CH 7 Notes Cellular Respiration

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Ch 7 notes

Cellular Respiration

 Primary aim is to make ATP and NADH. Primarily use glucose.

Four Metabolic pathways in cellular respiration.

1. Glycolysis

 Can occur with or without oxygen. Glucose is broken down into two pyruvate

molecules and yields two ATP and two NADH molecules. The ATP is

synthesized via substrate level phosphorylation. Glycolysis occurs in the

cytosol.

 Steps in glycolysis are nearly identical in all living species.

Ten steps in three phases:

 energy investment: 2 ATP hydrolyzed to create fructose, 6 biphosphate

 cleavage: 6 carbon molecules broken into 3 carbon molecules of

glyceraldehyde-3-phosphate

 energy liberation: Two glyceraldehyde-3-phosphate molecules broken down

into two pyruvate molecules- produces 2 NADH and 4 ATP.

2. Breakdown of pyruvate

 Two pyruvate molecules enter the mitochondrial matrix and each one is

broken down to one acetyl group and one CO2 molecule. One NADH is also

made. The total produced is 2 acetyl CoA, 2 CO2, and 2 NADH. Remaining

acetyl group attached to CoA to make acetyl CoA.

3. Citric acid cycle


 Each acetyl group is removed from acetyl CoA to form citrate and then

releases

 two CO2 molecules. One ATP, three NADH, and one FADH2 are made.

Process occurs in the mitochondrial matrix. Total is 4 CO2, 2 ATP, 6 NADH,

and 2 FADH2

4. Oxidative phosphorylation

 NADH and FADH2 release high energy electrons that go through an electron

transport chain to produce an H+ electrochemical gradient. Energy stored in

the gradient is used to synthesize ATP. 30-34 ATP are produced. Occurs

along cristae in eukaryotes and along the plasma membrane in bacteria and

archaea.

 Input: 10 NADH, 2 FADH2. Output: 30-34 ATP.

Oxidation by the Electron transport

 Protein complexes and small organic molecules embedded in the inner

mitochondrial membrane

 Accept and donate electrons in a linear matter in a series of redox reactions

 Movement of electrons generates an H+ electrochemical gradient.

 Provides energy for the next step synthesizing ATP.

 OXGYEN IS THE FINAL ELECTRON ACCEPTOR AND TURNED INTO

WATER

Metabolism of other macromolecules

 Fats and proteins can be used for metabolism

 Enter into glycolysis or citric acid cycle at different points.


 Utilizing the same pathways for breakdown increases efficiency

 Metabolism can also be used to make molecules

Fermentation

 Breakdown of organic molecules without net oxidation.

 In absence of oxygen need different way to produce ATP, like using glycolysis

 Glycolysis uses up NAD+ and makes too much NADH under anaerobic

conditions (dangerous situation)

 Muscle cells solve problem by reducing pyruvate into lactate (lactic acid

fermentation).

 Yeast solve problem by making ethanol.

 Fermentation produces less ATP than oxidative phosphorylation (alcohol

fermentation).

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