Scribd Logo
Upload

Welcome to Scribd!

  • 64 views

    Biology Amylase Experiment

    Uploaded by

    Red Velvet
    The document describes two experiments investigating how temperature and pH affect the activity of enzymes. The first experiment measures how amylase activity changes with temperature by testing how quickly starch is broken down into maltose. Results show optimal activity around body temperature. The second examines amylase activity under neutral, acidic and alkaline conditions, finding it works best in alkaline pH. A third experiment is outlined to similarly test the effect of pH on catalase's ability to break down hydrogen peroxide.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd

    Biology Amylase Experiment

    Uploaded by

    Red Velvet
    64 views3 pages
    The document describes two experiments investigating how temperature and pH affect the activity of enzymes. The first experiment measures how amylase activity changes with temperature by testing how quickly starch is broken down into maltose. Results show optimal activity around body temperature. The second examines amylase activity under neutral, acidic and alkaline conditions, finding it works best in alkaline pH. A third experiment is outlined to similarly test the effect of pH on catalase's ability to break down hydrogen peroxide.

    Original Description:

    IGCSE Biology

    Copyright

    © © All Rights Reserved

    Available Formats

    DOCX, PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    The document describes two experiments investigating how temperature and pH affect the activity of enzymes. The first experiment measures how amylase activity changes with temperature by testing how quickly starch is broken down into maltose. Results show optimal activity around body temperature. The second examines amylase activity under neutral, acidic and alkaline conditions, finding it works best in alkaline pH. A third experiment is outlined to similarly test the effect of pH on catalase's ability to break down hydrogen peroxide.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    64 views3 pages

    Biology Amylase Experiment

    Uploaded by

    Red Velvet
    The document describes two experiments investigating how temperature and pH affect the activity of enzymes. The first experiment measures how amylase activity changes with temperature by testing how quickly starch is broken down into maltose. Results show optimal activity around body temperature. The second examines amylase activity under neutral, acidic and alkaline conditions, finding it works best in alkaline pH. A third experiment is outlined to similarly test the effect of pH on catalase's ability to break down hydrogen peroxide.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    of 3

    Chapter 1: Life Processes─Enzymes

    Investigating the Effect of Temperature on the Activity of Amylase


    ˙ Amylase is an enzyme found in saliva and in the alimentary canal. It can digest starch into maltose.
    ˙ In this investigation, you will measure the action of amylase by measuring the rate at which starch is
    disappearing.
    ˙ Iodine test will be used to test for the presence of starch.
    Iodine Test Colour

    ˙
    ˙ The experimental optimum temperature (40°C) is very close to the optimum temperature in scientific
    literature for amylase is (37°C).
    What variables could have been better controlled?
    ˙ The temperatures tested were not consistent. Mixed amylase and starch solution should be kept inside of water
    bath at all times.
    ˙ Multiple trials to increase accuracy. Each temperature needs to be tested multiple times.
    ˙ Independent Variable (x-axis) - the variable that is changed in an experiment to test the effects on the
    dependent variable
    Temperature
    ˙ Dependent Variable (y-axis) - the variable being tested and measured in an experiment
    The Activity of Amylase

    1
    Chapter 1: Life Processes─Enzymes

    Effect of pH on the Action of Amylase


    Procedure:
    1. Soak 10 beans in water for two days.
    2. Remove the seed coat. Grind them with 2 cm3 of distilled water.
    3. Filter the mixture and collect the filtrate in a test tube.
    4. Prepare and label six cavities of a spot tile as follows:
    A: Amylase extract + 1 drop of distilled water
    B: Dilute hydrochloric acid (HCl)
    C: Amylase extract + 1 drop of dilute HCl
    D: Dilute sodium bicarbonate solution (Na2CO3)
    E: Amylase extract + 1 drop of dilute Na2CO3
    F: Distilled water
    5. Prepare six small filter paper discs.
    6. Put one filter paper disc into each of cavities A to F.
    7. Drain away excess liquid from the paper discs, and put them onto a starch-agar plate.
    8. Incubate the starch-agar plate at 40C for half an hour.
    9. Remove the paper discs from the plate. Flood it with iodine solution for 1 minute and then flush gently
    with tap water.
    10. Observe the colour changes.

    1. Why do we test the liquids in cavities A, C and E in the spot tile?


    To test the amylase activity at neutral, acidic and alkaline conditions respectively.
    2. Why do we test the liquids in cavities B, D and F in the spot tile?
    They act as control set-ups.
    3. Why do we incubate the agar plate at 40C instead of room temperature?
    It provides an optimum temperature for most enzyme-catalyzed reactions.
    4. In which medium does amylase work best?
    In an alkaline medium.
    5. From the results, give a conclusion for this experiment.
    Amylase works best in an alkaline medium.

    2
    Chapter 1: Life Processes─Enzymes

    Investigation Into the Effect of pH on the Activity of Catalase


    Effect of pH on Catalase
    ˙ A Hydrogen peroxide (H2O2) is a product of metabolism
    ˙ This product is poisonous for cells
    ˙ Enzyme catalase breaks down hydrogen peroxide.

    ˙ Potato cells contain a high concentration of catalase enzyme.


    Solutions
    ˙ Potato extract (contains catalse enzyme)
    ˙ pH buffer solution (repeat with different pH)
    ˙ 5% hydrogen peroxide solution
    ˙

    You might also like