterial blight resistance gene Xa-10 in rice
Oryza sativa involves accumulation of a lignin-like substance in host tissues.
Southerton, S. G. and Deverall, B. J. (1990). Histochemical and chemical evidence
for lignin accumulation during the expression of resistance to leaf rust fungi in
wheat. Physiol Plant Pathol, 36:483-494.
Vidhyasekaran, P. (1988). Physiology of Disease Resistance in Plants, Volume I.
CRC Press, Boca Raton, FL.
Vidhyasekaran, P., Kamala, N., Ramanathan, A., Rajappan, K., Paranidharan, V.,
and Velazhahan, R. (2001). Induction of systemic resistance by Pseudomonas
fluorescens Pf1 against Xanthomonas oryzae pv. oryzae in rice leaves. Phytoparasitica, 29:155-166.
34
Pathogenesis-Related
Pathogenesis-Related Proteins
Proteins
Several new proteins appear in plants infected with pathogens, and these
are called pathogenesis-related (PR) proteins. They are induced more in resistant interactions. The molecular mechanism of transcription of PR genes,
the signals involved in induction of these genes, and the possible role of PR
proteins in disease resistance are discussed in this chapter.
DEFINITION
When plants are infected by pathogens, a number of genes encoding for
proteins are transcriptionally activated and new proteins are synthesized.
These proteins are called pathogenesis-related proteins. PR proteins have
been defined as proteins encoded by the host plants but induced only in
pathological situations. These PR proteins are induced by fungal, bacterial,
viral, and viroid pathogens. In addition to these pathogens, insect pests,
nematodes, and endophytic saprophytic bacteria, such as some strains of
Pseudomonas fluorescens, induce PR proteins.
Some products from these living organisms also induce PR proteins.
Elicitors isolated from fungi and bacteria induce PR proteins in plants.
Toxins isolated from fungi, and pectic enzymes, cellulases, and xylanases
isolated from fungi and bacteria induce PR proteins. Endogenous elicitors
isolated from plant cell walls (oligogalacturonates) have been shown to induce PR proteins in several plants. Several abiotic compounds are also
known to induce PR proteins. Heavy metals, polyacrylic acid, mannitol, salicylic acid, methyl salicylate, and methyl jasmonate/jasmonic acid induce
synthesis of PR proteins. Several plant activators, such as 2,6-dichloroisonicotinic acid (INA) and benzothiadiazole (BTH) (Latunde-Dada and
Lucas, 2001) are known to induce PR proteins. Several growth regulators,
such as ethylene, kinetin, indoleacetic acid, and abscisic acid, induce PR proteins in various plants. Several environmental factors also induce PR proteins.
Temperature, light, ozone, and ultraviolet rays have been shown to influence induction of PR proteins. Osmotic stress induces PR proteins in some
plants. Any injury or mechanical wounding induces several PR proteins.
Because PR proteins can be induced by several stresses besides pathogens, these proteins should be redefined as plant proteins that are induced
by various types of pathogens as well as stress conditions (Van Loon et al.,
1994). However, even this definition needs modifications, because several
PR proteins have been detected in healthy tissues without any stress in many
plants. In some plants, PR proteins, which appear in leaves only after a
stress, constitutively occur in other parts of plants, including seeds, flowers,
and roots. Many PR proteins appear constitutively in cultured plant cells.
Such proteins, which are induced in one organ of the plant but constitutively
present in other parts of the plant, are also considered to be PR proteins.
Some PR proteins that are induced proteins in some varieties occur constitutively in other varieties. Even in the same plant, PR proteins appear in lower
old leaves without any stress while these proteins could be detected in
young leaves only after inoculation with pathogens.
Hence, it was suggested that to be labeled a PR protein, the protein must
be expressed upon infection in any one organ of the plant, and at least in any
one variety of the plant. However, even PR proteins that could not be detected in unstressed healthy plants by polyacrylamide gel electrophoresis
(PAGE) could be detected when Western blot analyses were made using antisera or when cDNA probes were used. This indicates PR proteins may be
present in uninfected tissues in amounts too small for detection on gels by
general protein stains. Hence, PR proteins are defined as proteins that are
readily detected in infected tissues but not in uninfected ones (Van Loon,
1999). Some proteins are induced exclusively during susceptible interaction, and such proteins are not induced in resistant interactions. These proteins are not considered to be PR proteins (Van Loon, 1999). PR proteins
should be induced in resistant/incompatible interactions, irrespective of
whether they are induced in susceptible/compatible interactions. PR proteins are normally detected in leaves, but in some plants, they are detected
in roots, tubers, stems, petioles, and germinating seeds (Vidhyasekaran,
2002).
Some proteins in healthy, unstressed plant tissues show an amino acid
(nucleotide) sequence similar to already characterized PR proteins. They
may also have enzyme activity similar to some PR proteins. However, they
are never induced by pathogens. Such proteins are called PR-like proteins
(Van Loon et al., 1994).
CLASSIFICATION OF PR PROTEINS
Several PR proteins occur in plants. PR proteins are classified on the basis of their nucleotide sequence or predicted sequence of amino acids,
serological relationship, and/or enzymatic or biological activity (Van Loon
et al., 1994). Serological relationship or enzymatic activity or biological activity alone is not taken as the criteria for classification of PR proteins; they
are considered to be additional characteristics for classification. The structure of PR proteins is the most important factor for classifying PR proteins
into families. PR proteins have been classified into fourteen families based
on shared sequence homology. The isoelectric points of PR proteins are
considered to classify some PR proteins into subclasses (Koiwa et al., 1994).
PR-1 Proteins
This group of PR proteins has been detected widely in the plant kingdom.
Both acidic and basic isoforms (differentiated on the basis of isoelectric
points of the PR proteins) of PR-1 proteins have been detected. In tobacco
alone, ten PR-1 proteins, PR-1a, PR-1b, PR-1c, PR-1d, PR-1e, PR-1f, PR1g, PR-1h, PRB-1a, and PRB-1b, have been detected.
PR-2 Proteins
This family of PR proteins shows -1,3-glucanase activity. -1,3-Glucanases (glucan endo-1,3- -glucosidases) catalyze endo-type hydrolytic
cleavage of the 1,3- -D-glucosidic linkages in -1,3-glucans. -1,3-Glucan,
the substrate for the enzyme -1,3-glucanase, is widespread in plant tissues
and is associated with the formation of callose, leaf and stem hairs, root
hairs, pollen grains, ovules, and wound parenchyma cells. As such, endo 1,3-glucanases are abundant proteins widely distributed in plant species.
Constitutive expression of several -1,3-glucanases in healthy plants has
been reported. These enzymes accumulate in normal healthy plants during
pollen germination, fertilization, seed maturation, and germination. However, some -1,3-glucanases have been shown to be induced by pathogens,
and such enzymes are considered to be PR proteins. Thus, all -1,3-glucanases are not PR proteins; only specific -1,3-glucanases are recognized as
PR-2 proteins. PR-2 -1,3-glucanase exists in multiple forms in a number of
plant species. In tobacco, PR-2 proteins are classified into three structural
classes based on amino acid sequence identity. The class I -1,3-glucanases
are basic proteins localized in the cell vacuole. These include PR-2e of tobacco (Nicotiana tabacum) PR proteins, and Gn1 and Gn2 of Nicotiana
plumbaginifolia. The class II -1,3-glucanases are acidic proteins secreted

into the extracellular space. These include tobacco PR-2a (PR-2), PR-2b
(PR-N), and PR-2c (PR-O). The class III -1,3-glucanases are also acidic
proteins which include PR-2d (= PR-Q') (Leubner-Metzger and Meins,
1999).
PR-3 Proteins
PR-3 proteins show chitinase ( -1,4-glucosaminidase) activity. Chitinases hydrolyze the -glycosidic bond at the reducing end of glucosaminides, which can be parts of various polymers, such as chitin, chitosan, or
peptidoglycan (from bacterial cells) (Neuhaus, 1999). Several chitinases
have been reported in almost all plant species studied. Plant chitinases have
been classified into seven classes based on sequence homology and the
presence or absence of a chitin-binding domain (CBD). Class I contains a
CBD, while class II lacks the CBD. Class III chitinases are structurally different from other chitinases. Class IV chitinases differ from class I by several internal deletions within both chitin-binding and catalytic domains
(loops 1, 3, and 4). Class V chitinases contain two CBDs. Class VI chitinases are characterized by the presence of a truncated CBD and a long
proline-rich spacer. Class VII possesses a catalytic domain homologous to
class IV but lacks the CBD (Neuhaus, 1999). In addition to these seven
classes, two other classes of plant chitinases have also been identified. The
first one is a new type of chitinase with the most related sequences belonging to bacterial chitinases (Melchers et al., 1994), and the other class of
chitinases constitutes hevein and Win proteins, which contain a CBD, and
PR-4 proteins, which lack the CBD (Linthorst et al., 1991).
PR-3 proteins consist of chitinases belonging to class I through class VII,
not including class III (Van Loon et al., 1994). Class III proteins, which are
structurally very different from other classes, are considered to be a separate
family of PR proteins, called PR-8. The chitinases, which are similar to bacterial chitinases, have been grouped as another family, called PR-11. The
PR-11 proteins consist of class I type chitinases. The other group of
chitinases similar to hevein and Win proteins are given a separate family
number, PR-4, which consists of class I- and class II-type chitinases (Van
Loon, 1999). Several chitinases accumulate in plant tissues infected with
pathogens. As many as ten chitinases have been shown to accumulate in
pepper (Lee and Hwang, 1996) and Norway spruce (Sharma et al., 1993) after infection.
PR-4 Proteins
Two classes of the PR-4 family have been reported. Class I includes
hevein of rubber tree and Win protein of potato. The class II PR-4 protein
constitutes tobacco PR-4 as the typical member of PR-4 proteins. One of the
tobacco PR-4 proteins, CBP20, has been found to have chitinase activity,
and the PR-4 family has been included in a chitinase nomenclature.
PR-5 Proteins
The PR-5 group of proteins occurs widely in the plant kingdom. PR-5
proteins have a close resemblance to a sweet-tasting protein, thaumatin,
which occurs in the fruit of the West African shrub Thaumatococcus
danielli. Hence, these proteins are also called thaumatin-like proteins (TLPs).
The basic PR-5 proteins are identical to osmotin, the salt stress protein detected in tobacco and hence, they are called osmotins. Three subclasses of
PR-5 proteins have been recognized in tobacco based on their isoelectric
points: the basic forms (osmotins), neutral forms (osmotin-like proteins,
OLPs), and acidic (PR-S) proteins (Koiwa et al., 1994).
Several PR-5 proteins have been detected in many parts of healthy plants.
They are induced in some plant tissues only after infection with pathogens
(Ruiz-Medrano et al., 1992), and that is why they are called PR proteins.
However, some of the PR-5-type proteins detected in floral parts in tobacco
could not be detected in any other part of the plants even after infection. A
flower-specific gene encoding an osmotin-like protein has been identified in
tomato. No expression of the gene was detected in vegetative organs. The
protein has 30 to 32 percent amino acid sequence identity to pathogenesisrelated osmotins (Chen et al., 1996). However, it cannot be called a PR protein.
The function of PR-5 proteins is not yet known. PR-5 proteins alter permeability of fungal membranes. The PR-5 protein of tobacco (PR-R = PR-S)
has identity to a maize-trypsin/ -amylase inhibitor. A PR-5 protein from
bean cultivar Pinto, PR-4d, shows -1,3-glucanase activity. Another PR
protein from bean cultivar Saxa, which shows resemblance to tobacco PR-5
protein, Saxa PR-4d, possesses chitinase activity (for more details, see
Vidhyasekaran, 2002).
PR-6 Proteins
Some proteinase (protease) inhibitor proteins are considered to be PR-6
proteins. Proteinase inhibitor proteins commonly occur in plants (Vidhyase-
karan, 1997), and some of them are induced by pathogens. Proteinase inhibitors are generally categorized according to the class of proteinases they inhibit (Koiwa et al., 1997). Four types of proteinases have been identified as
serine, cysteine, aspartic, or metallo proteinases based on the active amino
acid in the reaction center. Serine proteinase inhibitors which inhibit trypsin
and chymotrypsin contain several families: Kunitz family (Soybean trypsin
inhibitor family), Bowman-Birk family, Barley trypsin inhibitor family, Potato inhibitor I family, Potato inhibitor II family, Squash inhibitor family,
Ragi I-2/maize trypsin inhibitor family, and Serpin family. Cysteine proteinase inhibitors (phytocystatins) inhibit papain and cathepsin B, H, and L.
Aspartic proteinase inhibitors inhibit cathepsin D, and metallo-proteinase
inhibitors inhibit papain and cathepsin B, H, and L (Koiwa et al., 1997). Tomato inhibitor I (belonging to the Potato inhibitor I family) is the type member of the PR-6 protein family. Tomato inhibitor II is also commonly induced in tomato due to stresses.
PR-7 Proteins
PR-7 proteins show endoproteinase activity. A PR-7 protein, P69, has
been detected in tomato infected by the citrus exocortis viroid. Tornero and
colleagues (1997) identified P69B, a second member of the family of plant
proteinases induced during the response of tomato plants to pathogen attack. P69B represents a new plant subtilisin-like proteinase based on amino
acid sequence conservation and structural organization (Tornero et al.,
1997).
PR-8 Proteins
PR-8 proteins show class III chitinase activity possessing lysozyme activity. These chitinases differ from other chitinases in sequence and/or substrate preference (Van Loon et al., 1994). These PR proteins show structural
homology to a bifunctional lysozyme/chitinase from Parthenocissus quinquifolia. Both acidic and basic forms of PR-8 proteins have been reported.
PR-9 Proteins
PR-9 proteins show peroxidase activity. However all peroxidases are not
considered to be PR-9 proteins, since most of them are constitutively expressed and are not induced during pathogenesis. Over 60 peroxidase genes
from diverse plant species have been isolated and characterized, including
multiple genes from individual species. Only some of the peroxidase genes
are induced during pathogen stress. A lignin-forming peroxidase from tobacco is the type member of the PR-9 protein family.
PR-10 Proteins
PR-10 proteins include intracellular defense-related proteins that have a
ribonuclease-like structure. The PR-10 family includes parsley PR1,
STH-2 in potato, asparagus AoPR1, pea pI49, and bean pvPR1 and pvPR2.
In rice, a PR-10 protein, PBZ1, has been identified (Midoh and Iwata,
1996). This PR protein is induced by Pyricularia oryzae and probenazole.
All PR-10 proteins are acidic, and in all these proteins a signal peptide was
absent, suggesting that this family of proteins is intracellular. The PR-10
gene family may encode ribonucleases.
PR-11 Proteins
PR-11 proteins also show chitinase activity. However, they are distinctly
different from other previously described chitinases. The most related sequences of these proteins belong to bacterial chitinases.
PR-12 Proteins (Defensins)
Defensins are a family of small (about 5 kDa), usually basic, peptides,
which are rich in disulfide-linked cysteine residues (de Silva, Conceicao, and
Broekaert, 1999). Defensins are also considered to be a novel group of
thionins, which are called -thionins. However, thionins and defensins are
structurally unrelated. Terras and colleagues (1995) introduced the term
defensins for this group of peptides, based on the structural and functional
similarities with insect defensins. All defensins ( -thionins) are less than 50
amino acids in length and contain eight cysteine residues. The defensins
have been classified as PR-12 proteins (Van Loon and Van Strien, 1999).
Two radish proteins, Rs-AFP1 and Rs-AFP2, are type members of this family and are induced in radish after infection.
PR-13 Proteins (Thionins)
Thionins are small (5 kDa), basic, cysteine-rich proteins commonly
found in seeds, roots, and leaves of plants. Although thionins are constitutively expressed in seeds and roots, some leaf thionins are found to be induced during pathogenesis. Those induced thionins are considered to be
PR-13 proteins (Van Loon, 1999).
PR-14 Proteins (Lipid Transfer Proteins)

Lipid transfer proteins (LTPs) stimulate the transfer of a broad range of
lipids between membranes in vitro. They are generally secreted and externally associated with the cell wall. They may be involved in the secretion or
deposition of extracellular lipophilic materials such as cutin or wax. LTPs
are distributed at high concentrations in the epidermis of exposed surfaces
and in the vascular tissues. Expression of LTP genes has been shown to be
induced well above basal levels in some plant-pathogen interactions (Garcia-Olmedo et al., 1995). Some LTPs have been considered to be PR-14 proteins (Van Loon and Van Strien, 1999).
Other PR Proteins
In addition to these characterized PR protein classes, some other unclassified proteins are also considered to be PR proteins. Ribosome-inactivating
proteins (RIPs) show specific RNA-N-glycosidase activity, which inactivates ribosomes from phylogenetically distant species including animals
and microorganisms. Some RIPs are induced in the leaves of some plants in
response to pathogens. JIP60, the 60 kDa protein of barley, is induced in
leaves due to pathogen stress and is type member of this family. Some
chitosanases have been identified as PR proteins. Chitosanases act on
chitosan (poly 1-4 - -D-glucosamine, the deacetylated chitin) without any
activity on chitin, and they differ from chitinases by their molecular weight
(10 to 24 kDa) and substrate specificity. No chitosanase activity was detected in uninfected roots of Norway spruce (Picea abies), but three chitosanases were induced after a fungal infection (Sharma et al., 1993). A new
glycine-rich protein (GRP) was induced in tobacco infected with tobacco
mosaic virus (Hooft van Huijsduijnen et al., 1986). Newly induced enzymes
involved in synthesis of phytoalexins are also considered to be PR proteins
(Van Loon et al., 1994).
ROLE OF PR PROTEINS IN DISEASE RESISTANCE
The actual role of PR proteins is still not known. However, many of these
PR proteins are always associated with resistant interactions. The possible
mechanism of inducing resistance by individual PR proteins is discussed in
this section.
PR-1 Proteins
PR-1 proteins have not been shown to inhibit fungal or bacterial growth.
However, they are found to be associated with host cell wall outgrowths and
papillae. PR-1 proteins may restrict the development of pathogens by host
cell wall modifications (Benhamou et al., 1991).
PR-2 Proteins
PR-2 proteins show -1,3-glucanase activity. Fungal cell walls contain
-1,3-glucans. The PR-2 proteins have been shown to lyse the fungal cell
walls. Antiviral action of tobacco -1,3-glucanase (gp35) has also been reported (Edelbaum et al., 1991). -1,3-Glucan is an elicitor, and PR-2 proteins release this elicitor from fungal cell walls by its enzymatic action. The
elicitor induces synthesis of antimicrobial compounds such as phytoalexins,
phenolics, lignins, and other toxic PR proteins.
PR-3 Proteins
PR-3 proteins show chitinase activity. Cell walls of most fungal pathogens
contain chitin. PR-3 proteins act on fungal cell walls and lyse them.
Chitinase inhibits growth of fungi even at 1 to 2 g/ml concentrations. A
transgenic sorghum expressing high levels of chitinase exhibited less stalk
rot development when exposed to conidia of Fusarium thapsinum (Waniska
et al., 2001). Chitin is another important elicitor of defense mechanisms,
and PR-3 proteins release the chitin elicitor molecules from the fungal cell
wall. The elicitor, by inducing antimicrobial compounds, may induce resistance.
PR-4 Proteins
Members of the PR-4 group of proteins are also chitinases. A PR-4 class
I protein from tobacco has been shown to exhibit antifungal activity toward
Fusarium solani, causing lysis of germ tubes and inhibiting fungal growth
(Ponstein et al., 1994).
PR-5 Proteins
Many PR-5 proteins have been shown to have high antifungal activity
(Fagoaga et al., 2001). Some of them caused leakage of cytoplasmic material from the fungi and caused hyphal rupture. AP24 from tobacco and
NP24 from tomato cause lysis of sporangia of Phytophthora infestans. PR-S

from tobacco inhibits hyphal growth of fungi. Osmotin from tobacco causes
fungal spore lysis, inhibition of spore germination, reduced germling viability, and damage to membrane permeability. It induces apoptosis in fungi
(Narasimhan et al., 2001).
PR-6 Proteins
PR-6 proteins are proteinase inhibitors. Several bacterial and fungal
pathogens secrete proteinases. Proteinase-deficient mutants show considerable loss of virulence. Proteinase activity of pathogens is reduced in plants
overexpressing PR-6 proteins, and these plants show resistance to pathogens. It suggests that PR-6 proteins may inhibit proteinases of pathogens
and confer resistance. PR-6 proteins inhibit only fungal and bacterial proteinases and not plant proteinases.
PR-7 Proteins
PR-7 proteins show proteinase activity. They inhibit fungal growth,
probably degrading proteins of pathogens.
PR-8 Proteins
These proteins show chitinase and lysozyme activity. Bacterial cell walls
contain peptidoglycan, which comprises alternate -(1,4)-linked N-acetylmuramic acid residues. Peptidoglycan is hydrolyzed by lysozyme. PR-8
proteins possess lysozyme activity. In many plants, bacterial disease resistance has been shown to be due to increased synthesis of chitinases. PR-8
proteins also show antifungal action.
PR-9 Proteins
PR-9 proteins possess peroxidase activity. They are involved in lignification. They are also involved in cross-linking of extensin monomers, polysaccharide cross-linking, and suberization (Venere et al., 1993). PR-9 proteins may enhance resistance by the construction of a cell wall barrier,
which may impede bacterial pathogen ingress, spread, and development of
fungal pathogens in the apoplast.
PR-10 Proteins
PR-10 proteins contribute for disease resistance; however, their antimicrobial activity has not been established.
PR-11 Proteins
PR-11 proteins show chitinase activity and display antifungal activity in
vitro (Melchers et al., 1994; Ohl et al., 1994).
PR-12 Proteins (Defensins)
PR-12 proteins show antibacterial activity. A potato tuber defensin inhibits Ralstonia solanacearum and Clavibacter michiganensis (Moreno et al.,
1994).
PR-13 Proteins
PR-13 proteins (thionins) are highly toxic to both fungal and bacterial
pathogens. Thionins act on cell membranes. The toxicity is exerted by a direct, detergent-like interaction with the lipid bilayers of biological membranes (Bohlmann, 1994). Thionins may inhibit certain enzymes (such as
cytochrome c) of bacteria and inhibit bacterial growth.
PR-14 Proteins
Lipid transfer proteins may be involved in deposition of extracellular
lipophilic materials such as cutin wax in the plant cell wall. The modified
cell walls may contribute to disease resistance. LTPs are distributed at high
concentrations in the epidermis of exposed surfaces and in the vascular tissues. In addition to their contribution in host defense mechanisms, LTPs
have been shown to inhibit growth of bacterial pathogens in vitro. Cell wall
extracts from transgenic tobacco plants overexpressing LTP inhibit growth
of the bacterial pathogen Pseudomonas syringae pv. tabaci (Molina and
Garcia-Olmedo, 1997).
Synergistic Action of a Combination of PR Proteins
Toxic action of several individual PR proteins could not be demonstrated. However, when they are combined, they show high toxic action
against fungal pathogens. Either chitinases Ch1 or -1,3-glucanase G2 from
pea pods do not inhibit growth of Fusarium solani f. sp. phaseoli even at a
250 g/mL concentration. However, a combination of Ch1 and G2 effectively inhibits growth of the fungus even at 10 g/mL (Mauch et al., 1988).
Tobacco class I chitinase acts synergistically with tobacco class I PR-4 protein inhibiting Fusarium solani germlings. Tobacco class V chitinases act
synergistically with class I -1,3-glucanase inhibiting growth of Alternaria
radicina (Ohl et al., 1994). A combination of chitinase and ribosome-inactivating protein from barley inhibits fungal growth more efficiently than does
either enzyme alone (Leah et al., 1991). Not one, but several PR proteins are
induced in resistant interactions due to infection (Vidhyasekaran, 1997).
This suggests that a multitude of PR proteins may act synergistically and
contribute to disease resistance.
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Vidhyasekaran, P. (2002). Bacterial Disease Resistance in Plants: Molecular Biology
and Biotechnological Applications. The Haworth Press, Binghamton, NY.
Waniska, R. D., Venkatesha, R. T., Chandrashekar, A., Krishnaveni, S., Bejosano,
F. P., Jeoung, J., Jayaraj, J., Muthukrishnan, S., and Liang, G. H. (2001).
Antifungal proteins and other mechanisms in the control of sorghum stalk rot
and grain mold. J Agric Food Chem, 49:4732-4742.
35
Phenolics
Phenolics
Phenolics are the important phytoanticipins in plants. They are detected
as soluble forms in cytoplasm and are wall-bound in cell walls. They are inhibitory to pathogens at high concentrations. Their content increases rapidly
in resistant interactions, while such an increase is not usually observed in
susceptible interactions in the early stages of infection. The role of phenolics in disease resistance is described in this chapter.
SOLUBLE PHENOLICS AND DISEASE RESISTANCE
Phenolics are commonly present in cell walls (wall-bound phenolics)
and in the cytoplasm (soluble phenolics). Several kinds of phenolics have
been reported in plants. Monophenols, dihydroxy phenols, trihydroxy phenols, phenolics acids, pterocarpans, isoflavans, isoflavones, isoflavanones,
glucosides of isoflavonoids, furanocoumarins, and anthocyanidins are the
common phenolics in plants. Phenolics are synthesized through the phenylpropanoid pathway. They are normal secondary metabolites in healthy
plants. However, they are also induced when pathogens invade a host, similar to phytoalexins and pathogenesis-related (PR) proteins. The concentrations of phenolics existing in healthy plants may not be inhibitory to pathogens (Vidhyasekaran, 1988, 1997, 2002). However, phenolic content increases
severalfold when plants are infected by pathogens. At increased concentrations, phenolics may be toxic.
Several phenolics are found in every plant, similar to phytoalexins and
PR proteins. For example, more than 20 different phenolic compounds have
been detected in French bean. Not all of them are equally toxic to pathogens; toxicity varies from compound to compound. For example, the bean
isoflavone isoferreirin is inhibitory to Cladosporium cucumerinum at a concentration of 10 g/mL, while another bean isoflavanone, dalbergioidin, is
inhibitory to the pathogen only at concentrations above 75 g/mL (Adesanya
et al., 1986). Coumarin, cinnamic acid, p-coumaric acid, vanillic acid,
ferulic acid, and caffeic acid are highly inhibitory to Phytophthora cinnamomi, while protocatechuic acid, catechin, chlorogenic acid, phloroglu-
cinol, and gallic acid are almost noninhibitory to this pathogen (Cahill and
McComb, 1992).
Sensitivity of pathogens to different phenolics also varies from pathogen
to pathogen. Pyrogallol is inhibitory to Phytophthora cambivora and not to
P. cinnamomi (Casares et al., 1986). Similar differential toxic action has
been reported for phytoalexins and PR proteins. Thus, phenolics are similar
to PR proteins and phytoalexins in several aspects. However, phenolics are
different from the other two in that they are present in plants before infection by pathogens or they are produced after infection solely from preexisting constituents. Phenolics belong to another group of defense chemicals
called phytoanticipins. VanEtten and colleagues (1995) defined phytoanticipins as low-molecular-weight antimicrobial compounds that are present in plants before challenge by microorganisms or are produced after
infection solely from preexisting constituents. In addition to phenolics,
terpenoids, glucosinolates, alkaloids, dienes, saponins, and cyanogenic glucosides belong to the group of phytoanticipins.
The soluble phenolics are toxic to fungal and bacterial pathogens. Phenolics may alter the membrane porosity of fungal cells and inhibit certain enzymes of pathogens or DNA transcription (Vidhyasekaran, 1997). Phenolics
may also inhibit production of toxins and pectic enzymes by pathogens.
Rapid increase in phenolic synthesis due to infection has been correlated
with disease resistance in many host-pathogen interactions. In cotton, younger (five- to six-day-old) seedlings are highly susceptible to Rhizoctonia
solani, while older (12-day-old) seedlings are resistant. The concentration
of phenolic compounds, predominantly catechin, increases more in 12-dayold seedlings than in young seedlings due to infection (Hunter, 1978). Some
phenolics, such as salicylic acid, act as signal molecules and trigger host defense mechanisms.
Pathogens are capable of degrading phenolics to nontoxic compounds.
For example, Ascochyta rabiei, the chickpea pathogen, rapidly degrades the
chickpea phenolic biochanin A into nontoxic chemicals (Kraft and Barz,
1985). An early increase in concentration in phenolic content may be necessary to confer resistance against pathogens. Phenolic content increased
mostly in resistant interactions, and if phenolics accumulated in susceptible
interactions, accumulation was always in much later stages than observed in
resistant interactions. The delayed accumulation is characteristic of susceptible interactions.
The role of phenolics in inducing resistance against pathogens has been
demonstrated by altering the phenolic content of plants. When susceptible
plants are fed with phenolics or precursors of phenolics, the plants show enhanced disease resistance (Vidhyasekaran et al., 1986). Preharvest spray of
avocado fruits with thidiazuron (TDZ) or benzylaminopurine (benzylad-
enine) increased levels of epicatechin and resistance to Colletotrichum

gloeosporioides (Beno-Moualem et al., 2001). When resistant plants are infused with phenylalanine ammonia-lyase (the key enzyme involved in biosynthesis of phenolics) inhibitors, phenolic synthesis is suppressed and the
plants become susceptible to pathogens (Carver, Robbins, et al., 1992).
WALL-BOUND PHENOLICS
AND DISEASE RESISTANCE
The plant cell wall is the first barrier, and penetration of the cell wall appears to be the first requirement for pathogenesis of fungal pathogens. Bacterial pathogens multiply in the apoplast. The apoplast constitutes the cell
wall continuum that surrounds the symplast (the symplast constitutes the
sum total of the living protoplasm of a plant). In the area that separates two
cells is a median substance called the middle lamella. Bacterial pathogens,
which enter through natural openings or wounds, multiply in the apoplast
(intercellular spaces), particularly in the middle lamella. The plant cell wall
is considered to be the extracellular matrix secreted into intercellular spaces,
and hence the cell wall may be the first barrier the bacterial pathogens encounter in the intercellular spaces.
When pathogens try to penetrate, the host cell wall is reinforced by different chemicals in resistant interactions. One of them is deposition of
phenolics. Different phenolic compounds have been detected in plant cell
walls. Flavonoids are the important cell wall-bound phenolic compounds.
Flavonoids are synthesized in the cytoplasm and translocated toward the
paramural areas, where they predominantly accumulate in primary cell
walls and middle lamellae. Accumulation of these flavonoids occurred only
in the resistant cotton variety inoculated with the pathogen Xanthomonas
axonopodis pv. malvacearum, and no such flavonoid accumulation was observed in the susceptible variety (Dai et al., 1996).
Phenolic compounds are fluorescent. Autofluorescence can be observed
in association with attempted penetration of the host cell wall by the primary germ tube and the appressorium of fungal pathogens. Autofluorescence is localized in limited regions of the host cell wall surrounding fungal germ tube contact sites. Phenylalanine ammonia-lyase (a key enzyme in
the biosynthesis of phenolics) inhibitors suppress accumulation of localized
autofluorogens, suggesting accumulation of phenolics (Carver, Zeyen, et al.,
1992). Autofluorescence in cell walls has been shown to be associated only
with resistant interactions in many host-pathogen interactions (Godwin et al.,
1987; Cohen et al., 1989).
The nature of induced cell wall-bound phenolics varies. In tomato, an array of phenolic compounds accumulate in cell walls after inoculation with
Verticillium albo-atrum. In the uninoculated cells, only traces of esterified
phenolics are detected. The major compound accumulated in the infected
cell wall is sinapic acid (Bernards and Ellis, 1991). In potato, the induced
cell wall-bound phenolics have been identified as 4-hydroxybenzoic acid,
4-coumaric acid, ferulic acid, 4-coumaroyltyramine and feruloyl-tyramine
(Clarke, 1982). In cotton, the cell wall-bound phenolics are anthocyanin
compounds (Holton and Cornish, 1995).
REFERENCES
Adesanya, S. A., ONeill, M. J., and Roberts, M. F. (1986). Structure-related
fungitoxicity of isoflavonoids. Physiol Mol Plant Pathol, 29:95-103.
Beno-Moualem, D., Vinokur, Y., and Prusky, D. (2001). Cytokinins increase epicatechin content and fungal decay resistance in avocado fruits. J Plant Growth
Regulation, 20:95-100.
Bernards, M. A. and Ellis, B. E. (1991). Phenylalanine ammonia-lyase from tomato
cell cultures inoculated with Verticillium albo-atrum. Plant Physiol, 97:14941500.
Cahill, D. M. and McComb, J. A. (1992). A comparison of changes in phenylalanine ammonia-lyase activity, lignin and phenolic synthesis in the roots of
Eucalyptus calophylla (field resistant) and E. marginata (susceptible) when infected with Phytophthora cinnamomi. Physiol Mol Plant Pathol, 40:315-332.
Carver, T. L. W., Robbins, M. P., Zeyen, R. J., and Dearne, G. A. (1992). Effects of
PAL-specific inhibition on suppression of activated defence and quantitative
susceptibility of oats to Erysiphe graminis. Physiol Mol Plant Pathol, 41:149163.
Carver, T. L. W., Zeyen, R. J., Robbins, M. P., and Dearne, G. A. (1992). Effects of
the PAL inhibitor, AOPP, on oat, barley and wheat cell responses to appropriate
and inappropriate formae speciales of Erysiphe graminis DC. Physiol Mol Plant
Pathol, 41:397-409.
Casares, A., Melo, E. M. P. F., Ferraz, J. F. P., and Ricardo, C. P. P. (1986). Differences in ability of Phytophthora cambivora and P. cinnamomi to dephenolize
lignin. Trans Brit Mycol Soc, 87:229-235.
Clarke, D. D. (1982). The accumulation of cinnamic acid amides in the cell walls of
potato tissue as an early response to fungal attack. In R. K. S.Wood (Ed.), Active
Defense Mechanisms in Plants. Plenum Press, New York, pp. 321-322.
Cohen, Y., Eyal, H., Hanania, J., and Malik, Z. (1989). Ultrastructure of Pseudoperonospora cubensis in muskmelon genotypes susceptible and resistant to
downy mildew. Physiol Mol Plant Pathol, 34:27-40.
Dai, G. H., Nicole, M., Andary, C., Martinez, C., Bresson, E., Boher, B., Daniel,
J. F., and Geiger, J. P. (1996). Flavonoids accumulate in cell walls, middle
lamellae and callose-rich papillae during an incompatible interaction between
Xanthomonas campestris pv. malvacearum and cotton. Physiol Mol Plant Pathol,
49:285-306.
Godwin, J. R., Mansfield, J. W., and Darby, P. (1987). Microscopical studies of resistance to powdery mildew disease in the hop cultivar Wye target. Plant Pathology, 36:21-32.
Holton, T. A. and Cornish, E. C. (1995). Genetics and biochemistry of anthocyanin
biosynthesis. Plant Cell, 7:1071-1083.
Hunter, R. E. (1978). Effects of catechin in culture and in cotton seedlings on
growth and polygalacturonase activity of Rhizoctonia solani. Phytopathology,
68:1032-1036.
Kraft, B. and Barz, W. (1985). Degradation of the isoflavone biochanin A and its
glucoside conjugates by Ascochyta rabiei. Appl Environ Microbiol, 50:45-48.
VanEtten, H. D., Sandrock, R. W., Wasmann, C. C., Soby, S. D., McCluskey, K.,
and Wang, P. (1995). Detoxification of phytoanticipins and phytoalexins by
phytopathogenic fungi. Can J Bot, 73(Suppl. I):S518-S525.
Dekker, New York.
Vidhyasekaran, P., Borromeo, E. S., and Mew, T. W. (1986). Host-specific toxin
36
Phytoalexins
Phytoalexins
Phytoalexins are antimicrobial compounds synthesized in plants due to
infection. Several phytoalexins have been isolated from various plants and
characterized. Their role in disease resistance is described in this chapter.
DEFINITION
Muller and Borger (1940) defined phytoalexins as plant antibiotics that
are synthesized de novo after the plant tissue is exposed to microbial infection. Paxton (1981) defined phytoalexins as low-molecular-weight antimicrobial compounds that are both synthesized by and accumulated in
plants after exposure to microorganisms. Two important criteria have been
suggested to label a secondary metabolite a phytoalexin: (1) the secondary
metabolite should be produced de novo in response to infection, and (2) the
compound should accumulate to antimicrobial concentrations in the area of
infection (VanEtten et al., 1995).
Phytoalexins differ from pathogenesis-related proteins in that PR proteins are produced by transcription of quiescent host genes, while phytoalexin production in plants requires a biosynthetic pathway involving coordinated action of several host enzymes (Ingham, 1973). Phytoalexins are
induced by fungal, bacterial, and viral pathogens, biotic and abiotic elicitors,
various toxic chemicals, and environmental factors. Some compounds may
be phytoalexins in one organ and constitutive in another organ of the same
plant species. Momilactone A, which is induced in rice leaves as a phytoalexin, occurs constitutively in rice seeds. The same compound may be a
phytoalexin in one plant species and a constitutive compound in another
species. The flavanone sakuranetin is induced in rice leaves but is constitutive in blackcurrant (Ribes nigrum) leaves. The same phytoalexin can be detected in more than one plant species. Medicarpin is the phytoalexin detected in chickpea (Cicer arietinum), alfalfa (Medicago sativa), peanut
(Arachis hypogaea), and broad bean (Vicia faba). Luteolinidin has been detected as a phytoalexin in sugarcane (Saccharum officinarum) and sorghum
(Sorghum bicolor). Spirobrassinin is the phytoalexin detected in Brassica
campestris and Raphanus sativus. Resveratrol has been detected in peanut

and Festuca versuta (for more details, see Vidhyasekaran, 1988, 1997,
2002). It appears that induction of phytoalexins is universal in the plant
kingdom. Several phytoalexins have been detected in each plant species,
and as many as 30 phytoalexins in carnation plant have been reported (Niemann, 1993).
ANTIMICROBIAL ACTION OF PHYTOALEXINS
Phytoalexins are inhibitory to microorganisms, and most are highly fungitoxic. Casbene is inhibitory toward the growth of Aspergillus niger even at
10 g/mL. Phytoalexins inhibit spore germination, germ tube elongation,
and mycelial growth of fungi. They may suppress toxin production by
pathogens. Trichothecene toxin production by the parsnip pathogen Fusarium
sporotrichioides is completely inhibited by the parsnip furanocoumarin
phytoalexins (Desjardins et al., 1989). Several phytoalexins have been
shown to inhibit bacterial pathogens in vitro. The lettuce phytoalexin
lettucenin A was found to be highly inhibitory to P. savastanoi pv. phaseolicola. Clear zones of inhibition of bacterial growth were produced by
0.5 g samples of lettucenin A applied to lawns of P. savastanoi pv. phaseolicola (Bennett et al., 1994).
Although phytoalexins are inhibitory to fungal pathogens, the pathogens
of a particular host are less sensitive or insensitive to the phytoalexins of the
host (VanEtten et al., 2001). Actually, pathogens produce enzymes that detoxify the phytoalexins of their host. For example, Nectria haematococca,
Ascochyta pisi, Rhizoctonia solani, Phoma pinodella, and Mycosphaerella
pinodes are all pathogens of pea, and all of them are known to detoxify the
pea phytoalexin pisatin. The pathogens produce pisatin demethylase which
demethylates pisatin, and the resultant product is much less toxic to the
pathogens. Ascochyta rabiei, a pathogen of chickpea, produces a nicotinamide adenine dinucleotide phosphate hydrogen (NADPH)-dependent reductase and detoxifies the chickpea phytoalexin maackiain. Fusarium solani f.
sp. phaseoli, the pathogen of bean, degrades four phytoalexins of the host,
phaseollin, phaseollidin, phaseollinisoflavan, and kievitone, by producing
monooxygenases. Kievitone hydratase produced by the pathogen detoxifies
kievitone.
In some cases, the produced phytoalexins may be inactivated in susceptible interactions. Rogers and colleagues (1996) showed that while Kings
medium B containing 500 g of camalexin was toxic to P. syringae pv.
maculicola, intracellular fluid extracted from Arabidopsis leaves with camalexin added to a concentration of 500 g/mL was not toxic to P. syringae
pv. maculicola. When P. syringae pv. maculicola cells were grown in intercellular fluid and then transferred to Kings medium B containing camalexin, death rate of cells was indistinguishable from that observed when
cells were grown in Kings medium B, suggesting that intercellular fluid
does not induce resistance to camalexin. Camalexin was not degraded in the
intracellular fluid, which suggests that a component of intracellular fluid sequesters camalexin in a nontoxic form (Rogers et al., 1996).
ROLE OF PHYTOALEXINS
IN DISEASE RESISTANCE
No conclusive evidence is available to prove the role of phytoalexins in
disease resistance. However, much indirect evidence suggests their role
in disease resistance. Plants respond to pathogens producing phytoalexins.
Phytoalexins are induced rapidly and more abundantly in resistant interactions. The differences between resistance and susceptibility may be quantitative rather than qualitative. Susceptible plants may possess the machinery
necessary for induction of phytoalexins, but it may not be activated in sufficient magnitude to restrict the infection. Rapid induction of phytoalexins in
resistant interactions may occur even at the stage of transcription of genes
encoding biosynthetic enzymes. For example, both phenylalanine ammonia-lyase (PAL) and chalcone synthase (CHS) mRNAs accumulated in the
incompatible interaction by three hours postinoculation, while in the compatible interaction, PAL transcripts began to accumulate about 15 hours
postinoculation, and CHS transcripts accumulated very weakly around 18
hours postinoculation (Meier et al., 1993). Phytoalexins were detected in
the incompatible interaction by 24 hours postinoculation, while no phytoalexins accumulated in the compatible interactions during this period (Meier
et al., 1993). In susceptible interactions, suppression of induction of phytoalexins would have been suppressed. When bean plants were inoculated
with the incompatible pathogen P. syringae pv. tabaci, phytoalexins were
induced. Prior infiltration with the compatible pathogen P. savastanoi pv.
phaseolicola suppressed the accumulation of phytoalexins induced by the
incompatible pathogen (Jakobek et al., 1993). Accumulation of phytoalexins specifically in resistant interactions suggests a role for phytoalexins
in disease resistance.
The importance of the phytoalexins in disease resistance has been demonstrated by assessing growth of pathogens in host tissues in which phytoalexins start to accumulate during pathogenesis. When lettuce leaves were
inoculated with the incompatible bacterium P. savastanoi pv. phaseolicola,
the phytoalexin lettucenin A accumulated in the inoculated tissues. How-
ever, when these leaves were inoculated with an hrp mutant (hrpD ) of this
bacterium, only trace amounts of the phytoalexin were noticed (Bestwick
et al., 1995). The wild-type bacterium was quickly eliminated (within 48
hours) from the leaf tissues in which phytoalexin accumulated, while the
hrpD mutant of the bacterium survived in the leaf tissues in which no (or
trace amounts of ) phytoalexin accumulated (Bestwick et al., 1995). The
levels of phytoalexin (glyceollin) accumulation in various cultivars of soybean after infection with P. syringae pv. glycinea were inversely correlated
with bacterial multiplication (Long et al., 1985).
The accumulation of stilbene phytoalexin decreased in grape berries during ripening, and these ripening berries became susceptible to Botrytis
cinerea infection (Bais et al., 2000). Application of a plant defense activator, acibenzolar-S-methyl induced systemic resistance against Colletotrichum
destructivum in cowpea, and this treatment resulted in induction of phytoalexins (Latunde-Dada and Lucas, 2001). Mohr and Cahill (2001) provided
evidence that phytoalexins may be more important than phenolic and lignin deposition in disease resistance. In compatible interactions of soybean with Phytophthora sojae, leaves and hypocotyls were characterized by hypersensitive
reaction (HR), phenolic and lignin deposition, and phytoalexin glyceollin
accumulation. Norflurazon treatment restricted the spread of P. sojae and
increased glyceollin accumulation in compatible tissues. Exogenous abscisic
acid addition caused spreading lesions in normally incompatible interactions and reduced glyceollin accumulation. Phenolic deposition and HR
were unchanged by either treatment in the incompatible and compatible interactions. This evidence suggests that glyceollin is a major factor in conferring resistance against the pathogen (Mohr and Cahill, 2001). All these
studies suggest phytoalexins may have a role in disease resistance.
REFERENCES
Bais, A. J., Murphy, P. J., and Dry, I. B. (2000). The molecular regulation of stilbene
phytoalexins biosynthesis in Vitis vinifera during grape berry development. Australian J Plant Physiol, 27:425-433.
Bennett, M. H., Gallagher, M. D. S., Bestwick, C. S., Rossiter, J. T., and Mansfield,
J. W. (1994). The phytoalexin response to lettuce to challenge by Botrytis
cinerea, Bremia lactucae and Pseudomonas syringae pv. phaseolicola. Physiol
108:503-516.
Desjardins, A. E., Spencer, G. F., Plattner, R. D., and Beremand, M. N. (1989).

Furanocoumarin phytoalexins, trichothecene toxins, and infection of Pastinaca
sativa by Fusarium sporotrichioides. Phytopathology, 79:170-175.
Ingham, J. L. (1973). Disease resistance in higher plants: The concept of preinfectional and post-infectional resistance. Phytopathol Z, 78:314-335.
Jakobek, J. L., Smith, J. A., and Lindgren, P. B. (1993). Suppression of bean defense
responses by Pseudomonas syringae. Plant Cell, 5:57-63.
Latunde-Dada, A. O. and Lucas, J. A. (2001). The plant defence activator acibenzolar-S-methyl primes cowpea (Vigna unguiculata (L.) Walp.) seedlings for
rapid induction of resistance. Physiol Mol Plant Pathol, 58:199-208.
Long, M., Barton-Willis, P., Staskawicz, B. J., Dahlbeck, D., and Keen, N. T.
(1985). Further studies on the relationship between glyceollin accumulation and
the resistance of soybean leaves to Pseudomonas syringae pv. glycinea. Phytopathology, 75:235-239.
Meier, B. M., Shaw, N., and Slusarenko, A. J. (1993). Spatial and temporal accumulation of defense gene transcripts in bean (Phaseolus vulgaris) leaves in relation
to bacteria-induced hypersensitive cell death. Mol Plant-Microbe Interact, 6:
453-466.
Mohr, P. G. and Cahill, D. M. (2001). Relative roles of glyceollin, lignin, and the
hypersensitive response and the influence of ABA in compatible and incompatible interactions of soybeans with Phytophthora sojae. Physiol Mol Plant Pathol,
58:31-41.
Muller, K. O. and Borger, H. (1940). Experimentelle untersuchungen uber die
Phytophthora-resistenz der kartoffel Arb. Biol. Reichsasnstalt. Landw Forstw
Berlin, 23:189-231.
Niemann, G. J. (1993). The anthranilamide phytoalexins of the caryophyllaceae and
related compounds. Phytochemistry, 34:319-328.
Paxton, J. D. (1981). PhytoalexinsA working redefinition. Phytopathol Z, 101:
106-109.
Rogers, E. E., Glazebrook, J., and Ausubel, F. M. (1996). Mode of action of the
Arabidopsis thaliana phytoalexin camalexin and its role in Arabidopsis-pathogen interactions. Mol Plant-Microbe Interact, 9:748-757.
VanEtten, H. D., Sandrock, R. W., Wasmam, C. C., Soby, S. D., Mellusky, K., and
Wang, P. (1995). Detoxification of phytoanticipins and phytoalexins by phytopathogenic fungi. Can J Bot, 73(Suppl.1):S518-S525.
VanEtten, H. D., Temporini, E., and Wasmann, C. (2001). Phytoalexin (and phytoanticipin) tolerance as a virulence trait: Why is it not required by all pathogens?
Dekker, New York.
PATHOGENESIS
37
Physiology
Physiologyof
of Pathogenesis
Pathogenesis
Adhesion of fungal and bacterial cells to the host surface triggers the
pathogenesis process. Several molecules of both the pathogen and the host
are involved in the early recognition process. Various enzymes and toxins are
involved in the disease development. This process is described in this chapter.
ADHESION
Pathogenesis appears to start when fungal spores or bacterial cells land
on the surface of the plant. Adhesion of fungi and bacteria to the leaf surface
seems to be important in pathogenesis. Mutants with adhesion-deficient
macroconidia of Nectria haematococca were found to be avirulent (Jones
and Epstein, 1990). The mutants of Pseudomonas savastanoi pv. phaseolicola with greatly reduced adherence to the leaf surface of bean showed
lower incidence of halo blight disease (Romantschuk et al., 1991). Nonattaching mutants of Agrobacterium tumefaciens are known to be avirulent
(Matthysse et al., 1996). Preformed and induced adhesive materials have
been detected in fungal spores. The preformed adhesive material is a protein, and the induced material is a glycoprotein. The glycoprotein adhesive
material is released from ungerminated Colletotrichum graminicola conidia
at an extremely early time in the infection process (Mercure et al., 1994).
Cutinases and other esterases have been shown to be involved in adhesion of
some fungi (Pascholati et al., 1992). Hydrophobins are relatively small
cysteine-rich proteins, and these hydrophobins are involved in attachment
and development of appressoria of fungi (Vidhyasekaran, 1997; Wosten, 2001).
Fimbriae and pili are the filamentous nonflagellar appendages of many bacterial pathogens which may enable the bacteria to attach to plant cells. The
bulk of the filament of fimbriae is made up of helically arranged subunits of
a major protein ranging in size from 9 to 22 kDa in different fimbrial types.
These proteins may be responsible for adhesion of bacterial cells to plant
cells (Vidhyasekaran, 2002). Various strains of P. syringae and P. savastanoi adsorb to leaves of the host plants using their pili (Romantschuk
et al., 1991). Some extracellular polysaccharides of bacteria have been implicated in adhesion. Mutants of Agrobacterium tumefaciens that are defective in neutral glucan production or export are also defective in attachment
to plant cells, suggesting that extracellular neutral glucan is required for attachment and pathogenesis (Thomashow et al., 1987). Lipopolysaccharides
have been implicated in the initial attachment of A. tumefaciens to the plant
cell surface. Rhicadhesin produced by A. tumefaciens is also involved in
adhesion, and some plant molecules may also be involved in adhesion.
Vitronectin-like proteins have been reported in plants, and the binding of A.
tumefaciens by vitronectin has been reported (Wagner and Matthysse,
1992).
NUTRITION
Fungal and bacterial pathogens obtain their nutrition from host cells.
They depend on the host for the synthesis of various constituents of their
cells. Fungal and bacterial cells consist of chitin, cellulose, glucose, mannans,
uronic acids, rhamnose, xylose, proteins, lignin, lipids, nucleic acids, and
pigments. The cell constituents are made up of carbon, nitrogen, phosphorous, sulfur, potassium, magnesium, zinc, iron, manganese, copper, molybdenum, calcium, scandium, vanadium, and gallium. In short, the fungal and
bacterial cells are made up of carbohydrates, fats, proteins, nucleic acids,
and minerals. Host plants supply carbon, nitrogen, and minerals from which
the fungi and bacteria themselves synthesize all the complex cell structures
for their growth.
Bacterial pathogens multiply in the apoplast and obtain nutrients from
the plant cell by causing leakage in cell membranes. A group of bacterial
genes are involved in inducing electrolyte leakage. These include hrp (hypersensitive response and pathogenicity) and avr (avirulence) genes. hrp
genes are required for the elicitation of hypersensitive necrosis on nonhost
plants and on resistant cultivars of the susceptible host, and are also required
for pathogenicity on susceptible cultivars. In both compatible and incompatible interactions, electrolyte leakage is observed. In incompatible interactions, increase in electrolyte leakage may be more during the first few
hours (10 to 30 hours), but subsequent increase in electrolyte leakage is almost similar to compatible interaction (Brisset and Paulin, 1991). hrp genes
are present in all bacterial pathogens, irrespective of whether they are virulent or avirulent. hrp genes appear to be necessary for growth of bacteria in
planta. Bacterial pathogens are able to grow in both host and nonhost plants,
although multiplication may be less in nonhost plants (Vidhyasekaran,
2002). Saprophytic bacteria, which do not possess hrp genes, are unable to
multiply in planta. When saprophytic bacteria carry hrp genes, they are capable of multiplying in the host (Mulya et al., 1996). The major function of
hrp genes appears to be in inducing electrolyte leakage to provide nutrients
to pathogens.
Several hrp genes have been detected in a single bacterial pathogen. For
example, hrp genes encoding 25 Hrp proteins, such as HrpA, HrpB, HrpC,
HrpD, HrpE, HrpF, HrpG, HrpH, HrpI, HrpJ, HrpJ-3, HrpJ-4, HrpJ-5,
HrpK, HrpL, HrpO, HrpR, HrpS, HrpU, HrpU1, HrpU2, HrpW, HrpX,
HrpY, and HrpZ, have been detected in Pseudomonas syringae pv. syringae
strain 61 (Huang et al., 1995). hrp genes appear to be highly conserved in
several bacterial pathogens. The Ralstonia solanacearum strain GMI 1000
hrp gene cluster showed structural homology with all Xanthomonas isolates
tested, namely X. campestris pv. campestris, X. vesicatoria, X. arboricola
pv. juglandis, X. axonopodis pv. begoniae, and X. translucens pv. graminis
(Boucher et al., 1988). The existence of homology between the hrp clusters
of Erwinia amylovora and P. syringae has been reported (Beer et al., 1991).
Homologues to Hrp H and Hrp I of P. syringae pv. syringae have been identified in the hrp clusters of X. vesicatoria (Fenselau et al., 1992) and
R. solanacearum (Gough et al., 1992).
avr genes are pathogen genes critical in determining whether a bacterial
strain will be virulent or avirulent on a specific host (Wanner et al., 1993).
avr genes also appear to be conserved among several bacterial pathogens.
All avr genes of Xanthomonas spp. sequenced to date are 95 to 98 percent
identical to one another. Conservation of avr genes even among different
genera of bacterial pathogens has been reported. Some avr genes appear to
encode a function required for pathogenicity, and some avr genes have been
shown to be virulence factors involved in promoting parasitism (Gopalan
et al., 1996). hrp genes appear to be needed for transcription of avr genes in
bacterial pathogens.
Some hrp and avr genes encode elicitors. The electrolyte (nutrient) leakage may be induced by both hrp and avr gene-encoded elicitors. Both types
of elicitors are secreted into plant cells by the action of hrp genes. A moderate leakage in the host plant may provide the pathogens with various nutrients originating from the plant cell and permit growth of the invading pathogen.
In their life cycle, fungal pathogens enter into two distinct phases,
biotrophic and necrotrophic. In the biotrophic phase, they invade the host
cells inter- and intracellularly and establish themselves in the living cells. In
the second phase, they kill the cells and obtain nutrition from the dying
cells. This phase is called the necrotrophic phase. In the case of obligate
pathogens, the biotrophic phase may be much longer, while in the case of
facultative saprophytes (normally pathogenic but can also live as a sapro-
phyte), a long biotrophic phase may be followed by a necrotrophic phase.

For facultative parasites (normally saprophytes but can also live as a pathogen), the necrotrophic phase will be much longer with a short biotrophic
phase. However, in all pathogens, both phases may exist either as a short or
long spell (Vidhyasekaran, 1993).
PLANT BARRIERS AGAINST PATHOGENS
In the biotrophic phase, pathogens establish themselves in host cells, but
there are many barriers against their establishment. The first barrier between the host plant and the attacking pathogen is the cuticle, which consists of a structural polymer, cutin, impregnated with wax. Suberin is another protective barrier on the surface of plants, and the host cell wall is
another important barrier against the pathogen. Cell walls consist of pectic
substances, hemicelluloses, cellulose, and hydroxyproline rich-glycoproteins. Below the cell wall, membranes are found. The membrane provides a
semipermeable barrier between the cell and its external environment. The
membrane consists of lipids and proteins. During the biotrophic phase, the
pathogens invade the cells by degrading the cuticle, cell wall, and cell membranes by producing a series of enzymes. After the biotrophic phase, the
pathogens produce toxins, which kill the cell and enter into the necrotrophic
phase. Thus, enzymes and toxins are important tools of pathogens for their
pathogenesis.
ENZYMES
Pathogens produce various enzymes to breach the plant barriers to obtain
nutrients from plant cells. Enzymes are complex proteins that catalyze reactions. The following are the important enzymes involved in pathogenesis.
Cutinases
Several fungal pathogens produce cutinases, which degrade the cuticle
layer of the host. Cutinase is considered to be a serine esterase. Variation in
cutinases produced by different pathogens has been observed, as well as
variation in cutinase produced by a single fungal species. Cutinases produced by various fungal pathogens vary remarkably in immunological
properties and primary sequences. Fungal cutinase is not a constitutive enzyme, but it is inducible. Cutinase is released immediately upon contact of
the fungal conidia with a host substratum. Much evidence has demonstrated
the importance of cutinases in pathogenesis. Koller and colleagues (1991)

used a cutinase inhibitor, O-methyl-O-butyl-O-(3,5,6-trichloro-2-pyridyl)
phosphate to assess the importance of cutinase in penetration. When the infection droplets containing spores of Venturia inaequalis and the inhibitor
were incubated on apple leaves, formation of stromata beneath the cuticle
was completely prevented. In samples without the inhibitor, subcuticular
stromata were clearly visible after 24 hours of germination. Virulence of
pathogens has been found to be related to their ability to produce cutinases.
Virulence of the isolates of Fusarium solani f. sp. pisi, the pea pathogen,
was related to their ability to produce cutinase (Koller et al., 1982). Cutinasedeficient mutants of various pathogens show reduced pathogenicity. Introduction of the cutinase gene into a cutinase-less mutant of F. solani restored
pathogenicity (Kolattukudy et al., 1989). When the conidia of avirulent isolate T-30 of F. solani f. sp. pisi were supplemented with cutinase isolated
from a virulent isolate of F. solani prior to inoculation of intact pea-stem
surfaces, the conidia caused severe infection (Koller et al., 1982). All these
studies have shown the importance of cutinases in fungal pathogenesis.
Bacterial pathogens do not penetrate the host cuticle layer; they enter
through wounds and natural openings to cause infection. Hence, there is no
need for bacterial pathogens to produce cutinases.
Pectic Enzymes
After penetration through the cuticle, the invading fungus would encounter the pectinaceous barrier. Even when fungi and bacteria gain entry into
plants through stomata and wounds, they would have to degrade the intercellular pectinaceous polymers to initiate infection in the plant. Plant pathogens produce multiple forms of different types of pectic enzymes. The pectic substances are of two types, pectin (pectinic acid) and pectic acid
(polygalacturonic acid). The activity of the pectic enzymes varies in their
specificity to the substrate (pectin or pectic acid), in their mechanism to split
the -1,4-glycosidic bond (hydrolytic or lyitic), and in the type of cleavage
(random or terminal). The pectic enzymes are classified as pectin methylesterase (PME), which converts pectin into polygalacaturonic acid, exopolygalacturonase (exo PG), which cleaves polygalacturonic acids in a terminal
manner releasing monomeric products (galacturonic acids), and endopolygalacturonase (endo PG), which cleaves polygalacturonic acids in a random
manner releasing oligogalacturonic acid. Exo- and endo-pectin methyl
galacturonases (PMG) act similar to polygalacturonases, but the perferred
substrate is pectin instead of polygalacturonic acid. Both polygalacturonases and polymethylgalacturonases act by hydrolytic action. In contrast,
polygalacturonate trans-eliminase (PGTE; pectate lyase [PL]) and pectin

trans-eliminase (PTE; pectin lyase) induce lyitic degradation of the glycosidic linkage, resulting in an unsaturated bond between carbons 4 and 5 of a
uronide moiety in the reaction product. The trans-eliminases break the
glycoside linkage at carbon number 4 and simultaneously eliminate the H
from carbon number 4, resulting in oligouronides that contain an unsaturated galacturonyl unit. Both exo- (terminal) and endo- (random) type reactions are seen in the trans-eliminases, and they are called exo-PGTE/exo
PL, endo-PGTE /endo PL, exo-PTE/exo pectinlyase, and endo-PTE/endo
pectinlyase (Vidhyasekaran, 1993).
Both fungal and bacterial pathogens produce various pectic enzymes in
vitro and in vivo, and many of them have been purified and characterized
(Vidhyasekaran, 1997, 2002). Histological evidence has been provided to
show the importance of pectic enzymes in cell wall penetration by fungal
pathogens. Penetration of epidermal cells was observed two to seven hours
after inoculation with Pythium ultimum in cucumber (Cherif et al., 1991).
The transmission electron micrographs revealed the alteration of pectic materials in the middle lamella matrices. The pectin breakdown was not restricted to areas neighboring invading hyphae but also occurred at some distance from the point of fungal attack, suggesting that pectin-degradation
enzymes produced by P. ultimum freely diffuse extracellularly, thus facilitating pathogen ingress through loosened host walls (Cherif et al., 1991).
The importance of pectic enzymes in pathogenesis has been demonstrated
using antibodies developed against the enzymes. Fusarium solani f. sp. pisi,
the pea pathogen, produces pectate lyase. Antibodies against the enzyme
were developed. When suspension of the conidia of the fungus was prepared in these antibodies and inoculated on pea stem, no infection of the
pathogen was observed. Penetration of the fungus into the host was strongly
suppressed by the antibodies (Crawford and Kolattukudy, 1987). Fungal
isolates deficient in pectic enzymes have been shown to be less virulent.
Bacterial pathogens produce colonies within intercellular spaces or xylem vessels. These pathogens in the intercellular spaces and xylem vessels
may face the complex pectic polysaccharides, and unless the pectic polysaccharides are degraded by pectic enzymes, they may not be able to breach the
cell well barrier. Bacterial pathogens produce pectic enzymes to breach the pectic barrier. Pectin degradation has been demonstrated during bacterial pathogenesis. Clavibacter michiganensis, the tomato pathogen, quickly spreads
along the xylem vessels of the host. Swelling, shredding, and partial wall
dissolution were typical features in areas adjacent to sites of bacterial accumulation (Benhamou, 1991). Pectin-like molecules and galactose residues
were the major components of the fibrillar or amorphous material accumulating at those sites where bacteria were actively growing (Benhamou,
1991). Because the bacterial pathogens must breach the complex pectic barrier, they need to produce different types of pectic enzymes with varying
specificity to the different types of pectic substances. Production of various
pectic enzymes by bacterial pathogens belonging to various genera, including Pseudomonas, Xanthomonas, Erwinia, Ralstonia, Burkholderia, Agrobacterium, and Clavibacter, has been reported (Vidhyasekaran, 2002).
Cellulases
Cellulose is one of the major components of the plant cell wall. Although
pectin is the main constituent of the middle lamella, cellulose is the main
constituent of xylem tissue. Cellulose is composed of glucose units in the
chain configuration, connected by -1,4-glycosidic bonds. Cellulase is a
general term for a synergistic system of three major categories of enzymes
that hydrolyze the -1,4-glucosidic bonds: endoglucanase (endo -1,4glucanase), exoglucanase ( -1,4-cellobiohydrolase), and -glucosidase. These
are classified according to their mode of action and substrate specificity.
Endoglucanase cleaves -glucosidic bonds in an amorphous region of cellulose, creating sites for exoglucanase to cleave cellobiose from the nonreducing ends. Glucosidase would hydrolyze the resultant cellobiose to glucose, preventing cellobiose buildup and exoglucanase inhibition.
Several cellulolytic enzymes are known to be produced by fungal pathogens. Venturia inaequalis, the apple scab pathogen, produces in culture 12
cellulase isozymes with isoelectric points in the range of 3.7 to 5.6 (Kollar,
1994). These enzymes could also be isolated from apple leaves infected
with the pathogen (Kollar, 1994). Gaeumannomyces graminis var. tritici,
the causal agent of take-all disease of wheat, secretes two groups of enzymes which degrade cellulosic polymers (Dori et al., 1995). The first
group contains an endoglucanase and a -glucosidase with acidic pIs of 4.0
and 5.6, respectively. The second group contains an endoglucanase and a
-glucosidase with basic pIs of 9.3 and >10, respectively. Acidic and basic
groups of endoglucanase and -glucosidase were also obtained from inoculated wheat roots and appeared to be similar to those isolated from the culture fluid (Dori et al., 1995). Several studies have provided evidence to show
the involvement of cellulolytic enzymes in fungal pathogenesis. Histological studies using tobacco roots infected by Phytophthora parasitica var.
nicotianae demonstrated the involvement of cellulases in fungal pathogenesis (Benhamou and Cote, 1992). Incubation of the infected tissues with
the gold-complexed exoglucanase resulted in the deposition of gold particles over both fungal and plant cell walls. Labeling decreased in the inocu-
lated tissues, indicating degradation of cellulose (Benhamou and Cote,

1992).
Xylem degradation has been documented in many bacterial pathogeninfected tissues. Several bacterial pathogens are known to produce cellulolytic enzymes. Clavibacter michiganensis ssp. michiganensis produces an
endoglucanase (Meletzus et al., 1993). Clavibacter michiganensis ssp.
sepedonicus produces exoglucanase, endoglucanase, and -glucosidase (Baer
and Gudmestad, 1995).
Hemicellulases
Hemicelluloses are covalently linked to pectic polysaccharides and noncovalently bound to cellulose fibrils of cell walls. The important plant cell
wall hemicelluloses are xylan, araban, and galactan. Xylans are the most
abundant hemicelluloses, constituting up to 40 percent of the primary cell
walls of some plants. Many fungal pathogens, such as Sclerotium rolfsii,
Verticillium albo-atrum, Fusarium oxysporum f. sp. lycopersici, and Sclerotinia sclerotiorum produce high levels of arabanases, xylanases, and galactanases in vitro. Phytophthora infestans, the pathogen of potato, produces
endo-galactanases in vitro and in infected tissues and depletes galactan
(Jarvis et al., 1981). Arabinose is a major component of the arabinans. It is
also found as a side chain constituent of the xyloglucan, xylan rhamnogalacturonan I, and rhamnogalacturonan II polymers of plant cell walls. The
removal of the arabinose-containing side chains from these major plant cell
wall polymers by arabinofuranosidase might facilitate the digestion of these
polymers. Sclerotinia trifoliorum, a pathogen of legumes, produces an
arabinofuranosidase in culture (Rehnstrom et al., 1994). Three arabinofuranosidase-deficient mutants were obtained. The virulence of the arabinofuranosidase-deficient mutants on alfalfa stems was less than the wild isolates
(Rehnstrom et al., 1994). The bacterial pathogen Clavibacter michiganensis
ssp. michiganensis produces xylanase to degrade xylans (Beimen et al.,
1992).
Proteases
Plant cell walls contain up to 10 percent protein which is exceptionally
rich in hydroxyproline. Fungal pathogens are known to produce various
proteases (Movahedi and Heale, 1990). Several bacterial pathogens produce
proteases. Xanthomonas campestris pv. campestris, X. campestris pv. armoraciae, P. syringae pv. tomato, E. carotovora, and Erwinia chrysanthemi
produce proteases. The pathogens may produce multiple proteases. Erwinia
carotovora ssp. carotovora isolate 177 produces a metalloprotease which

degrades lectin (Heilbronn et al., 1995).
TOXINS
Toxins play an important role in the necrotrophic phase of pathogenesis.
Toxins are defined as microbial metabolites, which are harmful to plants at
physiologically low concentrations. These toxins induce characteristic symptoms of diseases. Several types of toxins have been detected in pathogens.
Host-specific toxin is a metabolic product of a pathogenic microorganism
that is toxic to only the host of that pathogen when used at physiological
concentrations. At high concentrations, even nonsusceptible plants are affected. Some authors prefer the word host-selective toxin for hostspecific toxin, because this toxin cannot be strictly called specific, as at
high concentrations it induces symptom development in resistant plants as
well. Pathotoxin is the toxin that is the major determinant of disease and
pathogenicity. Pathotoxin induces all the typical disease symptoms at physiological concentrations and is correlated with pathogenicity. Host-specific
toxins are considered to be pathotoxins. The toxin produced in vivo (in the
infected tissues), which functions in disease development but not as a primary agent, is called vivotoxin. Nonspecific toxin is the toxin that is toxic to
both hosts and nonhosts. Toxin produced by pathogens, which is toxic to plants
but not considered to be of primary importance during pathogenesis, is
called phytotoxin. Nonspecific toxins are considered to be phytotoxins.
The toxin required for pathogenicity is called the primary determinant. It
is essential for colonization by the producing pathogen and for disease development in the host. The primary determinant is otherwise called the
pathogenicity factor. The toxin required for virulence is called the secondary determinant. The secondary determinant accounts for certain symptoms
or contributes to virulence but is not essential for colonization. The secondary determinant is otherwise called the virulence factor. Pathogenicity refers to the capacity of an organism to induce disease. Virulence is a quantitative term denoting the relative disease-inducing ability of a microorganism.
A change in host range indicates a change in pathogenicity, while a change
in severity of disease indicates a change in virulence. Toxin is not required
for penetration of fungal pathogens into a host. Both toxin-producer and
nonproducer pathogens penetrate host tissues (Vidhyasekaran et al., 1986).
Initial colonization of both types of isolates is also the same in many
hostpathogen interactions. Only subsequent invasion is different. It is most
likely that toxins play an important role only as a virulence factor rather than
as a pathogenicity factor. In other words, toxin is important in the necrotrophic phase rather than in the biotrophic phase (Vidhyasekaran, 1993).
Several types of toxins have been detected in fungal and bacterial pathogens. Some pathogens produce host-specific toxins, while many others produce nonspecific toxins. Some important host-specific toxins characterized
from pathogens are HV-toxin (or victorin) produced by Helminthosporium
victoriae, the oat blight pathogen; HM-T toxin produced by H. maydis race
T, the maize leaf blight pathogen; HS-toxin produced by H. sacchari, the
sugarcane pathogen; HC-toxin produced by H. carbonum, the maize leaf
spot pathogen; HO-toxin produced by H. oryzae, the rice brown spot pathogen; AAL-toxins (AAL toxins TA and TB; Mesbah et al., 2000) produced
by Alternaria alternata f. sp. lycopersici, the tomato pathogen; AF-toxins I,
II, and III produced by A. alternata strawberry pathotype; AK-toxins (three
related toxins) produced by A. kikuchiana, the Japanese pear black spot
pathogen; AL-toxins I and II produced by A. alternata tomato pathotype;
AM-toxins I, II, and III produced by A. alternata apple pathotype; ACRtoxin produced by A. alternata rough lemon pathotype; ACT-toxin produced
by A. alternata tangerine pathotype; AT-toxin produced by A. alternata tobacco pathotype; PC toxin produced by Periconia circinata, the sorghum
pathogen; and PM-toxin produced by Phyllosticta maydis, the maize pathogen. Stemphylium vesicarium, the cause of brown spot of European pear
plants, produces two host-specific SV-toxins (Singh et al., 2000). Rhizoctonia solani, the rice sheath blight pathogen produces a host-specific toxin,
RS-toxin (Vidhyasekaran et al., 1997).
Several other pathogens produce toxins that are involved in symptom development, and most pathogens produce several toxins. Pyricularia oryzae,
the rice blast pathogen, produces piricularin, picolinic acid, pyriculol, and
tenuazonic acid. Helminthosporium oryzae, the rice pathogen, produces
ophiobolin A, ophiobolin B, 6-epiophiobolin A, anhydroophiobolin A,
6-epianhydroophiobolin A, and ophiobolin 1. Alternaria helianthi, the sunflower pathogen, produces radicinin, radianthin, deoxyradicinol, and 3epideoxyradicinol. Alternaria carthami produces brefeldin A, zinniol, and
dehydrobrefeldin A. As a single pathogen produces several toxins, a single
toxin is produced by several pathogens. Ophiobolin A is produced by the
rice pathogen H. oryzae, the maize pathogen H. maydis, and the foxtail millet pathogen H. setariae. Tenuazonic acid is produced by several Alternaria
spp., Pyricularia oryzae, Phoma sorghina, and Aspergillus spp. Cercosporin is produced by Cercospora nicotianae, C. canescens, C. kikuchii,
C. zinniae, C. citrullina, C. ricinella, and C. apii. Polanrazines C and E, the
toxins produced by Phoma lingam (Leptosphaeria maculans), produce necrotic and chlorotic lesions on brown mustard leaves (Pedras and Biesenthal, 2001). Colletotrichin, the toxin produced by a mango leaf isolate of
Colletotrichum gloeosporioides, caused anthracnose-like symptoms on young

mango leaves (Jayasankar et al., 1999). Colletotrichum dematium, the
causal agent of mulberry anthracnose, produces phytotoxins in vitro and in
planta (Yoshida et al., 2000). The potato common scab pathogen Streptomyces scabies produces two phytotoxins, thaxtomin A and thaxtomin B
(King et al., 2000).
Toxins produced by bacterial pathogens cause watersoaking, chlorosis,
and necrosis (Bender et al., 1999). All bacterial toxins studied so far are
nonspecific toxins. All bacterial toxins increase disease severity; in other
words, they act as virulence factorsa quantitative trait. As an exception,
for Pseudomonas syringae pv. tabaci isolate BR2 on bean, tabtoxinine- lactam production is required for disease production (Durbin, 1991). Coronatine is produced by P. savastanoi pv. glycinea, P. syringae pv. maculicola,
P. syringae pv. tomato, P. syringae pv. atropurpurea, and P. syringae pv.
morsprunorum. Coronatine induces chlorotic symptoms, and at higher concentrations, coronatine can cause a necrotic reaction in the central portion of
the spot.
Phaseolotoxin is produced by the bean halo blight pathogen P. syringae
pv. phaseolicola and induces chlorosis. The toxin inhibits ornithine carbamoyltransferase (OCTase), resulting in deficiency of arginine, which
leads to reduction in chlorophyll biosynthesis (Durbin, 1991). Tabtoxinine-lactam is produced by the tobacco wild fire pathogen P. syringae pv.
tabaci, the oat bacterial blight pathogen P. coronafaciens, and some other
P. syringae pathovars. Pseudomonas syringae pv. tabaci produces only the
dipeptide, protoxin form, trivially named tabtoxin, which then is enzymatically hydrolyzed to tabtoxinine- -lactam when it enters the host cell (Uchytil
and Durbin, 1980). Tabtoxin has no biological activity. Tabtoxinine- lactam is the biologically active form of the toxin. Tabtoxinine- -lactam inhibits glutamine synthetase, and this inhibition leads to a rapid buildup of
ammonia. Accumulation of ammonia may cause the initial chlorosis by rapidly disrupting the chloroplasts internal membrane system (Durbin, 1991).
Syringomycin is produced by many strains of P. syringae pv. syringae,
which cause holcus spot of maize, bacterial canker of stone fruits, and
brown spot of bean. The toxin affects ion transport in the plasma membrane.
Pseudomonas syringae pv. syringae produces another toxin, syringopeptin
(Scholz-Schroeder et al., 2001). Tagetitoxin is produced by P. syringae pv.
tagetis and produces chlorotic and necrotic spots. Extracellular polysaccharides (EPS) are produced by several bacterial pathogens (Vidhyasekaran
et al., 1989; Sridhar et al., 2001). Da Silva and colleagues (2001) showed the
involvement of an EPS in pathogenicity of Xylella fastidiosa. This EPS consists of polymerized tetrasaccharide repeating units assembled by the sequential addition of glucose-1-phosphate, glucose, mannose, and glucur-
onic acid on a polyprenol phosphate carrier. Wilting may be induced by the

EPS in several ways. Polysaccharides may block the vascular elements at
the basal end of the stem or may pass through large vessels of the stem and
petiole and accumulate in tiny veinlets and ultrafilters of the pit membrane.
EPS transported to leaves may disrupt water transfer from veins and accumulate in membranes of parenchymatous cells and block movement of solutions through cell membranes. Polysaccharides may also accumulate on primary cell walls in the leaf mesophyll, interfering with water movement
from veins to the evaporative surfaces. Increase in cell membrane permeability may be induced by EPS, which would result in water imbalance and
wilting (Vidhyasekaran et al., 1989).
The importance of toxins in pathogenesis has been demonstrated by several methods. When the toxin-producing ability of a pathogen is suppressed,
the pathogen loses its virulence. CFP (cercosporin facilitator protein), a
gene from the soybean fungal pathogen Cercospora kikuchii, encodes the
putative major facilitator transporter of the fungal toxin cercosporin. Gene
disruption of CFP in C. kikuchii resulted in dramatically reduced cercosporin
production, and this led to reduced virulence of the pathogen (Upchurch
et al., 2001). Disruption of the AM-toxin synthetase gene (AMT) by transformation of wild-type Alternaria alternata apple pathotype with disruption vectors resulted in toxin-minus mutants, and these mutants were unable
to cause disease symptoms on susceptible apple cultivars (Johnson et al.,
2000). Disruption of the sypA gene (encoding a peptide synthetase) in
P. syringae pv. syringae strain B301D resulted in the loss of syringopeptin
production (Scholz-Schroeder et al., 2001). Another gene, sypB1, is involved in syringomycin toxin production by P. syringae pv. syringae.
Syringopeptin (sypA) and syringomycin (syrB1) mutants were reduced in
virulence by 59 and 26 percent, respectively, compared to the parental strain
in cherry, whereas the syringopeptin-syringomycin double mutant was reduced by 76 percent in virulence. This suggests that syringopeptin and
syringomycin are major virulence determinants of P. syringae pv. syringae
(Scholz-Schroeder et al., 2001). Three virulence-deficient mutants of Xanthomonas oryzae pv. oryzae, the causal agent of bacterial blight of rice, were
isolated by Tn5 mutagenesis (Sridhar et al., 2001). These mutants also became deficient in EPS production. A 40 kb cosmid clone that restored EPS
production restored virulence to all three mutants (Sridhar et al., 2001).
ROLE OF TOXINS IN PATHOGENESIS
The major role of toxins appears to be suppression of the defense mechanisms of the host. When a highly virulent isolate of Helminthosporium
oryzae (Cochliobolus miyabeanus), the rice brown spot pathogen, was inoculated on rice leaves, the spores germinated, formed appressoria, and penetrated the epidermal layers at 12 hours after inoculation (Vidhyasekaran
et al., 1986). Extensive inter- and intracellular mycelial growth was observed in mesophyll tissue of leaves at 48 hours after inoculation. When a
nonpathogenic isolate of H. oryzae was inoculated on rice leaves, the isolate
did not grow beyond the infection peg. The pathogenic isolate induced typical brown spot symptoms with yellow halo at 48 hours, while the nonpathogenic isolate did not produce any visible symptoms. When spore suspensions of the nonpathogenic isolate were prepared in H. oryzae toxin and
inoculated on rice leaves, extensive inter- and intracellular mycelial growth
was observed at 36 hours. The growth rate of the mycelium of the nonpathogenic isolate was similar to that of the virulent isolate. Typical brown spot
symptoms appeared on leaves exposed to the toxin (Vidhyasekaran et al.,
1986).
Alternaria alternata is a nonpathogen of rice. Helminthosporium oryzae,
the brown spot pathogen of rice, produces ophiobolin A. When spores of
A. alternata were suspended in ophiobolin A solution, the spores germinated well and formed appressoria and infection pegs on the rice leaf sheath
surface. Extensive inter- and intracellular hyphal growth was observed only
in the sheath tissues that had been inoculated in the presence of ophiobolin
A. The spores without ophiobolin A could not grow beyond the infection
peg and did not grow inside the cell (Xiao et al., 1991). Ophiobolin A at
3 g/mL induced susceptibility of rice leaf tissues to the nonpathogen A.
alternata (Xiao et al., 1991). The toxin inactivated calmodulin (Au et al.,
2000). The role of calmodulin in host defense mechanism is well known
(Vidhyasekaran, 1997, 2002). The toxin produced by Pyricularia oryzae induced susceptibility of rice leaves to the nonpathogen A. alternata (Fujita et
al., 1994).
Helminthosporium oryzae produces a host-specific toxin. When an avirulent isolate of H. oryzae was inoculated along with the toxin, the avirulent
isolate could penetrate the rice leaves and grow profusely. Typical brown
spot lesions also developed (Vidhyasekaran et al., 1986). HV-toxin, produced by Helminthosporium victoriae reduces the accumulation of avenalumins, the phytoalexins, in oats (Mayama et al., 1986). Papilla formation
at the penetration sites of A. kikuchiana functions as a resistance mechanism
in Japanese pear. When pear leaves were inoculated with avirulent spores
plus AK-toxin, the rate of papilla formation reduced and the avirulent
spores could invade susceptible leaves (Otani et al., 1995). Thus, the pathogens appear to suppress the defense mechanisms of the host by producing
toxins. It appears that detoxification alone may allow the resistance genes to
function in plants. The resistance gene cloned from maize has been shown
to be involved in detoxification of the toxin produced by H. maydis (Meeley

and Walton, 1991; Johal and Briggs, 1992).
Toxins may induce leakage of nutrients from the plant cell and make
them available to pathogens in the infection court. The plasma membrane in
the plant cells governs all permeability processes from simple diffusion to
active transport. Modification of the plasma membrane in infected tissues
may result in an efflux of nutrients to the pathogen. Changes in the membrane are mostly induced by toxins produced by pathogens. The toxins induce electrolyte leakage from cells (Vidhyasekaran et al., 1986). Cercosporin, the toxin provided by Cercospora species, generates active oxygen
species and destroys the membranes of host plants, providing nutrients to
support the growth of pathogens (Daub and Ehrenshaft, 2000).
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Glossary
Glossary
This glossary was prepared after consulting several papers and the following books: The Commonwealth Mycological Institute (1974), Plant Pathologists Pocketbook, Kew, Surrey, Great Britain; Parry, D. W. (1990),
Plant Pathology in Agriculture, Cambridge University Press, Cambridge,
Great Britain; Vidhyasekaran, P. (1993), Principles of Plant Pathology,
CBS Publishers, Delhi, India; Hawksworth, D. L., Kirk, P. M., Sutton, B. C.,
and Pegler, D. N. (1995), Ainsworth and Bisbys Dictionary of the Fungi,
CAB International, Dew, Surrey, Great Britain; Nicholl, D. S. T. (1994), An
Introduction to Genetic Engineering, Cambridge University Press, Cambridge, Great Britain.
acervulus: Typically a flat, open bed of stromatic mass of hyphae giving
rise to generally short conidiophores. Conidia are borne at the tips of the conidiophores.
adjuvants: Materials added to improve some chemical or physical property
of a plant protectant.
agglutinin: An antibody that causes a particulate antigen to clump and settle out of suspension.
allele: Chromosomes are usually arranged as homologous pairs, and different forms of the same genes are called alleles.
alternate host: One of two hosts required by a pathogen to complete its life
cycle.
alternative host: One of several plant species hosts of a given pathogen.
amphitrichous: Having one flagellum at each pole.
analytic models: Used to analyze epidemics on a theoretical basis without
taking into consideration the effects of external variables.
anamorph: Some fungi have two valid names: The perfect state (see PERFECT STATE), i.e., the teleomorph, and an imperfect state (see IMPERFECT
STATE),
i.e., the anamorph. The former takes precedence for the name of a
whole fungus or holomorph.
antheridium: The male gametangium of fungi.
antibiotic: A chemical substance produced by a microorganism that is able
to inhibit growth of other microorganisms.
antibody: An immunoglobulin that specifically recognizes and binds to an
antigenic determinant of an antigen. Antigens (see ANTIGEN) stimulate the
immune system of the animal and this leads to the production of specific antibodies, each of which recognizes and binds to its complementary antigen.
Antibodies can recognize the plant pathogen by recognizing the antigen
specific to the pathogen.
anticodon: The three bases on a tRNA molecule that are complementary to
the codon on the mRNA.
antigen: A molecule that is bound by an antibody and can induce an immune response. Cells of living animals, particularly mammals, have the
ability to recognize binding sites on proteins, glycoproteins, lipopolysaccharides, and carbohydrate molecules that are not present in their bodies
(foreign to that animal). Such molecules are known as antigens.
antiserum: Serum that contains antibodies.
apoplast: The total nonliving cell wall continuum that surrounds the symplast (see SYMPLAST). Xylem system constitutes a specialized phase of the
apoplast. It also constitutes a continuous permeable system through which
water and solutes may move freely.
apothecium: An open ascocarp in which asci are produced (plural: apothecia).
apparent infection rate: The rate of infection, which is calculated by taking into account the amount of plant tissue left to be colonized.
appressorium: A swollen fungal hyphal tip usually associated with the
mechanism of adherence to the plant surface prior to penetration.
area under disease progress curve (AUDPC): The amount of disease integrated between two times of interest. Calculated without regard to curve
shape, AUDPC provides a valid statistical description of disease progress
data.
ascocarp: A fruiting body in which asci are produced.
ascospore: A sexually produced spore borne in an ascus.
ascostroma: A stromatic ascocarp bearing asci directly in locules within

the stroma.
ascus: A spore sac containing ascospores (plural: asci).
autoecious rust: A rust pathogen that completes its life cycle on one host.
avirulence genes: Genes that are critical in determining whether a pathogenic strain will be virulent or avirulent on a specific host. Avirulence genes
restrict a pathogens host range by specifying the resistance response on
host plants carrying complementary disease resistance genes.
avirulent: Lacking virulence.
bacteria: Primitive organisms classified as prokaryotes, with a primitive
type of nucleus lacking a clearly defined membrane. Most of the genetic information in a bacterial cell is carried on a single chromosome with doublestranded deoxyribonucleic acid (DNA) in a closed circular form.
bactericide: A substance causing death of bacteria.
bacteriophage: A virus infecting bacteria.
basidiocarp: A fruiting body that bears basidia.
basidiospore: A spore borne on the outside of a basidium.
basidium: A specialized spore-producing body bearing a definite number
of basidiospores (plural: basidia).
biotroph: A pathogen that obtains nutrients from the living cells of the host.
cDNA: DNA which is made by copying mRNA using the enzyme reverse
transcriptase.
chlamydospores: The hyphal cells of fungi that become enveloped in a
thick wall before they separate from one another or from other hyphal cells
adjoining them.
chromista: A kingdom which includes organisms that bear flagella with
mastigonemes. Cell walls of these organisms do not contain chitin, but contain cellulose and glucan.
chromosome: A DNA molecule carrying a set of genes.
cis-acting element: A DNA sequence that exerts its effect only when on the
same DNA molecule as the sequence it acts on.
cleistothecium: A completely closed ascocarp is called a cleistothecium
(plural: cleistothecia).
codon: The three bases in mRNA that specify a particular amino acid during translation.
coenocytic: A continuous mass of cytoplasm and nuclei without any septa.
complementary genes: Nonallelic genes that complement one another.
complementation: Appearance of a wild phenotype in an organism or cell
containing two different mutations combined in a hybrid diploid or a
heterokaryon.
complementation test: The introduction of two mutant chromosomes into
the same cell to determine whether the mutations in question occurred in the
same gene. The wild phenotype will be expressed, since each chromosome
makes up for or complements the defect in the other.
complete resistance: Very high resistance that suppresses disease development completely.
conidiophore: A specialized hyphal branch bearing conidia.
conidium: An asexually produced fungal spore (plural: conidia).
copy number: The number of copies of a gene in the genome of an organism.
cosmid: A hybrid plasmid/bacteriophage vector that is made up of plasmid
sequences joined to the cos sites of phage .
cytoplasmic inclusion bodies: Some of the water-soluble reserve food materials manufactured by a cell get dissolved in the cell sap. Insoluble constituents precipitate out as cytoplasmic inclusion bodies. Volutin, glycogen,
and fat globules are common cytoplasmic inclusions.
differential variety: A variety that gives reactions which distinguish between race-specific isolates of a pathogen.
disease: A harmful deviation from normal functioning of plant physiological processes. The plant is considered diseased when symptoms, by which
the disease may be recognized, are present.
disease progress curve: The progress of a disease may be assessed as disease incidence (proportion of diseased plants in a plant population) or as
disease severity (proportion of diseased tissue in a plant). When the results
are plotted against time, the curve obtained is commonly S-shaped.
disease-specific (dsp) genes: Genes involved only in pathogenicity.
This is in contrast to hrp genes that are involved both in pathogenicity and
hypersensitive response (see hrp GENES).
dominant gene: The gene that is phenotypically manifested, for example, it

may suppress the development of disease symptoms. It produces a functional product such as disease resistance. Its recessive allele cannot phenotypically manifest and its action is marked in the presence of a dominant
gene (see RECESSIVE GENE).
dot-blot: A technique in which small spots or dots of nucleic acid are immobilized on a nitrocellulose membrane for hybridization.
dot-blotting: Similar to southern and northern blotting in analysis of nucleic acids, but the samples are not subjected to electrophoresis. The nucleic
acid samples are spotted onto the nitrocellulose membranes or nylon filters
and hybridized with a radioactive probe.
durable resistance: Resistance that remains effective in a cultivar that is
widely grown for a long period of time in an environment favorable to the
disease.
elicitors: Signal molecules of pathogens and plants that trigger defense mechanisms of the host.
empirical models: Epidemiological models that are based on actual experiments and observations rather than theory or simulation. They are complex
models that attempt to mimic many environmental factors and other influences.
emulsifiers: Chemicals that assist the formation of a suspension of small
droplets of a liquid in another liquid in which the first liquid is insoluble.
Emulsifiers help in the formation of an emulsion comprising small spheres
of organic solvent/fungicide in the sprayer.
endemic: A disease established in moderate or severe form in a defined
area. The disease level in an endemic disease remains almost constant.
endopolygalacturonase (endo PG): An enzyme that cleaves polygalacturonic acids in a random manner and releases oligogalacturonic acid.
enhancer: A sequence that enhances transcription from the promoter of a
gene; it may be several thousand base pairs away from the promoter.
epidemic: A widespread temporary increase in the incidence of an infectious disease. A plant disease is described as epidemic when the amount of
disease present increases rapidly from a low level to a high one.
epidemiology: The science that describes the progress of a disease as it becomes epidemic (see EPIDEMIC).
episomes: Autonomous and dispensable genetic elements similar to plasmids

(see PLASMID). Unlike plasmids, however, episomes can exist even when
integrated with the chromosome. Some bacteria contain episomes.
epistasis: Phenomenon in which one gene suppresses the expression of another gene. It is generally observed when two or more resistance genes are
present together. Only the gene conditioning the lowest infection type is expressed. For example, expression of recessive genes is suppressed by the
presence of dominant genes.
exopolygalacturonase (exo PG or Peh): An enzyme that cleaves polygalacturonic acids in a terminal manner and releases monomeric products
(galacturonic acids).
facultative parasite: A parasite that can also live as a saprophyte. Facultative parasites can be cultured on laboratory media.
facultative saprophyte: A saprophyte that can also live as a parasite.
flagellum: A whiplike appendage of a motile cell (plural: flagella).
foam suppressors: Surface-active substances that form a fast draining
foam to provide maximum contact of the fungicide spray to the plant surface.
forma specialis (f. sp.): An intraspecific category for taxa characterized
from a physiological standpoint (particularly based on pathogenicity to different species or varieties of host plants) and may be indistinguishable morphologically (plural: formae speciales, ff. sp.).
fructifications: Structures containing spores in fungi.
fumigation: Disinfestation by fumes.
fungi: Organisms that are eukaryotic and heterotrophic. Fungi develop
branching filaments (these organisms are more rarely single-celled), reproduce by spores, and their cell walls contain chitin and -glucans. They are
mostly nonflagellate. When present, flagella always lack mastigonemes
(i.e., the surfaces of flagella are not covered by hairlike processes).
fungicidal: Able to kill fungal spores or mycelium.
fungicide: A chemical substance that kills fungal spores or mycelium.
fungicide resistance: A decrease in sensitivity to a fungicide due to selection or mutation following exposure to the compound.
fungistatic: Able to stop fungal growth without killing the fungus.
gene: The unit of inheritance, located on a chromosome. It is a sequence of

DNA that codes for a diffusible protein product, which diffuses away from
its site of synthesis to act elsewhere. In molecular terms, the gene means a
region of DNA that encodes one function. Broadly, therefore, one gene encodes one protein.
gene cloning: Generation of recombinant DNA molecules in the laboratory.
gene-for-gene hypothesis: An assumption that corresponding genes for resistance and virulence exist in host and pathogen respectively. According to
this hypothesis, for each gene that conditions resistance in the host, there is a
corresponding gene that conditions pathogenicity (virulence) in the pathogen. No resistance occurs unless a resistance is present in the host along
with a corresponding avirulence gene in the pathogen.
general/partial/field resistance: The disease resistance that provides only
partial or field resistance to several races of a pathogen. This resistance affects the apparent infection rate and the amount of disease that finally develops on the plants.
genetic engineering: A technology that involves cutting, modifying, and
joining DNA molecules using enzymes such as restriction enzymes and
DNA ligase. It is also known as gene cloning, recombinant DNA technology, molecular cloning, or gene manipulation.
germ tube: The initial hyphal growth from a germinating fungal spore.
green bridge: Living plant material used by biotrophs to overwinter.
groundkeeper: A self-sown plant.
haustorium: A specially developed fungal hyphal branch within a living
cell of the host for absorption of food (plural: haustoria).
helper viruses: Viruses that help multiplication of satellite RNA (see SATELLITE VIRUS).
hemibiotroph: A pathogen living at first as a biotroph but later as a necrotroph.
heteroecious: A pathogen requiring two host species to complete its life cycle.
heterothallism: A condition in which sexual reproduction in fungi occurs
between oogonium and antheridium from two different sexually compatible
mycelia (thalli).
holomorph: See ANAMORPH.

homothallism: A condition in which sexual reproduction in some fungi occurs between oogonium and antheridium, even from the same thallus.
horizontal resistance: Resistance against several races of a pathogen.
host-selective toxin: The toxin that is selectively toxic to some hosts. It is
similar to host-specific toxin (see HOST-SPECIFIC TOXIN). Some authors prefer the word host-selective toxin for host-specific toxin, because this
toxin cannot be strictly called host-specific, as at high concentrations it induces symptom development in resistant plants as well.
host-specific toxin: A metabolic product of a pathogenic microorganism
that is toxic only to the host of the pathogen when used at physiological concentrations. At high concentrations, even nonsusceptible plants are affected.
host-specific virulence (hsv) genes: Genes that are required for virulence on specific hosts.
hrp genes: Bacterial genes that are required for growth in planta and
initiation of disease symptoms on host plants and induction of defense
mechanisms as expressed by hypersensitive reaction on nonhost plants. Hypersensitive response and pathogenicity (hrp) genes are general virulence
genes that are required for virulence on all hosts. The hrp genes are not hostspecific; their inactivation leads to loss of virulence on all hosts and loss of
the nonhost hypersensitive response.
hymenium: A fertile layer consisting of asci (whether naked or enclosed in
a fruiting body) or basidia. Sterile, elongated hairs, arising between the asci,
often form a part of the hymenium (plural: hymeia).
hyperparasitism: Parasitism on another parasite.
hyperplasia: Overdevelopment of some tissues due to abnormal multiplication of cells.
hypersensitivity: A rapid local reaction of plant tissue to an attack by a
pathogen, which results in the death of tissue around infection sites and prevents further spread of infection.
hypertrophy: Abnormal enlargement of an organ or part resulting from an
increase in the size of the cells.
hypha: A tubular threadlike filament of fungal mycelium (plural: hyphae).
hyphopodium: A short mycelial branch (plural: hyphopodia).
hypoplasia: Subnormal cell multiplication. It often results in the dwarfing

of plants.
immune: Exempt from infection.
imperfect state: The asexual period of a fungal life cycle.
incubation period: The period of time between infection and the appearance of symptoms.
infection: The penetration of the host by a pathogen and the earliest stages
of development within the host.
infection court: The initial site of contact between infection and the appearance of symptoms.
infection peg: A slender structure formed by the deposition of substances,
such as lignin, around a thin hypha penetrating a host cell.
inhibitins: Antimicrobial substances in plants. Their concentration in uninfected (healthy) plants may normally be low, but may increase enormously
after infection (probably in order to combat attack by microorganisms).
inoculum: Spores or other pathogen parts that can cause disease.
inoculum potential: Amount of inoculum required to cause disease.
intercellular: Between cells.
intracellular: Within or through cells.
karyogamy: A phase in which the fusion of two nuclei occurs.
Kochs postulates: The conditions stipulated by Koch to describe an organism as the cause of a disease. The postulates include the following: the organism must be consistently associated with the symptoms of the disease;
the organism must be isolated and grown in pure culture, free from all other
organisms; the organism from the pure culture must be inoculated onto
healthy plants of the same species from which they were originally isolated
and must reproduce the same disease as was originally observed; the organism must be reisolated and reinoculated and must once again reproduce the
original disease. Kochs postulates cannot be fulfilled in the case of an obligate pathogen: this pathogen cannot be isolated and grown in pure culture;
instead, the pathogen may be multiplied on a healthy susceptible host.
latent period: The time between infection and sporulation of the pathogen
on the host, or the time from the start of a virus vectors feeding period until
the vector is able to transmit the virus to healthy plants.
lodging: Breakage of many plant stems, especially cereals, resulting in

many tillers falling down.
major gene: A gene with pronounced phenotypic effects in contrast to its
modifiers.
major-gene resistance: Resistance is governed by major genes (see MAJOR
GENE). Major genes are those that have large, distinct phenotypic expressions showing clear Mendelian segregation.
mastigonemes: The hairlike processes that cover the surface of flagella.
meiosis: A reduction division that reduces the number of chromosomes to
haploid.
messenger RNA (mRNA): The ribonucleic acid transcribed from DNA
that carries the codons specifying the sequence of amino acids in a protein.
microbial pesticides: Introduced microbial agents for control of pests and
diseases.
middle lamella: A median substance found in the area that separates two
cells.
minor genes: Those genes that have small effects on the expression of the
phenotype for resistance and show quantitative segregation.
minor-gene resistance: The resistance is governed by minor genes (see MINOR GENES).
monocyclic: Having only one cycle of infection during a growing season.
monogenic resistance: Resistance is governed by single gene.
monotrichous: Having one polar flagellum.
multiline: A combination of almost genetically identical breeding lines
(isogenic) that have all agronomic characters in common but differ in a
major-gene resistance.
mycelium: A mass of hyphae that form the vegetative body of a fungus.
mycorrhiza: A symbiotic association of a fungus with the roots of a plant.
mycotoxin: Toxins produced by fungi, which may contaminate foodstuffs.
necrotroph: A pathogen that kills the host cells and lives on the dead remains.
nonspecific toxin: A toxin that is toxic to both hosts and nonhosts.

Northern blotting: A method to detect RNA molecules by electrophoresis.
notifiable disease: A disease that must be reported to the appropriate authorities, as per law.
obligate parasite: An organism that is capable of living only as a parasite.
oogonium: The female gametangium of fungi.
operon: A cluster of genes under the control of a single promoter/regulatory region.
parasexual cycle: Through this mechanism, recombination of hereditary
properties occurs through mitosis in fungi.
parasite: An organism that lives on another living organism (host), obtaining its nutrient supply from the host.
pathogen: An organism that causes disease.
pathogenesis-related (PR) proteins: Proteins encoded by the host plant
but induced in pathological situations. The PR proteins are induced by various types of pathogens as well as stress conditions.
pathotoxin: The toxin that is a major determinant of disease and pathogenicity. A pathotoxin induces all the typical disease symptoms at a physiological concentration and is correlated with pathogenicity. Host-specific
toxins are considered to be pathotoxins.
pathotype: A subdivision of species distinguished by common characteristics of pathogenicity, particularly in relation to host range.
pathovars: (see FORMA SPECIALIS) Pathovars and formae speciales are
synonymous. Formae speciales in bacteria are known as pathovars.
PCR (polymerase chain reaction): A method for the selective amplification of DNA sequences.
pectate lyases (PL or Pel) and pectin lyases (PTE): They induce lytic degradation of the glycosidic linkage, resulting in an unsaturated bond between
carbons 4 and 5 of a uronide moiety in the reaction product. Pectate lyases
act on polygalacturonic acids, while pectin lyases act on pectins.
pectic acid (or polygalacturonic acid): Refers to the nonesterified forms,
while pectinic acid (or pectin) refers to partially esterified forms of pectic
substances (see PECTINS).
pectin methyl esterase (PME): The enzyme that catalyzes the conversion
of pectin by de-esterification of the methyl ester group with production of
methanol. PME removes the methoxyl group from rhamnogalacturonan
chains. PME is more active on methylated oligogalacturonates than on pectin.
pectinic acids: See PECTINS.
pectins: -1,4-Galacturonans with various degrees of methyl esterification.
The unesterified uronic acid molecules containing up to 75 percent of
methoxyl groups are known as pectinic acid, and those with more than 75
percent of methyl groups are known as pectins. Generally the pectinic acids
are called pectins.
penetrants: Wetting agents, oils, or oil concentrates that enhance the absorption of a systemic fungicide by the plant.
perfect state: Stage in the life cycle of a fungus characterized by sexual
spores.
perithecium: The ascocarp is more or less closed, but at maturity it is provided with a pore through which the ascospores escape.
peritrichous: Having flagella distributed over the whole surface.
persistent viruses: The virus-vector relationship varies widely depending
on the duration of the virus in the vector (persistence). In case of persistent
viruses, the virus may simply circulate through the body of the vector or
may propagate as well. Hence, this relationship can be classified as (1) noncirculative nonpersistent, (2) noncirculative semi-persistent, (3) circulative
nonpropagative, and (4) circulative propagative transmission.
physiologic race: A taxon of parasites, particularly characterized by specialization to different cultivars of one host species. Physiologic races and
formae speciales do not differ morphologically. Within formae speciales
races may exist. Bacteriologists use the term pathovar or pathotype, which
are both comparable in definition to formae speciales and race in fungi.
phytoalexin: Plant antibiotics that are synthesized de novo after the plant
tissue is exposed to microbial infection. Phytoalexins are also defined as
low-molecular-weight, antimicrobial compounds that are both synthesized
by and accumulated in plants after exposure to microorganisms. Two important criteria have been suggested to label a plant secondary metabolite a
phytoalexin: (1) the secondary metabolite should be produced de novo in response to infection, and (2) the compound should accumulate to antimicrobial concentrations in the area of infection.
phytoanticipins: Low-molecular-weight, antimicrobial compounds present in plants before challenge by microorganisms and/or produced after infection solely from preexisting constituents.
phytoncide: A chemical substance produced by plants that can inhibit the
growth of microorganisms.
phytosanitary certificate: A certificate of health that accompanies plants
or plant products to be exported.
phytotoxic: Toxic to plants.
phytotoxin: Toxin produced by pathogens that is toxic to plants but not considered to be of primary importance during pathogenesis. Nonspecific toxins are considered to be phytotoxins.
plant activators: The chemicals that activate the defense genes by providing signals.
plant pathology: Study of plant disease.
plasmid: A circular extrachromosomal element and a circular DNA molecule. A plasmid is not essential for cell growth. It is used as a vector of the
recombinant DNA in genetic engineering studies.
plasmodium: A naked (wall-less) motile mass of protoplasm with many
nuclei, bounded by a plasma membrane. It moves and feeds in an amoeboid
fashion (it engulfs food and feeds by ingestion).
plasmogamy: In this phase, the union of two protoplasts takes place, bringing nuclei close together within the same cell.
polycyclic: Disease having more than one cycle of infection during a growing season.
polygenic resistance: The resistance is governed by several genes.
polysomes: Ribosomes act in clusters and are called polyribosomes or
polysomes. Ribosomes are the sites of protein synthesis.
population: A group of organisms of the same species occupying a particular space at a particular time. A population is a pool of individuals from
which the next generation will be drawn. The spatial and temporal limits of
a population are defined by uniform allele frequencies.
promoter: DNA sequence lying upstream from a gene to which RNA polymerase binds.
propagule: That part of an organism by which the organism may be dispersed or reproduced.
prophylaxis: Preventative treatment against disease.
prosenchyma: During certain stages of fungal development, the mycelium
becomes organized into loosely or compact woven tissues, as against the
loose hyphae ordinarily found in the mycelium. The loosely woven tissue in
which the component hyphae with elongated cells lie more or less parallel to
one another is called prosenchyma.
protectant fungicide: A fungicide that protects against invasion by a
pathogen.
protozoa: A kingdom characterized by the presence of organisms that are
predominantly unicellular, plasmodial, or colonial. These organisms are
phagotrophic, i.e., they feed by ingestion, engulfing food.
pseudoparenchyma: In certain stages of fungal development, fungal tissues are closely packed, in the form of more or less isodiametric or oval cells
resembling the parenchyma cells of higher plants, and they are called
pseudoparenchyma.
pycnidium: An asexual globose or flask-shaped hollow fruiting body lined
with conidiophores (plural: pycnidia).
pyramiding of genes: Combining different genes in a single plant.
qualitative resistance: The disease symptom is almost completely suppressed, mostly by development of hypersensitive pinpoint fleck reaction.
quantitative resistance: The resistance is based on the amount of disease
symptom development, quantified by different infection types such as necrotic flecks, necrotic and chlorotic areas with restricted sporulation, sporulation with chlorosis, abundant sporulation without chlorosis, lesion size,
lesion area, etc.
quantitative trait loci (QTL): The genetic loci associated with complex
traits.
quarantine: Legislative or regulatory control that aims to exclude pathogens from areas where they do not already exist. It includes the holding of
imported material in isolation for a period to ensure freedom from diseases
and pests.
race: A taxon of pathogens, particularly characterized by specialization to
different cultivars of one host species.
race-nonspecific/nonspecific resistance: The resistance is not specific to

only a particular race.
race-specific resistance/specific resistance: The resistance is specific to
certain races of the pathogen.
recessive gene: A gene that is phenotypically manifest in the homozygous
state but is masked in the presence of its dominant allele or dominant gene
(see DOMINANT GENE).
resistant: Possessing qualities that prevent or retard the development of a
given pathogen.
restriction enzyme: An endonuclease that cuts DNA at sites defined by its
recognition sequence.
RFLP (restriction fragment-length polymorphism): A variation in the
locations of restriction sites in DNA of different individuals; the fragment
length defined by the restriction sites may be of different lengths in different
individuals.
rhizomorph: The mycelium of some fungi forms thick strands. In such
strands, the hyphae lose their individuality and form complex tissues. The
strands are called rhizomorph (rootlike structures). The strand has a thick
hard cortex and a growing tip. It looks like a root tip.
ribosomes: Globular structures found in the cytoplasm. They are composed
of about one-third protein and two-thirds RNA. Ribosomes are designated
30s, 50s, 70s, etc., depending on their size. The size is determined by the
rate, measured in svedberg unit(s), at which a particle sediments when it is
centrifuged at high speed in an ultracentrifuge.
satellite RNA: Satellite RNA has no coat protein. It completely depends on
viruses for multiplication.
satellite virus: A satellite virus has a coat of its own but has only a small genome. It is dependent on a helper virus for its replication. It needs another
virus to supply replicase and probably other enzymes. It encapsidates its
RNA within its own coat protein. It acts as a parasite of plant-parasitic viruses.
sclerotium: A hard and compact vegetative resting structure of fungi. It
is mostly made up of pseudoparenchymatous tissue (see PSEUDOPARENCHYMA).
seedling resistance: Resistance detectable only at the seedling stage.
sense codon: Any of the 61 triplet codons in mRNA that specify an amino
acid.
sense strand: In duplex DNA, the strand that serves as a template for the
synthesis of RNA; it is also known as the anticoding strand.
sigma factor: A polypeptide subunit of RNA polymerase. Sigma factors
are composed of two functional domains: a core-binding domain and a
DNA-binding domain.
simple-interest disease: A disease that goes through only one cycle of infection during a growing season, analogous to a bank account giving simple
interest.
simulation models: Simulation is the process of designing a model of a real
system and conducting experiments with this model for purposes of either
understanding the behavior of the system or evaluating various strategies for
the operation of the system. Simulation models are generated by creating
conditions for development of epidemics and carrying out experiments on
different aspects of disease increase.
somaclonal variation: The stable and heritable variation displayed among
somaclones.
somaclones: Plants regenerated from cell, tissue, and organ cultures.
sorus: A mass of spores (plural: sori).
Southern blotting: A method to detect DNA fragments by electrophoresis.
species: A species includes strains of fungi or bacteria, with approximately
70 percent or greater DNA-DNA relatedness or less Tm (divergence [unpaired bases] within related nucleotide sequences is 5 percent or less).
sporangiophore: A specialized hyphal branch bearing sporangia.
sporangiospore: A nonmotile asexual spore produced in a sporangium.
sporangium: A sac-like structure in which its entire contents are converted
into one or more spores, which are called sporangiospores.
spore: A specialized propagative or reproductive body.
sporodochium: An asexual fruiting body in which the conidiophores are
cemented together and the conidiophores arise from the surface of a cushionshaped stroma.
spreader: A substance added to a spray to assist in its even distribution over
the target.
statistical models: Epidemiological models that are mathematical formulas with values of parameters chosen to adequately describe disease progress for specific data sets but lacking a precise biological interpretation.
sticker: A substance added to a spray to assist in its adhesion to the target.
straggling: Breakage of a few plant stems, especially cereals, resulting in a
few tillers falling down.
stroma: A fungal structure, usually made up of prosenchyma (see PROSENCHYMA). On or in the stroma, fructifications (see FRUCTIFICATIONS) are
formed (plural: stromata).
subspecies: Based on minor but consistent phenotypic variations within the
species or on genetically determined clusters of species. Subspecies designations are used for genetically close organisms that diverge in phenotype.
superrace: A pathogen race that contains virulence factors to match any resistance factors available in the host.
surfactant: A surface-active material, especially a wetter or spreader used
with a spray.
susceptible: Subject to infection; nonimmune.
symplast: The sum total of living protoplasm of a plant including the
phloem and protoplast. The total mass of living cells of a plant constitute a
continuum, the individual protoplasts being ultimately connected throughout the plant by plasmodesmata.
synnema: A group of conidiophores cemented together forming an elongated spore-bearing structure (plural: synnemata).
systemic fungicide: A fungicide that is absorbed and translocated in the
plant.
taxa: Taxonomic groups of any rank (singular: taxon).
taxonomy: Systematic classification.
teleomorph: See ANAMORPH.
thyriothecium: An inverted ascocarp having the wall more or less radial in
structure.
tolerant: Able to endure infection by a pathogen without showing severe
symptoms of disease.
trans-acting element: A genetic element that can exert its effect without
having to be on the same molecule as a target sequence; this element encodes an enzyme or regulatory protein that can diffuse to the site of action.
transposons: Mobile DNA segments that can insert into a few or several
sites in a genome. They are transposable genetic elements (the word transpose means alter the positions of or interchange). They are also called
jumping genes.
true resistance: The resistance is complete.
vacuoles: Cavities in the cytoplasm containing a fluid called cell sap.
vector: An organism that transmits a pathogen.
vertical resistance: Resistance that is specific to some races of the pathogen and that will be susceptible to other races.
virion: The complete and infectious nucleoprotein particle of the virus.
viroids: Naked nucleic acids without a coat protein. They consist of only ribonucleic acid (RNA). These miniviruses are the smallest known causal
organisms of infectious diseases.
viruses: Infectious agents not visible under the microscope (submicroscopic) and small enough to pass through a bacterial filter.
virusoids: Viruses that contain a viroid-like satellite RNA in addition to a
linear single-stranded molecule of genomic RNA.
vivotoxin: The toxin produced in vivo (in the infected tissues) that functions in disease development but not as a primary agent.
volunteer plant: A self-sown plant.
volutins: Metachromatic granules found in bacterial cells that localize in
the vacuoles of mature forms. They contain inorganic polyphosphate, lipoprotein, RNA, and magnesium and may serve as phosphate storage structures.
Western blotting: A method to detect protein molecules by electrophoresis.
Index
Acervulus, 35, 569
Actinomycetes, 13
Adhesion
bacterial cells, 551
cutinases, 551
extracellular polysaccharides, 552
fimbriae, 551
fungal spores, 551
glycoprotein, 551
hydrophobins, 551
lipopolysaccharides, 552
pili, 551
rhicadhesin, 552
vitronectin-like proteins, 552
Adjuvants, 354, 355, 356, 569
Agglutinin, 569
Agrobacterium radiobacter
Agrocin, 84, 257
bacteriocin, 257
biocontrol, 257
crown gall control, 257
Die-gall, 259
NoGall, 259
Norbac 84-C, 259
Alfalfa
cyst nematode (Heterodera trifolii),
85
dagger nematode (Xiphinema
americanum), 85
leaf and stem nematode
(Ditylenchus dipsaci), 84
needle nematode (Longidorus spp.),
86
needle nematode (Rotylenchulus
spp.), 86
pin nematode (Paratylenchus
hamatus), 86
pin nematode (Paratylenchus spp.),
86
root-knot nematode (Meloidogyne
arenaria), 87
chitwoodi), 88
Alfalfa (continued)
hapla), 88
incognita), 88
javanica), 88
root lesion nematode (Pratylenchus
neglectus), 88
penetrans), 88
spp.), 88
seedborne pathogens, 434
spiral nematode (Helicotylenchus
spp.), 89
stubby-root nematode
(Paratrichodorus spp.), 89
stunt nematode (Tylenchorhynchus
spp.), 90
Almond
americanum), 85
rivesi), 86
ring nematode (Criconemella
xenoplax), 87
arenaria), 87
incognita), 88
javanica), 88
vulnus), 88
spp.), 90
Alternaria alternata
citrus brownspot, 213
conditions favoring spore release,
213
Alternate host, 569

Alternative host, 569
Ampelomyces quisqualis
AQ10, 259
biocontrol, 259
Amphitrichous, 569
Analytic models, 569
Anamorph, 47, 569
Anisogramma anomala
conditions favoring ascospores
release, 213
European hazelnut pathogen, 213
Antheridium, 32, 35, 570
Antibiotic, 570
Antibodies, 570
Anticodon, 570
Antigen, 570
Antiserum, 570
Apiosporina morbosa
213
sour cherry black knot, 213
Aplanospore, 46
Apoplast, 570
Apothecium, 37, 570
Apparent infection rate, 209, 570
Apple
americanum), 85
rivesi), 86
vuittenezi), 86
fire blight, 14
list of diseases, 467, 468, 469, 470
86
xenoplax), 87
spp.), 87
penetrans), 88
spp.), 88
Apple fire blight forecasting models
Billing Revised System (BRS), 230
Billing System, 230
Billings Integrated System (BIS95),
230
Apple fire blight forecasting models

(continued)
Cougarblight (Smith) Fire Blight
Risk Assessment Model, 230
FireWork, 229
MARYBLYT, 229
Mean Temperature Line model, 230
Appressorium, 570
Apricot
americanum), 85
rivesi), 86
xenoplax), 87
arenaria), 87
javanica), 88
vulnus), 88
Area under disease progress curve
(AUDPC), 205, 208
Arthrospores, 34
Ascocarp, 570
Ascoconium, 35, 36, 38
Ascospore, 570
Ascostroma, 571
Ascus, 571
Ascus mother cell, 36
AUDPC, 285, 570
Aureobasidium pullulans
biocontrol, 253
competition, 253
induced resistance, 253
Autoecious rust, 571
Avirulence genes, 252, 284, 553, 571
Avirulent, 571
Avocado red rust/algal spot, 73
Awl nematodes, 84
Dolichodorus heterocephalus, 84
Dolichodorus spp., 84
Bacillus cereus
apple crown gall control, 257
biocontrol, 256, 257
Bacillus polymyxa
Antirrhinum rust control, 257
biocontrol, 257
rose powdery mildew control, 257
Bacillus subtilis
Actizyme, 258
BACT-0, 256
Bactophyt, 259
biocontrol, 256
Epic, 259
FZB24, 256, 258
Kodiak, 259
Kodiak (A-13), 259
System 3 (GBO3), 259
Bacteria, 571
Bactericides, 333-336
Bacteriophages, 13, 571
Banana
Banana bunchy top virus, 152
list of diseases, 470, 471, 472
moko wilt, 14
Panama wilt, 166
arenaria), 87
incognita), 88
brachyurus), 88
coffeae), 88
goodeyi), 88
reniformia), 88
dihystera), 89
multicinctus), 89
Barley
cyst nematode (Heterodera avenae),
85
cyst nematode (Heterodera
filipjevi), 85
latipons), 85
list of diseases, 472, 473, 474, 475
root gall nematode (Subanguina
radicicola), 87
artiellia), 88
chitwoodi), 88
naasi), 88
Barley (continued)
spp.), 87
spp.), 88
seed health testing methods, 440
stunt nematode (Merlinius
brevidens), 90
stunt nematode (Merlinius spp.), 90
dubius), 90
maximus), 90
Barley yellow dwarf virus, 152
Barley yellow mosaic virus, 152
Basidiocarp, 41, 571
Basidiospore, 40, 571
Basidium, 41, 571
Bean
bacterial brown spot, 13
common blight, 14
glycines), 85
americanum), 85
halo blight, 13
lance nematode (Hoplolaimus spp.),
86
list of diseases, 475-476
86
reniform nematode (Rotylenchulus
reniformis), 87
ring nematode (Criconemoides
ovantus), 87
arenaria), 87
hapla), 88
incognita), 88
javanica), 88
penetrans), 88
spp.), 88
Bean (continued)
dihystera), 89
sting nematode (Belonolaimus
longicaudatus), 89
stubby-root nematode (Paratrichodorus christiei), 89
stunt nematode (Quinisulcius
acutus), 90
spp.), 90
Beet
schachtii), 85
cyst nematode (Heterodera trifolii),
85
false root-knot nematode (Nacobbus
aberrans), 86
86
potato rot nematode (Ditylenchus
destructor), 87
spp.), 87
spp.), 88
stubby-root nematode (Trichodorus
spp.), 90
Beijerinck, Martinus Willem, 3
Binary fission, 13
Biological control of nematodes, 94
Bitunicate ascus, 36
Blumeria graminis
conditions favoring spore release, 212
wheat powdery mildew, 212
Bock, Jerome, 2
Boom-bust cycle, 285
Botrytis cinerea
conditions favoring infection, 220
conditions favoring sporulation, 212
strawberry gray mold, 212
systematic position, 47
Bremia lactucae
213
downy mildew of lettuce, 31
Broadbean
Orobanche aegyptica, 79
Orobanche cernua, 79
Burrill, Thomas Jonathan, 3
Cabbage
black rot, 15
seed health testing methods, 441,
442
Cacao red rust/algal spot, 73, 75
Caius Plinius Secundus, 337
Calluses, 325, 327
Candidatus Liberobacter africanum
Citrus greening disease, 14, 18
Candidatus Liberobacter asiaticum, 14,
18
Citrus greening disease, 14, 18
Capsule, 11
Carolus Linnaeus, 2
Carrot
carotae), 85
lance nematode (Hoplolaimus
uniformis), 86
hapla), 88
spp.), 88
penetrans), 88
longicaudatus), 89
Cassytha filiformis, 80
Catch crops, 423
Cauliflower
black rot, 15
cDNA, 571
Celery
awl nematode (Dolichodorus
heterocephalus), 84
awl nematode (Dolichodorus spp.),
84
cyst nematode (Heterodera spp.), 85
hamatus), 86
86
spp.), 88
sting nematode (Belonolaimus spp.),
89
Cellulase, 557, 558
Cell wall modifications
appositions, 511
callose, 511
cell wall reinforcement, 511
disease resistance, 512
lignification, 511
papilla, 511, 512, 513
papilla regulating extract, 513
vesicles, 512
Cell wall proteins
arabinogalactan proteins, 515
extensins, 515
hydroxyproline-rich proteins, 515
lectins, 515
proline-rich proteins, 515
Cephaleuros virescens
algal spot, 73
avocado, 73
cacao, 73
citrus, 73
coffee, 73
guava, 73
litchi, 73
mango, 73
oilpalm, 73
papaya, 73
pecan, 73
pepper, 73
red rust, 73
sapota, 73
tea, 73
vanilla, 73
Cercospora arachidicola
conditions favoring disease
development, 220
peanut early leaf spot, 212
Chickpea Fusarium wilt, 166
Chlamydospores, 571
Chondrioid, 12
Christian Hendrik Persoon, 3
Chromatin body, 12
Chromista, 29, 30, 48, 571
Chromosome, 11, 571
cis-acting element, 571
Citrus
canker, 13
citrus greasy spot (Mycosphaerella
citri), 166, 167
citrus nematode (Tylenchulus
semipenetrans), 85
dagger nematode (Xiphinema spp.),
85
greening, 14,18
list of diseases, 477-478
Loranthus, 80
86
postbloom fruit drop and
anthracnose forecasting
model, 230
red rust/algal spot, 73, 75
spp.), 87
spp.), 88
sheath nematode (Hemicycliophora
arenaria), 89
longicaudatus), 89
stubborn, 14
spp.), 90
tristeza, 152
variegated chlorosis, 14
Clamp connection, 41
Classification of Ascomycota
Dictionary of Fungis classification,
37, 38
FungalWebs classification, 39, 40
Classification of Basidiomycota
44, 45
FungalWebs classification, 41, 42,
43
Classification of Chytridiomycota, 46
Classification of Mitosporic fungi, 47,
48
Classification of Oomycota
30, 31
FungalWebs classification, 31
Classification of Phytoplasmas
Apple proliferation group (16SrX),
102
Ash yellows group (16SrVII), 102
Aster Yellows group (16SrI), 100
Bermuda grass white leaf group
(16SrXIV), 103
Clover proliferation group (16SrVI),
102
Coconut lethal yellows group
(16SrIV), 101
Elm yellows group (16SrV), 101
Loofah witches-broom group
(16SrVIII), 102
Mexican periwinkle virescence
group (16SrXIII), 103
Peanut witches-broom group
(16SrIII), 101
Pigeon pea witches- broom group
(16SrIX), 102
Rice yellow dwarf group (16SrXI),
102
Stolbur group (16SrXII), 102
X-disease group (16SrIII), 101
Classification of Zycomycota, 46, 47
Clavibacter michiganensis subsp.
sepedonicus
potato ring rot, 15
Claviceps sorghi
sorghum ergot, 212
Cleidemus, 1
Cleistothecium, 37, 571
Coconut Hartrot, 72
Codon, 572
Coenocytic, 46, 572
Coffee
phloem necrosis, 71
red rust/algal spot, 73
spp.), 87
Colletotrichum acutatum
conditions favoring spore dispersal,
214
strawberry anthracnose, 215
Colletotrichum gloeosporioides
Glomerella cingulata, 254
reduced pathogenicity isolates, 25
Complementary genes, 572
Complementation test, 572
Complete resistance, 572
Compound interest diseases, 206
Computer-based decision support
systems
AUPNUT, 396
BLITECAST, 396
CU-FAST, 396
DESSAC, 396
EPIPRE, 395
FAST, 396
Firework, 396
MARYBLYT, 396
MoreCrop, 395
PRO_PLANT, 395
TOM-CAST, 396
WDCA, 395
Conidiophore, definition, 572
Conidium, definition, 572
Coniothyrium minitans
biocontrol, 254
Coniothyrin, 254
Contans, 254
Conjugation, 13
Copy number, 572
Corn
awl nematode (Dolichodorus
heterocephalus), 84
awl nematode (Dolichodorus spp.), 84
brown stripe downy mildew, 31
burrowing nematode (Radopholus
similes), 85
corn downy mildew, 31
crazy-top downy mildew, 31
85
Corn (continued)
cyst nematode (Heterodera zeae), 85
cyst nematode (Punctodera
chalcoensis), 85
americanum), 85
mediterraneum), 86
dorsalis), 86
green ear and downy mildew, 31
columbus), 86
galeatus), 86
lance nematode (Hoplolaimus spp.),
86
list of diseases, 478, 479, 480, 481,
482
needle nematode (Longidorus
breviannulatus), 86
86
Philippine downy mildew, 31
ornate), 87
ring nematode (Criconemella spp.),
87
chitwoodi), 88
incognita), 88
javanica), 88
spp.), 87
brachyurus), 88
crenatus), 88
hexincisus), 88
neglectus), 88
penetrans), 88
scribneri), 88
Corn (continued)
thornei), 88
zeae), 88
spp.), 88
southern corn blight
(Helminthosporium maydis),
170
spp.), 89
Stewarts disease, 15
longicaudatus), 89
89
Striga, 77
(Paratrichodorus christiei),
89
(Paratrichodorus minor), 90
spp.), 90
acutus), 90
dubius), 90
Corn Stewarts wilt forecasting model,
234
Cosmid, 572
Cotton
bacterial blight (black arm), 15
schachtii), 85
americanum), 85
columbus), 86
list of diseases, 482, 483
africans), 86
hamatus), 86
reniformis), 87
Cotton (continued)
87
incognita), 88
spp.), 88
spp.), 89
spiral nematode (Scutellonema
spp.), 89
longicaudatus), 89
spp.), 90
Cowpea
Striga, 77
Crop rotation, 93, 425
Crucifers
84
club root, 33
cruciferae), 85
schachtii), 85
86
spp.), 87
pratensis), 88
seed health testing methods, 441,
442
89
white rust, 31
Cucurbits
cucumber Monosporascus wilt, 167
cucumber mosaic virus, 152
americanum), 85
downy mildew, 31
Cucurbits (continued)
86
reniformis), 87
87
spp.), 87
spp.), 88
spp.), 89
longicaudatus), 89
claytoni), 90
Cuscuta, 80, 81
Cutinases, 554, 555
Cyst nematode
Globodera pallida, 85
Globodera rostochiensis, 85
Globodera solanacearum = G.
virginiae, 85
Globodera tabacum, 85
Heterodera avenae, 85
Heterodera carotae, 85
Heterodera cruciferae, 85
Heterodera filipjevi, 85
Heterodera glycines, 85
Heterodera hordecalis, 85
Heterodera latipons, 85
Heterodera schachtii, 85
Heterodera spp., 85
Heterodera trifolii, 85
Heterodera zeae, 85
Punctodera chalcoensis, 85
Cytoplasmic inclusion bodies, 12, 572
Dagger nematode, 85
Xiphinema americanum, 85
Xiphinema brevicolle, 85
Dagger nematode (continued)

Xiphinema diversicaudatum, 85
Xiphinema index, 86
Xiphinema mediterraneum, 86
Xiphinema rivesi, 86
Xiphinema spp., 85
Xiphinema vuittenezi, 86
De Bary, Heinrich Anton, 3
Defective interfering virus, 124
Diagnosis of bacterial diseases
Agrobacterium tumefaciens, 192
Agrobacterium vitis, 192
michiganensis, 192
sepedonicus, 192
Erwinia amylovora, 192
Erwinia carotovora, 192
Erwinia chrysanthemi, 192
Pantoea stewartii, 192
Pseudomonas savastanoi pv.
phaseolicola, 192
Pseudomonas syringae pv.
morsprunorum, 192
Pseudomonas syringae pv. tomato,
192
Ralstonia solanacearum, 192
Xanthomonas axonopodis pv. citri,
192
Xanthomonas axonopodis pv.
phaseoli, 192
Xanthomonas campestris pv.
campestris, 192
Xanthomonas translucens pv.
undulosa, 192
Xanthomonas vesicatoria, 192
Diagnosis of fungal diseases
Botrytis cinerea, 191
Colletotrichum acutatum, 190
Fusarium culmorum, 190
Fusarium oxysporum f. sp. narcissi,
190
Fusarium spp., 191
Gaeumannomyces graminis, 190,
191
Leptosphaeria korrae, 191
Leptosphaeria maculans, 190
Magnaporthe poae, 191
Phiolophora gregata, 191
Phytophthora capsici, 190
Diagnosis of fungal diseases

(continued)
Phytophthora cinnamomi, 190
Phytophthora citrophthora, 190
Phytophthora fragariae, 190
Phytophthora infestans, 190
Phytophthora palmivora, 190
Phytophthora parasitica, 190
Phytophthora spp., 190
Pseudocercosporella
herpotrichoides, 190, 191
Pyrenophora graminea, 190
Pyrenophora teres, 190
Pythium spp., 190
Pythium ultimum, 191
Rhizoctonia oryzae, 191
Rhizoctonia spp., 190
Rhynchosporium secalis, 191
Spongospora subterranea, 190
Stagonospora nodorum, 190
Stagonospora tritici, 190
Tilletia controversa, 190
Verticillium dahliae, 190
Verticillium spp., 191
Diagnosis of phytoplasma diseases
Ash Y phytoplasma, 195
Peach eastern X-disease, 196
Diagnosis of viral diseases
Apple mosaic virus, 194
Banana bract mosaic virus, 193
Citrus tatter leaf virus, 194
Citrus tristeza virus, 192
Grapevine rupestris stem pittingassociated virus, 194
Groundnut ringspot virus, 193
Impatiens necrotic spot virus, 193
Peanut mottle virus, 194
Peanut stripe virus, 194
Prune dwarf virus, 194
Prunus necrotic ringspot virus, 194
Tomato chlorotic spot virus, 193
Tomato spotted wilt virus, 193
Watermelon silverleaf mottle virus,
193
Diagnosis of viroid diseases
apple scar skin viroid, 195
bidirectional electrophoresis, 195
chrysanthemum stunt viroid, 195
citrus exocortis viroid, 195
hop stunt viroid, 195
Diagnosis of viroid diseases

(continued)
molecular hybridization, 195
peach latent mosaic viroid, 195
pear rusty skin viroid, 195
RT-PCR, 195
Differential variety, 572
Disease assessment
aerial IR-color photograph, 170
area under disease progress curve
(AUDPC), 169
banana Panama wilt, 166
bell pepper bacterial spot
(Xanthomonas campestris pv.
vesicatoria), 167
category value, 165
chickpea Fusarium wilt, 166
citrus greasy spot (Mycosphaerella
citri), 166, 167
cucumber Monosporascus wilt, 167
digital image analysis, 169, 170
disease index, 169
disease severity, 166
pear brown spot (Stemphylium
vesicatarium), 165, 166, 168
percentage disease incidence, 165
phytopathometry, 165
remote sensing, 169, 170
rice sheath blight (Rhizoctonia
solani), 168
scale value, 165
southern corn blight (Helminthosporium maydis), 170
soybean sudden death syndrome
(Fusarium solani f. sp.
glycines), 167
tomato Fusarium wilt, 166
vertical disease progress, 168
Weber-Fechner law, 166
wheat leaf blotch, 168
Disease definition, 1, 572
Disease diagnosis
agglutination test, 177
antibodies, 174, 193
antigen, 174, 175
antigen-capture indirect ELISA, 178
bidirectional electrophoresis, 195
colony blot, 186, 192
DAPI test, 195
DASI-ELISA, 192
Disease diagnosis (continued)

Dienes stain, 195
digitally assisted diagnosis, 196, 197
dip-stick ELISA, 180
direct sandwich ELISA, 179
direct tissue blot immunoassay, 180,
193
DNA hybridization, 195
dot-blot diagnosis, 185, 186
dot-blot ELISA, 180
double antibody sandwich ELISA,
179, 192, 193, 194
ELISA, 176, 178, 183, 192, 196
F(ab')2- based ELISA, 178, 179
gas chromatography, 192
hybridization formats, 184, 185
hybridoma cells, 176, 193
immunoassay kits, 190
immunoassays, 174
immunocapture PCR, 188, 189, 195
immunocapture-reverse
transcription-PCR (IC-RTPCR), 189
immuno-electrophoresis, 178
immunofluorescence, 182, 193
immunogens, 175
immunogold assay, 183, 193
immunosorbent dilution-plating
technique, 183
immunosorbent electron
microscopy, 183, 193
indirect enzyme-linked ImmunoPCR, 188
indirect enzyme-linked
immunosorbent assay, 192
In situ hybridization assay, 186
In situ immunoassay (ISIA), 181
latex-agglutination test, 177
latex-Protein A agglutination assay,
182
molecular hybridization, 195
monoclonal antibodies, 176, 177,
184, 193, 196
nested PCR, 192
nucleic acid probe-based methods,
184
Ouchterlony immunodiffusion test,
177
PCR, 186, 187, 188, 196
Disease diagnosis (continued)

Disease resistance types (continued)
PCR dot-blot, 192
general resistance, 278
PCR-ELISA, 192
horizontal resistance, 278
major-gene resistance, 277, 278, 289
phage display technology, 181
minor gene resistance, 278
plant clinics, 196
monogenic resistance, 277, 278
plant disease diagnostic service
laboratories, 196
nonspecific resistance, 278
polyclonal antibodies, 175, 177, 193
partial resistance, 278, 286
polyclonal antisera, 175
polygenic resistance, 278
precipitation test, 177
qualitative resistance, 277, 286
radioimmunoassay, 184, 193
quantitative resistance, 278, 285
RAPD, 187
race-nonspecific resistance, 278
race-specific resistance, 277, 289
recombinant enzyme-linked
immunosorbent assay, 181
specific resistance, 277
repetitive-sequence-based PCR (reptrue resistance, 277, 278
PCR), 189, 191, 192
vertical resistance, 277
reverse-blot hybridization method,
Disease-specific genes, 572
192
Disease symptoms
RFLP, 192, 195
anthracnose, 157
RT-PCR, 194, 195, 196
areolate mildew, 157
triple antibody sandwich ELISA,
big vein, 157
179
black rust, 161
visual inspection, 173
black scurf, 157
Western blotting, 183, 193
blackening, 157
Disease forecasting models
blast, 157
analytic models, 225
blight, 157
empirical models, 226
blister blight, 157
hybrid models, 226
boll rot, 157
model, definition, 225
brown rot, 157
simulation models, 226
brown rust, 161
Disease gradients, 218, 219
browning, 157
Disease progress curve, 206, 207, 572
bud blight, 158
Disease resistance breeding methods
bud rot, 158
backcross breeding, 283
bunchy top, 158
bulk population selection, 283
bushy stunt, 158
pedigree selection, 283
canker, 158
recurrent selection, 283
charcoal rot, 158
Disease resistance genes
chlorosis, 158
additive effect, 279, 286
club root, 158
cloning, 293, 294, 295
collar rot, 158
dominant gene, 279
covered kernel smut, 162
epistasis, 279
covered smut, 162
major gene, 287, 289
crown gall, 158
minor gene, 287, 289
crown rot, 158
recessive gene, 279
curly top, 158
suppressors, 279, 280
damping-off, 158
transgenic plants, 293, 294, 295
die-back, 158
Disease resistance types
downy mildew, 158
dry rot, 158
complete resistance, 277, 286
dwarf, 159
field resistance, 278
Disease symptoms (continued)

ear rot, 159
enation, 159
etch, 159
false smut, 159
fern-leaf, 159
fire blight, 159
flag smut, 162
fleck, 159
flower color-breaking, 159
freckle, 159
fruit rot, 159
gray mold, 159
green ear, 159
gummosis, 160
head rot, 160
head smut, 162
kernel rot, 160
kernel smut, 162
leaf blight, 160
leaf blotch, 160
leaf crinkle, 160
leaf crumple, 160
leaf curl, 160
leaf roll, 160
leaf spots, 160
line pattern, 160
little leaf, 160
long smut, 162
loose smut, 162
mosaic, 160
mottle, 160, 161
necrosis, 161
phyllody, 161
powdery mildew, 161
pox, 161
red rot, 161
reddening, 161
rhizome rot, 161
ringspot, 161
root rot, 161
rosette, 161
rot diseases, 161
rugose, 161
rusts, 161
scab, 162
scald, 162
shot-hole, 162
silver leaf, 162
smut, 162
Disease symptoms (continued)

soft rot, 162
sooty mold, 162
spindle tuber, 163
spotted wilt, 163
stalk rot, 163
stem bleeding, 163
stem gall, 163
stem rot, 163
stenosis, 163
sterility, 163
storage rot, 163
streak, 163
stripe, 163
stripe rust, 162
stunt, 163
tumours, 163
vein band, 163
vein chlorosis, 163
vein clearing, 163
white rust, 162
wilt, 164
witches broom, 164
yellow rust, 161
yellows, 164
Disease threshold, 397
Disease triangle, 205
Diseases list
apple, 467, 468, 469, 470
banana, 470, 471, 472
barley, 472, 473, 474, 475
bean, 475, 476
citrus, 477, 478
corn, 478, 479, 480, 481, 482
cotton, 482, 483
crucifers, 483, 484, 485
cucurbits, 485, 486, 487
grape, 487, 488, 489, 490
oats, 490, 491
pea, 492
peach, 492, 493, 494
peanut, 494, 495, 496
pear, 497, 498
plum, 498, 499
potato, 499, 500, 501
rice, 501, 502
tomato, 502, 503, 504
wheat, 505, 506, 507, 508
Dispersing agents, 354
Dodder. See Cuscuta
Dolipore septa, 41
Dominant gene, 573
Dot-blot, 573
Dot-blotting, 573
Duhamel du Monceau, Henri Louis, 2
Durable resistance
additive minor genes, 290
definition, 288, 573
factors contributing, 289
major gene resistance, 289
pyramiding of genes, 290
quantitative resistance, 289
race-specific resistance, 289
Exclusion, 431-433
Exopolygalacturonase, 574
F factor, 13
Fabricius, Johann Christian, 2
Facultative parasite, 574
Facultative saprophyte, 574
False root-knot nematodes
Nacobbus aberrans, 86
Nacobbus dorsalis, 86
Fimbriae, 13
Fission, 35
Flagellate protozoa
Herpetomonas, 71
Phytomonas, 71
Egg fungi, 30
Flagellum
Electrolyte leakage, 552
definition, 574
Elicitors, definition, 573
peritrichous, 12
Empirical models, 573
polar, 12
Emulsifiers, 354, 573
Foam suppressors, 354, 572
Endemic, 205, 573
Fontana, 2
Endopolygalacturonase, 573
Forma specialis (f. sp.), 574
Enhancer, 573
Forsyth, William, 337
Epidemic, 205, 208, 211, 220, 573
Fructification, 574
Epidemiology, 205, 573
Fumigation, 574
Episome, 11, 13, 574
Fungi, definition, 574
Epistasis, 574
Fungicidal, 574
Eradication, 433, 434
Fungicide, definition, 574
Erwinia amylovora
Fungicide application equipments
apple fire blight, 14
air blast sprayer, 405, 406
pear fire blight, 15
air jet sprayer, 405, 406
backpack sprayer, 404
Erwinia carotovora subsp. atroseptica,
bucket sprayer, 404
15
compressed air sprayer, 404
potato blackleg and bacterial soft
drenching equipments, 409
rot, 15
dust seed treater, 407
electrostatic sprayer, 406
Erwinia carotovora subsp. carotovora
estate sprayer, 405
fruit tree injector, 408
rot, 15
granule applicators, 407
gravity-fed applicator, 409
tomato bacterial stem rot and fruit
hand dusters, 403
rot, 16
high-pressure fumigator, 409
Erwinia chrysanthemi pv. chrysanthemi
high-pressure hydraulic sprayer, 405
hose-end hand sprayer, 404
rot, 15
low-pressure boom sprayer, 405
low-pressure boomless sprayer, 405
Erysiphe graminis; see Blumeria
graminis
Mist-O-Matic seed treater, 408
Eukaryota, 29
Panogen liquid treater, 407
Fungicide application equipments

(continued)
parts of applicators, 409, 410, 411
power dusters, 403
power wheelbarrow sprayer, 405
pressure-fed applicator, 409
push-pull hand sprayer, 404
slurry seed treater, 407
soil fumigators, 408
soil incorporators, 408
soil injectors, 408
spinning disc sprayer, 406
trembone sprayer, 404
trigger pump sprayer, 404
tunnel sprayer, 406
ultralow volume sprayer, 406
Fungicide formulations
aqueous solution, 363
aqueous suspension, 363
coating agent, 367
driftless dust, 359
dry flowables, 361
dry or slurry seed treatment
formulations, 364, 365
dustable powder, 358
emulsifiable concentrate, 359
emulsifiable seed treatment
formulations, 366
flowable concentrate, 360
flowable formulations, 360
flowable liquid, 361
flowable seed treatment, 366
flowable suspension, 360
fumigants, 366
liquid fungicide, 363
liquid seed treatment formulations,
365
microgranule, 363
oil dispersible powder, 363
oil-miscible liquid, 364
oily formulation, 364
paste formulation, 364
soluble concentrate, 360, 361
suspension concentrate, 361
ULV formulation, 364
water-dispersible granule, 362
water miscible concentrate, 361
water-soluble bag, 362
water-soluble granule, 362
water-soluble powder, 359
wettable powder, 357
Fungicide resistance
cross-resistance, 399
development causes, 399
management, 400
Fungicides
chemical names, 341-349
common names, 341-349
diseases controlled, 381-394
fungicide families, 368-370
ready formulated mixtures, 349-353
surface protectant fungicides, 370
systemic fungicides, 367, 370
trade names, 341-349
Fungicides, mode of action
acylalanines, 377
aminoacid amide carbamates, 381
anilinopyrimidines, 380
benzimidazoles, 377
blasticidin S, 379
butylamine, 374
carboxamides, 378
carpropamid, 379
chlorinated nitroanilines, 375
chloroneb, 380
chlorophenyls, 373
copper compounds, 371
cymoxanil, 380
dicarboximides, 381
dicloran, 380
dimethomorph, 381
dithiocarbamates, 372, 373
edifenphos, 376
famoxadone, 379
fosetyl-aluminium, 381
guanidines, 374
hexachlorobenzene, 380
imidazoles, 375
iprofenphos, 376
isoprothiolane, 376
isoxazoles, 378
kasugamycin, 379
mercury compounds, 372
morpholines, 375
nitro compounds, 375
organochlorines, 375
organophosphorous compounds, 380
pentachlorophenol, 380
phenylpyrroles, 380
phthalimides, 373
Fungicides, mode of action (continued)

piperazines, 375
piperidines, 375
polyoxins, 379
propamocarb, 380
pyridines, 375
pyrimidines, 375
pyroquilon, 379
quinines, 373, 374
quinoxalines, 374
quinoxyfen, 380
spiroxamines, 375
strobilurins, 378
sulfur, 371
sulphamides, 374
tecnazene, 380
thiadiazoles, 374
tin compounds, 372
triazines, 374
triazoles, 375
tricyclazole, 379
validamycin, 376
Fungistatic, 574
Fusarium solani f. sp. glycines
disease severity assessment, 167
soybean sudden death syndrome, 167
Gametoclonal variation, 329
Gaussian model, 207
Gene, definition, 11, 575
Gene cloning, 575
Gene-for-gene hypothesis, 284, 575
General resistance, 575
Genetic engineering, 575
Germ tube, definition, 575
Gliocladium catenulatum
diseases controlled, 251, 252
PreStop, 251
Primastop, 252
Glycogen, 12
Gompertz model, 207
Grape
citrus nematode (Tylenchulus semipenetrans), 85
dagger nematode (Xiphinema americanum), 85
index), 86
85
Grape (continued)
downy mildew, 31
list of diseases, 487, 488, 489, 490
86
87
spp.), 88
vulnus), 88
spp.), 87
Grapevine downy mildew forecasting
models
empirical models, 231
EnviroCaster, 231
Green bridge, 575
Groundkeeper, 575
Guava
Loranthus, 79
Hales, Stephen, 2
Haustorium, 575
Helminthosporium maydis
remote sensing, 170
southern corn blight, 170
Helper virus, 123, 575
Hemibiotroph, 575
Hemicellulases, 558
Heteroecious rust, 575
Heterothallism, 32, 46, 575
Holomorph, 576
Homothallism, 32, 576
Hooke, Robert, 2
Horizontal resistance, 576
Host-selective toxin, 559, 576
Host-specific resistance, 559, 560, 576
Host-specific virulence genes, 576
hrp genes, 552, 553, 576
Hydrophobic particle films, 426
Hydroxyproline-rich proteins (HRGPs)
accumulation in cell wall, 515
agglutination, 517
cell wall strengthening, 516
cross-linking, 516
insolublization of HRGPs, 516
role in disease resistance, 515, 516,
517
Hymenium, 36, 576

Hyperparasitism, 576
Hyperplasia, 576
Hypersensitivity, 576
Hypertrophy, 576
Hypha, definition, 576
Hyphopodium, 576
Hypoplasia, 577
Hypoviruses
biocontrol, 255
chestnut blight control, 256
Cryphonectria parasitica, 255
dssRNA viruses, 255
Immune, 577
Imperfect state, definition, 577
In vitro selection
among regenerants, 326
at callus stage, 327, 328
from organ cultures, 328
from somatic hybrids, 328, 329
Incubation period, 577
Induced systemic resistance
definition, 445
systemic acquired resistance, 445
Infection, definition, 577
Infection court, 577
Infection peg, 577
Inhibitins, 577
Inoculum, 577
Inoculum potential, 577
Inorganic amendmends, 424
Intercellular, 577
Intercropping, 425
Intracellular, 577
Irrigation on disease severity, 426
Karyogamy, 32, 33, 36, 577

Kenrick, William, 338
Knight, Thomas Andrew, 338
Koch, Robert, 3
Kochs postulates, 577
Khn, Julius Gotthelf, 3
Lance nematodes
Hoplolaimus columbus, 86
Hoplolaimus galeatus, 86
Hoplolaimus spp., 86
Hoplolaimus uniformis, 86
Latent period, 577
Leptodontium elatius
apple sooty blotch, 211
Lettuce
downy mildew, 31
Lignin
biosynthesis, 519, 520
role in disease resistance, 520, 521
Linnaeus, Carolus, 2
List of crop diseases. See Diseases list
Living ground covers, 422
Lodging, 578
Logistic model, 206, 207
Loranthus
casuarina, 80
kapok, 80
mango, 80
mulberry, 80
pomegranate, 80
rosewood, 80
rubber, 80
sandalwood, 80
teak, 80
Maize. See Corn

Major gene, 578
Major-gene resistance, 578
Malpighi, Marcello, 2
Mango
brevicolle), 85
columbus), 86
Loranthus, 79
sheathoid nematode
(Hemicriconemoides
mangiferae), 86
Mango anthracnose forecasting models,
233, 234
Marker-assisted selection, 280, 281,

282
Mastigoneme, 30, 578
Mayer, Adolph, 3
Meiosis, 32, 33, 578
Mesosome, 12
Messenger RNA (mRNA), 578
Micheli, 2
Microbial pesticides
commercially available products,
258, 259, 260
Microconidia, 37
Microsporangium, 75
Microzoospore, 75
Middle lamella, 578
Millardet, Alexis, 3
Millardet, Pierre, 338
Minor-gene resistance, 578
Minor genes, 578
Mitosis, 36, 47
Molecular markers
AFLP, 280, 281, 282
CAPS, 282
PCR, 280, 281
RAPD, 280, 281, 282
RFLP, 280, 281
SCAR, 280, 281, 282
SSR, 280, 281
STS, 280, 281
usefulness in breeding, 283
Monocyclic disease, 206, 578
Monogenic resistance, 578
Monomolecular model, 206
Monosporascus cannonballus
development, 220
cucurbit Monosporascus root rot,
220
Monotrichous, 578
Mulching, 417, 422
Multiline mixtures
clean crop, 290
definition, 578
dirty crop, 290
Murein, 12
Mutation breeding, 288
Mycelium, definition, 578
Mycolaminarin, 30
Mycorrhiza
arbuscular mycorrhiza, 271
commercialization, 274
ectendomycorrhiza, 271
ectomycorrhiza, 271
endomycorrhiza, 271
vesicular-arbuscular mycorrhiza, 271
Mycorrhizal fungi
diseases controlled, 272
mass production, 273, 274
mycorrhizal symbiosis, 272
Mycosphaerella citri
ascospore production, 211
citrus greasy spot, 166, 167
disease severity assessment, 166, 167
Mycosphaerella fijiensis
banana black leaf streak (black
Sigatoka), 211
215
212, 213
Mycosphaerella musicola
banana Sigatoka (yellow Sigatoka),
211
Mycotoxin, 578
Near-isogenic lines (NILs), 283, 287

Necrotroph, definition, 578
Needle nematodes
Longidorus africans, 86
Longidorus breviannulatus, 86
Longidorus spp., 86
Rotylenchulus spp., 86
Nematicides
aldicarb, 95
carbofuran, 96
chloropicrin, 95
D-D, 96
dazomet, 96
diazinon, 96
dibromochloropropane, 96
dichlofenthion, 96
dichloropropene, 96
Nematicides (continued)
ethoprophos, 96
ethylene dibromide, 96
fenamiphos, 96
fensulfothion, 96
metham-sodium, 97
methyl bromide, 97
methylisothiocyanate, 97
oxamyl, 97
terbufos, 97
thionazin, 97
Nematodes, disease symptoms
coarse root, 92
curly top, 91
devitalized root, 92
hairy root, 91
root knots, 91
root lesions, 91
root rot, 91
root surface necrosis, 92
stubby root, 91
Nematodes, structure, 83, 84
Nonpathogenic Fusarium
biocontrol, 254
Biofox-C, 259
Fusaclean, 259
Fusarium oxysporum, 254
Nonpathogenic Ralstonia
biocontrol, 254
PSSOL, 259
Ralstonia solanacearum, 254
Nonpathogenic Rhizoctonia
biocontrol, 254
Sclerotinia sclerotiorum, 254
Nonspecific toxin, 559, 579
Northern blotting, 579
Notifiable disease, 579
Oats
85
hordecalis), 85
latipons), 85
Oats (continued)
chalcoensis), 85
americanum), 85
87
ring nematode (Nothocriconemella
mutabilis), 87
chitwoodi), 88
naasi), 88
spp.), 87
spp.), 88
thornei), 88
spp.), 89
spp.), 89
longicaudatus), 89
spp.), 90
Obligate parasite, 579
Oidia, 34
Oilpalm
Marchitez disease, 72
Phytomonas, 72
Oogonium, 32, 579
Operon, 579
Organ cultures, 328
Organic amendments, 419-422
Orobanche
broadbean, 79
melon, 79
sunflower, 79
Orobanche (continued)
tobacco, 79
tomato, 79
trap crop, 79
Palladius, 2
Pantoea agglomerans
apple fire blight, 257
biocontrol, 257
honey bees, 257
pear fire blight control, 257
Pantoea stewartii ssp. stewartii
corn Stewarts disease, 15
Papaya red rust/algal spot, 73
Papilla formation
calcium ion, 513
callose, 511
disease resistance, 512
factors responsible for, 513
papilla-regulating extract, 513
phosphate salts, 513
Paraphyses, 36
Parasexual cycle, 579
Parasite, 579
Pathogen, definition, 579
Pathogenesis-related proteins
chitonases, 530
classification, 525
definition, 523, 524, 579
glycine-rich protein, 530
PR-1 proteins, 525
PR-2 proteins, 525
PR-3 proteins, 526
PR-4 proteins, 527
PR-5 proteins, 527
PR-6 proteins, 527, 528
PR-7 proteins, 528
PR-8 proteins, 528
PR-9 proteins, 528, 529
PR-10 proteins, 529
PR-11 proteins, 529
PR-12 proteins, 529
PR-13 proteins, 529
PR-14 proteins, 530
ribosome-inactivating proteins, 530
532, 533
synergistic action, 533, 534
Pathotoxin, 559, 579

Pathotype, definition, 579
Pathovars, definition, 579
PCR, definition, 579
Pea
list of diseases, 492
Pea seedborne mosaic virus, 152
root rot, 31
Peach
bacterial canker, 15
americanum), 85
rivesi), 86
vuittenezi), 86
necrotic ring spot virus, 153
xenoplax), 87
arenaria), 87
incognita), 88
javanica), 88
spp.), 87
penetrans), 88
vulnus), 88
Peanut
86
pod lesion nematode
(Tylenchorhynchus
brevilineatus = T.
brevicadatus), 87
ornate), 87
arenaria), 87
hapla), 88
javanica), 88
Peanut (continued)
brachyurus), 88
coffeae), 88
seed and pod nematode (Ditylenchus
destructor), 88
cavenessi), 89
gracilis), 89
longicaudatus), 89
testa nematode (Aphelenchoides
arachidis), 90
Peanut early and late leaf spots
forecasting models
81-ADV, 232
89-ADV, 232
AU-Pnuts advisory, 232
Early Leaf Spot Advisory, 232
EnviroCaster, 232
Pear
brown spot (Stemphylium
vesicatarium), 165, 166, 168
americanum), 85
rivesi), 86
vuittenezi), 86
fire blight, 15
spp.), 87
penetrans), 88
spp.), 88
Pear brown spot forecasting model
BSPcast, 233
empirical model, 233
Stemphylium vesicarium, 233
Pecan red rust/algal spot, 73
Pectate lyases, 579
Pectic acid, 579
Pectic enzymes, 555, 556, 557
Pectin, 580
Pectin lyases, 579
Pectin methyl esterase (PME), 580

Pectinic acids, 579
Peltaster fructicola
apple sooty blotch, 211
Penetrants, 580
Peniophora gigantea
biocontrol, 259
Pg suspension, 259
Phlebia gigantea, 259
Pepper red rust/algal spot, 73
Peptidoglycan, 12
Perfect state, definition, 580
Perithecium, 37, 580
Peritrichous, 580
Persistent viruses, 580
Petrus Crescentius, 2
Phaeoisariopsis personata
peanut late leaf spot, 212
Phagotrophic, 30
Phenolics
539
soluble phenolics, 537
wall-bound phenolics, 537, 539, 540
Phialophora
biocontrol, 255
wheat take-all control, 255
Physiologic race, definition, 580
Phytoalexins
antimicrobial action, 544
role in disease resistance, 545, 546
Phytoanticipins, 581
Phytomonas
hart rot of coffee, 72
marchitez disease of oilpalm, 72
phloem necrosis of coffee, 71
Phytoncide, 581
Phytophthora cactorum
crown and root rot of apple, 30
crown and root rot of peach, 30
Phytophthora infestans
hetrothallism, 32
late blight of potato, 30
late blight of tomato, 30
Phytophthora palmivora
citrus brown fruit rot, 211
Phytoplasmas
classification, 100
description, 99
disease diagnosis, 104
disease management, 104
symptoms
big bud, 103
blackening, 103
bushy stunt, 103
die back, 103
leaf curling, 103
leaf roll, 103
little leaf, 103
phyllody, 103
reddening, 103
slender shoots, 103
sterility, 103
stunting, 103
witches-broom, 103
yellows, 103
transmission, 103, 104
Phytosanitary certificate, 581
Phytotoxic, 581
Pili, 13
Pin nematodes
Paratylenchus hamatus, 86
Paratylenchus spp., 86
Pittonde Tournefort, Joseph, 2
Plant activators
aluminium chloride (Synermix),
453
BABA, 450, 451
BTH, 448-450
Carpropamid, 454
INA, 446-448
jasmonic acid, 454
KeyPlex, 453
methyl jasmonate, 454
Myco-Sin, 454
phosphate, 454
Phytogard (K2HPO3), 454
plant defense activators, 445
practical uses, 455
Probenazole, 451, 452
Prohexadione-Ca, 452
riboflavin, 453
salicylic acid, 451
Ulmasud, 454
Plant extracts
antimicrobial action, 461
antiviral proteins, 463
induction of resistance, 462, 463
Plant pathology, definition, 581
Plant propagation materials indexing,
442, 443
Plasmid, 11, 581
Plasmodium, 33, 581
Plasmogamy, 32, 33, 36, 581
Plint, 1
Plum
bacterial spot, 15
plum pox (Sharka) virus, 153
Polycyclic disease, 206, 581
Polygenic resistance, 581
Polyribosome, 12
Polysome, 12, 581
Population, 581
Potato
bacterial wilt (brown rot), 15
blackleg (bacterial soft rot), 15
common scab, 15
cyst nematode (Globodera pallida),
85
cyst nematode (Globodera
rostochiensis), 85
aberrans), 86
leafrolling, 153
mild mosaic, 153
Potato mop top virus, 153
potato rot nematode (Ditylenchus
destructor), 87
Potato virus A, 153
Potato virus M, 153
Potato virus X, 153
Potato virus Y, 153
powdery scab, 33
ring rot, 15
hapla), 88
incognita), 88
Potato (continued)
javanica), 88
spp.), 87
brachyurus), 88
penetrans), 88
spp.), 88
rugose mosaic, 153
Potato late blight forecasting models
Beaumont period, 227
BLITECAST, 227, 228
Dutch rules, 226, 227
Irish rules, 227
NEGFRY, 227
SIM-CAST, 228
Smith period, 227
Prevost, Benedict, 3, 338
Progametangium, 46
Prokaryotes, 11
Promoter, 581
Propagule, 582
Prophylaxis, 582
Prosenchyma, 34, 582
Proteases, 558, 559
Protectant fungicide, definition, 582
Protozoa, 29, 30, 32, 48, 582
Pseudocercosporella herpotrichoides
wheat eyespot, 212
Pseudofungi, 29
Pseudomonas savastanoi pv. glycinea
soybean bacterial blight, 15
Pseudomonas savastanoi pv.
phaseolicola
bean halo blight, 14
Pseudomonas spp. as biocontrol agents
antibiotic production, 249
BioCoat, 259
biocontrol efficacy, 250
Biosave-100, 258
Biosave-110, 258
Blue Circle, 259
Conqueror, 259
Dagger, 259
Pseudomonas spp. as biocontrol agents

(continued)
Deny, 259
diseases controlled by
Pseudomonas, 245, 246, 251
-1,3-glucanase, 250
hydrocyanic acid, 250
induced systemic resistance, 246,
247, 248, 249
Intercept, 259
method of application, 251
Pseudomonas aeruginosa, 247
Pseudomonas aureofaciens, 251
Pseudomonas cepacia
(Burkholderia cepacia), 245
Pseudomonas fluorescens, 245, 246,
247, 248, 249
Pseudomonas putida, 245, 246
siderophore, 250
Pseudomonas syringae pv. syringae
bean bacterial spot, 14
peach bacterial canker, 15
Pseudoparenchyma, 34, 582
Pseudothecium, 37
Puccinia graminis f. sp. tritici
incidence, 220
214
wheat stem rust, 214
Puccinia triticina
conditions favoring urediniospore
dispersal, 214
conditions favoring urediniospores
release, 212
wheat leaf (brown) rust, 212
Pycnidium, 35, 582
Pyramiding of genes
backcrosses, 287
defeated genes, 287, 288
durable resistance, 289
major genes, 287
minor genes, 287
NILs, 287
RFLP, 287
Pythium oligandrum
elicitins, 252
Polygandron, 252, 259
Richards model, 207

Ring nematodes
Criconemella spp., 87
Criconemella axesta, 87
Criconemella ornata, 87
Criconemella xenoplax, 87
Criconemoides ovantus, 87
Nothocriconemella mutabilis, 87
Robertson, John, 338
Race, definition, 582
Root-knot nematodes
Race-nonspecific resistance, definition,
Meloidogyne arenaria, 87
583
Meloidogyne artiellia, 88
Race-specific resistance, definition, 583
Meloidogyne chitwoodi, 88
Recessive resistance, 583
Meloidogyne hapla, 88
Recombinant, 14
Meloidogyne incognita, 88
Registration of fungicides,
Meloidogyne javanica, 88
greenhouse tests, 339
Meloidogyne naasi, 88
laboratory tests, 339
Meloidogyne spp., 87
toxicological data, 340
Root lesion nematodes
Reniform nematodes
Pratylenchus brachyurus, 88
Rotylenchulus reniformis, 87
Pratylenchus coffeae, 88
Rotylenchus spp., 87
Pratylenchus crenatus, 88
Resistant, definition, 583
Pratylenchus goodeyi, 88
Restriction enzyme, 583
Pratylenchus hexincisus, 88
RFLP, definition, 583
Pratylenchus minyus, 88
Rhizoctonia solani
Pratylenchus neglectus, 88
disease severity assessment, 168
Pratylenchus penetrans, 88
rice sheath blight, 168
Pratylenchus pratensis, 88
Rhizomorph, 34, 583
Pratylenchus reniformia, 88
Ribavirin, 413
Pratylenchus scribneri, 88
Ribosomes, 12, 583
Pratylenchus spp., 88
Rice
Pratylenchus thornei, 88
bacterial blight, 15
Pratylenchus vulnus, 88
bacterial leaf streak, 15
Pratylenchus zeae, 88
crimp nematode (Aphelenchoides
Rose
besseyi), 85
grassy stunt virus, 153
diversicaudatum), 85
axesta), 87
rice sheath blight (Rhizoctonia
hapla), 88
solani), 168
Rice tungro virus, 153
penetrans), 88
spp.), 87
vulnus), 88
root nematode (Hirschmaniella
oryzae), 87
nannus), 89
spiral nematode (Rotylenchus spp.),
stem nematode (Ditylenchus
89
angustus), 89
Qualitative resistance, definition, 582
Quantitative resistance, definition, 582
Quantitative trait loci, 284, 285, 286,
582
Quarantine, 92, 431, 432, 433, 582
Seed health testing methods

Rose (continued)
(continued)
acutus), 90
immuno-diffusion test, 439, 440
immunofluorescence assay, 439,
spp.), 90
440, 441
Rye
immunomagnetic separation, 440, 442
immunosorbent electron
85
microscopy, 439, 440, 441
indicator host inoculation, 440, 441
indicator plants, 440, 441, 442
root gall nematode (Subanguina
latex agglutination test, 439, 440
radicicola), 87
microprecipitin test, 439
plating enriched seed extract, 440
spp.), 87
polymerase chain reaction (PCR),
seed gall nematode (Anguina tritici),
439, 441, 442
89
radio immunosorbent assay, 439
repetitive-sequence-based
polymerase chain reaction,
440
Sanitation, 93, 417, 418
seed extract and dilution plating,
Sapota red rust/algal spot, 73
439, 441
Satellite RNA, 5, 123, 583
seed washing method, 438, 440
Satellite virus, 5, 123, 583
seedling inoculation test, 439
Sclerotinia sclerotiorum
seedling symptom test, 439, 442
Cabbage stem rot, 213
serological test, 439
conditions favoring apothecium
sodium hydroxide soak test, 440
production, 211
Seedborne pathogens, 434, 435, 436
Seedling resistance, 583
213
Sense codon, 584
soybean stem rot, 211, 212
Sense strand, 584
Sclerotium, 34, 583
Septoria nodorum. See Stagonospora
Seed health testing, 436, 437
nodorum
Seed health testing methods
Septoria tritici
2,4-D blotter test, 440, 442
agar-fluorescence test, 440
development, 220
agar tests, 439, 442
blotter tests, 438, 442
215
deep freezing test, 440, 442
pycnidiospore production, 211
dilution plating, 440, 441
wheat Septoria speckled leaf blotch,
DNA hybridization, 439, 441
211
dot-immunobinding assay, 439, 440,
Serratia marcescens
441
biocontrol, 258
embryo staining test, 439, 440
lettuce Sclerotinia control, 258
enzyme-linked fluorescent assay,
Serratia plymuthica
439
biocontrol, 258
enzyme-linked immunosorbent
assay (ELISA), 439, 440, 441,
Sheath nematode
442
Hemicycliophora arenaria, 89
growing-on test, 439, 440
Hemicycliophora spp., 89
grow-out test, 439, 442
Sigma factor, 584
immunoblot test, 439, 440, 441
Simple interest diseases, 206, 584

Simulation model, 584
Slime layer, 11
Soil amendmends, 93, 94
Soil pH, 424
Soil solarization, 417, 419
Somaclonal variation, 326, 327, 329,
330, 584
Somaclones, 325, 326, 330, 584
Somatic hybrids, 328, 329
Sorghum
84
americanum), 85
africans), 86
86
reniform nematode (Rotylenchus
spp)., 87
87
spp.), 87
spp.), 88
spp.), 89
longicaudatus), 89
Striga, 77
spp.), 90
Sorus, 584
Southern blotting, 584
Soybean
bacterial blight, 15
glycines), 85
columbus), 86
galeatus), 86
reniformis), 87
Soybean (continued)
ornate), 87
arenaria), 87
hapla), 88
incognita), 88
javanica), 88
spp.), 88
spp.), 89
spp.), 89
gracilis), 89
longicaudatus), 89
sudden death syndrome (Fusarium
solani f. sp. glycines), 167
Species, definition, 584
Spermatia, 37
Spermatiophore, 37
Spiral nematodes
Helicotylenchus dihystera, 89
Helicotylenchus multicinctus, 89
Helicotylenchus nannus, 89
Helicotylenchus spp., 89
Rotylenchus spp., 89
Scutellonema cavenessi, 89
Scutellonema spp., 89
Spiroplasma citri
citrus stubborn, 14, 104, 105
transmission, 105
Spiroplasma kunkelli
corn stunt, 105
symptoms, 105
transmission, 105
Spiroplasmas, 104, 105
Sporangiophore, 584
Sporangiospore, 584
Sporangium, 31, 46, 584
Spore, definition, 583
Spore dispersal gradient, 218
Spore samplers
Burkard, 216, 217
Cascade impactor, 216
Cyclone, 216, 217
Funnel, 216, 217
Hirst, 216
Impinger, 216
Rotorod, 216, 217
spore traps, 215
Spore traps. See Spore samplers
Sporodochium, 35, 584
Spreader, 584
Stagonospora nodorum
conditions favoring pycnidiospores
dispersal, 215
conditions favoring pycnidiospores
release, 212
Septoria nodorum, 212
wheat leaf and glume blotch, 212
Statistical models, 585
Stemphylium vesicatarium
disease incidence assessment, 165
pear brown spot, 165
Sterigmata, 35
Stickers, 354, 585
Sting nematodes
Belonolaimus gracilis, 89
Belonolaimus longicaudatus, 89
Belonolaimus spp., 89
Straggling, 585
Strawberry
americanum), 85
85
hapla), 88
spp.), 87
coffeae), 88
penetrans), 88
pratensis), 88
scribneri), 88
gracilis), 89
Strawberry (continued)
longicaudatus), 89
Strawberry gray mold forecasting
models, 232
Straggling, 585
Streptomyces scabies, 15
incidence, 220
potato common scab, 15
Streptomyces griseoviridis
biocontrol, 258
cauliflower alternaria control, 258
Mycostop, 259
pepper damping-off control, 258
Striga
corn, 77
cowpea, 77
rice, 77
sorghum, 77
Striga asiatica, 77
Striga gesnerioides, 77, 78
Striga hermonthica, 78
sugarcane, 77
tobacco, 77
trap crops, 79
Stroma, 34, 585
Structure of viruses
Alfalfa mosaic virus (AMV), 112
Andean potato latent virus (APLV),
112
Andean potato mottle virus
(APMV), 112
Arabis mosaic virus (ArMV), 112
Barley stripe mosaic virus (BSMV),
112
Barley yellow dwarf virus (BYDV),
112
Barley yellow mosaic virus
(BaYMV), 112
Barley yellow striate mosaic virus
(BYSMV), 112
Bean common mosaic virus
(BCMV), 112
Bean golden mosaic virus (BGMV),
112
Bean pod mottle virus (BPMV), 112
Bean rugose mosaic virus (BRMV),
112
Structure of viruses (continued)

Glycine mottle virus (GmoV), 114
Bean yellow mosaic virus (BYMV),
Grapevine fan leaf virus (GFLV),
112
Beet leaf curl virus (BCLV), 112
114
Beet mosaic virus (BtMV), 113
Lettuce mosaic virus (LMV), 114
Beet western yellows virus
Lettuce necrotic yellows virus
(BWYV), 113
(LNYV), 114
Beet yellow stunt virus (BYSV), 113
Maize chlorotic dwarf virus
Beet yellows virus (BYV), 113
(MCDV), 114
Black raspberry latent virus
Maize mosaic virus (MMV), 114
(BRLV), 113
Maize streak virus (MSV), 114
Broad bean stain virus (BBSV), 113
Muskmelon vein necrosis virus
Broad bean true mosaic virus
(MkVNV), 114
(BBTMV), 113
Narcissus yellow stripe virus
Brome mosaic virus (BMV), 113
(NYSV), 114
Carnation Italian ring spot virus
Oat blue dwarf virus (OBDV), 114
(CIRSV), 113
Oat mosaic virus (OMV), 115
Carnation latent virus (CLV), 113
Oat striate mosaic virus (OSMV),
Carnation necrotic fleck virus
115
(CNFV), 113
Onion yellow dwarf virus (OYDV),
Carnation ringspot virus (CRSV),
115
113
Papaya mosaic virus (PapMV), 115
Carnation vein mottle virus
Papaya ringspot virus (PRSV), 115
Parsnip mosaic virus (ParMV), 115
(CVMV), 113
Pea early browning virus (PEBV),
Carrot latent virus (CaLV), 113
Carrot red leaf virus (CaRLV), 113
115
Carrot thin leaf virus (CTLV), 113
Pea enation mosaic virus (PEMV),
Carrot yellow leaf virus (CYLV),
115
113
Pea leaf roll virus (PeLRV), 115
Cauliflower mosaic virus (CaMV),
Pea seed-borne mosaic virus
113
(PSMV), 115
Celery mosaic virus (CeMV), 113
Pea streak virus (PeSV), 115
Cherry leafroll virus (CLRV), 113
Peach rosette mosaic virus
Chloris striate mosaic virus
(PRMV), 115
(CSMV), 113
Peanut mottle virus (PeMoV), 115
Citrus leaf rugose virus (CiLRV),
Peanut stunt virus (PSV), 115
114
Peanut yellow mottle virus (PYMV),
Citrus tristeza virus (CTV), 114
115
Citrus variegation virus (CVV), 114
Pepino latent virus (PeLV), 115
Clover yellow mosaic virus
Pepper mottle virus (PepMoV), 115
(ClYMV), 114
Pepper veinal mottle virus (PVMV),
Clover yellow vein virus (ClYVV),
115
114
Plum pox virus (PPV), 115
Clover yellows virus (CYLV), 114
Potato black ring spot virus
Cowpea aphid-borne mosaic virus
(PBRV), 115
(CABMV), 114
Potato leaf roll virus (PLRV), 116
Cowpea mosaic virus (CPMV), 114
Potato virus A (PVA), 116
Cucumber mosaic virus (CMV), 114
Potato virus M (PVM), 116
Cymbidium mosaic virus (CybMV),
Potato virus S (PVS), 116
Potato virus X (PVX), 116
114

Potato virus Y (PVY), 116
Potato yellow dwarf virus (PYDV),
116
Prune dwarf virus (PDV), 116
Prunus necrotic ringspot virus
(PNRSV), 116
Raspberry ringspot virus (RRV),
116
Red clover necrotic mosaic virus
(RCNMV), 116
Red clover vein mosaic virus
(RCVMV), 116
Rice black-streaked dwarf virus
(RBSDV), 116
Rice dwarf virus (RDV), 116
Rice necrosis mosaic virus
(RNMV), 116
Rice ragged stunt virus (RRSV),
116
Rice transitory yellowing virus
(RTYV), 116
Rice tungro bacilliform virus
(RTBV), 116
Rice tungro spherical virus (RTSV),
116
Rice yellow mottle virus (RYMV),
116
Shallot latent virus (SLV), 117
Southern bean mosaic virus
(SBMV), 117
Sowbane mosaic virus (SoMV), 117
Soybean dwarf virus (SoyDV), 117
Soybean mosaic virus (SoyMV),
117
Subterranean clover mottle virus
(ScMoV), 117
Subterranean clover red leaf virus
(SCRLV), 117
Sugarcane mosaic virus (SCMV),
117
Sweet clover necrotic mosaic virus
(SCNMV), 117
Tobacco etch virus (TEV), 117
Tobacco mosaic virus (TMV), 117
Tobacco necrosis virus (TNV), 117
Tobacco necrotic dwarf virus
(TNDV), 117
Tobacco rattle virus (TRV), 117

Tobacco ring spot virus (TRSV),
117
Tobacco streak virus (TSV), 117
Tobacco vein mottling virus
(TVMV), 117
Tomato aspermy virus (TAV), 117
Tomato black ring virus (TBRV),
117
Tomato bushy stunt virus (TBSV),
117
Tomato golden mosaic virus
(ToGMV), 118
Tomato mosaic virus (ToMV), 118
Tomato ringspot virus (ToRSV), 118
Tomato spotted wilt virus (TSWV),
118
Tulip breaking virus (TBV), 118
Turnip mosaic virus (TuMV), 118
Turnip rosette virus (TroV), 118
Turnip yellow mosaic virus
(TYMV), 118
Watermelon mosaic virus (WMV),
118
Wheat chlorotic streak virus
(WCSV), 118
Wheat yellow leaf virus (WYLV),
118
White clover mosaic virus
(WCIMV), 118
Stubby-root nematodes
Paratrichodorus christiei, 89
Paratrichodorus minor, 90
Paratrichodorus spp., 89
Trichodorus spp., 90
Stunt nematodes
Merlinius brevidens, 90
Merlinius spp., 90
Quinisulcius acutus, 90
Tylenchorhynchus claytoni, 90
Tylenchorhynchus dubius, 90
Tylenchorhynchus maximus, 90
Tylenchorhynchus spp., 90
Subspecies, definition, 585
Sugarcane
downy mildew, 31
spp.), 87
spp.), 88
Sugarcane (continued)
spp.), 89
spiral nematode (Rotylenchus spp.),
89
spp.), 89
Striga, 77
Sunflower
Super race, 585
Surfactants, 354, 585
Susceptible, definition, 585
Sweet potato white rust, 31
Symplast, 585
Synnema, 35, 585
Systematic position
bacterial pathogens, 18-27
fungal pathogens, 48-69
viruses, 130-140
Systemic fungicide, definition, 585
Tobacco (continued)
americanum), 85
foliar nematode (Aphelenchoides
ritzemabosi), 86
reniformis), 87
arenaria), 87
hapla), 88
incognita), 88
javanica), 88
brachyurus), 88
penetrans), 88
spp.), 88
Talaromyces flavus
spp.), 89
glucose oxidase, 254
Striga, 77
Penicillium dangeardii, 253
Taphrina deformans
peach leaf curl, 220
spp.), 90
Taxa, 585
Taxonomy, definition, 585
Tea red rust/algal spot, 73, 74, 75
spp.), 90
Teleomorph, 47
Tobacco mosaic virus, 153
definition, 585
Tolerant, 585
Theopharstus, 1
Tomato
Thyriothecium, 585
Tillage, 423
bacterial speck, 15
Tillet, Mathieu, 2
bacterial spot, 15
Tilletia indica
bacterial stem rot and fruit rot, 16
bacterial wilt, 16
wheat Karnal bunt, 219
Fusarium wilt, 166
Tobacco
solanacearum = G. virginiae),
85
spp.), 87
tabacum), 85
Tomato (continued)
longicaudatus), 89
spp.), 90
Tomato mottle virus, 153
Tomato spotted wilt virus, 153
Tomato early blight forecasting models
FAST, 228
TOM-CAST, 229
Toxins
role in pathogenesis, 562, 563, 564
types of toxins, 559, 560, 561
Tozzetti, 2
Trans-acting element, 586
Transgenic plants
alfalfa, 296, 298
apple
bacterial disease resistance, 308
fungal disease resistance, 306
Arabidopsis
fungal disease resistance, 299
bean, 311
carrot, 310
cauliflower, 309
commercial availability, 312, 313
corn, 311
cucumber, 303
Nicotiana accidentalis, 304
Nicotiana benthamiana
viral disease resistance, 302, 303,
304, 305, 306
oilseed rape, 293, 296
peanut, 303
pear, 308, 311
Petunia hybrida, 306
potato
bacterial disease resistance, 299,
300, 301, 308-310
fungal disease resistance, 296,
299, 300, 309
304, 306
Transgenic plants (continued)

rice
299, 307
viral disease resistance, 303
rose, 296
squash, 302
sugarcane, 311, 312
tamarillo, 303
tobacco, 294, 296-303
297, 300, 301, 308-311
fungal disease resistance,
296-300, 309
viral disease resistance, 297,
302-304, 306, 307, 312
tomato
296
298, 300, 301
wheat
307
303
Transposon, 11, 586
Trap crops, 94
Trichoderma
1295-22, 259
Agro-Lig, 241
antibiotic production, 244
ANTI-FUNGUS, 260
application methods, 241
ATC 20847, 259
Binab-T, 242
BINAB-T and W, 259
Bip T, 260
bumble bees, 242
combination with mycorrhiza, 242
competition, 245
diseases controlled by Trichoderma,
241, 242, 243
effect of time of application, 242
enzyme inactivation, 245
F-stop, 259
GlioGard, 259
Harzian 10, 259
Harzian 20, 259
Trichoderma (continued)
honey bees, 242
Hors-solsain, 259
KRLAG2, 259
mixures of Trichoderma, 242
mycoparasitism, 243
osmopriming, 241
Plantsain, 259
Promot, 259
Rootshield, 239, 241
SoilGard, 259
solid matrix priming, 241
Solsain, 259
Supraavit, 259
T-22B, 259
T-22G, 259
Topshield, 239
Trichoderma formulations, 240, 241
Trichoderma harzianum, 239, 240,
241, 242, 244
Trichoderma koningi, 240
Trichoderma polysporum, 242
Trichoderma spp., 239
Trichoderma viride, 242
Trichodermin, 259
Trichodex, 259
Trichodowels, 242
Trichoject, 259
Trichopel, 259
Trichoseal, 259
Tri-D25, 258
Ty, 259
Trichogyne, 36
True fungi, 29
True resistance, 586
Ulocladium atrum
biocontrol, 255
Botrytis cinerea control, 255
Uncinula necator
213
grapevine powdery mildew, 213
Unitunicate ascus, 36
UV filters, 354
Vacuoles, 12, 13, 586

Van Leeuwenhoek, Antoni, 2
Vanilla red rust/algal spot, 73
Varro, 1
Vector, definition, 586
Venturia inaequalis
apple scab, 214
214, 215
Venturia pirina
development, 220, 221
pear leaf scab, 220
Vertical resistance, 586
Verticillium lecanii
biocontrol of Spherotheca fuliginea,
252
parasitism on Penicillium digitatum,
252
parasitism on Puccinia striiformis,
252
Viricides, 413
Virion, 586
Virioplasm, 123
Viroids
Apple scar skin viroid, 107
Australian grapevine viroid, 107
Avocado sunblotch viroid, 107
Chrysanthemum chlorotic mottle
viroid, 107
Chrysanthemum stunt viroid, 107
Citrus exocortis viroid, 107
Coconut cadang-cadang viroid, 107
Coconut tinangaja viroid, 107
Columnea pale fruit viroid, 108
Cucumber pale fruit viroid, 108
disease management, 109
Grapevine viroid 1B, 108
Grapevine yellow speckle viroid,
108
Hop latent viroid, 108
Hop stunt viroid, 108
infection process, 108
mode of spread, 109
Potato spindle tuber viroid, 108
structure, 107
symptoms, 108
Tomato apical stunt viroid, 108
Tomato planta macho viroid, 108
Viruses
classification, 124-130
control by insecticides, 413-415
control by plant activators, 415
crop diseases, 152-153
definition, 586
desmotubule, 122
disease diagnosis, 152
inclusion bodies, 122, 123
mechanism of infection
attachment to plasmalemma, 119
ingress into host cell, 118, 119
mRNA, 120, 121
protease, 121
reverse transcriptase, 121
self assembly of viral particles,
121, 122
uncoating of virus particles, 119,
120
viral replication, 120, 121
movement protein, 122
multicomponent virus, 112
plasmodesmata, 122
structure
coat proein, 111-118
dsDNA, 113, 116
dsRNA, 116
multipartite viruses, 112
shape, 111-118
size, 111-118
ssDNA, 114, 118
ssRNA, 112-118
symptoms, 140-141
systematic position, 130-140
transmission
aphids, 145, 146
beetles, 148
circulative nonpropagative, 145
circulative propagative, 144
dodder, 152
earwigs, 149
flies, 149
fungi, 151
grafting, 141
grasshoppers, 149
lace bugs, 149
leafhoppers, 146
leafminer, 149
lygacid bugs, 149
mealybugs, 148
mechanical, 141
Viruses, transmission (continued)

mites, 149-150
nematodes, 150
noncirculative nonpersistent, 144
persistent, 144, 145
plant bugs, 149
planthoppers, 147
pollen, 143
protozoa, 151
seed, 141-143
semipersistent, 145
soil, 151
thrips, 147
treehoppers, 149
vegetative propagation materials,
141
water, 151
whiteflies, 147
Virusoids, 586
Vivotoxin, 559, 586
Volunteer plant, 586
Volutin, 12, 13, 586
von Franken, Gottfried, 2
Weibull Model, 207

Western blotting, 586
Wetting agents, 354
Wheat
85
hordecalis), 85
latipons), 85
cyst nematode (Heterodera spp.), 85
chalcoensis), 85
americanum), 85
grass-root gall nematode
(Subanguina radiciola), 86
leaf blotch, 168
list of diseases, 505, 506, 507, 508
87
chitwoodi), 88
naasi), 88
Wheat (continued)
spp.), 87
minyus), 88
thornei), 88
spp.), 88
seed gall nematode (Anguina tritici),
89
spp.), 89
stunt nematode (Merlinius
brevidens), 90
take-all, 255
Wheat Septoria forecasting model
Septoria-Timer, 232
Septoria tritici, 232
Wheat tan spot forecasting model, 231
Xanthomonas arboricola pv. pruni

peach bacterial spot, 15
Xanthomonas axonopodis pv. citri, 14
citrus canker, 14
conditions favoring spread, 219
Xanthomonas axonopodis pv.
malvacearum
cotton bacterial blight, 15
Xanthomonas axonopodis pv. phaseoli

bean common blight, 14
Xanthomonas campestris pv.
campestris
cabbage black rot, 14
cauliflower black rot, 14
Xanthomonas oryzae pv. oryzae
rice bacterial blight, 15, 219
Xanthomonas oryzae pv. oryzicola
conditions favoring spread, 219
rice bacterial leaf streak, 15
Xanthomonas vesicatoria
tomato bacterial spot, 15
Xylella fastidiosa
citrus variegated chlorosis, 14
Yeasts, biocontrol
Aspire, 258
Candida famata, 253
Candida guilliermondii, 253
Candida oleophila, 253
Candida saitoana, 253
Debaryomyces hansenii, 253
Pichia membranefaciens, 253
Torulopsis candida, 253
Zoosporangium, 46, 75
Zoospores, 32
Zygospore, 46

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FOOD PRODUCTS PRESS

P. Vidhyasekaran, PhD, FNA

Food Products Press

Chapter 3. Fungi, Including Chromista and Protozoa

Chapter 4. Parasitic Flagellate Protozoa

Chapter 5. Parasitic Green Algae

Chapter 6. Parasitic Higher Plants

Chapter 7. Parasitic Nematodes

Chapter 8. Phytoplasmas and Spiroplasmas

Chapter 10. Viruses

COMMON DISEASE SYMPTOMS, ASSESSMENT,

Chapter 12. Crop Disease Assessment

Chapter 13. Crop Disease Diagnosis

CROP DISEASE EPIDEMIOLOGY

Chapter 15. Factors Involved in Disease Increase

Environmental Factors Affecting Spread of Bacterial

CROP DISEASE MANAGEMENT

Chapter 18. Biological ControlMycorrhiza

Chapter 19. Breeding for Disease Resistance

Transgenic Plants Expressing Plant Proteinase-Inhibitor

Chapter 22. Chemical ControlBacterial Diseases

Chapter 23. Chemical ControlFungal Diseases

Chapter 24. Chemical ControlFungicide Application

Other Types of Equipment

Chapter 25. Chemical ControlVirus Diseases

Control of Virus Spread by Viricides

Chapter 26. Cultural Methods

Prevention of Introduction of Inoculum

Chapter 27. Exclusion and Eradication

Chapter 28. Plant Activators

HOST DEFENSE MECHANISMS

Chapter 32. Hydroxyproline-Rich Glycoproteins

Chapter 33. Lignin

Chapter 34. Pathogenesis-Related Proteins

Chapter 35. Phenolics

Chapter 36. Phytoalexins

ABOUT THE AUTHOR

P. Vidhyasekaran, PhD, FNA, is the former Director of the Center for

Globally, diseases cause heavy crop losses amounting to several billion

control measures against diseases. He mentioned treating cereal seeds with

MODERN PLANT PATHOLOGY

Corn Stewarts disease (bacterial wilt): Pantoea stewartii (Smith 1898)

The following 12 orders contain 17 families consisting of plant-pathogenic bacteria:

Candidatus is used to categorize prokaryotic entities for which more than

A. tumefaciens (Smith and Townsend 1907) Conn 1942

Corynebacterium were assigned to the genera Curtobacterium,

PantoeaFamily: Enterobacteriaceae; Order: Enterobacteriales; Class:

RathayibacterFamily: Microbacteriaceae; Suborder: Micrococcineae;

X. arboricola pv. pruni (Smith 1903) Vauterin et al. 1995 = X.

X. axonopodis pv. lespedezae (Ayres et al. 1939) Vauterin et al. 1995

X. campestris pv. azadirachtae (Desai et al. 1966) Dye 1978

X. hortorum pv. pelargonii (Brown 1923) Vauterin et al. 1995 = X.

International Committee on Systematic Bacteriology (ICSB) (1992). International

P. parasitica pv. nicotianae causes black shank in tobacco, and P. sojae

hyphae. Entire contents of sporangium are converted into motile zoospores.

Plasmodiophorales contains the family Plasmodiophoraceae, in which two

With a few exceptions, Ascomycota produce their asci in fruiting bodies

Order Diaporthales, Family Valsaceae: Diaporthe, Gnomonia,