Determination of The Solution Color of Raw Sugars, Brown Sugars and Colored Syrups at PH 7.0 (According To ICUMSA Method GS1/3-7)

Application
Determination of the Solution Color of

Raw Sugars, Brown Sugars and
Colored Syrups at pH 7.0 (According
to ICUMSA Method GS1/3-7)
Method: Spectrophotometric Color Determination at 420 nm
Materials and Reagents:

Cat.No. 1.16754.9010 Water for analysis EMSURE® or distilled water
Cat.No. 1.09060.1000 Hydrochloric acid 0.1 mol/l Titripur®
Cat.No. 1.09141.1000 Sodium hydroxide solution 0.1 mol/l Titripur®
Cat.No. 1.14946.0001 Rectangular cells 10 mm or

Cat.No. 1.14947.0001 Rectangular cells 20 mm or
Cat.No. 1.14944.0001 Rectangular cells 50 mm
Cat.No. 1.73016.0001 Spectroquant® VIS Spectrophotometer Prove 100 or

Cat.No. 1.73017.0001 Spectroquant® UV/VIS Spectrophotometer Prove 300 or
Cat.No. 1.73018.0001 Spectroquant® UV/VIS Spectrophotometer Prove 600
Membrane filters, cellulose nitrate, pore size 0.45 µm, diameter 50 mm

Conical flasks 250 ml
Volumetric flask 500 ml
pH meter
Ultrasonic bath
Refractometer
Method:
Spectrophotometric Color Determination at 420 nm
Measuring range:
Method No. 2548
0 – 50 000 IU7.0
Working instructions:
Sample preparation
Mix the sample thoroughly.
Weigh quantities of sample and water as shown in “Table 1” below into a 250 ml conical flask.
Dissolve the sample by swirling at room temperature.
Adjust the pH of the prepared solution to 7.0 ± 0.1 with Hydrochloric acid 0.1 mol/l Titripur® or, respectively, Sodium
hydroxide solution 0.1 mol/l Titripur® as required.
Vacuum-filter the pretreated solution over a membrane filter into a clean and dry conical flask.
Degas the solution for 3 minutes in the ultrasonic bath.
Measure the % RDS (refractometric dry substance) of the solution to an accuracy of ± 0.1 g/100g (according to
ICUMSA Method GS4/3-13).
Merck KGaA, 64271 Darmstadt, Germany, Tel. 2017-02-14 Page 1 of 3

+49(0)6151 72-2440
EMD Millipore Corporation, 290 Concord Road,
Billerica, MA 01821, USA, +1-978-715-4321
Application
Table 1
Aliquots of sample and water (Water for analysis EMSURE® is recommended)
and recommended cell length
ICUMSA Color
Cell length Range Sample Water
[mm] [IU7.0] [g] [g]
<250 50 + 1 50 + 1
50 250 - 500 30 + 1 70 + 1
500 - 2000 30 + 1 70 + 1
500 - 2000 30 + 1 70 + 1
20 2000 - 7000 10 + 1 90 + 1
7000 - 25000 5.0 + 0.1 95 + 1
500 - 2000 30 + 1 70 + 1
10 2000 - 7000 10 + 1 90 + 1
7000 - 25000 5.0 + 0.1 95 + 1
>25000 5.0 + 0.1 95 + 1
Zero adjustment
For each measurement series, a zero adjustment is required.
It is recommended to use the same cell for zero adjustment and for sample measurement.
The zeroing procedure for the measurement series is automatically prompted by the instrument after entering the
%RDS value.
For zero adjustment fill a corresponding rectangular cell with distilled water or water for analysis.
After prompting, place the rectangular cell filled with water in the cell compartment, the zero adjustment is executed
automatically.
Confirm the implementation of zero adjustment with OK.
The zero adjustment is valid for the entire measurement series.
Measurement procedure

+49(0)6151 72-2440
Billerica, MA 01821, USA, +1-978-715-4321
Application
Select the method (2548, "ICUMSA Color GS1/3-7") from the method list of the Spectroquant® Prove.
Enter the RDS value in %. Confirm with OK.
Press START to start the measurement mode.
Perform a zero adjustment (procedure see “Zero adjustment”).
Transfer the sample solution into a corresponding cell. Place the cell into the cell compartment. The measurement
is performed automatically.
Confirm measurement with OK. The IU7.0 value is shown in the display.
Press START to start the measurement procedure for the next sample. The system does not prompt a repeat of
the zero adjustment.
Note:
The result is calculated according to ICUMSA GS1/3-7(2011) chapter 8.1 and ICUMSA SPS-4 table B (Sucrose;
20°C).
Individual ICUMSA methods or references are available online within 48 h: www.icumsa.org under “Methods
book”.
Short measuring procedures on Spectroquant® Prove instruments are described in the Analytical Procedures and
Appendices, available online on our website.
Ordering Information
Product Catalog No.
Prove 100 VIS Spectrophotometer 4 nm spectral bandwidth Spectroquant® 173016
Prove 300 UV/VIS spectrophotometer 4 nm spectral bandwidth Spectroquant® 173017
Prove 600 UV/VIS spectrophotometer 1,8 nm spectral bandwidth Spectroquant® 173018
Water for analysis EMSURE® 116754
Polypropylene Adapter 109060
Sodium hydroxide solution c(NaOH) = 0.1 mol/l (0.1 N) Titripur® Reag. Ph Eur 109141
Rectangular cells 50 mm Spectroquant® 114944
Rectangular cell 100 mm for Prove 600 Spectroquant® 174011

+49(0)6151 72-2440
Billerica, MA 01821, USA, +1-978-715-4321

Determination of The Solution Color of Raw Sugars, Brown Sugars and Colored Syrups at PH 7.0 (According To ICUMSA Method GS1/3-7)

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Application

Determination of the Solution Color of

Materials and Reagents:

Cat.No. 1.14946.0001 Rectangular cells 10 mm or

Cat.No. 1.73016.0001 Spectroquant® VIS Spectrophotometer Prove 100 or

Membrane filters, cellulose nitrate, pore size 0.45 µm, diameter 50 mm

Merck KGaA, 64271 Darmstadt, Germany, Tel. 2017-02-14 Page 1 of 3

Merck KGaA, 64271 Darmstadt, Germany, Tel. 2017-02-14 Page 2 of 3

Merck KGaA, 64271 Darmstadt, Germany, Tel. 2017-02-14 Page 3 of 3

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