Biosystems - A25 Manual
Biosystems - A25 Manual
Biosystems - A25 Manual
SERVICE MANUAL
english
SERVICE MANUAL
English
TESE-0007-01-ING
October- 2008
TABLE OF CONTENTS
1. INTRODUCTION....................................................................7
1.1. GENERAL DESCRIPTION OF THE ANALYZER...............................................................7
1.1.1. Operating arm......................................................................................................................................... 8
1.1.2. Dispensing system................................................................................................................................ 8
1.1.3. Reactions rotor and reading................................................................................................................. 9
1.1.4. Electronic system................................................................................................................................ 10
1.1.5. Application program............................................................................................................................ 10
1.2. FUNCTIONING OF THE ANALYZER...............................................................................10
1.3. TRANSPORT AND RESHIPMENT OF THE ANALYZER................................................11
2. Electronic system and fluids.............................................13
Description of the electronics of the A25 analyzer.............................................................13
2.1 CPU Board (CIIM00006)...................................................................................................13
2.2 Power Supply Board (CIIM00007)...................................................................................19
2.3 Needle Board (CIIM00008)...............................................................................................21
2.4 Photometry Board (CIIM00009).......................................................................................22
2.5 Racks Board (CIIM00010)................................................................................................23
2.6 LED Board (CIIM00011)....................................................................................................23
2.7 Communications Board (CIIM00019)..............................................................................24
2.8 List of components..........................................................................................................25
2.9 Auxiliar channel information...........................................................................................25
2.10 Boards interconnection . ..............................................................................................30
2.11 Schematic liquid circuit.................................................................................................39
3. SERVICE PROGRAM..........................................................40
3.1 Initialising the analyser...................................................................................................40
3.2. ADJUSTMENTS ..............................................................................................................41
3.2.1. Adjustment of the needle thermostatation system........................................................................... 42
3.2.2. Adjustment of the rotor thermostation system................................................................................. 43
3.2.3. Adjustment of the positioning of the operating arm......................................................................... 43
3.2.3.1 Adjusting the maximum sweep of the Z axis................................................................................... 44
3.2.3.2 Fine adjusting the maximum sweep of the Z axis........................................................................... 45
3.2.4. Adjustment of the positioning of the rotor........................................................................................ 46
3.2.4.1. Centering of the rotor with regard to the dispensing point.......................................................... 46
3.2.4.2. Centering of the rotor with regard to the optical system.............................................................. 46
3.2.5 . Adjustment of the positioning of the filter wheel............................................................................. 47
3.2.6. Adjustment of the level control scales............................................................................................... 48
3.2.7. Adjustment of the level detection sensitivity.................................................................................... 49
3.3. TESTS...............................................................................................................................49
3.3.1. Motor tests............................................................................................................................................ 49
3.3.1.1. Initialization test................................................................................................................................ 50
3.3.1.2. Movement test................................................................................................................................... 51
3.3.1.3. Loss step test.................................................................................................................................... 51
3.3.1.4. Stress mode test............................................................................................................................... 52
3.3.1.5. Z axis security systems test............................................................................................................ 52
3.3.1.6 Maximum Z verification test.............................................................................................................. 52
3.3.2. Diaphragm pumps and electrovalves test......................................................................................... 53
3.3.2.1. Functioning test................................................................................................................................ 53
3.3.2.2. Stress mode test............................................................................................................................... 53
3.3.3. Needle self-centering system test...................................................................................................... 54
Service Manual
A I. TECHNICAL SPECIFICATIONS.......................................76
A II. ADJUSTMENT MARGINS TABLES.................................81
A III. LIST OF ACCESSORIES AND SPARES........................82
A IV. LIST OF REQUIRED TOOLS..........................................87
A V. SOFTWARE VERSIONS..................................................88
1. INTRODUCTION
The A25 analyzer is an automatic random access analyzer specially designed for performing biochemical and turbi-
dimetric clinical analyses. The instrument is controlled on-line in real time from an external dedicated PC.
In each of the components of the A25 analyzer, BioSystems has used leading edge technology to obtain optimum
analytical performance, as well as taking into account economy, robustness, easy use and maintenance. A three-axis
Cartesian operating arm prepares the reactions. Dispensing is performed by means of a pump with a ceramic piston
via a detachable thermostatised needle with Fuzzy Logic control. A washing station guarantees that the needle is
kept perfectly clean throughout the process. The reactions take place in a thermostatised rotor in which absorbance
readings are taken directly by means of an integrated optical system.
This manual contains the information required for learning about, maintaining and repairing the A25 automatic analy-
zer. It should be used by the Technical Service as a learning and consultation document for the maintenance and
repair of the instrument. Chapter 2 describes the different mechanical components that form the analyzer together
with their functionality, and chapter 3 describes the electronic system. Chapter 4 describes the Service Program.
All the adjustments and checks of the analyzer are carried out through this program, which is independent from the
application program (User Program). The separation of both programs enable it to be maintained separately and the
extensions and improvements of one do not affect the other. The user does not have the service program. The Te-
chnical Service must install it on the user’s computer in order to carry out the service requirements. Once said tasks
have been carried out, the Technical Service must uninstall the program. Chapter 5 offers instructions for the different
maintenance, repair and cleaning operations that can be carried out by the Technical Service. The annexes contain
a summary of the technical specifications of the analyzer, the adjustment margin tables, the lists of accessories and
spares, a list of software versions and their compatibility and a software troubleshooting guide.
The A25 analyzer is made up of three basic components: the operating arm, the dispensing system and the reading
and reactions rotor. The electronic system of the instrument controls said components and communicates with the
external computer containing the application program. Through this program, the user can control all the operations
of the analyzer. The analyzer may be fitted with the option of an external module for measuring ion concentration.
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Service manual
This is a three-axis XYZ Cartesian mechanism. The X and Y axes move the dispensing needle over the analyzer
horizontally and the Z axis moves it vertically. It is operated by three step-by-step motors. In each 15-second prepara-
tion cycle, the operating arm performs the following actions: first of all, it sucks in the reagent from the corresponding
bottle. Next, the needle is washed externally in the washing station and sucks in the sample from the corresponding
tube. It is washed externally again and dispenses the sample and the reagent into the reactions rotor. Finally, it is
exhaustively washed internally and externally before proceeding with the next preparation. The arm has a system
for controlling vertical movement to detect whether or not the needle has collided into anything on descending. If a
collision occurs, as may be the case if, for example, a lid has been left on a bottle of reagent, the arm automatically
restarts, verifies the straightness of the needle and continues working issuing the corresponding alert to the user.
A vertical axis retention system prevents the needle from falling in the case of a power cut, avoiding injury from the
needle to the user or the needle being bent by an attempt to move the arm manually. The operating arm only makes
the preparations if the general cover of the analyzer is closed. If the cover is raised while it is functioning, the arm
automatically aborts the task in progress and returns to its parked position to avoid injury to the user.
This system consists of a thermostatised needle, supported and displaced by an operating arm and connected to a
dispensing pump. The needle is detachable to enable cleaning and replacement. The analyzer has capacity level
detection to control the level of the bottles and tubes and prevent the needle from penetrating too far into the corres-
ponding liquids, thus minimising contamination. An automatic adjustment system informs the user if the needle is
not mounted or if it is too bent. The needle has a sophisticated Peltier thermostatisation system, with Fuzzy Logic
control, capable of thermostatising the preparations at approximately 37º in less than 6 seconds. Dispensing is carried
out by means of a low maintenance ceramic piston pump driven by a step-by-step motor. It is capable of dispensing
between 3 and 1250 µL. The exterior of the needle is kept constantly clean by means of a washing station, which
consists of a font specially designed to clean and dry the needle, integrated in the racks tray. A system of diaphragm
pumps supplies the font with distilled water and transports the waste to its container.
The A25 analyzer has a tray with 3 free positions for racks of reagents or samples, plus three fixed positions for bottles
opposite the washing station. Each reagents rack can carry up to 10 reagents in 20 ml or 50 ml bottles. Each sam-
ples rack can contain up to 24 tubes of samples. The samples can be patients, calibrators or controls. The analyzer
can be configured to work with 13 mm or 15 mm diameter tubes of samples with a length of up to 100 mm or with
paediatric wells.
Any possible configuration of racks can be mounted from 3 racks of samples (72 samples) and 30 refrigerated bottles
of reagent to 2 racks of unrefrigerated reagents (20 reagents) and 30 refrigerated bottles of reagent and 1 rack of
samples (24 samples). Any reagent may be placed in the fixed positions, but it is recommendable to use them for the
bottles of distilled water, saline solution for the automatic pre-dilutions and washing solution. The rack tray detects and
8
identifies the type of racks. In this way, if the physical disposition of the racks does not coincide with that programmed
on the comp uter, the analyser alerts the user.
On the left of the analyser are system liquid containers (marked in blue) and waste containers (marked in red), and
on the back of the analyser is connected the bottle of washing solution (marked in green). The analyser constantly
controls the level of these containers and issues the appropriate alerts if the system liquid or washing solution is nearly
empty or if the waste container is full.
The preparations are dispensed in an optical quality methacrylate reactions rotor thermostatised at 37ºC. The optical
absorbance readings are taken directly on this rotor. Each reaction can be read for 15 minutes. The readings are taken
as they are programd in each measurement procedure. The reaction wells have been designed to enable the mixture
of the sample and the reagent during the dispensing. Each rotor has 120 reaction wells. The length of the light path is
6 mm. The minimum volume required to take the optical reading is 200 µL. The wells have a maximum useful capacity
of 800 µL. When the reactions rotor is completely full, the user must change it with one that is empty, clean and dry.
The reactions rotors can be reused up to 5 times if they are carefully cleaned immediately after use. The Cleaning
the semi-disposable reactions rotor section in the User’s Manual describes how to clean the rotors. The user has a
test in the computer program, which he or she may use to check the condition of the rotor. The rotor is driven by a
step-by-step motor with a transmission. A Peltier system with PID control thermostatises the rotor at 37ºC.
An optical system integrated in the rotor takes the readings directly on the reaction wells. The light source is a 20 W
halogen lamp. The detector is a silicon photodiode. The wavelength is selected by a wheel with 9 positions available
for optic filters. The filters are easily changed by the user from the exterior of the analyzer, without the need for disas-
sembling the filter wheel. A step-by-step motor positions the wheel. The optical system is capable of taking 5 readings
per second, with or without a filter change in between. The light beam of the lamp passes through a compensated
interferential filter to select the desired wavelength and through focalisation lenses. It then passes through the rotor well
and finally reaches the photodiode, where the light signal is turned into an electric signal. A sophisticated analogical
digital integrator-converter system converts the electric signal into a digital value with which the analyzer obtains the
absorbance values. The optical system continues to work when the general cover of the analyzer is open, whereby the
analyzer can continue to take readings while the user handles, for example, the sample tubes or the reagent bottles.
The rotor cover must be in placefor the optical system to work correctly. A detector tells the analyzer of the presence
of the cover. The analyzer aborts the readings if the user removes the rotor cover while the optical system is taking
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Service manual
photometric measurements. If the rotor is not covered, the analyzer informs the user so that he or she places the rotor
cover when it sends samples to be analyzed.
The described components are controlled by an electronic system based on a microprocessor. The microprocessor
has two external communication channels that make it possible to link up the instrument to the computer containing
the application program and to an optional external module for measuring ion concentration. The electronic system
is made up of the following independent boards:
- Microprocessor board
- Photometric system board
- Needle conditioning board
- Racks detection board
- Front indicator board
- Power supply board
- Communications board
The application program makes it possible to control all the operations of the analyzer. From this program, the user
can monitor the state of the analyzer and the work session, program parameters, e.g. technique parameters, prepare
the work session, prepare results reports, configure different analyzer options, activate various test utilities, prepare
and maintain the instrument and carry out internal quality control processes. The purpose of this manual is not to
explain the functioning of the user program. For detailed information to this regard, please consult the User’s Manual
included with the analyzer.
10
1.2. FUNCTIONING OF THE ANALYZER
The A25 analyzer is an automatic random access analyzer specially designed for performing biochemical and turbi-
dimetric clinical analyses. The analyzer performs patient-by-patient analyses and enables the continual introduction
of samples. The analyzer is controlled from a dedicated PC that is permanently communicated to the instrument. The
program, installed on the computer, keeps the user constantly informed of the status of the analyzer and the progress
of the analyses. As results are obtained, the computer shows them to the user immediately.
When a Work Session is begun, the analyzer proposes performing the blanks, calibrators and controls programd for
the measurement procedures it is to carry out. The user may choose between performing the blanks and the calibra
tors or not. If they are not performed, the analyzer uses the last available memorised data. The controls can also be
activated or not. During a session, while the analyzer is working, the user can introduce new normal or urgent samples
to be analyzed. Each time a new sample is added, the analyzer automatically proposes the possible new blanks, cali-
brators or controls to be performed. A work session can remain open for one or more days. When a session is closed
and another new session is opened (Reset Session), the analyzer again proposes performing the blanks, calibrators
and controls. It is recommended that the session is reset each working day.
The analyzer determines the concentrations of the analytes based on optical absorbance measurements. To measure
the concentration of a certain analyte in a sample, the analyzer uses a pipette to take a specific volume of the sample
and the corresponding reagent, quickly thermostatises them in the needle itself and dispenses them into the reactions
rotor. The very dispensing speed together with the geometry of the reaction well causes the mixture to be shaken and
the chemical reaction begins. In the bireagent modes, the reaction begins when the analyzer later dispenses a second
reagent in the same reaction well. The reactions can be biochemical or turbidimetric. In both cases, the reaction or
the chain of reactions produced generate substances that attenuate certain wavelengths, either by absorption or by
dispersion. Comparing the light intensity of a certain wavelength that crosses a well when there is a reaction and when
there is not a reaction can determine the concentration of the corresponding analyte. This comparison is quantified with
the physical magnitude called absorbance. In some cases, the concentration is a direct function of the absorbance,
and in other cases, it is a function of the variation of the absorbance over time, depending on the analysis mode.
If the analyzer is to be reshipped or moved using a transport vehicle, it is important to block the operating arm and
use the original packaging to ensure that the apparatus is not damaged. To package the instrument, we recommend
you follow the following instructions: (on the unpackaging instructions sheet).
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Service manual
4
Unblock the operating arm taking
out the screws (1), (2), (3) and the
l-braket (4).
12
2. Electronic system and fluids
1. CPU Board (CIIM00006)
2. Power Supply Board (SP300 & CIIM00007)
3. Needle Board (CIIM00008)
4. Photometry Board (CIIM00009)
5. Racks Board (CIIM00010)
6. LED Board (CIIM00011)
7. Communications Board (CIIM00019)
8. List of components
9. Auxiliar channel information
10. Interconnection between boards
11. Schematic liquid circuit
The electronics of the analyzer are made up of different boards located at different points in the analyzer and dedicated
to specific functions. Their different locations correspond to functionality and performance criteria for the functioning
of the analyzer.
This is the brain of the machine, containing the microprocessor (H8/3003), responsible for controlling all the components
of the machine. The board has different data storage systems using either static RAM (U21), FLASH memory (U18) or
EPROM (U20). The slot associated with the EPROM is used to check the functionality of the board and the recording
of the MONITOR program in the production phases of the analyzer. The other two memories are associated with the
normal functioning of the analyzer. The FLASH memory holds the application itself as well as different databases
related to factory settings, adjustments, state of the rotor and possible extensions to the application.
The U34 device also exists on the board. This is a logical programmable device (FPGA) dedicated to the control of
motors, mapped in register memory associated with end-of-run control, electrovalves, decoding of racks (CIIM00010),
level sensing and control of the photometry-associate board (CIIM00009).
The control of the motors acts directly with the L6208 driver. There are one driver for each motor. The regulation of
the current of each axis can be configured by means of a DAC that sets the current set point independently (U5). The
sequencers are supplied through the U17 regulator and the drivers take their supply through the J16 connector, which
corresponds to the 36V input on the CIIM00006 board.
The action on the thermostatation systems of the analyzer (needle and rotor) is carried out through H-shaped bridges
based on MOS technology (U29 and U30) and controlled directly from the microprocessor. The heating elements are
connected to J27 (needle) and J28 (rotor), respectively.
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Service manual
J1 Motor Z 1 - coil 1
2 - coil 1
3 - coil 2
4 - coil 2
J2 Motor Y 1 - coil 1
2 - coil 1
3 - coil 2
4 - coil 2
J4 Motor X shaft 1 - coil 1
2 - coil 1
3 - coil 2
4 - coil 2
J5 Pump Motor 1 - coil 1
2 - coil 1
3 - coil 2
4 - coil 2
J6 Filter Drum Motor 1 - coil 1
2 - coil 1
3 - coil 2
4 - coil 2
J7 Rotor Motor 1 - coil 1
2 - coil 1
3 - coil 2
4 - coil 2
J8 Diaphragm inlet Pump 1 - motor
2 - motor
J11 Diaphagm outlet pump 1 - motor
2 - motor
J9 3-way electrovalve 1 - coil 1
2 - coil 1
J10 3-way electrovalve 1 - coil 1
2 - coil 1
J12 3-way electrovalve 1 - coil 1
2 - coil 1
J13 2-way electrovalve 1 - coil 1
2 - coil 1
J15 The signal enabling level detection by the analyzer
needle is injected into the racks tray through this con- 1- Faston
nector.
J16 36V voltage 1 - 36V
2 - GND
J17 12V voltage 1 - GND
2 - 12 V analogical
3 - GND
4 - 12 V peltier
5 - GND
6 - 12 V valves
14
Connector Function Pins
1 - Vdc
2 - Rotor cover
3 - Home rotor
4 - GND
5 - Vdc home rotor
J24 Rotor connector 6 - Filter wheel home
7 - GND
8 - Vdc Filter wheel home
9 - Rotor thermostatation
10 - GND
11 - Analogic 12 V
12 - GND
1 - Vdc
2 - GND
3 - Tx0
4 - GND
J25 Communications Board Connection (CIIM00019) 5 - Rx0
6 - GND
7 - GND
8 - Tx1
9 - GND
10 - Rx1
J27 Needle Peltier 1 - peltier, red
2 - peltier, black
J28 Rotor Peltier 1 - peltier, red
2 - peltier, black
1 - GND
2 - Home Z
J29 Needle Board Interconnection (CIIM00008) 3 - Needle thermistor
4 - Needle level detector
5 - GND
6 - 12 V
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Service manual
1 - Encoder detector
J30 Z axis encoder
2 - GND
3 - Vdc
1 - Home
J31 X axis home
2 - GND
3 - Vdc
1 - Home
J32 Y axis home
2 - GND
3 - Vdc
1 - Home
J33 Ceramic pump home
2 - GND
3 - Vdc
1 - Detector 1
2 - Detector 2
.
J34 Rack decoding board connection (CIIM00010)
.
24 - Detector 24
25 - GND
26 - Vdc
1 - 12 V
2 - GND
3 - DVALID
4 - DCLK
5 - DOUT
6 - DXMIT
7 - RANGE2
J35 Photometric board connection (CIIM00009) 8 - RANGE1
9 - RANGE0
10 - TEST
11 - CONV
12 - GND
13 - CLKAD
14 - GND
15 - GND
16 - Vdc
1 - GND
J36 LED board connection (CIIM000011) 2 - LED
3 - LED
4 - Vdc
J99 Fan 1 - 12V
2 - GND
16
Connector Function Pins
1 - 5V
Connection of I2C bus to the power supply board 2 - SDA
J40
(CIIM0007)
3 - CLK
4 - GND
1 - 5V
2 - GND
3 - White
J41 Connection to the photodetectors of probe detection. 4 - Green
5 - 5V
6 - GND
7 - White
8 - Green
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Service manual
As for analogical circuitry on the board, the J19 connector corresponds to the input of the sensors for machine water
and waste control. These sensors are load cells and they are conditioned by U16 and associated components. The
sensor signal is linearised and amplified and is then redirected to the analogical-digital converters in the microproces-
sor. There is also a circuit for conditioning the signal of the thermistor associated to the thermostatation of the rotor
that is made up of the U22 and U28 circuits. The signal used to detect the level is generated by U28. The injection
of the signal is done by J15 and it is collected through the cables that come from the analyzer needle and connect
on J29.
There are also different integrated circuits for the encoding of the control signals for all the components that share
the data and address bus (U26, U23, U24 and U36), control signals inversion (U35).
TP1 - VRAM
TP3 - LOW-LINE
TP4 - RESET
TP6 - WATCHDOG
TP12 - Needle level detector
TP13 - LSO
TP14 - RW
TP20 - CS_EPROM_L
TP21 - CS_RAM_L
TP22 - CSF2_L
TP23 - CSF1_L
TP24 - RD_L
TP25 - CLK system (16 MHz)
TP28 - LWR_L
TP29 - HWR_L
TP30 - Motor chopper frequency
TP36 - Conditioned signal output from the rotor thermistor
TP37 - Analyzer cover
TP39 - Rotor cover
TP40 - CS_FPGA_L
TP44 - INIT_L
TP45 - DONE
TP46 - PROGRAM_L
TP47 - CCLK
TP48 - DIN
TP50 - Z shaft motor vref
TP51 - Y shaft motor vref
TP52 - X shaft motor vref
TP53 - Dispensation/aspiration pump motor vref
TP54 - Filter drum motor vref
TP55 - Rotor motor vref
TP56 - IN1 Needle Peltier Driver
TP57 - EF Needle Peltier Driver
TP58 - IN2 Needle Peltier Driver
TP59 - IN1 Rotor Peltier Driver
TP60 - EF Rotor Peltier Driver
TP61 - IN2 Rotor Peltier Driver
18
2.2 Power Supply Board (CIIM00007)
This is made up of 5 different switched voltage regulators that enable distribution of the power supply in accord with
the requirement of each subsystem.
The existing bridge is to enable the different supply voltages. It has the same function as the enable of the J1 con-
nector.
D3 - Indicates 5V activated
D4 - Indicates 12V lamp activated
D7 - Indicates 12V valves activated
D8 - Indicates 12V analogicals activated
D10 - Indicates 12V thermos activated
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Service manual
20
2.3 Needle Board (CIIM00008)
This board conditions the thermistor signal associated with the thermostatation of the needle, the preamplification of
the level detection signal and the Z home. It receives, from the needle-fan unit associated with thermostatation, the
Peltier cell, the thermistor and the level signal detected by the needle itself.
Two sets of cables leave this needle and join this board with the CIIM00006-01 board (specifically with the J29 and
J27 connectors) for its J1 and J2 connectors.
1 - Fan control
2 - Home Z
J1 CPU board connection (CIIM00006) 3 - Thermistor
4 - Sensor level
5 - GND
6 - 12V
1 - Peltier
J2 CPU board connection (CIIM00006) for the Peltier
2 - Peltier
3 - Chassis
J3 Thermistor 1 - GND
2 - Thermistor
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Service manual
1 - 12 V
2 - GND
3 - DVALID
4 - DCLK
5 - DOUT
6 - DXMIT
7 - RANGE2
8 - RANGE1
J3 Photometric board connection (CIIM00009)
9 - RANGE0
10 - TEST
11 - CONV
12 - GND
13 - CLKAD
14 - GND
15 -GND
16 - Vdc
JP1 - soldering bridge - Solder only if the local oscillator and the U4 and U5 flip-flop, respectively, are not present.
JP2 - soldering bridge - The same than JP1
JP3 - soldering bridge - joins together the analogical and digital ground
22
2.5 Racks Board (CIIM00010)
This board decodes the rack type the analyzer has at each of the positions for said racks. It is made up of a battery
of photobarriers that allow or stop light passing through the rack identification tabs at logical levels the analyzer fir-
mware can handle.
This board indicates the different states of the analyzer. It is made up of a bicolor LED and the circuitry associated
with action.
D1 - Bicolor LED
1 - GND
J36 LED board connection (CIIM000011) 2 - LED
3 - LED
4 - Vdc
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Service manual
This enables communication with the exterior of the analyzer through a USB channel or a RS232 channel. It also
includes an auxiliary RS232 channel for monitoring the functions of the analyzer during its execution.
1- Vcc
2- GND
3- TX0
4- GND
5- RX0
J1
CPU board connection (CIIM0006) 6- GND
7- GND
8- TX1
9- GND
10- RX1
24
2.8 List of components
Component Reference
2 way electrovalve SIRAYD 10302.Z031C 12VCC
3 way electrovalve LVM115-6A-2U-1 from SMC
Cell load DF2SR-3 20K
Cover magnet Neodimio D4x5
Electronic box fan SUNON KDE1208PTV113.MS.A.GN
Filter wheel motor NMB23ML-C343V-1
Hall efect sensor RELE REED A041 1D 2H0500
Home detector TCST1300
Lamp 12V 20WG4 BLV
Membrane pump MRL12VDC
Probe fan NMB2404 KL-04W-B40
Probe temperature sensor 2.2K3A1iAM
Pump motor NMB23ML-C343V-1
Rotor fan SUNON KDE1208PTV113.MS.A.GN
Rotor motor NMB23ML-C343V-1
Rotor peltier CP1.0-127-05L-1-EP
Rotor temperature sensor 2.2K3A1iAM
X motor HY-200-2226-0160-AX08
Y motor NMB23ML-C343V-1
Z motor NMB17PMKD18V
The rear left part of the instrument is where the communications cables are connected. There are two connections,
the COM1 and the COM2.
The COM1 is the main connection from the analyser to the computer. This connection should be always present to
analyser run propertly.
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Service manual
Onces is configured and connected the cable, switch on the analyser. In this moment will appear in the HyperTerminal
screen information about the analysers mode and the different executes states. In the initializate mode, the analyser
do an internal checking for each element, if someone has any error then in the screen will show the element that fails.
The following lines shows an exemple of the instructions during an initialization, (this information could change with
the improvements of the firmware) :
- Forced Monitor?: No
- Monitor Mode?: No
- Checking User Firmware: Found Firmware
Checksum Ok
- Launching User Firmware!
[A25V2]
Firmware initialization
Firmware Version: A25 User V3.30
Serial Number: 831013000
26
Configuration in FLASH is correct
Adjustments loaded:
Filter 1=000 Integration Time= 10ms ( 98) Reference Time= 10ms ( 97)
Filter 2=340 Integration Time= 70ms (684) Reference Time= 70ms (683)
Filter 3=405 Integration Time= 18ms (178) Reference Time= 18ms (177)
Filter 4=505 Integration Time= 17ms (163) Reference Time= 16ms (161)
Filter 5=535 Integration Time= 16ms (153) Reference Time= 15ms (151)
Filter 6=560 Integration Time= 18ms (179) Reference Time= 18ms (177)
Filter 7=600 Integration Time= 18ms (179) Reference Time= 18ms (178)
Filter 8=635 Integration Time= 20ms (193) Reference Time= 20ms (191)
Filter 9=670 Integration Time= 23ms (228) Reference Time= 23ms (226)
Filter 10=000 Integration Time= 10ms ( 98) Reference Time= 10ms ( 97)
TI/LB Hystoric:
F[01]: 00004018 00004046 00004064 00004008 00004000 00004051 00004042 00004111 00004060 00004029
F[02]: 00616499 00615007 00622104 00618388 00610488 00608066 00613470 00597748 00601531 00609393
F[03]: 00897499 00898168 00898858 00897966 00895314 00896380 00899938 00896324 00897692 00896351
F[04]: 00895331 00894947 00895349 00894860 00898594 00897303 00895865 00897859 00896842 00898261
F[05]: 00898856 00898348 00898632 00898263 00897672 00895180 00899704 00896637 00894443 00897725
F[06]: 00897651 00896160 00898222 00897234 00899144 00895317 00896807 00895461 00899144 00896267
F[07]: 00899631 00898217 00897954 00897230 00897751 00899972 00896751 00896357 00898677 00898857
F[08]: 00896040 00899758 00897740 00896050 00897510 00899633 00897053 00897614 00899586 00898564
F[09]: 00897553 00897843 00897142 00899308 00898354 00899756 00896360 00899912 00899935 00897069
F[10]: 00003993 00004052 00004066 00004014 00004033 00004010 00004017 00004052 00004023 00004029
F[01]: 098 098 098 098 098 098 098 098 098 098
F[02]: 684 684 684 684 684 684 684 684 684 684
F[03]: 177 178 176 177 179 180 179 183 182 178
F[04]: 161 162 160 161 165 165 163 167 166 163
F[05]: 152 153 151 152 155 155 154 157 156 153
F[06]: 178 179 177 178 182 182 180 184 184 179
F[07]: 180 181 179 180 181 183 181 185 185 179
F[08]: 193 195 192 193 196 198 195 200 200 193
F[09]: 229 231 228 230 232 234 231 237 237 228
F[10]: 098 098 098 098 098 098 098 098 098 098
Zmax Reference=170
- Pediatric Offset=-276
- 13mm Offset=1
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Service manual
- 15mm Offset=0
- Reagent Offset=4
- Central Reagent Offset=0
- Fridge Reagent Offset=16
A25 Statistics
Tray Ref. X=1345 => Distance from tray reference to tray corner X=1082
Tray Ref. Y=344 => Distance from tray reference to tray corner Y=432
BioSystems A25
Hello World
H: Help
R: Rotor Temperature
P: Probe Temperature
S: Level Scales
A: Last A25 Stress Results
L: Actual Sensitivity of Level Detection
N: Enable Level Detection Debug
Buzzer Control
B: Buzzer On
b: Buzzer Off
Encoder
Y: A15 Encoder profile (Press while booting)
Rotor Reading
1: Choose Filter +
2: Choose Filter -
9: Start Rotor Readings
Notes: Use only in Service Mode
after a Base Line Test.
28
Rotor Read
1: Choose Filter +
2: Choose Filter -
Analyzer Memory Dump
G: Memory Dump
Notes: Use only after a Worklist in
Stand By.
This tests dumps all the preparations
parameters received and the photometric
readings.
DDC112/Photometry
D: Choose Mode
- DDC112 internal test mode
- DDC112 Photometry Mode
- Stop
+: Integration Time +0.5ms
-: Integration Time -0.5ms
Notes: Only works in Service Mode
This tests performs continuous
readings with the DDC112.
Remember stop the test for
continue working.
A25 Auto-Check
J: Last results
Caution: Dont’t abuse of this functions while the
analizer is running.
System Monitor
O: Power Supply Monitor
I: Reagent Refrigerator Monitor
W: EV&PUMP Diagnostic
[A25V2]
There are a few keys that work with the Hyperterminal, to press some keys the analyser give information about some
element, the following keys has the function:
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33
Service manual
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35
Service manual
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38
2.11 Schematic liquid circuit
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Service manual
3. SERVICE PROGRAM
The service program is used for the adjustment, checking and maintenance of the different components of the
analyzer. It is not supplied with the instrument, it is supplied to authorised technical services only. The personal
of the Technical Service must install it on the user’s computer in order to carry out the service requirements.
Once the tasks have finalised, the program must be uninstalled. To install the program, follow the instructions
on the installation CD ROM called Service. The original password for using this program is A25. The password
can be changed from the service program itself. If the service personnel forget the password, the original pas-
sword can be reinstalled by deleting the hidden file code.a25 from the application directory and relaunching
the program. Once the password has been introduced, the analyzer serial number is given and the name of
the operator is requested (by default Operator1). Press the Accept button and the main program appears. The
different functions of the service program are classified in the following categories:
- Adjustments: These make it possible to make different parameter adjustments required for the correct
functioning of the analyzer.
- Tests: Tests for checking the functionality of the analyzer.
- Utilities: Different technical utilities, such as, for example, washing or priming the dispensing system
or changing an optical filter.
- Registers:This enables the management of past adjustments, tests, incidences, repairs and maintenance
of the instrument.
- Monitor:These enable the low level communication with the analyzer to load new versions of the program
in the flash memory of the analyzer (firmware) or to consult the internal parameters of the instrument.
An emergency stop button (STOP on a red background) will be accessible at all times, and when pressed, it
switches off the analyzer and closes the application quickly.
To initialise the analyser in service mode, first launch the A25 Service application. The program first of all re-
quests a user or technician ID to be used in the program. Depending on the type of user identified, access to
the different parts of the program will be allowed or denied. The following screen appears:
Once the user has been identified correctly, the service program starts to initialise the analyser.
This screen appears when the analyser has finished the previous operations done to enter the SERVICE
40
mode. If the complete hardware of the analyzer is in correct conditions, the result “Hardware initiated correctly»
displays.
If any hardware element presents an operational problem, it will appear “Hardware not initiated completely” in
the screen, and the element that is not working correctly will be shown.
In order to close the screen and continue working, you should press the Accept button.
In order to get a printed copy of this initialization report, you should press the Print button.
NOTE: If an error has been reported and the technician continues working with the service program, he must
3.2. ADJUSTMENTS
These make it possible to make different parameter adjustments required for the correct functioning of the
analyzer. All the values to be adjusted have certain limited ranges, indicated by the service program. These
values are also given in an appendix at the back of this manual. If, after varying any of the parameters within
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Service manual
its permitted range, the analyzer is not tuned up, it indicates that the corresponding system is broken and in
need of repair.
This screen adjusts the needle thermostatation in such a way that the dispensing temperature of the reactions
is as close as possible to 37ºC. To make this adjustment, the analyzer must be initialised. The liquid to be dis-
pensed is taken from the system liquid container or from the bottle of reagent selected by the technician. The
technician must measure the temperature of the dispensed liquid with a thermometer calibrated at 37ºC. The
program shows the control set point temperature, which is the parameter that must be adjusted for the dispen-
sing temperature to be correct. This parameter must be different from 37ºC. When the technician so indicates,
the analyzer dispenses thermostated distilled water on a certain position in the racks tray shown on the screen.
The technician must measure the temperature of the water with the calibrated thermometer and introduce the
temperature on the screen. The analyzer automatically modifies the set point temperature in accord with the
temperature measured with the thermometer for the dispensing temperature to be 37ºC. The technician can
modify this set point temperature proposed by the program. On pressing Adjust, the analyzer thermostates
the needle with the new set point and, when the technician so requests, performs new dispensing operations.
Each time the set point temperature is modified, wait 1 minute before performing new dispensing operations
for the needle temperature to become stabilised. The technician must repeat this process until the dispensing
42
temperature is as near as possible to 37ºC. Pressing the Store button, the analyzer stores the current value
of the adjusted set point temperature. Pressing the Cancel button keeps the last stored value and the current
value is not stored. Pressing the Restore button restores the initial screen input value.
This screen makes it possible to adjust the thermostation system of the rotor in such a way that the reactions
temperature is 37ºC. To make this adjustment, place a well rotor in position and ensure that the analyzer has
been initialised. The rotor can be automatically filled with distilled water by pressing the corresponding button.
Once filled, the technician must wait a few minutes for the rotor to be thermostated. The temperature in the
rotor wells must be measured with a temperature calibrated at 37ºC through the dispensing hole of the rotor
cover. A button makes it possible to turn the rotor in increases of 15 wells to change the well on which the
measurement is being taken. The program shows the control set point temperature, which is the parameter that
must be adjusted for the temperature of the rotor to be correct. This parameter must be other than 37ºC. The
technician must measure the temperature of the water with the calibrated thermometer in the wells and enter
the temperature on the screen. The analyzer automatically modifies the set point temperature in accord with the
temperature measured with the thermometer for the rotor reactions temperature to be 37ºC. The technician can
modify this set point temperature proposed by the program. On pressing Adjust, the analyzer thermostates the
rotor with the new set point. Each time the set point temperature is modified, wait 5 minutes before performing
new dispensing operations for the rotor temperature to become stabilised. The technician must repeat this pro-
cess until the rotor temperature is as near as possible to 37ºC. Pressing the Store button, the analyzer stores
the current value of the adjusted set point temperature. Pressing the Cancel button keeps the last stored value
and the current value is not stored. Pressing the Restore button restores the initial screen input value.
This screen makes it possible to adjust the horizontal positioning (X, Y) of the arm. The arm housing must be
removed to see the position of the needle. Before making the adjustments, visually check the verticality of the
needle. If necessary, carefully straighten it up ensuring you do not damage it. On the screen, select the point
at which you wish to adjust the horizontal positioning. On pressing the Adjust button the arm initialises and
positions itself over said point. The technician has buttons to move the arm step by step over the horizontal
plain (X, Y) and vertically (Z). The arm can also be moved introducing a certain number of absolute movement
steps. These absolute movements of the arm must be made with the needle at its highest position so as not to
damage it (coordinate 0). The technician must lower the needle to the adjustment point and adjust its horizontal
position. When the position is satisfactory, save the current coordinates (X, Y) by pressing the Store button.
Pressing the Cancel button keeps the last adjustment values stored. Pressing the Restore button restores the
initial screen input values. At all times, the screen shows the current coordinates of the arm for the selected
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Service manual
point, the last coordinates stored and the initial screen input coordinates, as additional information for the te-
chnician. The technician may repeat the procedure to adjust the positioning of the arm at the different possible
adjustment points. These points are as follows:
(6) Zmax (on tray reference) Screw that fastens the wash station cover.
If you select the point of origin, automatic adjustment is possible in this position by pressing an AutoAdjustment
button (the process can take around 3 minutes).
This adjustment prevents the needle from colliding with the rack tray.
44
Adjusts the needle to the central position screw (same position as the adjustment of the rack tray reference).
This adjustment consists of moving the needle until it comes into contact with the reference screw on the rack
tray. This operation can be completed by moving the needle to an absolute Z coordinate, Z-direct box, and then
using the needle up and needle down keys until contact is made with the screw. The programme then calculates
the real reference by adding a constant value so that the needle is lowered to the rack position. Bear in mind
that the encoder detection of the needle is not enabled. Consequently, if there is a collision by entering a high
value in the Z-direct box, it may collide with the screw. In this case, use the Home button to reinitialise the Z
axis and repeat the process from the beginning.
This it is an optional adjustment. It is used to reduce the dead volume (without colliding with racks), adding or
subtracting some steps to the value fit as Zmax.
Steps to follow:
1. Select the type of Rack
2. Place a rack of the selected type at Position 1 in the rack tray.
3. Place two cups, tubes or bottles in the extreme positions of rack: Positions 1 and 12 for Samples; Positions
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Service manual
NOTE: During this adjustment, the probe detection of encoder is not activated. Therefore, if it collides (due to
inserting a too-high value), the autoelevation system may act. In this case, you should press the Home Z button
to restart the Z axis and repeat the process from the starting point.
This screen enables the adjustment of the positioning of the rotor with regard to the dispensing point and the
optical system. One or the other is selected by means of two different tabs.
The analyzer initialises the rotor and positions the first rotor well at the currently programd dispensing position.
The technician has buttons to move the rotor step by step to adjust, if necessary, this position and buttons for
finer adjustment of the X coordinate over the dispensing point. At all times, the screen shows the current dis-
pensing coordinate on the first well and of the X axis position, the last coordinate stored and the initial screen
input coordinate, as additional information for the technician. When this is satisfactory, the current coordinate
of the dispensing point of the first well can be stored by pressing the Store button. Pressing the Cancel button
keeps the last stored value and the current value is not stored. Pressing the Restore button restores the initial
screen input value.
46
3.2.4.2. Centering of the rotor with regard to the optical system
This adjustment is necessary only if the Rotor Centering Adjustment has been carried out with regard to the
dispensing point (3.1.4.1.). This adjustment must be made with the rotor cover in position. The analyzer initialises
the rotor and fills the first 3 wells of the rotor with distilled water. Next, step-by-step optical readings are made
through these wells at the wavelength selected by the technician. Once the readings have ended, the program
shows a graph of the light intensity measured on the rotor steps. On this graph, the program indicates at which
points the optical readings are made on each of the 3 wells when the analysis is made, with the coordinate of
the reading point of the first well currently programd in the analyzer. If necessary, the technician can move the
reading points over the graph jointly using two buttons. The optimum reading point is that which globally maxi-
mises the light intensity for the three wells. At all times, the screen shows the current coordinate of the reading
in the first well and the last coordinate stored, as additional information for the technician. When the position
is satisfactory, the current coordinate of the reading point of the first well can be stored by pressing the Store
button. Pressing the Cancel button keeps the last stored value and the current value is not stored.
This adjustment must be made with the rotor cover in position. The analyzer initialises the rotor and the filter
wheel and fills the first rotor well with distilled water. Next, it takes optical readings through this well, turning the
filter wheel step by step, with a certain integration time as indicated by the technician (the concept of integration
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Service manual
time is explained in the section on photometric adjustments). Once the readings have ended, the program shows
a graph of the light intensity measured on the steps of the filter wheel. On this graph, the program indicates at
which points each of the filters is positioned when optical readings are taken when the analysis is carried out,
with the coordinate of the positioning of the filter 0 currently programd in the analyzer. If necessary, the technician
can move the reading points over the graph jointly using two buttons. The optimum reading point is that which
globally maximises the light intensity for all the filters. At all times, the screen shows the current coordinate
of the filter 0 and the last coordinate stored, as additional information for the technician. When the position is
satisfactory, the current coordinate of the positioning of the filter 0 can be stored by pressing the Store button.
Pressing the Cancel button keeps the last stored value and the current value is not stored.
This screen makes it possible to set the level control scales with the empty waste and distilled water containers
(0% capacity) and when they are full (100% capacity). The maximum capacity of the containers is approximately
3L. The technician must choose whether he wishes to set the distilled water or waste container scales, with the
corresponding container full or empty. According to the requested adjustment, the corresponding container, full
or empty, must be placed in position and the Adjust button pressed. Based on the settings made, the analyzer
automatically adjusts the scales. On pressing the Store button, the analyzer saves the new values of the adjusted
parameters. Pressing the Cancel button keeps the last stored values and the current values are not stored.
This screen allows fitting the sensitivity of the capacity level detection system of the probe. The pediatric rack
is a rack with 15mm diameter with the adapter tubes and the pediatric wells inserted.
In order to make the adjustment, first of all you have to select a rack and place on it the following configuration
of racks and samples:
• Paediatric Rack: place 4 paediatric glasses in positions 5, 6, 17 and 18 with 400 uL of liquid system.
• Rack of 13/15mm sample: place 4 tubes in positions 5, 6, 17 and 18 with 400 uL of liquid system.
• Rack of reagents: place 2 bottles of 20mL in positions 5 and 6 with 2mL of liquid system.
• Fridge: Place 2 bottles of 20mL in the position 5 and 6 of the center with 2mL of liquid system.
It is possible to see a graphic with the position of the tubes and racks in the screen photo. When pressing the
Adjust button, the arm takes some sensitivity readings automatically until detecting the water in each one of
the tubes. Once this operation is finished, the arm is parked in its original position and it shows the sensitivity
results of each one of the tubes. Once these readings are finished, the program calculates the average of all
sensitivities; the result is the average sensitivity. The technician should notice that the sensitivity values of each
48
tube have to be similar; otherwise, he should repeat the whole measurement.
Repeat this adjustment per each rack type.
The sensitivity value can also be introduced manually for each rack in the corresponding box.
Pressing the button Save, the analyser saves the new adjusted sensitivity value. Pressing the button Close,
the old value stays. Pressing the button Restore, the initial value of entry to the screen is restored. A manual
sensitivity value can be inserted in the corresponding box.
3.3. TESTS
Various tests make it possible to check that the different components of the analyzer function correctly.
Through these tests, the technician can check the correct functioning of all the analyzer motors step by step.
The screen makes it possible to choose the motor to be tested and the test that is to be carried out. The analyzer
uses the following motors step by step:
- X axis of the operating arm.
- Y axis of the operating arm.
- Z axis of the operating arm.
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Service manual
- Dispensing pump
- Rotor
- Filter wheel
All the motor tests can be performed without the covers and housing of the analyzer. After the verifications,
the operating arm always returns to its resting position. To test the motor of the dispensing pump, the arm is
positioned over the washing station. It is convenient for the dispensing system to be primed so that the piston
does not function dry. The following is a description of the different tests that can be performed.
This test displaces any of the mobile components to the desired point along its range of functioning, introducing
the corresponding absolute coordinate or moving it step by step. The speed and acceleration of the movement
are those used in the normal functioning of the analyzer.
50
3.3.1.3. Loss step test
This test makes it possible to check if a motor misses steps when performing a certain sequence of movements.
The test can be carried out with the speed and acceleration used in the normal functioning of the analyzer or
with these magnitudes increased by 10% to check the functioning safety margin.
This test makes it possible for a certain sequence of movements to be performed continually. The technician
can program the duration of the test, which can be cancelled at any moment. Depending on the motor selected,
there is a minimum stress mode time (but in no case is it higher than 50 seconds).
The Z axis of the operating arm has an encoder to detect if there have been missed steps as a result of a co-
llision with the needle. In the case of a power failure, a mechanical system automatically raises the needle. On
selecting the corresponding options, the analyzer checks the functioning of each of these devices.
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Service manual
This test checks that the needle does not collide with the bottles on the rack tray. Select the rack type (reagent,
paediatric, 30 mm or 15 mm), the position of the rack on the tray and the position of the bottle or well on the
rack.
Press the Start button to move to the selected position and check if the needle collides with the bottle or well
or if there is space between the needle and the bottle.
Repeat the process in the positions required by the user.
The analyzer uses three 3-way electrovalve to manage the dispensing operations. The washing system of the
needle uses a 2-way electrovalve and two diaphragm pumps. The screen makes it possible to choose the device
to be tested and the test that is to be carried out. The devices that can be tested independently are:
52
To carry out these tests, the dispensing system should be primed. The following is a description of the different
tests that can be performed.
This test makes it possible for a certain sequence of device switching to be performed continually. The technician
can program the duration of the test, which can be cancelled at any moment.
This test makes it possible to check the functioning of the needle self-centering system. During its initialisation,
the analyzer uses this system to check the presence of the needle and its verticality and automatically correct
small deviations. The test consists of simply running this process. The technician can remove the housing of
the arm to observe the test. On the finalisation of the test, the program shows the deviation (x, y) found in the
motor steps.
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Service manual
This test checks the functioning of the system for detecting the capacity of the needle in bottles of reagent and
sample tubes.
This test checks the functioning of the system for detecting the capacity of the needle in reagent bottles and
sample wells. The test can be performed in any position on the tray.
First select the rack type, then the position of the rack on the tray and, finally, the position of the bottle/well on
the rack. Press the Test button and the program will move the arm to the indicated position and check whether
or not liquid is detected, depending on whether the bottle is full or empty.
Repeat the test as many times as the user considers necessary.
This screen makes it possible to check that the dispensing temperature of the reactions is around 37ºC. To make
this adjustment, the analyzer must be initialised. The technician must measure the temperature of the dispen-
sed liquid with a thermometer calibrated at 37ºC. The program shows the set point temperature of the current
control. This parameter must be different from 37ºC. When the technician so indicates, the analyzer dispenses
thermostated distilled water on a certain position in the racks tray shown on the screen. The technician must
measure the temperature of the water with the calibrated thermometer and introduce the temperature on the
54
screen. The program indicates if the temperature measured is within the tolerated error margins and stores this
value for the test result reports. The liquid to be dispensed is taken from the system liquid container or from the
bottle of reagent selected by the technician.
This screen makes it possible to check that the temperature of the rotor reactions is 37ºC. To make this test, the
analyzer must be initialised. The methacrylate rotor can be automatically filled with distilled water by pressing the
corresponding button. Once filled, the technician must wait a few minutes for the rotor to be thermostated. The
temperature in the rotor wells must be measured with a temperature calibrated at 37ºC through the dispensing
hole of the rotor cover. A button makes it possible to turn the rotor in increases of 15 wells to change the well
on which the measurement is being taken. The program shows the set point temperature of the current con-
trol. This parameter must be other than 37ºC. The technician must measure the temperature of the water with
the calibrated thermometer in the wells and enter the temperature on the screen. The program indicates if the
temperature measured is within the tolerated error margins and stores this value for the test result reports.
This screen contains a set of tests to check the functioning of the optical system. The tests are classified under
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Service manual
different tabs. First of all, the base line and darkness count tests must be made in order to be able to carry out
the remaining tests. To perform these tests, the analyzer must be initialised.
The optical system has a photodiode that generates an electrical current proportionate to the light intensity on
it. time. An AD converter converts the accumulated load into a digital value called count number, between 0
and 1048576. During normal functioning, the analyzer automatically adjusts the integration time for each filter
when the analysis begins and after initialisation. When the first photometry test is performed, the integration
times are also automatically adjusted. These times are adjusted in such a way that the count number of the
base line for each wavelength is as near as possible to 950000. In this way, the dynamic range of the detection
system is adapted to the light intensity present at each wavelength. The filter wheel has 10 positions. Position
0 must always contain a covered filter so that the analyzer can perform the darkness adjustment. Positions 1
to 9 can be used for optical filters.
When this test is run for the first time, the analyzer fills the first 3 rotor wells with distilled water. The analyzer
automatically adjusts the integration times and makes a base line with each of the available filters in each of
the 3 wells. The program shows the current integration times for each of the filters and the average for the 3
wells of the count numbers obtained with each filter. The screen shows the corresponding alarms in the case
of anomaly. It is also possible to access a screen where it is possible to manually vary the integration times
56
to check their effect on the count numbers. And another screen where it is possible to assign calculated in-
tegration times as reference integration times for each filter. This screen is recommended when a filter or the
lamp is physically changed. After performing the test, the analyzer continues to take optical readings using the
automatically adjusted integration times.
The program shows the current integration times for each filter. On running the test, the analyzer positions the
covered filter and measures the darkness counts with each of the integration times. Each time an optical reading
is taken, the analyzer subtracts these darkness counts from the count numbers measured to obtain the light
intensity. The program shows the values obtained and issues the corresponding alarms in case of anomaly.
The values should be around 4100 - 4300. All the count numbers shown by the tests given as follows have the
darkness counts subtracted.
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Service manual
To perform this and the following tests, the base line and darkness count test must have first of all been perfor-
med. This test takes absorbance readings during 1 minute with the filter wheel in fixed position. The technician
can choose the rotor well on which he wishes to take the readings and fill it with the liquid he desires. He can
choose which wavelength he wishes to use. The test can also be performed with the filter covered. When the
readings end, the screen graphically displays the count numbers obtained and the absorbances with regard to
the corresponding base lines. The program also shows the averages and/or standard deviations of the count
numbers and the absorbances.
3.3.7.4. Stability
This test takes absorbance readings during 30 minute with the filter wheel in fixed position. The technician
can choose the rotor well on which he wishes to take the readings and fill it with the liquid he desires. He can
choose which wavelength he wishes to use. The test can also be performed with the filter covered. The test can
be cancelled at any time. When the readings end, the screen graphically displays the count numbers obtained
and the absorbances with regard to the corresponding base lines. The program also shows the averages and/
or standard deviations of the count numbers and the absorbances.
58
3.3.7.5. Repeatability moving filter wheel
This test takes absorbance readings during 10 minute moving the filter wheel randomly. The technician can
choose the rotor well on which he wishes to take the readings and fill it with the liquid he desires. The test can
be cancelled at any time. When the readings end, the screen graphically displays the count numbers obtained
and the absorbances for each filter with regard to the corresponding base lines. The program also shows the
averages and/or standard deviations of the count numbers and the absorbances for each filter.
This test enables individual absorbance readings. The technician can choose the rotor well on which he wishes
to take the readings and fill it with the liquid he desires. He can choose which wavelength he wishes to use.
The screen shows the count number obtained, the absorbance with regard to the corresponding base line, the
value of the base line.
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Service manual
The user can use this test to check the optical status of a reactions rotor. He or she can choose the optical filter
with which the test is to be performed. The technician must place the rotor in the analyzer and press the Test
button. If the Automatic Fill option has been chosen, the analyzer fills the 120 rotor wells with distilled water
and then makes a base line on each well with the chosen filter. The analyzer graphically displays the absorban-
ces related to the average of all the wells and tells the technician the state of the rotor (optimal, adequate or
unusable). After the test, the user must remove the rotor of the analyzer, empty it and dry it completely before
using it for analyses.
This screen makes it possible to check the functioning of the level control scales of the waste, distilled water
and the washing solution extern bottle containers. On pressing the Test button, the screen shows the level of
liquid measured by the analyzer. The external bottle container only indicates full (100%) or empty (0%).
60
3.3.9. Racks and covers detection test
This test makes it possible to check the functioning of the different detectors incorporated in the analyzer.
- Open detector of the general cover of the analyzer.
- Rotor cover presence detector.
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Service manual
The technician can manipulate the corresponding components, for example, open and close the cover of the
analyzer and the screen shows the state of the detectors in each case.
On pressing the Test button, the computer attempts to establish communication with the analyzer. The program
tells the technician if it has been possible or not.
The technician can select Automatic Configuration or Manual Configuration. In the case of the latter, he can
define the Port and the Speed.
This test makes it possible to continually reproduce work cycles of the analyzer similar to those made during
the preparation and reading of reactions in a normal working routine, but dispensing at the washing station
instead of the rotor. It is necessary for the dispensing system to be primed so that the piston does not function
dry. All the racks must be removed from the racks tray. This test can be made without the covers and housing
of the analyzer. The technician can program the number of cycles he wishes (1 cycle = 15 seconds). The test
can be cancelled at any time.
Once the test has been launched, the screen provides regular information about the current status of the pro-
cess. If an error occurs during the process, the test ends and the screen displays a message indicating the
element causing the error.
Partial stressing of the elements of the analyser is possible. The following elements can be stressed partially:
• X axis
• Y axis
• Z axis
• Reactions rotor
• Filter wheel
• Dispensation pump
• Membrane pumps
• 2-way electrvalve
• 3-way electrovalve
62
3.4. UTILITIES
The program contains various technical utilities. These utilities are also accessible from the user program.
On clicking on the Disassemble Needle button, the operating arm positions itself over the rack tray. The program
alerts the technician to remove any object positioned under the arm. On clicking OK, the needle descends
and the technician can remove it to work with it or change it. To remove the needle, unscrew it by holding the
top fitting. If, while handling the needle, the carriage rises due to the pressure made by the technician, press
the Lower Needle button for the needle to descend once again. Once the needle has been reassembled on
the analyzer, press the Park button for the needle to rise. It performs the self-centering test and the arm finally
returns to its parked position. These operations must be done with utmost care since they are carried out with
the analyzer cover open and the needle may be contaminated. Laboratory gloves must always be used.
On pressing the Test button, the analyzer fills the conduits of the dispensing system and the washing station
with distilled water. To perform this operation, the operating arm is moved to the washing station. The technician
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Service manual
can choose whether he wishes to prime the dispensing system, the washing system or both. The technician
can choose which liquid want to prime the dosification system, with air, with washing solution or with liquid
system.
On pressing the Wash button, the analyzer washes the dispensing system internally and externally. To perform
this operation, the operating arm is moved to the washing station. The technician can choose between perfor-
ming the wash with distilled water or wash solution. In the case of the latter, the analyzer asks the technician
to place a bottle of wash solution in stead of the distilled water container or to fill the latter with wash solution.
Once the wash has been performed, the analyzer asks for the distilled water container to be put back in position.
Finally, the analyzer primes the system with distilled water.
When entering the screen, it is possible to choose between: Changing or checking the lamp. When a new
lamp is installed, this utility must be used to notify the analyzer that the lamp has been changed and optimize
the luminosity of the photometric system. The lamp must be changed with the analyzer in sleeping mode. If
the analyzer is on standby mode, the program shuts it down automatically. The lamp must never be touched
with fingers. Once the new lamp has been installed and the covers of the optic and rotor put back, access the
64
change lamp utility and press the Test button. The program starts up the analyzer, checks the light intensity of
the optical system, shuts down the analyzer and then requests the technician to remove the lamp holder again
and replace it again turning it 180º on the axis of the lamp. If the temperature of the lamp holder is high, wait until
it cools down or use pincers to hold it. The program starts up the analyzer again, measures the light intensity
of the optical system again, compares the light intensity in both possible positions and chooses the greatest
luminosity. If it is the current position, it tells the technician that the test is complete. If the best position were
the previous one, the program shuts down the analyzer and asks the technician to remove the lamp holder and
replace it, turning it 180º on the axis of the lamp, returning the lamp to its initial position. If the option selected
at the beginning was to Check the Lamp, the process is the same but without shutting down the analyzer at
the beginning.
This screen enables the modification of the analyzer filter wheel. The wheel has 10 positions. Position 0 must
always contain a covered filter so that the analyzer can perform the darkness adjustment. Positions 1 to 9 can
be used for optical filters. All the positions of the wheel must be occupied for it to work correctly. The positions
that do not contain an optical filter must be occupied by a covered filter. The analyzer includes as standard 8
optical filters in positions 1 to 8 and two covered filters in positions 0 to 9. If one of the filters is to be changed,
select the desired position of the wheel and press the Change Filter button. The analyzer automatically posi-
tions the filter wheel appropriately so that the technician can change the filter through the window of the optical
system. Next, if it is different, introduce the wavelength of the new filter that has been installed. If the filter is
covered, introduce value 0. On closing the screen, the analyzer asks if the filters have actually been physically
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Service manual
changed and a series of warnings are given to the technician telling him he must bear in mind whether or not
he has changed a filter.
On pressing the Start button, the analyzer activates some of its mobile components, imitating functioning during
a work routine. The activated mechanical components are the operating arm, the reactions rotor and the filter
wheel. On pressing the Cancel button, the analyzer finishes the current cycle and returns to its rest position.
From this screen, it is possible to read the current adjustments that the analyser is using by pressing the button
Read Adjustments.
It is allowed to save these adjustments in a file. The technician selects the name and location of this file.
Also from this same screen and with the button Load Adjustments, the technician is allowed to select an ad-
justment file and to load it in the analyzer. Once the adjustment loading is made, the analyser turns off and the
application is closed. When reinitiating the application, the new loaded adjustments will be already active.
From the firmware version 2.80, the programme counts the number of cycles of each element and the task of
the analyser. From this menu, it is possible to read the cycles completed by the analyser. The screen displays
the said cycles with the corresponding units.
66
The programme automatically saves a copy of the adjustments and cycles read in a file. This file is located in
the following folder:
When a physical element of the analyser has to be changed, e.g. the Z axis belt, the counter must be reset
to zero for it to correspond to the number of cycles actually stored in the analyser. To perform this operation,
select the box of the element that is to be initialised and enter the number of cycles in the enabled box. Then
press the Load cycles button.
Using the Load adjustments button, this screen also enables the technician to select an adjustments file and load
it in the analyser. When the adjustments are loaded, the cycles are also loaded. Perform this operation when
a CPU board has to be changed. This avoids having to completely readjust the analyser; only the following
sections will have to be readjusted:
Scales
Level detection sensitivity
Needle thermostatation
Rotor thermostatation
In this screen the type of rotor is introduced. Each rotor comes labelled with a letter in its top part. Select in
this screen the type of rotor to use. For rotors marked with A letter, only select the letter. For the rotors marked
with other letters, select OTHERS and then introduce the light path that will come it within the box of rotors or
of the distributor.
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Service manual
3.5. REGISTER
This enables the management of past adjustments, tests, incidences, repairs and maintenance of the instru-
ment.
The technician can enter the analyzer serial number so that it appears on printed service reports. If an ente-
red serial number is changed, the service records are reinitiated. In this case, the technician can store all the
previous data in a file.
The technician can enter his name so that it appears on the printed service reports.
The program can display and print various service reports. The printed reports contain the analyzer serial
number and the name of the current technician.
Reports are stored organised by: Adjustments, Tests, Utilities, Monitor and Summary of actions and tasks
carried out.
In all cases, it is possible to select the actions carried out within a range of dates chosen by the technician.
The technician can enter short descriptions of the incidences that may happen in the analyzer and the repairs
and maintenance operations that may be performed to the instrument in the Observations box.
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3.5.3. Language change
This makes it possible to choose the language used in the service program.
3.5.4. Users
· SAT. This user has full access to the programme. This user has permission to create and/or delete
other users.
· User. This user has restricted access to the programme. This user can only perform the tests and run
the utilities. He/she can not make any adjustments or load any previously saved adjustments files or change
the firmware version of the analyser.
From the Users menu, it is possible to create, delete and change users. The Change password option is for
each user to change his own password.
3.6. MONITOR
These enable the low level communication with the analyzer to load new versions of the program in the flash
memory of the analyzer (firmware) or to restore the default adjustment parameters.
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Service manual
The firmware of the analyzer resides in a permanent flash memory. The change of this program can be made
through the computer without the need for changing the memory chip.
To load the new version, press the Start button, previously indicating where the program is located using the
Open button.
From version 3.0.2, before loading a new firmware, the program checks that the file is correct. If is so, the
program sends it directly to the analyzer. If there is an error message, please contact the Technical Service to
replace/recover the corrupt file
First of all, the current content of the flash memory is deleted and then the new program is loaded. This ope-
ration may take several minutes.
Once the program has changed, the analyzer is restarted with the new version of the program. While the copy
process is being performed, the screen indicates the percentage completed.
There is also the option to Restore Default Adjustments, selecting the option and pressing Start.
If the technician wants the analyzer to enter monitor mode (e.g. because the analyzer does not respond be-
cause the firmware was incorrectly loaded, he may do so by shutting down the analyzer, pressing the Force
Monitor button and then rebooting.
Once the new programme has been loaded or the default adjustments have been restored, exit the monitor by
pressing the Close button.
In this section, the service options in the user program will be described. These options are intended to confi-
gure the user’s access level.
Each section explains how to manage and create different levels of access to the user program of the analy-
ser.
When the program is installed for the first time, there is not a created user and access to the program is com-
plete.
To activate the option of level of access to the analyser, the first time you should enter as administrator, whose
values are:
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delete results or alarms. This user has total access to the rack and profile programming and to the analyser’s
configuration (except for changes of filters). He can change his own password.
• Supervisor, is the user with a medium access level. This user has got the same privileges as the operator
user’s and, in addition, he has got permissions to modify the programming of techniques in the calibration
parameters and the control values. He can create a restricted number of new techniques, that is defined at
the moment of creating such user and that it is a default setting of 5. He can also modify the programming of
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Service manual
contaminations and change the analyser’s filters. He can change his own password.
• Administrator, is the user with total access to the analyser’s functions. He can create new users -as much at
supervisor as at operator level-, eliminate or modify users. When creating supervisor users, he has to indicate
the maximum number of new techniques that can create. He can activate or deactivate Work Without Passwords
(option within the Configuration menu). He can also activate/deactivate the working without cover detection
(this option is useful for the technical service to make verifications without needing to let the cover down). This
option activates solely when the passwords are active.
When users are created, the access is limited to different parts of the program. When starting the program, an
identification of the user is requested, by the user name and a password, and then the program will automatically
restrict the different parts of the program depending on the access level permitted.
Whenever you want, you can change the user by means of the option Change of user from the User menu.
It is also allowed to eliminate users already created. Each user is capable of changing his password. All these
options can be reached from the user menu.
In order to access the consumption of reagents, it is first necessary to configure the program with the option
of working with passwords. The administrator user is the only one that can access this menu -this option is
deactivated for any other user.
In order to generate a list of the consumption of reagents, the administrator has to introduce the dates between
which he wants to know the consumption. For this, it appears a screen like this:
Such option creates two files of results, one in text format .txt and the another one in excel format .xls. These
files will be located at directory within the application directory, it will usually be:
c:\Archivos de programa\A25\Reagents
72
4. RECOMMENDATION
Clean the outside of the end with 70º alcohol, use the utility of end change to access it.
It is recommended that the following maintenance actions are performed annually or every 1,000 working hours.
Operating arm
1. Check the state and stress of the belts.
2. Replace the components in an unsatisfactory state.
3. Make the adjustments and tests related to the service program.
Dispensing system.
1. Exhaustively wash all the dispensing circuit with washing solution and rinse it with distilled water.
2. Remove and clean the needle. Check its state.
3. Check the state of all the dispensing circuit tubes. Check the absence of obstructions or diameter changes.
4. Check the leakproof quality of the fittings and the 3-channel electrovalve body set.
5. Check the leakproof quality of the dispensing pump fitting and seal. Check there is no loss and no bubbles.
6. Clean the distilled water container filters.
7. Clean the washing station.
8. Check the state of the washing system tubes.
9. Check the leakproof quality of the fittings and the 2-way electrovalve body.
10. Replace the components in an unsatisfactory state.
11. Make the adjustments and tests related to the service program.
Reactions rotor
1. Check the state of the heating channel.
2. Check the belt stress.
3. Replace the components in an unsatisfactory state.
4. Make the adjustments and tests related to the service program.
Optical system
1. Check the state of the lamp.
2. Clean the filters.
3. Clean the lenses.
4. Clean the photodiode.
5. Replace the components in an unsatisfactory state.
6. Make the adjustments and tests related to the service program.
a) Never use detergents or abrasive products for cleaning the surface of the analyzer. Use only a damp cloth with
water and pH-neutral soap.
b) If a reagent or corrosive product spills or splashes onto the apparatus, clean it with a damp cloth and soap imme-
diately. If necessary, protect your hands with appropriate laboratory gloves.
c) All the components of the analyzer have drainage conduits leading to the exterior to enable the elimination of any
liquid spilled and to prevent the apparatus from flooding. If the spillage is significant, the liquid spilled onto the table
through the drainage conduits and the analyzer must be adequately cleaned.
d) When not in use, close the main cover of the analyzer to protect it from dust.
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Service manual
The components of the optical system must be cleaned periodically in order to keep them free from dust and dirt.
These components are the lamp, the lenses, the filters and the photodiode. The recommended necessary material
is as follows:
- Special paper for cleaning optical components (non-abrasive paper which does not leave solid residue).
- Ether and alcohol solution at 50%
- Cotton wool buds.
- Small bellows.
All the optical components must be handled in an area of maximum cleanliness and with great care given that they are
fragile and delicate. To remove them and refit them, the corresponding instructions given in the Maintenance chapter
must be carefully followed. Avoid touching the useful area of these components with fingers. The lenses, filters and
the photodiode must be held by the sides. Do not touch the lamp bulb. To handle the lamp, use the wrapping, cutting
it at the terminal ends and squeezing them until they come out. To clean the optical components, bear in mind the
following:
a) Remove the dust on the surface of the component with the bellows. This will avoid scratching by particles when
cleaning with paper.
b) Carefully clean the surface of the component with the cleaning paper.
c) If the dirt is persistent or greasy, clean the component with the paper moistened with the alcohol and ether solution.
Then dry with dry paper. To clean the filters or the window of the photodiode, use the cotton wool buds with the
paper in difficult and delicate areas.
d) Finally, it is recommended that you use the bellows once again, thus removing any remains of paper or cotton.
The analyser automatically performs conditioning of the fluid system at warm-up and shut-down. This conditioning
consists of a 3 primings of the dosing system, the first with air, the second with washing solution and the third and
last with system liquid. To perform this conditioning, the bottles of system liquid (marked with a blue dot) and washing
solution (marked with a green dot) must be full, and the waste bottle (marked with a red dot) must be empty. The final
conditioning consists of performing a priming with washing solution.
With initial wash, the system is ready to work in optimum conditions for the whole workday, offering its best performance.
With the final wash, the analyser cleans the tip at the end of the day’s work, thus keeping it in perfect condition for the
next workdays. The user can also perform conditionings of the dosing system when desired by pressing the conditioning
button in the vertical button bar on the monitor screen while the analyser is in standby mode. It is also recommended
to clean and check the status of the filters of the system liquid container at least once every 3 months.
74
If the needle is obstructed by solid residue, it must be removed and cleaned using the metal cleaning rod supplied
with the analyzer. For this, the Remove the dispensing needle utility should be used. It is also recommendable to
clean the outside surface of the needle with a piece of cotton or a soft cloth dampened with alcohol. The needle must
be replaced if it is noticeably deteriorated.
It is important for the interior of the instrument to be free from dust at all times in order to preserve the correct functio-
ning of the different components. For this, remove the front housing of the analyzer and carefully clean the dust inside
the instrument.
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Service manual
A I. TECHNICAL SPECIFICATIONS
PLEASE NOTE
The manufacturer accepts no liability for damage caused by incorrect use of the apparatus.
GENERAL SPECIFICATIONS
Automatic random and continual access analyser aimed at giving results per patient, with direct photometric reading
over a reactions rotor.
3 0 72 30 refrigerated
REAGENT REFRIGERATION
DISPENSING SYSTEM
NEEDLE
Detachable tip
Vertical length 110 mm
Capacity level detection
Self-adjustment of position
A system of valves that enables the fluid dispensing system to be filled with system liquid, air or washing solution.
3L bottle of washing solution external to instrument equipped with minimum level detector.
Connections of bottle of washing solution with quick antidrip plug for liquid and jack connector for signal level. Both
on the back panel of the instrument.
WELL ROTOR
Semi-disposable extractible methacrylate rotor
Number of wells 120
Accepted reaction volumes 200 µL - 800 µL
Light path length 6 mm
OPTICAL SYSTEM
halogen lamp 12 V, 20 W
Wavelength selection with compensated interferential filters
Detection system with silicon photodiode and 20-bit AD integrator-converter
Measurement range from -0.05 A to 2.5 A
Reading speed 5 readings/s
Maximum number of filters 9
Base configuration of the filter wheel 340, 405, 505, 535, 560, 600, 635, 670 nm
Wavelength precision ± 2 nm
Bandwidth 10 ± 2 nm
Digital resolution < 0.0001 A
Base line stability max. 0.004 A in 30 mins, at 505 nm
Repeatability of the reading system ± 0.0005 A a 0.1 A (CV = 0.5%)
(1 SD, 505 nm, with filter movement) ± 0.003 A a 1.0 A (CV = 0.3 %)
± 0.005 A a 2.5 A (CV = 0.2 %)
Optical repeatability between wells ± 0.003 A a 340 nm
± 0.002 A a 505 nm, 670 nm
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Service manual
POWER REQUIREMENTS
ENVIRONMENT CONDITIONS
Interior use
Altitude < 2500 m
Temperature 10°C - 35°C
Relative humidity <75%
Contamination level 2
78
• UNE-EN 55014:2002+A1:2002+A2:2004 “Electromagnetic compatibility. Requirements for household appliances,
electric tools and similar apparatus. Emission”
• UNE-EN 61000-3-2:2006 “Electromagnetic compatibility (EMC) – Part 3: Limits –Section 2: Limits for harmonic
current emissions”.
• UNE-EN 61000-3-3:1997+A1:2002+A2:2006 “Electromagnetic compatibility (EMC) – Part 3: Limits –Section 3:
Limitation of voltage fluctuations and flicker in low-voltage supply systems”.
• UNE-EN 61000-4-2:1997+A1:1999)+A2:2001 “Electromagnetic compatibility (EMC) – Part 4: Testing and measu-
rement techniques – Section 2: Electrostatic discharge immunity test”.
• UNE-EN 61000-4-3:2003+ERR:2003+A1:2004 “Electromagnetic compatibility (EMC) – Part 4: Testing and mea-
surement techniques – Section 3: Radiated, radio-frequency, electromagnetic field immunity test”.
• UNE-EN 61000-4-4:2005 “Electromagnetic compatibility (EMC) – Part 4: Testing and measurement techniques –
Section 4: Electrical fast transient/burst immunity test”.
• UNE-EN 61000-4-5:1997+A1:2001 “Electromagnetic compatibility (EMC) – Part 4: Testing and measurement
techniques – Section 5: Surge immunity test”.
• UNE-EN 61000-4-6:1998+A1:2001 “Electromagnetic compatibility (EMC) – Part 4: Testing and measurement
techniques – Section 6: Immunity to conducted disturbances, induced by radio-frequency fields”.
• UNE-EN 61000-4-11:2005 “Electromagnetic compatibility (EMC) – Part 4: Testing and measurement techniques
– Section 11: Voltage dips, short interruptions and voltage variations immunity tests”.
• UNE-EN 61000-4-8:1996+A1:2001 “Electromagnetic compatibility (EMC). -Part 4: Testing and measurement te-
chniques. Section 8: Power frequency magnetic field immunity test.”.
• UNE EN 22233-1992 (ISO 2233-1986). Packaging — Complete, filled transport packages and unit loads — Con-
ditioning for testing
• UNE EN 24180-2-1992 (ISO 4180-1980). Complete, filled transport packages — General rules for the compilation
of performance test schedules.
• UNE EN 22247-1992 (ISO 2247-2000). Packaging — Complete, filled transport packages and unit loads — Vibra-
tion tests at fixed low frequency
• UNE EN 22248-1992 (ISO 2248-1985). Packaging — Complete, filled transport packages — Vertical impact test
by dropping
The manufacturer reserves the right to modify any technical specification without prior notice.
79
Service manual
80
A II. ADJUSTMENT MARGINS TABLES
Main voltage measurement points 13/15mm tube sample rack [45,100]
CIIM00007 Power supply board Reagent rack outside fridge [10,60]
TP1- 5V [4.9-5.1] V Reagent rack inside fridge [10,60]
TP2 - 12V [11.7 -11.9] V Positioning of XY fridge adjustment
TP3 - 12V [11.8-12.2] V X fridge [1700, 1730]
Y fridge [1285, 1315]
TP4 - 12V [11.8-12.2] V
TP5 - 12V [11.8-12.2] V Positioning of the dispensing point
Dispensing rotor [-10,30]
TP36 [32-40] V
Fine rotor X [2860,2897]
Positioning of rotor in optic
CIIM0006 Microprocessor board Positioning [-12,6]
TP50 - Vref Z motor [3-4] mV Filter wheel positioning
TP51 - Vref Ymotor [3-4] mV Positioning [-6,0]
TP52 - Vref Xmotor [3-4] mV
Needle thermostatation
TP53 - Vref pump motor [3-4] mV
Set point temperature [35.5,39.5]
TP54 - Vref filter wheel motor [3-4] mV
Rotor thermostatation
TP55 - Vref rotormotor [3-4] mV
Set point temperature [36.5,38]
Integration times
Main adjustment values of the analyzer F1 340 nm [30,70]
F2 405 nm [6,26]
Tolerances F3 505 nm [6,26]
Operating arm positioning F4 535 nm [6,26]
X axis loss of steps F5 560 nm [6,26]
Loss of steps [-3,3] F6 600 nm [6,26]
Y axis loss of steps F7 635 nm [6,26]
Loss of steps [-3,3] F8 670 nm [6,26]
Z axis loss of steps Number of counts
Loss of steps [-3,3] F1 340 nm [750000,960000]
Loss of steps rotor motor F2 405 nm [840000,940000]
Loss of steps [-3,3] F3 505 nm [840000,940000]
Loss of steps filter drum motor F8 670 nm [840000,940000]
Loss of steps [-3,3] Darkness counts
Loss of steps pump motor F1 340 nm [3700,4300]
Loss of steps [-4,4] F2 405 nm [3700,4300]
XY position adjustment F3 505 nm [3700,4300]
X origin [5,30] F8 670 nm [3700,4300]
Y origin [5,45] SMF repeatability (Noise)
X racks tray [1325,1360] Dev. Est. NC. F0 Covered ≤ 55
Y racks tray [320,350] Dev. Est. Abs. F1 340 nm ≤ 0.0004
X washing station [1325,1360] Dev. Est. Abs. F2 405 nm ≤ 0.0006
Y washing station [10,35] Dev. Est. Abs. F3 505 nm ≤ 0.0004
X rotor [2870,2910] Dev. Est. Abs. F8 670 nm ≤ 0.0007
Y rotor [660,700] Stability at 505 nm
Mazimum Z [170,200] Dev. Est. Abs. F3 505 nm ≤ 0.0008
Self-centering of needle Max. NC [840000,940000]
X Offset [-3,3] Min. NC [840000,940000]
Y Offset [-3,3] Max NC / Min NC ≤ 1.006
Level control scales Repeatability MF (Repeatability)
Water 0 L (0 D) [-3%,3%] Dev. Est. Abs. F1 340 nm ≤ 0.0008
Water 1.35 L (1 D) [35%,65%] Dev. Est. Abs. F2 405 nm ≤ 0.0006
Water 2.7 L (2 D) [95%,105%] Dev. Est. Abs. F3 505 nm ≤ 0.0005
Waste 0 L (0 D) [-3%,3%] Dev. Est. Abs. F4 535 nm ≤ 0.0005
Waste 1.35 L (1 D) [35%,65%] Dev. Est. Abs. F5 560 nm ≤ 0.0005
Waste 2.7 L (2 D) [95%,105%] Dev. Est. Abs. F6 600 nm ≤ 0.0005
Level detection sensitivity Dev. Est. Abs. F7 635 nm ≤ 0.0005
Paediatric rack [25,100] Dev. Est. Abs. F8 670 nm ≤ 0.0005
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Service manual
82
LA10418 CA10455 CA10456 FI10466 AC10770 AC11484
535
560
635 670
AC14556 AC14565
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Service manual
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Service manual
A V. SOFTWARE VERSIONS
Change in the versions of service program
Date version Change
3/06/03 1.3 First versions
14/04/03 1.3.2 Corrections
4/06/04 2.0.0 Improve the rotor verification test
Improve some display screen
Improve the test/change lamp
New screen: save and load the file adjustments
New initialitation screen. shows the state information of the analyser
09/07/04 2.5.0 Change the sensitivity adjust
20/03/06 3.0.0 New adjustment: adjust the maximum sweep of the z axis
New test of the maximum sweep of the z axis
Improve the level detection test
Improve the photometric test
Improve the stress test
New user’s menu
Improve the read/load adjustment and cycles
28/06/06 3.0.2 Correction of errors
Improve the checking before load a new firmware
30/11/06 3.1.0 Modify the level sensitivity adjustment screen to incorporate the in-
dependent adjustment by type of rack.
Added the help menu with the preventive maintenance guide.
14/12/07 3.2.0 Improve the fine zmaz adjustment. Include a new menu to change
the rotor type.
88
13/09/05 3.1.1 Calculated test
New programming test options:
Decreasing calibrations curve
Reactive blank with saline solution
New type of sample: Whole blood
20/03/06 3.2.0 Correction of Run Time errors
Improve the Base Line execution (repetion when it fails)
26/06/06 3.2.2 New button in the tools menu, reset the historical base line
Fix the options of the initial and final wash. Delete the menu to cho-
ose the parameter of initial and final wash.
14/11/06 3.3.0 Improve and optimitation of the execution times of the application.
Corrected the application errors that showed the message «not res-
pond»
1/03/07
2 3.3.2 Added the Korean language. Correction of Run Time errors.
14/06/07 4.0.0 Added the Romanian and indonesian languages
Added the substrate depletion control function in kinetic test
Added the prozone control function in turbidimetric test
Added the correlation of result per test with other analysers
Added graphical representation of results
Change the reagent Blank use in calibration factor calculation
Added a new patient results report
Added a new document explaining the Remarks messages
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Service manual
Compatibilities table
User A25 1.4.0 1.4.1 1.4.2 2.0.0 2.5.0 3.0.0 3.1.1 3.2.0 3.2.2 3.3.0 3.3.2 4.0.0
firmware
1.0 x x x x x
1.3 x x x x x
1.34 x x x x x
1.40 x x x
1.60 x
2.00 x
2.02 x
2.40 x x x x x x x
2.42 x x x x x x x
2.44 x x x x x x x
2.46 x x x x x x x
2.48 x x x x x x x
2.50 x x x x x x
2.54 x x x x x x
2.80 x x x x x x
2.94 x x x x x x
3.00 x x x x x x
3.15 x x x x x x
3.22 x x x x x x
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