BSC Biotechnology Syllabus
BSC Biotechnology Syllabus
BSC Biotechnology Syllabus
UNIVERSITY OF MUMBAI
Programme : B.Sc.
Course: Biotechnology
2016 2017
Preamble:
The need of the hour is to design appropriate syllabi which keeps pace with
changing times and technology with emphasizes on applications while
elucidating technology in depth. The present Syllabi is Restructured anticipating
the future needs of Biotechnology Sector with more emphasis on imparting
hands-on skills. The main thrust is laid on making syllabus compatible with
developments in Education, Research and Industrial sectors. The Theory and
Practical course in new restructured course will lead to impart skill-set
essentials to further Biotechnology Sector.
USBT103 Core Subject Basic Life Sciences-I : Biodiversity and Cell Biology 2 3
Ability
USBT107 Societal Awareness 2 3
Enhancement
Course 1 (FC I)
USBTP101,
USBTP102, Core Subject Practicals of USBT101, USBT102, USBT103, 6 18
USBTP103 Practicals USBT104, USBT105 and USBT106
Semester II
Course Code Course Type Course Title Credits Lectures/Week
Basic Chemistry-I
Nomenclature andClassification of
Organic Compounds: Alkanes,Alkenes,
Alkynes, Cyclic Hydrocarbons, Aromatic
Compounds, Alcohols and Ethers,
Aldehydes and Ketones, Carboxylic Acids
and its derivatives, Amines, Amides, Alkyl
Halides and Heterocylic Compounds
Chemical Bonds: 15 Lectures 30 hrs
Unit II Ionic Bond: Nature of Ionic Bond, Structur
Chemical Bonds eof NaCl, KCl and CsCl, factors influencing
the formation of Ionic Bond.
SEMESTER I
Basic Chemistry-II
Gravimetric Analysis:
Solubility and Precipitation, Factors
affecting Solubility, Nucleation, Particle
Size, Crystal Growth, Colloidal State,
Ageing/Digestion of Precipitate.
Co-Precipitation and Post-Precipitation.
Washing, Drying and Ignition of Precipitate.
(Numericals Expected).
Methods of Seperation 15 Lectures 30 hrs
Unit III Precipitation, Filtration, Distillation and
Analytical Techniques Solvent Extraction.
Analytical Techniques
Chromatography:
Definition, Principles, Types
Introduction to Paper Chromatography,
Thin Layer Chromatography, Column
Chromatography and its
Applications.Colorimetry:
Principle, Beer-Lamberts Law,
Measurement of Extinction, Derivation of
E = kcl, Limitations of Beer-Lambarts
Law, Filter Selection
SEMESTER I
Introduction to Animal
Diversity:Non-Chordates and
Chordates {with at least one
representative example.)
SEMESTER - I
SEMESTER I
SEMESTER II
Chemistry-II : Physical Chemistry
COURSE CODE TITLE CREDITS Notional
Hours
USBT 202 Physical Chemistry 2
Course Objectives : To aquaint students with concepts in Thermodynamics, Kinetics and Redox
Reactions
Learning Outcome : To impart skills in Kinetics and Chemical Reactions
Thermodynamics: 15 lectures 30 hrs
Unit I System, Surrounding, Boundaries
Thermodynamics Sign Conventions, State Functions,
Internal Energy and Enthalpy:
Significance, examples, (Numericals
expected.)
Laws of Thermodynamics and its
Limitations, Mathematical expression.
Qualitative discussion of Carnot Cycle
for ideal Gas and Mechanical
Efficiency. Laws of Thermodynamics
as applied to Biochemical Systems.
Concept of Entropy, Entropy for
Isobaric, Isochoric and Isothermal
Processes.
Reaction Kinetics: 15 lectures 30 hrs
Unit II Rate of Reaction, Rate Constant,
Chemical Kinetics Measurement of Reaction Rates Order
& Molecularity of Reaction,
Integrated Rate Equation of First and
Second order reactions (with equal
initial concentration of reactants).
(Numericals expected)
Determination of Order of Reaction
by a) Integration Method b) Graphical
Method c) Ostwalds Isolation Method
d) Half Time Method. (Numericals
expected).
Principals of Oxidation & Reductio 15 lectures 30 hrs
Unit III n Reactions
Oxidation Reduction Oxidising and Reducing Agents, Oxid
reactions ation Number,
Rules to assign Oxidation Numbers wi
th examples Ions like
Oxalate, Permanganate and Dichromat
e. Balancing Redox Reactions by Ion
Electron Method
Oxidation, Reduction, Addition and
Substitution & Elimination Reactions.
SEMESTER II
Life Sciences-I : Physiology and Ecology
COURSE CODE TITLE CREDITS Notional
Hours
USBT 203 Physiology and Ecology 2
Course Objectives: To aquaint studetns with Physiological Processes in Plants and Animals
Learning Objectives : To impart the knowledge of Physiology and Ecology
Photosynthesis, Intracellular 15 lectures 30 hrs
Unit I Organization of Photosynthetic
Plant Physiology System. Fundamental Reactions of
Photosynthesis, Photosynthetic
Pigments, Role of Light. Hill Reaction
and its Significance, Light Reactions,
Cyclic and Non-Cyclic Photo induced
Electron Flow, Energetics of
Photosynthesis, Photorespiration,
Dark Phase of Photosynthesis, Calvin
Cycle, C-3, C-4 pathways
Physiology of Respiration,
Mechanism of Respiration
Principles of Gaseous Exchange in the
Blood and Body Fluids
SEMESTER II
Life Sciences-II : Genetics
COURSE CODE TITLE CREDITS Notional
Hours
USBT 204 Genetics 2
Course Objectives : To aquaint students with concepts in Genetics
Learning Objectives : To impart skills in Techniques in Genetic Analysis and Population Genetics
Mendels Laws of Heredity 15 lectures 30 hrs
Unit I Monohybrid Cross: Principle
Genetics Fundamentals of Dominance and Segregation.
Dihybrid Cross: Principle of
Independent Assortment.
Application of Mendels
Principles
Punnett Square.
Mendels Principle in Human
Genetics.
Incomplete Dominance and
Co-dominance.
Multiple Alleles.Allelic series.
Variations among the effect of the
Mutation.
Genotype andPhenotype.
Environmental effect on the
expression of the Human Genes.
Gene Interaction.
Epistasis.
Genetic analysis in Bacteria- Prototrop 15 lectures 30 hrs
Unit II hs, Auxotrophs.
Microbial Genetics Bacteriophages: Lytic and Lysogenic
Development of Phage.
Mechanism of Genetic Exchange in
Bacteria:
Conjugation; Transformation; Transd
uction; (Generalized Transduction,
Specialized Transduction)
Bacterial Transposable Elements.
Genetic Structure of Populations 15 lectures 30 hrs
Unit III Genotypic Frequencies and Allelic
Population Genetics Frequencies,
Hardy- Weinberg Law and its
assumptions
Genetic Variations in Populations-
Measuring Genetic Variation at Protei
n Level and measuring Genetic
Variations at DNA level
Natural Selection.
Genetic Drift
Speciation
Role of Population Genetics in
Consevation Biology
SEMESTER II
Biotechnology-I : Tissue Culture &Scientific Writing and Communication Skills
SEMESTER - II
Biotechnology-II : Enzymology, Immunology and Biostatistics
COURSE CODE TITLE CREDI Notional
TS Hours
USBT 206 Enzymology, Immunology and Biostatics 2
Course Objectives : To aquaint students with concepts in Enzymology, Immunology and
Biostatistics
Learning Outcome : To impart the skills in Enzyme Kinetics, Immunological Techniques and
Biostatistics
Definition, Classification, Nomenclatu 15 30 hrs
Unit I re, Chemical Nature, Properties of lectures
Enzymes Enzymes,
Mechanism of Enzyme Action,
Active Sites, Enzyme Specificity,
Effect of pH, Temperature, Substrate
Concentration on Enzyme Activity,
Enzyme Kinetics, Michelis-Menten
Equation,
Types of Enzyme Inhibitions-Competi
tive, Uncompetitive, Non-Competitive
Allosteric Modulators Co-Factors,Zym
ogens,
Overview of Immune Systems, Cell and 15 30 hrs
Unit II Organs involved, T and B cells. lectures
Immunology Innate Immunity, Acquired Immunity,
Local and Herd Immunity, Humoral
and Cellular Immunity - Factors
Influencing and Mechanisms of each.
Antigens and Antibodies: Types of
Antigens, General Properties of
Antigens, Haptens and Superantigens
Discovery and Structure of Antibodies
(Framework region) Classes of
Immunoglobulins, Antigenic
Determinants.
Antigen-Antibody Interactions
Monoclonal Antibodies,
Vaccines (Live, Killed) and Toxoid.
Problems with Traditional Vaccines,
Impact of Biotechnology on Vaccine
Development.
Defination &Importance of Statistics i 15 30 hrs
Unit III n Biology lectures
Biostatistics Types of Data, Normal and Frequency
Distribution
Representation of Data and Graphs
(Bar Diagrams, Pie Charts and
Histogram, Polygon and Curve)
Types of Population Sampling
Measures of Central Tendency
(For Raw,Ungroup & Group Data)
Mean
Median
Mode
Measures of Dispersion
Range, Variance, Coefficient of
Variance.
Standard Derivation.
Standard Error.
Semester II
PRACTICALS
SEMESTER II
Practicals
Chemistry
COURSE CODE TITLE CREDITS Notional
Hours
USBTP 201 Chemistry 2 30 hrs
1. Spot test for Carbohydrates, Fats and Proteins and Amino Acids and Nucleic Acids
2. Standardization of Colorimeter and Estimation of Reducing sugar by DNSA method
3. Estimation of Protein by Biuret method and Lowry method
4. Saponification of Fats, Saponification Value of Oil or Fat, Iodine value of Oiland determine the
rate constant for the saponification reaction between ethyl acetate and NaOH by back titration
method
5. To determine enthalpy of dissolution of salt like KNO3
6. Determine the rate constant for hydrolysis of ester using HCl as a catalyst
7. Study the kinetics of reaction between Thiosulphate ion and HCl
8. Study reaction between potassium Persulphate and Potassium Iodide kinetically and hence to
determine order of reaction
9. Study the reaction between NaHSO3 and KMnO4 and balancing the reaction in acidic, alkaline
and neutral medium
10. Study transfer of electrons (Titration of sodium thiosulphate with potassium dichromate)
11. Determination of the volume strength of hydrogen peroxide solution by titration with
standardised potassium permagnate solution
12. Determination of amount of K oxalate and oxalic acid in the given solution Titrimetrcially
SEMESTER II
Practicals
Life Sciences
COURSE CODE TITLE CREDITS Notional
Hours
USBTP 202 Life Sciences 2 30 hrs
1. Study of Hills reaction
2. Colorimetric study of Absorption Spectrum of Photosynthetic Pigments
3. Movemnt of Food in Paramoecium
4. Activity of Salivary Amylase on Starch
5. Analysis of Urine
6. Study of Mammalian Blood, Blood count using Haemocytometer and estimation of
Haemoglobin in Mammalian Blood
7. Study of Human Blood Groups
8. Study of Mammalian Kidney and Heart
9. Problems in Mandelian Genetics
10. Study of Mitosis and Meiosis
11. Study of Karyotypes Normal Male and Normal Female
12. Study of Interactions Commensalism, Mutualism, Predation and Antibiosis, Parasitism.
SEMESTER II
Practicals
Biotechnology
COURSE CODE TITLE CREDITS Notional
Hours
USBTP 203 Biotechnology 2 30 hrs
1. Working and use of various Instruments used in Biotechnology Laboratory (Autoclave, Hot air Oven,
Centrifuge, Incubator, Rotary Shaker, Filter Assembly, LAF, pH meter and Colorimeter)
2. Laboratory Organization and Layout for Plant and Animal Tissue Culture Laboratory
3. Preparation of Stock Solutions and Preparation of Media for PTC
4. Aseptic Transfer Technique, Surface Sterilization and Innoculation for Callus Culture
5. Media Preparation and Sterilization (ATC)
6. Trypsinization of Tissue and Viability Count
7. Qualitative Assay of Enzyme Amylase. Lipase, Protease, Urease, Catalase and Dehydrogenease
8. Enzyme Kinetics : Study of the effect of pH, Temperature on activity of Enzyme
9. Study of Effect of Substrate Concentration on enzyme activity and determination of Vmax and
Km
10. Study of antigen antibody interaction by Ouchterlony method
11. Biometric Analysis for Mean, Median, Mode and Standard Deviation and Data representation
using frequency Polygon, Histogram and Pie Diagram
12. Preparation of review reports of 5 Scientific Papers and Presentation (last 5 years)
Semester I and II
Ability Enhancement Course 1 (FC I)
Ability Enhancement Course 2 (FC II)
SEMESTER I
Ability Enhancement Course 1 (FC I)
Societal Awareness
COURSE CODE TITLE CREDITS Notional
Hours
USBT 107 Societal Awareness 2
Course Objective : To aquaint the students with concepts of Societal Awarness
Learning Outcome : To impart knowledge of Society and make students aware about the Problems in Society
Understand the multi-cultural diversity of Indian 15 Lectures 30 hrs
Unit I society through its demographic composition:
population distribution according to religion,
Overview of Indian Society caste, and gender; Appreciate the concept of
linguistic diversity in relation to the Indian
situation; Understand regional variations
according to rural, urban and tribal
characteristics; Understanding the concept of
diversity as difference
Concept of Disparity- I 15 Lectures 30 hrs
Unit II Understand the concept of disparity as arising out
of stratification and inequality;
Concept of Disparity Explore the disparities arising out of gender with
special reference to violence against women,
female foeticide (declining sex ratio), and
portrayal of women in media;Appreciate the
inequalities faced by people with disabilities and
understand the issues of people with physical and
mental disabilities
Concept of Disparity-II
Examine inequalities manifested due to the caste
system and inter-group conflicts arising thereof;
Understand inter-group conflicts arising out of
communalism; Examine the causes and effects of
conflicts arising out of regionalism and linguistic
differences
The Indian Constitution 15 lectures 30 hrs
Unit III Philosophy of the Constitution as set out in the
The Indian Constitution and Preamble; The structure of the Constitution-the
Preamble, Main Body and Schedules;
Significant Aspects of Fundamental Duties of the Indian Citizen;
Political Processes tolerance, peace and communal harmony as
crucial values in strengthening the social fabric of
Indian society; Basic features of the Constitution
Significant Aspects of Political Processes
The party system in Indian politics; Local self-
government in urban and rural areas; the 73rd
and 74th Amendments and their implications for
inclusive politics; Role and significance of women
in politics
Topics for Project Guidance: Growing Social Problems in India:
Substance abuse impact on youth & challenges for the future
HIV/AIDS awareness, prevention, treatment and services
Problems of the elderly causes, implications and response
Issue of child labour magnitude, causes, effects and response
Child abuse effects and ways to prevent
Trafficking of women causes, effects and response
SEMESTER II
Ability Enhancement Course 2 (FC II)
Globalization, Ecology and Sustainable Development
COURSE CODE TITLE CREDITS Notional
Hours
USBT 207 Globalization, Ecology and Sustainable 2
Development
Course Objective : To aquaint the students with concepts of Globalization, Ecology and Environment
Learning Outcome : To impart knowledge of Globalization make students aware about the Problems in Society
Globalisation and Indian Society Understanding the 15 Lectures 30 hrs
Unit I concepts of liberalization, privatization and
Globalisation and Indian globalization;Growth of information technology and
communication and its impact manifested in
Society and
everyday life; Impact of globalization on industry:
Human Rights
changes in employment and increasing migration;
Changes in agrarian sector due to globalization; rise
in corporate farming and increase in farmers
suicides.
Human Rights
Concept of Human Rights; origin and evolution of
the concept; The Universal Declaration of Human
Rights;Human Rights constituents with special
reference to Fundamental Rights stated in the
Constitution
Ecologyand Sustainbale Development 15 Lectures 30 hrs
Unit II Importance of Environment Studies in the current
developmental context; Understanding concepts of
Ecology
Environment, Ecology and their interconnectedness;
and Environment as natural capital and connection to
Sustainbale Development quality of human life; Environmental Degradation
causes and impact on human life; Sustainable
development, concept and components; poverty and
environment
Understanding Stress and Conflict 15 lectures 30 hrs
Unit III Causes of stress and conflict in individuals and
Understanding and Managing society; Agents of socialization and the role played
by them in developing the individual; Significance of
Stress and Conflict in
values, ethics and prejudices in developing the
Contemporary Society
individual; Stereotyping and prejudice as significant
factors in causing conflicts in society. Aggression and
violence as the public expression of conflict
Managing Stress and Conflict in Society
Types of conflicts and use of coping mechanisms for
managing individual stress; Maslows theory of
selfactualisation;Different methods of responding
to conflicts in society; Conflictresolution and efforts
towards building peace and harmony in society
Topics for Project Guidance: Growing Social Problems in India:
Increasing urbanization, problems of housing, health and sanitation;
Changing lifestyles and impact on culture.
Farmers suicides and agrarian distress.
Debate regarding Genetically Modified Crops.
Development projects and Human Rights violations.
Increasing crime/suicides among youth.
Evaluation Scheme
The performance of the learners shall be evaluated into TWO Parts.
The learners performance shall be assessed by Internal Assessment with 25 marks & by conducting the
Semester End Examinations with 75 marks .
Practical Training will have Practical Examination for 50 marks at the end of Semester.
The allocation of marks for the Internal Assessment and Semester End Examinations are as follows:-
I. Internal Exam-25 Marks
(i) Test 20 Marks
(ii) Activities - 5 Marks
II. External Examination- 75 Marks
(i) Theory Question Paper Pattern:-
All questions areCompulsory.
Question Based on Marks
Q.1 Unit I 20
Q.2 Unit II 20
Q.3 Unit III 20
Q.4 Unit I,II and III 15
All questions shall be compulsory with internal choice within the questions.
Each Question may be sub-divided into sub questions as a, b, c, d &e, etc & the
allocation of Marks depends on the weightage of the topic.
III. Practical Examination 300 marks (50 marks x 6 core papers)
Each Core Subject Carries 50 Marks
Chemistry : 30 marks + 10 marks (Journal)+ 10 marks(Viva-voce)
Life Sciences and Biotechnology : Major (20 marks), Minor (10 marks), Identification /Spots (10
marks), Viva-voce (5 marks), Journal (5 marks)
IV. Ability Enhancement Course
V. Internal Exam-25 Marks
(iii)Project 20 Marks
(iv) Activities - 5 Marks
VI. External Examination- 75 Marks