mikrocount® dipslides

Hygiene testing made easy.

Trust is good, control is even better.

Systematic monitoring of the environmental and

operating hygiene status plays an important role
in quality assurance measures.

The mikrocount® product range provides every Step-by-step controls help to easily identify weak
operation with individual means of rapid and points in hygiene, so that necessary measures can
reliable hygiene control, e.g. in sensitive pro- quickly be initiated, thus ensuring that only goods
duction areas or medical areas. free of defects are produced at all times.
In addition to production and personnel hygiene
measures, applicable hygiene guidelines and The mikrocount® product series offer every op-
quality assurance concepts (e. g. GMP or HACCP) eration an uncomplicated means of rapid and
require routine hygiene controls at each step reliable hygiene control. The composition of the
of the production process and documen- agar surface was optimised for process moni-
tation of the results. toring, the control of raw and finished goods and
the control of disinfectant measures (surface
mikrocount® dipslides are the disinfection).
ideal solution for comprehen-
sive hygiene monitoring
of the entire production
process.

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All advantages at a glance.

Easy to use
No preparation time
Testing of surfaces, semi-solid materials
and liquids with just one product
Expensive laboratory equipment
is not necessary
Results within 24 – 72 hours

Fast and easy to use. Easy to evaluate.

Always at hand. Without any preparation time, A comparison with an evaluation chart allows
the slide which has an agar surface of 12.5 cm² the user to quickly and easily read off the results.
can be used to test surfaces, semisolid materials If necessary, the contaminated germ carrier can
and liquids. Special laboratory equipment and be used for specific follow-up tests.
personnel with microbiological training is not
necessary. In addition, the practical pivot joint Safety due to quality.
facilitates testing of surfaces. All mikrocount® dip- The strict standards to which mikrocount®
slides are equipped with a hexagonal hole in the dipslides are produced guarantee a product of
lid, which can be used for inserting an extension rod. consistent excellent quality. These standards
include clean-room filling and the ISO 9001 cer-
Economical.
tification. mikrocount® dipslides are supplied in
Sending away samples and time-con- shatter-free, environmentally friendly and sterile
suming laboratory work for preparing plastic tube made of polypropylene (without
nutrient media, production dilution PVC content).
series and counting colonies is
eliminated. Significant costs Easy disposal.
for an external laboratory
Once used, the dipslides can be destroyed by
are saved.
burning, autoclaving or immersion in disinfec-
tant solution.

mikrocount® product range:

mikrocount® dipslides are plastic nutrient mikrocount® dipslides are manufactured under
media slides coated on both sides with sterile conditions. The agar surfaces contain no
a special agar [1] on which the respec- toxic ingredients that could be transferred to the
[3] tive microorganisms grow. The high products being tested.
sensitivity of mikrocount® dipslides
[1] allows a hygiene control of products Furthermore, the dipslides are supplied with a
with high standards. transparent and shatter-proof protective tube [3].
The results are evaluated with the tube sealed,
The nutrient media slide is equip- thus avoiding the risk of contamination of em-
[2] ped with a pivot joint [2], making it ployees. Due to the high standards under which
considerably easier to draw samples mikrocount® dipslides are produced we guaran-
across the entire surface with surface tee a product of consistent excellent quality.
contact.

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 mikrocount® TPC
For the determination of the total plate count.
This nutrient media allows the growth of the most common bacteria,
yeasts and fungi. The agar contains lecithin and Tween for neutralising any
potential residues of disinfectants with cationic substances.
20 slides / box | Art. No. 182812

mikrocount® duo
For the combined determination of the total plate count as
well as yeasts and moulds.
mikrocount® duo is used for the combined determination of the total
plate count and for the detection of yeasts and moulds with inactivation of
disinfectants.
20 slides / box | Art. No. 70000724

mikrocount® TPC /E
For the combined determination of the total plate count and
for the detection of enterobacteria.
These nutrient media are used for the combined determination of the total
plate count and for the detection of enterobacteria within the scope of
microbiological monitoring of surfaces with inactivation of disinfectants.
20 slides / box | Art. No. 182811

Storage and
support
The mikrocount® dipslides should be
stored unopened at room temperature. They
should be protected from drying out, light and
frost. The dipslides have a shelf-life of 9 months.
Any unused slide exhibiting microbiological
growth should be discarded.
Temperature fluctuations may result in conden-
sate settling at the bottom of the slide tube.
This sterile liquid can simply be dis-
posed of down the sink, without
cultura® incubator the performance and results
of the dipslides.
For incubation of mikrocount® dipslides samples we
recommend the small cultura® incubator.
It is compact and adequate for nearly every laboratory or manufacturing site. It has room to
hold up to 18 mikrocount® dipslides. The transparent door allows to view the contents of
the incubator without having to move the samples. The temperature is adjustable and
preset by the manufacturer to maintain an optimal 30°C. Results for bacteria are available
after 24 to 48 hours. The detection of yeasts and moulds takes slightly longer (3 – 5 days).

Technical Data: cultura® incubator Art. No.

Capacity 18 mikrocount® dipslides 230 V 183301
Temperature range 25 – 45 °C (German plug)
External dimensions (w x d x h) 310 x 155 x 168 mm 230 V 183310
(English plug)
Internal dimensions (w x d x h) 220 x 120 x 150 mm
Weight 1.1 kg 110 V 183302
VDE/TÜV-proved

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Hygiene testing with a system.
Easy, safe and reliable – mikrocount® dipslides.

Application of mikrocount® dipslides

Contact inoculation: Streak test:

For surfaces and solid samples. Use a sterile swab to collect samples from points that are
Hold both sides of the nutrient media carrier onto the difficult to access or from highly viscous liquids.
surface for about 5 seconds without touching the agar The swab is then streaked out onto the agar surface
with your hands. of the slide in order to transfer the microorganisms to
The pivot joint of the agar slide offers additional be cultivated.
convenience.

Dip: Incubation:
The slide is dipped into the liquid which is to be tested Incubation in closed tubes
for a few seconds. mikrocount® TPC
Allow any excess liquid to run from the nutrient media 24 – 48 h at 25 °C – 30 °C
carrier to ensure safe transportation of the dipslides. mikrocount® TPC/E
24 – 48 h at 25 °C – 30 °C
mikrocount® duo
24 – 48 h at 25 °C – 30 °C
Detection of yeasts and moulds 3 – 5 days at 25 °C – 30 °C

Application areas:
hospital food processing
kitchens (e.g. hospitals)
cosmetic industry pharmaceutical industry
laboratories metalworking and related industry
paint industry paper and coatings industry
textile industry water treatment
household industry chemical / technical industry
applicable for tests according to DIN 55 997

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mikrocount® dipslides – Evaluation Tables

mikrocount® TPC Total plate count Bacteria

102 CFU/ml 103 CFU/ml 104 CFU/ml 105 CFU/ml 106 CFU/ml 107 CFU/ml

< 1 CFU/cm2 1 CFU/cm2 5 CFU/cm2 45 CFU/cm2 80 CFU/cm2 > 100 CFU/cm2

mikrocount® TPC/E Enterobacteria

103 CFU/ml 104 CFU/ml 105 CFU/ml 106 CFU/ml 107 CFU/ml

1 CFU/cm2 5 CFU/cm2 45 CFU/cm2 80 CFU/cm2 > 100 CFU/cm2

mikrocount® TPC/E Total plate count Bacteria

102 CFU/ml 103 CFU/ml 104 CFU/ml 105 CFU/ml 106 CFU/ml 107 CFU/ml

< 1 CFU/cm2 1 CFU/cm2 5 CFU/cm2 45 CFU/cm2 80 CFU/cm2 > 100 CFU/cm2

Please note that the colours shown may vary slightly from the colour change of the mikrocount® dipslides.

Evaluation of mikrocount® dipslides.

For the evaluation of the colony density on the carrier, compare the slides with another. However, different tests that use the same method for the same kind of
the pictures. Preferably, the evaluation should be done with a closed tube in order materials can be compared with one another and the results used for drawing
to prevent the contact with the microorganisms. Results that are obtained using quantitative conclusions. The nutrient media carrier for determining the total plate
varying inoculation methods of different materials cannot be compared with one count uses an agar that enables growth of the most common microorganisms.
*CFU = Colony Forming Unit
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mikrocount® dipslides – Evaluation Tables

mikrocount® duo Yeasts

102 CFU/ml 103 CFU/ml 104 CFU/ml 105 CFU/ml 106 CFU/ml

1 CFU/cm2 5 CFU/cm2 45 CFU/cm2 80 CFU/cm2 > 100 CFU/cm2

Destruction:
mikrocount® duo Fungi
Dipslides can be safely destroyed by
+ ++ +++ burning, autoclaving or by immer-
sion in a stock solution of a suitable
disinfectant
(10 % solution grotanol® 3025,
10 % solution perform® classic conc. GA,
4 % solution perform®.
Exposure time: overnight).

mikrocount® duo Total plate count Bacteria

102 CFU/ml 103 CFU/ml 104 CFU/ml 105 CFU/ml 106 CFU/ml 107 CFU/ml

< 1 CFU/cm2 1 CFU/cm2 5 CFU/cm2 45 CFU/cm2 80 CFU/cm2 > 100 CFU/cm2

Information:
For further information
please visit our product website
www.mikrocount.com

Most bacteria grow to form red colonies. Colourless colonies may also form, these Otherwise, bacteria exhibiting slow growth
must also be taken into consideration. It is advisable to read the results both after that did not exhibit visible growth on the first
one and also after two to three days. If the sample contains swarming Proteus day may sometimes occur. The detection limit
species or Bacillus species, it is best to read the results after the first day. of this agar is approx. >100 CFU*/ml.

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A company of the
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