NUTRITION AND

DIETETICS
HIGHER SECONDARY - FIRST YEAR

Untouchability is a sin
Untouchability is a crime
Untouchability is inhuman

TAMILNADU
TEXT BOOK CORPORATION
College Road, Chennai - 600 006.
©Government of Tamil Nadu
First Edition – 2004
CHAIRPERSON
Dr. P. Parvathi Easwaran
Former Dean of Home Science
Retd Prof. & Head, Dept of Food Service
Management and Dietetics
Avinashilingam Deemed University, Coimbatore 641043.
REVIEWERS
Mrs Girija Balasundaram Dr. V.Anuradha
S.G.Lecturer Reader Dept of Food Service
Dept of Home Science Management and Dietetics
Queen Mary’ s College Avinashilingam Deemed
Chennai – 600 004 University.
Coimbatore 641043

AUTHORS

Dr. Sheila John Mrs. Sadhana Rajmohan Parimalam

Lecturer S.S. Lecturer, Dept of Home Science
Dept of Home Science Quaid-E-Millat Govt College for
Women’ s Christian College Women, Chennai-600 002
Chennai - 600 006.
Mrs. S. Karthiga Mrs. Anna Rangini Chellappa
Lecturer, Dept of Home Science Lecturer, Dept of Home Science
Quaid-E-Millat Govt Queen Mary’ s College
College for Women Chennai – 600 004
Chennai-600 002
Mrs. B. S. Vasanthi
P.G. Assistant (Home Science)
Presidency Girls Higher Secondary School,
Egmore, Chennai – 600 008
Price: Rs.
This book has been prepared by the Directorate of School
Education on behalf of Government of Tamilnadu.

This book has been printed on 60 G.S.M paper

Printed by offset at:

 PREFACE

This book is the outcome of the apt decision of the

Directorate of School Education, Government of Tamilnadu to
introduce Nutrition and Dietetics as an optional subject at Higher
Secondary level.
People are becoming nutrition conscious. Print and
electronic media pour out nutrition messages to the public. Super
markets are flooded with foods of varied types, natural,
processed, and ready to eat. The common man turns towards
nutrition scientists and dietitians for scientifically proved
information on Nutrition and Dietetics. Hence it is essential that
Nutrition and Dietetics is offered at various levels of education.
This textbook on Nutrition and Dietetics includes content
on Food Science and Nutrition at plus one level, while at plus two
level on Family Meal Management and Dietetics. At plus one
level the student learns the rudimentary aspects while at plus two
level the application aspects are included. The contents are so
arranged that the student gains knowledge with application and
skill.
The authors collected scientifically proved and updated
information from various authentic sources. The reviewers and
chairman offered valuable suggestions on the write up. It is
hoped that the students will understand Nutrition and Dietetics in
the right perspective using this book.
Grateful acknowledgement is expressed to the Director
and Joint Director, School Education, Government of Tamilnadu
for this enriching opportunity.
 1. FOOD GROUPS – A GUIDE IN MENU
PLANNING
Food is the basic necessity of man. It is a mixture of
different nutrients such as carbohydrate, protein, fat, vitamins
and minerals. These nutrients are essential for growth,
development and maintenance of good health throughout life.
They also play a vital role in meeting the special needs of
pregnant and lactating women and patients recovering from
illness.
1.1 FUNCTIONS OF FOOD
Food may be classified according to their functions in
the body.
Functions of Food

___________________________________

Physiological Social Psychological

functions functions functions

________________________________________

Energy yielding Body building foods Protective foods

Foods (Protein) (Vitamins and
(Carbohydrate, minerals)
protein, fat)
Fig1.1 Functions of food
1
Physiological functions of food:
i. Energy yielding foods:
Foods rich in carbohydrates and fats are called energy
yielding foods. They provide energy to sustain the involuntary
processes essential for continuance of life, to carry out various
professional, household and recreational activities and to
convert food ingested into usable nutrients in the body.
The energy needed is supplied by the oxidation of foods
consumed. Cereals, roots and tubers, dried fruits, oils, butter and
ghee are all good sources of energy.
ii. Body building foods:
Foods rich in protein are called body building foods.
Milk, meat, eggs and fish are rich in proteins of high quality.
Pulses and nuts are good sources of protein but the protein is not
of high quality. These foods help to maintain life and promote
growth. They also supply energy.
iii. Protective and Regulatory foods:
Foods rich in protein, minerals and vitamins are known
as protective and regulatory foods. They are essential for health
and regulate activities such as maintenance of body temperature,
muscle contraction, control of water balance, clotting of blood,
removal of waste products from the body and maintaining
heartbeat. Milk, egg, liver, fruits and vegetables are protective
foods.
Social functions of food:
Food has always been the central part of our
community, social, cultural and religious life. It has been an
expression of love, friendship and happiness at religious, social
and family get-togethers.
Psychological functions of food:
In addition to satisfying physical and social needs, foods
also satisfy certain emotional needs of human beings. These
include a sense of security, love and acceptance. For example,
preparation of delicious foods for family members is a token of
love and affection.
2
 Fig.1.2 -Functions of food

3
1.2 ICMR FIVE FOOD GROUPS

TABLE – 1.A
Five Food Group System
Food Group Main Nutrients
I. Cereals, Grains and Products : Energy, protein,
Rice, Wheat, Ragi, Bajra, Maize, Invisible fat Vitamin –
Jowar, Barley, Rice flakes, Wheat B1, Vitamin – B2, Folic
flour. Acid, Iron, Fibre.
II. Pulses and Legumes : Energy, Protein,
Bengal gram, Black gram, Green Invisible fat, Vitamin –
gram, Red gram, Lentil (whole as B1, Vitamin – B2, Folic
well as dhals) Cowpea, Peas, Acid, Calcium, Iron,
Rajmah, Soyabeans, Beans. Fibre.
III. Milk and Meat Products : Protein, Fat, Vitamin –
Milk : B12, Calcium.
Milk, Curd, Skimmed milk,
Cheese
Meat :
Chicken, Liver, Fish, Egg, Meat. Protein, Fat, Vitamin –
B2
IV. Fruits and Vegetables :
Fruits :
Mango, Guava, Tomato Ripe, Carotenoids, Vitamin –
Papaya, Orange. Sweet Lime, C, Fibre.
Watermelon.
Vegetables (Green Leafy) :
Amaranth, Spinach, Drumstick Invisible Fats,
leaves, Coriander leaves, Mustard Carotenoids, Vitamin –
leaves, fenugreek leaves . B2. Folic Acid, Calcium,
Other Vegetables : Iron, Fibre.
Carrots, Brinjal, Ladies fingers,
Capsicum, Beans, Onion,
Drumstick, Cauliflower. Carotenoids, Folic Acid,
Calcium, Fibre
4
 Food Group Main Nutrients
V. Fats and Sugars :
Fats :
Butter, Ghee, Hydrogenated oils, Energy, Fat, Essential
Cooking oils like Groundnut, Fatty Acids
Mustard, Coconut.
Sugars :
Sugar, Jaggery Energy

Source :
Gopalan. C, Rama Sastri B.V. and Balasubramanian
S.C., 1989, Nutritive Value of Indian Foods, National
Institute of Nutrition, ICMR, Hyderabad.

Significance of the five-food group system

The five food group system can be used for the
following purposes :

i. Planning wholesome balanced menus to achieve

nutritional adequacy.
ii. Assessing nutritional status – a brief diet history of an
individual can disclose inadequacies of food and
nutrients from any of the five groups.
Based on the assessment, nutrition education can be
imparted to the individual.

1.3. FOOD PYRAMID :

The food guide pyramid was introduced in 1992 by
USDA (United States Department of Agriculture) as a general
plan of what to eat each day. The food guide pyramid is a
valuable tool for planning a health promoting diet.
By incorporating the principle of balance, variety and
moderation, an individual can still eat their favourite foods
while following the food guide pyramid.

5
 Sugar
and
oil

Milk
and
Meat

Pulses

Fruits and
Vegetables

Cereals

Fig.1.3 -Food guide pyramid

Source:
Srilakshmi .B 2003.Dietetics, New Age International (P)
Publishers Ltd.Chennai.

Balance:
It means choosing food from different food groups.
6
Variety:
This means including different foods within each food
group. For eg. consuming a variety of fruits.
Moderation:
This means keeping serving sizes reasonable. This
involves self control.
The food guide pyramid provides recommendation for
the number of daily servings that should be consumed from each
of the food groups.
The diagram Fig. 1.3, clearly represents that cereals
should form the major bulk of the diet followed by fruits and
vegetables, pulses, milk and meat products and sugars and oil.
The portion size of foods for adolescents (13-18 years) is given
below.
TABLE 1.B
Portion size of foods for adolescents
Number of Portions for
Food Groups Portion Size Adolescents
Girls Boys
Cereals and millets 30 g 10 14
Pulses 30 g 2 2
Milk 100 ml 5 5
Roots and tubers 100 g 1 2
Green leafy 100 g 1 1
Vegetables
Other vegetables 100 g 1 1
Fruits 100 g 1 1
Sugar 5g 6 7
Fats and oils 5g 5 5
(For non-vegetarians substitute one pulse portion with one
portion (50 gm) of egg / meat / chicken / fish.)
Source :
Dietary guidelines for Indians – A manual (1998), National
Institute of Nutrition, ICMR, Hyderabad – 500 007.

7
 Questions
Part- A
Fill in the blanks:
1. Foods rich in carbohydrate and fats are called ______
foods.
2. Foods rich in ________ are called body building foods.
3. Foods rich in protein vitamins and minerals are called
___________ foods.
4. The food guide pyramid is based on the principles of
________ , __________ and ___________.

Part- B
Write short answers:
1. How are foods classified?. Mention the physiological
functions of food.
2. Give the ICMR classification of food groups.
3. List the major nutrients present in fruits and vegetables.
4. Give the portion size of foods for adolescents.
Part- C
Write detailed answers:
1. Explain the functions of food. Enumerate the purpose of
the five-food group system.
2. Give a diagrammatic representation of the food pyramid
and highlight its role as a guide in menu planning.

8
2. COOKING METHODS – MERITS AND
DEMERITS
Food preparation is an important step in meeting the
nutritional needs of the family. Food has to be pleasing in
appearance and taste in order to be consumed. Foods like fruits,
vegetables and nuts can be eaten raw but most foods are cooked
to bring about desirable changes. The process of subjecting
food to the action of heat is termed as cooking.

Objectives of Cooking
1. Cooking sterilizes food: Above 40° C the growth of
bacteria decreases rapidly. Hence food is made safe for
consumption.
2. Cooking softens the connective tissues of meat and the
coarse fibre of cereals, pulses and vegetables so that the
digestive period is shortened and the gastro intestinal
tract is less subjected to irritation.
3. Palatability and food quality is improved by cooking –
Appearance, flavour, texture and taste of food are
enhanced while cooking.
4. Introduces variety – Different dishes can be prepared
with the same ingredients. (Eg.) Rice can be made into
biriyani and kheer.
5. Increases food consumption – Cooking brings about
improvement in texture and flavour thereby increasing
consumption of food.
6. Increases availability of nutrients – Example in raw egg,
avidin binds biotin making it unavailable to the body.
By cooking, avidin gets denatured and biotin is made
available.

9
2.1 COOKING METHODS
Heat is transferred to the food during cooking by
conduction, convection, radiation or microwave energy.
Cooking takes place by moist and dry heat. Moist heat involves
water and steam. Air or fat are used in dry heat.
TABLE – 2A
Cooking methods
Moist Heat Dry Heat Combination
Boiling Roasting Braising
Stewing Grilling
Steaming Toasting
Pressure Cooking Baking
Poaching Sauteeing
Blanching Frying

2.2 MOIST HEAT METHODS

2.2.1 Boiling:
Boiling is a method of cooking foods by just immersing
them in water at 100° C and maintaining the water at that
temperature till the food is tender. Rice, egg, dhal, meat, roots
and tubers are cooked by boiling.
Merits
1. Simple method - It does not require special skill and
equipment.
2. Uniform cooking can be achieved.

Demerits
1. Continuous excessive boiling leads to damage in the
structure and texture of food.
2. Loss of heat labile nutrients such as B and C vitamins if
the water is discarded.
3. Time consuming – Boiling takes more time to cook food
and fuel may be wasted.
4. Loss of colour – water soluble pigments may be lost.

10
2.2.2 Stewing
It refers to the simmering of food in a pan with a tight
fitting lid using small quantities of liquid to cover only half the
food. This is a slow method of cooking. The liquid is brought
to boiling point and the heat is reduced to maintain simmering
temperatures (82°C - 90° C). The food above the liquid is
cooked by the steam generated within the pan. Apple, meat
along with roots, vegetables and legumes are usually stewed.
Merits
1. Loss of nutrients is avoided as water used for cooking is not
discarded.
2. Flavour is retained.
Demerits
1. The process is time consuming and there is wastage of fuel.

2.2.3 Steaming:
It is a method of cooking food in steam generated from
vigorously boiling water in a pan.
The food to be steamed is placed in a container and is
not in direct contact with the water or liquid. Idli, custard and
idiappam are made by steaming. Vegetables can also be
steamed.

Merits
1. Less chance of burning and scorching.
2. Texture of food is better as it becomes light and fluffy.
Eg. Idli.
3. Cooking time is less and fuel wastage is less.
4. Steamed foods like idli and idiappam contain less fat and
are easily digested and are good for children, aged and for
therapeutic diets.
5. Nutrient loss is minimised.
Demerits
1. Steaming equipment is required.
2. This method is limited to the preparation of selected foods.
11
2.2.4 Pressure cooking:
When steam under pressure is used the method is known
as pressure cooking and the equipment used is the pressure
cooker. In this method the temperature of boiling water can be
raised above 100° C. Rice, dhal, meat, roots and tubers are
usually pressure cooked.
Merits
1. Cooking time is less compared to other methods.
2. Nutrient and flavour loss is minimised.
3. Conserves fuel and time as different items can be cooked
at the same time.
4. Less chance for burning and scorching.
5. Constant attention is not necessary.
Demerits
1. The initial investment may not be affordable to
everybody.
2. Knowledge of the usage, care and maintenance of cooker
is required to prevent accidents.
3. Careful watch on the cooking time is required to prevent
over cooking.

2.2.5 Poaching:
This involves cooking in the minimum amount of liquid
at temperatures of 80° C - 85° C that is below the boiling point.
Egg and fish can be poached.
Merits
1. No special equipment is needed.
2. Quick method of cooking and therefore saves fuel.
3. Poached foods are easily digested since no fat is added.
Demerits
1. Poached foods may not appeal to everybody as they are
bland in taste.
2. Food can be scorched if water evaporates due to careless
monitoring.
3. Water soluble nutrients may be leached into the water.
12
2.2.6 Blanching:
In meal preparation, it is often necessary only to peel off
the skin of fruits and vegetables without making them tender.
This can be achieved by blanching. In this method, food is
dipped in boiling water for 5 seconds to 2 minutes depending on
the texture of the food. This helps to remove the skin or peel
without softening food.
Blanching can also be done by pouring enough boiling
water on the food to immerse it for some time or subjecting
foods to boiling temperatures for short periods and then
immediately immersing in cold water. The process causes the
skin to become loose and can be peeled off easily.
Merits
1. Peels can easily be removed to improve digestibility.
2. Destroys enzymes that bring about spoilage.
3. Texture can be maintained while improving the colour
and flavour of food.
Demerits
1. Loss of nutrients if cooking water is discarded.

2.3 DRY HEAT METHODS

2.3.1 Roasting:
In this method food is cooked in a heated metal or frying
pan without covering it. Eg. Groundnut.
Merits
1. Quick method of cooking.
2. It improves the appearance, flavour and texture of the
food.
3. Spices are easily powdered if they are first roasted.
Demerits
1. Food can be scorched due to carelessness.
2. Roasting denatures proteins reducing their availability.
2.3.2 Grilling:
Grilling or broiling refers to the cooking of food by
exposing it to direct heat. In this method food is placed above
13
or in between a red hot surface. Papads, corn, phulkas, chicken
can be prepared by this method.
Merits
1. Enhances flavour, appearance and taste of the product.
2. It requires less time to cook.
3. Minimum fat is used.
Demerits
1. Constant attention is required to prevent charring.
2.3.3 Toasting:
This is a method where food is kept between two heated
elements to facilitate browning on both sides. Bread slices are
cooked by toasting.
Merits
1. Easy and quick method.
2. Flavour improved.
Demerits
1. Special equipment required.
2. Careful monitoring is needed to prevent charring.

2.3.4 Baking:
In this method, the food gets cooked in an oven or oven-
like appliance by dry heat. The temperature range maintained in
an oven is 120°C – 260°C.
The food is usually kept uncovered in a container
greased with a fat coated paper. Bread, cake, biscuits, pastries
and meat are prepared by this method.
Merits
1. Baking lends a unique baked flavour to foods.
2. Foods become light and fluffy – cakes, custards, bread.
3. Certain foods can be prepared only by this method – bread,
cakes.
4. Uniform and bulk cooking can be achieved. Eg. bun,
bread.
5. Flavour and texture are improved.
6. Variety of dishes can be made.
14
Demerits
1. Special equipment like oven is required.
2. Baking skills are necessary to obtain a product with ideal
texture, flavour and colour characteristics.
3. Careful monitoring needed to prevent scorching.

2.3.5 Sauteing:
Sauteing is a method in which food is lightly tossed in
little oil just enough to cover the base of the pan. The pan is
covered with a lid and the flame or intensity of heat is reduced.
The food is allowed to cook till tender in its own steam.
The food is tossed occasionally, or turned with a spatula to
enable all the pieces to come in contact with the oil and get
cooked evenly.
The product obtained by this method is slightly moist
and tender but without any liquid or gravy. Foods cooked by
sauteing are generally vegetables which are used as side dishes
in a menu. Sauteing can be combined with other methods to
produce variety in meals.

Merits
1. Takes less time.
2. Simple technique.
3. Minimum oil is used.
Demerits
1. Constant attention is needed as there is chance of
scorching or burning.

2.3.6 Frying:
In this method, the food to be cooked is brought into
contact with larger amount of hot fat. When food is totally
immersed in hot oil, it is called deep fat frying. Samosa, chips,
pakoda are examples of deep fat fried foods. In shallow fat
frying, only a little fat is used and the food is turned in order that
both sides are browned. Eg. Omlette, cutlets, parathas.
15
Merits
1. Very quick method of cooking.
2. The calorific values of food is increased since fat is used
as the cooking media.
3. Frying lends a delicious flavour and attractive appearance
to foods.
4. Taste and texture are improved.
Demerits
1. Careful monitoring is required as food easily gets charred
when the smoking temperature is not properly maintained.
2. The food may become soggy due to too much oil
absorption.
3. Fried foods are not easily digested.
4. Repeated use of heated oils will have ill effects on health.

2.4 COMBINATION OF COOKING METHODS

Braising :
Braising is a combined method of roasting and stewing
in a pan with a tight fitting lid. Flavourings and seasonings are
added and food is allowed to cook gently. Food preparations
prepared by combination methods are :
Uppuma - Roasting and boiling.
Cutlet - Boiling and deep frying.
Vermicilli payasam - Roasting and simmering.

2.5 MICROWAVE COOKING

Microwaves are electromagnetic waves of radiant energy
with wave lengths in the range of 250 x 106 to 7.5 x 109
Angstroms.
The most commonly used type of microwave generator
is an electronic device called a magnetron which generates
radiant energy of high frequency.
A simple microwave oven consists of a metal cabinet
into which the magnetron is inserted. The cabinet is equipped
with a metal fan that distributes the microwave throughout the
16
cabinet. Food placed in the oven is heated by microwaves from
all directions.

Fig. 2. 1- Microwave oven

1.Door release button 2. See –through window 3. Door
safety lock system 4.External air vents 5.Control panel
6.Identification plate 7.Glass tray 8.Roller ring
Source: Srilakshmi B.(2003) Food Science , New Age
International (P) Publishers Limited.Chennai.
Moist foods and liquid foods can be rapidly heated in
such ovens. Food should be kept in containers made of plastic,
glass or china ware which do not contain metallic substances.
These containers are used because they transmit the microwaves
but do not absorb or reflect them.
Merits
1. Quick method – 10 times faster than conventional method.
So loss of nutrients can be minimised.
2. Only the food gets heated and the oven does not get
heated.
3. Food gets cooked uniformly.
17
 4. Leftovers can be reheated without changing the flavour
and texture of the product.
5. Microwave cooking enhances the flavour of food because
it cooks quickly with little or no water.
Demerits
1. Baked products do not get a brown surface.
2. Microwave cooking cannot be used for simmering,
stewing or deep frying.
3. Flavour of all ingredients do not blend well as the cooking
time is too short.
2.6 SOLAR COOKING
Solar cooking is a very simple technique that makes use
of sunlight or solar energy which is a non-conventional source
of energy.
Solar cooker consists of a well insulated box which is
painted black on the inside and covered with one or more
transparent covers.
The purpose of these transparent covers is to trap heat
inside the solar cooker. These covers allow the radiation from
the sun to come inside the box but do not allow the heat from
the hot black absorbing plate to come out of the box. Because
of this, temperature upto 140°C can be obtained which is
adequate for cooking.
Merits
1. Simple technique – requires no special skill.
2. Cost effective as natural sunlight is the form of energy.
3. Original flavour of food is retained.
4. There is no danger of scorching or burning.
5. Loss of nutrients is minimum as only little amounts of
water is used in cooking.
Demerits
1. Special equipment is needed.
2. Slow cooking process.
3. Cannot be used in the absence of sunlight – rainy season,
late evening and night.
18
 Fig. 2.2-Solar cooker

1.Solar plane mirror 2.Cooking container3.Glass sheet

4. Cover 5. Insulation material glass 6.Outer box
7. Handle 8.Mirror support 9.Hinged adjuster and guide

Source: Srilakshmi B.(2003) Food Science , New Age

International (P) Publishers Limited.Chennai.

19
 QUESTIONS

Part- A
Fill in the blanks:
1. ________ is a method of cooking foods by just immersing
then in water at 100º C
2. Simmering of food in a pan with a tight fitting lid using small
quantities of liquid to cover only half the food is known as
___________
3. When steam under pressure is used the method is known as
_________
4. ___________ is a method use to prepare cakes.
5. When food is totally immersed in hot oil, it is called
_________.
6. ________ is a combined method of roasting and stewing in a
pan with a tight fitting lid
Part- B
Write short answers
1. What are the objectives of cooking food?
2. Bring out the differences between stewing, steaming and
sauteing.
3. What is the best method of preparing rice and dhal? Justify
your choice of cooking method.
4. Write a note on solar cooking and its merits and demerits
Part- C
Write detailed answers
1. Compare the various moist heat methods of cooking.
Highlight the merits and demerits of each type.
2. Give a brief account about the principles, merits and
demerits of microwave cooking.
3. Explain the cooking method employed in the preparation of
(a) Bread, (b) Idli, (c) Chapathis
4. Discuss the dry heat methods of cooking?

20
 3. CEREAL AND CEREAL
PRODUCTS
Cereals form the staple food of the human race. In India
wheat, rice, maize (corn), oats, jowar, ragi and bajra are the
common cereals and millets used.

3.1 STRUCTURE OF RICE AND WHEAT

The overall structure of all cereal grains is basically
similar. Rice grains resemble wheat but is smaller than that of
wheat. It is flattened laterally and has no ventral furrow.
Wheat cereal grains are composed of an outer bran coat,
a germ and a starchy endosperm.

rice wheat
a. Bran b. Endosperm c. Germ
Fig. 3.1 Structural Parts of rice and wheat
Source: Sumati Mudambi, R and Shalini, M. Rao 1989 Food
Science. New Age International (P) Publishers Ltd, Chennai.
21
 The longitudinal structure of wheat grain depicting the various
layers is represented diagrammatically in fig.3.2

Fig. 3.2 Longitudinal structure of wheat grain

Source: Srilakshmi B. 2003 Food Science. New Age
International (P) Publishers Ltd Chennai.
Bran:
Bran is the outer layer of the kernel and constitutes 5
percent of the kernel. During milling the bran is discarded. Bran
is rich in fibre and minerals. It is also a good source of thiamine
and riboflavin.
Aleurone Layer:
This is located just under the bran, which is rich in
protein, phosphorus and thiamin and contains moderate amounts
22
of fat. The aleurone layer makes up about 8 percent of the
whole kernel. This layer is lost in the milling process along with
bran.
Endosperm:
This is the large central part of the kernel and constitutes
84 – 85 percent of the kernel. The endosperm cell consists
mainly of starch and protein and little mineral matter and fibre
and only half a trace of fat. The vitamin content of the
endosperm is low.
Germ :
This is a small structure at the lower end of the kernel
and is separated from the endosperm by the scutellum. It makes
up 2 – 3 percent of the whole kernel. It is rich in protein, fat,
vitamins and minerals.
The germ serves as a store of nutrients for the seed when
it germinates. During milling some of the germ is lost along
with the bran and aleurone layer.

3.2 NUTRITIVE VALUE OF CEREALS

Cereals are an important and economic source of energy.
Hundred grams of cereals supply 340 kilo calories of energy.
Cereals are also a significant source of proteins (8 – 11 percent)
in the diets of people whose staple food is cereals.
However, cereal protein is incomplete as it lacks an
essential amino acid, lysine. This lack is made up when cereals
are eaten along with other protein foods such as dhals, pulses
and milk.
Wheat flour contains glutelin and gliadin as proteins
which are commonly known as gluten. The strength of the
wheat flour is based on the quality of gluten used.
Whole grains chiefly furnish starch, proteins,
minerals, B -Vitamins and fibre.
Refined cereals lose part of the protein, minerals, and B -
Complex vitamins in milling. They contain a little more starch
than whole cereals.
23
 Whole grains contain more vitamins, minerals and fibre
than refined grain and are valuable dietary sources of iron,
phosphorus, thiamine and fibre.

3.3 PARBOILING AND MILLING – EFFECT ON

NUTRIENT CONTENT
Parboiling is a process of soaking paddy in water at 65° -
70° C for 3 – 4 hours. The water is drained and the soaked
paddy is steamed in the same vessel for 5 to 10 minutes. The
paddy is dried in the sun or mechanically dried.
Advantages of Parboiling:
1. Dehusking of parboiled rice is easy.
2. Grains become tougher resulting in reduced losses
during milling.
3. Part of the scutellum and germ which are rich in B –
Vitamins get fixed to the grain and hence loss of B –
Vitamins are less. The retention of thiamine, riboflavin,
niacin and folic acid in parboiled rice is greater than that
of polished or hand pounded rice.
4. It improves digestibility.
5. It swells more when cooked to desired softness.
Milling:
Milling is the process, which removes the coarse outer
layer of bran and germ. Paddy is milled by hand pounding or
mechanical rice millers.
The process of milling involves the following steps.
• Rice is passed through two stone or rubber discs rotating
at different speeds and by shearing action on the grain,
the hull is pulled away. The whole kernel from which the
hulls have been removed is known as brown rice.
• This is then milled in a machine called pearler to remove
coarse outer layers of bran and germ by a process of
rubbing, resulting in unpolished milled rice. Some
amount of breakage of rice occurs in this milling.
24
 • Unpolished rice is liable to develop rancidity and so it is
polished in a brush machine which removes the aleurone
layer and yields “polished rice”.
• Sometimes the polished rice is further treated in a device
known as trumbol to give a coating of sugar and talc to
produce a brighter shine on the grains.
• Rice is separated from the broken kernels. Large kernels
are called second heads, medium ones are called
screenings, smallest ones are called the brewers rice.
The percentage loss of different nutrients during milling
are
protein 15%
fat 82%
thiamine 85%
riboflavin 70%
pyridoxine (Vitamin B6) 50%.
The degree of milling determines the amount of nutrients
removed.
Losses during milling can be compensated by the following
processes.
• By under-milling or unpolishing rice the loss of nutrients
can be reduced.
• A second method is that of increasing vitamin retention
by processing the rough rice prior to milling. This is
done by parboiling which is commercially known as
converted rice.
• Another means of remedying the losses occurring in the
milling of rice is the artificial enrichment of the grain.
A premix has been developed in which the rice is wetted
with a solution of thiamin and niacin, then dried. A
second coating of iron pyro phosphate is distributed on
the rice.
The rice premix is highly resistant to washing, cooking
and storage losses.
25
 (a) unmilled raw rice (b) Milled raw rice

(c) unmilled parboiled rice (d) milled parboiled rice

Fig. 3.3 – Effect of milling of raw and parboiled rice
(a) Unmilled raw rice - Germ and pericarp are intact in raw
rice, Vitamin B1 and other vitamins are concentrated
mainly in the germ and pericarp. When these are
removed by milling, the grain has lost most of its
vitamins.
(b) Milled raw rice – Germ and pericarp are removed
(c) Unmilled parboiled rice- The vitamin has diffused
through the endosperm
26
 (d) Milled parboiled rice : Although germ and pericarp have
been removed, the grains still contains most of the
vitamins
3.4 NUTRIENT CONTENT OF RAGI, MAIZE, AND
JOWAR
Ragi :
Ragi or finger millet is widely consumed without any
refining by many people in rural areas. It contains B Vitamins
but is poor in thiamine. Ragi is rich in minerals especially
calcium. It is also rich in fibre and is a fair source of iron.
Maize or corn :
Maize, like any other cereal is rich in calories. It is
deficient in amino acid lysine. It is a good source of carotene
and contains thiamine and folic acid in appreciable amounts.
Jowar :
Jowar or Sorghum is grown in Maharashtra, Karnataka,
Madhya Pradesh, Gujarat, Uttara Pradesh and parts of Tamil
Nadu. It is rich in carbohydrate, and B – Complex vitamins. It
is poor in vitamin – A and rich in dietary fibre. Compared to
rice, jowar is richer in protein but the quality is not as good as
rice protein.
3.5 MALTING OF CEREALS
Malting is a controlled germination process, which
activates the enzymes of the resting grain resulting in the
conversion of cereal proteins and other macromolecules.
Generally barley is the grain used in the production of malt.
Other grains used in the preparation of malt include wheat,
jowar and ragi.
The process of malting of cereal grain consists of the
following steps :
1. Selection of grain and cleaning.
2. Steeping in cold water for 36 hours with 2 to 3 changes
in water.
27
 3. Germination : The grains are spread on wire mesh trays
and kept for 3 days. Water is sprinkled over each of
these trays. Amylases and proteases are formed.
4. Kilning : The germinated grains are dried at slow rate on
kilns. The amylases act on starch hydrolysing it to
dextrin and proteases act on protein hydrolysing them to
peptones.
Amylase Rich Food (ARF) is germinated cereal flours
which are extremely rich in the enzyme alpha –
amylase. ARF are excellent weaning foods because
they reduce the bulk of weaning foods and are energy
dense.
5. Malt is used in pharmaceutical preparations, breakfast
cereals, malted milk confectionaries, infant foods, bakery
products, candies and in brewing.

3.6 PROCESSED CEREAL PRODUCTS

Products of wheat
Whole wheat flour:
It is obtained by grinding whole wheat. It contains the
finely ground bran, germ and endosperm of the whole kernel.
Maida:
It is refined wheat flour. The bran and germ are
separated in making white flour or maida. Maida bakes more
uniformly into a loaf of a greater volume and it is more bland in
taste and more easily digested. It is used in the manufacture of
macaroni products.
Semolina:
It is coarsely ground endosperm and its chemical
composition is similar to that of white flour.
Macaroni Products:
These products include macaroni, spaghetti, vermicelli and
noodles.

28
 The starchy endosperm of wheat is coarsely ground into
semolina which is made with water into a thick dough. The
dough is placed in a cylinder, the lower end of which is fitted
with a disc perforated with openings. As the dough is forced
through the openings various shapes are formed. Macaroni is a
tube form, spaghetti may be either tube or rod, vermicelli is a
tiny rod and noodles are flat strips.
Malted Wheat:
The process of malting helps in the production of malted
cereal flour Malted cereal flour is inexpensive and can be made
at home as well as commercially.
Malt is used in brewing and in the preparation of malt
extract for pharmaceutical purposes and in the preparation of
malted milk powder.
Broken Wheat:
Broken Wheat is whole wheat coarsely ground into large
particles. As the losses during milling is little, it is a very
nutritious food.

Rice products:
Rice bran:
Breakage of the white rice kernel during milling results
in small fragments of the endosperm becoming part of the bran
fraction. Parboiled rice bran is normally finely granulated and
light tan in colour. It has a bland flavour and can be used in the
preparation of bread, snacks, cookies and biscuits. In addition,
rice bran is a very rich source of dietary fibre. It is therefore an
effective stool bulking agent.
Rice bran oil :
Rice bran oil is obtained by extracting edible grade oil
from rice bran. The National Institute of Nutrition, (NIN)
Hyderabad has certified that this oil is toxicologically safe for
human consumption. This oil is rich in Vitamin E. In addition, it
has cholesterol lowering effect than other oils.
29
Puffed Rice:
Puffed rice is obtained when sun ripe paddy is filled in
earthen jars and is moistened with hot water. After 2-3 minutes
the water is decanted and the jars are then kept in an inverted
position for 8-10 hours.
The paddy is exposed to sun for a short time and then
parched in hot sand at 190 -210º C for 40-45 seconds. During
parching, the grains swell and burst into soft white products.
The parched grains are sieved to remove sand and winnowed to
separate the husk.
Rice flakes:
Rice flakes are made after soaking the paddy in hot
water, parching it by roasting and then flattening it by force
while it is hot to form flakes. It retains a large part of iron and
B-vitamin of the aleurone layer. The roasting helps to toast the
grain, resulting in partial cooking of the grain. It needs very little
time to prepare and is used as a snack. It should be free from
bran, broken particles, fragments of the seed coat, insects,
stones, trash and bad odour.

Products of Maize
Corn oil:
Corn oil is extracted from corn germ. Corn or maize oil
is a highly desired vegetable oil owing to its relatively high level
of linolenic fatty acid and its excellent flavour.
Popcorn:
Popcorn is prepared by heating the kernels of corn.
During heating, the water vapour within them expands,
increasing the pressure until it is sufficient to make the kernels
explode or “pop”. It is used as a snack for children. Popping can
be done with or without fat.
Corn starch:
It is made by a process of wet milling in which the hull
and germ are removed. The corn is then ground and mixed with
water. The semi liquid material is separated by passing it over
30
sieves or centrifuging it. The starch settles out while most of the
protein remains suspended.
The starch is then washed, dried and powdered. Corn
starch is widely used because it is inexpensive, lacks
characteristic flavour and cooks to a smooth and almost clear
paste in water or other clear liquid and superior to wheat flour or
potato starch.
3.7 FERMENTED CEREAL PRODUCTS
The term fermentation refers to the breakdown of
carbohydrate like matter under either aerobic or anaerobic
conditions. The organisms involved may be bacteria or moulds.
During fermentation microorganisms produce gas and help in
leavening the batter or dough. They also produce flavouring
substances.
Advantages of Fermentation:
1. Flavour and texture of the product is improved.
2. Vitamin B and C content is increased.
3. The product is easily digestible.
4. Acid by-products formed during fermentation inhibits
the growth of harmful microorganisms.
5. Variety in the diet:- Traditional Indian recipes like idli,
dosa, appam, dhokla and rice vadam are cereal based
fermented products.
Idli :
Idli is a fermented and steamed food prepared using
parboiled rice and black gram dhal in proportion of 3 : 1. The
ingredients are ground separately, mixed together and allowed to
ferment overnight. Fermentation is facilitated by bacteria such
as Lactobacillus lactis, Streptococcus lactis and Leuconostoc
mesenteroides. The mucilagenious material in black gram dhal
helps in the retention of carbon- dioxide during fermentation.
Dosai :
This is prepared from a fermented batter of rice and
black gram (4:1). Ingredients are ground fine and salt is added
and fermented overnight.
31
Dhokla :
It is prepared using rice and bengalgram dhal. The
ingredients are soaked, ground coarsely and fermented
overnight. The batter is steamed in a pie-dish and cut into
desired shapes and garnished.
Appam:
It is a rice batter fermented with yeast and coconut milk
or coconut water and cooked in a special appam kadai by
covering with a dome shaped lid.
Rice vadam :
This is prepared by using left over cooked rice, which is
soaked in excess water and allowed to ferment overnight. The
water is drained and rice is mashed. Finely cut onions, chillies
and lime are added and it is made into small vadam and sun-
dried.
Bread :
Flour, water, milk, salt, sugar, butter and yeast are the
ingredients used in the preparation of bread. All the ingredients
are mixed and allowed to rise.
Fermentation is effected by the action of yeast enzyme
zymase on the glucose in the dough producing alcohol and
carbon dioxide. The fermented dough is kneaded by hand
(knock-back) to remove gas, redistribute yeast cells, subdivide
gas cells and increase the uniformity in size.
The fermented dough is then sized, shaped and placed in
pans for proofing at 38° C - 48° C for 45 – 60 minutes and
baked for 30 minutes at 204° C – 232° C

3.8 ADVANTAGES OF INCLUDING A COMBINATION

OF CEREALS IN THE MENU
Cereals are the main source of energy in Indian diets
contributing 70 – 80 percent of daily energy intake of majority
of Indians. The major cereals consumed in India are rice, wheat,
jowar, bajra and ragi.
32
 Rice is the staple diet of South Indians. However, rice
among the cereals is a poor source of calcium and iron. Whole
wheat is a fair source of protein and fibre.
Although rice contains less protein when compared to
other cereals, its protein quality is better than that of other
cereals.
Ragi is rich in minerals especially calcium. Millets
including ragi are rich in minerals and fibre. Inclusion of millets
will help in making up deficiencies of some minerals in the diet
besides providing bulk to the diet, particularly rice based ones.
The nutritive value of cereals varies with the part of the
grain used. All whole cereals furnish starch, protein, iron,
phosphorus, thiamin and fibre but refined cereals lose part of
these nutrients during the milling process.
A judicious combination of different cereals in the days
diet will help to meet the nutrient requirements.
For example wheat dosai, rice flakes payasam, ragi adai
and broken wheat uppuma can be included in the menu instead
of rice-based meals alone. Batters used for idli and dosai and
doughs used for chappathis can be prepared using a combination
of cereal flours. This will contribute different nutrients to the
days diet.

3.9 ROLE OF CEREALS IN COOKERY

• Cereals form the staple diet and contribute to most of the
calorie requirement and half of the protein requirement.
Cereals improve the quality of pulse protein. They are
excellent source of starch and B vitamins. Cereals also
contribute to satiety and are used to prepare the main
dish. No meal can be made without cereals.
• Cereals are used as thickening agent, e.g. corn flour in
custards, corn flour in white sauce, macaroni in soups.
• Cereals are used as coating agent, e.g., maida paste in
cutlets or bread crumbs in cutlets.

33
 • Cereals are used in sweet preparations, e.g., rice,
payasam, wheat halwa.
• Malted cereals are used in the preparation of beverages
and weaning foods.
• Cereals products like corn flakes and rice flakes are
used as ready to use foods.
• Fermented foods made from cereals are used as breakfast
foods or snacks, e.g., idli, dhokla.

QUESTIONS

Part-A
Fill in the blanks:
1) ________________ is the outer layer of the kernel.
2) Cereals are deficient in amino acid _______________.
3) Parboiling reduces the loss of vitamins _____________.
4) The process of removing bran and germ is called
____________.
5) _____________ is a controlled germination process which
activates the enzymes of the resting grain.

Part-B
Write short answers
1) Define parboiling. Highlight its advantages.
2) What are the advantages of fermentation?
3) Discuss the advantages of including a combination of
cereals in the menu.
4) Explain the roles of cereals in cookery?

34
 Part-C

Write detailed answers

1) Discuss the nutrient content and structure of cereals.

2) Compare the nutritional significance of rice and ragi.
3) What is fermentation? What are the cereal products
prepared by fermentation?
4) How is malting done? Explain the process.
5) Discuss the role of cereals in cookery. Highlight the
advantage of including combination of cereals in the
days menu.
6) Give a detailed account on the different types of
processed cereal products?
7) How is milling done? How can nutrient losses be
prevented in milling?

35
 4. PULSES
Pulses are the edible fruits or seeds of pod-bearing
leguminous plants. The term pulse in India is used for edible
legumes and dhal is used for decuticled split legumes.
Bengal gram, red gram, black gram, green gram, lentil,
horse gram, peas and kesari dhal are some of the major pulse
crops in India. Soyabean is also grown.

4.1 NUTRIENT CONTENT OF PULSES

Pulses give 340 calories per 100 gm which is almost
similar to cereal calorie. They are a rich source of protein
containing about 18 – 25 percent protein. Soyabean is an
exception containing about 35 to 40 percent protein.
All pulses contain sufficient amount lysine which is
deficient in cereals and therefore they can supplement cereal
protein. A mixture of cereals and pulses is superior to that of
either one. Hence a combination of cereals and pulses is ideal
for human consumption.
Pulses contain 55 – 60 percent of carbohydrate including
starch soluble sugar and fibre.
They contain 1.5 percent lipids. Pulses also contain
calcium, magnesium, zinc, iron, potassium and phosphorus.
They are a poor source of carotene and vitamin C but
fairly rich in niacin. Germination increases the vitamin C
content of pulses. The thiamine content of pulses is equal to or
exceeds that of cereals. Being rich in B - Vitamins, they
contribute significantly to B – Vitamin intake.
4.2 TOXIC SUBSTANCES IN PULSES

Some toxic substances are naturally present in some pulses.

These include trypsin inhibitors and haemagglutinins. Trypsin
36
inhibitor, as the name indicates, interferes with digestion of
proteins by inhibiting the action of the enzyme trypsin.
Haemagglutinins combine with haeme and thus destroy
haemoglobin. Fortunately, both of these toxic substances are
destroyed by heat, which is used in the normal cooking process.
Broad beans contain some toxic substances. When these
beans are consumed raw a disease called favism occurs. This
disease is characterized by haemolytic anemia. Since human
beings usually do not consume broad beans raw, they are not
likely to suffer from favism.
Kesari dhal also contains a toxic substance. This dhal is
grown in Madya Pradesh(M.P) It was observed that during the
drought conditions, only this dhal is grown and used as a staple
food. When this dhal is consumed over a long time paralysis of
lower limbs occurs in males. This is known as lathyrism.
It is reported that when the intake of kesari dhal is restricted to
30 per cent of the total calorie intake, no adverse effects are
observed. Therefore it is important to ensure that the intake of
this dhal must be restricted to a maximum of 30 percent of the
total calorie intake.

4.3 GERMINATION – NUTRIENT ENHANCEMENT

Germination is a process that involves the soaking of
pulses overnight. The water is then drained and the seeds are
tied in a loosely woven cotton bag and hung.
Water is sprinkled twice or thrice a day and sprouts
usually appear within 6 – 8 hours.
Advantages of germination :
1. Vitamin C is synthesised during germination. The
increase in vitamin C is around 7 – 20 mg per 100 gm of
pulses.
2. Riboflavin, niacin, choline and biotin are increased.
3. Starch is converted into sugars.
4. It reduces the anti-nutritional and toxic factor in pulses.

37
 5. Increased variety in the diet as sprouted pulses can be
added to salads.
6. Dormant enzymes get activated and digestibility and
availability of nutrients is improved.
7. Minerals like calcium, zinc and iron are released from
bound form.
8. Sprouted pulses can be eaten raw, since germination
improves taste and texture.
4.4 FACTORS AFFECTING PULSE COOKERY
1. Soaking in water hastens the cooking of dried pulses.
2. Soaking in boiling water reduces cooking time as the
enzyme phytase present in the legume is inactivated.
3. Hard water prolongs the cooking time of dried pulses.
This may be due to the reaction of calcium and
magnesium ions in hard water with the pectic
constituents of dried beans.
4. Addition of cooking soda (Sodium bicarbonate) will
hasten cooking. However this causes loss of thiamine.
Excess soda also makes cooked legumes dark and
mushy.
5. Addition of acidic component such as tomato juice
tamarind juice prolong the time required to make pulses
tender.
6. Cooking time is considerably reduced when pulses are
cooked by the use of steam under pressure.
4.5 SOYA PRODUCTS AVAILABLE IN THE
MARKET AND THEIR USE
Soyabean with its high protein content is considered as a
substitute for meat protein which is expensive.
Soyabean can be processed to obtain the following food
products.
1. SOYA FLOUR:
Soyabean is slightly roasted and ground to yield flour.
Oil is sometimes removed from the bean to give defatted soya
38
flour which has a higher keeping quality. Soya flour is used in
combination with wheat flour in the preparation of chapathis. It
can also be incorporated in the batter used in the preparation of
bajji, vadai and pakoda.
2. SOYA MILK :
The milk is prepared by grinding soaked beans with
water. It is then passed through a mill in a stream of water. The
emulsion that is obtained is filtered and transferred to a boiler
and mixed with vitaminised margarine to which sugar, salt,
calcium and malt are added. The mixture is cooked for 20
minutes emulsified and then dried. The white emulsion thus
expressed from soyabean has the appearance of milk.
3. SOYABEAN CURD :
Tofu or soyabean curd is prepared from soya milk. The
curd is precipitated from milk emulsion by adding calcium
sulphate. It is allowed to settle, and then it is separated washed
and dried. It is a soft delicate structure which can be cut into
pieces. It can be used like paneer in various preparations.
4. TEXTURED VEGETABLE PROTEIN (TVP) :
Textured Vegetable Protein is prepared using defatted
soya flour from which most of the oil and carbohydrates are
removed. The flour contains 70 percent protein and is made into
dough to which colour and flavour are added. It is at times
fortified with vitamins.
The dough is extruded through equipment at high
temperature and pressure and the product is expanded by sudden
release of pressure.
The extruded granules are marketed as TVP. It is
rehydrated with water before use in the preparation of various
vegetarian and non-vegetarian dishes.
5. SOYA PROTEIN ISOLATES
Soya protein isolates are protein granules, isolated by
processing, It is fortified with vitamins and minerals and used
as a complementary food.
39
 Apart from these fermented soya products, soya sauce
and soya paste are used in the preparation of chinese dishes.

4.6 ROLE OF PULSES IN COOKERY

• Pulses are rich in protein and B vitamins and improve
the quality of cereal protein.
• Pulses give satiety due to high protein and fibre content.
• Pulses improve flavour and consistency of dhal sambar
and rasam.
• They contribute to fermentation in preparation of idli and
dosa.
• They are used in snacks like sundal, bajji, panipuri and
bhelpuri.
• They are used in salads, e.g., sprouted gram.
• They are used in desserts like paruppu payasam and
sweets like mysore pak and laddu.
• They are used as thickening agent e.g., Bengal gram
flour in gravies.
• Roasted pulses are used in making chutneys and chutney
powders.
• They are used as part of seasonings in curries.

Questions

Part-A
Fill in the blanks:
1) Pulses give _________ calories per 100gms.
2). Soyabean contains about _________ percent protein.
3). Pulse protein are deficient in _______ amino acids.
4). All pulses contain sufficient amount of _______which is
deficient in cereals.
5). Germination increases the vitamin _________ content of
pulses.

40
1) ___________ is a process that involves the soaking of
pulses overnight.
2) Addition of cooking soda causes loss of _________in pulses.
3) ___________ is prepared using defatted soya flour from
which most of the oil and carbohydrates is removed.

Part-B
Write short answers
1). Discuss the nutrient content of pulses.
2). Justify the nutritional significance of cereal – pulse
combinations.
3). Discuss the role of pulses in cookery.
4). Write a short note on toxic substances in pulses

Part- C
Write detailed answers
1). What is germination? Explain the effect of germination on
the nutrient content pulses?
2). Discuss the factors affecting the cooking quality of pulses.
3). List the products prepared from soyabean and highlight its
use in Indian cookery.

41
 5. VEGETABLES AND FRUITS

India with its diverse, but favourable agroclimatic conditions

produces a wide range of tropical and temperate fruits and
vegetables. The annual production of these crops is about 53
million tonnes.
Vegetables are plants or parts of plants served with the
main course of a meal.
Apart from the nutritive value, vegetables probably do
more than any other group of foods to add appetising colour,
texture and flavour to our daily food.
With the wide choice of colour of vegetables, it is
possible to select a vegetable with a desired colour to highten
the appearance of a meal.
The texture of a vegetable varies depending upon
whether it is served raw are cooked. The texture and
appearance of meals can then be varied by the way the
vegetable is served.
Vegetables contain a wide range of characteristic
flavours. By a proper choice of vegetables, the desired flavour
of a meal can be obtained.
A fruit is the edible and juicy product of a tree or plant
and consists of the matured ovary including its seeds and
adjacent parts. Usually fruits are sweet, with a wide range of
flavours, colours and textures.

5.1 CLASSIFICATION OF VEGETABLES

Vegetables can be classified into three groups according
to their nutritive value.
Ø green leafy vegetables
Ø roots and tubers
Ø other vegetables

42
 VEGETABLES

________________________________________

Green leafy vegetables Roots and tubers Other

vegetables
(Eg. Agathi, amaranth, (Eg. Potato, (Eg. Brinjal,
drumstick leaves, beetroot, onion, bitter gourd
cabbage, spinach, Carrot, yam, ladies finger,
fenugreek leaves,) tapioca) cauliflower.)

Fig.5.1 Classification of vegetables

5.2 NUTRIENT CONTENT OF VEGETABLES AND

FRUITS
VEGETABLES
i. Green Leafy Vegetables :
They are an inexpensive rich source of many nutrients
such as – carotene, ascorbic acid, folic acid, calcium,
iron and fibre. They are a poor source of protein.
ii. Roots and Tubers :
Roots and tubers are rich in carbohydrates and are a
source of energy in the diet. Carrot and yellow varieties
of yam are rich in carotene and potato contains Vitamin
C. Tapioca and yam are rich in calcium. Roots and
tubers are a poor source of iron, protein and a fair source
of B – Vitamins.
iii. Other Vegetables :
These are a good source of dietary fibre and add variety
to the diet. They are a fairly good source of vitamins and
minerals. (Eg.) brinjal, ladies finger, cauliflower,
cucumber, gourd varieties.
43
FRUITS
A fruit is a mature ovary of a flower. The fleshy portion
of the pericarp makes up the chief edible portion of the
fruit.
Fruits can be classified as follows :
Berries - Gooseberry, grapes, strawberry.
Citrus - Lemon, lime, orange, sweet lime.
Drupes - Peach, plums, apricot.
Melons - Water melon, musk melon
Pomes - Apple, pear.
BERRIES :
Berries are fruits with layers of pericarp(fruit coat)
which are often homogenous, except for the skin on the outside.
The pericarp layers are pulpous and juicy, and contain seeds
embedded in the pulp mass. The fruits have fragile cell structure
that is damaged by rough handling or freezing.
CITRUS FRUITS:
These fruits belong to the genus Citrus which contains
about 16 species of evergreen aromatic shrubs and trees mostly
with thorny branches distributed throughout the tropical and
subtropical regions of the world. The common citrus fruits are
orange, lemon and lime. The bright colour, pleasing flavour and
sweetness make them a favourite fruit. They are served as juice
and can be eaten raw.
DRUPES:
Drupes are edible fruits with a thin skin, and juicy flesh
enclosing a single seed (Stone). Apricots, cherries, peaches and
plums belong to this group.
MELONS:
Melons belong to the same family as cucumbers
(Cucurbitaceae). Melons are commonly eaten raw. Their flesh
consists of about 94% water and only 5% sugars. The seeds
stripped of their hard coats may be eaten and also yield an
edible oil.
44
POMES
Pomes are fruits of apple and pear trees. The receptacle,
surrounds the ovaries in the flower, enlarges to become edible
and juicy, and encloses the cells containing the seeds.
Fruits particularly citrus varieties and guava are a good
source of vitamin C. Yellow fruits like mango and papaya
contain – carotene. Banana is a good source of carbohydrate
and hence energy. Fruits are a poor source of protein and fat
with the exception of avacado.
Fruits also contain fibre and minerals such as sodium,
potassium and magnesium. They are not a good source of
calcium. Dry fruits, seethaphal and watermelon contribute
appreciable amounts of iron.
5.3 PIGMENTS AND FLAVOUR COMPOUNDS
Chlorophyll :
Chlorophyll is the green pigment of leafy vegetables and
other green coloured vegetables
Carotenoids:
Carotenoids are the yellow, orange, red fat soluble
pigments distributed in nature. They are divided into three
groups viz. carotenes present in carrot, green leafy vegetables
and other fruits, lycopenes present in tomatoes and xanthophylls
present in yellow fruits.
Pigments that contain the phenolic group include
anthocyanin, anthoxanthin, leucoanthoxanthin, catechin,
quinones and betalins. The first four groups are collectively
known as “Flavanoids”
Anthocyanin :
They are a group of reddish water-soluble pigments
occurring in many fruits and vegetables. Cherries, red apples,
pomegranates have their colour appeal due to anthocyanins.
Anthoxanthins :
They are colourless white to yellow pigments that give
colour to cauliflower, onions, spinach or other leafy vegetables.
In green leafy vegetables the colour is masked by chlorophyll.
45
Leucoanthoxanthins :
They are colourless and contribute to the puckeriness or
astringency of some foods, such as apple and olives. They also
play an important role in the enzymatic browning of fruits.
Catechins :
They are pigments that are involved in enzymatic
browning.
Betalins :
They are the red water soluble pigments found in
beetroot and berries.
Quinone :
The yellow pigment juglone is a quinone present in
walnut.
Mangiferin :
This is the yellow pigment belonging to the xanthone
group. It is found in mangoes.

Tannins :
They are complex mixtures of polymeric polyphenols.
The appearance of tannins ranges from colourless to yellow or
brown. Tannins contribute to the astringency of foods and also
to enzymatic browning.
Flavour Compounds :
The flavour of fruits and vegetables are extremely
important to their acceptance in the diet.
The overall flavour impression is the result of the tastes
perceived by the taste buds in the mouth and the aromatic
compounds detected by the epithelium in the olfactory organ in
the nose.
In fruits and vegetables, this means that sugars, acids,
salts and bitter quinine-like compounds are tasted while the food
is chewed in the mouth.
Sweetness may result from the presence of glucose,
galactose, fructose, ribose, arabinose and xylose.

46
 All fruits and vegetables contain a small amount of salt,
which is detected in the overall taste impressions contributing to
flavour.
The natural flavours of vegetables are due to mixtures of
aldehydes, alcohol, ketones, organic acids and sulphur
compounds. Some fruits and vegetables have an astringent taste
attributed to phenolic compounds or tannins.
Two types of vegetables viz., vegetables belonging to the
Allium and Cruciferae families have strong flavours resulting
from the presence of various sulphur containing compounds.
Allium is the genus that includes onions and garlic. Members of
the family cruciferae, which include broccoli, cabbage, turnips
and cauliflower also contain prominent sulphur compounds.
They are described as strong flavoured vegetables.
Vegetables of the onion family are usually strong
flavoured in the raw state and tend to lose some of the strong
flavours when cooked in water.
Onions contain sulphur compounds that are acted upon
by enzymes in the tissues when the vegetable is peeled or cut to
eventually produce the volatile sulphur compounds that irritate
the eyes and give biting and burning sensations on the tongue.
Vegetables of the cabbage family (cauliflower, cabbage,
knolkhol) are relatively mild when raw but develop strong
flavours when overcooked or improperly cooked.
An amino acid s-methyl l-cysteine sulphoxide is also
present in raw cabbage and appears to be a precursor of cooked
cabbage flavour.
5.4 PECTIN – ROLE IN GEL FORMATION
Pectin is the term designated to those water-soluble
pectinic acids of varying methyl ester content. They are in
between cell walls in soft tissues of most plants acting as
cementing substances.
In general it is the pulp and not the juice of fruits that
contain pectin. Apples contain abundant pectin in their cores and
47
skin. In the preparation of jams, the cores and skins are cooked
with the pulp for pectin extraction. In citrus fruits, the pectin is
chiefly in the white part of the rind. Other sources are sunflower
seeds, guava and peels of mango and orange.
Heat is essential to extract the pectin. The usual way to
extract the pectin from fruit is to heat the fruit in a small amount
of water. Apples are cut into small pieces or ground with skin or
core left intact and cooked to extract the maximum amount of
pectin. Guavas are sliced thin and cooked with water to extract
pectin.

Some points to be remembered for extracting pectin

• The maximum quantity of pectin is extracted in an acid
solute. If fruits are rich in pectin but low in acidity,
acidifying the solution before cooking increases the
viscosity of the extraction.
• Cooked extractions contain more pectin than raw juices.
• Short periods of cooking (usually 10-20 minutes) yield
extractions of better jellying power than does long
boiling.
The role of pectin in gel formation
The formation of a firm jelly takes place only when
pectin, acid and sugar and water are in definite proportions.
When sugar is added to the pectin solution, it acts as a
dehydrating agent and destabilizes the pectin-water equilibrium
and the pectin conglomerates forming a network of insoluble
fibres. Large amounts of sugar solution can be held in this mesh-
like structure.
The strength of the jelly depends on the structure of
fibres, their continuity and rigidity. The continuity of the
network depends upon the amount of pectin present in the
system and the firmness depends on sugar concentration and
acidity.
A soft jelly can be obtained by decreasing the amount of
sugar. However, the rate of setting is modified by acidity. The
48
fibrils of pectin become tough in the presence of an acid and
thus able to hold the sugar solution in the interfibrillar spaces. If
the amount of acid is large, the fibrils lose their elasticity and as
a result jelly becomes syrupy.

5.5 NEED FOR INCLUSION OF FRUITS AND

VEGETABLES IN THE DAYS MENU
1. Fruits and vegetables provide vitamins and minerals
required for growth and maintenance of health and are
thus termed as protective foods.
2. Roots and tubers provide energy.
3. Vegetables are low in fat and can be used liberally in low
calorie diets for weight reduction.
4. Besides providing nutrients they add variety to the diet.
They make the diet attractive by their texture, flavour
and colour.
5. Fruits and vegetables contain phytochemicals. The term
phytochemicals refers to the wide variety of plant
compounds naturally produced by plants. They include
plant pigments and flavouring substances. Fruits and
vegetables with bright colours viz., yellow, orange, red,
green, blue, purple contain phytochemicals.
Beta-Carotene, Vitamin C and Vitamin E are nutrients
that function as antioxidants. An antioxidant is a substance that
significantly reduces or prevents oxidation of fatty acids and
protein thus preventing cell and tissue damage caused by free
radicals in the body.
Free radicals are unstable molecules resulting from
normal metabolic processes. During these processes oxygen
molecules lose an electron, which creates an unstable (molecule)
thereby causing oxidative stress.
These free radicals attack healthy cells in the body in the
hope of finding another electron to stabilize themselves. This
process can cause damage to healthy cells.
49
 6. Intake of fibrous fruits and vegetables are important as
they :
• give satiety and thereby decrease food intake.
• help in regulating bowel movement.
• low in calorie content.
• reduce blood cholesterol levels.
• promote chewing and decrease rate of ingestion.
7. Vegetable and fruits are used in the preparation of
salads.
All vegetable have their own characteristic texture and
colour. Vegetables can be grated, diced, cubed, or cut into a
variety of shapes to prepare salads. The orange colour of carrots,
the pale green of cabbage, the red colour of tomatoes, the green
peppers and the white colour of cucumber, all indicate the
variety of colours available to make attractive salads.
Vegetable used to prepare salads contribute vitamins and
minerals to the diet. Vegetable salads are an important means of
providing part of the need for fibre.
Fruit salad is a colourful, refreshing and light dessert.
Pineapple, orange segments, apple cubes, papaya cubes, grapes,
bananas, sapotas, mangoes, pomegranates are some of the fruits
which can be used in a salad.

5.6 CONSERVATION OF NUTRIENTS IN

PREPARATION AND COOKING OF VEGETABLES
Loss of nutrients in vegetables begin from preparation
onward and is greater during the cooking process.
1. When fruits and vegetables are peeled the vitamins
present under the skin may be lost.
2. Nutrients are also lost when the edible leaves of carrot
beetroot and outer layer of cabbage are discarded.
3. Vitamin B complex and Vitamin C are water soluble and
are lost when the water in which vegetables are cooked
is discarded. Sodium, potassium and chlorine are also
lost when cooking water is discarded.
50
 4. Vitamin C is lost by oxidation due to exposure of air.
5. During dehydration ascorbic acid and carotene are lost.
6. Addition of soda results in heavy loss of B – Vitamins
during cooking.
Guidelines to minimize nutrient losses during preparation:
1. Wash vegetables before cutting. Soaking or washing
time should be reduced to minimize nutrient loss.
2. Cut vegetables into big pieces so that exposure of
vitamins to water is less while cooking and washing.
3. Use a vegetable peeler to remove skin as it helps remove
only a very thin layer of skin.
4. Use minimum water for cooking. Bring the water to boil
and add the vegetables to cook.
5. Cook vegetables by steaming and pressure-cooking to
conserve nutrients.
6. Cover the vessel with a lid while cooking as it hastens
cooking.
7. Do not use soda while cooking vegetables as it destroys
valuable vitamins.
8. Vegetables salads should be prepared just before serving
to conserve nutrients.
9. Use acids such as lime juice or vinegar to salads as it
prevents loss of Vitamin C since Vitamin C is stable in
acid.

5.7 BROWNING
When fruits and vegetables such as apple, banana, potato
and brinjal are cut, there is a development of brown colour on
the surface due to action of enzymes.
This is known as enzymatic browning. When the tissue
is injured or cut and the cut surface is exposed to air, phenol
oxidise enzymes are released at the surface.
These act with the polyphenols present in the fruits and
oxidises them to orthoquinones, which gives the brown colour to
cut tissues.
51
Browning can be prevented by the following methods :
1. Inactivation of polyphenol oxidise by applying heat.
2. Elimination of oxygen by vacuum packing.
3. Change of pH to prevent enzyme action.
4. Dipping of fruits and vegetables in brine and sugar
solutions.
5. Use of antioxidants such as ascorbic acid to retard
oxidation.

QUESTIONS

Part-A

Fill in the blanks:

1). Roots and tubers are rich in _______

2). __________ is the green pigment of leafy vegetables.
3). _________ is the pigment present in carrot.
4).The pigment present in tomato is _________.
5)._________ are a group of reddish water soluble pigments
occurring in many fruits and vegetables.
6). Anthoxanthins are present in ___________ vegetables.
7).The pigment present in beetroot _________.
Part-B
Write short answers

1). How are vegetables and fruits classified?

2). List the major nutrients present in fruits
52
3). Highlight the importance of fibre rich fruits and vegetables in
the diet.
4). Why does an apple become brown when it is cut?

5). What is browning? How can it be prevented?

6) Write a note on the flavour compound present in vegetable.
Part- C
Write detailed answers
1. Compare the nutrient content of green leafy vegetables
roots and tubers.
2. Justify the need for inclusion of fruits and vegetables on
the day’ s menu.
3. How does cooking affect the nutrient content of
vegetables? How can it be prevented?
4. Discuss the pigments and flavour compounds present in
fruits and vegetables.

53
 6. MILK AND MILK PRODUCTS
The story of milk goes back to the beginning of
civilization itself. Cattle were domesticated even in prehistoric
times and milk was one of the most essential of all foods. Milk
is one of the most complete single foods available in nature for
health and promotion of growth .
Milk is the normal secretion of mammary gland of
mammals. Its purpose in nature is to provide good nourishment
for the young of the particular species producing it. Man has
learnt the art of using milk and milk products as a food for his
well being and has increased the milk producing function of the
animals best adapted as a source of milk for him.
The cow is the principle source of milk for human
consumption in many part of the world; Other animals as
source of milk for human beings are the buffalo, goat, sheep,
camel and mare. In India, more milk is obtained from the
buffalo than the cow. Some amount of goat milk is also
consumed.
6.1 NUTRITIVE VALUE OF MILK
Milk is a complex fluid containing protein, fat,
carbohydrate, vitamins and minrals. The main protein in milk is
casein and it constitutes 3.0 - 3.5 percent of milk.
The fat content of milk varies from 3.5 percent in cow’ s
milk to about 8.0 percent in buffalo’ s milk. Fat is present in the
form of fine globules varying in diameter from 1 to 10 µm
(micrometers).Milk also contains phospholipids and cholesterol.
Lactose is the sugar present in milk. The important
minerals in milk are calcium, phosphorus, sodium and
potassium. Milk is an excellent source of riboflavin and a good
source of Vitamin A. However, milk is a poor source of iron
and ascorbic acid. The small amount of iron present is bio
available.
54
6.2 TYPES OF PROCESSED MILK
Raw milk is processed into the following types of milk.
1. Skim Milk:
Skim milk is whole milk from which fat has been
removed by a cream separator. The quantity of fat is usually
0.05 to 0.1 percent. It contains all other milk nutrients, except
Vitamin A and D, but can be fortified by the addition of these
vitamins.
2. Toned Milk :
Toned milk is prepared by using milk reconstituted from
skim milk powder. Skimmed milk is prepared by removing fat
from milk in a cream separator.
The skimmed milk is then mechanically dried to give
skim milk powder. It is mixed with buffalo milk containing 7
percent fat. The fat content of toned milk should be less than 3
percent.
3. Standardised Milk:
In standardised milk the fat content is maintained at 4.5
percent and soluble non-fat is 8.5 percent. It is prepared from a
mixture of buffalo milk and skim milk.
4. Homogenised Milk:
Homogenisation is a mechanical process that reduces the
size of fat globules by forcing milk through small apertures
under pressure and velocity.
When milk is homogenised, the average size of the
globule will be 2 micrometers. The decrease in the size of fat
globules increases their number and surface area.
The newly formed fat droplets brings about stabilisation
of the milk emulsion and thus prevents rising of the cream.
Homogenised milk has a creamier texture, bland flavour and
whiter appearance.
5. Evaporated Milk:
It is made by evaporating more than half the water from
milk under vacuum, at a temperature of 74° C - 77° C. It is
then fortified with vitamin D, homogenised and filled into cans
55
and sterilized at a temperature of 118° C for 15 minutes and
cooled. The treatment employed lends a brown colour and
characteristic flavour owing to the reaction between sugar and
protein.
6. Condensed Milk:
It is obtained when whole milk is concentrated to about
one-third of its original volume and has about 15 percent sugar
added to it. The preparation of condensed milk involves (i)
filtration and pasteurization of milk, (ii) preheating and
evaporation, (iii) addition of sterilised sugar syrup, (iv)
homogenisation.
7. Flavoured Milk :
It is the milk prepared by the addition of flavour such as
rose, pista, badam, cardamom etc. to pasteurised whole milk.
8. Milk Powder:
Milk powder is prepared by dehydrating whole milk in
drum driers or spray driers. In the case of drum or roller drying,
the milk is filtered, pasteurized, homogenised and then fed into
roller driers which are internally heated with steam.
The dried milk is obtained as a thin sheet and is
powdered. In spray drying, the homogenised milk is blown as a
fine spray into a pre-heated vacuum chamber resulting in fine
dry powder. The milk powder is collected, cooled and packed.

6.3 PHYSICAL PROPERTIES OF MILK

Acidity :
Milk has a pH of about 6.5 to 6.7. The salts of the
minerals – calcium, phosphorus, sodium and potassium help to
maintain this pH level.
Viscosity :
The viscosity of milk is affected by temperature, amount
and nature of dispersion of protein and fat, acidity and the
effects of various enzymes and bacteria. Homogenization
increases the viscosity of milk.
56
Freezing Point :
The freezing point of milk is –0.55° C.
Boiling Point :
Milk boils at 100.2° C.

6.4 PASTEURISATION OF MILK

Milk is a favourable medium for bacterial growth.
Pasteurisation destroys all pathogenic bacteria, including those
causing typhoid, tuberculosis, diphtheria as well as yeasts and
moulds.
Pasteurization is a process which consists of heating
milk to a certain temperature for a definite time to ensure
destruction of harmful bacteria. There are three methods of
pasteurisation.
a) Holding method or Batch process :
In this method, milk is held at 62.8° C for 30 minutes
and then rapidly cooled to prevent multiplication of
surviving bacteria.
b) High temperature short time (HTST) method or
continuous process :
Milk is heated to 71.7° C for not less than 15 seconds.
c) Ultra High temperature method :
Milk is heated to a temperature of 93.4° C for 3 seconds.
Advantages of Pasteurization
• During pasteurization the nutritive value of milk is not
altered to a great extent because the temperature employed is
not high and cooking time is short. However, there is a
slight decrease in heat labile vitamin such as thiamine and
ascorbic acid. Proteins are denatured only slightly and
minerals are not appreciably precipitated.
• It does not produce an unpleasant cooked flavour.
• Shelf life of milk is increased due to a marked decrease in
the total bacterial count.
• Harmful pathogens especially TB bacteria are destroyed.
57
• It inactivates enzymes such as phosphatase and lipase in
milk which adversely affect the quality of milk.
6.5 MILK PRODUCTS
Khoa:
Khoa is prepared by evaporating whole milk in an open
cast iron pan with continuous stirring until it is semi-solid. It is
used extensively in the preparation of Indian sweets.
Cream :
Cream is the fat of milk and is used in the preparation of
sweets. It is made by simmering large quantities of milk until a
thick layer of milk fat and coagulated protein form on the
surface. It can be consumed with or without the addition of
sugar.
Butter :
Butter is obtained from cream by churning. When cream
is churned, the fat globules are destabilised and coalesce until
the milk separates into two phases – viz., the butter and the
aqueous phase. Butter is removed and washed.
Butter is used as a cooking medium in many Indian
recipes. It is one of the main ingredients in cakes, biscuits, icing
and bread.
Ghee :
Ghee is butter oil. It is prepared by melting butter and
separating the moisture from butter by heating. It is used in
preparing Indian sweets, savouries, curries and variety rice like
pulav and biriyani.
Paneer :
Paneer is a soft cheese prepared by addition of lemon
juice or citric acid to hot milk and precipitating the casein. The
liquid released in this process is known as whey and the
resultant curd is tied in a muslin cloth and hung for a day to
squeeze any liquid present in it. The soft cheese (paneer) that is
obtained is used in Indian gravies and pulavs. It is a very good
source of protein.
58
Cheese :
It involves the curdling of milk with enzyme rennet
under microbially controlled condition. Milk is held at about
27° C in vats and a lactic acid culture is added. When the milk
gets acidic, rennet is added to it and the milk is allowed to
coagulate.
The curd formed is cut and heated to about 37° C with
constant stirring to remove the whey. The whey is drained. Salt
is mixed with the curd and it is pressed to remove further
amount of whey.
The cheese formed is coated with paraffin to prevent
loss of moisture. The paraffined cheese is allowed to ripen for
three to six months at temperatures between 45° to 60° C.
Cheese is a concentrated source of protein.
Curd :
Curd is prepared by heating milk to about 50° C. A
teaspoon of curd (starter) from an earlier batch of curd is added
and is mixed thoroughly. The lactic acid bacteria present in the
starter curdles the milk. The bacteria breaks down lactose to
lactic acid thereby increasing the acidity of milk. When the pH
reaches 4.6, the milk protein casein coagulates as curd.
The optimum temperature for the formation of curd is
35° - 40° C and the time needed for curd formation is 8 – 12
hours depending on the atmospheric temperature. Curd is used
as a dressing on salads made from fresh vegetables and
combines well with plain cooked rice.
Yoghurt :
This is a coagulated milk product with curd like
consistency. It is made from partially skimmed or whole milk
and it has a slightly acidic flavour.
In the production of yoghurt, a mixed culture of
Lactobacillus bulgaricus, Streptococcus thermophilus and
Lactobacillus acidophilus is added to pasteurised milk and
incubated at 42° C to 46° C.
59
6.6 ROLE OF MILK AND MILK PRODUCTS IN
COOKERY
• It contributes to the nutritive value of the diet, e.g., milk
shakes, plain milk, flavoured milk, cheese toast.
• Milk adds taste and flavour to the product e.g., payasam,
tea, coffee.
• It acts as a thickening agent along with starch e.g., white
sauce or cream soups.
• Milk is also used in desserts, e.g., ice-cream, puddings
• Curd or buttermilk is used as a leavening agent and to
improve the texture, e.g., dhokla.
• Curd is used as a marinating agent, e.g., marinating
chicken and meat.
• Curd is used as a souring agent, e.g., rava dosa, dry curd
chillies.
• Khoa is used as a binding agent, e.g., carrot halwa.
• Cheese is used as garnishing agent.
• Salted butter milk is used for quenching thirst.

QUESTIONS

Part-A
Fill in the blanks:
1). The main protein present in milk is known as
______________
2). ____________ is the sugar present in milk.
3). The fat content of toned milk is _____________ percent.
4). The process of breaking fat globules is known as
__________.
5). The pH of milk is ________________.
6). The boiling point of milk is ___________ and its freezing
point is ________________.
7).When fat is removed from whole milk, the resultant product
60
 is known as ______________.
8) ______________ is prepared by evaporating whole milk in
an open cast iron pan.
9). _____________ is obtained by melting butter.
10) .In the preparation of cheese, milk is curdled using
____________.

Part-B
Write short answers:
1).What are the nutrients present in milk?
2).Differentiate toned milk and homogenised milk.
3).Write a brief note on the physical properties of milk.

4).Write a short note on homogenisation. Highlight its

advantages.

Part- C
Write detailed answers:
1). Why is pasteurization and homogenization carried out in
milk? Highlight its advantages.
2). Give a detailed account on the various milk products
prepared from milk.
3). Discuss the role of milk and milk product in cookery.

61
 7. FLESH FOODS AND EGG

7.1 NUTRITIVE VALUE AND SELECTION CRITERIA

OF MEAT, POULTRY, EGG AND FISH
Meat :
Meat refers to the flesh of warm blooded, four legged
animals chiefly cattle, sheep and pigs. Meat of sheep which is
under 12 months age is sold as lamb. After the age of 12months,
it is called mutton.
Pork is the meat of swine (pig) slaughtered between the
age 5 and 12 months. Veal is the meat of cattle that is
slaughtered 3 to 14 weeks after birth.
If slaughtered between 14 to 52 weeks the meat is called
calf. Meat obtained from cattle slaughtered one year after birth
is called beef.
Meat is a very good source of protein. The average
protein content of meat varies from 16 – 25 percent. The amino
acid pattern of meat protein is of outstanding nutritive value.
The fat content of meat varies from 5 – 40 percent.
Depending on the type, breed and age of the animal, fat
is distributed throughout meat in small particles of large masses.
Fat deposited uniformly in small sheets in the connective tissue
within the muscle is called “marbling”. This contributes
tenderness and flavour to the meat.
Meat fats are rich in saturated fatty acids. The
cholesterol content of meat is 75 mg / 100 gm. Carbohydrate is
found in small quantities and present in the form of glycogen
and glucose.
Meat is a good source of iron, zinc and phosphorpus. It
also contains sodium and potassium. It is an excellent source of
B-complex vitamin particularly B12 which is absent in plant
foods. Liver is an excellent source of iron and vitamin-A.
62
Changes in Meat :
After slaughtering, the lean tissues undergo a series of
complex physical and chemical changes. As a result muscles
loose their soft pliable nature and become rigid, stiff and
inflexible. This is termed as “rigor mortis”.
Stiff muscle starts to soften and becomes tender when it
is held in a cold room temperature between 0°C to 20°C for 1 –
4 weeks. This is known as “ripening” or “ageing”.
During ageing the humidity of the room is to be
controlled. Tenderness of meat can also be obtained by the use
of mechanical methods such as pounding, cutting and grinding
which break muscle fibre. Addition of salt, vinegar, lime juice
and enzymes viz., papain, bromelin and ficin also help in
tenderising meat.

Changes that occur during cooking:

• On heating, the red pigment turns brown due to the
denaturation of protein pigment.
• Heat treatment also brings about inactivation of
enzymes and denaturation of proteins, which makes
meat tougher. Hence, adopting correct cooking
methods, time and temperature will result in a well-
cooked product.
• Heating results in release of volatile compounds from
both fat and lean meat which contributes to the flavour
and taste of cooked meat.
• Heating melts meat fat which increases palatability of
meat when eaten warm.
• There is loss of water on heating which does not
change the nutritive value but may affect juiciness and
bring about shrinkage in volume and weight.
• Minerals like calcium may be lost in meat dripping due
to the dissolution of calcium from bones. There is loss
of B-vitamins also.
63
Poultry:
The term poultry refers to domestic fowls reared for their
flesh and egg. It includes chicken, duck, geese, turkey, pigeon
etc.
Poultry meat has a high protein content varying from 18
to 25 percent. It contains all the essential amino acids required
for body building.
Fat content of poultry is influenced by age and species of
the bird. Young birds have little fat content. Chicken fat is
unsaturated and is therefore better than the fat of red meat.
Poultry flesh is a good source of B-vitamin and minerals.
Egg :
The term egg mainly refers to the egg of hen and duck.
An average egg weighs 50 gms. approximately and is composed
of the shell, egg white and yolk. The weight is distributed in the
different parts as follows.
TABLE – 7 A
Percentage composition of egg
Part Weight%
Shell 8 – 11
White 55 – 61
Yolk 27 - 32
Egg is a rich source of protein and lipids. Egg protein is
of high quality as compared to any dietary protein and therefore
is used as a standard for evaluating the protein quality of other
foods. The nutrient composition of egg white and yolk differ
considerably and is represented in the following table.
TABLE – 7 B
Percentage nutrient composition of egg white and yolk
Nutrient Egg white Egg yolk
Water 88.0 % 48.0 %
Protein 11.0 % 17.5 %
Fat 0.2 % 22.5 %
Mineral 0.8 % 2.0 %

64
Vitamin and minerals in Egg:
Egg yolk is rich in vitamin-A. Thiamin and riboflavin
are present in appreciable amounts. Calcium is present in the
yolk in small amounts.
Phosphorus is abundant in the yolk. Eggs are an
important source of bioavailable iron and a fair source of
sodium, magnesium chlorine, potassium and sulphur.
Fish:
Fishes are classified as shell fish and fin fish. The
nutrient composition of fish of a given species varies depending
on the season of year and maturity. However most fish contain
15 – 24 percent protein, 0.1 – 22 percent fat and 0.8 – 2 percent
minerals.
Fish proteins are easily digestable and are of high
biological value. The fat content is influenced by the species,
feeding habits and maturity of fish. Glycogen is present in fish
but is in lesser quantities than meat.
Fish oils are an excellent source of vitamin A and D.
They are a good source of thiamin, riboflavin and niacin. Small
fishes that can be eaten with bones contribute a significant
amount of calcium.
Ocean fish are a rich source of iodine. Oysters are an
excellent source of copper. Fishes contain omega – 3
polyunsaturated fatty acids. These acids are found to prevent
the degeneration diseases of the heart.
SELECTION CRITERIA:
Meat:
Veal or the meat of calf is pale pink and firm. The meat
is soft and flabby and the cut surface is moist. The bone is
pinkish white with a small quantity of blood. Fat is not seen.
In the case of lamb, flesh of young animals below 12
months of age, the meat is pinkish red, fine grained and velvetty.
The bones are porous and reddish. Good quality mutton is deep
red in colour with a smooth covering of fat. The bone is hard
and white.
65
 Poor quality meats have darker flesh and the grain is
coarse and fibrous. Fat layers are heavy and have a strong
flavour.
Poultry :
Young birds (below 9 months of age) have a tender flesh
and are more suitable for cooking.
Signs of a young bird :
• The feathers especially the quills on the wings should be
easy to pull.
• No long hair on the body.
• Skin should be white or clean and smooth.
• Feet should be supple with smooth even over-lapping
scales.
Signs which show that a bird is fresh :
• Feathers – light and fluffy.
• Eyes – prominent and clean
• No unpleasant smell
• Feet moist and not stiff and dry.
• Skin – clear with no dark or greenish tinge.
Egg:
• The shell should be clean and should not be broken.
• Fresh eggs have a small air cell. This can be detected by
holding the egg against light.
• Yolk should be in the centre without any dark spots when
observed against light.
• Good quality egg will sink when immersed in water. Poor
quality eggs will float due to the enlarged air cell and loss
of moisture.
Fish:
The following points should be borne in mind while
selecting fish :
i) Eyes should be bright and not sunken.
ii) Gills should be red.
66
 iii) The tail should be stiff and scales firmly attached to the
skin.
iv) The flesh should be firm and not flabby.
v) There should be no unpleasant odour.
vi) To test a cut piece, press down with a finger and if an
impression is left then the fish is stale.
vii) Any tendency for the raw flesh to come away from the
bone is a dangerous sign.

7.2 USE OF EGG IN COOKERY

As a Thickening Agent :
Egg proteins coagulate on heating. The coagulation of
protein is accompanied by binding of moisture and increase in
viscosity. Therefore eggs can be used as thickening agents.
As a Binding Agent :
Egg protein coagulate between 65 and 70° C and help to
hold shape of the products such as cutlets in which it is used.
As a Leavening Agent :
Eggs when beaten, form elastic films which can trap air.
This air expands during baking and gives a fluffy spongy
product.
As a Emulsifying Agent :
Besides protein, egg contains phospholids such as
lecithin which are known for their emulsifying quality. Hence
egg can be used an excellent emulsifying agent in products such
as mayonnaise as it is able to stabilise the oil in water
dispersion.

As a Flavouring and Colouring Agent :

Egg is used in food mixtures to contribute flavour and
colour to products such as cakes and pudding
As a Clarifying Agent:
Egg helps in the preparation of clear soups. When a
small amount of egg white is added to the liquid and heated, the
67
egg albumin coagulates and carries along with it suspended
particles. On allowing it to settle, a clear soup is obtained.
As a Garnishing Agent :
Hard boiled eggs are diced and are used to garnish dishes
like biryani.
As an Enriching Agent :
Eggs are used to enhance the nutritive value of various
preparations.
QUESTIONS
Part-A
Fill in the blanks:
1). The protein content of meat varies from __________ to
_______ percent.
2). Liver is a rich source of vitamin ____________.
3). _________________ present in fishes helps to prevent
degenerative diseases of the heart.
4). The colour of good quality mutton is ______________.
5). Fresh eggs should __________ when immersed in water.
6). Yolk should be in the ______________ when observed
against light.
Part-B
Write short answers:
1). What are the changes that occur in meat after slaughtering?
2). Compare the nutritive value of egg white and egg yolk.
Part- C
Write detailed answers:
1). Discuss the nutrient content of meat, poultry, and fish.
2). What are the factors to be considered while selecting:
a) Fish
b) Poultry
c) Meat
d) Egg
3. Highlight the nutrient content of egg. Give a detailed account
on the uses of egg in cookery.
68
 8. NUTS AND OIL SEEDS
8.1 NUTRITIVE VALUE OF NUTS
Nuts are a rich source of protein and fat and a good
source of B – Vitamin and antioxidant vitamin E. They are a
concentrated source of energy.
Groundnut, cashewnut, coconut and almonds are the nuts
commoly used in India and their nutritive value is given in
table 8A
Ø Groundnuts are a very rich source of protein and fat.
They are exceptionally rich in niacin. Groundnuts are
boiled or roasted and consumed.
It is also used in the preparation of groundnut butter.
The chief product is the oil which can be used for
cooking. The cake left after the oil is extracted is
purified and used in supplementary mixes.
Ø Cashewnuts are also a rich source of protein and fat and
contain appreciable amount of iron. It is widely used in
the preparation of sweets and confectionery.
It can be roasted and eaten. Cashewnuts are also
used to garnish dishes such as pulavs and payasam.
Ø The white flesh of coconut is rich in calories though not
a very good source of protein. It is extensively used in
cookery in Tamil Nadu and Kerala in the preparation of
curries, chutneys, sweets and puddings.
The white flesh when dried is called copra and has a
high content of oil.
Ø Almonds are expensive and are used in the preparation
of badam milk and sweets. It is a rich source of protein
that are not of high biological value.
Almonds are an excellent source of vitamin E, an
antioxidant.

69
 TABLE 8 A
Nutritive value of nuts per 100 gm.

70
8.2 OIL SEEDS
Groundnut :
Groundnut oil which is obtained from groundnut is a
clear amber coloured liquid which is extensively used in
cooking. It is used in the preparation of margarine, vanaspathi
and preservation of sardine fish.
The residue left after the oil extraction of groundnut is
the groundnut cake, which is used in the preparation of
groundnut flour and cattle feed for farm animals.
Coconut :
Coconut oil is extracted from dried coconut. It can also be
used in the preparation of vanaspathi and margarine. Coconut
cake obtained after oil extraction is a valuable feed for cattle.
Soyabean :
Soyabean yields substantial amount of oil. The whole
dry beans contain 40 percent protein and 20 percent fat. In
India, soyabean oil finds its application in the manufacture of
vanaspathi.
The meal after oil extraction is being used widely as a
poultry feed. The protein isolated from soyabean meal after oil
extraction is being successfully incorporated in weaning and
supplementary foods.
Sesame seeds :
Gingelly oil is extracted from sesame seed and it is a
traditional oil that has been used in India. The roasted seeds are
mixed with jaggery syrup and made into balls and eaten.
Decorticated seeds contain 25 percent oil and are used
for oil extraction. Sesame seeds are a fair source of protein and
calcium.
8.3 ROLE OF NUTS IN COOKERY
Nuts and oilseeds are used in cookery as whole, halved, ground
or desiccated.

71
 • Nuts are used in fresh, raw, roasted, boiled or salted
forms and also fried forms.
• Nuts are used as thickening agents. Coconut and
cashewnuts are used as thickening agents in the
preparation of gravy.
• Chutneys can be made and used from nuts, e.g.,
groundnut and coconut.
• Sweets can be made from nuts, e.g., cashewnut and
coconut burfi.
• Powders made out of nuts like groundnut and coconut
are used as chutneys and salad dressing.
• Nuts are used to garnish ice-creams and cakes
• Nuts are used in beverages, e.g, badam kheer.
• Peanut butter is used as a topping on bread or as side
dish along with chapathis.

QUESTIONS
Part-A
Fill in the blanks:
1). Nuts are a rich source of ________ and _______
2). Groundnuts contain ________ gram of protein.
3). The iron content of cashewnut is _________ gram
4). The protein content of almond is _________gram.
5). Groundnuts are exceptionally rich in vitamin ________

Part B
Write short answers
1). Compare the nutritive value of groundnut and coconut.
2). Write a brief note on sesame seeds and groundnut.
Part- C
Write detailed answers
1). Give a detailed account on the nutritional; significance and
use of nuts and oil seeds.
72
 9. FATS AND OILS
Fats are an important component of the diet and is
present naturally in many foods. Fats are solid at room
temperature while oils are liquid. Fats in the diet can be of two
kinds viz., the visible and the invisible fat.
Invisible fats are those present inherently in foods.
Example of food containing appreciable quantities of invisible
fat include meat, poultry, fish, dairy products, eggs, nuts and
seeds.
Visible fats are those fats that are made from these
products. They are cooking oils, salad oils, butter, ghee and
margarine.

9.1 NUTRITIONAL SIGNIFICANCE

• They are a concentrated source of energy. One gram of fat
contributes 9 kilocalories as against 4 kilocalories
contributed by carbohydrates and protein.
• They are a good source of vitamin A,D,E and K.
• They provide essential fatty acids which are components of
membranes of living cells.
• They impart special flavour and texture to our foods, thus
increasing palatability.
• They are also used by the body to make prostoglandins
involved in a large variety of vital physiological functions.
9.2 REFINED OILS
Oils and fats do not occur free in nature. They occur in
animal tissues and in seeds and fruits from which they are
isolated.
The extracted oils are crude and contain many
constituents like free fatty acids, unsaponifiable matter, gums,
waxes, mucilaginous matter, variety of colouring matter,
73
metallic contaminants and undesirable odour producing
constituents. In refining these constituents are removed by the
following steps:
• Suspended particles are removed by filtration or
centrifugation.
• Free fatty acids are removed by alkali treatment.
• Any remaining free fatty acids are removed by
neutralisation.
• Pigments are removed by bleaching using adsorbents like
activated earth or carbon and sometimes chemical bleaching
agents.
• The oil is finally deodorized by injecting steam through the
heated fat under reduced pressure to obtain refined oil.

9.3 HYDROGENATION – VANASPATHI AND

MARGARINE
Plant oils contain a large percentage of unsaturated fatty
acids and hence have a tendency to become rancid. These
unsaturated glycerides in oil can be converted to more saturated
glycerides by the addition of hydrogen. This process is known
as hydrogenation.
Hydrogenated fat is manufactured from vegetable oils by
the addition of molecular hydrogen to the double bonds in the
unsaturated fatty acids in the presence of nickel.
The double bonds take up hydrogen and saturated fatty
acids are obtained. By this process, liquid fats can be converted
to semi solid and solid fats for use as shortening in the
preparation of biscuits, cakes and butter substitutes.
Hydrogenation is of great economic importance because
it allows oils to be converted into fats, which have better
keeping quality.
As hydrogenated fats are prepared from refined
deodourised oils, the resulting fats are odourless and colourless
and blend well in several food preparations.
74
Vanaspathi:
Hydrogenated oil in India is known as vanaspathi. It is
manufactured by hydrogenating refined groundnut oil or a
mixture of groundnut oil with other edible vegetable oils.
According to vanaspathi control order, the melting point
of vanaspathi should be between 31°C and 37° C and it should
contain 5 percent sesame oil and should be fortified with
vitamin A.
Margarine :
Margarine is often used as a substitute for butter. It is
made from vegetable oils or a mixture of vegetable and animal
fat by hydrogenation.
It is then blended with cultured skim milk and salt. The
fats most commonly used in the manufacture of margarine are
cotton seed oil, soyabean oil, corn oil, groundnut oil, coconut oil
and meat fat.
Additional additives may include diacetyl for butter
flavour, sodium benzoate for preservation, mono and
diglycerides or lecithin for emulsification, yellow colouring
matter and vitamin A and D.

9.4 RANCIDITY
Fats and oils undergo certain undesirable changes during
storage which result in spoilage. The major spoilage of fats and
oils is rancidity.
Rancidity refers to the development of disagreeable
odour and flavour in fats and oils owing to specified chemical
reaction such as oxidation and hydrolysis.
Hydrolysis is the decomposition of fats into free fatty
acid and glycerol by enzymes in the presence of moisture.
These free fatty acids released are responsible for the unpleasant
flavour and odour.
During oxidation, oxygen is added to the unsaturated
linkage and this results in the formation of peroxides. These
peroxides decompose to yield aldehyde and ketones which are
75
responsible for the pronounced off flavour. Rancidity may also
be caused by the absorption of odour and action of micro
organism and enzymes.

Prevention of rancidity :
• Storage in coloured glass containers prevent oxidation of
fats by rays of light.
• Vacuum packaging retards rancidity by excluding
oxygen.
• Naturally occurring antioxidants like vitamin C, β
carotene and vitamin E protect against rancidity.
• Synthetic antioxidants like butylated hydroxy anisole
(BHA), butylated hydroxy toluene (BHT) and
propylgallate can also be added to prevent rancidity.

9.5 SMOKING POINT

When fats and oils are heated to a high temperature,
decomposition of fat occur and finally a point is reached at
which visible fumes are given off. This is called smoking
point and the temperature is called smoking temperatures of
fat.
Smoking temperature is defined as the lowest
temperature at which visible fumes consisting of volatile
gaseous products of decomposition are evolved.
Factors affecting smoking temperature of fats and oils :
1. The amount of free fatty acids present.
2. The surface of oil exposed while heating.
3. The presence of mono and diglycerides and foreign
particles such as flour particles.
Smoking temperature is important for fats used for
frying. Fats with low smoke point are not suitable for frying
because of the odour and irritating effect of the fumes.
The decomposition products may also give an unpleasant
flavour to the food. Hence it is preferable to use fats with
relatively high smoking temperatures for frying.
76
 The smoking points of some fats and oils are given
below :
TABLE – 9 A
Smoking points of some fats and oils

Oil or fat Smoking temperature (°° C)

Soyabean oil 230
Hydrogenated fat 221
Butter fat 208
Groundnut oil 162
Coconut oil 138

9.6 ROLE OF FAT / OIL IN COOKERY

• Fat is used as a medium of cooking in shallow and deep
fat frying.
• Fat improves the texture of food. e.g., cake, biscuit,
cookies.
• Fats help in leavening – in making cake, leavening
occurs by incorporating air into the fat during the
leavening process.
• Fat increases smoothness of the product e.g., Halwas,
crystalline candies.
• Fats are shortening agents- one of the most important
function of fat is to shorten baked products which
otherwise are solid masses firmly held together by
strands of gluten.
• Fat improves palatability – fat gives taste and flavour to
the food.

77
 QUESTIONS
Part-A
Fill in the blanks
1). Fats contribute _____________ kilocalories per 100 gm.
2). In hydrogenation ___________ fatty acids are converted to
____________ fatty acids by the addition of hydrogen.
3) Hydrogenated oil in India is known as ______________.
4). _____________ is a good substitute for butter.
5). The development of disagreeable flavours and odours in fat
is known as __________.
6). Fats with ____________ smoking point are suitable for
frying.

Part-B
Write short answers
1).What are visible and invisible fats? Discuss the role of fats in
cookery
2). Explain the following terms
(a) hydrogenation, (b) randicity
3). How do fats and oils become rancid? How can it be
prevented?
4) Discuss the role of fats in cooking

Part- C
Write detailed answers
1). Discuss the nutritional significance of fats and oils. Explain
the role in cookery
2). Explain the following
(a) refined oils (b) Hydrogenation (c) Rancidity.
3). Define smoking point. Give the smoking points of some fats
and oils and justify your choice of oil for deep-frying food.

78
 10. SPICES AND CONDIMENTS

Spices and condiments are indispensable components of

Indian cuisines. These are accessory foods mainly used for
flavouring food preparation to improve their palatability.
These are used in small amounts and their contribution to
nutrient intake is very limited. Some of the spices are rich in
iron, trace metals and potassium.
Some condiments like chillies and coriander are a fair
source of β - carotene. Green chillies provide β - carotene and
vitamin – C.
Spices are aromatic plant products used to season food.
Condiments are seasonings added to the food at the table, eg.
salt. However, there is no clear cut division between the two.

10.1 SPICES AND CONDIMENTS IN INDIAN

COOKERY
The common spices and condiments used in Indian cookery is
discussed in the following table.
TABLE –10.A
Spices and Condiments in Indian Cookery

Sl Spices Usage
No
1. Ajwain Used in curries, pickles, biscuits,
(omum) beverages, confectionery due to its
characteristic aroma and pungent taste.
Medicinal use :
Antispasmodic, stimulant used for
treating digestive disorders.

79
2. Aniseed Used for flavouring curries, cakes,
(somfu) cookies, biscuits. It is served fresh and
coated with sugar syrup as a mouth
freshner at the end of an Indian meal.
Medicinal use :
Counteracts flatulence, used in treating
colic pain.
3. Asafoetida Used in seasoning rasam, sambhar,
lemon rice and for flavouring curries,
sauces and pickles.
Medicinal use : Used in treating
bronchitis and whooping cough. It
counteracts intestinal flatulence.
4. Bay leaves Used for flavouring vinegar and
(Birinji seasoning biriyani, pulav, soup, meat
leaves) preparation.
5. Cardamom Used for flavouring curries, cakes,
(Elaichi) sweets, beverages and bread.
Medicinal use : Appetiser and effective
remedy for indigestion and urinary
disorders.
6. Coriander Flavouring in pickles, sausges, seasoning
seeds and confectionery.
(dhania) Medicinal use : Used in flatulence,
vomiting and intestinal disorders,
recommended in hypertension.
7. Chillies Essential ingredient in Indian curries.
Used in making chutneys, pickles and all
vegetable dishes.
8. Fenugreek Important component of masala powder
seeds and idli batter. Although bitter it
(Methi) enhances the flavour of pickles and
gravies.
Medicinal use : Reduces blood sugar
levels, used to treat dysentery.
80
9. Garlic Used in chutneys, pickles, meat
preparations, tomato ketchup, pulav,
rasam etc.
Medicinal use : Lowers blood
cholesterol levels, used to treat digestive
disorders, lowers blood pressure, inhibits
the growth of fungi belonging to
Aspergillus and Candida, has
antibacterial properties and is a good
antibiotic. Used to treat skin disease and
ear ache. It is also anticarcinogenic.
10. Ginger Used as a component of pickles, curry
paste/powder, chopped ingredient in
spicing dishes like uppuma and in
making ginger bread, ginger biscuit,
ginger candy, ginger squash.
Medicinal use : Reduces inflammation
and pain in joints, used to treat migraine
headaches and helps to relieve nausea.
11. Nutmeg Used for flavouring puddings and fruit
pie.
Medicinal Use : Used for its anti-
microbial properties.
12. Mace Flavouring puddings and fruit pie, fish
sauces, meat preparation, pickles. It is
ground and used in cakes, cookies,
chocolate dishes.
Medicinal use : Used for its anti-
microbial property.
13. Onion Raw rings used in salads and raita.
Essential ingredient in curries and fried
foods. Used in preparation of chutneys
and pickles. Onion powder is a
flavouring agent.

81
 Medical use : Used to lower blood
glucose levels. It has antibacterial
properties. Onion can lower blood
cholesterol levels.
14. Poppy seeds Used for topping bread, cake, rolls or
buns. Oil extract is used for salads. Used
for thickening gravies and non-
vegetarian dishes.
15. Pepper Seasoning foods like vadai, bonda, and
pongal, used as a component of masala
powder, used in pickling meat and in
preparation of stews. Enhances flavour
of salads, sandwich, papads, soups and
omlettes.
Medicinal use : Used in hot-milk for
throat infection. Used in relieving body
ailments.
16. Saffron Essential garnish of mughalai dishes,
(kesar) mainly pulav and biriyani. Used as
colouring agent (yellow), used in soups,
sauces and sweets like rasamalai and
srikand to give yellow colour.
Medicinal Use : Used as a sedative and
in eye infections.
17. Tamarind Used for flavouring sambhar and rasam.
Used as souring agent in chutney pickle
and tamarind rice and as a thickening
agent in gravies.
18. Turmeric Colouring agent (yellow) and flavouring
agent in all gravies, essential component
of curry powders. Fresh foods are
applied with turmeric paste after
cleaning as it is a natural disinfectant.
Medicinal use : It is a natural
disinfectant. It is an antioxidant and
reduces the
82 progress of cancer. It is used
for relieving stomach irritation, sore
throat, cough, cold and flatulence, helps
 reduces the progress of cancer. It is used
for relieving stomach irritation, sore
throat, cough, cold and flatulence, helps
to reduce blood cholesterol levels.
19. Mustard Seasoning curries and pickles, chutneys.
seeds Medicinal use : Used as body massage
oil.
20. Cumin Flavouring curries, rasam, pickles,
(Jeera) bread, sauces and cakes.
Medicinal use : Stimulant and diuretic.
Increases the levels of anticancer
enzymes in the body.
21. Cinnamon Used in curry powders, seasoning meat
preparations and is an ingredient in
confectionery.
22. Clove Used as a table spice, used in preparation
of curry powder, flavouring agent in
pulav and biriyani.
Medicinal use: Used in tooth pain. aids
digestion, relieves muscular cramps and
headache.
23. Curry leaf Seasoning in almost all south Indian
dishes like rasam, sambhar, gravies and
snacks like boondhi mixture, used in
preparation of chutneys and powders.
24. Coriander Used raw in salads and chutneys. Used
leaf as a flavouring agent and garnishing
agent.

10.2 USES IN COOKERY

The role of spices and condiments in cooking are as
follows.

83
 Table 10 B
The role of spices and condiments in cooking
1. Flavouring Eg. Cardamom adds flavour to
Agent payasam.
2. Colouring Eg. Turmeric gives colour to lime rice.
Agent
3. Preservative Eg. Garlic, turmeric, fenugreek
powder retard the growth and
multiplication of bacteria in pickles.
4. Souring Agent Eg. Tamarind lends a sour taste to
gravies, chutneys and pickles
5. Thickening Eg. Poppy seeds, ginger garlic paste
Agent are used to thicken gravies.
6. Medicinal Eg. Coriander seed, cumin seeds aid
Agents digestion while garlic, onion are used
to reduce blood sugar and cholesterol
leaves.
7. Stimulants Spices tend to increase appetite and
therefore food intake.
8. Imparts variety Different spices lend different
in foods flavours, colours and taste to food.
9. Gives Chillies added to chutneys and ginger
pungency added to lime juice and tea give a
characteristic burning sensory
stimulation.

84
 QUESTIONS

Part-B
Write short answers

1). What are the different spices used in our food?

2). What is the role of spices in food preparation?

Part- C

Write detailed answers

1). Discuss the medicinal value of spices and condiments in

detail.

2). Explain the role of spices and condiments in food

preparation.

85
 11. SUGAR, JAGGERY AND HONEY
11.1 NUTRITIVE VALUE

Sugar, honey and jaggery are sweetening agents. They

are added to beverages and foods to increase palatability. Sugar
is made up of glucose and fructose.
It is a source of energy providing 4 kilocalories per
gram. Jaggery is made from sugar cane juice after processing it.
Jaggery is a fair source of iron. Palmyra palm, date palm or
coconut palm is used for it.
Honey is the golden coloured syrup made by bees from
the nectar of flowers. It is a mixture of glucose and fructose.
The nutritive value of sugar, honey and jaggery are
given below:

TABLE – 11.A
Nutritive value per 100 g
Item Energy Carbohydrate Calcium Iron
(k cal) (g) (mg) (mg)
Sugar 398 99.4 12 0.15
Jaggery 383 95.0 80 2.65
Honey 313 79.5 5 0.69

Source : Gopalan. C.,Rama Sastri B.V., Balasubramanian S.C.,

(1991) - Nutritive value of Indian Foods, National
Institute of Nutrition, ICMR, Hyderabad.

11.2 STAGES OF SUGAR COOKERY

When sugar is boiled at different temperatures various
products can be obtained.

86
 TABLE – 11.B
STAGES OF SUGAR COOKERY

Product Temperature Test Description of

Test
Syrup 110 – 112°C Thread When syrup is
dropped from a
spoon, syrup spins
a 5 cm thread.
Barfi, 112 – 115°C Soft ball Forms a soft ball
fondant, when syrup is
fudge dropped in cold
water.
Caramels 118 – 120°C Firm ball Forms a firm ball
when syrup is
dropped into cold
water, does not
flatten on removal
from water.
Divinity, 120 – 130°C Hard ball Forms a ball hard
laddu, enough to hold its
marsh- shape when syrup
mellow is dropped in cold
water.
Butter- 132 – 143°C Soft Forms threads
scotch crack which are hard but
toffees not brittle when
syrup is dropped in
cold water.
Brittle 150 – 154°C Hard Forms threads
crack which are brittle
when syrup is
dropped.

87
 Barley 160°C clear Sugar melts
sugar liquid
Caramel 170°C brown Sugar melts and
liquid browns.

Source : Shakuntala Manay N, Shadaksharaswamy. M (1987) -

Foods, Facts and Principle, New Age International (P) Limited,
New Delhi.
Two types of confectionery can be prepared when sugar
is boiled viz., crystalline candies and amorphous candies.
Crystalline candies can be made by boiling sugar and
water sufficiently to concentrate sugar to the point where a firm
crystalline structure will form on cooling.
During preparation, butter and flavouring agents are
added to enhance the quality of the product. They can be
chewed and may easily be cut with a knife. They have a smooth
velvetty texture. Eg. fondants and fudges.
Amorphous candies are boiled until they reach a high
temperature. Concentration of sugar is far greater in amorphous
candies (>90%) than in crystalline candies.
This high concentration of sugar prevents organisation of
sugar crystals, hence the term amorphous candies. These
candies easily crack.
Preparation of crystalline confectionary
Fondant:
Two hundred grams of sugar is dissolved in 120 ml of
water and boiled till 113° C – 114° C. Cream of tartar and
glucose are added and the mixture is concentrated by boiling
until it reaches appropriate doneness.
The doneness of the candy is determined by measuring the
temperature (113° C – 114° C) of the boiling solution. Another
method of measuring doneness in the making of candies is by
dropping a small portion of boiling syrup into very cold water
allowing the syrup to cool and evaluating its consistency. In the
case of fondant it is a soft ball shape.
88
 The boiling solution is then poured on a smooth flat
surface and allowed to cool at 40° C. Then it is beaten until it
becomes a creamy mixture and ripened for 24 hours to promote
smoothness.
Fudge:
In the preparation of fudge, butter and milk are added to
prevent crystallisation. Fat and sugar are added to the solution
after it reaches the boiling point (117° C). Apart from these
changes, the principles of making fudge do not differ from those
of making fondant.

Preparation of Non-Crystalline Confectionary

Crystallisation of sugar is prevented by cooking the
solution to a high temperature so that the product hardens
before crystals are formed. During cooking a brown colour
develops due to the caramelisation of sugar.
Caramels are made by adding corn syrup, fats and
concentrated milk products to the sugar syrup.
Brittles are made by melting and caramelisation of
sugar. Toffee is made from sugar syrup with the addition of
cream of tartar, vinegar or lemon juice. Gelatin is used in the
preparation of spongy candies like marsh mellows.

11.3 ARTIFICIAL SWEETENERS

Artificial sweeteners are those substances used as
substitutes for sugar.
Characteristics of an ideal sweetener :
• sweet or sweeter than sucrose.
• pleasant taste with no after taste.
• colourless
• odourless
• readily soluble
• stable
• economical
89
 • non toxic
• does not promote dental caries.
Low calorie sweeteners :
Polyols :
Polyols occur in nature and are also synthesised from
easily accessible carbohydrates such as starch, sucrose, glucose,
invert sugar, xylose and lactose.
Sugar alcohols (xylitol, sorbitol, mannitol, lactitol and
isomalt) are polyols. They are white crystalline, water soluble
powders. Sorbitol is used in chocolates and diabetic foods.
Mannitol is used in sugar free chewing gum and xylitol is used
in pastries, jam, ice cream. The average calorific value is
2.4 k cal / g.

Non-caloric Sweeteners :
Cyclamate :
It is 30 times sweeter than sucrose. It was banned as
studies revealed its role in the development of tumors.
Acelsulfame – K :
It can be used in cooked or baked products. It is a
synthetic derivative of acetoacetic acid.
Alitame:
It is a dipeptide based amide. It is 2000 times sweeter
than sucrose.
Aspartame :
It is made by combining two amino acids – aspartic acid
and phenyl alanine. Methyl alcohol is then added to form a
methyl ester. It is a white, crystalline powder that has a sugar
like taste. It is used in soft drinks, instant tea and coffee.

Saccharin:
It is sodium orthobenzene sulphonamide or its calcium
salt. It is 300 times sweeter than sucrose. According to the
WHO recommendation only 0 – 2.5 mg / kg body weight is
permitted daily.
90
Natural Non-Caloric Sweeteners :
Neohespiridine dihydrochalcone – It is isolated from
citrus peel.
Glycyrrhizin – It is obtained from roots of leguminous
plant Glycyrrhiza glabra.
Thaumatin – It is obtained from a West African fruit
plant.

11.4 ROLE OF SUGAR IN COOKERY

1. It is used as a sweetening agent.
2. Used in the preparation of sugar syrup for sweets like
gulab jamuns, fruit squashes.
3. It is a preservative in jams and jellies. High
concentration of sugar prevents the growth of micro-
organisms.
4. Sugar contributes to the flavour and colour of the
product when it is caramelised.
5. It helps to improve texture of cake and confectionary.
6. It can be used to prepare sweets and candies.

QUESTIONS

Part-A
Fill in the blanks:
1). Sugar provides ________________ k cal / g.
2). The iron content of jaggery is __________________.
3). ________________ confectionary cracks into pieces.

4). Fudge is a ______________________ confectionary.

5). Caramel is obtained when sugar is boiled to ________° C.

6). A hard ball is obtained at ______________ temperature.

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7). The calorific value of polyol is ________________.
8). ____________ is a synthetic derivative of acetoacetic acid
and is used as a sweetener.

Part-B
Write short answers
1). Compare the nutritive value of sugar, honey and jaggery.

2). What is the difference between crystalline and non-

crystalline confectionary?
3). How are fondants prepared?
4) Give the characteristics of artificial sweeteners
Part- C

Write detailed answers

1). Explain the various stages of sugar cookery. Highlight the
importance of sugar in cookery.
2). Write a brief note on the different types of artificial
sweeteners. What are the characteristics of an ideal
sweetener?

92
 12. BEVERAGES AND APPETIZERS

Beverages are drinks used for the purpose of relieving

thirst and including fluid in the days diet. They contain
nutrients and are also stimulants.
Appetizers are those liquids that improve the appetite of
an individual. Eg. Soups.
Intake of appetizers before a meal tends to increase the
quantity of food consumed and also stimulates a desire for food
consumption.

12.1 USE IN A DAYS MENU

1. Refreshment :
Beverages such as plain or carbonated water, lime juice,
ginger ale and other bottled beverages, fruit juices and iced tea
or coffee are refreshing drinks and are used to relieve thirst.
2. Nourishment :
Pasteurized milk, butter milk, chocolate and cocoa
drinks, eggnog made with rum, fruit juices, glucose water,
lemonade provide nutrients and help in nourishing the body.
3. Stimulant :
Tea, coffee, cocoa and chocolate beverages help in
stimulating the system.
4. Soothing Agent:
Warm milk and hot tea have a soothing effect and are
used for this purpose.
5. Appetizers :
Soups, fruit juice and alcoholic drinks in limited
quantities increase an individuals appetite and thereby food
consumption.

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12.2 NON ALCOHOLIC BEVERAGES :
Tea :
Tea is obtained from the leaves and flowers of tea bush.
The kind of tea obtained is determined by the manufacturing
process and treatment. Tea is a stimulating and refreshing drink.
The principle flavour components of tea are caffeine,
tannin yielding compounds and small amounts of essential oils.
Caffeine provides the stimulating effect, tannin the colour, body
and taste to the extract and the essential oils contribute the
characteristic aroma.
Tea can be prepared by introducing tea leaves into
boiling water in a kettle or by pouring boiling water over tea
leaves in a preheated tea pot and letting it steep.
The time of steeping depends on the strength of the
beverage desired and quantity of tea leaves used. To prepare
good tea one teaspoonful of tea leaves for 1 cup is ideal.
The tea should be brewed only for five minutes and
strained. If it is kept for more than five minutes it will give a
bitter taste. Milk and sugar should be added to individual taste.
Coffee:
Coffee is prepared from the beans of the coffee plant.
Caffeine and flavouring substances such as tannins determine
the quality of the end products.
Coffee can be prepared by filtration and percolation.
Filtration:
This is a very common method used in the preparation of
coffee. A coffee filter is used for this purpose. Coffee powder
is placed in the top part of the filter and covered with a disc,
which is perforated.
Boiling water is poured over the coffee powder and it
drift through it and extracts the flavour and aroma.
Percolation :
In this method the heated water is forced upward through
a tube into the coffee compartment. When it filters through, it
extracts the coffee flavour.
94
 The water filters through the coffee several times before
the desirable strength is obtained.

Fig12.1 Coffee filter

Source: Sumati Mudambi, R Shalini Rao M.,1989,Food
Science, New Age International (p) Publishers Limited

Fig 12.2 Percolation

Source: Sumati Mudambi, R Shalini Rao M.,1989,Food
Science, New Age International (p) Publishers Limited
95
Instant coffee :
Instant coffee is prepared by pouring boiling water over
instant coffee powder in a dry cup.
Cocoa And Chocolate:
Cocoa and chocolate are made from grinding the seeds
of cocoa bean pods. Chocolate and cocoa unlike coffee and tea
have a higher nutritive value.
The percentage nutritive value of chocolate and cocoa
are given in the following table.

TABLE – 12.A
Percentage nutrient composition of cocoa and chocolate

Constituents Cocoa Chocolate

Water 4.6 5.9
Protein 21.9 12.9
Fat 28.6 48.7
Carbohydrates 32.7 30.0

Cocoa should always be mixed with a small amount of

cold liquid before being combined with other ingredients. This
prevents the formation of lumps.
It is then heated to boiling and held at that temperature to
extract the flavour and reduce the amount of sediment that
settles at the bottom.
Fruit Beverages :
Fruit beverages are obtained by extracting the juice from
fruits such as orange, grape, pineapple, lemon, tomato etc.
These juices are an excellent source of vitamins,
minerals and energy depending on the fruit used. Fruit juices
are not only refreshing, they are nutritious, and increase fluid
intake.
Fruit squashes are prepared by combining sugar syrup
and fruit juice. They have a long shelf life and can be readily
mixed with water to obtain an instant refreshing drink.
96
 Fruit pulp can be combined with milk and sugar to form
milk shakes e.g. apple, mango, sapota etc.
Milk Beverages :
Milk beverages are prepared by the addition of different
flavours viz. strawberry, pista, cardamom and chocolate to milk.
They enhance the flavour of milk and thereby increase
its consumption particularly by young children. Milk beverages
are a good source of protein, calcium and vitamin A and B.
Milk shakes are prepared by mixing fruit pulp with milk
and sugar. Milk can be mixed with egg to prepare egg nog
which is a nourishing drink with a creamy consistency.
12.3 CARBONATED NON-ALCOHOLIC BEVERAGES
Carbonated non-alcoholic beverages are those beverages
that are generally sweetened, flavoured, acidified and coloured.
The chief ingredient is water and this may be to the
extent of 92 percent. The beverage contains 8 to 14 percent of
sugar which contributes to the sweetness, calorie and body of
the drink.
Artificial sweeteners such as saccharin is also used.
Carbon dioxide is added to produce the tingling effect, sparkle
and effervescence of carbonated beverages.
Nutritionally, it is an empty food since it provides only
calories and no other nutrients. A bottle of an aerated beverage
(180 ml) gives 70 kilo calories of energy.
Phosphoric acid, citric acid, fumaric acid and tartaric
acid which are added to enhance the flavour, makes the drink
acidic. It is therefore not recommended for patients suffering
from acidity and ulcers. Saccharin which is a suspected
carcinogen is also present in aerated drinks. Children should not
be encouraged to consume carbonated beverages since they have
no food value and depress the appetite.
12.4 MALTED BEVERAGES
Malted beverages are also known as Amylase Rich
Foods (ARF). This is prepared by steeping whole grain like ragi
or wheat in 2 – 3 times its volume of water.
97
 The excess water is drained and the moist seed is
germinated for 24 – 48 hours till sprouts appear. The grains are
sun-dried and roasted to remove moisture. Sprouts are removed
and the grains are milled and powdered.
The malt is cooked with water or milk to prepare a
nutritious beverage.
ARF is rich in enzyme amylase. The germination
process activates the enzymes of the resting grain and facilitates
the conversion of cereal starch to fermentable sugars.
Hydrolysis of cereal proteins also takes place.

12.5 TRADITIONAL BEVERAGES

Nera :
Nera is a sweet drink from the fresh sap of palm.
Tender Coconut Water :
This is a refreshing drink obtained from coconut.
Potassium, ascorbic acid and many vitamins of the B group are
present in coconut water. Apart from this, the water also
contains traces of calcium, phosphorus and iron.
Sugarcane juice :
Sugarcane juice extracted from sugarcane contains
12 – 15 percent sugar. The cane juice is acidic and in addition
to sugar contain minerals and vitamins such as the B – group.
Panakam :
Panakam is a traditional drink prepared using jaggery,
ginger and cardamom.

12.6 ALCOHOLIC BEVERAGES

Beer, ale, toddy, wine, whisky, gin and brandy are some
of the commonly used alcoholic beverages. Alcoholic
beverages are obtained by the enzymatic fermentation of yeast
which converts glucose to alcohol.
Beer :
Beer is made by fermenting barley malt extract with
yeast while ale is the same product with more stimulation.
98
Wine :
Wine is prepared by the fermentation of grapes with
yeast.
Toddy :
This is prepared by fermenting the sap of palm, coconut,
palymra or date palm.
Whisky :
It is an alcoholic distillate from a fermented mash of
grains.
Gin :
It is an artificial drink from diluted pure ethyl alcohol.
Brandy :
This is made by fermenting fruit juices like grapes and
apples.
Alcohol is a narcotic, a drug and sedative. It is very
rapidly absorbed in the body. Excessive consumption of alcohol
causes damage to the liver, stomach and blood vessels.
It also leads to diabetes mellitus, cardiovascular
diseases, cirrhosis of the liver and gastric ulcers.

12.7 SOUPS
Soups are prepared with vegetables, pulses, poultry and
meat. The food which is to be used for making soup is cooked
thoroughly in plenty of water. Clear soups are prepared using
only the water in which the food is cooked while cream soups
are prepared by adding milk and white sauce to the water.
Soups provide us with a variety of nutrients depending
on the ingredients used. Soups also enhance appetite and add
colour to the meal. It is usually served at the beginning of a
meal.

99
 QUESTIONS
Part-A
Fill in the blanks
1). To prepare good tea, _____________ teaspoonful of tea
leaves for 1 cup is ideal.
2). Coffee is classified as a ____________ beverage.
3). The protein content of cocoa is _______________.
4). Fruit squashes are prepared by combining _______ and
______.
5). The mineral present in significant amounts in milk beverages
is _______________.
6). Carbonated non-alcoholic beverage contain _______ percent
of sugar.
7).The expansion of ARF is _____________.
8). Nera is prepared from __________________.
9). Tender coconut water is a significant source of the mineral,
___________.
10). The pH of sugarcane juice is ____________.
11). Jaggery, ginger and cardamom are used in preparing a
traditional beverage called ________________.
12). In the preparation of alcoholic beverages _____________ is
converted to ___________ by the fermentation of
__________.
13). Beer is made by fermenting _____________.
14). The alcoholic distillate from a fermented mash of grain is
____________.

100
 Part- B
Write Short answers
1). Highlight the use of beverages in the day’ s menu.
2). Write a short note on the preparation of tea.
3). Describe the filtration and percolation method of preparing
coffee.
4). Compare the nutritive value of cocoa and chocolate.
5). Discuss the nutritional significance of fruit beverages and
soups.
6). How are malted beverages prepared? Highlights its
nutritional significance in the diet.
7).Write a short note on traditional beverages.

Part- C
Write detailed answers:
1). Differentiate alcoholic and non-alcoholic beverages. Discuss
different types of alcoholic beverages and highlight the
dangers involved in consuming excessive alcoholic
beverages?

2). What are the different types of non-alcoholic beverages?

Write a short note on any three of them. Highlight the use of
beverages in the day’ s menu.

3). What are the ingredients used in the preparation of

carbonated beverages? Would you recommend a fruit
beverage on a carbonated beverage for a teenager? Justify
your choice.

101
 13. FOOD PRESERVATION
Food is the basic necessity of man and is invaluable for
healthy existence. However, most foods fit for consumption
undergo deterioration and spoilage. In order to combat this
problem foods have to be preserved.
Food preservation can be defined as the science that deals
with the process of prevention of decay or spoilage of food thus
allowing it to be stored in a fit condition for future use.
Preservation of food increases the shelf life of foods and thus
ultimately ensures its supply during times of scarcity and natural
drought.

13.1 FOOD SPOILAGE

Food spoilage is a state in which food is deprived of its
good or effective qualities. Deterioration or spoilage starts from
the time food is harvested slaughtered or manufactured and
results in undesirable changes in the physical and chemical
characteristics of food.

Causes of food spoilage :

• Growth and activity of microorganisms such as bacteria,
yeast and moulds.
• Activities of food enzymes and other chemical reactions
within the food.
• Inappropriate temperatures for a given food.
• Gain or loss of moisture.
• Reaction with oxygen and light.
• Physical stress or abuse.
• Insects and rodents.
• Non-enzymatic reactions in food such as oxidation and
mechanical damage.
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13.2 PRINCIPLES OF FOOD PRESERVATION
1. Prevention or delay of microbial decomposition.
a) by keeping out micro organisms (asepsis)
b) by removal of micro organisms (eg. filtration)
c) by hindering the growth and activity of micro
organisms. (eg.) refrigeration, dehydration,
addition of chemical preservatives.
d) by killing micro organisms. (eg.) boiling,
irradiation.
2. Prevention or delay of self-decomposition of food.
a) by destruction or inactivation of enzymes. eg. by
blanching. The steaming or boiling of fruits or
vegetables in water for few minutes to inactivate
natural enzymes and facilitates removal of skin is
known as blanching.
b) by prevention or delay of purely chemical
reactions. eg. prevention of oxidation by the use of
anti-oxidants.
3. Prevention of damage caused by insects, animals and
mechanical causes.
13.3 METHODS OF FOOD PRESERVATION
A perusal of the history of food preservation reveals that
food preservation had its beginning from time immemorial and
could be traced to nearly a thousand years ago.
Salting of meat, fish, and vegetables was the oldest
method of preservation and could be traced back to the ancient
Egypt and Greek civilisations.
Pickling in salt and vinegar, sun-drying and preservation
of fruits and vegetables in sugar and honey were among the
other methods used.
Storage of food in frozen conditions was also practiced
for centuries in places where freezing temperatures were
recorded.

103
 The discovery of canning as a standard technique of
preserving foods in sealed containers subject to high
temperature was established in 1810 by Nicholas Appert.
Around 1860, Louis Pasteur discovered that microbes were the
main cause of spoilage and introduced a heat treatment known
as pasteurisation to the world.
All methods used for food preservation are based on
preventing or retarding the cause of spoilage.
When growth of micro organism is only retarded,
preservation is temporary. When spoilage organisms are
completely destroyed a more permanent preservation is
achieved.

13.3.1 Use Of Low And High Temperatures :

Use of Low Temperatures
Microbial growth and enzyme reaction are retarded in foods
stored at low temperatures.
The lower the temperature, the greater the retardation. The
low temperatures employed can be:
1. Cellar storage temperature (about 15º C)
2. Refrigerator or chilling temperature (0º C to 5º C).
3. Freezing temperature (-18º C to -40º C).
Cellar storage temperatures (about 15º C):
Temperatures in cellars (under ground rooms) where
surplus food is stored in many villages are usually not much
below that of the outside air and is seldom lower than 15º C.
The temperature is not low enough to prevent the action
of many spoilage organisms or of the plant enzymes.
Decomposition is however, slowed down considerably.
Root crops, potatoes, onions, apples and similar foods
can be stored for limited periods during the winter months.
Refrigerator or Chilling Temperature (0º C to 5º C)
Chilling (refrigerator), temperatures are obtained and
maintained by means of ice or mechanical refrigeration.
104
 Fruits and vegetables, meats, poultry, fresh milk and
milk products, fish and eggs can be preserved from two days to
a week when held at this temperature.
In addition to the foods mentioned above, foods prepared
for serving or left-overs may also be stored in the household
refrigerator. The best storage temperature for many foods, eggs,
for example, is slightly above 0º C.
The optimum temperature of storage varies with the
product and is fairly specific for any given food.
Besides temperature, the relative humidity and the
composition of the atmosphere can affect the preservation of the
food.
Commercial cold storages with proper ventilation and
automatic control of temperatures are now used throughout the
country (mostly in cities) for the storage of semi perishable
products such as potatoes and apples. This has made such foods
available throughout the year and has also stabilized their prices
in these cities.
Low temperatures chiefly inhibit the growth of
microorganisms although freezing may result in the destruction
of some microorganisms.
Freezing temperature:
Freezing may preserve foods for long periods of time
provided the quality of the food is good to begin with and the
temperature of storage is far below the actual freezing
temperature of food.
Some microorganisms are destroyed during freezing
preservation. The chief preservative effect of freezing
temperatures lies in the inability of microorganisms to grow at
freezing temperature.
In vegetables, enzyme action may still produce
undesirable effects on flavour and texture during freezing. The
enzymes therefore must be destroyed by heating before the
vegetables are frozen.

105
Slow freezing process
It is also known as sharp freezing. In this method, the
foods are placed in refrigerated cabinets at temperatures ranging
from -4º C to -29º C. This method is adopted in home-freezers.
Freezing may require from 3 to 72 hours under such conditions.

Quick freezing process

The lower temperatures used -32º C to -40º C freeze foods
so rapidly that fine crystals are formed and the time of freezing
is greatly reduced over that required in sharp freezing.
The fine crystals formed by quick freezing have a lesser
effect on breaking up plant and animal cells than do methods of
slow freezing that produce coarser ice crystals.
In quick freezing, large quantity of food can be frozen in
a short period of time.

Dehydro freezing
Dehydro freezing of fruits and vegetables consists of
drying the food to about 50 percent of its original weight and
volume and then freezing the food to preserve it.

The quality of dehydro frozen fruits and vegetables is

equal to that of fruits and vegetables frozen without preliminary
drying.

The cost in packing, freezing, storing and shipping of

such foods is less because of the reduction in weight and volume
of foods during dehydro-freezing .

The following figure depicts the relationship between

bacterial growth and temperature. The rapid growth of bacteria
occurs in the temperature zone of 60° F –120° F

106
 Fig.13.1 Temperature in relation to bacterial growth
Source : Srilakshmi. B (2003) Food Science, New Age
International Publishers, Chennai.
107
Use of heat or high temperatures:
The destruction of microorganisms by heat is due to the
coagulation of the protoplasm. The temperature and time used in
heat processing a food depend upon the nature of the food and
what other methods are combined with heat.
The various degrees of heating used in preservation of food
can be classified into three (a) Pasteurisation, (b) Heating upto
100º C or 212º F and (c) Heating above 100º C.
(a) Pasteurization
The time and temperature used in the pasteurization
process depend upon the product treated and the method used. In
pasteurization, most of the spoilage organisms are killed but a
few survive and hence must be inhibited by low temperatures or
some other method, if spoilage is to be prevented.
There are two methods of pasteurization – flash method
and holder method. In flash method, otherwise called high-
temperature short time method, a high temperature for a short
time is used, while in the holder method or low-temperature
long-time method, a lower temperature for a longer time is used.
There are slight variations in the time and temperature used for
pasteurizing different foods, like milk, cream, ice cream mix and
wines.
(b) Heating up to a temperature of about 100º C
Most methods of cooking come under this. This
temperature can be obtained by boiling any liquid food, by
immersing a container in boiling water or by exposure to steam.
Before the use of pressure cookers and autoclaves, canning was
done at 100º C and this killed all bacteria except spores.
(c) Heating above 100º C(212º F)
Temperatures above 100º C are obtained by means of
steam under pressure as in a pressure cooker or autoclave.
Sterlisation of foods can be brought about at 121º C for 15
minutes under moist conditions.

108
CANNING PROCEDURE
The details of canning procedures vary with the nature of the
food to be canned, but there are certain important operations
common to canning of all foods.
(1) Cleaning (2) Blanching (3) Exhausting, (4) Sealing the
container (5) Sterilizing the sealed container and
(6) Cooling the container
1. Cleaning :
The first step in canning, whether done in the home or on
a large scale in factories, is the thorough cleaning of the raw
food to be preserved. By this means most of spoilage organisms
are removed.
On a large scale, cleaning is done with the help of
various kinds of washers. The raw materials may be subjected to
high pressure sprays or strong flowing streams of water, while
passing along a moving belt.

2. Blanching:
Blanching consists of the immersion of raw food materials,
especially vegetables and fruits, into hot water or exposure to
live steam.
Blanching serves as an additional hot water wash. It
softens fibrous plant tissues, inhibits the action of enzymes and
fixes the natural colour of certain products making them more
attractive in appearance.

3. Exhausting:
Gases are expelled by passing the open can containing
the food through an exhaust box in which hot water or steam is
used to expand the food and expel air and other gases from the
contents and the head space area of the can. After the gases are
expelled, the can is immediately sealed, heat processed and
cooled.
In the case of certain products, exhausting is done by
mechanical means, rather than by the use of heat. There are
109
special machines which withdraw the air from the cans and they
seal them at the same time – “vacuum packing”.
4. Sealing the container:
Each container must be sealed properly before it is
subjected to the heat process, since re-contamination of the
contents must be prevented.
5. Sterilizing the sealed container with its contents by heat
processing
This is meant to bring about complete sterilisation to
prevent spoilage of the food by microorganisms. This is usually
done by the application of steam under pressure.
The temperature and time used for heat processing
depend on the kind of food, on the pH of the medium and other
factors. It should however be remembered that an excessive
period of heating at higher temperatures than necessary will
spoil the product.
A longer exposure to a relatively low temperature should
be preferred to a short exposure at a higher temperature.
6. Cooling the container
The containers should be cooled rapidly to check the action of
heat and prevent unnecessary softening of the food or change in
colour of the contents. Cooling can be done by means of air or
water.
13.3.2 Drying :
Microorganisms need moisture to grow. When exposed
to sunlight or subjected to dehydration, the moisture in the food
is removed and the concentration of water is brought below a
certain level. This prevents the growth of microorganisms and
thereby spoilage of food.
Food preservation by drying is one of the oldest methods
practised from ancient times. This method consists of exposing
food to sunlight and air until the product is dry.
Treatment of foods before drying
• Selection and sorting for size, maturity and
wholesomeness.
110
 • Washing, especially of fruits and vegetables.
• Peeling of fruits and vegetables by hand, machine or
abrasion.
• Subdivision into halves, slices, shreds or cubes.
• Blanching or scalding of vegetables and some fruits like
apricots and peaches.
• Sulphuring of light coloured fruits and vegetables. Fruits
are sulphured by exposure to sulphur di oxide gas
produced by the burning of sulphur to a level of 1,000 to
3,000 ppm.

Methods Of Drying
Sun drying
It is limited to regions with hot climates and a dry
atmosphere and to certain fruits such as raisins, prunes, figs,
apricots, pears and peaches. It is a slow process.
Many Indian foods are preserved by sun drying. Papads
and vathals are made using this principle. Vegetables like cluster
beans and curd chilli and fruits like jack fruit are preserved by
this method. Fish and meat are also sun dried.
Drying by mechanical driers
Most methods of artificial drying involve the
passage of heated air with controlled relative humidity over the
food to be dried or the passage of the food through such air.
Fruits, vegetables, nuts, fish and meat can be
successfully preserved by this method. In the dehydration
process, artificial drying methods (eg. spray drier) are used for
drying foods. Although it is expensive when compared to
natural sun-drying procedures, it is very advantageous because
the temperature and relative humidity can be manipulated.
Spray drying
Milk and egg are dried to a powder in spray driers in
which the liquid is atomized and sprayed into a hot air stream
for almost instant drying.

111
Foam mat drying
Foam mat drying may be used commercially with orange
and tomato juice. In this process a small amount of edible foam
stabilizer such as monoglycerides or a modified soyabean
protein with methyl cellulose is added to the liquid and a stiff
foam is produced by whipping. The foam is spread in a thin
layer and dried in a stream of hot air. The product separates
easily into small particles on cooling.
Drying by osmosis
Drying also results when fish is heavily salted. In this
case, the moisture is drawn out from all cell tissues. The water is
then bound with the solute, making it unavailable to the
microorganisms.
In osmotic dehydration of fruits, the method involves the
partial dehydration of fruits by osmosis in a concentrated sugar
solution or syrup.
Freeze drying
Removal of water from a product while it is frozen by
sublimations is called freeze drying.
Factors to be considered in drying foods
• The temperature employed, which will vary with the
food and the method of drying.
• The relative humidity of the air. It usually is higher at the
start of drying than later.
• The velocity of the air.
• The duration of drying.
13.3.3 Use Of High Concentration Of Sugar And Salt :
Sugar and salt aid in the preservation of products in
which it is used due to their ability to bind water and make it
unavailable for microbial growth. Salt is an effective
preservative because it also ionises to yield chlorine ion, which
is harmful to organisms and reduces the solubility of oxygen in
moisture, which are essential for the growth, and multiplication
of microorganisms.
112
 Jams, jellies and fruit juices are an important class of
fruit products preserved using high concentration of sugar.
Pickles are preserved using high concentration of salt.
Jam :
Jams are prepared by boiling fruit pulp with sufficient
amount of sugar to a reasonably thick consistency, firm enough
to hold the fruit tissue in position. In preparing jam, the fruit is
crushed or finely cut and measured quantity of sugar and
preservatives are added so that when cooked, the mass is fairly
uniform throughout.
Jams can be prepared from all varieties of pulpy fruits
such as grapes, mango, sapota, banana, guava etc.
Jelly :
Jellies are prepared by boiling fruits in water. The
extract obtained is strained and measured quantity of sugar is
added to it. The mixture is then boiled to a stage at which it will
set to a clear gel. A perfect jelly should be transparent, well set,
but not too stiff and should have the original flavour of the fruit.
It should retain its shape when removed from the mould.
Usually fruits such as guava, pineapple, apple, grape and a
mixture of fruits rich in pectin can be used for the preparation of
jellies.
Fruit Juices :
Fruit beverages are prepared from different fruits such as
apple, mango, grapes, lime, pineapple, sapota and in different
forms such as pure juices, crushes, squashes and cordials.
The ratio of sugar and fruit juice in the preparation of
various beverages are as follows :
Crushes - 25% fruit juice and 55% sugar.
Squashes - 25% fruit juice and 45% sugar.
Cordial - Clarified juice 1 litre and 250 gm sugar.
In the preparation of fruit juices, citric acid is usually
added to clarify the sugar syrup. Preservatives such as sodium
benzoate are added to tomato and grape juices while potassium
meta bi-sulphite (KMS) is added to all other fruit beverages.
113
Pickling :
The preservation of fruits and vegetables in common
salt, vinegar, oil and spices is referred to as pickling. Salt binds
the moisture in the food and thereby prevents the growth of
microorganisms.
The layer of oil that floats on the top of pickles prevents
the entry and growth of microorganisms like moulds and yeast.
Spices, like turmeric, pepper, chilli powder and asafoetida retard
the growth of bacteria.
Vinegar lowers the pH of the product thereby providing
an unfavourable acidic environment for microbial growth.
Mango, lime, ginger, garlic, tomato, chilli, mixed vegetables
such as beans, carrot, cauliflower and peas are used widely in
the preparation of pickles.

13.3.4 Use Of Chemical Preservatives

Preservatives are defined as chemical agents which serve
to retard, hinder or mask the undesirable changes in food. These
changes may be caused by micro organisms, by enzymes of food
or by purely chemical reactions.
Certain chemicals when added in small quantities can
hinder undesirable chemical reaction in food by:
1. Interfering with the cell membrane of the
microorganism, their enzyme activity or their genetic
mechanism.
2. Acting as antioxidants.
Maximum amounts allowed to be added to each type of
food is regulated by law because higher concentrations can be a
health hazard. Benzoic acid in the form of its sodium salt is an
effective inhibitor of moulds and is used extensively for the
preservation of jams and jellies.

Some of the other chemical preservatives used are:

1. Potassium metabisulphite
2. Sorbic acid
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 3. Calcium propionate
4. Sodium benzoate
The development of off-flavours (rancidity) in edible oils
is prevented by the use of Butylated Hydroxy Anisole(BHA),
Butylated Hydroxy Toluene(BHT), lecithin which are some of
the approved antioxidants.

13.3.5 Radiation
Radiant energy can be used to preserve food. Gamma
rays and beta particles produced by special electronic machines
are sources of energy used to preserve food.
These waves penetrate throughout the food. As the
waves and particles pass through the food, they collide with
molecules in the food and in microorganisms. These result in
chemical alterations. The goal of irradiation is to kill the
microorganism and inactivate the enzymes without altering the
food.
Changes in the food are minimized if it is done in a
vacuum, and if ascorbic acid is present. Berries and meat are
preserved in this way.

Questions

Part- A
Fill in the blanks :
1. Keeping out of microorganism is known as
____________.
2. Low temperatures ________________the growth of
microorganisms.
3. High temperatures ________________ the growth of
microorganisms.
4. Artificial drying is known as _____________.
5. Jam is prepared using fruit ___________ while jelly is
prepared using fruit __________.

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 6. The preservation of fruits and vegetables using salt,
vinegar, oil and spices is known as _______________.
7. Fruit squashes contain _________% sugar and
________% fruit juice.
8. During the canning process, fruits and vegetables are
blanched in order to ________ and _________.

Part - B
Write short answers
1. Define preservation. What are the principles of food
preservation?
2. What are the causes of food spoilage?
3. How does salt and sugar lend themselves as
preservatives?
4. Differentiate jams and jellies.
5. What are the functions of the ingredients used in
pickling?
6. Write a note on chemical preservatives and its use?

Part- C
Write detailed answers
1. How do low temperature procedures prevent spoilage?
Discuss the methods.
2. Give a brief account on any four techniques employed
in food preservation.
3. Explain the use of high temperature in preservation?
4. Define Canning. Explain the steps in canning?
5. Give a detailed account on drying as a method of
preservation. Write a note on the different types of
drying?

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 14. FOOD ADULTERATION

14.1 DEFINITION
Adulteration is defined as the process by which the quality
or the nature of a given substance is reduced through the
addition of a foreign or an inferior substance and the removal of
a vital element.
14.2 TYPES OF ADULTERANTS

Adulterants

Intentional Incidental

Intentional :
Intentional adulterants are those substances that are
added as a deliberate act on the part of the adulterer with the
intention to increase the margin of profit. Eg. sand, marble
chips, stones, mud, chalk powder, water, dyes, etc., These
adulterants cause harmful effects on the body.
Incidental :
These adulterants are found in food substances due to
ignorance, negligence or lack of proper facilities. It is not a
willful act on the part of the adulterer. Eg. pesticides, droppings
of rodents, larvae in food.

14.3 TESTS FOR DETECTING ADULTERANTS

Simple tests for the detection of adulterants present in
foods are given in the following table :
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 TABLE 14.A
Tests for determining common adulterants present in food

Sl. Substance Adulterant Test

No.
1. Asafoetida Resin or a) Pure asafoetida
(Hing) gum dissolves in water
scented and to form a milk-
coloured white solution.
b) Pure asafoetida
burns with a bright
flame on being
ignited (burning)
2. Sugar Chalk Dissolve in a glass of
Powder water. Chalk will settle
down in the bottom.
3. Cardamom Oil is On rubbing, talcum will
removed stick to the fingers. On
and pods testing, if there is
are coated hardly any aromatic
with talcum flavour, it indicates
powder. removal of essential oil.
4. Turmeric Metanil When concentrated
(Haldi) Yellow hydrochloric acid is
colouring added to a solution of
turmeric powder, it
turns magenta if
metanil yellow is
present.
5. Chilli Sawdust Sprinkle on the surface
powder and colour of water, sawdust
floats. Added colour
will make the water
coloured.

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6. Coffee Chicory Shake a small portion
in cold water. Coffee
will float while chicory
will sink, making the
water brown.
7. Coriander Horse dung Soak in water. Horse
powder powdered dung will float which
can be easily detected.
8. Cloves(Lav Oil may be If so, cloves may be
ang) removed shrunken in appearance.
9. Cumin May If rubbed in hand,
seeds contain fingers will turn black.
(Jeera) grass seeds
coloured
with
charcoal
dust
10. Ghee Vanaspathi Dissolve one teaspoon
of sugar in 10 ccs of
hydrochloric acid and
10 ccs of the melted
ghee and shake
thoroughly for one
minute. Allow it to
stand for 10 minutes. If
vanaspathi has been
added, the aqueous
layer will be red in
colour.
11. Jaggery Metanil Hydrochloric acid
yellow added to a solution of
jaggery will turn its
colour to magenta.

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12. Rawa Iron filing Pass magnet through
to add the rawa. Iron filings
weight will cling to it.
13. Betelnut Sawdust Sprinkle in water.
powder and Sawdust will float and
(Supari) artificial the added colour will
colour dissolve in water.
14. Milk a. Mashed Add a drop of tincture
potato, of iodine. Iodine, which
other is brown turns blue if
starches starch is present
b. Water Put a drop of milk on a
polished vertical
surface and allow to
flow. Pure milk flows
slowly leaving a white
trail. Adulterated milk
will flow immediately
without leaving a mark.
15. Tea dust Used tea Sprinkle the dust on a
leaves wet white filter paper.
dried, Spots of yellow, pink
powdered and red appearing on
and the paper indicates that
artificially tea is artificially
coloured. coloured.
16. Edible oil Argemone A reddish-brown
precipitate is formed
when oil and
hydrochloric acid are
gently mixed with ferric
chloride solution, if
argemone is present.
17. Saffron Maize a) Genuine saffron is
fibres dried, tough. Spurious
coloured120 saffron is brittle and
and scented breaks easily.
 fibres dried, tough. Spurious
coloured saffron is brittle and
and scented breaks easily.
b) Dissolves easily in
water, giving aroma
of saffron.
18. Sago Sand and Gritty feel in mouth.
talcum Pure sago swells on
burning and leaves
hardly any ash.
19. Black Dried seeds Papaya seeds are
pepper of papaya shrunken oval in shape
fruit and greenish brown in
colour and has a
repulsive flavour
distinct from the bite of
black pepper.
20. Coconut oil Any other Place a small bottle of
oil oil in refrigerator.
Coconut oil solidifies
leaving the adulterant
as a separate layer.
21. Bajra Fungus Immerse in salt water.
(Ergot Fungi will float to the
infested) top.
22. Cinnamon Cassia bark Added colour comes off
(Dalchini) in water.
23. Common White Stir a spoonful of
salt powdered simple salt in a glass of
stone, chalk water. The presence of
chalk will make the
solution white
24. Honey Molasses A cotton wick dipped in
( sugar and pure honey when
water) lighted with a match
121 stick burns, if
adulterated it will not
burn and will produce a
 water) lighted with a match
stick burns, if
adulterated it will not
burn and will produce a
cracking sound.
25. Peanut oil Cottonseed Mix 2.5 ml of oil or fat
oil with 2.5 ml Halphen’ s
reagent. Lightly screw
cap and heat in boiling
water for 30 minutes.
The test is positive if a
rose colour is obtained.
14.4 PACKAGING MATERIALS AND HAZARDS
Materials used for packing
The conventional methods of packing which are prevalent even
now to a large extent are tin or aluminium containers, glass
bottles and jars, paper and waxed paper wrappings, paper
cartons, cardboard and certain plastic containers. Tin and
aluminium containers have become costly and glass bottles
though very good in many respects have problems associated
with breakage and heavy transportation charges on account of
weight. Continued use of paper in increased volume dwindles
the natural resources.
Against the conventional materials there has emerged
increased usage of newer materials derived synthetically. Some
polymeric plastic materials are polystyrene, polyvinyls,
polyvinydines and derivatives, vinyl acetate, poly ethylene,
polypropylene and polyesters.
Folding cartons and paper board boxes are used
extensively in the food industry. Tin plate containers—the
cylindrical open-top variety are mostly used for processed foods.
Aluminium is used principally as foil e.g., chocolates. It is also
used as bottle caps and closures and easy open tops for cans.

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 Polystyrene is principally made into tubs for ice creams,
packs for eggs, sausages and small packages for butter, jam and
cheese. Bags made from the simplest of all plastic polymers,
namely, polyethylene or ‘ polythene’ as commonly known have
relatively low preserving qualities. Material such as polyesters
vinyl acetate derivatives and multilayer films made out of a
combination of different materials have good preserving
characteristics for food products.
Timber crates are used extensively for packing weights
above 100 kg. Plastic crates are well established in the dairy
industry and for the transportation of bottled beer, mineral
water and soft drinks. High density polythene is used for milk
crates.
Shrink wrap packaging is a system where heat shrinkable
thermoplastic film is wrapped around an article or a group of
articles. The film is made to shrink around it by the application
of heat to achieve a skin light package. Canned food products,
bottles and jars of all types can be shrink wrapped.
Now-a-days it is expected that packaging material be
environment friendly or ecofriendly, that is, it should not pose
many problems for mankind and hazards to the environment.
For example, corrugated boxes are ecofriendly and are preferred
for exporting. They can be effectively replaced for conventional
wooden boxes which need to destroy the trees. Recyclability of
packaging is desirable so as to preserve the resources of the
packaging material for future generations.
Packaging Hazards:
Plastics such as cellulose acetate, polyamide polyethylene
polypropylene and polyvinyl chloride are often used as packing
materials because they are light in weight and are resistant to
diffusion due to solvents and high temperatures.
However care should be taken that only food grade plastic
packing materials should be used for packaging foods to prevent
the following packaging hazards.

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 1. Production of noxious thermal breakdown products
which are injurious to health.
2. Formation of toxic residues that result when subjected to
heat treatment for sterilisation of the contents.
3. Unfavourable reactions between acid and oil content of
the food and the packaging material.
14.5 FOOD LAWS AND STANDARDS
Prevention of Food Adulteration Act.
The prevention of Food Adulteration Act, (PFA) 1954
operated by the Directorate General of Health Services, Ministry
of Health was designed for the following purposes :
1. It formulates and monitors the standard of quality and purity
of foods with emphasis on prevention of adulteration of
foods.
2. It is the basic structure intended to protect the common
consumer against the supply of adulterated foods.
3. It makes provision for prevention of adulteration of food and
lays down the rule that no person shall manufacture for sale,
store, sell or distribute any adulterated or misbranded food
or food which contravenes the provision of act or rules.
4. It has set the yardstick to ascertain adulteration. According
to this act, a food is deemed to be adulterated – if:
Ø It is not of the nature, substance and quality, which the food
ought to be.
Ø It contains any other substance which affects, or if the article
is so processed so as to affect injuriously the nature,
substance and quality of the food.
Ø It contains added inferior or cheaper substance that affects
the nature and quality of the food.
Ø Any constituent of the food is removed so as to affect
injuriously the nature, quality and substance of the food.
Ø It is prepared, packed and stored under unsanitary
conditions.

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Ø It contains any filthy, disgusting, rotten, decomposed
substance of a diseased animal or vegetable substance or is
insect-infested or otherwise unfit for human consumption.
Ø The article is obtained from a diseased animal.
Ø The article contains a poisonous ingredient or any other
ingredient injurious to health.
Ø The container renders the food injurious to health.
Ø It contains excessive or prohibited colours.
Ø It contains excessive or prohibited preservatives.
Ø It does not satisfy the standards prescribed by the authorities.
Under the provision of the PFA Act, the Government of
India has promulgated PFA rules which specifies the following
details:
1. Qualification, duties and functions of food analysts, food
inspectors and central food laboratory.
2. Procedure for drawing test samples and sending them to
the analyst and laboratory.
3. Specification for the identity and purity of food.
4. Tolerance for contaminants, preservatives, emulsifiers
and other additives.
Agmark Standard:
The word Agmark is derived from the words
‘ Agricultural Marketing’ . It is a standard of quality based on the
physical and chemical characteristics of food, both the natural
and those acquired during processing.
Products graded under AGMARK include vegetable oils,
ghee, butter, rice, groundnut, pulses and spices. These standards
ensure accurate weight and correct selling price.
Bureau of Indian Standards :
The Bureau of Indian Standards lays down criteria for
standardisation of vegetables and fruit products, spices and
condiments, animal products and processed food.
Manufacturers are allowed to use the BIS label on each
unit of their product, if their products conform with the
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standards laid down by BIS. The products are checked for
quality by laboratories certified by BIS. BIS is also known as
ISI (Indian Standard Institution).
Some of the items which require compulsory BIS
certification under PFA Act include artificial food colours,
natural food colours, food additives, infant formula, milk-cereal
based weaning foods, milk powder and condensed milk.
Questions
Part- A
Fill in the blanks:
1. Adulterants that are added deliberately are known as
________ adulterants.
2. The adulterant found in ghee is ________
3. Milk is usually adulterated with ___________ and
_______
4. The word Agmark is derived from the words ________
Part- B
Write short Answers
1. Define adulteration. What are the type of adulterants ?
Differentiate them.
2. List the adulterants found in the following items. Suggest
simple methods for detecting the adulterants –
a) Milk b) Tea c) Sugar d) Honey
3. What is BIS ? Explain its use.
Part-C
Give detailed Answers
1. Under what condition is a food deemed as adulterated
according to the PFA Act, 1954. What is the role of the PFA
Act ?
2. List at least 10 articles normally adulterated. Name the
adulterant and the test for detection.
3. Give a detailed account on food laws and standards.
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 15. DEVELOPMENT IN FOOD
TECHNOLOGY

15.1 FOOD TECHNOLOGY – GENETICALLY

MODIFIED FOODS
Bio-technology centres around the microbes and cells
taken from plants and animals and their ability to synthesize wide
range of valuable substances.
The important area of bio-technology application is the
qualitative improvement in foods.
The principle governing genetic engineering is that
genetic material which is also known as DNA can be transferred
from a cell of one species to another unrelated species to express
itself in the recipient cells. This is also known as recombinant
DNA technology.
Foods modified through the transfer of genes are known
as Genetically Modified Foods (GM Foods).

Advantages of GM Technology :
1. It is much faster and cheaper and allows a greater
precision in selecting desirable characteristics when
compared to traditional breeding techniques.
2. It gives rise to pest and virus resistant crops.
3. Nutritional improvement-Genes that control desired micro
nutrients can be transferred to obtain new crops with
increased vitamin and mineral content.
The introduction of genetically modified crops
with increased vitamin and mineral content is of great
importance owing to the prevalence of nutrient
deficiencies around the world. Iron - rich rice, quality
protein-maize, high carotene-sweet potato and micro
nutrient rich seeds are some of the outcomes of research
in food bio-technology.

127
 In our country genetically modified rice, potatoes and
tomatoes are under experimentation. The golden rice with
enhancement of vitamin A is an example.
4. Adaptive to harsh conditions – Genetic modification
enables crops to grow in harsh conditions like drought
and temperature extremes.
15.2 NEUTRACEUTICALS
The word neutraceuticals originates from the word
`nutrition’ and `pharmaceuticals’ . It implies the usage of food as
protective drugs or as food supplements.
Food stuffs contain disease preventing phytonutrients.
These include terpenes, phytosterols, phenols and theols.
Terpenes :
Terpenes are found in green foods, soya products and
grains. Carotenoids and limonoids are the subclass of terpenes.
Carrot, tomato, parsley and spinach are rich sources of
carotenoids. They are a precursor of vitamin A and also prevent
eye diseases.
Limonoids is present mainly in citrus peels and it is an
anti-oxidant. It helps in protecting the lung tissue.
Phytosterols :
Yellow vegetables, yam and the seeds of pumpkin are a
rich source of phytosterols. They help in the excretion of
cholesterol and prevent tumors in the prostrate gland and breast.
Phenols :
They play an important role in preventive medicine. They
prevent the damage of tissues and inflammation. Berries, grapes
and brinjals are good sources of phenols. Flavonids,
anthocyanidines and isoflavones are important sub classes of
phenols.
Flavonoids enhance the effectiveness of vitamin C,
prevent allergies, tumors, platelet aggregation and reduce chance
of oestrogen induced cancers.
Anthocyanidines help in the synthesis of protein collagen
while isoflavones present in bean, legumes and soyabeans
prevent tumors, breast and prostrate cancer.
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Theol :
They are the sulphur containing class of phytonutrients.
Garlic, onion, cabbage and turnip contain theols. Garlic and
onion contain allyl sulphides which are anti-carcinogenic agents.
They protect against tumors and prevent cardio-vascular
disorders. Cabbage and turnip are known to reduce tumors.

15.3 ALGAE AS FOOD

Blue green algae, Spirulina fusiformis is used as nutrient
dense food. The major protein in spirulina is phycocyanin which
is a deep blue colour pigment and about 150 mgs. It is present in
1 gm of spirulina.
Spray dried spirulina is rich in protein, vitamins
particularly -carotene and gamma linolenic acid. One gram
spirulina contains carotenoids equivalent to 1 kg of vegetables
and yellow fruits.
Gamma linolenic acid of spirulina help in heart disease,
premenstrual stress, obesity and arthritis. The composition of
spirulina is given in the following table
TABLE 15A
Composition of spray dried spirulina per 100g
Nutrient Amount
Energy Kcal 346
Carbohydrate g 16
Protein g 65 - 71
Fat g 6.7
Calcium mg 658
Iron mg 47.7
B - Carotene µg 3,20,000
Tocopherol IU 0.73
Folic acid µg 176

Source: Srilakshmi.B (2003) Food Science, New Age

International (P) Publishers Ltd, Chennai.

129
 The quality of protein of spirulina is better than cereals and
soya proteins. One serving of spirulina is better than one serving
of egg or milk in nutritive value.

Spirulina apart from being rich in nutrients contains

phytonutrients like gamma linolenic acid, sulpholipids,
phycocyanin and antioxidant vitamins.

The pigments present in Spirulina can raise the activity

of lymphocytes, increase immunity and prevent certain type of
cancers.
15.4 ORGANIC FOODS
Organic foods are environment friendly foods. These are
foods produced without artificial fertilizers or pesticides. Animal
manure and compost are used as natural fertiliser and the system
of crop rotation further enriches the soil.
Pesticides disturb ecological balance. In the place of
pesticides, particular insects and fungi are used to control specific
pests.

15.5 FUNCTIONAL FOODS

A functional food is any food that has a positive effect on
health, physical performance or state of mind beyond the benefit
of nutrition.
Foods rich in antioxidants (β - Carotene, Vitamin C and
E) protect the body from the detrimental effect of free radicals
that cause coronary heart diseases and cancer. β - Carotene is
present in green leafy vegetables and yellow-orange coloured
fruits and vegetables.
Vegetable oils, dark green leafy vegetables, nuts and
whole grains are a good source of vitamin E and help to maintain
the integrity of cells and reduce thrombus formation. Citrus
fruits, guava and vegetables such as cabbage and drumstick
leaves are a good source of Vitamin C.

130
 Hypocholesterolemic agents such as garlic, fenugreek
soya protein, guargum in cluster beans and phytochemicals
(pigments and flavouring substances in fruits and vegetables) are
functional foods as they protect from heart diseases and cancer.
.Herbs and spices such as black pepper, thyme, turmeric
have shown to possess anti-oxidant property and are therefore
known as functional foods.

Questions

Part A
Fill in the blanks
1. Foods modified through the transfer of genes are known
as _________
2. ________ and ________ are a subclass of terpenes
3. _______, _______, ______ Foods produced without the
use of artificial fertilizers and pesticides are known as
__________ foods.
4. A ___________ is any food that has a positive effect on a
persons health beyond the benefit of nutrition.

Part B
Write Short answers:
1. Explain the role of genetically modified foods in the
prevention of micro nutrient deficiencies.
2. Give the advantages of bio-fortification.
3. What are neutraceuticals ? Explain the benefits of using
them.
4. Write a note on algae as food.
5. What are functional foods and organic foods

Part C
Write detailed answers:
Give a detailed account on the recent trends in food
technology
131
 16. INTRODUCTION TO NUTRITION
SCIENCE
Nature has provided a variety of foods for man to
consume and be healthy. We consume food for maintenance of
health, growth and to develop greater resistance against
infections.
Foods contain substances called nutrients in varying
proportions, which are needed for proper growth and
maintenance of life processes. Knowledge of the functions of
these nutrients and major food sources is necessary for man to
formulate a nutritious diet.

16.1 DEFINITION AND HISTORY OF NUTRITION

Nutrition is defined as a science concerned with the role
of food and nutrients in the maintenance of health.
Nutrition as defined by Robinson (1982) is “ the science
of foods and nutrients, their action, interaction and balance in
relationship to health and disease, the processes by which the
organism ingests, digests, absorbs, transports and utilizes
nutrients and disposes of their end product”.
Nutrients are the constituents in food that must be
supplied to the body in adequate amounts. These include
Carbohydrates, Proteins, Fats, Minerals and Vitamins.
Nutritional status is the condition of health of the individual as
influenced by the utilization of the nutrients.
The science of Nutrition has been developed by using the
combined knowledge of the physical and biological sciences. Its
application involves the social sciences related to man’ s
behaviour - Psychology, sociology, anthropology and
economics.
Until World War I the significance of nutrition was
recognized by a relatively small group of scientists and
132
physicians. Since then, a wider awareness has developed on the
role of nutrients in health of individuals and the economic
development of the nation.
A great number of important discoveries and
developments in this field have enabled health care
professionals to understand the nutrient needs of people and the
means of supplying them. It is difficult to set in a chronological
order of events that show the development of nutrition.
Many aspects developed simultaneously or overlapped
each other. Some discoveries went unnoticed for several years
because scientific attention was occupied with other
developments and theories.
Some progresses were stimulated by national
emergencies. Others depended on technical development of the
supporting sciences. Nutrition research in India, as beri – beri
inquiry was started in 1918, under the guidance of Sir Mc
Carrison at Coonoor in South India.
It has blossomed into an important national institution, at
Hyderobad called National Institute of Nutrition. It is currently
engaged in carrying out basic as well as applied research work
in nutrition. This national institute comes under the Indian
Council of Medical Research (ICMR).
16.2 RELATION BETWEEN GOOD NUTRITION AND
HEALTH
Health is defined by the World Health Organization
(WHO) as the “ State of complete physical, mental and social
well being and not merely the absence of disease or infirmity”.
To maintain good health and nutritional status one must
eat a balanced food, which contains, all the nutrients in the
correct proportion.
The essential requisites of health would include the
following:
1. Achievement of optimal growth and development,
reflecting the full expression of one’ s genetic potential.
133
 2. Maintenance of the structural integrity and functional
efficiency of body tissues necessary for an active and
productive use.
3. Mental well-being
4. Ability to withstand the inevitable process of aging with
minimal disability and functional impairment.
5. Ability to combat diseases such as
– resisting infections (immunocompetence)
– preventing the onset of degenerative diseases
– resisting the effect of environmental toxins/ pollutants
Till three decades ago the role of nutrition in growth and
development and tissue integrity alone was clear, but now the
persuasive role nutrition plays in the other dimensions of health
is implicit. Hence an optimal nutritional status is an indication of
good health. This recent advance has brought about a large-scale
change in dietary habits and practices of the population.

16.3 CONCEPTS OF MALNUTRITION – UNDER

NUTRITION AND OVER NUTRITION
Malnutrition as defined by World Health Organisation
(WHO) is a pathological state resulting from a relative or
absolute deficiency or excess of one or more essential nutrients,
this state being clinically manifested or detected only by
biochemical, anthropometric or physiological tests.
Four forms can be distinguished:
a. Undernutrition – the pathological state resulting from the
consumption of an inadequate quantity of food over an
extended period of time.
b. Marasmus is synonymous with severe undernutrition.
Starvation implies total elimination of food and hence
the rapid development of under nutrition and marasmus.
c. Specific deficiency – the pathological state resulting
from a relative or absolute lack of an individual nutrient.
d. Over nutrition – the pathological state resulting from a
disproportion of essential nutrients with or without the
134
 absolute deficiency of any nutrient as determined by the
requirement of a balanced diet.

16.4 SIGNS OF A WELL NOURISHED CHILD AS

AGAINST THOSE OF AN ILL NOURISHED
CHILD
Table 16 A
Sl. Signs of well nourished Signs of ill nourished child
No child
1. Skin is smooth, pliable and Lack of colour of skin –
elastic and of a healthy paleness
colour.
2. Bright and clear eyes and Pale, dark red, or purple
pink eye membranes. mucous membrane lining the
eyes. Failing eye sight.
3. Firm pink nails Rigid brittle nails.
4. The hair is lustrous and Dull hair lacking sheen, dry,
firmly attached to the and can be easily plucked.
scalp.
5. Healthy gums and Pale, dark red or purple
membranes of the mouth. colour of gums.
6. Reddish pink tongue. Not Sores on skin, lip or tongue,
coated, pink lips. pale lips.
7. Desirable height for age Stunted growth and weight
and desirable weight for deficit.
height.
8. Good appetite and sound Loss of appetite, digestive
nutrition. disturbances, undernutrition.
9. Normal body temperature, Above normal body
pulse rate and breathing temperature, shortness of
rate. breath while performing
normal activity.
10. Healthy children are alert. Listless, irritable and
depressed.

135
 Fig 16.1 An ill nourished Indian child
Source : Human Nutrition: Principles and Applications In
India, by Mc Divitt.M.E. and Mudambi S.R., 1973

136
 Fig 16.2 A well – nourished Indian child
Source : Human Nutrition: Principles and Applications In
India, by McDivitt .M.E.and Mudambi S.R., 1973
137
16.5 NEED FOR AND METHODS OF ASSESSING
NUTRITIONAL STATUS
Nutritional status is the condition of health of the individual
as influenced by the utilization of the nutrients. It can be
determined by correlation of information obtained through
medical and dietary history, thorough physical examination and
laboratory investigation.
Nutritional assessment aids in identifying
a) Under Nutrition
b) Over Nutrition
c) Nutritional deficiencies
d) Individuals at the risk of developing malnutrition
e) Individuals at the risk of developing nutritional
related diseases
f) The resources available to assist them to
overcome nutritional problems.
The nutritional status can be assessed by the following
methods:
I Direct Methods
a) Nutritional Anthropometry
b) Clinical Examination
c) Biochemical tests and
d) Biophysical methods.
II Indirect Methods
a) Vital statistics of the community
b) Assessment of socio – economic status and
c) Diet surveys

16.6 ANTHROPOMETRIC MEASUREMENTS AND

INDICES
Nutritional Anthropometry is concerned with the
measurements of the variations of physical dimensions and body
composition at stages of life cycle and different planes of
nutrition. It is a field-oriented method, which can be easily
adopted and interpreted.
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 The basic measurements which should be made on all
age groups are weight in kg, length / height and arm
circumference in cms. In young children it should be
supplemented by measurements of head and chest
circumference.
Weight:
Weight gain is an indicator of growth in children. It is
measured with the help of the weighing scale. Body weight
should be determined after the first void and before ingestion of
food.
The weight for age can be compared with the standards
of ICMR and the nutritional status can be interpreted.
The standard reference body weight (kg) of Indians of
different age groups is given in the table 16 B
Table 16 B
Reference body weight (kg) of Indians of different Age
groups
Reference body weight (kg)
Age (years) Male Female
Infants 0–½ 5.4 5.4
Children ½– 1 8.6 8.6
1–3 12.61 11.81
4–6 19.20 18.69
7–9 27.00 26.75
10 – 12 35.54 37.91
Adolescents 13 –15 47.88 46.66
16 – 18 57.28 49.92
Adults 20 – 50 60 50
Source : ICMR 2002. Nutrient Requirements and recommended
dietary allowances for Indians. NIN.
Anthropometric Indices : Weight for age The Nutritional
status can be interpreted using Gomez Classification as follows
Weight > 90% Weight for age. Normal.
76 – 90% Weight for age. Grade I malnutrition.
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 61 < 75% Weight for age. Grade II malnutrition.
< 60% Weight for age. Grade III malnutrition.
Linear Measurements
Two types of linear measurements are commonly used.
(i) height or length of the whole body
(ii) circumference of the head and the chest.
Height :
The height of the individual is the sum of four
components: leg, pelvis, spine and skull.
Table 16 C
The Standard Reference height for Indians of different age
groups.
Age (years) Height in cm
Boys Girls
1+ 80.07 78.09
2+ 90.01 87.93
3+ 98.36 96.21
4+ 104.70 104.19
5+ 113.51 112.24
6+ 118.90 117.73
7+ 123.32 122.65
8+ 127.86 127.22
9+ 133.63 133.08
10+ 138.45 138.90
11+ 143.35 145.00
12+ 148.91 150.98
13+ 154.94 153.44
14+ 161.70 155.04
15+ 165.33 155.98
16+ 168.40 156.00
Source : ICMR 2002. Nutrient Requirements and recommended
dietary allowances for Indians. NIN.
The height of an individual is measured using a
stadiometer.
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 For infants and children recumbent length (crown – heel
length) is measured. The measurement is compared with the
standards of the ICMR as given in table 16C to assess nutritional
status.
The desirable birth weight and length of an infant is 3 kg
and 50 cm respectively. By the time the baby turns the first birth
day, the birth weight is doubled and an increment of 25 cm in
length is reached.
Changes in body weight from birth till one year

Fig 16.3
Head Circumference :
The measurement of head circumference is a standard
procedure to detect pathological condition in children. Head
circumference is related mainly to brain size. At birth the
circumference of head is greater than that of the chest.

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 .

Fig 16. 4 Measuring head circumference

Source: Jelliffe, D.B., 1989, The Assessment of Nutritional
Status of the community WHO Monograph Series, Geneva
Chest Circumference :
The circumference of the head and the chest are about
the same at six months of age. After this the skull grows slowly
and the chest more rapidly.
Therefore between the ages of six months and five years
the chest / head circumference ratio of less than one may be due
to failure to develop or due to wasting of muscle and fat of
chest.
In nutritional anthropomertry the chest / head
circumference ratio is of value in detecting under nutrition in
early childhood.
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 Fig.16.5 Measuring chest circumference
Source: Jelliffe, D.B., 1989, The Assessment of Nutritional
Status of the community WHO Monograph Series, Geneva
Mid Upper Arm Circumference (MUAC) :
Mid upper arm circumference at birth in a healthy child
is between 10 – 11cm. over the first year the increment in
MUAC is 3 to 4 cm as the muscles of the arms start to develop.
In the preschool age the increase in MUAC is only one cm.
Hence there is not much difference between the MUAC of a 3
year old from that of a 5 year old. So MUAC is an age
independent index. The field workers in nutrition in our country
have fixed the desirable value for MUAC as 12 cm for Indian
preschool children.
The WHO has recommended 14 centimeter as a
desirable value for MUAC for preschool children.
Hence in screening malnourished children in a
community this method is used with ease. When the value of

143
MUAC is less than 12 cm among 1 –5 year old children, they
are designated as malnourished.
In the field condition a bangle with a diameter of 4
centimeter can be used as a tool to detect malnutrition. When the
bangle moves smoothly over the mid-upper arm of the child, it
indicates malnutrition. The bangle test can be conducted with
ease in field condition to screen malnourished children.

Fig. 16.6 Measuring mid upper arm circumference

Source: Jelliffe, D.B., 1989, The Assessment of Nutritional
Status of the community WHO Monograph Series, Geneva

16.7 CLINICAL SIGNS OF NUTRITIONAL DEFICIENCY

DISORDERS
Clinical examination is an important practical method for
assessing the nutritional status of a community. Essentially, the
method is based on examination for changes, believed to be
related to inadequate nutrition that can be seen or felt in the
superficial epithelial tissues especially the skin, eyes, hair and
buccal mucosa or in organs near the surface of the body such as
the parotid and thyroid glands.
144
 Clinical assessment must always be carried out by
individuals with adequate training. The following simple guide
is employed to interpret the following deficiencies.

Guide for the interpretation of deficiencies and identifying

the clinical signs.

Condition Clinical Signs

(i) Protein Energy : Odema, depigmentation,

Malnutrition sparseness and easy
pluckability of hair, moon
face, enlarged liver, muscle
wasting.

(ii) Vitamin A : Night blindness, Bitot’ s spots

deficiency in the eye, Xerosis of skin.

(iii) Riboflavin : Angular stomatitis, cheilosis.

deficiency
(iv) Thiamine : Oedema, sensory loss, calf
deficiency muscle tenderness.
(v) Niacin deficiency : Raw tongue, pigmentation of
the skin.
(vi) Vitamin C : Spongy and bleeding gum.
deficiency
(vii) Vitamin D : Rickets, beading of ribs,
deficiency Knock – knees, bowed legs.

(viii) Iron deficiency : Pale conjunctiva, spoon –

shaped nails.
(ix) Iodine deficiency : Enlargement of thyroid gland.
Source: Jelliffe, D.B., 1989, The Assessment of Nutritional
Status of the community WHO Monograph Series, Geneva

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Biophysical Methods:
The biophysical methods are used to assess the
alterations in functions associated with inadequate nutrition. For
(eg) Dark adaptation test is used to evaluate the ability to see in
the dim light.
Biochemical test:
Biochemical tests can be used to detect the deficiencies
by analyzing blood, urine, stools and phlem. For (eg)
Estimation of hemoglobin in blood to detect iron deficiency.

Indirect Methods:
Vital Statistics:
Malnutrition influences morbidity, mortality, life
expectancy and other health statistics. Hence vital statistics may
therefore be considered as indirect indication of the nutritional
status of the community.
Infant mortality rate, maternal mortality rate and
morbidity rate are the vital statistics that can be used to assess
the nutritional status of the community.
Assessment of socio – economic status: -
Low food availability, increased family size, unsanitary
living conditions, inadequate knowledge of nutritional needs,
inappropriate weaning practices are powerful social cultural and
economic factors, which influence nutritional status.
Diet surveys:
Diet surveys are helpful in studying the quality and
quantity of food consumed by the family and the community.
The techniques of collecting information on family food
consumption include:
1) Food Inventory Method: This method is usually
employed in Institutions where homogenous group of
people take their meals in a common kitchen eg.
Hostels, orphanages. In this method the amount of
food stuff issued to the kitchen as per the issue register
146
 is taken into consideration. No direct measurement or
weighing is done. A study period of one week is
desirable.
2) Food expenditure pattern method
In this method information on the amount spent on
food and non-food items during the previous month or
week is collected using a questionnaire. This method
avoids actual weighing of foods.
3) 24 hour recall
In this method a set of standardized cups suited to local
conditions are used. The standard cups help the
respondent to recall the quantities of the food prepared
and fed to individual members on the previous day.
This is usually done for three consecutive days. The
advantage of this method is that the intake of each food
item by the specific individual in the family such as
pre-school child, adolescent, pregnant women can be
assessed using the cups.
4) Diet History:
This method is useful for obtaining qualitative details
of diet and studying patterns of food consumption at
household and industrial level. The procedure includes
assessment of the frequency of consumption, different
foods, daily or number of times in a week or fortnight
or occasionally. This method is used to study meal
pattern, dietary habits, food preferences, and
avoidances during sickness.
5) Weighment method:
In this method, the food either raw or cooked is
actually weighed using an accurate balance. It is ideal
to conduct the survey for seven consecutive days.
Every day food is weighed in the morning and evening
before actual cooking. The age, sex, physiological
status of the family members should be noted down.
Nutrient intake is then calculated using the ICMR food
147
 composition tables. Though this method is accurate as
the foods are directly weighed, it requires extreme co-
operation of the house wives.
The information on food and nutrient consumption is
compared with the recommended allowances of the ICMR and
the adequacy is determined. A combination of dietary, clinical
and biochemical assessment is desirable for assessment of
nutrition status of individuals or communities.

Questions

Part-A
Fill in the blanks:
1. Good nutrition is required to maintain ______________
2. ___________ is impairment of health resulting from
deficiency, excess or imbalance of nutrients.
3. Diet survey is an ______________ of assessing the
nutritional status of the community.

Part-B
Write short answers:
1. Define nutrition, nutritional status and health.
2. Explain what is mal nutrition?
3. How will you differentiate between a well-nourished and
ill nourished child?

Part-C
Write detailed answers:
How will you assess the nutritional status of your class
using nutritional anthropometry methods?

148
 17. CARBOHYDRATES
Energy that is needed to move, perform work and live is
chiefly consumed in the form of carbohydrates. Carbohydrates,
primarily starches, are least expensive, easily obtained and
readily digested form of fuel.
17.1 COMPOSITION
Carbohydrates are organic compounds composed of
carbon, hydrogen and oxygen, with the later elements in the
ratio of 2:1. The general formula is CnH2nOn. They are viewed as
hydrated carbon atoms.
17.2 CLASSIFICATION – SIMPLE AND COMPLEX :

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 Carbohydrates are classified, depending on the number
of sugar units they contain, as simple carbohydrate and complex
carbohydrates.
Monosaccharides and disaccharides make up simple
carbohydrates, called simple sugars containing one and two
sugar units respectively. Polysaccharides called complex
carbohydrates are structurally larger and more complex than
simple sugars. They include starch, dietary fibre and glycogen.
There are two main classes of monosaccharides based on
the carbonyl group present in them. They are aldoses and
ketoses, aldoses (eg; glucose) containing the aldehyde group
(CHO) and ketoses, (eg;- fructose) containing the ketone group
(C=O).
Aldoses are further divided into trioses, tetroses,
pentoses and hexoses based on the number of carbon atoms.
The common disaccharides are Maltose, Lactose and
Sucrose which on hydrolysis yield two monosaccharide units.

Maltose hydrolysis Glucose + Glucose

Lactose hydrolysis Glucose + Galactose

Sucrose hydrolysis Glucose + Fructose

Polysaccharides have high molecular weight and

are insoluble in water. They are in the form of long chains either
branched or unbranched.
The polysaccharides are further classified into
groups depending upon the products they yield on hydrolysis.
Homopolysaccharides yield only one type of monosaccharide
units on hydrolysis eg:- starch, dextrin, cellulose, glycogen.
Heteropolysaccharides yield more than one type
of monosaccharide units on hydrolysis eg:- Heparin, Hyaluronic
acid. Heparin is an anticoagulant found in the liver, spleen,
lungs and blood. Hyaluronic acid is found in the umbilical cord,
150
synovial fluid and vitreous humour. It has a lubricating action.
In tissues it forms an important part of the cementing ground
substance.
The sugars are also classified as reducing and non
reducing sugar. The reducing property is attributed to the free
aldehyde or keto group.

17.3 FUNCTIONS AND FOOD SOURCES

Carbohydrates perform the following functions.
1. Energy :
The principle function of carbohydrates is to serve as a
major source of energy for the body. Each gram of carbohydrate
yields 4Kcal of energy regardless of its source. In Indian diets
60 – 80 % of energy is derived from carbohydrate.

2. Glucose:
Glucose is indispensable for the maintenance of the
functional integrity of the nervous tissue and is the sole source
of energy for the proper functioning of the brain.
Prolonged lack of glucose may cause irreversible damage
to the brain.

3. Protein Sparing Action :

Carbohydrates exert a protein sparing action. If sufficient
amounts of carbohydrates are not available in the diet, the body
will convert protein to glucose in order to supply energy.
Hence to spare proteins for tissue building, carbohydrates
must be supplied in optimum amounts in the diet. This is called
the protein sparing action of carbohydrates.
4. Fat Metabolism :
Carbohydrates are essential to maintain normal fat
metabolism.
Insufficient carbohydrates in the diet results in larger
amounts of fat being used for energy than the body is equipped
151
to handle. This leads to accumulation of acidic intermediate
products called ketone bodies.

5. Synthesis of Body Substances

Carbohydrates aid in the synthesis of nonessential
aminoacids, glycoproteins (which function as antibodies) and
glycolipids (which form a part of cell membrane in body tissues
especially brain and nervous system).
Lactose remains in the intestine longer than other
disaccharides and thus encourages growth of beneficial bacteria.

6. Precursors of Nucleic Acid :

Carbohydrates and products derived from them, serve as
precursors of compounds like nucleic acids, connective tissue
matrix and galactosides of nervous tissue.

7. Detoxification Function :
Glucuronic acid, a metabolite of glucose serves as a
detoxifying agent.
It combines with harmful substances containing alcohol or
phenolic group converting them to harmless compounds which
are later excreted.

8. Roughage of the Diet :

Insoluble fibres known as composite carbohydrates can
absorb water and give bulk to the intestinal contents which aids
in the elimination of waste products by stimulating peristaltic
movements of the gastrointestinal tract.

Food Sources of Carbohydrates

Cereal grains, roots and tubers are the major sources of
starch. Fruits and vegetables contain varying amounts of
monosaccharides and dissaccharides.
Sugar is obtained from sugarcane.
Types and sources of Carbohydrates are given in the table-17A.
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 Table 17A
Types and Sources of Carbohydrates
Carbohydrate Food Source
1. Monosaccharides
Glucose Fruits, honey, corn-syrup.
Fructose Fruits, honey.
Galactose, Maltose These do not occur in free
form in foods.
2. Dissaccharides
Sucrose Cane and beet sugar.
Lactose Milk and milk products.
Maltose Malt and Cereal products.
3. Polysaccharides
Digestible:
Starch & Dextin Grains, vegetables especially
roots& tubers and legumes
Glycogen Meat products and seafoods
Indigestible: Stalks and leaves of
Cellulose vegetables, outer coat of seeds
Pectins, Gums Fruits, Plant secretions and
seeds.

17.4 DIGESTION, ABSORPTION AND UTILIZATION

Digestion :
The first stage of digestion of carbohydrate takes place in
the mouth. Chewing breaks up food and exposes starch and
sugars to the action of enzymes.
Saliva contains salivary amylase (ptyalin). It converts
starch to maltose but time limits the action of salivary amylase,
because as food enters the stomach, the acid present in the
stomach blocks the action of salivary amylase.
In the stomach the acid causes hydrolysis of sucrose. In the
small intestine pancreatic amylase and intestinal amylase digest
starch up to the stage of maltose.
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Starch salivary, pancreatic maltose + Isomaltose
& intestinal amylase

Glycogen is also broken by these enzymes to

dissacharides. Enzymes maltase, sucrase and lactase present in
the brush borders of the columnar cells of small intestine
convert dissacharides to monosaccharides.

Maltose Maltase Glucose + Glucose

Lactose Lactase Glucose + Galactose

Sucrose Sucrase Glucose + Fructose

Cellulose and other polysaccharides are not digested by

enzymes, so undigested material passes to large intestine
forming bulk which contributes to faeces.
The end products of carbohydrate digestion are
monosaccharides – glucose, galactose and fructose.
They are absorbed by process of active absorption by the
mucosa of the small intestine.

Metabolism:
Metabolism occurs inside the various cells of the body.
There are two types of metabolism; anabolism (building up) and
catabolism (breaking down).
The major carbohydrate anabolic path ways are conversion
of glucose into glycogen (glycogenesis) in the liver and muscles.
The conversion of glucose into fat (lipogenesis) in the liver and
adipose tissue.
Carbohydrates follow two major catabolic pathways:
The breakdown of glucose releasing energy (glycolysis)
and converting it into usable energy (ATP) and the
conversion of glycogen to glucose (glycogenolysis).
154
 After digestion and absorption of glucose into the blood
stream it is utilized directly by the tissues for energy.
When the absorbed glucose exceeds the body’ s need for
energy it is stored as glycogen in the liver and muscle and
excess glucose is converted to triglycerides and stored as fat in
adipose tissue.

17.5 REQUIREMENTS
As carbohydrate is utilized as main source of energy, at
least 40 percent of the total energy in the food should come from
Carbohydrates.
In our country 60 – 80 percent of a day’ s energy needs are
met from carbohydrates in the form of starch furnished by
cereals and pulses.
In developed countries only 30 – 40 percent of days energy
needs are met from carbohydrates.
17.6 DIETARY FIBRE
Dietary fibre is defined as that portion of plant material
ingested in the diet that is resistant to digestion by gastro
intestinal secretions. It consists of hemicellulose, cellulose,
lignins, oligosaccharides, pectins, gums and waxes.
Some bacteria in the large intestine can degrade some
components of fibre releasing products, that can be absorbed
into the body and used as energy source.
Two categories of fibre are found in food. Crude fibre is
defined as the residue remaining after the treatment with hot
sulphuric acid, alkali and alcohol.
The major component of crude fibre is a polysaccharide
called cellulose. Crude fibre is a component of dietary fibre.
Several other carbohydrate and related compounds called
pectins, hemicellulose and lignins are the second category found
in plant foods and are also resistant to digestion.
These together with cellulose are collectively known as
dietary fibre.
155
Types of fibre:
There are two types of fibre – soluble and insoluble fibres.
The food source and action of these in the body is given in the
table-17B.
Table 17 B

Types and Sources of fibre.

Types of Major food sources Action in the

fibre body
Soluble • Delay gastro
fibres Citrus fruits, apple, intestinal
Gums, oats, barley, legumes transit
pectins, • Delay glucose
mucilages absorption
• Lower blood
cholesterol

Insoluble Whole wheat products, •Accelerate

fibres wheat bran, whole Gastro intestinal
Cellulose grain breads, cereals transit
hemicellulose and vegetables like • Increase fecal
green peas, beans weight
cabbage. • Slow starch
Lignin hydrolysis
Skin of vegetable and • Delay glucose
fruits, grains absorption

Questions

Part A
Fill in the blanks:
1) Formation of glycogen from non carbohydrate sources is
called ____________
156
 2) Extra calories from glucose are deposited in the body as
___________
3) The starch splitting enzyme is __________
4) Saliva contains the enzyme _________ which acts up on
starch
5) The hormone __________ secreted by the cells of islets
of langerhans helps in the utilization of sugar by the
tissues.

Part B
Write short answers:
1) What are amylases ?
2) State three good sources of cellulose.
3) Give examples of two disaccharides and their source in
diet.
4) List the functions of carbohydrates.

Part C
Write detailed answers:
1) Describe the digestion of carbohydrates.
2) Classify Carbohydrates. Write in detail about the main
properties of various classes of Carbohydrates.

157
 18. PROTEIN
One fifth of an adults total body weight is protein.
Protein is found in every cell of our body.
All the tissues in our body such as muscle, blood, bone,
skin and hair are made up of proteins.
Many hormones and enzymes are either protein or
protein derivatives. The nucleic acids in the cell nucleus occur in
combination with proteins as nucleoproteins.
Protein is thus essential to maintain cellular integrity and
function and for health and reproduction.
18.1 COMPOSITION
Proteins contain carbon, hydrogen, oxygen and nitrogen.
They are distinguished from carbohydrates and fats by the
presence of nitrogen.
Protein is synthesized from basic units called amino
acids. Protein molecules, which contain up to hundred amino
acids are much larger than carbohydrates or lipid molecule.
Chemically amino acids are composed of a carbon atom
to which is attached a carboxyl (COOH) group, a hydrogen atom
(H), an amino group (NH2) and an amino acid radical (R) as
shown below.
Structure of an Amino acid.
COOH
H C R
NH2
The carboxyl group, the amino group and the hydrogen
atom are the same for all amino acids.
The R group however distinguishes one amino acid from
another.
R varies from a single hydrogen atom as found in
glycine, to longer chain of up to 7 carbon atoms.
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 A protein molecule is made upon of chains of amino
acids joined to each other by a peptide linkage. The amino group
of one amino acid is linked to the carboxyl group of another
amino acid by removal of water.
Thus two amino acids form di-peptide and three form a
tri-peptide. Proteins consist of hundreds of such linkages hence
called Polypeptides.

18.2 ESSENTIAL AND NON-ESSENTIAL AMINO ACIDS

Amino acids are classified into two groups – essential
(indispensable) and non-essential (dispensable).
An essential amino acid is one that cannot be synthesized
by the body to meet the physiological needs and hence should be
supplied by the diet. The essential amino acids are histidine,
isoleucine, leucine, lysine, methionine, phenylalanine threonine,
tryptophan and valine. Non-essential amino acids are those that
the body can synthesize. They are alanine, arginine, aspargine,
aspartic acid, cysteine, glutamic acid, glutamine, glycine,
proline, serine and tyrosine.

18.3 BIOLOGICAL VALUE OF PROTEIN

Biological value of protein is the percentage of a protein
nitrogen that is absorbed and available for use by the body for
growth and maintenance.
Proteins are functionally divided into complete, partially
complete and incomplete proteins. A complete protein contains
all essential amino acids in relatively the same amounts as
human beings require to promote and maintain normal growth.
(eg) Protein derived from animal foods. A partially complete
protein contains sufficient amounts of amino acids to maintain
life but fail to promote growth. (eg) Gliadin in wheat.
Incomplete proteins are incapable of replacing or building new

159
tissue and cannot support life or growth. (eg) Protein in Wheat
germ.
The quality of a protein is determined by the kind and
proportion of amino acid it contains. Proteins that contain all
essential amino acids in proportions capable of promoting
growth are described as complete protein, good quality protein,
or proteins of high biological value.
A good quality protein is digested and utilized well. Egg
protein is a complete protein and is considered as a reference
protein with the highest biological value. The quality of other
proteins is determined based on their comparison with egg
protein as in figure 18.1

The eight essential amino Wheat germ is an incomplete

acids (EAA) must be present protein because it is
in a protein in specific ratios. deficient in tryptophan
Egg protein has all eight in the (incomplete circle). As a
result of this deficiency only
correct proportions used most
less of the total protein can
efficiently and completely by be used (as represented by
the body. the inner dotted circle).

Fig.18.1 Comparison of complete protein with incomplete

protein
160
 The protein of animal foods like milk, meat, and fish
generally compare well with egg in the essential amino acid
composition and are categorized as good quality proteins.
Plant proteins are of poor quality, since the essential
amino acid composition is not well balanced.
The amino acid, which is not present in sufficient
amount in food protein, is called the limiting amino acid of that
food. For (eg) Lysine in cereal protein, Tryptophan in Wheat
germ.
Table 18 A
Biological value of food proteins.
Food Stuff Biological Value
I Animal Protein
Egg 96
Milk 90
Meat 74
Fish 80
II Vegetable Protein
Cereals
Rice 80
Wheat 66
Maize 50
Pulses
III
Bengal gram 74
Red gram 72

IV Oil Seeds
Ground nut 55
Gingelly 62

Source: Gopalan.C, B.V.Rama Sastri and Balasubramaniam.

S.C.1996 (reprinted) Nutritive Value of Indian foods, NIN.
Hyderabad.
161
 The limitation in cereals can be overcome by a judicious
combination with pulses, which are rich in lysine.
The resulting mixture of cereals and pulse will have an
amino acid pattern better than either of the constituents. Thus a
combination of cereal and pulse has a supplementary effect. For
(eg) recipes like, Pongal, Idli, dhokla are based on cereal pulse
combination.
Thus the habitual diets in India based on cereal and pulse
have indeed a rational basis. The biological value of some
important food proteins is given in table-18A.
18.4 FOOD SOURCES
All foods except refined sugar, oils and fats contain
protein to varying degree.

Table 18 B
Food Sources of Dietary Protein
Food Stuff Protein %
Rich Sources :
Meat, fish and liver 18 – 20
Eggs 14
Milk powder, full fat 26
Milk powder, skimmed 33
Cheese 18 – 20
Pulses 18 – 24
Nuts and oilseeds 18 – 26
Soyabean 35 – 40
Good Sources :
Cereals and millets 6 – 12
Tender legumes, green peas, cow peas 7 – 8
Fair Sources :
Potato 2
Green leafy vegetables 2–6
Source : Swaminathan. M. 1986. Principles of Nutrition and
Dietetics.
162
 Animal foods like meat, fish, egg and plant foods like
pulses oilseeds and nuts contain high amounts of proteins and
are classified as rich sources of proteins.
Cereals and millets and tender legumes such as green
peas are moderate sources of protein.
However cereals are consumed in large amounts daily
and contribute a considerable amount of protein to the daily
intake.
Leafy vegetables, roots and tubers are poor sources of
protein as they contain less than two percent proteins.
The protein content of foods are listed in the table-18B.
18.5 FUNCTIONS OF PROTEIN
Proteins form a major part of total body structures and
they participate in many activities in our body. The major
functions of protein in our body can be listed as in the
table-18C.
Table 18 C
Functions of Protein
1. Build and repair Proteins form integral parts of most
body tissues body structure such as skin, tendon,
membranes, muscles, organs and
bones. They support the growth and
repair of body tissues.
2. Enzymes (eg) Lipase helps to breakdown fat
and sucrase breaks down sugar.
3. Hormones Regulate body process.
4. Antibodies Inactivate foreign invaders thus
protecting the body against disease.
5. Fluid & Proteins help to maintain the volume
electrolyte and composition of body fluids.
balance
6. Acid-base Proteins help maintain the acid-base
balance balance of the body fluids by acting as
buffers.
163
 7. Energy Proteins provide fuel for the body’ s
energy needs [4 KCal/gm].
8. Storage Proteins help to store iron and copper.
9. Homeostasis Proteins maintain normal osmotic
balance among body fluids.
10. Transport These type of proteins carry nutrients
proteins (eg) to the tissues. eg lipoprotein carry
Haemoglobin, lipids, haemoglobin transports oxygen.
lipoprotein
11. Contribute to Proteins impart colour , flavour, odour
sensory & and texture to foods.
physical
properties of
food

18.6 DIGESTION, ABSORPTION AND UTILIZATION OF

PROTEINS :
Proteins taken in the diet are digested to amino acids in
the stomach and small intestine. Gastric juice contains enzymes
pepsin which digests protein in acid medium. It hydrolyses
proteins to polypeptides.
Dietary Protein Pepsin Polypeptides

In the small intestine, pancreatic and intestinal juices

contain proteolytic enzymes. Pancreatic juice contains trypsin,
chymotrypsin and carboxyl peptidase.
They hydrolyse large protein molecule to smaller
polypeptide.
Proteins Trypsin and Peptides + Amino acids
Chymotrypsin
Peptides Carboxy peptidase Aminoacids
Amino Peptidase

164
 Intestinal juices contains polypeptidases & dipeptidases
which hydrolyse polypeptide & dipeptide to individual amino
acids. There are several peptidases acting on different proteins.
When undigested protein enter large intestines, bacteria causes
nitrification of proteins leading to foul smelling flatus.

Dipeptides Dipeptidase Amino acids

Tripeptides Tripeptidase Amino acids

Proteins are mainly absorbed in the form of amino acids.

Amino acids are absorbed by active transport mechanism in the
intestinal cells. Sometimes whole protein may be absorbed by
the mechanism of pinocytosis.

Absorbed amino acids pass into the portal blood and

reach liver where they are converted to proteins. Other amino
acids are transported through general circulation and are utilized
for protein synthesis in the tissues.

Utilization of proteins in the body :

The amino acids from digested proteins are absorbed
rapidly into the blood and passed onto different tissues to meet
their needs.
Some non-essential amino acids are synthesized in the
liver and also released into the circulation. The amino acids
released by hydrolysis of tissue proteins are also added to the
amino acid pool in the body.
The protein metabolism of mammals is in a dynamic
state and the synthesis and breakdown of tissue protein takes
place constantly.
The unwanted amino acids are oxidized in the liver to
yield energy and urea. The dynamic aspects of protein
metabolism are represented as follows.

165
 Dynamic aspects of Protein Metabolism

18.7 REQUIREMENTS
The Indian Council of Medical Research
recommends 1.0 g/kg/day as the safe level of intake in terms of
dietary protein for Indians.
During pregnancy and lactation additional allowances
are recommended. Protein requirements for children vary
depending on body weight and expected weight gain.
The ICMR recommended dietary allowances for
proteins is given in table-18D.

166
 Table 18 D
ICMR Recommended Dietary Allowances for Proteins
Group Protein g/day
Man 60
Woman 50
Pregnant woman 50 + 15
LACTATION
0-6 months 50 + 25
6-12 months 50 + 18
INFANCY
0-6 months 2.05 /kg
6-12 months 1.65 /kg
CHILDREN
1-3 Yrs 22
4-6 Yrs 30
7-9 Yrs 41
BOYS
10-12 Yrs 54
13-15 Yrs 70
16-18 Yrs 78
GIRLS
10-12 Yrs 57
13-15 Yrs 65
16-18 Yrs 63
18.8 EFFECTS OF DEFICIENCY
Deficiency of energy and protein commonly occur in
developing countries like India. This is manifested as Marasmus
and Kwashiorkor. Protein Energy Malnutrition (PEM) is a term
used to describe clinical disorders resulting from varying
degrees of protein and energy deficiency.
Kwashiorkor is due to quantitative and qualitative
deficiency of protein in diet in which energy intake is adequate.
Marasmus is due to continued restriction of energy intake.
167
 PEM is prevalent in all parts of the World and in all
ages. It is primarily a disease that occurs in young children who
live in poverty. In India PEM is the most widespread form of
malnutrition among pre-school children. A majority of them
suffer from varying grades of malnutrition.
As many as 43.8 percent of pre-school children suffer
from moderate degrees of PEM and 8.7 percent suffer from
extreme forms of malnutrition. The paths leading from early
weaning to Nutritional marasmus and from protracted breast
feeding to kwashiorkor is depicted below.

Breast feeding

Early Abrupt weaning Late gradual weaning

Dilute dirty formula Starchy family diet

Repeated Infection Acute Infection

Especially gastro enteritis

Starvation therapy

Nutritional Marasmus Marasmic Kwashiorkor

Kwashiorkor

Source : Passmore. R. and Eastwood. M.A1990. Human

Nutrition and Dietetics.

Clinical symptoms of Protein Energy Malnutrition

1) Failure to grow accompanied by thinning, weakening
and wasting of muscles.
2) Behavioural changes ranging from the irritability of
kwashiorkor to the apathy of marasmus.

168
 3) Oedema which is the accumulation of fluid in the tissues
making them soft and spongy.
4) Skin changes including changes in colour, lack of colour,
peeling and ulceration.
5) Changes in hair which becomes dry and sparse and takes
on a characteristic red color (Flags syndrome).
6) Loss of appetite, Vomitting, diarrhoea resulting in
dehydration.
7) Enlargement of the liver.
8) Anaemia
9) Increased susceptibility to infection and fever.

Fig 18.2 Marasmic child

Source : Venkatachalam.P.S and Rebello.R.M. 1996 Nutrition
for Mother and Child. NIN; ICMR.
169
 Fig 18.3 A child with Kwashiorkor
Source : Venkatachalam.P.S and Rebello.R.M. 1996 Nutrition
for Mother and Child. NIN; ICMR.
170
 Fig 18.4 A child after 12 weeks of treatment
Source : Venkatachalam.P.S and Rebello.R.M. 1996 Nutrition
for Mother and Child. NIN; ICMR.
171
 Questions

Part A
Fill in the blanks:
1. Proteins are distinguished from Carbohydrate and fat by
the presence of __________.
2. Amino acid that cannot be synthesized in the body is
called ________.
3. Histidine is a __________ amino acid.
4. __________ protein is the reference protein.
5. Amino acids are linked together by _________linkages.
6. Deficiency of Protein is manifested as ________.

Part B
Write short answers:
1. What are polypeptides ?
2. What are essential and non-essential aminoacids?
3. Explain Limiting aminoacids with an example.
4. How will you determine the protein quality of food?
5. What is reference protein?

Part C
Write detailed answers:
1. Discuss the causes of Kwashiorkor and Marasmus?
2. Discuss proteins under the following heads
a. Digestion b. Absorption c. Utilization
3. List the functions of protein.

172
 19. LIPID

Lipids more commonly known as fats and oil, are

integral part of our food. They are insoluble in water but soluble
in organic solvents. They occur in both plant and animals.
Lipids are a concentrated source of energy.

19.1 CLASSIFICATION
Lipids are classified into simple, compound and derived
lipids which are further subdivided as follows.

Lipids

Simple Compound Derived

a) Fats and oils (a) Phospho lipids (a)Sterols
b) Waxes (b) Glyco lipids (b)Fatty acids
(c) Lipo proteins
Simple lipids
Fats and Oils are included in this type. At room temperature, oils
are liquids and fats are solids. Fats and oils contain esters of
fatty acid and glycerol, a form in which lipids are present in
food.
Compound lipids
They are esters of fatty acids containing phosphorous
carbohydrate or protein. Phospholipids contain a phosphoric
acid in addition to the alcohol and fatty acids.
Glycolipids contain a fatty acid, carbohydrate and a
nitrogenous base. Phospholipids and glycolipids form part of the
cell membrane and the nervous system.
Lipoproteins are macromolecular complex of lipids with
proteins.
173
Derived lipids
These are substances liberated during hydrolysis of
simple and compound lipids which still retain the properties of
lipids. The important members of this group are sterols, fatty
acids and alcohol.
Sterols
Sterols are solid alcohols and form esters with fatty
acids. In nature they occur in the free state in the form of esters.
Based on their origin sterols are classified as cholesterol (animal
origin) and phytosterol (in plants).
Cholesterol is a complex type of lipid that is regularly
synthesised by and stored in the liver. It is present in all animal
products.
Fatty acids
Fatty acids are the main building blocks of fat. They
have a methyl group (CH3) at one end and a carboxyl group
(COOH) at the other end with a chain of carbon and hydrogen
atom in the middle. They have a basic formula CH3(CH2)n
COOH. Where ‘ n’ denotes the number of carbon atoms which
may vary from 2 to 2l.
Fatty acids can be classified into Saturated Fatty
Acids(SFA) & Unsaturated Fatty Acids (UFA)
Saturated Fatty Acids
Saturated fatty acids are those that are unable to absorb
more hydrogen. They are usually stiff and hard fats. Eg. Ghee,
Butter.
Unsaturated fatty acids
Unsaturated fatty acids have one or more double bond in
their molecule and are thus not saturated with hydrogen. They
are liquid at room temperature. Eg. Sunflower oil.
Unsaturated fatty acids may be monounsaturated or
polyunsaturated depending on the number of double bonds.
Monounsaturated fatty acids (MUFA)
MUFA have only one double bond in their molecule. Eg.
oleic acid found in olive oil, peanut oil.
174
Polyunsaturated fatty acids(PUFA)
PUFA have 2 or more double bonds in their molecule.
Eg. linoleic acid, linolenic acid. They are present in corn,
safflower, soyabean, sunflower oils and fishoils.
Monounsaturated and polyunsaturated fats are usually
soft or liquid at room temperature.
Triglycerides
Fatty acids combine with glycerol to form a glyceride,
When only one fatty acid combines with glycerol, it forms a
monoglyceride, diglycerides have 2 fatty acids and triglycerides
have three fatty acids attached to glycerol.
Most of the fatty acids present in the body and absorbed
from foods occur in the form of triglycerides.
During digestion triglycerides are hydrolysed to form
free fatty acid, monoglycerides and glycerol which are absorbed
by the intestinal wall and the majority of these are rebuilt as
triglycerides.
Long and short chain fatty acid
The number of carbon atom in fatty acids decides the
chain length. Thus short chain fatty acids contain 4 to 6 carbon
atoms, medium chain 8 to 12 carbon atoms and long chain fatty
acid have 14 to 18 carbon atom.
Essential and Non – Essential fatty acid
Essential fatty acid (EFA) are those which cannot be
synthesized by the body and need to be supplied through diet.
Linolenic acid, linoleic acid and arachidonic acid are
essential fatty acids.
Non -essential fatty acids
Non-essential fatty acids are those which can be
synthesized by the body and which need not be supplied through
the diet. Palmitic acid, oleic acid and butyric acid are examples
of non – essential fatty acids.
19.2 COMPOSITION
Fat is a complex molecule constituting a mixture of fatty
acids and an alcohol, generally glycerol.
175
 It contains carbon, hydrogen and oxygen but differs from
carbohydrates in that it contains more carbon and hydrogen and
less oxygen.
When one gram of fat is oxidized it yields 9 kilocalories

19.3 FUNCTIONS OF LIPIDS

Lipids perform several important functions:
1) They are the concentrated fuel reserve of the body
2) Lipids are the constituents of cell membrane structure
and regulate the membrane permeability.
3) They are essential for the digestion, absorption and
utilization of fat soluble vitamins like Vitamin A, D, E
and K.
4) Lipids are important as cellular metabolic regulators
(Steroid hormones and prostaglandin).
5) Lipids protect the internal organs serving as insulating
materials.
6) As compounds of the mitochondria membranes, lipids
(phospholipids) participate in electron transport chain.
7) Fat imparts palatability to the diet and slows stomach
emptying time, thus giving a feeling of fullness. This
delay of onset of hunger is called ‘ satiety value’ of fats.
8) The calories in fat spare the proteins from being oxidized
for energy.
9) Fat deposited in the adipose tissue serve as reserve
source of energy during starvation. It acts as an insulator
conserving the body heat.
Essential fatty acids which are derived lipids, perform
important functions in our body.

Functions of essential fatty acids:

1) Maintenance of the function and integrity of cellular and
subcellular membrane.
2) Regulation of cholesterol metabolism by transporting it
between the blood and body tissues.
176
 3) Acts as precursor of hormone like – prostaglandin which
aid in regulating vascular function and help relieving
pain and inflammation.
4) Delays blood clotting time.

19.4 DIGESTION ABSORPTION AND UTILIZATION

Digestion
In the mouth:
Fat digestion starts in the mouth with hard fats
beginning to melt when they reach body temperature.
The salivary glands at the base of the tongue release a
lipase enzyme which digest fat to a less extent in adults.
In the stomach:
In the stomach fat floats as a layer above the
others components of swallowed food. As a result little
fat digestion takes place.
In the small Intestine:
When fat enters the small intestine, the hormone
cholescystokinin signals the gall bladder to release bile.
Bile emulsifies fat and also provides an alkaline medium
for the action of pancreatic lipase and intestinal lipase.
The triglycerides are acted upon by these lipases and
hydrolyzed to monolycerides and fatty acids.
The cholesterol esters are hydrolyzed to give cholesterol
and fatty acids.

Triglycerides Monoglyceride + fatty acids

Cholesterol esters Cholesterol + fatty acids

Absorption and Utilisation

Small molecules of digested triglycerides (glycerol, short
& medium chain fatty acids) can diffuse into intestinal cells and
are absorbed directly into the blood stream.
Larger molecules(Monoglycerides, long chain fatty acids) merge
into spherical complexes known as miscelles. The lipid contents
177
of the miscelles diffuse into the intestinal cells. Once inside the
monoglycerides and long chain fatty acids are reassembled to
new triglycerides.
Within the intestinal cells the new triglycerides and
larger lipids like cholesterol and phospholipids are placed into
transport vehicle called chylomicrons.
The intestinal cells then release chylomicrons into the
lymphatic system. The lymph circulation empties into the
thoracic duct which inturn enter the subclavian vein and
subsequently into the blood stream.
The blood transport lipids to the rest of the body and
cells absorb them and utilize for energy. This breakdown of fat
to yield energy is called lipolysis.
Majority of lipids enter via the lymph to the liver where
the protein and lipid (cholesterol, triglycerides) are bound
together to form lipoproteins.

There are four types of lipoproteins, they are:

i) chylomicrons,
ii) very low density lipo protein (VLDL)
iii) low density lipo protein (LDL) and
iv) high density lipo proteins (HDL).
Chylomicrons, VLDL and LDL serve to transport and
deposit lipids from the intestine and liver to the tissues for
absorption. Low-density lipoprotein, which has the highest
cholesterol fraction favours lipid deposition in tissues including
blood vessels and hence termed ‘ bad’ cholesterol. HDL
cholesterol removes the lipids from the tissues and transports it
back to liver for disposal, hence it is termed as ‘ good
cholesterol’ . High levels of LDL cholesterol indicates a high
risk of cardiovascular disease.
Apart from lipoproteins, triglycerides, cholesterol and
phospholipids are synthesized in the liver. This is called
lipogenesis.
It can be represented as follows:
178
 Absorbed fat

Lymph Systemic
circulation (chylomicrons) circulation (glycerol short
and medium chain fatty
acids

Liver

Adipose Muscle
Synthesis Modification Tissue
triglycerides of Short chain
phospho fatty acids Oxidation
lipids. (lipolysis)
Lipo proteins Synthesis or
cholestrol hydrolysis Energy
Oxidation +CO2+H2O

Acetoacetate
Source : Robinson C..H., Marilyn. R. and Lawler 1982.
Normal and therapeutic nutrition.

19.5 FOOD SOURCES

Foods in general contain two types of fat namely “visible
fats” and “invisible” or “hidden” fats.

Visible fats
Visible fats are fats extracted from the following sources.
a. Oil seeds : coconut, corn, cornseed, groundnut, mustard,
palm, rice bran, safflower, seasame,
soyabean, sunflower and hydrogenated
vegetable oils (vanaspathi).
b. Animal fats: Butter and Ghee.
c. Fish oils: Shark and cod liver oils.
179
Invisible or hidden fats:
Invisible or hidden fats are those which form an integral
part of foods and are therefore not visible. It includes the fats
present in the cells and cell walls and cell membranes of both
plant and animal tissues.
Almost everything we eat as listed below carries some
invisible fats.
a) Plant food – Cereals, millets, vegetables, spices, nuts and
oil seeds, coconut, avacado.
b) Animal food – Milk and milk products (curd, cream,
cheese), flesh foods, (mutton, beef, pork, chicken) organ
meats (brain, liver, kidney), fish, shrimp, prawn.
Sources of Saturated Fat:
Saturated fat is resistant to oxidation even at frying
temperatures. Examples are
a) Plants – coconut oil. Hydrogenated vegetable oils. Palm
kernel oil.
b) Animals – Butter, ghee, fats from flesh foods and organ
meats.
Sources of Unsaturated Fat:
Unsaturated fats and oils include mono unsaturated fatty
acids and PUFA in various proportions.
Important sources of unsaturated fats are as follows:
Plant sources:
All common vegetable oils with the exception of coconut
oils are predominantly unsaturated. The invisible fats present in
nuts and oilseeds, cereals, pulses and legumes, roots and tubers,
vegetables, spices and fruits.
In most plant foods and vegetable oils linoleic acid is the
predominant PUFA, but mustard and soyabean oils,
legumes/pulses. Fenugreek leaves, and green leafy vegetables
are good sources of alpha linolenic acid.
Animal sources:
The muscles (lean meat) of flesh foods, unlike the depot
fat surrounding the tissues is mainly composed of cholesterol
180
esters and phospholipids, both of which have a high proportion
of long chain n-6 PUFA which are otherwise formed in the body
from linolenic acid.
Arachidonic acid is found in animal and human cells.
Fish and fish oils provide long chain n – 3 PUFA.
Hydrogenation:
Hydrogenation (addition of hydrogen at double bonds)
converts liquid oils into semisolid or solid fats. During
hydrogenation, linoleic and alpha linolenic acid present in the
oils are converted to trans fatty acids and saturated fatty acids.
Also, the monounsaturated fatty acids are converted to saturated
fatty acids.
Hydrogenated fats were designed to imitate ghee. It is
used to prepare processed foods like biscuits and cakes.
Vanaspathi is produced in India by hydrogenation of vegetables
oils.
Cholesterol:
Cholesterol is a constituent of animal foods but is absent
in plants. Vegetable oils do not have cholesterol. In human diets,
cholesterol is obtained from ghee, butter, cheese, milk, curd,
egg, flesh foods, organ meats, fish and prawns. Most animal
foods are good sources of both cholesterol and fatty acids.
Requirements
The ICMR recommended allowances for fat for Indians is given
in table 19A.
Table 19 A
The ICMR recommended allowances for fat.
Group Fat g/day
Adult man 20
Adult woman 20
Pregnant woman 30
Lactating woman 45
Children (1–9 yrs) 25
Children (10–18 yrs) 22

181
Effects of Deficiency:
Deficiency of fat in the diet causes the deficiency of
essential fatty acids. Deficiency of essential fatty acids leads to
cessation of growth.
It also results in flaky skin, development of itchy sores
on the scalp. The common disorder in adults and children in
India is phrynoderma or toad skin.
The condition is characterized by the presence of horny
eruptions on the posterior and lateral aspects of the limbs on the
back and buttocks.
Phrynoderma is cured rapidly by the administration of
linseed or safflower seed oil rich in EFA.
Infants fed on a EFA deficient diet develop irritation and
changes in the skin with in a few weeks. The skin changes
appear as dryness and desquamation with oozing in the folds.
Diarrhoea may also occur, supplementation of the diet
with linoleic acid helps to restore the skin to normal condition.

Questions

Part A
Fill in the blanks:
1. Fats are digested in ___________
2. The physiological fuel value of fat is ____________
3. Macromolecular complexes of lipids with proteins are called
____________
4. _________ is also called the good cholesterol.
5. Fats which form an integral part of food is called _________
fat.
6. One gram of fat when oxidized yields _________ Kcal.
7. Cholesterol and phospholipids are transported via vehicles
called ________

182
 Part B
Write short answers:
1. Write short notes on visible and invisible fat.
2. What are essential and non – essential fatty acids.
3. Classify lipids and discuss their properties.
4. Enumerate the functions of essential fatty acids.
5. Write short notes on phrynoderma.

Part C
Write detailed answers:
1. Enumerate the functions of lipids.
2. How is lipid digested, absorbed and utilized in our body.
3. Discuss the various food sources of lipids.

183
 20. ENERGY
Energy is the capacity to do work. The energy to perform
work is derived from the carbohydrate, fat and protein in the
diet. The source of energy in diets varies depending on
agricultural, cultural, social and economic factors.
The body needs energy for maintaining body
temperature, metabolic activity, supporting growth, for physical
work, to maintain constant body weight and good health.
The body’ s storage energy or potential energy is
continuously available in the body from the glycogen in muscle
and liver. This stored energy is transformed to other forms to
accomplish the work of the body. Examples are
I. Osmotic Energy – Maintain transport of nutrients.
II. Electrical Energy – Transmission of nerve impulse.
III. Chemical Energy – Synthesis of new compounds.
IV. Thermal Energy – Heat regulation.
Whenever one form of energy is produced another form
is reduced by exactly the same amount as stated by the Law of
Conservation of Energy.
This law states that energy can neither be created or
destroyed it can only be transformed from one form to another.

20.1 UNITS OF ENERGY – CALORIE AND JOULE

The unit of energy, kilocalorie (Kcal) was used for a
long time. Recently the International Union of Sciences and
International Union of Nutritional Science (IUNS) have adopted
‘ Joule’ as the unit of energy in place of Kcal. These units are
defined as follows.
A joule is defined as the energy required to move 1kg
mass by 1 metre by a force of 1 Newton acting on it.
One Newton is the force needed to accelerate 1 kg mass
by less than a second.
184
 Kcal is defined as the heat required to raise the
temperature of 1kg of water by 1° C. (From 14.5°C to 15.5°C)
1Kcal = 4.184 KJ (Kilo Joules)
1000 Kcal = 4184 = 4.18 MJ ( mega joules )
1 KJ = 0.239 Kcal.

20.2 ENERGY VALUE OF FOODS

The energy in various foods is measured by calorimetry.
Calorimetry is the measurement of heat loss.
An instrument for measuring heat output of the body or
the energy value of foods is called a Calorimeter. In measuring
the calorie value of foods, Bomb calorimeter is used. The
maximum amount of energy that the sample is capable of
yielding when it is completely burnt or oxidized is the energy
value of that food, also known as heat of combustion.
The energy measured using a Bomb Calorimeter is as
follows
1g of Carbohydrate - 4.1 kcal
1g of fat - 9.45 kcal
1g of protein - 5.65 kcal

When samples of Carbohydrate, Fat, Protein are

burned, the amount of heat produced is always the same for each
of these nutrients.
In the bomb calorimeter carbohydrates, fats and
proteins are completely oxidized whereas in the human body the
process of digestion and absorption does not proceed with 100
percent efficiency. The extent of digestion varies from one
nutrient to another.
The Coefficient of digestibility is used to express the
proportion of an ingested nutrient that ultimately becomes
available to the body cells.
The coefficient of digestibility for carbohydrate, fat and
protein are 0.98, 0.95 and 0.92 respectively. It is observed that
carbohydrate and fat are metabolized almost completely,
185
Whereas protein metabolism is incomplete due to the presence
of nitrogen. The physiological energy value of carbohydrate, fat
and protein are 4, 9 and 4.
These values are known as Atwater Bryant factors or
physiological fuel values as given in table-20A.

Table 20 A
Physiological fuel value of Carbohydrate, Fat, Protein
Heat of Coefficient Digestibility Physiolog
Combustion of percent ical fuel
Kcal digestibility value
Kcal
Carboh 4.1 0.98 98 4.0
ydrate
Fat 9.45 0.95 95 9.0
Protein 5.65 0.92 92 4.0
Source : Robinson C. H., Marilyn R. and Lawler. 1982.
Normal and Therapeutic Nutrition.

20.3 BASAL METABOLISM

Basal Metabolism is the minimum amount of energy needed
by the body for maintenance of life when the person is at post
absorptive state, physical and emotional rest.
Basal Metabolic Rate (BMR) is a measure of the energy
required by the activities of resting tissue.
The Basal Metabolic rate can be measured directly from the
heat produced (using a Respiration Calorimeter and Metabolic
Chamber) or indirectly from O2 intake and CO2 expenditure
when the subject is at rest.

Factors affecting Basal Metabolic Rate (BMR)

The factors affecting Basal Metabolic Rate are listed
in table-20B.

186
 Table 20 B
Factors affecting Basal Metabolic Rate (BMR)

Factor Effect on BMR

Body Compositon The more lean body mass higher is the
BMR. This is due to greater metabolic
activity in these tissues when compared to
bones and fat. Men with a high
proportion of muscle mass or lean body
mass have a higher BMR than women.
Fever Fever raises the BMR. There is a 7%
increase in BMR for each degree rise in
temperature in Fahrenheit.
Stress Stress raises BMR.
Smoking & Increases the BMR
Caffeine
Hyperthyroidism The basal metabolic rate is elevated as
(Oversecretion of much as 50-70%.
thyroxin)
Growth In children and pregnant women the BMR
is higher.
Pregnancy During the last trimester of pregnancy
Basal Metabolic rate is increased by 15-
25% as there is a increase in muscle mass
of uterus, size of mammary gland, foetal
mass and placenta, cardiac work and
respiratory rate.
Fasting/Starvation Lowers BMR
Hypothyroidism The basal metabolic rate is decreased by
(under secretion of 30%
thyroxin)
Age Lean body mass diminishes with age
slowing the BMR. In tall people the
BMR is higher.
Undernutrition Prolonged undernutrition lowers the
BMR.
187
20.4 ENERGY COST OF PHYSICAL ACTIVITIES
Next to Basal Metabolism it is the physical activity, which
accounts for the largest energy expenditure.
There is a wide variation from individual to individual in
occupational activity.
The energy required for the actual physical activity varies
depending on the type of occupation of an individual.
For computing energy requirements, the occupations have
been classified as sedentary, moderate and heavy.
The ICMR classification of activities based on occupation is
given in table-20C.
Table 20 C
Classification of activities based on occupation
Sex Sedentary Moderate Heavy
(80-180 Kcal / hr ) (170-240 Kcal / hr ) (250-350
Kcal / hr )
1) Teacher 1) Fisherman, 1) Stone
Male 2) Tailor 2) Potter Cutter,
3) Executive 2) Mine
Worker.
3) Wood
Cutter.
1) Teacher 1) Servant maid. 1) Stone
Female 2) Executive 2) Weaver Cutter
3) Nurse
Source : ICMR 2002 Nutrient requirements and recommended
dietary allowances for Indians, NIN
The energy cost of physical activities is expressed in terms of
BMR units.
BMR Unit :
The energy cost of rest and physical activity is expressed
as multiples of BMR which is called the physical activity ratio
(PAR).
The physical activity ratio expresses the energy cost of
an individual activity per minute as ratio of the cost of BMR per
188
minute. Hence it is advantageous to express the energy
expenditure in terms of BMR units.

Table 20 D
Energy cost of some common activities in terms of BMR
Units.
Activity Energy cost of activities in
BMR Units
Sitting quietly 1.2
Standing quietly 1.4
Sitting at desk 1.3
Walking (3MPH) 3.7
Source : ICMR 2002 Nutrient requirements and recommended
dietary allowances for Indians, NIN
Using factorial method the WHO / FAO expert committee has
derived the BMR factors for Indian men and women as 1.6, 1.9
& 2.5 respectively for the three categories of activities namely,
sedentary, moderate & heavy as given in table-20D.
Table 20 E
Energy requirements of Indian Adults in terms of BMR
Units.
Activity Duration Rate of energy expenditure in
(hrs) terms of BMR Units
Sedentary Moderate Heavy
Sleep 8 1.0 1.0 1.0
Occupational 8 1.7 2.8 4.5
activity
Non- 8 2.2 2 -
Occupational
Activity
Average for 1.6 1.9 2.5
24 hr
Source : ICMR 2002 Nutrient requirements and recommended
dietary allowances for Indians, NIN
189
20.5 THERMIC EFFECT OF FOOD
Food ingestion stimulates metabolism and requires energy
to meet the multiple activities of digestion, absorption and
transport of nutrients.
This overall stimulating effect of food is called dietary
thermogenesis or thermic effect of food (formerly called as
specific dynamic action (SDA). The increase in energy cost
because of thermogenesis is 10%.

20.6 ESTIMATION OF TOTAL ENERGY NEEDS

The energy requirement of an individual is the level of
energy intake from food that will balance energy expenditure
when the individual has a body size and composition and level
of physical activity, consistent with long term good health, and
that will allow for maintenance of economically necessary and
socially desirable activity. In children and pregnant and
lactating women, the energy requirement includes the energy
needs associated with the deposition of tissues or the secretion
of milk at rates consistent with good health. (WHO)
Energy requirements are best determined by measurements of
energy expenditure.
Energy expenditure from a physiological point of view is
made up of three major components:
(i) BMR (ii) Dietary Thermogenesis (iii) Physical
activity
For all practical purposes, the component of energy
expenditure related to regulatory energy output or dietary
thermogenesis are known to merge into measurements related to
the cost of physical activity. Hence energy expenditure has only
two principal component i) BMR & ii) Physical activity.
Calculation of energy requirements.
1) Predicting BMR.
Equations for predicting BMR (K.cal / 24 hrs) as
proposed by the ICMR expert committee for Indians is given
in table-20E.
190
 Table 20 F
Equations for predicting BMR (Kcal / 24 hr)
Sex Age Prediction Equation
(yrs)
18 – 30 14.5 x B.W (kg) + 645
Male 30 – 60 10.9 x B.W (kg) + 833
> 60 12.8 x B.W (kg) + 463
18 – 30 14.0 x B.W (kg) + 471
Female 30 – 60 8.3 x B.W (kg) + 788
> 60 10.0 x B.W (kg) + 565
Source : ICMR 2002 Nutrient requirements and recommended
dietary allowances for Indians, NIN
2) Calculating Daily energy requirement:
Using the computed BMR from body weights and
recommended BMR factor for Indians for different levels of
physical activity (which is 1.6, 1.9 & 2.5 for sedentary,
moderate & heavy activity respectively) the energy requirements
are arrived at.

For example:
For an Indian adult man 29 yrs of age, weighing 60kg
and doing moderate activity, the energy requirement is
calculated as follows.

1) BMR = 14.5 x 60 + 645 = 1515 k.cal / 24 hr

2) Energy requirement = predicted BMR x BMR units
for activity = 1515 x 1.9
= 2878.5
2878 k.cal / day.

20.7 ENERGY BALANCE

To maintain daily energy balance the total energy
requirement of an individual is the number of K cal necessary to
replace daily basal metabolic loss in addition to loss from
exercise and other physical activities.
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 Fig 20.1 Energy Balance

Source : Susan. R. Holman, 1987. Essential of Nutrition for the

Health Professionals.
Obesity represents an energy imbalance resulting from
an excess of energy input over energy output (expenditure).
Extreme weight loss is a state of energy imbalance resulting
from a deficit energy input over energy output.

Questions

Part A
Fill in the blanks:
1. The unit of energy is ____________.
2. The energy value of foods is measured using a _______.
3. 1 gm of carbohydrate yields ________ kcal.
4. 1 gm of fat yields ___ kcal.
5. Protein is not digested completely due to _________
6. Fever _________BMR.
7. A teacher is classified as doing ________activity.
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 8. The rate of energy expenditure in terms of BMR units
for moderate activity is _____.

Part B
Write short answers:
1. Define Kcal.
2. What is Basal Metabolic rate?
3. What is dietary thermogenesis?
4. Define BMR unit.
5. Give the physiological fuel value of carbohydrate,
protein & fat.

Part C
Write detailed answers:
1. Discuss the factors influencing basal metabolic rate.
2. How are daily energy requirements arrived at?

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 21. MINERALS AND VITAMINS
Until the middle of the nineteenth century, the
importance of minerals and vitamins was not known. It was
observed that carbohydrate, fat, protein alone were incapable of
promoting and sustaining growth.
Hence scientists attempted to find out the “missing
elements”, namely minerals and vitamins which are essential for
growth and maintenance.
Essential minerals which are inorganic substances are
classified as macro and micronutrients based on the amount
needed by humans per day.
Macrominerals are those which are vital to health and
that are required in the diet by more than 100mg per day and
those required in the diet less than 20mg per day are called
microminerals or trace minerals.
The essential microminerals are Calcium, Phosphorous,
Magnesium, Sulphur, Potassium and Chloride. Important
microminerals of relevance in human nutrition are Iron, Zinc,
Copper, Sodium, Cobalt, Fluoride, Manganese, Chromium,
Iodine and Molybdenum.

21.1 FUNCTIONS, FOODSOURCES, REQUIREMENTS

AND EFFECTS OF DEFICIENCY.

Calcium and Phosphorus:

Calcium is an essential element required for several life
processes. The requirements of Calcium and Phosphorous are
considered together as their function and requirement are closely
linked.
Over 99% of the Calcium and Phosphorous is present in
the bones and the remaining 1% in the body fluids.
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 The Calcium and Phosphorous are present in the ratio of
2:1 in our body. In the skeletal system Ca and P is present in the
form of hydroxyapatite crystals.
Hydroxyapatite is a compound made up of calcium and
phosphate that is deposited into the bone matrix to give it
strength and rigidity.
Functions :
1. Bone formation:
The major mineral ions of the bone are Calcium,
Phosphorous and Magnesium. For proper calcification of
bones, (deposition of minerals on the bone matrix) which
occurs during the growing years, adequate supply of
these minerals is essential.
2. Tooth formation: - Calcium and Phosphorous together as
a compound is essential for the formation of dentin and
enamel.
3. Physiological Process: -
a. Calcium is essential for the clotting of blood as it
is required for prothrombin activation.
b. Calcium regulates the permeability of the
capillary walls and ion transport across the cell
membranes.
c. It is essential for the contraction of the heart and
skeletal muscle.
d. Ca regulates the excitability of the nerve fibres.
e. Ca acts as an activator for enzymes such as
rennin and pancreatic lipase.
4. Phosphorous is essential for the storage and release of
adenosine triphosphate (ATP) molecules.
5. Phosphates plays an important role as buffers to prevent
changes in acidity of the body fluids.
6. Phoshpolipids are major components of cell membrane
and intra cellular organelles.
7. In the DNA and RNA phosphate is an essential part of
the nucleic acids.
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Food sources
Among cereals ragi contains large amounts of calcium.
Bengalgram whole, gingely seeds, cuminseeds, poppy seeds,
agathi, amaranth, drumstick leaves are good sources of calcium.
Milk and milk products are good sources of calcium and
phosphorous. Only 20 – 30 % of the calcium in the diet is
absorbed, which is facilitated by Vitamin – D. All foods contain
significant amounts of phosphorous.
Requirements
The recommended dietary allowances for Calcium, as
suggested by the ICMR is given in table-21A.
Table 21 A
ICMR recommended dietary allowances for Calcium
Age Group RDA for calcium mg/day
Infant 0 – 12 months 500
Children 1 – 9 years 400
Children 10 – 15 years 600
Adolesent 16 – 18 years 500
Adult 400
Pregnant women 1000
Lactating women 1000

Deficiency
Calcium related health problems occur due to inadequate
intake, improper absorption or utilization of calcium.
Osteoporosis:
Osteoporosis is a condition found primarily among
middle aged and elderly woman, where the bone mass of the
skeleton is diminished.

It is a condition of multiple origin. It results due to the

following reasons:
(i) Prolonged dietary inadequacy
(ii) Poor absorption and utilization of calcium

196
 (iii) Immobility
(iv) Decreased levels of oestrogen in post menopausal
women.
(v) Hyper parathyroidism
(vi) Vitamin – D deficiency

Osteomalacia – is a condition in which the quality but not the

quantity of bone is reduced. This condition is dicussed in detail
under deficiency of Vitamin – D.

Tetany
Tetany occurs when Calcium in the blood drops below
the critical level. There is a change in the stimulation of nerve
cells resulting in increased excitability of the nerve and
uncontrolled contraction of the muscle tissue. Hence Calcium
and Phosphorous ratio in the diet should be maintained at 1:1 for
proper utilization of Calcium in the body.

Microminerals
Microminerals are also known as trace elements. The
microminerals are Iron, Iodine, Zinc, Copper, Fluoride,
Selenium, Chromium, Manganese, cobalt and Molybdenum.
However only the deficiency of few of these elements is
observed in humans. Iron and Iodine deficiencies are wide
spread while deficiency of Cu, Zn, Cr and Se have been
reported in recent years.

Iron
The total body iron is 4g in adults. Iron exists in a
complex form in our body. It is present as
a) Iron porphyrin compounds – hemoglobin in RBC,
myoglobin in muscle.
b) Enzymes – (eg) peroxidases, succinase dehydrogenase
and cytochrome oxidase.

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 c) Transport and storage forms: - (eg) transferrin and
ferritin.
Functions :
The chief functions of iron in the body are :
1. Iron forms a part of the protein – haemoglobin which
carries oxygen to different parts of the body.
2. It forms a part of the myoglobin in muscles which makes
oxygen available for muscle contraction.
3. Iron is necessary for the utilization of energy as part of
the cells metabolic machinery.
4. As part of enzymes iron catalizes many important
reactions in the body. Examples are
a) Conversion of beta carotene to active form of
Vitamin A
b) Synthesis of carnitine, purines, collagen and neuro
transmiters.
c) Detoxification of drugs in the liver.

Food Sources
The iron present in food can be as haem and non-haem
iron depending upon the source from which it is obtained. Haem
iron – is obtained from animal tissues, non-heam iron – is
obtained from plant foods.
Sources of non-haem iron are ragi, green leafy
vegetables, dried fruits and jaggery. Liver, fish, poultry, meat,
eggs dates are good sources of haem iron .
Haem iron is absorbed and utilized better than the non-
haem iron. Iron absorption from Indian diets is only 3 percent as
it is mainly cereal based diet.

Requirement
Iron requirements for various age groups is listed in table-21B.
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 Table 21 B
ICMR – Recommended dietary Allowances for Iron
Group Iron requirement (mg/day)
Birth – 1 year 1
1 – 5 year 15 – 20
6 – 12 years 15 – 20
13 – 18 years
Boys 25
Girls 35
Man 20
Woman 30
Pregnancy 40
Lactation 30
Deficiency
Dietary iron deficiency leads to nutritional anaemia.
Nutritional anaemia is defined as the condition that results from
the inability of the erythropoetic tissue to maintain a normal
haemoglobin concentration.
Anaemia occurs when the haemoglobin level falls below
12 gm /dl in adult man and woman. During pregnancy
haemoglobin level below 11 gm /dl is termed anaemia.
Nutritional anaemia is the common form of anaemia
affecting women in reproductive years, infants and children
which is mainly due to poor intake and absorption.
Iron deficiency anaemia is wide spread in our country.
The prevalence varying from 45% in men and 70% in women
and children. The major cause of anemia in India is because of
Iron and folic acid deficiency.
Nutritional anemia is manifested as :
1. Reduced Haemoglobin level. (less than 12 g /dl)
2. Defects in the structure, function of the epithelial tissues
3. Paleness of skin and the inside of the lower eyelid is pale
pink

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 4. Finger nails becoming thin and flat and eventually
(spoon shaped nails) koilonychia develops.
5. Progressive untreated anaemia results in cardiovascular
and respiratory changes leading to cardiac failure. The
general symptoms include lassitude, fatigue,
breathlessness on exertion, palpitations, dizziness,
sleeplessness, dimmness of vision, and increased
susceptibility to infection.

Iodine
Iodine is an essential constituent of the thyroid hormone
produced by the thyroid glands. It occurs as free iodide ions or
as protein bound iodine in our body. About 15 – 23 mg of iodine
is present in the adult human body.
The body store of iodine is predominantly present in
thyroid gland and also in salivary gland, mammary glands
gastric glands and in kidneys to a certain extent.

Function
Iodine is essential for the synthesis of the thyroid
hormones T3 and T4.

Sources
Richest source of iodine are sea foods like sea fishes and
common salt from sea water. Iodine content of vegetables, fruits
and cereals depends upon the iodine content of the soil in which
they grow. The soil of mountaineous regions contains less
iodine.

Requirement
The ICMR recommended dietary allowance for Iodine is
150 µg/day.
Deficiency
Iodine deficiency in the diet, causes enlargement of the
thyroid gland called as “goitre”. Goitre occurs in people staying
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in hilly regions where the iodine content of water and soil is
comparatively less.
In India goitre is common in hilly districts of Himalaya.
Goitre can be treated by administration of iodine. If treatment is
given in early stages goitre can be corrected.
Severe iodine deficiency in children leads to
hypothyroidism resulting in retarded physical and mental
growth. This condition is known as cretinism.
Goitrogens are substances present in foods which cause
goitre. These substances react with iodine present in the food
making it unavailable for absorption. Foods like cabbage,
cauliflower, raddish contain goitrogens.
Zinc
Zinc is primarily intracellular substance. Its total
quantity in the body is 2.3g. Largest stores of Zinc is present in
the bones. Zinc forms a constituent of the blood. Zinc is an
important element performing a range of function in the body as
it is a cofactor for a number of enzymes.
Functions
1. Zinc is a constituent of enzymes such as carbonic
anhydrase, alkaline phosphatase, lactic dehydrogenase.
2. It is a constitutent of the hormone insulin
3. It plays a major role in the synthesis of DNA and
proteins.
Sources
Meat, unmilled cereals and legumes are good sources.
Fruits and vegetables are poor sources.
Requirements
The daily requirement of Zinc in adults is 15.5 mg / day
as recommended by the ICMR expert group.
Apart from iron, iodine, zinc, copper, selenium and
fluorine are essential trace elements. Copper is essential element
in iron absorption.
Selenium is an essential element along with Vitamin E
for maintaining integrity of the liver cells. Fluorine is required in
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minimum amounts to prevent dental caries. Excessive
consumption leads to mottling of teeth.

Vitamins
Vitamins are organic substances present in small
amounts in food, they are required for carrying out vital
functions of the body. They are involved in the utilization of the
major nutrients like proteins, fats and carbohydrates.
Though needed in small amounts, they are essential for
health and well being of the body. When these Vitamins were
discovered on the basis of their function and before their
chemical nature were elucidated, they were designated as
A,B,C,D or in terms of their major functions like, antineuritic,
antirichitic Vitamins. Vitamins are classified based on their
solubility as fat soluble and water soluble vitamins.

21.2 CLASSIFICATION OF VITAMINS

Vitamins

1. Fat soluble 2. Water soluble

Vitamin A,D,E,K Vitamin B1,B2,B6,B12
(Niacin) Nicotinic Acid
FolicAcid and Vitamin- C.
Water soluble vitamins are not accumulated in the body , but are
readily excreted while fat soluble vitamins are stored in the
body. For this reason excessive intake of fat soluble vitamins,
especially Vitamin A and D can prove toxic. Excessive intake
leads to the condition called hypervitaminosis.
21.2.1 Functions, food sources, requirements and effects of
deficiency
Fat soluble vitamins:
Vitamin A
Vitamin A was the first fat soluble vitamin to be
recognized. Three forms of Vitamin A are active in the body,
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retinol, retinal and retinoic acid. They are collectively called as
retinoids .
Beta carotene is the provitamin of Vitamin A.
Provitamins are substances that are chemically related to a
vitamin but must be changed by the body into the active form of
the vitamin. Vitamin A in the diet comes in two forms.
Retenoids (preformed Vitamin A) and carotenoids.
Vitamin A is present in vegetable foods which contain yellow
pigment called carotenes. It was isolated from carrots hence
called carotenoids which are provitamins of Vitamin A.
Functions.
1) A well understood function of retinol is in the
visual process. The retina of the human eye
contains two distinct photo receptors of which
one is sensitive to light intensities . Vitamin A is
essential for the formation of rhodopsin and
normal functioning of the retina for clear vision
in dim light. Lack of Vitamin A leads to impaired
adaptation to darkness.
2) Participates in protein synthesis and cell
differentiation and thereby maintaining the health
of the epithelial tissues and skin.
3) Supports reproduction and growth
4) Vitamin A regulates the antibodies and cellular
immune response. It is essential for maintaining
the epithelial tissue which is the first line of
defence against invading microorganism.
5) Beta carotene acts as an antioxidant capable of
protecting the body against disease like cancer,
cardiovascular diseases and cataract.

Sources
Vitamin A in the human diet exist as retinol or as retinal
or beta carotene which has to be converted to Vitamin A. Foods
of animal origin contain retinol.
203
 Plant sources are rich in Beta carotene. Only one third of
the dietary beta Carotene is absorbed.
Beta Carotene from green leafy vegetables is well
utilized than from carrots and papayas.
Good sources of Vitamin A are sheep liver, butter, ghee,
egg, milk, curds, liver oils of shark and halibut.
Good sources of beta carotene are agathi, amaranth,
drumstick leaves, green leafy vegetables, mango, papaya, carrot
and jack fruit.

Requirements:
The ICMR recommended dietary allowance for retinol is given
in table-21C.
Table 21 C
ICMR recommended dietary allowance for Retinol
Group Retinol µg/day

Man 600
Woman 600
Pregnant 600
women
Lactation 950
Infants 350
Children 400-600
Effects of Deficiency
Deficiency of Vitamin A is manifested as nutritional
blindness and increased susceptibility to infection. Nutritional
blindness is an important public health problem among young
children in India .
Night blindness is an early symptom of Vitamin A
deficiency. The individual cannot see in dim light. This can be
corrected with adequate supply of Vitamin A. In the absence of
adequate Vitamin A intake the outer lining of the eye ball loses
its usual moist, white appearance and becomes dry and wrinkled
called xerosis.
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 This condition is followed by raised muddy dry
triangular patches on the conjunctiva called the bitots spots.
Redness and inflammation of the eye and gradual loss of vision
may follow. The central portion of the eye loses its transparency
and becomes opaque and soft if not treated and leads to total
blindness termed Xeropthalmia. Xeropthalmia encompasses all
ocular manifestations of Vitamin A deficiency.
Increased susceptibility to infection occurs because the
mucous membrane lining becomes dry and rough which is easily
invaded by the micro – organism.
Hypervitaminosis
Intake of large amount of Vitamin A for prolonged
periods can lead to toxic symptoms which include irritability,
headache, nausea and vomitting.
Vitamin D
Vitamin D can be synthesized in the body in adequate
amounts by simple exposure to sunlight, even for 5 minutes per
day is sufficient.
It is essential for bone growth and calcium metabolism.
It acts as a hormone in the body by facilitating calcium
absorption and deposition in the bone.
Functions
1. Vitamin D helps in the absorption of calcium and
phosphorous by increasing the synthesis of calcium
binding protein.
2. Vitamin D helps to maintain the calcium and
phosphorous levels in the body by stimulating,
a) Absorption in the gastro intestinal tract.
b) Retention by the kidney
3. Vitamin D helps in deposition of calcium in the bones.
The bones grow denser and stronger.
Food Sources
The Vitamin D content of food sources from animals
varies with the diet, breed and exposure to sunlight of the
animal.
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 The good sources of Vitamin D are cod liver oil, shrimp,
liver, butter, yolk, cheese, milk, spinach and cabbage.
Requirements
The expert group of ICMR has not recommended dietary
intake of Vitamin D for Indians.
Only in those cases where the Vitamin D requirement is
not met due to inadequate exposure to sunlight the ICMR
recommends 400 µg/day of Vitamin D .
Deficiency
Deficiency of Vitamin D leads to decreased absorption
of calcium which is manifested as muscular tetany, rickets in
children and osteomalacia in adults.
Due to faulty calcification of bones the following
derfomities is manifested in children which is called rickets. It
is a disease in which there is weakness and abnormalities in
bone formation. Rickets primarily affects children.

Fig 21.2 Vitamin D deficient child-Rickets

Source : Venkatachalam.P.S and Rebello.R.M. 1996 Nutrition
for Mother and Child. NIN; ICMR.
206
Manifestations:
a) faulty deposition of calcium on the bones.
b) Bowing of legs
c) Enlargement of ends of long bones
d) Deformities of ribs – beading of ribs
e) Delayed closing of frontanel
f) Slow erruption of teeth.
g) Malformed, decay – prone teeth
Osteomalacia in Adults
Osteomalacia is a condition where the quality of the
bone is reduced. It occurs in women who are not exposed to
sunshine and who have depleted mineral reserves resulting from
successive pregnancies and prolonged lactation.
Osteomalacia is associated with low phosphorous level
but low blood calcium level is the most frequent cause.
The following symptoms occur
1. softening of the bones
2. deformities of the limbs, spine, thorax and pelvis
3. demineralization of the bones
4. pain in pelvis, lower back and legs
5. frequent bone fractures.
Hypervitaminosis
As in the case of Vitamin A intake of excessive amounts
of Vitamin D leads to toxic symptoms which include irritability,
nausea , vomiting and constipation.
Vitamin E
Vitamin E is known as antisterility vitamin because it is
required for normal reproduction in animals and men.
Functions
1. Vitamin E is the primary antioxidant in the body and
serves to protect polyunsaturated fatty acids (PUFA)
from oxidation in cells and maintain integrity of the cell
membrane. It also prevents the oxidation of beta carotene
and Vitamin A. Vitamin E helps to maintain cell
membrane integrity and protect RBC against hemolysis.
207
 2. Vitamin E reduces platelet aggregation
3. Vitamin E is essential for the iron metabolism and the
maintenance of nervous tissues and immune function.
4. Vitamin E is been promoted as an anti-aging vitamin,
because as cells age they accumulate lipid breakdown
products. Vitamin E prevents this accumulation in
maintaining cell health.
Food Sources
Vitamin E is widely distributed in foods. It is present in
high concentration in vegetable oils and in cereal grains. Wheat
gum, sunflower seeds, almonds, safflower oil, eggs, butter are
good sources.
Meat, fruits and vegetables contain small amounts.
Sesame oil and mustard oil are good sources of Vitamin E.
Requirement :
The requirement of Vitamin E is linked to that of
essential fatty acids (linoleic and linolenic acids). The
requirement of Vitamin E is 0.8 mg/g of essential fatty acid.

Deficiency
1. Prolonged intake of Vitamin E deficient diets produces
uncoordinated movement, weakness and sensory
disturbances.
2. It causes haemolytic anaemia in low birth weight infants
3. Defective functioning of the retina leading to permanent
blindness in premature infants occurs.
4. It leads to reproductive failure in humans
5. Vitamin E deficiency is associated with decreased ability
of the lymphocytes.
Vitamin K :
Vitamin K is recognized as the anti haemorrhagic factor
owing to its vital role in blood clotting mechanism.
Functions
Synthesis of blood clotting proteins. Vitamin K is
essential for the activation of prothrombin. This gets converted
208
to thrombin, which in turn activates fibrinogen to form fibrin.
The process of blood clotting occurs as follows:
Injured tissue releases thromboplastin, which catalyses
prothrombin formation. Vitamin K catalyses, conversion of
prothrombin to thrombin. This in turn causes conversion of
fibrinogen to fibrin which forms the clot.
Food Sources:
Dark green leafy vegetables are good sources of vitamin K.
Fruits, tubers, seeds, dairy and meat products contain
Vitamin K.
Requirements :
The ICMR committee considered that no
recommendation is needed for this Vitamin, as the synthesis of
Vitamin K occurs in the lower intestine by the colonic bacteria
and present widely in foods.
Effects of Deficiency:
Primary deficiency arises in infants resulting in delayed
blood clotting and hemorrhage. This is because the new born
babies have a sterile intestinal tract thus lack in the colonic
bacterial colonies which produces Vitamin K. Vitamin K
deficiency does not occur in adults.

Water soluble Vitamins:

Vitamin C (Ascorbic Acid)
The chemical name for Vitamin C is ascorbic acid. It
was discovered in 1747 by the British physician Lind and
demonstrated that citrus fruit juices prevented and cured scurvy.
Functions :
1. Ascorbic Acid is essential for formation of cement
substances and collagen which is found in blood vessels
teeth and bones.
2. It helps in the biosynthesis of non-essential amino acids
(eg) hydroxy proline, tyrosin.
3. It is required for absorption of iron as it reduces ferric to
ferrous form which is easily absorbed.
209
 4. Vitamin C is essential for the formation of collagen a
major structural protein of connective tissues.
5. It is required for normal wound healing because it helps
in the formation of connective tissue.
6. Vitamin C is required for carnitine synthesis which aids
in the transport of fatty acids in the cell.
7. Vitamin C is essential for the synthesis of
norepinephrine a neurotransmitter.
8. It activates hormones (eg) growth hormone, gastrin
releasing peptide, calcitonin, gastrin oxytocin.
9. Drug detoxifying metabolic systems in the body require
Vitamin C for its optimal activity.
10. Vitamin C is an excellent anti-oxidant. It combines with
free radicals oxidizing them to harmless substances that
can be excreted.
Food Sources:
Amla, drumstick leaves, guava, cashew fruit, agathi,
cabbage, bitter gourd, oranges, tomatoes are good sources of
ascorbic acid. Cereals and pulses are poor sources. Vitamin C
content of pulses increases on germination.
Requirements:
The recommended dietary allowances of ICMR for
ascorbic acid is as given in table-21D.

Table 21 D
ICMR Recommended Dietary Allowances for Vitamin C

Group Requirement
mg/day
Adult 40
Pregnant women 40
Lactation 80
Infants 25
Children 40

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 Effects of Deficiency:
Prolonged deficiency of ascorbic acid produces a disease
condition called as ‘ scurvy’ in both infants and adults.
Infantile scurvy:
There is loss of appetite, failure to gain weight,
irritability, palor, defective growth of bones. Haemorrhage
occurs under the skin. There is defective formation of teeth and
gums are swollen. The ends of the ribs become prominent
resulting in beaded appearance called scorbutic rosary.
Adult Scurvy:
1. General manifestation are fever, susceptibility to
infection, and delayed wound healing.
2. Anaemia: Microcytic hypochromic anaemia develops
due to failure of absorption of iron.
3. Gums become spongy and bleed easily. Gums become
swollen and ulcerated.
4. The blood vessels become fragile and porous due to
defective formation of collagen. Joints become swollen
and tender.
5. Clinical symptoms appear when total body pool of
ascorbic acid decreases. Skin becomes rough and dry.
There are small petechial hemorrhages around hair
follicles.
Thiamine
Thiamine is known as Vitamin B1. Deficiency of
thiamine leads to beri – beri. This condition is widely prevalent
among population whose diet contains more of polished cereals.
Functions
1. Thiamine is converted to thiamine pyrophosphate (TPP),
which is an important co enzyme in the carbohydrate
metabolism.
2. It is involved in transmission of nerve impulses across
the cells
3. Thiamine as TPP is an essential cofactor for the
conversion of amino acid tryptophan to niacin.
211
Sources:
Yeast, whole wheat, millets, hand pounded rice,
parboiled rice are good sources of thiamine. The bran contains
most of the thiamine in the cereals. Gingelly seeds, groundnut,
soyabean, cashewnuts, organ meats, pork, liver and eggs supply
thiamine.
Requirements
Thiamine is involved in the carbohydrate metabolism. Its
requirement is related to energy derived from carbohydrate. The
ICMR expert group recommends an allowance of 0.5 mg per
1000 Kcal for adults and for infants 0.3 mg/1000 Kcal is
suggested. The recommended dietary allowance per day is given
in table-21E.
Table 21 E
ICMR Recommended Dietary Allowance For Thiamine Per Day
Group Thiamine requirement mg/day
Man
Sedentary 1.2
Moderate 1.4
Heavy work 1.6
Woman
Sedentary 0.9
Moderate 1.1
Heavy work 1.2
Pregnant woman +0.3
Lactation +0.3 - +0.2
Infants 55 mg/kg – 50 mg/kg
Children (1 – 9 years) 0.6 – 1.2
Boys (10 – 18 years) 1.1 – 1.3
Girls (10 – 18 years) 1.0
Effects of Deficiency
Deficiency of thiamine is associated with low calorie
intake. Severe deficiency of thiamine produces a disease known
as beri – beri.

212
It is manifested as
a. Dry beri – beri
b. Wet beri – beri
c. Infantile beri – beri
a. Dry beri – beri
There is loss of appetite, tingling numbness and burning
sensation in hands and feet. Calf muscles are tender.
Knee and ankle jerks are sluggish.
In later stages complete loss of sensation in hands
and legs occur. It is characterized by foot and waist drop.
Mental depression and confusion occurs.
b. Wet beri – beri
In this case there is enlargement of heart and the
cardiac output is high. Oedema or accumulation of fluid
in legs, face and trunk is observed. palpitations are
marked.
c. Infantile beri – beri
It occurs in first few months of life if the diet of
the mother is deficient in thiamine. Symptoms are
restlessness, sleeplessness, constipation, enlargement of
the heart and breathlessness.
Riboflavin
Riboflavin or Vitamin B2 is the yellow enzyme which is
heat stable unlike other B Vitamins. Riboflavin in the
combined form with proteins form flavo proteins or yellow
enzymes.
This enzyme is of two types FAD – Flavin-di-nucleotide.
FMN- Flavin mono-nucleotide.
a. These substances act as coenzymes in many
biological reactions primarily in oxidation –
reduction, and dehydrogenation reaction
i) Release of energy from glucose, fatty acids
and amino acids.
ii) Conversion of vitamin B6 and folate to active
coenzymes.
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 c) It is essential for the formation of red blood cells
d) It is required for the synthesis of glycogen
Food Sources:
Rich sources are liver, dried yeast, egg, milk , meat, fish,
whole cereals, legumes, and green leafy vegetables.
Requirements
Riboflavin requirement is related to energy intake – 0.6
mg/1000Kcal. The ICMR recommends the following
requirement per day as given in table-21F.

Table 21 F
ICMR Recommended Dietary Allowance for Riboflavin
Group Riboflavin mg/day
Man
Sedentary 1.4
Moderate 1.6
Heavy work 1.9
Woman
Sedentary 1.1
Moderate 1.3
Heavy work 1.5
Pregnant woman +0.2
Lactation +0.3
Infants 65 mg/kg – 60 mg/kg
Children (1 – 9 years) 0.7 – 1.2
Boys (10 – 18 years) 1.3 – 1.6
Girls (10 – 18 years) 1.2
Effects of Deficiency
Riboflavin deficiency is prevalent mainly among the low
income groups particularly the vulnerable group and the elderly
adults. Riboflavin deficiency is characterized by
1. Soreness and burning of the mouth and tongue.

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 2. Lesions at the angles of the mouth called Angular
Stomatitis.
3. The inflammation of the tongue called glossitis

Fig 21.3 Angular Stomatitis (Soreness and ulceration on lips

at the corners of the mouth)
Source : Venkatachalam.P.S and Rebello.R.M. 1996 Nutrition
for Mother and Child. NIN; ICMR.
4. Dry chapped appearance of the lip with ulcers termed
cheilosis.
5. The skin becomes dry and results in seborehoeic
dermatitis.
6. Photophobia, lacrimation, burning sensation of the
eyes and visual fatigue.
7. Decreased motor co-ordination
8. Normocytic anaemia
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Niacin
Niacin or Nicotinamide (amide form) is required by all
the cells of our body.
Like thiamine and riboflavin it plays a vital role in the
release of energy from carbohydrates , protein, fat and alcohol.
Functions
1) Nicotinamide is essential for tissue metabolism. The
active forms of nicotinanide are NAD – Nicotinamide
adenine dinucleotide and NADP - Nicotinamide adenine
dinucleotide phosphate.
2) NAD and NADP are involved as coenzymes in large
number of reversible oxidation reduction reactions.
3) Nicotinic acid enhances stomach secretion
4) NAD is involved in catabolic reactions and NADP is
involved in anabolic reaction in our body.
Food Sources
Dried yeast, liver, rice polishing, peanut, whole cereals,
legumes, meat, fish, are good sources.
Tryptophan present in dietary protein is converted to
niacin in humans. 60 mg of tryptophan yields 1 mg of niacin.
Requirements :
ICMR recommended dietary allowance of Niacin per
day is given in table 21G.
Table 21 G
ICMR Recommended Dietary Allowance for Niacin
Group Niacin requirement mg/day
Man 16 – 21
Woman 12 – 16
Pregnant woman +2
Lactation +4
Infants 710 mg/kg – 650 mg/kg
Children (1 – 9 years) 8 – 13
Boys (10 – 18 years) 15 – 17
Girls (10 – 18 years) 13 – 14

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Effects of Deficiency
Deficiency of nicotinic acid causes a disease known as
pellagra. It is characterized by three D's à Dermatitis,
Diarrhoea and Dementia.
1. Dermatitis – Name pellagra comes from pelle-skin and
agra-rough. Marked changes occur in the skin especially
in the skin exposed to sun and friction areas like elbows,
surfaces of arms, knees.
Lesions are symmetrically distributed, in the affected
parts. At first there is reddening, thickening and
pigmentation of the skin.
Later on there is exfoliation leading to ultimately
parchment of skin – butterfly like appearance.
2. Diarrhoea – Diarrhoea enhances the deficiency state.
There are structural and absorptive defects in the small
intestine. Tongue appears raw, and mucous membrane of
the tongue is inflammed.
3. Dementia – There is irritability, depression, poor
concentration and loss of memory. Delirium is a
common mental disturbance.
Folic Acid
Folic acid was first extracted from dark green leafy
vegetables. It forms yellow crystals and is a conjugated
substance made up of three acids namely pteroic, para amino
benzoic acid and glutamic acid.
Functions
1. Folic acid coenzyme is essential in bringing about
transferring single carbon units for many
interconversions. A number of key compounds are
formed by these reactions like (i) Purines which are
essential constituents of living cells.
(ii) Thymine – this essential compound forms a key part
of DNA.
(iii) the formation of haem group of haemoglobin.
2. The conversion of phenylalanine into tyrosin.
217
Food Sources:
Green leafy vegetables, liver, kidney, gingelly seeds,
cluster beans, are rich sources of folic acid.
Requirements
The recommended dietary allowances of Folic acid by
ICMR are given in table-21H .

Table 21 H
ICMR Recommended Dietary Allowance for Folic acid
Group Folic acid mg/day
Man 100
Woman 100
Pregnant woman 400
Lactation 150
Infants 25
Children (1 – 9 years) 30 – 60
Boys and girls (10 – 18 years) 70 – 100

Deficiency
1. Simple folate deficiency results in the bone marrow
producing immature cells (megaloblasts cells) and few
matured red blood cells. This results in reduced oxygen –
carrying capacity causing anaemia termed -
Megaloblastic anaemia.
2. Folate deficiency during pregnancy causes neural tube
disorders of the foetus.
3. Folate deficiency impairs the ability of the immune
system to fight infection.
Pyridoxine (B6)
Pyridoxine is unique among B – complex Vitamins in
that it functions primarily in protein metabolism.
Pyridoxine denotes related substances such as
Pyridoxine, Pyridoxal and Pyridoxamine are three forms in
which it is present in our body.
218
Functions
Vitamin B6 in the form of pyridoxal phosphate functions as a
co-enzyme in many biological reactions
1. Pyridoxine is essential for the process of
a. Transamination : transfer of amino group from one
aminoacid to another.
b. Deamination : Removal of the amino group
c. Decarboxylation: Removal of the carboxyl group
2. Vitamin B6 is involved in several biochemical steps for
the conversion of the amino acid tryptophan to niacin
3. It aids in the formation of elastin, synthesis of
messenager RNA and haem part of haemoglobin.
4. It aids in the conversion of linoleic acid to arachidonic
acid.
5. In the carbohydrate metabolism it aids in the release of
glycogen from liver and muscle.
Food sources:
Meat, pulses and wheat are rich sources. Other Cereals
are fair sources of this vitamin. Fruits and vegetables are poor
sources. Cooking and processing of food causes loss of this
vitamin.
Requirement:The ICMR recommended dietary allowance for
pyridoxine is given in table-21 I.
Table 21 I
The ICMR Recommended Dietary Allowance for Pyridoxine
Group Pyridoxine mg/day
Adults 2.0
Pregnant woman 2.5
Lactation 2.5
Infants 0.1 – 0.4
Children (1 – 9 years) 0.9 – 1.6
Boys and girls (10 – 18 years) 1.6 – 2.0
Deficiency
Vitamin B6 deficiency leads to abnormalities in protein
metabolism which is manifested as poor growth, convulsions,
219
anaemia, decreased antibody formation and skin lesions. Severe
deficiency leads to microcytic hypochromic anaemia.
Symptoms such as weakness, nervousness, irritability,
insomnia and difficulty in walking is predominant.

Vitamin B12 (Cyanocobalamin)

Until 1926, pernicious anaemia was a fatal disease of unknown
origin with an unknown cure. In 1926 Minot and Murphy found
that pernicious anaemia could be cured by feeding a patient
atleast 0.3 kg of raw liver per day.
Also in 1926 Castle noted that patients with pernicious
anaemia had a low level of gastric secretion. He suggested that
the anti-pernicious anaemia factor had two components.; an
‘ extrinsic factor’ found in food and an ‘ intrinsic factor’ within
normal gastric secretions. The extrinsic factor is now known as
vitamin B12 – cobalamine.
Functions :
Vitamin B12 is necessary for normal growth and
maintenance of healthy nervous tissue and normal blood
formation.
Vitamin B12 is involved in DNA synthesis and thus in
cell replication.
In the bone marrow the Vitamin B12 co-enzymes are
essential for the formation of red blood cells.
It facilitates the formation of folate co-enzymes needed
for nucleic acid synthesis.
Vitamin B12 is also required for the synthesis of myelin
sheath that surrounds the nerve fiber.
Food sources
Vitamin B12 is present only in foods of animal origin.
Liver sheep, shrimp, mutton , egg, milk are good sources of
Vitamin B12.Vitamin B12 is synthesized by the colonic bacteria.
Requirements :
The recommended dietary allowance prescribed by
ICMR for B12 are given in table 21J.
220
 Table 21J
The ICMR Recommended Dietary Allowance for
VitaminB12
Group Vitamin B12 mg/per day
Man 1.0
Woman 1.0
Pregnancy 1.0
Lactation 1.5
Infants 0.2
Children boys and girls 0.2 – 1.0

Deficiency
Pernicious amaemia is the major problem arising from
an inadequate amount of vitamin B12.
Pernicious amaemia is a condition characterized by very
large, immature red blood cells with normal amounts of
haemoglobin.

QUESTIONS

Part B
Write short answers:
1. What are macro minerals and micro minerals give
example?
2. What is a provitamins?

3. What is hypervitaminosis?
4. List the functions of calcium and phosphoros?
5. What are goitrogens?
6. Discuss the functions of Iron?

221
 7. Discuss the role of Vitamin A in the visual process?
8. Why is Vitamin E called the anti-sterility vitamin?
9. Explain the process of clotting of blood?
10. List any two differents between fat soluble and water
soluble vitamins?
Part C
Write detailed answers:
1. Discuss Vitamin A,D,E,K under the following heads
a. Functions
b. Requirements

c. Sources
d. Effects of deficiency
2. Discuss effects of deficiency of the B-complex vitamins?
3. Explain in detail the functions and deficiency of
Vitamin C?

222
 22. WATER
Water is vital for human existence. We can live with out
food for extended periods of time, but without water will result
in death.
Water is colourless, calorie less compound of hydrogen
and oxygen that virtually every cell in the body needs to survive.
Water is closer being a universal solvent than any other
compound.
Water is the largest single compound of the body and it
is distributed as follows.
Distribution of water in the body
Water

Intracellular water Extracellular water

(Present inside the cell) (present outside the cell)

Intravascular Extravascular
(Present as plasma of blood) (Present outside the blood
vessels)
(i) Interstitial fluid
( between the cells)
(ii) Lymph
(iii) Water present in brain,
aqueous humor of the
eye, pericardium,
pleural cavities.

223
 Total body water content is mainly determined by total
amount of salt in the body. Salt and water concentration in the
body is controlled by the kidneys.

22.1 FUNCTIONS OF WATER

1. It is an essential constituent of all the cells of the body
and the internal environment.
2. Serves as a transport medium by which most of the
nutrients pass into the cells and removes excretory
products.
3. Water is a medium for most biochemical reactions
within the body and sometimes a reactant.
4. It is a valuable solvent in which various substances such
as electrolytes, non – electrolytes, hormones, enzymes,
vitamins are carried from one place to another.
5. Plays a vital role in the maintenance of body
temperature. Heat is produced when food is burnt for
energy. Body temperature must be kept at 80º - 108º
Fahrenheit for higher or lower body temperature will
cause death. Body heat is lost through the skin, lungs,
urine and faeces.
6. It forms a part of fluids in body tissues; (eg) the amniotic
fluid surrounds and protects the foetus during pregnancy.
7. Saliva is about 99.5 percent water. In healthy individuals
it makes swallowing easier by moistening the food.
8. Water helps in maintaining the form and texture of the
tissues.
9. Water is essential for the maintenance of acid base and
electrolyte balance. It should be noted that pure water
consists of hydrogen ion (H+) and hydroxyl ion (OH-).
Substances dissolve in water as ions with positive and
negative charge. They are called electrolytes. The common
electrolytes in our body are sodium, potassium and chloride.
Changes in electrolyte balance causes accumulation or
depletion of water in intracellular and extra cellular fluid.
224
 The balance between the positively and negatively charged
ions is essential for water flow and maintain osmolarity between
the cells. This is called electrolyte balance.
Acid base balance is the dynamic state of equilibrium of
hydrogen ion concentration. When pH falls below 7 it is termed
acidity and when it increases above 7 it is termed alkalinity.
Extremes of both cases results in death. The pH of the body
should be maintained near neutrality. Enzymatic action depends
on the pH. The digestion, absorption and utilization of nutrients
are dependent on pH. Most body fluids are near neutral with the
exception of gastric juice.
The pH value of some solutions are given below:
Acid
0 – Hydrochloric acid
1
2 – Gastric juice
3 – Vinegar, orange juice
4 – Grapes
5 – Bread, coffee
Neutral 6 – Urine
7 – Pure water, eggs, blood
8 – Sea water
14 – Sodium hydroxide
Alkali
10. Water forms good source of macro minerals like Calcium,
Magnesium, Fluoride, Iron and Iodine.
22.2 REQUIREMENTS
Requirements of water varies with climate, dietary
constituents, activities and surface area of the body. As a rule a
person should take enough water to excrete about 1200 –1500
ml of urine per day. In tropics because of greater water loss
through perspiration increased water intake is required to
maintain urine volume. Normal intake of water ranges between
8 – 10 glasses per day.

225
 Fig 22.1 Intake of water and its output

Source : Susan.R.Holman,1987. Essentials of Nutrition for

the Health Professionals.

Daily Water Input

In tropical countries like India the daily water input amounts to
2400 – 3000 ml of water through food, as fluid drinks and as
metabolic water.

226
1. As fluid drinks – water, tea, coffee,
milk soups 1500 - 1750 ml
2. Water intake through solid food 600 - 900 ml
3. Oxidation of carbohydrate, fat, 300 - 350 ml
proteins (metabolic water)
Total 2400 – 3000 ml

22.2.2 Daily output of water

1. Urine 1200 – 1500 ml à (kidney)
2. Perspiration 700 – 900 ml à (Skin)
3. Respiration 400 ml à (lung)
4. Faeces 100 – 200 ml à (intestine)
Total 2400 – 3000 ml
Therefore the water intake and output is fairly kept
constant. This is called water balance. The average adult
metabolises 2.5 – 3.00 litres of water and a constant balance is
maintained between intake and output. Inadequate water intake
disturbs water equilibrium resulting in decreased urinary output,
thereby causing changes in Extra Cellular Fluid (ECF) and Intra
Cellular Fluid (ICF). The water equilibrium is maintained by
kidneys, lungs, intestine and pituitary gland. The water balance
coordinates with both electrolyte and acid base balance.

22.3 CAUSES AND EFFECTS OF DEHYDRATION

Causes :
When water is constantly lost from the body as in severe
vomiting, diarrhoea, excessive sweating or excessive urine
formation due to treatment with diuretics, the total water content
of the body is reduced. Extra cellular and intra cellular fluid
decreases leading to dehydration.
Effects of dehydration
1. Tongue is dry.
2. Pinch test is done by raising and releasing the skin. Slow
return of skin to original position indicates decreased
ECF.
227
 3. Decrease in plasma volume reduces cardiac output and
may lead to cardiac failure.

22.4 PREVENTION OF DEHYDRATION

Dehydration can be prevented by taking sufficient
amounts of water as fluids. The correction of dehydration is
called rehydration.

Oral rehydration therapy

It is the administration of fluid to prevent or correct
dehydration.
22.4.1 Oral rehydration salt
WHO, UNICEF formula consist of the NaCl – 3.5 g,
NaHCO3 – 2.5 g, KCl – 1.5g and glucose - 20 g to be dissolved
in one litre of potable drinking water.

The Glucose present aids in the absorption of sodium

chloride and potassium chloride apart from giving energy. This
mixture is administered through the oral route at frequent
intervals until the normal state is attained.
Potable water is that water which is safe and wholesome. It
should be:
a. free from pathogenic agents
b. free from harmful chemical substance
c. pleasant to taste; free from colour and odour
d. usable for domestic purpose.

228
 QUESTIONS

Part A
Fill in the blanks:
1) Administration of water or fluid orally to correct
dehydration is called ___________

2) Water which is safe and wholesome is called

___________ water
3) The normal intake of water amounts to _________
4) The equilibrium between water intake and output is
called _________

Part B
Write short answers:
1) How is water distributed in the body?
2) Enumerate the effects of dehydration and give the
composition of oral rehydration salt.
3) Write short notes on Electrolyte balance and acid – base
balance.

Part C
Write detailed answers:
Enumerate the functions of water in our body.

229
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