LDH P
LDH P
LDH P
DGKC-(5+1)
PROCEDURE
164
164
164
164
164
Cat.No
216
200
203
201 HIT I
202 HIT II
7
5
4
4
4
x
x
x
x
x
Package Size
10 mL
2 x 7 mL
20 mL
1 x 20 mL
100 mL 4 x 20 mL
50 mL
2 x 20 mL
100 mL 4 x 20 mL
METHOD
Enzymatic, Kinetic
PRINCIPLE
LDH
Pyruvate + NADH > Lactate + NAD+
REAGENTS
COMPOSITION (Concentrations in the test) :
Reagent R1:
TRIS-Buffer pH 7.5
50mmol/L
Pyruvate
0.6mmol/L
Sodium Azide
0.09%
Reagent R2 (Starter reagent):
NADH
0.18mmol/L
STABILITY
The sealed reagents are stable up to the indicated
expiry date if stored at +2 to +8C.
PREPARATION
R1 and R2 are ready for use
Stability after opening :
at least 3 months
at +2 to +8 C , if contamination is avoided
Working Reagent (WKR)
Mix 5 parts of reagent R1 with 1 part of R2
Stability: at +2 to +8 C: 8 days
at +18 to +25 C: 2 days
Wavelength :
Light path:
Temperature :
334/340/365nm
1cm
37 C
Start with R2
1000L
20L
200L
CALCULATION
with Factor
A /min x Factor = LDH-Activity [U/l]
Factor for Serumstart:
340 nm
8095
334 nm
8250
365 nm
15000
Factor for Start with reagent R2 :
340 nm
9683
334 nm
9870
365 nm
17940
SAMPLE MATERIAL
Serum, heparin-/EDTA-plasma
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REFERENCE VALUES
Sensitivity
The detection limit is 2,42 U/L
BIBLIOGRAPHY
Expected range:
Adults: 240-480 U/L
(4.00-8.00kat/L)
- Specificity
When a control value of known concentration is
assayed, the assay value falls within the range of
<10% of the known concentration.
- Precision
When the indicated number of samples is
assayed, the following CV values are obtained:
SYMBOLS USED
Within run
n = 11
Sample 1
Sample 2
Sample 3
Mean
[U/L]
300
491
368
SD
[U/L]
4.56
2.25
4.29
CV
[%]
1.52
0.46
1.17
Day to day
n = 11
Sample 1
Sample 2
Sample 3
Mean
[U/L]
303
491
352
SD
[U/L]
4.56
2.95
3.65
CV
[%]
1.50
0.60
1.04
Batch Code
Use by
Temperature limitation
Correlation
A comparative study has been performed between
the Greiner method and another commercial
reagent on 29 human serum samples. The
parameters of linear regression are as follows:
Correlation coefficient
Linear regression:
r = 0.9956
y = 1.019 x + 2.653 U/L
INTERFERENCES
-Ascorbic Acid:
-Triglycerides:
-Bilirubin:
-Hemoglobin:
No interference up to 50 mg/L
No interference up to 1000 mg/dL
no interference up to 40 mg / dL
Interference through LDH from
the erythrocytes
08/2010//rev03/2014
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