Tissue culture has several disadvantages: it is an expensive technique that requires expensive machinery, reagents, and highly trained personnel; it can reduce genetic diversity and introduce no new genes as all cloned plants are genetically similar; and if the original plant was susceptible to disease, the entire cloned stock will also be susceptible.
Tissue culture has several disadvantages: it is an expensive technique that requires expensive machinery, reagents, and highly trained personnel; it can reduce genetic diversity and introduce no new genes as all cloned plants are genetically similar; and if the original plant was susceptible to disease, the entire cloned stock will also be susceptible.
Tissue culture has several disadvantages: it is an expensive technique that requires expensive machinery, reagents, and highly trained personnel; it can reduce genetic diversity and introduce no new genes as all cloned plants are genetically similar; and if the original plant was susceptible to disease, the entire cloned stock will also be susceptible.
Tissue culture has several disadvantages: it is an expensive technique that requires expensive machinery, reagents, and highly trained personnel; it can reduce genetic diversity and introduce no new genes as all cloned plants are genetically similar; and if the original plant was susceptible to disease, the entire cloned stock will also be susceptible.
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Disadvantages of Tissue Culture:
The experiments involved in tissue culture are expensive
because the expensive machinery and reagents required, This technique is a complex procedure and it is has varied procedures depending on the plant type. The experiments of tissue culture must be handled by highly trained people as the procedure requires special are and careful observation. As all the plants are genetically similar, there is reduction is genetic diversity. There is no introduction of any new genes in the gene pool of the whole stock. If a plant is susceptible to disease, all the plants of this cloned stock will share this undesirable trait and be susceptible to that particular disease. On a large scale production tissue culture methods is very expensive as the cost of the equipments are very high. The procedure depends of the type of species being cultured; hence there is a need of trial and error method for any new species if there is no review about that species. Sometimes there is a possibility of error in the identity of the organism after culture, If precautions are not taken the whole stock may be contaminated or infected. Tissue culture, cloning decreases genetic variability. Monoculture is produced due to tissue culture and all the progeny may be vulnerable to the same infections or diseases. Samples of infected plants produce infected progeny; all the plants of the stock must be carefully screened to prevent culturing infected plants. Tissue culture is not successful with all plant species usually because of the growth medium and some plants produce secondary metabolites that might kill the explants. Some plants are difficult to disinfect of fungal infections.