Chemistry Project
Chemistry Project
Chemistry Project
THEORY :
Wheat flour, gram flour, rice flour and potatoes
contains starch as the major constituent. Starch
present in these food materials is first brought
into solution.in the presence of enzyme
diastase, starch undergo fermentation to give
maltose. Starch gives blue-violet color with
iodine whereas product of fermentation starch
does’nt give any characteristic color. When the
fermentation is complete the reaction mixture
stops giving blue-violet color with iodine
solution. By comparing the time required for
completion of fermentation of equal amounts
of different substances containing starch the
rates of fermentation can be compared .
The enzyme diastase is obtained by
germination of moist barley seeds in dark at 15
degree celsius .When the germination is
complete the temperature is raised to 60
degree celsius to stop further growth. The seeds
are crushed into water and filtered. The filtrate
contains enzyme diastase and is called malt
extract.
MATERIALS REQUIRED :
Conical flasks
test tubes
filter paper and water bath
wheat flour
gram flour
rice flour
potatoes
1% of iodine solution.
PROCEDURE :
Take 5 gms of wheat flour in 100 ml conical flask
and add 30 ml of distilled water.
Boil the contents of the flask for about 5 minutes
Filter the above contents after cooling ,the
filtrate obtained is wheat flour extract.
To the wheat flour extract, taken in a conical
flask. Add 5 ml of 1% aq. NaCl solution.
Keep this flask in a water bath maintained at a
temperature of 50-60 degree celsius. Add 2 ml
of malt extract.
After 2 minutes take 2 drops of the reaction
mixture and add to diluted iodine solution.
Repeat the previous step after every 2 minutes.
When no bluish colour is produced the
fermentation is complete.
Record the total time taken for completion of
fermentation.
Repeat the experiment with gram flour
extract,rice flour extract, potato extract and
record the observations
OBSERVATIONS :
Time required for the fermentation—-
# Wheat flour — 10 hours
# Gram flour — 12.5 hours
# Rice flour — 15 hours
# Potato — 13 hours
BIBLIOGRAPHY :
http://en.wikipedia.org/wiki/Anise
www.chemicalland.com
www.laxmi.labinnapp.com
www.owlcation.com
CONCLUSION :
Rice flour takes maximum time for
fermentation and wheat flour takes the
minimum time for fermentation.