10 Pepsin On Egg White
10 Pepsin On Egg White
10 Pepsin On Egg White
01
Contents
Results
Enzymes 10.02
5 cm3
egg-white
suspension
boiled pepsin
add 1cm3 pepsin
to tubes 1 and 3
approx 40C
Enzymes 10.03
Outline In the presence of hydrochloric acid, pepsin turns a cloudy suspension of egg-white into
a clear solution. Controls are conducted with hydrochloric acid by itself, pepsin by itself and
boiled pepsin with hydrochloric acid.
Prior knowledge The interpretation of results will be easier if the student knows already that
egg-white suspension consists of solid particles of albumen, suspended in water; enzymes are
inactivated by boiling, conditions in the stomach are acid.
Advance preparation and materials-per group
1% egg-white suspension * 25 cm
1% pepsin solution 10 cm3
hydrochloric acid
(bench strength, e.g. 10% or 2M) 5 cm3
Apparatus-per group
test-tube rack and 5 test-tubes
4 labels or spirit marker
Bunsen burner
test-tube holder