MW-12A Microplate Washer

Service Manual
 2007-2010 Shenzhen Mindray Bio-Medical Electronics Co., Ltd. All rights
Reserved.

For this Operators Manual, the issue date is 2010-04.

Intellectual Property Statement

SHENZHEN MINDRAY BIO-MEDICAL ELECTRONICS CO., LTD. (hereinafter called
Mindray) owns the intellectual property rights to this Mindray product and this
manual. This manual may refer to information protected by copyright or patents and
does not convey any license under the patent rights or copyright of Mindray, or of
others.

Mindray intends to maintain the contents of this manual as confidential information.

Disclosure of the information in this manual in any manner whatsoever without the
written permission of Mindray is strictly forbidden.

Release, amendment, reproduction, distribution, rental, adaptation, translation or any

other derivative work of this manual in any manner whatsoever without the written
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, , , , BeneView,
WATO, BeneHeart, are the trademarks, registered or otherwise, of Mindray in
China and other countries. All other trademarks that appear in this manual are
used only for informational or editorial purposes. They are the property of their
respective owners.

Responsibility on the Manufacturer Party

Contents of this manual are subject to changes without prior notice.
All information contained in this manual is believed to be correct. Mindray shall not
be liable for errors contained herein nor for incidental or consequential damages in
connection with the furnishing, performance, or use of this manual.
Mindray is responsible for safety, reliability and performance of this product only in
the condition that:

all installation operations, expansions, changes, modifications and repairs of
this product are conducted by Mindray authorized personnel;

the electrical installation of the relevant room complies with the applicable
national and local requirements;

the product is used in accordance with the instructions for use.

WARNING:
It is important for the hospital or organization that employs this
equipment to carry out a reasonable service/maintenance plan.
Neglect of this may result in machine breakdown or injury of human
health.

i
 NOTE:
This equipment is to be operated only by medical professionals trained
and authorized by Mindray or Mindray-authorized distributors.

Warranty
THIS WARRANTY IS EXCLUSIVE AND IS IN LIEU OF ALL OTHER WARRANTIES,
EXPRESSED OR IMPLIED, INCLUDING WARRANTIES OF MERCHANTABILITY
OR FITNESS FOR ANY PARTICULAR PURPOSE.

Exemptions
Mindray's obligation or liability under this warranty does not include any
transportation or other charges or liability for direct, indirect or consequential
damages or delay resulting from the improper use or application of the product or the
use of parts or accessories not approved by Mindray or repairs by people other than
Mindray authorized personnel.
This warranty shall not extend to:

any Mindray product which has been subjected to misuse, negligence or
accident;

any Mindray product from which Mindray's original serial number tag or product
identification markings have been altered or removed;

any product of any other manufacturer.

Return Policy
Return Procedure
In the event that it becomes necessary to return this product or part of this product to
Mindray, the following procedure should be followed:
1 Obtain return authorization: Contact the Mindray Service Department and
obtain a Customer Service Authorization (Mindray) number. The Mindray
number must appear on the outside of the shipping container. Returned
shipments will not be accepted if the Mindray number is not clearly visible.
Please provide the model number, serial number, and a brief description of
the reason for return.
2 Freight policy: The customer is responsible for freight charges when this
product is shipped to Mindray for service (this includes customs charges).
3 Return address: Please send the part(s) or equipment to the address offered
by Customer Service department

Company Contact
Manufacturer: Shenzhen Mindray Bio-Medical Electronics Co., Ltd.
Address: Mindray Building, Keji 12th Road South, Hi-tech Industrial Park,
Nanshan, Shenzhen 518057, P.R.China
Tel: +86 755 26582479 26582888
Fax: +86 755 26582934 26582500

ii
 Preface

Who Should Read This Manual

The manual is written for service professionals authorized by Mindray.

Conventions Used in This Manual

Safety Symbols
This chart explains the symbols that may be used in this manual.
When you see Then
Read the statement following the symbol. The
WARNING: statement is alerting you to an operating
hazard that can cause personal injury.

Read the statement following the symbol. The

BIOHAZARD: statement is alerting you to a potentially
biohazardous condition.

Read the statement following the symbol. The

CAUTION: statement is alerting you to a possibility of
system damage or unreliable results.

Read the statement following the symbol. The

NOTE: statement is alerting you to information that
requires your attention.

EC Representative
Name: Shanghai International Holding Corp. GmbH (Europe)
Address: Eiffestrae 80, 20537 Hamburg Germany
Phone: 0049-40-2513175
Fax: 0049-40-255726

Preface 1
 Contents
Preface............................................................................................................... 1
Who Should Read This Manual.......................................................................................... 1
Conventions Used in This Manual...................................................................................... 1

1 System Features ...................................................................................1-1

2 System Installation ...............................................................................2-1

3 System Description ..............................................................................3-1

3.1 Workflow ............................................................................................................... 3-3
3.2 Components ......................................................................................................... 3-3
3.2.1 Plate Carrier Assembly ............................................................................ 3-4
3.2.2 Manifold Module ...................................................................................... 3-4
3.2.3 Bottom Plate Assembly............................................................................ 3-6
3.2.4 Fluid Path................................................................................................. 3-6

4 Hardware Boards ..................................................................................4-1

4.1 Main Control Board............................................................................................... 4-3
4.1.1 Main Control Unit ..................................................................................... 4-4
4.1.2 Driver Unit................................................................................................ 4-4
4.2 Keypad Board ....................................................................................................... 4-4
4.3 Power Supply Board ............................................................................................. 4-4
4.4 LCD Module.......................................................................................................... 4-5
4.5 Connecting Diagram ............................................................................................. 4-5
4.6 Interface Definition................................................................................................ 4-6

5 Maintenance and Service .....................................................................5-1

5.1 Maintenance ......................................................................................................... 5-3
5.1.1 Daily Maintenance ................................................................................... 5-3
5.1.2 Weekly Maintenance ............................................................................... 5-3
5.1.3 Monthly Maintenance............................................................................... 5-3
5.2 Service.................................................................................................................. 5-4
5.2.1 Unclogging Needle .................................................................................. 5-4
5.2.2 Replacing Tubing for Three-position Valve.............................................. 5-4
5.2.3 Replacing Tubing for Two-position Valve................................................. 5-5
5.2.4 Troubleshooting ....................................................................................... 5-6
5.3 Software Upgrade............................................................................................... 5-10

Contents I
1 System Features

System Features 1-1

1-2 System Features
 1 System Features

Specifications

Dimension: 400 mm330 mm145 mm (LDH)

Weight: about 7.2kg

Power supply: 100-240V~, 50/60Hz

Power: 100VA max

Cleaning Unit

Dispense module: Two kinds of wash solution, Wash1 and Wash2; The
maximum volume of each wash solution bottle is 2L; Solution is dispensed by a
fluid pump; The dispense volume is 50-3000l; A three-position valve controls
the switch between two kinds of wash solution; A two-position valve controls the
fluid path of the dispense module.

Waste module: Buffer and Waste bottles have been equipped with the system;
Waste level can be detected; The maximum volume of each bottle is 2L;
Solution is absorbed by a vacuum pump.

Manifold: Separated dispense needles and aspirate needles; 8-/12-channel;
Bump detection

Tubing: Silicone tube used with valves must be replaced periodically.

Connector: Easy to be connected

Moving Unit

Vertical moving module: Drives the manifold to move vertically.

Horizontal moving module: Drives the plate carrier to move horizontally.

Others

The system is operated via the keypad on the front panel.

Display: LCD, 192*64

Interface: RS-232

System Features 1-3

2 System Installation

System Installation 2-1

2-2 System Installation
 2 System Installation

1 Ensure the installation field in the hospital is available. Refer to the Operation
Manual for installation space, power supply, environmental requirements, etc.
2 Go to the installation site. Check the delivery list and unpack the package.
3 Move the system in place.
4 Install the manifold (8-channel or 12-channel) required by the user.
5 Connect Wash1, Wash2, Waste and Buffer bottles.
6 Connect the power cable and place the power switch to ON. The system will
initialize and, after completing the self-check and rinsing tubing, display the
main interface.
7 Place the power switch to OFF after ensuring the system can work properly.
8 Train users.
Can the user complete routine operations? Yes No
Does the user know how to maintain the system? Yes No
Does the user know how to clean the system? Yes No
Does the user know how to correct common errors? Yes No
Does the user know how to replace tubing for valves? Yes No

System Installation 2-3

 3 System Description

3.1 Workflow ............................................................................................................... 3-3

3.2 Components ......................................................................................................... 3-3
3.2.1 Plate Carrier Assembly ............................................................................ 3-4
3.2.2 Manifold Module ...................................................................................... 3-4
3.2.3 Bottom Plate Assembly............................................................................ 3-6
3.2.4 Fluid Path................................................................................................. 3-6

System Description 3-1

3-2 System Description
 3 System Description

3.1 Workflow
A typical workflow of the system is shown as the figure below.

Wash Completed
Parameter Setup

Microplate Wash

Tubing Rinse
All
Initialization

Shutdown
Operation
Position
Start

Complete

1 Clear the Waste bottle. Add wash solution into Wash1 and Wash2 bottles.
Connect the Wash1, Wash2, Waste and Buffer bottles to the system
properly. Fill the Distilled Water bottle with distilled water or deionized water
for use in washing.
2 Switch on the power supply and the system initializes. The initialization,
which includes tubing rinse, self-check, mechanical parts reset, etc, takes
about 20 seconds.
3 After the initialization, put the microplate on the plate carrier, select the
manifold, and then adjust positions.
4 After adjusting positions, set parameters, such as program number, wash
solution, wash mode, bottom wash, crosswise aspiration, soak time, shaking
time, aspiration time, cycle times, etc.
5 Select a program, select pre-rinse as needed, and then set the row number.
After that, start washing the microplate.
6 Select other microplate wash as needed.
7 After all wash operation is completed, disconnect Wash2 from the system,
and connect Distilled Water bottle to the system through the connector
which connects Wash2 and the system before. Run the tubing rinse program
at least once. After that, shut down the system.

3.2 Components
The system mainly consists of the plate carrier assembly, the manifold module, the
bottom plate assembly and the fluid system.

System Description 3-3

3.2.1 Plate Carrier Assembly
The plate carrier assembly holds microplates and moves them horizontally to wash
position. It consists of the plate carrier, the guide shaft, the belt, etc.

The motor on the bottom plate, which drives the belt, is located near one end of the
guide bar and the roller wheel near the other. The horizontal home position sensor,
which is at the same end with the roller wheel, helps to control the reset of plate
carrier in the horizontal direction. The spring sheet on the plate carrier secures the
microplate to avoid shaking during the wash.

3.2.2 Manifold Module

The manifold module supports the manifold and moves it vertically to reach specified
positions. The module consists of the manifold assembly, the support assembly and
the base assembly.

3.2.2.1 Manifold Assembly

The manifold assembly has two types, 12-channel and 8-channel. The 12-channel is
the default. Users can select appropriate manifolds according to their needs.
Dispense and waste connectors on one side of the manifold connect dispense tubing
and waste tubing respectively. Screws on the other side secure the manifold to the
support assembly and form the bump detection circuit.

3-4 System Description

3.2.2.2 Support Assembly
The support assembly, which consists of a metal plate and a non-metal one,
supports the manifold assembly. The metal plate is the frame and the non-metal one
provides insulation for the bump detection circuit. The conducting wire between the
two plates is connected to the two electrodes fixed on the non-metal plate and forms
the bump detection circuit with the screws on the manifold and the screw connection
sheet.

3.2.2.3 Base Assembly

The base assembly serves as the base of the manifold module, fixes the driving
motor, and guides and locates the sliding block. The vertical home position sensor
on the top of the base assembly detects the vertical moving status of manifold and
helps to control the manifold reset.

System Description 3-5

3.2.3 Bottom Plate Assembly
The bottom plate assembly supports other assemblies, parts, etc. The vacuum pump,
fluid pump, valves, power supply unit, main control board and motors are located on
the bottom plate. Power supply input interface and fluid path connectors are located
on the rear panel.

3.2.4 Fluid Path

The fluid path dispenses wash solution and collects waste. Two kinds of wash
solution are supported.

3-6 System Description

3.2.4.1 Wash Solution Dispensing
The wash solution dispensing path consists of the fluid pump, three-position valve,
two-position valve, wash solution bottles and related tubing and connectors. The
fluid pump provides power for dispensing. The three-position valve performs the
switch between the two kinds of wash solution. The two-position valve can shut off
the path to avoid solution leakage. Wash1 and Wash2 bottles provide wash solution,
which should be added as necessary.

During the wash, the wash solution is powered by the fluid pump and goes from
wash solution bottles to needles through the dispensing tubing and finally reaches
wells.

3.2.4.2 Waste Collection

The waste collection path is powered by the vacuum pump to collect waste. The
Waste and Buffer bottles work together to collect waste into the Waste bottle and to
prevent waste from entering the vacuum pump.

During the collection, the manifold moves downward and absorb needles suck waste
near the bottom of wells.

System Description 3-7

 4 Hardware Boards

4.1 Main Control Board............................................................................................... 4-3

4.1.1 Main Control Unit ..................................................................................... 4-4
4.1.2 Driver Unit................................................................................................ 4-4
4.2 Keypad Board ....................................................................................................... 4-4
4.3 Power Supply Board ............................................................................................. 4-4
4.4 LCD Module.......................................................................................................... 4-5
4.5 Connecting Diagram ............................................................................................. 4-5
4.6 Interface Definition................................................................................................ 4-6

Hardware Boards 4-1

4-2 Hardware Boards
 4 Hardware Boards

The hardware system consists of the following units and boards:

Main control unit: main control board

Driver unit: main control board

Keypad unit: keypad board

Display unit: LCD module

Power supply unit: power supply board

Hardware System

Main Control Power Supply

Driver Unit Display Unit Keypad Unit
Unit Unit

Main Control Power Supply

LCD Module Keypad Board
Board Board

4.1 Main Control Board

Functions of the main control board is shown as the figure below.

Power Supply Board

+ 24V/+12V/+ 5V
Power Supply
Main Control Board

Moving Parts Driver Main Control Unit Pump and Valve Driver

Pump and Driver of 2 Pumps

Step Motor
Driver of 2 Step Motors Valve and 2 Valves
Interface
Interface
Position Waste Level Signal from Waste
Signal from 2 Position
Sensor Sensor Level Sensor
Sensors Interface Interface

Keypad
LCD Interface Serial Port
Interface

Pumps, Valves,
Motors and
Waste Level
Position Sensors Keypad LCD Module PC
Sensor

Hardware Boards 4-3

4.1.1 Main Control Unit
The main control unit uses a C8051 SCM and a CPLD to control and operate all
objects. The unit performs the following functions:

Communication with PC. The main control board communicates with PC

through RS232 port, which is for software upgrade.

User parameters storage. The board uses EEPROM to store parameters. The
operation is performed through SPI interface. 8k bytes or 100 user-defined
programs can be stored.

Motor control. The CPU controls motors directly.

Valve and pump control. The CPU controls pumps and valves directly.

Sensor signal detection. The CPLD detects signals from sensors and the
software reads sensor status. The sensors are the vertical home position sensor,
the horizontal home position sensor, the waste level sensor and the manifold
bump detection sensor.

LCD display control. The CPLD receives control and display data through the
extended CPU interface and then converts it into the data that conforms to the
LCD sequence. The CPLD also reads LCD status.

Keypad scanner. The 2*4 matrix keypad is controlled by the CPLD. When a key
is pressed, the CPLD detects it and produces a CPU interrupt to instruct the
CPU to read the key value. Two or more keys pressed simultaneously cannot
be supported.

Buzzer control. The CPLD converts instructions from CPU to signals that control
the buzzer.

4.1.2 Driver Unit

The driver unit drives:

Two step motors, which drives the manifold to move vertically and the plate
carrier to move horizontally. The motor driver uses two A3979 chips and adopts
16-subdivision drive to improve stability of motors and to decrease noise when
motors work at low speed. One chip drives one motor. The chip receives
direction, speed, current control and enable signals and outputs signals to drive
the motor.

Two pumps and two valves, which includes an air pump, a fluid pump, a
three-position valve and a two-position valve. The pump and valve driver uses
IRF3710 MOSFET to switch.

4.2 Keypad Board

The keys are on the keypad board and are installed into the system with the board.

4.3 Power Supply Board

The power supply board outputs:

+5V: 0.6A, which is supplied to the boards;

+12V: 4.0A (peak current), 2.0A (rated current)which is supplied to pumps and
valves;

+24V: 0.3A, which is supplied to step motors;

4-4 Hardware Boards


-12V: reserved.

The power supply board consists of:

EMI filter circuit;

Rectification and filter circuit;

Flyback converter, which outputs +12V, +24V and -12V;

Voltage reducing circuit, which generates +5V from the +24V input;

Overcurrent and overvoltage protection circuits for outputs.

4.4 LCD Module

The LCD module, which is a standard low-voltage graphic dot-matrix one, has a LCD
controller KS0108 and an integrated DDRAM. The module is operated by specific
control sequences and instructions.

4.5 Connecting Diagram

2 Position
LCD Module
Sensors

Vocuum
Pump

Fluid Pump APUMP J5 LCD J11 PHO J13 Bump Detection

J4
Sensor
J6 CRASH
Three-position
Valve
WPUMP
J19
VALVE1 Waste Level
J12
Two-position Sensor
J18 WASTE
Valve
VALVE2 Main Control Unit
Power J7 J1 Keypad Board
AC P1 Supply P2 J1 KEYBOARD
Board
POWER

J8 Serial Port
HMOTOR VMOTOR LED
J3 J2 J15 COM

Step Motor Step Motor

LED
(Horizontal) (Vertical)

Hardware Boards 4-5

4.6 Interface Definition
Three-
Two-position position
Power Supply
Valve Valve Fluid Pump
Board
- + - + - +

GND GND GND

VALVE2 VALVE1
WPUMP
J18 J19 J6
24V

12V
5V
PIN 1 PIN 1 PIN 1
PWR J1
PIN 1

NC

APUMP
B-

Vacuum
Pump
-
J5
J2
HMOTOR

PIN 1

+
B+

A-

A+ PIN 1

LCD_LED+
GND
LCD_RST
LCD_CS3
LCD_CS2
J11

LCD_CS1
LCD_EN
LCD_RW
B-
J3

LCD_RS
GND

LCD Module
VMOTOR

B+ GND
LCD

LCD_D7
Main Control Board

A- LCD_D6
LCD_D5
A+ PIN 1 LCD_D4
LCD_D3
LCD_D2
LCD_D1
GND
LCD_D0
LCD_VEE
LCD_VO
PIN 1 VCC +5V
GND

VPHO

Position
Vertical
Sensor
GND

+5V
PHO
J15

GND HPHO
J13

Horizontal
LED

Position
Sensor

LED+ PIN 1 GND

PIN 1 +5V
PIN 1

PIN 1

PIN 1

PIN 1

J8 J7 J12 J4

KEYBOARD
J14

WASTE CRASH
COM
NC
NC

Serial
Port
LEV1
LEVEL

GND

GND
K0 and K1 not connected

Bump
Waste Level
R0

C0

C2

C4

Detection
Sensor
Sensor

PC
R1

C1

C3

C5

Keypad Board

4-6 Hardware Boards

 R0
R1
C0
C1
C2
C3
C4
J1

Keypad Board

In the figure above, R0 means ROW0 (the row wire) and C0 means COL0 (the
column wire).

1
GND
VCC_+5V
LCD_VO
LCD_VEE
LCD_RS
LCD_RW
LCD_EN
LCD_D0
LCD_D1
LCD_D2
LCD_D3 LCD Module
LCD_D4
LCD_D5
LCD_d6
LCD_D7
LCD_RST
LCD_CS1
LCD_CS2
LCD_CS3
LCD_LED+
NC
21

Power Supply Board

P1 P2
PIN1

PIN1
NC

+12V

+24V
GND
N

-12V
L

GND

GND

GND

+5V

AC
Main Control Board
PWR J1

Hardware Boards 4-7

5 Maintenance and Service

5.1 Maintenance ......................................................................................................... 5-3

5.1.1 Daily Maintenance ................................................................................... 5-3
5.1.2 Weekly Maintenance ............................................................................... 5-3
5.1.3 Monthly Maintenance............................................................................... 5-3
5.2 Service.................................................................................................................. 5-4
5.2.1 Unclogging Needle .................................................................................. 5-4
5.2.2 Replacing Tubing for Three-position Valve.............................................. 5-4
5.2.3 Replacing Tubing for Two-position Valve................................................. 5-5
5.2.4 Troubleshooting ....................................................................................... 5-6
5.3 Software Upgrade............................................................................................... 5-10

Maintenance and Service 5-1

5-2 Maintenance and Service
 5 Maintenance and Service

5.1 Maintenance

5.1.1 Daily Maintenance

The following maintenance can be performed by users.

Clean the liquid splashed on the surface of the system.

Perform the tubing rinse procedure before shutting down the system. Perform
routine rinse for tubing with distilled water or deionized water.

5.1.2 Weekly Maintenance

The following maintenance can be performed by users.

Slightly pull the tubing connected to valves to move the tubing.

Use ethyl alcohol (50%) to clean the retaining screws on the manifold and the metal
electrodes on the manifold support.

5.1.3 Monthly Maintenance

The following maintenance can be performed by users.

Use ethyl alcohol (50%) to clean:

The manifold support.

The plate carrier and the spring sheet on the carrier.

The dispense and absorb needles.

Use warm water (about 40C) to wash the Wash1, Wash 2, Waste, Buffer and
distilled water bottles.

Maintenance and Service 5-3

5.2 Service

5.2.1 Unclogging Needle

During the cleaning, if a dispense needle injects little, or a absorb needle sucks little,
or the residue is much after the absorbing, the needle may be clogged. You can
unclog the needle as follows:

1 Find out which needle is clogged.

2 Slightly hold the manifold and make the needle face up. Use a stainless
needle whose diameter is about 0.5mm to unclog the needle.

3 After the unclogging is completed, reassemble the manifold onto the

system and run the rinse procedure to observe if the needle can work
properly.

5.2.2 Replacing Tubing for Three-position Valve

The tubing connected to the three-position valve may age or be damaged after being
used for a long time. You can replace it as follows:

1 Power off the system and disconnect the power cable.

2 Remove the screws on the rear panel and the bottom of the system. Open
the upper cover and place it on the right of the system.

5-4 Maintenance and Service

 3 Disconnect the tubing that connected to the three-position valve from the
Y-type connector and the Wash 1, Wash 2 connectors. Take out the tubing
connected to the outer channel of the valve first. Then slightly press the
black button on the end of the valve, and take out the tubing connected to
the inner channel of the valve and then release the button.

4 Cut two segments of new tubing. Ensure they are as long as the old ones.
Connect respectively the two segments of tubing to the Wash 1 and Wash
2 connectors on the inside of the rear panel. Then make them pass through
the holes in the rear panel. Press slightly the black button on the end of the
valve, connect the tubing connected to the Wash 2 connector to the inside
channel of the valve, and then release the button and connect the other
segment of tubing to the outer channel of the valve.
5 Put the two segments of tubing through their own installation holes on the
rear panel. Then connect them to the two mouths of the Y-type connector.
6 Reassemble the upper cover. Connect the power cable and turn on the
system. Put an used microplate on the plate carrier and then perform the
rinse procedure respectively with Wash 1 and Wash 2. Observe the
dispense needles to judge if the tubing can work properly.

5.2.3 Replacing Tubing for Two-position Valve

The tubing connected to the two-position valve may age or be damaged after being
used for a long time. You can replace it as follows:

1 Power off the system and disconnect the power cable.

2 Open the upper cover and place it on the right of the system.
3 Disconnect the tubing from the connectors. Use a hex wrench (diameter =
1.5mm) to slightly press the press head of the valve, and pull out the
tubing. Release the head.

Maintenance and Service 5-5

 4 Cut a segment of new tubing. Ensure it is 20mm longer than the old one.
Cut one end to the diagonal shape (about 20mm long) shown as the figure
below.

5 Connect the flat end of the new tubing to the bigger connector inside the
system. Pass the tubing through the corresponding installation hole in the
rear panel.
6 Use a hex wrench (diameter = 1.5mm) to slightly press the press head of
the valve and pull the diagonal end of the tubing through the hole in the
valve. After pulling the tubing in place, release the press head.

7 Cut the diagonal end (about 20mm long) of the tubing. Pull the tubing
through the installation hole in the rear panel and connect it to its
corresponding connector.
8 Reassemble the upper cover. Connect the power cable and turn on the
system. Run the tubing rinse procedure. Observe the dispense needles to
judge if the tubing can work properly.

BIOHAZARD
Wear gloves and lab coat and, if necessary, goggles.
In case your skin contacts the waste, follow standard laboratory safety
procedure and consult a doctor.

NOTE
When pulling the tubing through a hole, exercise caution to prevent it
from being damaged.

5.2.4 Troubleshooting

5.2.4.1 Manifold Failure

5-6 Maintenance and Service

 If the check mode is selected, when the connection of the manifold is contaminated
or the manifold is not in the right position, the following information will be displayed
during washing

Error!
Please check the manifold !
Press OK to continue or
EXIT to cancel !

You can correct the problem as follows:

1 Check if the manifold is placed correctly and not tilted, place it level if
needed. Press OK and check if the system works normally; or
2 Lift up the manifold, then wipe the retaining screws and metal plates with
clean cotton cloth dipped with ethanol. Install the manifold properly and
press OK; or

3 Restart the system and run the wash program.

4 If things like removing manifold during wash do not happen, in the
meantime standard micro plate is used with no conspicuous dimension
difference between the manifold and the micro plate, the default modeNo
check can be used.

5.2.4.2 Movement Error

During the startup or movements, if a movement error occurs, the system will warn
and display the following information:

Error!
XXXX movement error !
Restart or see the user
manual !

In the information above, XX represents horizontal or vertical.

You can correct the error as follows:

Maintenance and Service 5-7

 1 Switch off the power and restart the system; or
2 Switch off the power. In case of horizontal movement error, move the plate
carrier back and forth for 5 times. In case of vertical movement error, move
the manifold up and down for 5 times. Then restart the system and check if
it works normally.

5.2.4.3 Dispense Needle Error

During the wash, if a dispense needle injects little or no wash solution, you can do as
follows:

1 Check if the wash bottle is connected to the washer correctly. Reconnect

the tubing if needed; or
2 Check if the fluidic tubing is creased, stretch it if necessary; or
3 The fluidic tubing may adhere to each other if not moved for a long time. As
for tubing clamped by the three-position valve, first pull the outer tubing a
little, then keep pressing the black end of the three-position valve and pull
the inner tubing. As for tubing clamped by two-position valve, first run a
rinse procedure for about 20s, then pull the tubing quickly when the
dispense needle is dispensing liquid; or

4 Check if the tubing end inside the wash bottle is blocked. If it is, remove the
bottle cap and then unclog the tubing; or
5 Check if the manifold inlet and dispense needles are clogged. If they are,
remove the foreign matters from the manifold inlet using tweezers, and use
the supplied stainless needle to unclog the blocked dispense needles (see
the section 5.2.1 Unclogging Needle); or
6 Interchange the dispensing tubing and the waste tubing, then press MAINT
to backflush the dispensing tubing with air flow, drawing the foreign matters
into the waste bottle. Reconnect the tubing correctly and run a rinse
procedure again.

5.2.4.4 Residue Too Much

When excessive residue exists in microwell during washing process, you can correct
the error as follows:

1 Check if the waste/buffer bottle caps are tightened or sealed, or the fluidic
tubing is connected properly to the bottle cap. Tighten the bottle caps and
reconnect the fluidic tubing to the bottles; or

5-8 Maintenance and Service

 2 Check if the distance between aspirate needle and microwell bottom is too
large and not within 0.1-0.4mm. Youre recommended to set the bottom wash
height using the Bot Auto Ht function.
3 Check if the fluidic tubing is creased, stretch it if necessary; or
4 Check if the tubing end inside the waste or buffer bottle is blocked. If it is,
remove the bottle cap and then unclog the tubing; or
5 Check if the manifold inlet and aspirate needles are clogged. If they are,
remove the foreign matters from the manifold inlet using tweezers, and use
the supplied stainless needle to unclog the blocked aspirate needles; or
6 Interchange the aspiration tubing and the waste tubing, then press MAINT to
backflush the waste tubing with distilled water. Reconnect the tubing
correctly and run a rinse procedure again.
7 If the manifold has not been used for a long time, the connector might get
rusty, leading to the change or lifting of the connector. Follow the 5.1.2 to
clean the connector of the manifold. If the rust is very serious, use abrasive
paper of small granule to rub the rust away from the manifold retaining
screw.

5.2.4.5 Wash Solution Overflow

If the dispense needles dispense excessive wash solution into the microplate wells,
resulting in overflow, you can correct the error using the following methods:

1 Check if the waste/buffer bottle caps are tightened or sealed, or the fluidic
tubing is connected properly to the bottle cap. Tighten the bottle caps and
reconnect the fluidic tubing to the bottles; or
2 Check if the fluidic tubing is creased, stretch it if necessary; or
3 Check if the aspirate needles are far from the well. Check the dispensing
height and adjust the Disp Pos correctly; or
4 Check if foreign matters exist at two-position valve on the rear panel of the
washer, disabling the valve core to clamp the tubing tightly. Remove the
tubing clamped by the two-position valve and remove the foreign matters.

5.2.4.6 Collision of Manifold and Microplate

If the manifold collides with the microplate, causing the manifold to fall off or the
plate is tilted, you can handle the error using the following methods:

1 If the microplate is not placed properly on the carrier, reset the plate and
attach it to the carrier surface completely; or
2 Check the manifold position is set properly. Reset the crosswise aspiration
positions (Left Pos and Right Pos), ensuring the aspirate needles lie inside
the wells. Also check if the dispensing height is set correctly; or
3 Check if the plate carrier or manifold support is blocked. Remove the
foreign matters if any.

Maintenance and Service 5-9

 NOTE
The service life of consumables like plastic bottles, connectors or
manifold is about 24 months and that of tubing is about 18 months.

5.3 Software Upgrade

The system uses the serial port to communicate with a PC to upgrade and maintain
the software. The serial port locates on the rear panel of the system. The upgrading
procedure is as follows:

1 Use serial port cable to connect the PC and the MW-12A. Start the system and the
PC. Open the Download software P/N:110-000208-00on PC.

2 Select the parameter as the following figure.

Figure 5-1 Software upgrading

3 Select the file folder where the upgrading program is and open it, click Start.

4 After upgrading is completed, click Exit, disconnect the serial port cable.

5-10 Maintenance and Service

P/N: 0105-20-63177 (3.0)