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    09 Linear Forced Through Zero Calib PDF

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    BryanMojalottMorote

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    09 Linear Forced Through Zero Calib PDF

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    98 views13 pages

    09 Linear Forced Through Zero Calib PDF

    Uploaded by

    BryanMojalottMorote

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 13

    Linear Forced Through

    Zero

    Definition
    A calibration curve defined using this
    equation is forced to go through zero
    intensity and zero concentration. This
    It is often tempting to exclude the intercept, a, from the model because a zero stimulus on the x-axis should lead to a zero response on the y-axis.
    However, the correct procedure is to fit the full model and test for the significance of the intercept term
    calibration is established by assuming that
    the relationship between concentration and
    intensity is linear.
    Ref: WinLab32 Help copyright 1999 - 2004 by
    PerkinElmer, Inc. All rights reserved.

    Linear Forced Through Zero


    It is often tempting to exclude the intercept, a,
    from the model because a zero stimulus on the
    x-axis should lead to a zero response on the yIt is often tempting to exclude the intercept, a, from the model because a zero stimulus on the x-axis should lead to a zero response on the y-axis.
    However, the correct procedure is to fit the full model and test for the significance of the intercept term
    axis. However, the correct procedure is to fit
    the full model and test for the significance of
    the intercept term .
    http://www.itl.nist.gov/div898/handbook/mpc/section3/
    mpc361.htm Section 2.3.6.1

    Linear Forced Through Zero


    Do not include the origin (0,0) as an extra
    calibration point.
    Forcing the curve through zero is not the same
    as including the origin as a fictitious point in
    the calibration.
    If the curve is forced through zero, the
    intercept is set to 0 before the regression is
    calculated, thereby setting the bias to favor the
    low end of the calibration range by pivoting
    the function around the origin to find the best
    fit and resulting in one less degree of freedom.
    Ref: SW-846, Method 8000C, Section 11.5.2.1

    Linear Forced Through Zero


    Do not include the origin (0,0) as an extra
    calibration point.
    Forcing the curve through zero is not the same
    as including the origin as a fictitious point in
    the calibration.
    If the curve is forced through zero, the
    intercept is set to 0 before the regression is
    calculated, thereby setting the bias to favor the
    low end of the calibration range by pivoting
    the function around the origin to find the best
    fit and resulting in one less degree of freedom.
    Ref: SW-846, Method 8000C, Section 11.5.2.1

    Linear Forced Through Zero


    Do not include the origin (0,0) as an extra
    calibration point.
    Forcing the curve through zero is not the same
    as including the origin as a fictitious point in
    the calibration.
    If the curve is forced through zero, the
    intercept is set to 0 before the regression is
    calculated, thereby setting the bias to favor the
    low end of the calibration range by pivoting
    the function around the origin to find the best
    fit and resulting in one less degree of freedom.
    Ref: SW-846, Method 8000C, Section 11.5.2.1

    Linear Forced Through Zero


    It may be appropriate to force the regression
    through zero for some calibrations.
    However, the use of a linear regression or
    forcing the regression through zero may NOT be
    used as a rationale for reporting results below
    the calibration range demonstrated by the
    analysis of the standards.
    If it is necessary to report results at lower
    concentrations, then the analyst should run a
    calibration that reaches those lower
    concentrations.
    Ref: SW-846, Method 8000C, Section 11.5.2.1

    Linear Forced Through Zero


    It may be appropriate to force the regression
    though zero for some calibrations.
    However, the use of a linear regression or
    forcing the regression through zero may NOT be
    used as a rationale for reporting results below
    the calibration range demonstrated by the
    analysis of the standards.
    If it is necessary to report results at lower
    concentrations, then the analyst should run a
    calibration that reaches those lower
    concentrations.
    Ref: SW-846, Method 8000C, Section 11.5.2.1

    Linear Forced Through Zero


    It may be appropriate to force the regression
    though zero for some calibrations.
    However, the use of a linear regression or
    forcing the regression through zero may NOT be
    used as a rationale for reporting results below
    the calibration range demonstrated by the
    analysis of the standards.
    If it is necessary to report results at lower
    concentrations, then the analyst should run a
    calibration that reaches those lower
    concentrations.
    Ref: SW-846, Method 8000C, Section 11.5.2.1

    Linear Regression Equations


    Forced Through Zero
    Calculations for a linear least square
    regression that is forced through zero are
    performed using the equations as
    described for a linear least square
    regression. To determine the slope and
    intercept for a curve forced through zero
    all concentration and response values are
    entered as determined and the negative
    integers of these concentrations and
    responses are also entered for each level.

    Benefits
    Simple; calibration can be represented
    by y = mx.
    Is correct when the origin (zero, zero) is
    within the error of the measurement.
    Ref:Roland Caulcutt and Richard Boddy, 1983,
    "Statistics for Analytical Chemists," Chapman
    and Hall, New York, ISBN 0-412-23730-X, p 91.

    Disadvantages
    Not best fit to data unless slope is the
    same at all points.
    Incorrect when points have responses
    offset from zero (e.g., high blank).
    Ref: Roland Caulcutt and Richard Boddy,
    1983, "Statistics for Analytical Chemists,"
    Chapman and Hall, New York, ISBN 0-41223730-X, p 91.

    EnviroQuant Flaw With the r2 Determination of Linear (0,0)

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