Jurnal
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Jurnal
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ABSTRACT
Ruellia tuberosa L belongs to Acanthaceae family. In traditional medicine has been used as diuretic, antidiabetic, antipyretic and antihypertensive, and it also
recently been incorporated as a component in a herbal tea in Taiwan. This research aims to determine the antioxidant activity of R. tuberosa leaf extract.
Extraction of R. tuberosa leaf by stratified maceration method using dichloromethana and methanol. Methanol extract was partitioned by using ethyl acetate
and n-butanol. Antioxidant activity was tested by reduction of DPPH radical method and inhibition of the xanthine oxidase. The results show n-butanol
extract has an antioxidant activity with reduction of DPPH radical and inhibition of the enzyme xanthine oxidase with IC50 value respectively 7.42 and 0.15
g/mL.
Keywords: Acanthaceae, antioxidants, pletekan, Ruellia tuberosa Linn, xanthine oxidase.
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Table 1. Data of DPPH Radical Reduction
Sample Concentration Absorbance( 517 nm) % Inhibition IC50
(g/mL) Blank Sample (g/mL)
Dichlorometana 5 0,8170,0017 3,02
10 0,8150,0020 3,26 14,57
20 0,843 0,8120,0018 3,62
30 0,8040,0032 4,57
40 0,8020,0009 4,77
50 0,7820,0009 7,19
Regression equation y = 2,289 + 3,274x
5 0,8020,0024 4,83
10 0,7990,0005 5,23
Ethyl acetate 20 0,843 0,7870,0020 6,63 8,79
30 0,7690,0011 8,68
40 0,7620,0007 9,61
50 0,7560,0011 10,26
Regression equation y = 4,173 + 5,211x
n-butanol 5 0,843 0,8060,0003 4,34
10 0,8050,0014 4,46 7,42
20 0,7750,0008 8,04
30 0,7720,0006 8,42
40 0,7610,0007 9,65
50 0,7470,0016 11,31
Regression equation y = 3,673+ 6,242x
Water 5 0,720 0,7160,0009 0,54 21,69
10 0,7110,0001 1,26
20 0,7060,0021 1,86
30 0,7050,0011 1,99
40 0,7020,0012 2,43
50 0,6890,0001 4,22
Regression equation y = 0,712 + 2,272x
Methanol 5 0,7000,0006 2,75 11,55
10 0,6930,0001 3,79
20 0,6920,0002 3,92
30 0.720 0,6800,0005 5,57
40 0,6780,0012 5,87
50 0,6630,0013 7,87
Regression equation y = 2,289 4,129x
Quercetin 5 0.653 0,6470,0007 0,92 2,87
10 0,6260,0003 4,13
15 0,6180,0011 5,36
20 0,6020,0014 7,81
25 0,5870,0001 10,11
Linear equation y = -0,952 + 17,684x
BHT 5 0,903 0,8690,0005 3,69 3.17
10 0,8390,0002 7,02
15 0,8220,0002 8,90
20 0,8080,0002 10,43
25 0,7970,0002 11,74
Linear equation y = 2,501 + 15,624x
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Figure 1. R. tuberosa L.
The research results showed antioxidant activity with IC50 free electron of DPPH radical has been paired with electrons
values of each extract the dichloromethane extract 14.57, from traps compounds (antioxidants) would reduce of DPPH
methanol 11.55, ethyl acetate 8.79, n-butanol 7.42 and water radical (DPPH-H), and form stable compounds are DPP-
21.69 g/mL. The antioxidant activity with IC50<10 g/mL Hydrazine12.
value is the smallest (good antioxidant) are n-butanol and Inhibition of xantin oxidase enzyme (XOD) assay on all
ethyl acetate extract and included in the category of extract of R. tuberosa leaf and allopurinol as a standard.
extremely powerful antioxidants. Extract of dichloromethane, Activity of the XOD is indicated by the formation of uric
methanol and water show IC50 values were in the range 10-50 acid15 with measured using UV-Vis spectrophotometry at a
g/mL included in powerful antioxidants class13. The process wavelength of 295 nm in aerobic conditions16. In this study,
of reduction of free radicals through the mechanism of testing was conducted at the optimum conditions, so that will
donation hydrogen from antioxidant compounds. Free be the determination of the optimum conditions and the
radicals used are synthetic DPPH reacts with an antioxidant determination of the maximum wavelength. The optimum
compound through the donation of electrons from an condition at temperature of 30oC, pH 7.8 and 0.15 mM of
antioxidant compound to get a pair of electrons. DPPH substrate concentration and maximum wavelength at 284 nm.
radical compound deep purple would fade to yellow if it is Inhibition of xanthine oxidase. Allopurinol is used as
reduced by antioxidants into non radical DPPH14, when the standard with IC50 value 0.02 g/mL. Extract of R. tuberosa
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Aktsar Roskiana Ahmad et al. IRJP 2012, 3 (11)
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