Operations Manual

For Research Use Only

PH: 908-769-5555 EM: [email protected]

WEB: www.BenchmarkScientific.com
Thank you for choosing the BioClave Benchtop Sterilizer. TABLE OF CONTENTS
Prior to operating this instrument, please read the operations manual
carefully and follow all installation instructions.
1.GENERAL ------------------------------------------------------- 1
2.TECHNICAL SPECIFICATIONS --------------------------------- 2
3.PACKING CONTENT ------------------------------------------ 3
4.INSTALLATION ----------------------------------------------- 4
5.OPERATION ----------------------------------------------------- 5
5.1 SETUP -------------------------------------------------------- 5
5.2 PREPARATION OF STERILIZATION MATERIALS ----- 6
5.3 SELECTING THE STERILIZATION PROGRAM --------- 7
5.4 RUNNING THE STERILIZATION PROGRAM ------------ 8
6. ADVANCED SETTINGS --------------------------------------- 11
7. MAINTENANCE ----------------------------------------------- 15
8. TRANSPORT AND STORAGE ------------------------------- 18
9. ERROR CODES ------------------------------------------------ 19
10. SAFETY DEVICES ------------------------------------------- 20

APPENDIX
1.WATER PROPERTIES/CHARACTERISTICS ---------- 21
2. DIAGRAMS OF THE STERILIZATION PROGRAMS ----- 22
3.WIRING DIAGRAMS ----------------------------------- 25
4. HYDRAULIC DRAWING --------------------------------------- 26

Instructions manual Instructions manual

1 General 2 Technical Specifications
The sterilizer described in this manual is intended for the sterilization
of research tools. It operates automatically with 134°C and 121°C
sterilization temperatures. This sterilizer is in compliance with the Item 16
European Directive 93/42/CEE and it has been produced in accordance
with the EN 13060. In addition the chamber has been ASME certified. Chamber Φ230mmX360mm

Rated Voltage 110V-130V or 220V-240V, AC, 50-60Hz

1 11
Main Fuses F25A/250V for 110V
2 12
3 Nominal power 1900VA
13
Sterilization Temperature 121°C/134°C (250°F/274°F)
4 14

Capacity of the distilled Approx 2.5L (water at level MAX)

8 water tank
Approx 0.5L (water at level MIN)
9
7 6 5 15 16
10 Operation temperature 5 - 40℃
1 Distilled water tank 9 Printer port
10 Printer power
Exterior Dimensions 445mm(width)X 410mm(height)X 605mm(depth)
2 LED screen
3 Control panel 11 Bacteriological filter
4 Main Power switch 12 Safety valve 45kg
5 Drain connector of distilled water tank 13 Condenser ventilation
14 Circuit breaker
Weight
6 Door handle
7 Drain connector of used water tank 15 Power supply cord 99 lbs
8 USB port (optional) 16 Rating plate
Noise Level <70dB
Security Notice
For safe operation, please pay close attention to the alert symbols Relative Humidity max. 80%, non condensing
below which cab be found throughout this manual. Please
carefully read and understand the contents of this manual prior to Atmospheric pressure 76kPa -106kPa
operating this instrument.

This symbol represents an electrical caution - ground protection

HOT SURFACE.
This symbol represents a warning of a potential hot surface.

Important safety information.

This symbol represents a warning for extra caution

1 Instructions manual Instructions manual 2

3 Packing Content 4 Installation

Ensure that the sterilizer is installed with 2.5in. (10cm)

No. Item Quantity ventilation space on all sides of the sterilizer, and 5 in. (20cm)
1 Steam sterilizer 1 on top side. The clearance required to open the door is 15.5in.
2 Instrument tray (40cm).
2
3 Instrument tray rack 1 The sterilizer should be placed on a level worktable.
4 Instrument tray handle 1
Do not cover or block the door, ventilation or radiation
5 Door adjustment tool 1 openings on the sterilizer.
6 Draining hose 2
7 Instructions manual 1 Do not install the sterilizer near a sink or in a location where it
is likely tobe splashed.
8 Door seal 1
Do not install the sterilizer nearby a heat source.

605

3 Instructions manual Instructions manual 4

 5.1.4 Filling the distilled water
5 Operation Remove the cover, and fill the tank with distilled water.
5.1 Setup When you hear a beep signal, it means the water level exceeds the
5.1.1 Open the door and remove all of the inner contents for unpacking. max. level. The will be displayed. Please stop filling immediately.
5.1.2 Connect the power cord to an outlet of the appropriate voltage
5.1.3 Power on
The water level should not
The switch is located underneath the control panel exceeded this port.
on the front side of the machine .
After switching on, the machine turns on the LCD
and shows the door position, water level,
working program, date, time and etc.

5.2 Preparation of sterilization materials

Program
F Alarm code For the most effective sterilization and to preserve the sample, please
F
follow below:
process curve
* Arrange the samples of different material on different trays or with at least
Temperature 2in. of space between them.
F Setting
* Always insert a sterilization paper or cloth between the tray and sample,
Pressure Time
to avoid direct contact between the different materials.
Date

Distilled water tank is requires water.

Distilled water tank is full
Used water tank is full.
Door locked
information output to USB port
Printer is connected

Notice: Before using the sterilizer or at any time the low water
icon blinks, it is necessary to fill the distilled water
tank with distilled water.

5 Instructions manual Instructions manual 6

5.3 Selecting the sterilization program 5.4 Running the sterilization program.
5.3.1 LCD
The panel displays the cycle After selecting program, the materials
temperature, pressure, error code, to be sterilized can now be placed on the
sterilization state and program.
tray and the tray placed inside the
5.3.2 Temperature button chamber using the tray handle
Press this button to toggle
between 121°C and 134°C

5.3.3 Program button

Press this button to toggle between
available sterilization cycles (see below)
5.4.1 After the instruments are loaded, you
may close and lock the door by turning
5.3.4 START/STOP button the door handle clockwise until it stops.
Press this button to start the sterilization cycle. To stop a cycle, press
and hold this button for 3 seconds. The icon will be lightened.

Caution：You must turn the door handle to the maximum position,

otherwise the machine will alarm and prevent
completing the cycle.

UNWRAPPED
274 F
WRAPPED
DRYING

EXTENSIVE F

LIQUID

Notice: Button will be “locked” for the initial 10 seconds after power
up for system initialization.

7 Instructions manual Instructions manual 8

5.4.2 Start the sterilization program.
Press START button, the machine will begin the cycle automatically. Always use the tray handle to load or unload the tray into the
It will take 30-75 minutes. (See Appendix 2) autoclave. Failure to do so can result in burning.

If you need to interrupt a cycle and remove materials urgently.

you may hold the START button for 3 seconds after completing
sterilization time to skip the dry cycle.
This will result in the program skipping directly to the last step
and eliminate the drying stage. After one minute the cycle
will end.
F total time or
count down until
completion

Caution: When you press the START button but the door has not been
fully closed. You will see the blinking on the screen.
A cycle can not be started until you close the door to the max.
position and press the "Start" button again.

5.4.3 Sterilization cycle completion

After a cycle is completed, the printer will be activated and print out a
report of the cycle settings (if the optional printer has been connected).
After the pressure returns to 0, the door is unlocked and the materials
can be removed.

9 Instructions manual Instructions manual 10

6 Advanced Setting 6.4 S3 state
6.1 Enter the setting 6.4.1 Adjust the length of sterilization and drying time.
6.1.1 Power on the machine while Holding the
Press program button to select the program.( )
START button and hold for 5seconds. Thiw
will enter into the advanced settings mode. Press temperature button to select the temperature of program.
Then press START to adjust the drying time and holding time.
6.1.2 Select the state (State 1 thru. 3) by pressing 6.4.2 First to adjust the holding time.
the Program button. Press the START Press temperature button to adjust the data.
button to enter the setting.
Press the program button to select 274 F
the items.
6.2 S1 state
If you select the S1. You may change 6.4.3 Press START to save .
the unit of temperature and pressure, holding time
and adjust time and date. 6.4.4 Drying time is 0-19. drying time
6.2.1 The first option is to select the unit of Holding time of 250 F is 1-59.
temperature. Press temperature button
Holding time of 274 F is 1-19.
to select ℃ or F .
The unit you selected will be lighted. Press
the program button to the next item.
6.2.2 You may select the unit of pressure in the same manner. Notice: the default sterilization parameters have been chosen to
6.2.3 Then press program button to the next item to adjust the time and
date. After the last word of the date or time is set , then the data is provide optimal sterilization results. We do not suggest adjusting
permitted to be saved. If you want to finish the setting you shall press these parameters unless it is necessary.
START. It will return to the screen of selecting states.
6.3 S2 state
6.3.1 You may check the count of sterilization 6.5 Printer (Optional)
cycle. It can not be changed by operator. Altitude set
6.5.1 Connect the printer cable.
6.3.2 Set the parameter for high altitude. 6.5.2 Connect the printer power.
cycle No
If you have trouble completing a cycle in a
location of high altitude (above 2.0 km or
atmospheric pressure is below 80kPa)
you may need to adjust this parameter.
6.3.3 Language set:
00 English 01 German 02 Spanish
03 Polish 04 French 05 Magyar Language machine No
set
06 Romanian 07 Dutch 08 Lithuanian 09 Latvian

The Machine No. And cycle No can not be set by the operator.

11 Instructions manual Instructions manual 12

6.6 USB Flash memory (Optional) ========================= =========================
Program: WRAPPED Program: WRAPPED
A USB drive can be used as a method of storing a report of the Temperature: 274 F Temperature: 274 F
cycle. To do so, insert the USB drive to the slot on the right side of Pressure:30.5 psi Pressure:30.5 psi
the instrument. Dry Time: 03Min Dry Time: 03Min
Ster Time: 4.0Min Ster Time: 4.0Min
The information will automatically output directly to the USB ------------------------------------- -------------------------------------
after the cycle has completed. time temp. pressure time temp. pressure
The name of the file is determined by the serial number of Start 15:24:20 107.6 F Start 17:34:20 179.6 F
the machine and the cycle number. T1: 15:32:11 158.0 F 07.71psi T1: 17:42:11 194.0 F 07.57psi
For example : T2: 15:36:08 167.5 F 01.42psi T2: 17:46:08 185.5 F 01.41psi
T3: 15:39:21 194.5 F 07.30psi T3: 17:49:21 226.9 F 07.75psi
The serial number is E00001 . The cycle number is 00012 . T4: 15:44:32 094.3 F 01.39psi T4: 17:54:32 212.5 F 01.39psi
The file name in the USB stick is 0 1 0 0 1200 . txt . T5: 15:47:12 201.7 F 14.91psi T5: 00:00:00 000.0 F 000.0psi
The first two numbers represent machine number . T6: 16:00:11 230.3 F 01.35psi T6: 00:00:00 000.0 F 000.0psi
TS: 274.6 F 32.14psi TS: 000.0 F 000.0psi
The middle four numbers represent cycle number . Max.Temperature:275.2 F MAX.Temperature:000.0 F
The last two numbers represent error code . Min.Temperature:274.1 F MIN.Temperature:000.0 F
00 : no error ; 01 : error E01 Max.Pressure:33.42psi MAX.Pressure:000.0psi
Min.Pressure:30.88psi MIN.Pressure:000.0psi
6.7 Retrieve information from a prior cycle T7: 16:04:02 275.0 F 32.42psi T7: 00:00:00 000.0 F 000.0psi
T8: 16:06:32 274.6 F 31.05psi T8: 00:00:00 000.0 F 000.0psi
Press PROGRAM repeatidly until you enter
End 16:14:12 172.8 F End 17:54:42 212.4 F 01.46psi
the prior program storage screen. -------------------------------------- --------------------------------------
This will show the cycle No. Cycle No: 0005 Cycle No: 0007
Press the TEMP button to toggle between Ster Value: Success Ster Value: Failure E01
different cycles. Date: 2011-01-18 Date: 2011-01-18
SN:E00001 SN:E00001
To print or send details to the USB drive,
Operator: Operator:
press the START button. The most recent ======================= =======================
20 records are stored.
When vieing printed data records, refer to the diagram below:
P ts
t7 t8

t1 T3 t5

t
1 t2 t4 t6 2

13 Instructions manual Instructions manual 14

7 Maintenance 7.4 Clean the door seal
7. Maintenance Clean the door seal weekly, with a smooth cloth saturated with the
distilled water.
Frequency Maintenance Operation
Clean the door seal
Daily
Clean the external surface
Clean the distilled water tank
Weekly
Clean the sterilization chamber
Every year Replace the door seal

7.1 Clean the distilled water tank every 7.5 Door adjustment
week with isopropyl alcohol or a On normal circumstance the chamber door does not require adjustments.
medical disinfectant. However, if the seal fails (resulting in steam leaking from the front of
the chamber), you may use the included tool to tighten the door seal.
7.5.1 Open the door
7.5.2 Insert the spanner tool in the gap beneath the plastic cover; use the spanner
to grip the adjusting nut (Fig 1). Turn the nut counter clockwise as
the figure below (Fig 2). This will tighten the sealing plate.
7.2 Clean the chamber weekly. 7.5.3 Turn the nut until the sealing plate is tight. If the door knob is too tight,
7.2.1 Remove all trays and the tray rack you may also turn the nut clockwise to loosen it.
from the chamber.
7.2.2 Clean the chamber with a
smooth cloth saturated with
distilled water.
7.2.3 Apply the same procedure for
the trays and rack.

Fig 2
Fig 1

Caution: Never adjust the chamber door while the door is closed .

15 Instructions manual Instructions manual 16

7.6 Replacement of the door seal 7.7 The drain valve
7.6.1 Open the chamber door.
7.6.2 Remove the door seal ring carefully by hand.
7.6.3 Clean the door seal ring carefully with a smooth cloth
saturated with distilled water.
7.6.4 Moisten the new seal with medical disinfectant or
isopropyl alcohol. 2.Set the drain valve to the open
1.Press the included hose on to the position by turning it counter
drain valve firmly.
7.6.5 Insert the new seal and press in sequence as follows: clockwise.

3.Pull the drain valve outward, the 4.After finish draining the tank, push
tank will begin to drain. the drain valve inward and turn
clockwise into the closed position .
1). Press in the top and bottom 2). Press in the left and right sides
of the seal ring. of the seal ring.

8 Transportation and Storage

8.1 Switch off the sterilizer before transportation or storage. Pull out the
plug to let the machine cool down.
8.2 Drain the distilled water tank and the used water tank
8.3 Conditions for transportation and storage:
Temperature: -20 ~ +55

3). Press the remaining sections 4). Press all areas of the seal Relative humidity: ≥85%
of the seal ring. surface for a smooth finish. Atmospheric pressure: 50kPa~106kPa

Caution: Please ensure the chamber and the door have cooled
prior to replacing the seal ring.

17 Instructions manual Instructions manual 18

9 Error codes 10 Safety devices
(1)Main fuses
Protect the instrument against possible failures of the
Code Description Proposed solution
heating resistor .
Steam generator temperature Power off & run a new cycle
E1 sensor error Contact your Supplier if error persists Action: Interruption of the electric power supply.

Inner temperature sensor error Power off & run a new cycle
E2
Contact your Supplier if error persists (2)Thermal cutouts on the main transformer windings
Temperature sensor of chamber Carefully ensure that the chamber wall Protection against possible short circuit and main transformer primary
E3 wall error is heated. If not contact your supplier
winding overheating .
Fail to rise temperature Check to ensure that the used water
E4 valve is fully closed. Action: Temporary interruption of the winding.

Fail to release the pressure Power off & run a new cycle
E5 Contact your Supplier if error persists (3)Safety valve
Door has opened during the Make sure you have turned the door handle Protection against possible sterilization chamber over-pressure .
E6 cycle to the max. Position or check the door switch
Action: release of the steam and restoration of the safely pressure.
Ensure the distilled water tank isn't empty
E9 Failure to hold temperature Check the inner temperature sensor
Check the door for leaking

Check the steam generator heater

(4)Safety micro-switch for the door status
Failure to preheat the steam
E11 generator Check the steam generator protector Comparison for the correct closing position of the door .
Check the chamber heater Action: signal of wrong position of the door.
E12 Failure to preheat the chamber
Check chamber protector

Shut off the power and restart the

E20 Program manually interrupted (5)Door safety lock
power
Protection against accidental opening of the door.
Action: Impediment of the accidental opening of the door during the program.

(6)Self-leveling hydraulic system

Hydraulic system for the natural pressure levelling in case of manual cycle
interruption, Alarm or black-out .
Action: automatic restoration of the atmospheric pressure inside chamber.

19 Instructions manual Instructions manual 20

APPENDIX 1 APPENDIX 2
Water Properties/Characteristics DIAGRAMS OF THE STERILIZATION PROGRAMS

per tray ( kg )
Holding time

Max . Load
Temperature

Max . Load
Total time
Pressure

( kg )
DESCRIPTION FEED WATER CONDENSATE

( psi )

( min )
PROGRAM TYPE

( min )
( F)
。
Evaporate residue ≥10 mg/l ≥1.0 mg/kg
274 30.5 4 14 - 30
Silicium oxide sio 2 ≥1 mg/l ≥0.1 mg/kg UNWRAPPED Unwrapped solid 4 . 00 1 . 20
250 16.0 20 30 - 45 material
Iron ≥0.2 mg/l ≥0.1 mg/kg
274 30.5 10 30 - 55
LIQUID Liquid 1 . 20 0 . 40
Cadmium ≥0.005 mg/l ≥0.05 mg/kg
250 16.0 30 35 - 60

Lead ≥0.05 mg/l ≥0.1 mg/kg 274 30.5 4 30 - 50 Unwrapped porous material 4 . 00 1 . 20
WRAPPED
Rest of heavy metals, excluding Single - wrapped solid or
≥0.1 mg/l ≥0.1 mg/kg 250 16.0 20 35 - 55 hollow material
3.00 1 . 00
iron, cadmium, lead
Unwrapped porous material 1 . 00 0 . 30
Chloride ≥2 mg/l ≥0.1 mg/l
Single - wrapped porous
material
0 . 75 0 . 20
Phosphates ≥0.5 mg/l ≥0.1 mg/l Dual - wrapped porous
0 . 50 0 . 15
EXTENSIVE 274 30 . 5 18 35 - 55 material
Single - wrapped hollow
Conductivity (at 20℃) ≥15 μ s/cm ≥3 μ s/cm material
3 . 00 1 . 00

Dual - wrapped solid and

hollow material 2 . 00 0 . 75
pH value 5- 7. 5 5- 7
Colorless, clean, Colorless, clean, DRYING 1 - 20
Appearance without sediments without sediments

Hardness ≥0.02 mmol/l ≥0.02 mmol/l

The max. temperature of the 274 F sterilization cycle is 279 F

The max. temperature of the 0 F sterilization cycle is 256 F

21 Instructions manual Instructions manual 22

 Pressure(bar) Pressure(bar)
UNWRAPPED DRYING
2. 0 2. 0

1. 0 1. 0

Time(min) Time(min)
0. 0 0. 0

- 0. 8 - 0. 8

Pressure(bar) Pressure(bar) LIQUID

2. 0 WRAPPED 2. 0
DRYING
EXTENSIVE
1. 0 1. 0

Time(min) Time(min)
0. 0 0. 0

- 0. 8 - 0. 8

23 Instructions manual Instructions manual 24

APPENDIX 3 APPENDIX 4
WIRING DIAGRAM
HYDRAULIC DRAWING
USB DATA
OUTPUT

THERMAL
PROTECTOR
CHAMBER
HEATER Safety valve

STEAM
HEATER Pressure
THERMAL
PROTECTOR sensor
WATER
PUMP
VACUUM
PUMP
～230V(120V)

FAN PRINTER
Pump
OUTPUT

～ 9V(2.5A)
～ 0V
～ 9V(0.2A) TANK MIN. LEVEL Condenser Chamber
～21.5V PUBLIC
TANK MAX. LEVEL

+
USED WATER TANK
PRINTER
POWER
-

Distilled water tank

DOOR CLOSE
230V (120)

Tp3

V4
PRESSURE SENSOR Tp2
Used water tank
Tp1

V1

V1: Air release valve

KEYBOARD
DATA LINE
V4: Water release valve

TP1: Steam generator temperature sensor

TP2: Inner temperature sensor
TP3: Temperature sensor of chamber wall
V1: Air release valve
V4: Water release valve

25 Instructions manual Instructions manual 26