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    AICE Biology: Using The Biuret Test To Detect The Presence of Peptide Bonds in A Solution

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    Wiji Ning
    Students will learn how to perform the biuret test to detect peptide bonds in solutions. The test involves adding biuret solution to solutions and observing any color change from pink to purple, which would indicate the presence of peptide bonds and thus proteins. It is a qualitative test. Students will then analyze their results to determine which solutions contained proteins based on the observations and biuret test results.

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    AICE Biology: Using The Biuret Test To Detect The Presence of Peptide Bonds in A Solution

    Uploaded by

    Wiji Ning
    53 views2 pages
    Students will learn how to perform the biuret test to detect peptide bonds in solutions. The test involves adding biuret solution to solutions and observing any color change from pink to purple, which would indicate the presence of peptide bonds and thus proteins. It is a qualitative test. Students will then analyze their results to determine which solutions contained proteins based on the observations and biuret test results.

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    AICE biology Biuret

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    Aice Biologybiuretlab

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    Students will learn how to perform the biuret test to detect peptide bonds in solutions. The test involves adding biuret solution to solutions and observing any color change from pink to purple, which would indicate the presence of peptide bonds and thus proteins. It is a qualitative test. Students will then analyze their results to determine which solutions contained proteins based on the observations and biuret test results.

    Copyright:

    © All Rights Reserved
    Download as DOC, PDF, TXT or read online from Scribd
    Download as doc, pdf, or txt
    53 views2 pages

    AICE Biology: Using The Biuret Test To Detect The Presence of Peptide Bonds in A Solution

    Uploaded by

    Wiji Ning
    Students will learn how to perform the biuret test to detect peptide bonds in solutions. The test involves adding biuret solution to solutions and observing any color change from pink to purple, which would indicate the presence of peptide bonds and thus proteins. It is a qualitative test. Students will then analyze their results to determine which solutions contained proteins based on the observations and biuret test results.

    Copyright:

    © All Rights Reserved
    Download as DOC, PDF, TXT or read online from Scribd
    Download as doc, pdf, or txt
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    AICE Biology: Using the Biuret Test to Detect the Presence of Peptide

    Bonds in a Solution
    Purpose:
    Students will learn how to perform the biuret test and interpret results to detect possible presence of
    peptide bonds in a solution.

    Materials:
    Each Lab Table should have the following: 6 labelled beakers set out, each with its own dropper.
    Solutions A, B, C , D, E and Biuret solution (bright blue)

    Each Lab group needs:


    1 test tube rack
    5 test tubes
    Sharpie marking pen

    Procedure:
    1. Label each test tube: A, B, C D, or E

    2. Place 2ml of solution into test tube A, then add 1ml drops of biuret solution. Swirl to mix & place
    in test tube rack.

    3. Repeat this procedure for each of your solutions.

    4. Observe colors of mixed solutions. A color change of pink to purple indicates the presence of
    peptide bonds. Be aware that the blue color of the biuret solution may cause a bluish tint to all
    solutions-even those with no peptide bonds.

    5. Record your data your data table.

    Data:
    Solution Initial Color Color after test Peptide bonds present?
    A
    B
    C
    D
    E

    Conclusions:
    1. Knowing that the biuret test detects the presence of peptide bonds, describe how this tells you
    whether proteins are present.

    2. Is this test quantitative or qualitative? Why

    3. Take a guess as to what you think was in each solution. Justify your answer for each based on
    observations of color, smell, biuret results, etc.
    LIPID Lab:

    Procedure:
    --Add a small amount of peanut butter (your lipid) to 2ml of ethanol in a screwcap tube
    --Cap the tube and shake VERY well.
    --Add 2ml of water to the lipid mixture and observe.

    Conclusions:
    DESCRIBE your observations. What does this tell you? Why does the lipid emulsify like this?

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