25 Years of Mushroom AICRIP
25 Years of Mushroom AICRIP
25 Years of Mushroom AICRIP
(Mushroom)
O.P. Ahlawat
Satish Kumar
T. Arumuganathan
R.P. Tewari
Published by:
Director
National Research Centre for Mushroom (ICAR)
Chambaghat, Solan – 173 213 (HP), INDIA
Phone: 01792-230451; Fax: 01792-231207
E-mail: [email protected]; [email protected]
Website: www. nrcmushroom.org
© N.R.C.M. 2008
All rights reserved. No part of this publication may be reproduced in any
form or by any means without prior permission in writing from the
competent authority.
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CONTENTS
Page No.
Foreward v
Abbrevations xiii
3. Genetic Resources 17
5. Technologies Generated 35
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FOREWARD
Mushroom cultivation is a potential biotechnological process wherein
the waste plant materials or negative value crop residues can be converted
into valuable food. Protein conversion efficiency and productivity of
mushrooms per unit land area and time is far superior than plant and animal
sources. In recent years mushroom cultivation in India has witnessed a
tremendous growth with respect to the type of mushrooms and their
producitivity. The mushroom market is growing continously mainly due to
increasing interest in their culinary, nutritional and health benefits. On
recognizing the importance of mushroom as an eco-friendly alternative for
agro-waste recycling, capability to provide better nutrition for the vast
vegetarian population, employment generation and a good income source,
organized research on mushroom was initiated in India with the
establishment of National Research Centre for Mushroom and All India
Coordinated Research Project on Mushroom in 1983 at Solan (HP).
During the past 25 years of its implementation, the project had been
instrumental in bring out superior strains of white button mushroom, paddy
straw mushroom, milky mushroom, oyster mushroom, superior casing
materials for button mushroom, post harvest technology and standardization
of cultivation technology of specialty mushrooms which are needed to
diversify the mushroom cultivation scenario in the country.
Optimizing the yield of oyster, button, milky, paddy straw and shiitake
mushrooms by way of using different substrates, chemicals, casing materials
and supplements, identifying safer chemicals for improving shelf-life of
button mushroom, packaging and drying of mushrooms, isolation and
identification of thermophilic fungi, germplasm collection and conservation,
and survey and surveillance for diseases and insect-pests of mushrooms
will further improve the production and productivity of mushrooms and
will foster socio-economic upliftment of the mushroom growers throughout
the country. The extension services along with supply of quality spawn has
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EXECUTIVE SUMMARY
1. All India Coordinated Research Project on Mushroom (AICRPM) was
initiated during VIth Five-Year Plan on 01.04.1983 with its
Headquarters at National Research Centre for Mushroom, Solan (HP).
4. The financial outlay of the project since its inception in VIth plan till
Xth plan had been Rs. 838.13 Lacs.
5. The activities of the AICRP Centres are monitored through the visits
of Project Coordinator, monitoring teams, quarterly and annual
reports, regular group meetings / workshops and high powered
Quinquennial Review Teams (QRTs) constituted by the Indian Council
of Agricultural Research. Since the inception of the project, 10
workshops/ group meetings have been held in such a manner that
venues are evenly distributed through out the country. During these
meetings recommendations for release of varieties, cultivation
technologies, and crop protection measures are brought out. Based on
the previous results and keeping in view the emerging problems, new
technical programmes are also worked out in these meetings.
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17. In casing materials evaluation, 2 years old spent compost, FYM + spent
compost (1:1, v/v) and FYM+ soil+ sand+ spent compost (1:1:1:1, v/
v) out yielded the other treatments.
18. The hot water treatment of the substrate resulted into significantly
higher yield of oyster mushroom (720 g/kg dry substrate) than other
two methods of pasteurization (chemical and solar pasteurization) of
substrate.
22. Dipping of oyster mushroom substrate in the solutions of 0.4 and 0.6
% of effective microorganisms preparations was found to significantly
increase the mushroom yield by 50 %.
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28. Silver oak saw dust+wheat bran (8 %) was found to be the best
substrate for the cultivation of Auricularia polytricha with an average
yield of 811.7 g/ kg of substrate.
29. Supplementation of wheat straw with wheat bran and rice bran was
found to enhance the yield of black ear mushroom.
32. The acceptable white colour of button mushroom was retained upto
48 h on packaging mushrooms in 100 gauge PP bags followed by
storage at both refrigerated and ambient conditions.
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35. Blanching followed by sun drying was found best method for drying of
Pleurotus sajor-caju in comparison to cabinet drying-unblanched and
blanched methods.
36. Wheat straw compost was found better than paddy straw compost for
growing Agaricus bisporus. Initial level of 1.5 % N in the compost
proved to be optimum for getting higher yield of A. bisporus strain, S-
11.
38. Most of the pathogens and pests of white button mushroom can be
managed by sound hygiene and sanitation measures which are the best
available options with a grower at the moment. The use of chemicals
namely Bavistin and Sporgone should be applied only under extremely
unavoidable circumstances.
39. Survey and surveillance of diseases and insect-pests are carried out at
regular intervals and the growers are advised for various components
of IPM. They are (i) Farm hygiene, (ii) Providing doors, windows and
ventilators with 35 mesh nylon net to prevent entry of the flies in the
cropping room, (iii) Use of light and yellow sticky traps for monitoring
and killing mushroom flies, (iv) Cooking out at 70- 800C at least for 2
hours before removing the beds to kill all stages of pests and diseases,
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40. All the Centres carried out the extension activities by participating in
State/National level Exhibitions, Melas, Kisan Gosthies, mass
communication through AIR & TV programmes, replying letters,
telephones, e-mails and on the spot guidance during farm visits. Almost
all the Centres conducted training programmes of different durations
for farmers, farmwomen, unemployed youths and the entrepreneurs
of their areas.
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ABBREVIATIONS
AICRPM - All India Coordinated Research Project on Mushroom
HP - Himachal Pradesh
NRCM - National Research Centre for Mushroom
UP - Uttar Pradesh
ICAR - Indian Council of Agricultural Research
UGC - University Grants Commission
DSR - Days taken for Spawn Run
sq. ft. - Square Feet
Rs. - Rupees
TNAU - Tamil Nadu Agricultural University
Govt. - Government
R&D - Research and Development
MT - Metric Tons
% - per cent
m. ha. - Million Hectare
C.G. - Chhattisgarh
kg - kilo gram
spp. - species
viz. - namely
IGAU - Indira Gandhi Agricultural University
g - gram
i.e. - that is
FYM - Farm Yard Manure
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h - hour
@ - at the rate
EDTA - Ethylene di-amine tetra acetic acid
ppm - parts per million
KMS - potassium meta bisulphate
PP - polypropylene
PE - polyethylene
K2HPO4 - Di-potassium Hydrogen Phosphate
LMC - Long Method Compost
DDVP - Dichlorvos (Nuvon)
°C - degree Celsius
BE - Biological Efficiency
DCSR - Days for Spawn Run
MEA - Malt Extract Agar
pH - Hydrogen ion concentration
cm - centimeter
q - quintal
“ - inch
etc. - etcetera
Fig. - Figure
N - Nitrogen
m2 - Square meter
DAS - Days After Sowing
P - phosphorous
K - potassium
C/N ratio - Carbon to Nitrogen ratio
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1. ALL INDIA COORDINATED RESEARCH
PROJECT ON MUSHROOM : AT A GLANCE
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I. CROP IMPROVEMENT
Experiment Location
Imp. 1: Wild germplasm collection, Pune, Pantnagar, Solan, Udaipur, Ludhiana,
identification and conservation Faizabad, Coimbatore, Raipur, Vellayani,
Ranchi, Barapani
Imp. 2: Testing of different strains of Pune, Pantnagar, Solan (Pasteurized
white button mushroom, compost), Udaipur, Ludhiana, Faizabad
Agaricus bisporus (Long method compost)
Imp. 3: Strain evaluation of milky Pune, Pantnagar, Solan, Udaipur, Ludhiana,
mushroom, Calocybe indica Faizabad, Coimbatore, Raipur, Vellayani,
Ranchi, Barapani
Imp.4: Strain evaluation of paddy Ludhiana, Faizabad, Coimbatore, Raipur,
straw mushroom, Volvariella volvacea Vellayani, Ranchi, Barapani
Imp. 5: Strain evaluation of oyster Faizabad, Udaipur, Raipur, Vellayani,
mushroom (Pleurotus sajor-caju and Ranchi, Barapani
Pleurotus florida)
Imp. 6: Evaluation of the yield potential Faizabad, Ludhiana, Udaipur, Raipur,
of Pleurotus fossulatus Pantnagar, Ranchi, Barapani
Experiment Location
1. Button mushroom
Prod. 7: Isolation and identification of Pune, Pantnagar, Solan, Udaipur,
thermophilic microorganisms from white Ludhiana, Faizabad
button mushroom compost from different
locations for their exploitation in rapid
composting process
Prod. 8: To test different locally available Pune, Solan, Udaipur, Ludhiana
agro-industrial waste for their feasibility
as casing material
2. Oyster mushroom
Prod. 9: Effect of chemical sprays on Pune, Pantnagar, Solan, Udaipur,
yield of Pleurotus spp. Ludhiana, Faizabad, Coimbatore, Raipur,
Vellayani, Ranchi, Barapani
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Experiment Location
Prod. 10: Evaluation of the yield potential Pune, Pantnagar, Solan, Udaipur,
of blue oyster mushroom (Hypsizygus Ludhiana, Faizabad, Coimbatore, Raipur,
ulmarius) on different substrates Vellayani, Ranchi, Barapani
3. Paddy straw mushroom
Prod. 11: Cultivation of paddy straw Ludhiana, Faizabad, Coimbatore, Raipur,
mushroom, Volvariella volvacea Vellayani, Solan, Barapani
4. Milky Mushroom
Prod. 12: Cultivation of milky mushroom, Ludhiana, Faizabad, Pune, Raipur,
Calocybe indica Udaipur, Pantnagar, Vellayani, Ranchi
5. Shiitake Mushroom
Prod. 13: Cultivation of shiitake Udaipur, Pantnagar, Coimbatore, Raipur,
mushroom, Lentinula edodes Pune, Ludhiana, Solan
Experiment Location
Pht. 14: Washing treatment for button Pune, Pantnagar, Solan, Udaipur,
mushroom, Agaricus bisporus Ludhiana, Faizabad
Pht. 15: Packaging for button mushroom, Pune, Pantnagar, Solan, Udaipur,
A.bisporus Ludhiana, Faizabad
Pht. 16: Drying of oyster mushroom, Ludhiana, Udaipur, Coimbatore, Raipur,
Pleurotus spp. Vellayani, Ranchi
Pht. 17: Drying of milky mushroom, Pune, Pantnagar, Udaipur, Ludhiana,
Calocybe indica Faizabad, Coimbatore, Raipur, Vellayani,
Ranchi, Barapani
Experiment Location
Prot. 18: Survey and Surveillance Pune, Pantnagar, Udaipur, Ludhiana,
of diseases and insect-pests in mushroom Solan, Faizabad, Coimbatore, Raipur,
farms in different regions Vellayani, Ranchi, Barapani
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2. HISTORY OF AICRPM CENTRES
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3. GENETIC RESOURCES
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4. REGION SPECIFIC MUSHROOM VARIETIES
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In Uttarakhand, three
mushroom species are cultivated
round the year by the growers . They
are as under:
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5. TECHNOLOGIES GENERATED
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veils did not open upto second day Blanching of oyster mushroom
of storage. (Pleurotus florida) for 2 min in
0.2 % salt+0.1 % citric acid
The whiteness of button solution before drying and
mushroom could be increased storage (for 3 months) slightly
with dipping treatment in 100 improved the whiteness. Direct
ppm, 150 ppm, 200 ppm and 500 sun drying or cabinet drying with
ppm EDTA and 100 ppm EDTA out chemical treatment or
+ 0.02% KMS over unwashed blanching kept superior
mushroom. At ambient brittleness after 3 months of
temperature storage in Udaipur, storage.
whiteness was less as compared
to storage at 5°C in 150, 200, 500 Sun drying method was found
ppm EDTA and 100 ppm EDTA best for drying of Pleurotus sajor-
+ 0.02 % KMS washed caju in comparison to cabinet
mushrooms. drying, unblanched and
blanched methods (Fig. 29).
Button mushroom fruiting
bodies could be well preserved up
to 3 days under ambient
temperature at Raipur and 11-16
days under refrigerated
conditions after giving a dipping
treatment in 200 ppm and 500
ppm solutions of EDTA and KMS,
respectively.
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6. CROPPING SYSTEM RESEARCH
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Table 10: Mushrooms suitable for different zones of Uttarakhand state as per
climatic conditions
Zone Region District Mushroom species &
(height from Number of crops
sea level)
Zone-‘A1’ Tarai, U.S. Nagar, Haridwar, Button - 2 crops (October-March)
(100-600 m) Bhawar Nainital, Pauri Garhwal, Oyster - 2+2 crops (August-October
Champawat, Dehradun & February-April)
Milky - 2 crops (April-September)
Shiitake - 1 crop (November-
February)
Black ear - 2 crops (July-October)
Zone-‘A2’ Lower hill Champawat, Nainital, Button - 2 crops (October-April)
(600-1000 m) Pauri Garhwal, Oyster - 2 crops (April-November)
Dehradun, Almora Milky - 1 crop (May-July)
Shiitake - 1 crop (November-
February)
Black ear - 2 crops (April-October)
Zone-‘B’ Medium Champawat, Nainital, Button - 2 crops (October-March)
(1000-1500 hill Almora, Dehradun, Tehri Oyster - 2 crops (August-March)
m) Garhwal, Bageshwar, Milky - 2 crops (April-September)
Pauri Garhwal, Chamoli, Shiitake - 1 crop (November-
Pithoragarh February)
Black ear - 2 crops (July-October)
Zone-‘C’ High hill Pithoragarh, Chamoli, Button - 3 crops (February-
(1500-2400 Bageshwer, Uttar Kashi, November)
m) Dehradun, Tehri Garhwal, Oyster - 2 crops (May-August)
Rudra Prayag Shiitake - 2 crops (March-October)
Zone-‘D’ Higher Pithoragarh, Chamoli, Button - 3 crops (February-October)
(>2400 m) hill Uttarkashi Oyster - 2 crops (June-July)
Shiitake - 2 crops (March-October)
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7. STRATEGIES FOR PEST AND
DISEASE MANAGEMENT
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8. TECHNOLOGIES TRANSFERRED AND
EXTENSION SERVICES RENDERED
A complete package of
technologies has been transferred
for the growers of the state and
adjoining areas through University
‘Package of Practices’.
Technologies transferred
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Academics
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Table 13: M.Sc and Ph.D thesis work conducted at Pune Centre
Sr. Title Name of Name of Year Degree
No. student guide
1 Studies on efficacy of wheat D. Venkata Dr. D.M. 2000 M.Sc.
straw and sugarcane trash on Reddy Sawant (Ag.)
yield and nutrient status of
A.bitorquis
2 Studies on competitive moulds V.D. Surya- Dr. D.M. 2000 M.Sc.
in straw substrate for Pleurotus wanshi Sawant (Ag.)
sajor-caju and their management
3 Effects of supplementation of S.S. Dangat Dr. P.V. Wani 2002 M.Sc.
wheat straw on performance of (Ag.)
Pleurotus eous (Berk.) Sacc.
4 Effect of different casing Ms. V.M. Prof. S.K. 2002 M.Sc.
materials on the yield of button Jadhav Jadhav (Ag.)
mushroom (A. bisporus)
5 Applied aspects of cultivation of C.T. Kumbhar Dr. P.V. Wani 2003 Ph.D.
Pleurotus eous (Berk) Sacc. (Ag.)
6 Studies on competitive molds in K.V. Harphale Dr. P.V. Wani 2003 M.Sc.
Pleurotus eous (Berk) Sacc. (Ag.)
7 Effect of size and shape of bed P.S. Ilhe Dr. S.W. 2003 M.Sc.
on growth and yield of Pleurotus Jadhav (Ag.)
sajor-caju
8 Hybridization studies in S.G. Sawashe Dr. D.M. 2003 Ph.D.
Pleurotus spp. Sawant (Ag.)
9 Hybridization studies in S.W. Jadhav Dr.D.M. 2003 Ph.D
Agaricus bisporus Sawant (Ag.)
10 Effect of biofertilizers on yield A.B. Gaikwad Dr. S.S. 2004 M.Sc.
of mushroom Pleurotus sajor- Wange (Ag.)
caju
11 Effect of microbial inoculants Ms. A.S. Dr. S.S. 2005 M.Sc.
on white button mushroom Gitay Wange (Ag.)
(Agricus bisporus)
12 Studies on Hypsizygus R.N. Patil Dr. S.S. 2006 M.Sc.
ulmarius- A new edible Wange (Ag.)
mushroom
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Fig. 36. Ministers from Sri Lanka and Gujarat visiting AICRPM Centre, Coimbatore
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Haridwar Marginal 03 09 11 31
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and the technology has been more than 3000 growers from
transferred to farmers of the State. different parts of State. TV
Regular training programmes on programmes were recorded and
cultivation of different varieties of telecasted on production of oyster
mushrooms were conducted for and other important mushrooms.
unemployed youth, members of Regular advisory services were
kudumbasree units, VHSE students, extended from the Centre to the
senior citizens and members of mushroom growers of the state. Self-
residents association (Fig. 43). help programmes were also
Advanced training on production of conducted with technical expertise
milky mushroom was imparted to of the staff of the university.
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9. BENEFIT REAPED BY THE FARMERS
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Few cold storage chambers have been more than 160 mushroom farms in
converted to suit the mushroom the State with a production capacity
cultivation in some of these districts. of 2665 MT/year. There are 5 units
engaged in production of value-
9.2. Maharashtra added mushroom products viz.,
mushroom soup powder, mushroom
Commercial production of button biscuits, mushroom pickles,
mushroom in Maharashtra was mushroom papad and mushroom
started in 1972. Initially it was done powder capsule.
in potato cold storage with some
alterations in the structural designs. 9.3. Tamil Nadu
The total mushroom production in
the Maharashtra was 300 MT during Among different kinds of
1992-93 and it has increased to 2500 mushrooms, the farmers of Tamil
MT in 2005-06. More than hundred Nadu prefer to grow oyster and milky
and fifty growers are engaged in the mushrooms because of their simple
oyster mushroom cultivation, cultivation techniques. Total
marketing, spawn production and production of mushrooms in the
processing. The popularity of milky state is about 18,000 MT/ annum. In
mushroom is also increasing among oyster mushroom, Pleurotus florida,
the growers and the demand for P. eous, and Hypsizygus ulmarius are
milky mushroom is more in commercially grown in all parts of
Mumbai, Pune and coastal Konkan Tamil Nadu. About 400 spawn
region. Few growers have production units and more than 680
successfully started growing milky mushroom growers are distributed
mushroom in the state. through out the State.
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10. MILESTONES AND LAMP POSTS
OF THE PROJECT
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11. INFORMATION GATEWAYS
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12. ATTRIBUTES OF MUSHROOM VARIETIES/STRAINS/
TECHNOLOGIES RELEASED
Important attributes of recommended mushroom strains and technologies released by
AICRP (mushroom)
Variety / Strain / Compost / casing requirements Salient features and response to biotic
Technology & abiotic stresses
NCS-100 of Agaricus Pasteurized compost High yielding single spore isolate
bisporus Peak yield in the first flush
Fruiting bodies tough with short stipe, average
weight 7.2
NCS-101 of A.bisporus Pasteurized/unpasteurized Small stipe; pileus stipe ratio 2.28 as compared
compost to 2.17 in strain S-11
Medium size fruiting bodies; average fruiting
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body weight 8.2g as compared to 9.5g in strain
S-11
Peak yield in second flush
Suitable for cultivation in plains in winter under
natural /partially controlled conditions
Fruiting bodies are tough with good post harvest
quality
NCH-102 of A.bisporus Pasteurized compost High yielding hybrid variety
Suitable for cultivation on compost prepared by
short method especially suitable for the hilly
regions of the country
Fruiting bodies tough with short stipe
P-1 of A.bisporus Pasteurized/unpasteurized Long stipe, fruiting bodies open quickly, dark
compost brown gills
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Variety / Strain / Compost / casing requirements Salient features and response to biotic
Technology & abiotic stresses
MS – 39 of A. bisporus Pasteurized/unpasteurized Medium sized fruiting bodies, light brown gills,
compost average toughness
NCB – 6 of A. bitorquis Pasteurized compost Grows at a temperature of 22-26oC
High yielding strain
Mushroom with superior shelf–life
Produces large size excellent quality smooth
fruiting bodies
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NCB – 13 of A. bitorquis Pasteurized compost Grows at a temperature of 22-26oC
Prolific yielding strain
Produces large size excellent quality smooth
fruiting bodies suitable for fresh market
Fruiting bodies with superior shelf life
Fruiting bodies about 3-5.5cm in dia
Resistant to known mushroom viruses
Yields 15 kg mushrooms from 100 kg compost
in 6 weeks of cropping
Pleurotus florida Chemical treated or steam Suitable for cultivation under winter conditions
pasteurized substrate between a temperature range of 10 – 20oC
Fruiting bodies of light brown to pallid yellow
in colour and 6 – 12 cm in size, grows in clusters
Gives very high mushroom yield under
favourable temperature conditions
Variety / Strain / Compost / casing requirements Salient features and response to biotic
Technology & abiotic stresses
Pleurotus sajor-caju Chemical treated or steam Suitable for warmer regions of the country and
pasteurized substrate requires 22 – 28oC temperature for optimum
mushroom yield
Popular for its attractive shape, size and flavour
Fruiting bodies with 4-12 cm dia pileus and 2 –
5 cm long stalk and greyish brown in colour,
grows single or in clusters
Chemical treatment of Chemical treatment with 25 ppm Assured crop of oyster mushroom
substrate for oyster concentration of carbendazim and No infection from weed fungi like Trichoderma,
mushroom cultivation 500 ppm of formalin for 12 – 14 Penicillium, Sclerotium etc.
hours
Cultivation technology Overnight soaking of saw dust, Suitable under tropical and sub-tropical climates
of Black ear mushroom Draining of excess water, Popular for stomach and gastric disorders
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(Auricularia polytricha) Mixing of wheat or rice bran 15-20 days for spawn run
@5.0% on dry weight basis, Fruiting starts after 20-25 days of spawning
Filling in 2 – 3 kg capacity in
polypropylene bags of 30 x 20 cm size,
plugging followed by autoclaving at
22 p.s.i. for 2 hours,
Mixing of wheat grain or saw dust
based spawn @ 5.0% and incubation
for 15 – 20 days for spawn run at
22-280C, Making of longitudinal slits of
10 cm x 2 cm size and hanging of
the bags in cropping rooms,
Maintaining of 80 – 85 % RH, temperature
25-320C, fresh air (2 – 3 times) each for 10 min/
day and 4 – 5 hours of diffused light
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Variety / Strain / Compost / casing requirements Salient features and response to biotic
Technology & abiotic stresses
Supplementation of Neem cake @ 4 % on dry weight Higher mushroom yield and better quality
oyster mushroom basis during summer conditions or fruiting bodies
substrate with nitrogen wheat or rice bran @ 4 % on dry Strict watch on bed temperature is needed as
rich supplements weight basis during winter conditions supplements tend to increase the temperature
by 6 – 7oC
Spray treatment of 0.1% Spraying of beds at the time of pin Higher mushroom yield by 20 – 35%
rooting hormone head initiation of 1st, 2nd and 3rd Higher mushroom yield during first 15 days of
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in compost at the time bacteria @ 0.5 – 1.0% in ready to mushrooms
of spawning spawn compost at the time of spawn Lower incidence of competitor moulds in
mixing. compost
Heavier and whiter fruiting bodies
Early first harvest by 2 – 3 days (post-spawning)
Early spawn run by 2-3 days and no effect on
case run
1.5% initial Nitrogen Nitrogen level between 1.5 to 1.75% Congenial for button mushroom growth
level in compost at the beginning of composting and Minimum growth of competitor moulds
C:N ratio in 25-30:1 Higher mushroom yield
Wheat straw/paddy Can be used alone and in combination Cheap and easily available
straw as the substrate with each other Give good growth of button mushroom
for button mushroom Higher mushroom yield
compost preparation
Variety / Strain / Compost / casing requirements Salient features and response to biotic
Technology & abiotic stresses
Shortening of the Maintaining of initial Nitrogen level Saving of time and labour
composting period for of 1.5% in compost and turning Higher compost from unit weight of the basal
button mushroom schedule at 0,2,4,6,8,10,12,14,16 ingredients
cultivation days and spawning
CACing: Spawned The contaminants free spawn run Reduction in time taken for case run
95
casing or mixing of compost is mixed in casing material Controlled and synchronized flushes
spawn run compost in at the time of casing Enhanced mushroom yield
casing material
Chemical sterilization Mixing of Formalin and Bavistin Checking of competitor moulds and yellow mould
of long method compost @ 1.5 liter and 50 gm/ton compost, disease
respectively 48 hours before Higher mushroom yield
spawning.
25 Years of AICRP (Mushroom)