Guide To Protein Purification, Volume 463

View on ScienceDirect
Guide to Protein Purification, Volume 463

Institutional Access
Tax Exempt Orders

Support Center
Table of Contents
1. Why Purify Enzymes?
Murray Deutscher and Arthur Kornberg
Section I: Developing Purification Procedures
2. Strategies and Considerations for Protein Purifications
Stuart Linn
3. Use of Bioinformatics in Planning a Protein Purification
Richard R. Burgess
4. Preparing A Purification Summary Table

Richard R. Burgess
Section II: General Methods for Handling Proteins and Enzymes
5. Setting Up A Laboratory
Murray P. Deutscher
6. Buffers: Principle and Practice
Vincent S. Stoll and John S. Blanchard
7. Measurement of Enzyme Activity
T. K. Harris and M. M. Keshwani
8. Quantitation of Protein
James E. Noble and Marc J. A. Bailey
9. Concentration of Proteins and Removal of Solutes
Jonathan K. Romero, David R. H. Evans and Matthew Westoby
10. Maintaining Protein Stability
Murray P. Deutscher
Section III: Recombinant Protein Expression and Purification
11. Selecting an Appropriate Method for Expressing a Recombinant Protein
William H. Brondyk
12. Bacterial Systems for Production of Heterologous Proteins
Frank R. Collart, Ashley M. Frank and Sarah Zerbs
13. Expression in the Yeast Pichia Pastoris
James M. Cregg, Ilya Tolstorukov, Anasua Kusari, Jay Sunga, Knut Madden and Thomas
Chappell
14. Baculovirus-Insect Cell Expression Systems
Donald L. Jarvis
15. Recombinant Protein Production By Transient Gene Transfer Into Mammalian Cells
Sabine Geisse and Cornelia Fux
16. Tagging For Protein Expression
Arun Malhotra
17. Refolding of Solubilized Inclusion Body Proteins
Richard R. Burgess
Section IV: Preparation of Extracts and Subcellular Fractionation
18. Advances In Preparation of Biological Extracts for Protein Purification
Anthony C. Grabski
19. Isolation of Subcellular Organelles and Structures
Jrg Von Hagen and Uwe Michelsen
Section V: Purification Procedures: Bulk Methods
20. Protein Precipitation Techniques
Richard R. Burgess
21. Affi-Gel Blue for Nucleic Acid Removal and Early Enrichment of Nucleotide Binding
Proteins
Murray P. Deutscher
Section VI: Purification Procedures: Chromatographic Methods
22. Ion Exchange Chromatography
Alois Jungbauer and Rainer Hahn
23. Gel Filtration
Earle Stellwagen
24. Protein Chromatography on Hydroxyapatite Columns
Larry J. Cummings, Mark A. Snyder and Kimberly Brisack

25. Theory and Use of Hydrophobic Interaction Chromatography in Protein Purification
Applications
Justin T. Mccue
Section VII: Purification Procedures: Affininty Methods
26. Affinity Chromatography: General Methods
Marjeta Urh, Dan Simpson and Kate Zhao
27. Immobilized-Metal Affinity Chromatography (IMAC)
Frank Schfer* Helena Block, Barbara Maertens, Anne Spriestersbach, Nicole Brinker,
Jan Kubicek, Roland Fabis And Jrg Labahn
28. Identification, Production, and Use of Polyol-Responsive Monoclonal Antibodies for

Immunoaffinity Chromatography
Nancy E. Thompson, Katherine M. Foley, Elizabeth S. Stalder, And Richard R. Burgess
Section VIII: Purification Procedures: Electrophoretic Methods
29. One-Dimensional Gel Electrophoresis
David E. Garfin
30. Protein Analysis Using High-Resolution Two-Dimensional Polyacrylamide Gel

Electrophoresis and Isoelectric Focusing
Friedman
31. Protein Gel Staining Methods: An Introduction And Overview
Thomas H. Steinberg
32. Elution of Proteins From Gels
Richard R. Burgess
33. Immunodetection By Protein Blotting Performing and Optimizing Western Blots With
An Emphasis on Chemiluminescent Detection
Alice Alegria-Schaffer, Krishna Vattem and Andrew Lodge
Section IX: Purification Procedures: Membrane Proteins And Glycoproteins

34. Detergents: An Overview
Dirk Linke
35. Purification of Membrane Proteins
Sue-Hwa Lin and Guido Guidotti
36. Purification of Recombinant G-Protein-Coupled Receptors
Reinhard Grisshammer
37. Cell-Free Translation Of Integral Membrane Proteins Into Unilamelar Liposomes
Michael A. Goren, Akira Nozawa, Shin-Ichi Makino, Russell L. Wrobel and Brian G.
Fox
Section X: Characterization of Purified Proteins
38. Determination of Protein Purity
David G. Rhodes and Thomas M. Laue
39. Determination of Size, Molecular Weight, and Presence of Subunits
David G. Rhodes, Robert E. Bossio and Thomas M. Laue
40. Identification and Quantification of Protein Posttranslational Modifications
Andrew Link
41. Parallel Methods For Expression And Purification
Scott A. Lesley
42. Techniques To Isolate O2-Sensitive Proteins: [4Fe-4S]-FNR As An Example
Aixin Yan and Patricia J. Kiley
43. Rethinking Your Purification Procedure
Murray Deutscher
44. Important But Little Known (Or Forgotten) Artifacts in Protein Biochemistry
Richard R. Burgess
Description
The 2e of this classic Guide to Protein Purification provides a complete update to existing
methods in the field, reflecting the enormous advances made in the last two decades. In
particular, proteomics, mass spectrometry, and DNA technology have revolutionized the field
since the first editions publication but through all of the advancements, the purification of
proteins is still an indispensable first step in understanding their function. This volume examines
the most reliable, robust methods for researchers in biochemistry, molecular and cell biology,
genetics, pharmacology and biotechnology and sets a standard for best practices in the field. It
relates how these traditional and new cutting-edge methods connect to the explosive
advancements in the field. This "Guide to" gives imminently practical advice to avoid costly
mistakes in choosing a method and brings in perspective from the premier researchers while
presents a comprehensive overview of the field today.
Key Features
Gathers top global authors from industry, medicine, and research fields across a wide
variety of disciplines, including biochemistry, genetics, oncology, pharmacology,
dermatology and immunology
Assembles chapters on both common and less common relevant techniques
Provides robust methods as well as an analysis of the advancements in the field that, for
an individual investigator, can be a demanding and time-consuming process

Guide To Protein Purification, Volume 463

Uploaded by

Copyright:

Available Formats

Guide To Protein Purification, Volume 463

Uploaded by

Document Information

Original Description:

Original Title

Copyright

Available Formats

Share this document

Share or Embed Document

Sharing Options

Did you find this document useful?

Is this content inappropriate?

Copyright:

Available Formats

Guide To Protein Purification, Volume 463

Uploaded by

Copyright:

Available Formats

View on ScienceDirect

Guide to Protein Purification, Volume 463

Tax Exempt Orders

Murray Deutscher and Arthur Kornberg

Section I: Developing Purification Procedures

2. Strategies and Considerations for Protein Purifications

3. Use of Bioinformatics in Planning a Protein Purification

4. Preparing A Purification Summary Table

Section II: General Methods for Handling Proteins and Enzymes

6. Buffers: Principle and Practice

Vincent S. Stoll and John S. Blanchard

7. Measurement of Enzyme Activity

T. K. Harris and M. M. Keshwani

James E. Noble and Marc J. A. Bailey

9. Concentration of Proteins and Removal of Solutes

Jonathan K. Romero, David R. H. Evans and Matthew Westoby

10. Maintaining Protein Stability

Section III: Recombinant Protein Expression and Purification

11. Selecting an Appropriate Method for Expressing a Recombinant Protein

12. Bacterial Systems for Production of Heterologous Proteins

Frank R. Collart, Ashley M. Frank and Sarah Zerbs

13. Expression in the Yeast Pichia Pastoris

14. Baculovirus-Insect Cell Expression Systems

Sabine Geisse and Cornelia Fux

16. Tagging For Protein Expression

17. Refolding of Solubilized Inclusion Body Proteins

Section IV: Preparation of Extracts and Subcellular Fractionation

18. Advances In Preparation of Biological Extracts for Protein Purification

19. Isolation of Subcellular Organelles and Structures

Jrg Von Hagen and Uwe Michelsen

Section V: Purification Procedures: Bulk Methods

20. Protein Precipitation Techniques

Section VI: Purification Procedures: Chromatographic Methods

22. Ion Exchange Chromatography

Alois Jungbauer and Rainer Hahn

23. Gel Filtration

24. Protein Chromatography on Hydroxyapatite Columns

Larry J. Cummings, Mark A. Snyder and Kimberly Brisack

Section VII: Purification Procedures: Affininty Methods

26. Affinity Chromatography: General Methods

Marjeta Urh, Dan Simpson and Kate Zhao

27. Immobilized-Metal Affinity Chromatography (IMAC)

28. Identification, Production, and Use of Polyol-Responsive Monoclonal Antibodies for

Nancy E. Thompson, Katherine M. Foley, Elizabeth S. Stalder, And Richard R. Burgess

Section VIII: Purification Procedures: Electrophoretic Methods

29. One-Dimensional Gel Electrophoresis

30. Protein Analysis Using High-Resolution Two-Dimensional Polyacrylamide Gel

31. Protein Gel Staining Methods: An Introduction And Overview

32. Elution of Proteins From Gels

Alice Alegria-Schaffer, Krishna Vattem and Andrew Lodge

Section IX: Purification Procedures: Membrane Proteins And Glycoproteins

35. Purification of Membrane Proteins

Sue-Hwa Lin and Guido Guidotti

36. Purification of Recombinant G-Protein-Coupled Receptors

37. Cell-Free Translation Of Integral Membrane Proteins Into Unilamelar Liposomes

Section X: Characterization of Purified Proteins

38. Determination of Protein Purity

David G. Rhodes and Thomas M. Laue