T I B C PDF
T I B C PDF
T I B C PDF
www.biolabo.fr
Total Iron Binding Capacity
MANUFACTURER:
BIOLABO SA, Treatment of specimens for the determination of Total Iron Binding Capacity
02160, Maizy, France of transferrin in human serum and plasma.
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TECHNICAL SUPPORT AND ORDERS
Tel : (33) 03 23 25 15 50
IVD IN VITRO DIAGNOSTIC USE
Fax : (33) 03 23 256 256
Magnesium carbonate
MATERIAL REQUIRED BUT NOT PROVIDED
With measuring spoon
1. Basic medical analysis laboratory equipment.
1. Normal and pathological control sera.
3. Reagent for Iron determination REF 92108 or REF 80008.
SAFETY CAUTIONS
BIOLABO reagents are designated for professional, in vitro diagnostic CALIBRATION
use.
Refer to technical data sheet of the reagent used for Iron determination.
• Verify the integrity of the contents before use.
• Use adequate protections (overall, gloves, glasses).
• Do not pipette by mouth. QUALITY CONTROL
• In case of contact with skin or eyes, thoroughly wash affected areas • BIOLABO EXATROL-N (Level I) REF 95010.
with plenty of water and seek medical advice. • BIOLABO EXATROL-P (Level II) REF 95011.
• Material Safety Data Sheet is available upon request. • Assayed control sera referring to the same method.
• Waste disposal : Respect legislation in force in the country. • External quality control program.
All specimens should be handled as potentially infectious, in accordance
with good laboratory practices using appropriate precautions. Respect Refer to technical data sheet of the reagent used for Iron determination.
legislation in force in the country.
Version : AT 92308 23 01 2009
EXPECTED VALUES (1)(2) MANUAL PROCEDURE
Let stand reagents and specimens at room temperature.
Specimen (*) 1 mL
0-4 days 186-393 [33.3-70.4]
Iron Solution (vial R1) 2 mL
3 months-16 years 290-515 [52.0-92.3]
Mix, wait 10 minutes then add :
16-60 years, male 307-522 [55.0-93.5]
Precipitant (vial R2) Using the spoon, measure a
16-60 years, femal 358-543 [64.1-97.3] dose (approx 150 mg), level off
and transfer the contents into
60-90 years 272-536 [48.7-96.1] the tube
> 90 years 266-496 [47.7-88.9] Mix well by inversion. Allow to stand at room temperature for 30 minutes,
shaking on a rotary or swinging agitator. Centrifuge 10 minutes at 3000 RPM.
(*) 1 mg transferrin binds 1.43 6g of iron according to following formula : Measure Iron in supernatant, with reagent REF 80008 or REF 92108.
Serum transferrin (g/L) = 0.007 T.I.B.C. (6g/dL)
Each laboratory should establish its own normal ranges for the Note : Iron is stable for 1 hour in supernatant.
population that it serves. (*) See § LINEARITY.
REFERENCES
(1) TIETZ N.W. Text book of clinical chemistry, 3rd Ed. C.A. Burtis, E.R. Ashwood,
W.B. Saunders (1999) p. 1699-1703.
(2) Clinical Guide to Laboratory Test, 3rd Ed., N.W. TIETZ (1995) p. 604-605 and
p.376.
(3) YOUNG D.S., Effect of Drugs on Clinical laboratory Tests
th
4 Ed. (1995) p.3-364
(4) Ramsay W. N. M., in « Advances in Clinical Chemistry », H. Sobotka et C. P.
Steward Ed., Academic Press (1958), vol.1, p.1
Made in France
Manufacturer Use by IVD In vitro diagnostic Temperature limitation REF Catalogue number See insert LOT Batch number