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    5090 s03 Ir 3

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    Ahmed Kaleem Khan Niazi
    Instructions for preparing apparatus give details of the apparatus required by each candidate for each experiment in this paper. A summary of the questions that will be presented to the candidates is included. If a candidate breaks any of the apparatus, or loses any of the material supplied, the matter should be rectified. Supervisors are advised to remind candidates that all substances in the examination should be treated with caution.

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    5090 s03 Ir 3

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    Ahmed Kaleem Khan Niazi
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    Instructions for preparing apparatus give details of the apparatus required by each candidate for each experiment in this paper. A summary of the questions that will be presented to the candidates is included. If a candidate breaks any of the apparatus, or loses any of the material supplied, the matter should be rectified. Supervisors are advised to remind candidates that all substances in the examination should be treated with caution.

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    Instructions for preparing apparatus give details of the apparatus required by each candidate for each experiment in this paper. A summary of the questions that will be presented to the candidates is included. If a candidate breaks any of the apparatus, or loses any of the material supplied, the matter should be rectified. Supervisors are advised to remind candidates that all substances in the examination should be treated with caution.

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    5090 s03 Ir 3

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    Ahmed Kaleem Khan Niazi
    Instructions for preparing apparatus give details of the apparatus required by each candidate for each experiment in this paper. A summary of the questions that will be presented to the candidates is included. If a candidate breaks any of the apparatus, or loses any of the material supplied, the matter should be rectified. Supervisors are advised to remind candidates that all substances in the examination should be treated with caution.

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    CAMBRIDGE INTERNATIONAL EXAMINATIONS

    General Certificate of Education Ordinary Level


    BIOLOGY 5090/03
    Paper 3 Practical Test
    May/June 2003

    1 hour 15 minutes
    CONFIDENTIAL INSTRUCTIONS

    Great care should be taken that any confidential information given does not reach
    the candidates either directly or indirectly.

    Instructions for preparing apparatus

    These instructions give details of the apparatus required by each candidate for each experiment in this
    paper. A summary of the questions that will be presented to the candidates is included, where appropriate,
    to allow the Biology teacher to test the apparatus appropriately. No access to the question paper is
    permitted in advance of the examination session.

    If a candidate breaks any of the apparatus, or loses any of the material supplied, the matter should be
    rectified and a note made in the Supervisor’s Report.

    If arrangements are made for different sessions for different groups of candidates, care must be taken to
    ensure that the different groups of candidates are effectively isolated so that no information passes
    between them.

    Supervisors are advised to remind candidates that all substances in the examination should be treated with
    caution. Pipette fillers and safety goggles should be used where necessary.

    In accordance with the COSHH (Control of Substances Hazardous to Health) Regulations, operative in the
    UK, a hazard appraisal of the examination has been carried out.

    The following codes are used where relevant.

    C = corrosive substance F = highly flammable substance


    H = harmful of irritating substance O = oxidising substance
    T = toxic substance

    If you have any problems or queries regarding these Instructions, please contact CIE
    by e-mail: [email protected],
    by phone: +44 1223 553554,
    by fax: +44 1223 553558,
    stating the Centre number, the nature of the query and the syllabus number quoted above.

    This document consists of 3 printed pages and 1 blank page.


    SP (SLC/KN) S41692/2
    © CIE 2003 [Turn over
    2

    Each candidate must be provided with the following apparatus and materials.

    To be supplied by the Centre

    Question 1

    (i) Three large test-tubes, 2.5 cm in diameter, labelled T1, T2 and T3, set up as shown in the
    diagram.

    T1 to contain 2 cm depth of hydrogencarbonate indicator solution that has been acidified by


    the addition of 1 drop of 1M hydrochloric acid. The solution should turn yellow. A fresh leaf
    should be suspended above the solution by trapping the petiole with a rubber bung. The test-
    tube should then be covered with aluminium foil and labelled T1.
    T2 to contain 2 cm depth of hydrogencarbonate indicator solution that has been made
    [C] alkaline by the addition of 1 drop of 1M sodium hydroxide. The solution should turn
    red/purple. A fresh leaf should be suspended above the solution by trapping the petiole with a
    rubber bung. The test-tube should be labelled T2.
    The leaves in tubes T1 and T2 should be of the same species and similar size, shape and
    colour.
    T3 to contain 2 cm depth of hydrogencarbonate indicator solution. The solution should be
    orange/red.

    Hydrogencarbonate indicator solution may be purchased ready-made or made up as follows.


    • Dissolve 0.2 g thymol blue and 0.1 g cresol red powders in 20 cm3 of ethanol.
    • Dissolve 0.84 g of sodium hydrogencarbonate in 900 cm3 of deionised water.
    • Add the alcoholic solution to the hydrogencarbonate solution and make up the volume to
    1 litre with deionised water.
    • Shortly before use, dilute the appropriate amount of this solution 10 times i.e. add nine
    times its volume of deionised water.

    Note Before use, the solution must be brought into equilibrium by bubbling atmospheric air
    through the solution until it has turned orange/red. This should be done using a filter pump or
    an aquarium pump. This may take several hours.

    (ii) A large test-tube, 2.5 cm in diameter, with a rack or means of support.

    (iii) A drinking straw.

    5090/03/INST/M/J/03
    3

    (iv) Two leaves, labelled L1 and L2.


    L1 should be taken from a healthy, well watered plant that has been kept in complete
    darkness for at least 5 days to ensure complete de-starching. The leaf should then be
    [F] immediately prepared for a starch test by being placed in boiling water and then in hot alcohol
    to decolourise it. Once white, the leaf should be labelled L1.
    L2 should be taken from a healthy, well watered plant, that has been kept in bright light for at
    least 12 hours in order to produce starch. The leaf should then be immediately prepared for a
    [F] starch test by being placed in boiling water and then in hot alcohol to decolourise it. Once
    white, the leaf should be labelled L2.

    [F] Note These leaves can be prepared in advance and stored in 70% alcohol.
    You are advised to carry out sample starch tests with some of the leaves well in advance of
    the examination session.

    (v) Iodine solution with dropper, labelled as such.

    (vi) A white tile.

    Question 2

    (i) Fruit S1 which should be a winged fruit from a tree such as Acer (maple), Fraxinus (ash),
    Ailanthus (tree of heaven) or Tipuana (tipu). Please ensure that the winged fruit used is
    identified on the back page of the first candidate’s script.

    (ii) A hand lens.

    (iii) A ruler.

    (iv) A microscope slide with cover slip and a mounted needle.

    (v) A grape, not seedless variety, labelled S2. If grapes cannot be obtained, substitute a similar
    fruit, with sweet flesh enclosing one or a few seeds.

    (vi) Benedict’s solution with dropper, labelled as such.

    (vii) A test-tube with a rack or a means of support.

    (viii) A water-bath for Benedict’s test.

    (ix) Access to water.

    (x) A paper towel.

    The Supervisor’s Report is to be filled in on the back page of the first candidate’s script. Please
    ensure that this is done.

    5090/03/INST/M/J/03
    4

    BLANK PAGE

    5090/03/INST/M/J/03

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