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    Papanicolaou Staining Technique PDF

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    Kat Jornadal
    The Papanicolaou staining technique identifies stages of cell maturation through staining exfoliated epithelial cells. It uses hematoxylin to stain cell nuclei and orange green 6 or eosin azure to stain cytoplasm, allowing malignant cells to be identified. This technique can detect cancers from samples collected via spatula, brush, or other methods and evaluated from sites like the cervix, uterus, sputum, urine, or breast through microscopic examination.

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    Papanicolaou Staining Technique PDF

    Uploaded by

    Kat Jornadal
    543 views7 pages
    The Papanicolaou staining technique identifies stages of cell maturation through staining exfoliated epithelial cells. It uses hematoxylin to stain cell nuclei and orange green 6 or eosin azure to stain cytoplasm, allowing malignant cells to be identified. This technique can detect cancers from samples collected via spatula, brush, or other methods and evaluated from sites like the cervix, uterus, sputum, urine, or breast through microscopic examination.

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    The Papanicolaou staining technique identifies stages of cell maturation through staining exfoliated epithelial cells. It uses hematoxylin to stain cell nuclei and orange green 6 or eosin azure to stain cytoplasm, allowing malignant cells to be identified. This technique can detect cancers from samples collected via spatula, brush, or other methods and evaluated from sites like the cervix, uterus, sputum, urine, or breast through microscopic examination.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    543 views7 pages

    Papanicolaou Staining Technique PDF

    Uploaded by

    Kat Jornadal
    The Papanicolaou staining technique identifies stages of cell maturation through staining exfoliated epithelial cells. It uses hematoxylin to stain cell nuclei and orange green 6 or eosin azure to stain cytoplasm, allowing malignant cells to be identified. This technique can detect cancers from samples collected via spatula, brush, or other methods and evaluated from sites like the cervix, uterus, sputum, urine, or breast through microscopic examination.

    Copyright:

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    of 7

    PAPANICOLAOU

    STAINING
    TECHNIQUE
    PHT 413 15/18
    -diagnosis of malignancy thru microscopic
    examination of a single group or group of
    isolated cells has
    -staining method which identified stages in
    the maturation of exfoliated squamous
    epithelial cells
    -method with which malignant cells could
    be identified
    -detect human uterine and cervical cancers
    evaluation of sputum, urine, breast and
    other secretions
    Methods of Collecting a Pap Smear
    • Spatula
    • Cervical
    Brush
    • Spatula and
    Brush
    • Plastic
    “Broom”
    • Cotton Swab
    Stains used:
    – Harris Hematoxylin solution – nuclear stain; has
    affinity to the nucleus
    – Orange Green 6 (OG 6) – cytoplasmic stain; used
    to stain the cytoplasm of mature superficial cells)
    – Eosin Azure (EA) – used to stain the cytoplasm of
    immature cells (intermediates and parabasals).
    The stain contains a mixture of Eosin Y, Bismarck
    Brown and Light Green solution
    Three Commercial Eosin Azure
    Preparations
    Number represents the percentage
    of Light Green solution in each
    preparation
    • 1. EA 36
    • 2. EA 50
    • 3. EA 65
    Procedure:
    • 1. Fixation of smear in 95% ethanol.
    • Wash with water
    • 2. Primary staining with Hematoxylin
    • Wash with water
    • 3. Acid/alcohol for (regressive staining); skip this step
    in progressive staining
    • 4. Blueing with alkalinizing solution
    • 5. 1st counterstain using OG 6
    • Wash in 95% ethanol
    • 6. 2nd counterstain using EA
    • 7. Dehydrate, clear and mount.
    Precautions in Papanicolaou Staining:
    • 1. Leave the smear in solution (alcohol and
    water) until the liquid runs off the slides in a
    smooth clean sheet.

    • 2. Hematoxylin should be filtered daily before


    use. Smears should not be allowed to dry in
    between any of the staining steps.

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