Nihon Kohdem

MEK- 8222J SERVICE MANUAL
MEK- 8222K
AUTOMATED
HEMATOLOGY ANALYZER
MEK-8222
0634-001851B
Model: MEK-8222J/K
Manual code no.: 0634-001851B
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CONTENTS
Contents
GENERAL HANDLING PRECAUTIONS ................................................................................... i
WARRANTY POLICY .............................................................................................................. ii
RESPONSIBILITIES - PROFESSIONAL USERS ................................................................... ii
EMC RELATED CAUTION ...................................................................................................... iii
Conventions Used in this Manual and Instrument ................................................................... v
Warnings, Cautions and Notes ......................................................................................v
Explanations of the Symbols in this Manual and Instrument ........................................ vi
On panel ............................................................................................................. vi
On screen and recorded data ............................................................................ vii
Section 1 General .................................................................................. 1C.1

Introduction .......................................................................................................................... 1.1
Service Policy ...................................................................................................................... 1.2
Specifications ...................................................................................................................... 1.3
Measured Parameters, Ranges and Reproducibility to
Specimen from Venous Blood .......................................................................... 1.3
Detection Method ............................................................................................. 1.3
Standardization Analysis Method ..................................................................... 1.3
Interference Substances .................................................................................. 1.4
Dilution Ratio .................................................................................................... 1.6
Counting Time .................................................................................................. 1.6
Display ............................................................................................................. 1.6
Data Storage .................................................................................................... 1.6
Environmental Conditions ................................................................................. 1.6
Power Requirements ........................................................................................ 1.6
Dimensions and Weight .................................................................................... 1.7
Electromagnetic Compatibility .......................................................................... 1.7
Safety .............................................................................................................. 1.7
Panel Descriptions ................................................................................................................ 1.8
Front Panel ................................................................................................................. 1.8
Right Side Panel ......................................................................................................... 1.9
Rear Panel ............................................................................................................... 1.10
Inside Panels ........................................................................................................... 1.11
Composition ....................................................................................................................... 1.12
Demonstration Guide .......................................................................................................... 1.15
How to Upgrade the Software ............................................................................................. 1.16
Replacing the ROM ........................................................................................ 1.16
Upgrading the Software with the Memory Card .............................................. 1.19
Section 2 Troubleshooting ................................................................... 2C.1

Check Procedure Flowchart ................................................................................................. 2.1
Operating Condition and Blood Sample Checks ................................................................... 2.2
Service Manual MEK-8222 C.1

CONTENTS
Operating Condition Check ......................................................................................... 2.2

Blood Sample Handling Check ................................................................................... 2.2
Capillary Sample Preparation Check .......................................................................... 2.2
Atypical Blood Samples ............................................................................................. 2.3
Checking Particle CV ........................................................................................................... 2.8
Measuring Polymer Microsphere Suspensions for Checking CV ................................ 2.8
Poor Optical Reproducibility Problems ..................................................................... 2.10
No Scattergram and Histograms (TOC (Total Optical Count) is 0) .................. 2.10
Scattergrams and Histograms Appear But Low TOC (Below 2000) ................ 2.10
CV of FS or FL is Above 7% ......................................................................... 2.11
Adjusting the Flow Cell Unit Position ........................................................................ 2.11
Checking Background Noise .............................................................................................. 2.13
Measurement Procedure .......................................................................................... 2.13
Measurement ................................................................................................. 2.13
Results ........................................................................................................... 2.13
Background Noise Problems .................................................................................... 2.14
Checking the Reproducibility .............................................................................................. 2.15
About CV Values ...................................................................................................... 2.15
Checking Procedure ................................................................................................. 2.16
Poor Reproducibility Problems ................................................................................. 2.16
Poor WBC Reproducibility ............................................................................... 2.16
Poor WBC 5 Part Differential Reproducibility .................................................. 2.18
Poor HGB Reproducibility ............................................................................... 2.19
Poor RBC Reproducibility ............................................................................... 2.20
Poor PLT Reproducibility ................................................................................ 2.21
Poor HCT or MCV Reproducibility .................................................................. 2.23
Checking Accuracy ............................................................................................................ 2.24
Changing the Operator to “FACTORY” ...................................................................... 2.24
Calibration Procedure ............................................................................................... 2.24
Checking Scattergram ........................................................................................................ 2.26
Checking Scattergram Procedure ............................................................................. 2.26
Scattergram Problems ............................................................................................. 2.28
Differential Parameters Rough Gain Problems ............................................... 2.28
Differential Parameters Fine Gain Problems .................................................. 2.28
Solving Problems from Error Messages ............................................................................. 2.30
Error Messages ........................................................................................................ 2.30
A001: No diluent (type 1) ................................................................................ 2.30
A002: No diluent (type 2) ................................................................................ 2.30
A003: No diluent (flowcyte type 1) .................................................................. 2.31
A004: No diluent (flowcyte type 2) .................................................................. 2.31
A005: No detergent (CLEANAC) ..................................................................... 2.32
A006: No detergent (CLEANAC•3) ................................................................. 2.32
A007: No hemolysing reagent (HEMOLYNAC•3) ............................................ 2.32
A008: No hemolysing reagent (HEMOLYNAC•5) ............................................ 2.33
A009: WBC priming error ................................................................................ 2.33
A010: RBC priming error ................................................................................. 2.33
A021: WBC fluid level 1 .................................................................................. 2.34
A022: WBC fluid level 2 .................................................................................. 2.34
A023: WBC fluid level 3 .................................................................................. 2.34
A024: WBC bubble 1 ...................................................................................... 2.35
C.2 Service Manual MEK-8222

CONTENTS
A025: WBC bubble 2 ...................................................................................... 2.35

A026: WBC bubble 3 ...................................................................................... 2.35
A027: WBC bubble 4 ...................................................................................... 2.35
A028: WBC manomtr dirty .............................................................................. 2.35
A029: WBC clogged ....................................................................................... 2.36
A030: WBC sample error ................................................................................ 2.36
A031: WBC hardware noise ............................................................................ 2.36
A032: WBC software noise ............................................................................. 2.36
A041: RBC fluid level 1 .................................................................................. 2.37
A042: RBC fluid level 2 .................................................................................. 2.37
A043: RBC fluid level 3 .................................................................................. 2.37
A044: RBC bubble 1 ....................................................................................... 2.38
A045: RBC bubble 2 ....................................................................................... 2.38
A046: RBC bubble 3 ....................................................................................... 2.38
A047: RBC bubble 4 ....................................................................................... 2.38
A048: RBC manomtr dirty .............................................................................. 2.38
A049: RBC clogged ........................................................................................ 2.39
A050: RBC sample error ................................................................................. 2.39
A051: RBC hardware noise ............................................................................. 2.39
A052: RBC software noise ............................................................................. 2.39
A061: HGB voltage low .................................................................................. 2.40
A062: HGB voltage high ................................................................................. 2.40
A063: HGB circuit error .................................................................................. 2.40
A071: Mixing error .......................................................................................... 2.40
A081: Laser switch off .................................................................................... 2.41
A082: Optical count error ............................................................................... 2.41
System Error Messages ........................................................................................... 2.42
E032: Circuit error .......................................................................................... 2.42
E033: Check settings ..................................................................................... 2.42
E101: CLOSED SAMPLER ERROR .............................................................. 2.42
E102: OPEN SAMPLER ERROR ................................................................... 2.42
E103: TABLE ERROR .................................................................................... 2.42
E104: MIXING ERROR .................................................................................. 2.42
E105: BARCODE ERROR .............................................................................. 2.43
E106: DILUTER ERROR ................................................................................ 2.43
E107: LYSE PUMP ERROR ........................................................................... 2.43
E108: SAMPLE PUMP ERROR ..................................................................... 2.43
E109: SHEATH PUMP ERROR ...................................................................... 2.43
E110: 5DIFF ROTARY PUMP ERROR ........................................................... 2.43
E111: WBC ROTARY PUMP ERROR ............................................................. 2.44
E112: RBC ROTARY PUMP ERROR .............................................................. 2.44
E113: WBC SUB BATH ERROR ..................................................................... 2.44
E114: RBC SUB BATH ERROR ..................................................................... 2.44
E115: WARMER ERROR ................................................................................ 2.44
E201: MASTER BD ERROR .......................................................................... 2.44
E202: SLAVE MS BD ERROR ....................................................................... 2.44
E203: SLAVE CBC BD ERROR ..................................................................... 2.45
E301: MASTER-MS COMM ERROR ............................................................. 2.45

CONTENTS
E302: MASTER-CBC1 COMM ERROR .......................................................... 2.45

E303: MASTER-CBC2 COMM ERROR .......................................................... 2.45
E304: MS-CBC COMM ERROR ..................................................................... 2.45
E305: MS-BARCODE COMM ERROR ........................................................... 2.45
Maintenance Information .......................................................................................... 2.46
Solving Problems from Flags ............................................................................................. 2.47
Blasts and Immature Granulocyte .................................................................. 2.47
Left Shift ........................................................................................................ 2.47
Atypical Lymphocytes .................................................................................... 2.48
! appears on the right of WBC measured value .............................................. 2.48
! appears on the right of MCHC measured value ............................................ 2.49
C appears on the right of WBC or PLT measured value .................................. 2.49
Service Maintenance Screens ............................................................................................ 2.51
Displaying the SERVICE MAINTENANCE Screen ................................................... 2.51
CHECK & MAINTENANCE Screen .......................................................................... 2.52
MEASURE WBC & RBC ................................................................................ 2.52
MEASURE WBC ............................................................................................ 2.52
MEASURE RBC ............................................................................................ 2.52
CIRCUIT CHECK ........................................................................................... 2.53
PRIME BATHS ............................................................................................... 2.53
UNIT CLEAN .................................................................................................. 2.53
DRAIN BATHS ............................................................................................... 2.53
REMOVE FLOWCELLS ................................................................................. 2.53
LIQUID TANK PRIME ..................................................................................... 2.53
BACKUP RAM CHECK .................................................................................. 2.53
SAMPLE UNIT MAINTENANCE Screen .................................................................. 2.54
MS-820V ........................................................................................................ 2.54
MS-821V ........................................................................................................ 2.55
MS-822V ........................................................................................................ 2.55
CLEANING NEEDLE...................................................................................... 2.56
PUMP UNIT MAINTENANCE Screen ...................................................................... 2.56
MP-520V 5DIFF, MP-520V WBC, MP-520V RBC ........................................... 2.56
MP-820V ........................................................................................................ 2.57
MP-821V ........................................................................................................ 2.58
VALVE & PUMP CHECK ............................................................................... 2.59
CONTINUOUS MEASUREMENT Screen ................................................................. 2.60
CLOSED MODE 10 SAMPLES ...................................................................... 2.60
CLOSED MODE 30 SAMPLES ...................................................................... 2.60
RACK 1, 2 40 SAMPLES ............................................................................... 2.60
MANUAL MODE CONT MEAS. ..................................................................... 2.60
CLOSED MODE CONT MEAS. ..................................................................... 2.60
MONITOR Screen .................................................................................................... 2.61
SENSOR MONITOR ...................................................................................... 2.61
SCI MONITOR ............................................................................................... 2.62
POS SENSOR MONITOR .............................................................................. 2.62
10 DATA X-CV Screen .............................................................................................. 2.63
INITIALIZATION MENU Screen ............................................................................... 2.63
ADVANCED SETTINGS Screen ............................................................................... 2.64
MEASUREMENT MODE ............................................................................... 2.65

CONTENTS
UNIT SETTINGS ........................................................................................... 2.66

SCREEN SETTINGS ..................................................................................... 2.67
FLAGS SETTINGS ........................................................................................ 2.67
MAINTENANCE ALARM ................................................................................ 2.69
Modification Procedure for Other Sample Tubes ................................................................. 2.70
Optional Racks for S-Monovette and KABE sample tubes ....................................... 2.73
Section 3 Board/Unit Description ........................................................ 3C.1

Sub Bath Unit ....................................................................................................................... 3.1
CBC Measuring Unit ............................................................................................................. 3.1
Laser Optical Unit ................................................................................................................. 3.1
Complex Pump Unit ............................................................................................................. 3.1
Sample Pump Unit ............................................................................................................... 3.2
Sheath Pump Unit ................................................................................................................ 3.2
Closed Sampler Unit ............................................................................................................. 3.2
Open Sampler Unit ............................................................................................................... 3.2
Table Unit ............................................................................................................................. 3.2
Display Unit .......................................................................................................................... 3.2
MASTER Board .................................................................................................................... 3.3
SLAVE CBC Board ............................................................................................................... 3.3
SLAVE MS Board ................................................................................................................. 3.3
Section 4 Disassembly and Assembly ............................................... 4C.1

Before You Begin .................................................................................................................. 4.1
Warnings and Cautions ............................................................................................... 4.1
Required Tools ............................................................................................................ 4.1
Caution and Notes Related to Valve Joint,
Black Screw and Tube Joint in the Instrument ............................................................ 4.2
Board and Unit Location ............................................................................................. 4.4
Removing the Front, Right Side, Left Side, Top and Rear Covers ......................................... 4.5
Removing the Display Unit ................................................................................................... 4.7
Removing the Laser Optical Unit .......................................................................................... 4.8
Removing the Flow Cell Unit ................................................................................................ 4.9
Removing the Closed Sample Unit ..................................................................................... 4.10
Removing the Open Sampler Unit ...................................................................................... 4.11
Removing the Table Unit ..................................................................................................... 4.12
Removing the CBC Measuring Unit .................................................................................... 4.13
Removing the Sub Bath Unit .............................................................................................. 4.14
Removing the Pump Units under the CBC Measuring Unit ................................................. 4.15
Removing the Pump Unit from the Right Side of the Closed Sampler Unit ......................... 4.16
Removing the Complex Pump Unit ..................................................................................... 4.17
Removing the Sample Pump Unit ...................................................................................... 4.18
Removing the Sheath Pump Unit ....................................................................................... 4.19
Removing the 4-channel Liquid Sensor Unit ....................................................................... 4.20
Removing the Power Supply Unit ....................................................................................... 4.21
Removing the SLAVE CBC Board ...................................................................................... 4.21
Removing the SLAVE MS Board ........................................................................................ 4.22

CONTENTS
Removing the MASTER Board ........................................................................................... 4.22

Removing the POWER Board ............................................................................................. 4.23
Removing the DRIVER1 Board .......................................................................................... 4.24
Removing the INTERFACE Board ...................................................................................... 4.28
Section 5 Socket Pin Assignment ....................................................... 5C.1

Socket Pin Assignment ........................................................................................................ 5.1
RS-232C Socket .............................................................................................. 5.1
ZK-820V Socket .............................................................................................. 5.1
USB Socket ..................................................................................................... 5.1
Section 6 Adjustment............................................................................ 6C.1

Adjusting the Flow Cell Position ........................................................................................... 6.1
Adjusting the FS/FL/SD Gain and FS Threshold .................................................................. 6.4
Adjusting Sensors ................................................................................................................ 6.7
SENSOR MONITOR screen ....................................................................................... 6.7
How to call up the SENSOR MONITOR screen ............................................... 6.7
CBC Manometer ......................................................................................................... 6.8
WBC manometer upper sensor adjustment ...................................................... 6.9
WBC manometer lower sensor adjustment ....................................................... 6.9
RBC manometer upper sensor adjustment ....................................................... 6.9
RBC manometer lower sensor adjustment ....................................................... 6.9
CBC Electrode ......................................................................................................... 6.10
HGB Sensor ............................................................................................................. 6.11
HGB sensor adjustment ................................................................................. 6.11
Calibration Switch .................................................................................................... 6.12
Liquid Sensor ........................................................................................................... 6.13
4-channel liquid sensor unit adjustment ......................................................... 6.15
Pressure Sensor ...................................................................................................... 6.19
Temperature Sensor ................................................................................................. 6.20
Setting on the UT-7159 WARMER CONTROL board ....................................... 6.21
Calibrating the Touch Screen .............................................................................................. 6.22
Section 7 Maintenance ......................................................................... 7C.1

YZ-0296 Consumable Replacement Parts List in Periodic Maintenance Check ................... 7.1
Procedure before Checking, Cleaning or Replacing a Periodic Replacement Part in the
Hematology Analyzer ........................................................................................................... 7.2
Checking, Cleaning or Replacing the Rinse Unit, Sampling Nozzle and Cap Pierce Nozzle .....
for Closed Mode ................................................................................................................... 7.4
Checking or Replacing the Manual Sampling Nozzle ............................................................ 7.6
Checking and Cleaning the Manual Rinse Unit ..................................................................... 7.7
Checking and Cleaning or Replacing Filters ......................................................................... 7.9

CONTENTS
Checking and Cleaning the Sub Baths, Measurement Baths and Sample Cup .................. 7.10
Cleaning the Aperture ......................................................................................................... 7.11
Checking and Cleaning the Waste Cup ............................................................................... 7.13
Checking and Replacing the Pump Tubes ........................................................................... 7.14
Checking and Cleaning the Mixing Unit .............................................................................. 7.16
Checking or Replacing the Pinch Valve Tube ...................................................................... 7.17
Priming after Periodic Maintenance Check, Storage or Transport ....................................... 7.18
Section 8 Replaceable Parts List......................................................... 8C.1

General ................................................................................................................................. 8.1
Unit Location ........................................................................................................................ 8.2
Board Location ..................................................................................................................... 8.4
Exploded View-1 ................................................................................................................... 8.6
Exploded View-2 ................................................................................................................... 8.8
Exploded View-3 ................................................................................................................. 8.10
Section 9 Demonstration Guide .......................................................... 8C.1

Introduction .......................................................................................................................... 9.1
Demonstration Outline ................................................................................................ 9.1
Required Items for Demonstration .............................................................................. 9.2
Preparing the Hematology Analyzer ..................................................................................... 9.4
Installation Flowchart ................................................................................................. 9.4
Connecting Tubes ....................................................................................................... 9.4
Connecting the Power Cord and Grounding the Hematology Analyzer ........................ 9.6
Connecting the Power Cord .............................................................................. 9.6
Equipotential Grounding ................................................................................... 9.6
Turning the Laser Switch On ...................................................................................... 9.6
Turning On the Power ................................................................................................. 9.7
Setting Date & Time and Priming the Hematology Analyzer ................................................. 9.8
Setting the Date and Time .......................................................................................... 9.8
Priming the Fluid Path ................................................................................................ 9.9
Adjusting Gain and Measuring Background Noise .............................................................. 9.10
Flowchart ................................................................................................................. 9.10
Reference: Principle of Differentiating WBC ............................................................. 9.10
Adjusting Gain in Rough Mode ................................................................................. 9.11
Adjusting Gain in Rough Mode and Measuring Polymer
Microsphere Suspensions .................................................................... 9.11
Adjusting the Flow Cell Unit Position .............................................................. 9.12
Measuring Background Noise ................................................................................... 9.14
Adjusting Gain in Fine Mode .................................................................................... 9.15
Checking the Gain Adjustment ................................................................................. 9.20
Calibrating the Hematology Analyzer .................................................................................. 9.22
Procedure Flowchart ................................................................................................ 9.22
Changing the Operator ............................................................................................. 9.22
Calibrating CBC Parameters ..................................................................................... 9.23
Checking the Calibration Coefficients for WBC 5 Part Differential Parameters ......... 9.26
Changing the Operator Back to a LAB TECHNICIAN ............................................... 9.26
Checking the Data .............................................................................................................. 9.27

CONTENTS
Check with MEK-5D Hematology Control ................................................................. 9.27

Check with Venous Blood Samples of a Healthy Person .......................................... 9.28
Interference Substances .................................................................................................... 9.30
Storing and Transporting the Hematology Analyzer ............................................................ 9.33
About the Optional ZK-821V/VG Bar Code Reader ............................................................. 9.35
Checking the Bar Code Reader Function .................................................................. 9.35
Using Bar Codes ...................................................................................................... 9.36

GENERAL HANDLING PRECAUTIONS
This device is intended for use only by qualified medical personnel.

Use only Nihon Kohden approved products with this device. Use of non-approved products or in
a non-approved manner may affect the performance specifications of the device. This includes,
but is not limited to, batteries, recording paper, pens, extension cables, electrode leads, input
boxes and AC power.
Please read these precautions thoroughly before attempting to operate the instrument.
1. To safely and effectively use the instrument, its operation must be fully understood.
2. When installing or storing the instrument, take the following precautions:

(1) Avoid moisture or contact with water, extreme atmospheric pressure, excessive humidity and temperatures, poorly
ventilated areas, and dust, saline or sulphuric air.
(2) Place the instrument on an even, level floor. Avoid vibration and mechanical shock, even during transport.
(3) Avoid placing in an area where chemicals are stored or where there is danger of gas leakage.
(4) The power line source to be applied to the instrument must correspond in frequency and voltage to product
specifications, and have sufficient current capacity.
(5) Choose a room where a proper grounding facility is available.
3. Before Operation
(1) Check that the instrument is in perfect operating order.
(2) Check that the instrument is grounded properly.
(3) Check that all cords are connected properly.
(4) Pay extra attention when the instrument is in combination with other instruments to avoid misdiagnosis or other
problems.
(5) All circuitry used for direct patient connection must be doubly checked.
(6) Check that battery level is acceptable and battery condition is good when using battery-operated models.
4. During Operation
(1) Both the instrument and the patient must receive continual, careful attention.
(2) Turn power off or remove electrodes and/or transducers when necessary to assure the patient’s safety.
(3) Avoid direct contact between the instrument housing and the patient.
5. To Shutdown After Use

(1) Turn power off with all controls returned to their original positions.
(2) Remove the cords gently; do not use force to remove them.
(3) Clean the instrument together with all accessories for their next use.
6. The instrument must receive expert, professional attention for maintenance and repairs. When the instrument is not
functioning properly, it should be clearly marked to avoid operation while it is out of order.
7. The instrument must not be altered or modified in any way.
8. Maintenance and Inspection:

(1) The instrument and parts must undergo regular maintenance inspection at least every 6 months.
(2) If stored for extended periods without being used, make sure prior to operation that the instrument is in perfect
operating condition.
Service Manual MEK-8222 i

9. When the instrument is used with an electrosurgical instrument, pay careful attention to the application and/or
location of electrodes and/or transducers to avoid possible burn to the patient.
10. When the instrument is used with a defibrillator, make sure that the instrument is protected against defibrillator
discharge. If not, remove patient cables and/or transducers from the instrument to avoid possible damage.
WARRANTY POLICY
Nihon Kohden Corporation (NKC) shall warrant its products against all defects in materials and workmanship for one year
from the date of delivery. However, consumable materials such as recording paper, ink, stylus and battery are excluded from
the warranty.
NKC or its authorized agents will repair or replace any products which prove to be defective during the warranty period,
provided these products are used as prescribed by the operating instructions given in the operator’s and service manuals.
No other party is authorized to make any warranty or assume liability for NKC’s products. NKC will not recognize any other
warranty, either implied or in writing. In addition, service, technical modification or any other product change performed by
someone other than NKC or its authorized agents without prior consent of NKC may be cause for voiding this warranty.
Defective products or parts must be returned to NKC or its authorized agents, along with an explanation of the failure.
Shipping costs must be pre-paid.
This warranty does not apply to products that have been modified, disassembled, reinstalled or repaired without Nihon
Kohden approval or which have been subjected to neglect or accident, damage due to accident, fire, lightning, vandalism,
water or other casualty, improper installation or application, or on which the original identification marks have been
removed.
In the USA and Canada other warranty policies may apply.
RESPONSIBILITIES - PROFESSIONAL USERS

This instrument must be used by a professional user with a full knowledge of operating this instrument, only for his/her
intended use and according to the instructions for use. Instructions in the operator’s manual must be followed, especially the
following points.
• Storage and stability of reagents
• Handling of reagents
• Instrument installation
• Connection of all tubes to inlets and outlets
• Connection of all tubes to reagents and waste container
• Checking the amount of reagents and waste fluid
• Calibration
• Quality control
• Maintaining and servicing
If deviating from the instructions, the professional user does it at the risk and liability of the laboratory and only after
validation by the laboratory. Nihon Kohden has no responsibility over such deviations.
ii Service Manual MEK-8222

EMC RELATED CAUTION
This equipment and/or system complies with the International Standard EN 61326-1 for electromagnetic
compatibility for electrical equipment and/or system for measurement, control and laboratory use. However,
an electromagnetic environment that exceeds the limits or levels stipulated in the EN 61326-1, can cause
harmful interference to the equipment and/or system or cause the equipment and/or system to fail to
perform its intended function or degrade its intended performance. Therefore, during the operation of the
equipment and/or system, if there is any undesired deviation from its intended operational performance, you
must avoid, identify and resolve the adverse electromagnetic effect before continuing to use the equipment
and/or system.
The following describes some common interference sources and remedial actions:
1. Strong electromagnetic interference from a nearby emitter source such as an authorized radio station or
cellular phone:
Install the equipment and/or system at another location if it is interfered with by an emitter source such
as an authorized radio station. Keep the emitter source such as cellular phone away from the equipment
and/or system.
2. Radio-frequency interference from other equipment through the AC power supply of the equipment and/
or system:
Identify the cause of this interference and if possible remove this interference source. If this is not
possible, use a different power supply.
3. Effect of direct or indirect electrostatic discharge:

Make sure all users and patients in contact with the equipment and/or system are free from direct or
indirect electrostatic energy before using it.
4. Electromagnetic interference with any radio wave receiver such as radio or television:
If the equipment and/or system interferes with any radio wave receiver, locate the equipment and/or
system as far as possible from the radio wave receiver.
If the above suggested remedial actions do not solve the problem, consult your Nihon Kohden Corporation
subsidiary or distributor for additional suggestions.
This equipment complies with International Standard EN55011 (1999) Group 1, Class B. Class B
EQUIPMENT is equipment suitable for use in domestic establishments and in establishments directly
connected to a low voltage power supply network which supplies buildings used for domestic purposes.
The CE mark is a protected conformity mark of the European Community. The products herewith comply
with the requirements of the IVD Directive 98/79/EEC.
Service Manual MEK-8222 iii

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iv Service Manual MEK-8222

Conventions Used in this Manual and Instrument
Warnings, Cautions and Notes
Warnings, cautions and notes are used in this manual to alert or signal the reader to specific information.
WARNING
A warning alerts the user to the possible injury or death associated with the use or misuse of the
instrument.
CAUTION
A caution alerts the user to possible injury or problems with the instrument associated with its use or
misuse such as instrument malfunction, instrument failure, damage to the instrument, or damage to other
property.
NOTE
A note provides specific information, in the form of recommendations, prerequirements, alternative methods
or supplemental information.
Service Manual MEK-8222 v

Explanations of the Symbols in this Manual and Instrument
The following symbols found in this manual/instrument bear the respective descriptions as given.
On panel
Symbol Description Symbol Description
HEMOLYNAC•3
Main power lamp
(hemolysing reagent)
HEMOLYNAC•3N*
“On” only for part of the equipment
HEMOLYNAC•5
“Off” only for part of the equipment
Laser ON WASTE
Hand positions for carrying the

Reset
instrument
Manual RS-232C socket
Clean 2 ZK-820V bar code reader socket
Eject USB socket
Start IVD IVD conformity
Emergency Fuse
Do not touch the sample rack AC power off

during measurement (Disconnection from the mains)
AC power on
Inlet
(Connection to the mains)
Outlet Alternating current
Attention, consult operator's manual Equipotential terminal
Biohazard Year of manufacture
ISOTONAC•3
Serial number
(diluent)
The CE mark is a protected
CLEANAC
conformity mark of the European
(detergent)
Community. The products
herewith comply with the
CLEANAC•3
requirements of the IVD Directive
(detergent)
98/79/EEC.
* When using the HEMOLYNAC•3N hemolysing reagent, the HEMO3N label is to be attached over the HEMO3 label.
vi Service Manual MEK-8222
On screen and recorded data
Symbol Description Symbol Description
Beside WBC or RBC measured
value: Sample error Beside HGB measured value: HGB
Beside HGB measured value: Dirty circuit error
measurement baths
Beside WBC measured value: Poor
hemolyzation
Beside HGB measured value: HGB C Beside WBC or PLT measured
value: Platelet coagulation
voltage adjustment error
Service Manual MEK-8222 vii

Section 1 General
Introduction ........................................................................................................................ 1.1

Service Policy ................................................................................................................... 1.2
Specifications .................................................................................................................... 1.3
Measured Parameters, Ranges and Reproducibility to
Specimen from Venous Blood ....................................................................... 1.3
Detection Method .......................................................................................... 1.3
Standardization Analysis Method ................................................................. 1.3
Interference Substances ............................................................................... 1.4
Dilution Ratio ................................................................................................. 1.6
Counting Time ............................................................................................... 1.6
Display .......................................................................................................... 1.6
Data Storage ................................................................................................. 1.6
Environmental Conditions ............................................................................. 1.6
Power Requirements ..................................................................................... 1.6
Dimensions and Weight ................................................................................. 1.7
Electromagnetic Compatibility ...................................................................... 1.7
Safety ............................................................................................................ 1.7
Panel Descriptions ............................................................................................................ 1.8
Front Panel .............................................................................................................. 1.8
Right Side Panel ...................................................................................................... 1.9
Rear Panel ..............................................................................................................1.10
Inside Panels .......................................................................................................... 1.11
Composition ...................................................................................................................... 1.12
Demonstration Guide ....................................................................................................... 1.15
How to Upgrade the Software ............................................................................................ 1.16
Replacing the ROM ....................................................................................... 1.16
Upgrading the Software with the Memory Card ............................................. 1.19
Service Manual MEK-8222 1C.1

1. GENERAL
Introduction
CAUTION
To maintain the instrument in normal condition, the user must perform
the periodic maintenance. Refer to “Maintenance” of the operator’s
manual.
This service manual provides useful information to qualified service personnel to

understand, troubleshoot, service, maintain and repair the MEK-8222 Hematology
Analyzer (referred to as “the instrument” in this service manual).
All replaceable parts or units of this instrument and its optional units are clearly
listed with exploded illustration to help you locate the parts quickly.
The maintenance must be periodically performed because the instrument has fluid
paths and precision parts. Accordingly, the user is responsible for performing the
periodic maintenance. The “Maintenance” section in this service manual
describes the maintenance that should be performed by qualified service
personnel. The “Maintenance” section in the operator’s manual describes the
maintenance that can be performed by the user.
NOTE
If the instrument has a problem and there has been no periodic
maintenance, the instrument will usually be normal again by cleaning
the fluid paths or replacing a consumable with a new one.
The information in the operator’s manual is primarily for the user. However, it is
important for service personnel to thoroughly read the operator’s manual and
service manual before starting to troubleshoot, service, maintain or repair this
instrument. This is because service personnel needs to understand the operation
of the instrument in order to effectively use the information in the service manual.
Service Manual MEK-8222 1.1

1. GENERAL
Service Policy
CAUTION
• Be careful not to directly touch any place where blood is or may
spread to.
• Wear rubber gloves to protect yourself from infection before doing
maintenance.
Nihon Kohden Corporation’s basic policy for technical service is to replace faulty
units, printed circuit boards or parts. We do not support component-level repair of
boards and units outside the factory.
NOTE
• When ordering parts or accessories from your nearest Nihon Kohden
Corporation’s distributor, please quote the NK code number and part
name which is listed in this service manual, and the name or model
of the unit in which the required part is located. This will help us to
promptly attend to your needs.
• Always use parts and accessories recommended or supplied by
Nihon Kohden Corporation to assure maximum performance from
your instrument.
1.2 Service Manual MEK-8222

1. GENERAL
Specifications
Measured Parameters, Ranges and Reproducibility to Specimen from Venous Blood

Specifications were determined using hematology control blood (MEK-3DN), counted 10 times consecutively.
Reproducibility to Specimen from Venous
Measured Parameters Measuring Range Blood
(CV: Coefficient of Variation)
0 to 299 × 10 /µL within 2.0%CV (4.0 to 9.0 × 10 /µL)
3 3
WBC: White blood cell count
within 5.0%CV (WBC: 4.0 to 9.0 × 10 /µL,
3
NE%: Neutrophil percent 0 to 99.9%

NE%: 40 to 70%)
3
LY%: Lymphocyte percent 0 to 99.9%

LY%: 20 to 45%)
3
MO%: Monocyte percent 0 to 99.9%

MO%: 2 to 10%)
3
EO%: Eosinophil percent 0 to 99.9%

EO%: 2 to 10%)
within CV30.0% (>2%) or average value ±1% (0 to
BA%: Basophil percent 0 to 99.9%
2%) (WBC: 4.0 to 9.0 × 10 /µL, BA%: 0 to 3%)
3
0 to 299 × 10 /µL
3
NE: Neutrophil count
0 to 299 × 10 /µL
3
LY: Lymphocyte count
0 to 299 × 10 /µL
3
MO: Monocyte count
0 to 299 × 10 /µL
3
EO: Eosinophil count
0 to 299 × 10 /µL
3
BA: Basophil count
0 to 14.9 × 10 /µL within 1.5%CV (5.0 × 10 /µL)
6 6
RBC: Red blood cell count
HGB: Hemoglobin concentration 0 to 29.9 g/dL within 1.5%CV (16 g/dL)
HCT: Hematocrit 0 to 99.9%
MCV: Mean cell volume 20 to 199 fL within 1.0%CV (70 to 120 fL)
MCH: Mean cell hemoglobin 10 to 50 pg
MCHC: Mean cell hemoglobin concentration 10 to 50 g/dL
RDW: Red blood cell distribution width 0 to 50%
0 to 1490 × 10 /µL within 4.0%CV (3.0 × 10 /µL)
3 3
PLT: Platelet count
PCT: Platelet crit 0 to 2.9%
MPV: Mean platelet volume 0 to 20.0 fL
PDW: Platelet distribution width 0 to 50%
Detection Method
Blood cell count: Electrical resistance detection
Hemoglobin: Cyanmethemoglobin optical detection
Hematocrit: Histogram calculation
WBC population: Light scatter by laser
Platelet crit: Histogram calculation
RBC distribution width: Histogram calculation
Platelet distribution width: Histogram calculation
Standardization Analysis Method

WBC: ICSH1988 ICSH: The assignment of values to fresh blood used for calibrating automated blood cell counters. Clin
Lab Haematol, 10:203-212, 1988
RBC: ICSH1988 ICSH: The assignment of values to fresh blood used for calibrating automated blood cell counters. Clin
Lab Haematol, 10:203-212, 1988

1. GENERAL
HGB: NCCLS H15-A2 H15-A2: Reference and Selected Procedures for the Quantitative Determination of Hemoglobin in
Blood Second Edition; Approved Standard (1994)
HCT: NCCLS H7-A2 H7-A2: Procedure for Determining Packed Cell Volume by the Microhematocrit Method Second
Edition; Approved Standard (1993)
PLT: Brecher & Cronkite
Interference Substances
WBC: Unlysed red cells
In some rare occasions, the RBC in the blood sample may not completely lyse and these non-lysed RBC
may be detected as WBC and cause increase in WBC count.
Multiple myeloma
The precipitation of proteins in multiple myeloma patients may increase the WBC count.
Leukemia
WBC is fragile in leukemia patient and WBC may be destroyed during measurement. These WBC
fragments may also interfere with WBC differential measurement.
Chemotherapy
Cytotoxic and immunosuppressive drugs cause low WBC count.
Cryoglobulins
Cryoglobulin may be increased in patients who are or have myeloma, cancer, leukemia, macroglobulinemia,
lymphoproliferative disorders, metastatic tumors, autoimmune disorders, infections, anerurism, pregnant,
thromboembolic phenomena, diabetes, etc, which cause increase in WBC, RBC or PLT counts and HGB
concentration. In such cases, warm the blood sample to 37°C in a water bath for 30 minutes and measure
the sample immediately.
RBC: Leukemia
An increase in WBC in leukemia patient causes increase in RBC.
Agglutinated RBC
Agglutinated RBC may decrease RBC count. This can be checked by abnormal MCH and MCHC values
and examination of the stained blood film.
Cold agglutinins
IgM immunoglobulins which are elevated in clod agglutinin disease may decrease RBC and PLT counts and
increase MCV.
HGB: Turbidity of the blood sample
Any physiologic and/or therapeutic factors may increase HGB. In such a case, determine the cause of
turbidity and follow the appropriate method below.
1. Increased WBC
An extreme increase in WBC will cause excessive light scatter. In these cases, measure manually.
Centrifuge the diluted sample and measure the supernatant fluid with a spectrophotometer.
2. Increased lipids
The blood sample may be milky when there is excessive lipid. This may occur with hyperlipidemia,
hyperproteinemia and hyperbilirubinemia. Accurate HGB measurement can be achieved by manual
methods and a plasma blank.
3. Increased turbidity
When RBC are resistant to lysing, turbidity may increase causing increase in HGB. Observe if MCH
and MCHC values are abnormal. HGB result affects MCH and MCHC result.
4. Fetal bloods
The mixing of fetal and maternal blood may increase HGB value.
HCT: Agglutinated RBC
RBC agglutination may cause erroneous HCT and MCV values. Observe if MCH and MCHC values are
abnormal. In such a case, measure manually.

1. GENERAL
MCV: Agglutinated RBC

Excessive number of large PLT
Excessive number of large PLT and/or excessively high WBC may affect the MCV value. Check by careful
examination of the stained blood film.
MCH: MCH is determined from HGB and RBC values. Therefore, the limitations for HGB and RBC also affect
MCH value.
MCHC: MCHC is determined from HGB and HCT values. Therefore, the limitations for HGB and HCT also affect
MCHC value.
RDW: RDW is determined from RBC value. Therefore, the limitations for RBC also affect RDW value.
Agglutinated RBC
Agglutinated RBC may decrease RBC count and erroneous RDW. This can be checked by abnormal MCH
and MCHC values and examination of the stained blood film.
Nutritional deficiency or blood transfusion
Iron and/or cobalamin and/or folate deficiency may increase RDW.
PLT: Very small fragments
Very small RBC, RBC fragments and WBC fragments may be the cause in increased PLT count.
Agglutinated RBC
PLT may be trapped in the agglutinated RBC resulting in decrease in PLT. This can be checked by abnormal
MCH and MCHC values and examination of the stained blood film.
Very large PLT
Large PLT may exceed the PLT threshold and may not be counted which results in low PLT count.
Chemotherapy
Cytotoxic and immunosuppressive drugs may increase the fragility of cells which may cause low PLT count.
In such a case, measure manually.
Hemolysis
Hemolyzed specimens contain red cell stroma which may increase PLT count.
Anticoagulated blood
Blood anticoagulated with acid-citrate-dextrose may have clumped PLT which may cause decrease in PLT
count.
Agglutinated PLT
Clumped PLT may decrease PLT count and/or increase WBC count. For such sample, collect the sample in
sodium citrate anticoagulant and measure only PLT. The PLT result must be corrected for the sodium citrate
dilution effect.
MPV: Very large PLT
Large PLT may exceed the PLT threshold and not be counted which results in low MPV.
Very small fragments
Very small RBC, RBC fragments and WBC fragments may interfere with MPV measurement.
Agglutinated RBC
PLT may be trapped in the agglutinated RBC resulting erroneous MPV. This can be checked by abnormal
Chemotherapy
Cytotoxic and immunosuppressive drugs may affect MPV. In such a case, measure manually.
NOTE
Blood samples collected in EDTA does not maintain stable MPV because platelets swell
depending on the interval after collection and storage temperature.

1. GENERAL
WBC 5 part differential parameters are derived from the WBC count, therefore, the limitations for WBC also affect these
parameters.
LY and LY%: Erythroblasts, certain parasites and RBC that are resistant to lysis may interfere with an accurate LY count.
MO and MO%: Large lymphocytes, atypical lymphocytes, blasts and excessive number of basophils may interfere with
an accurate MO count.
NE and NE%: Excessive eosinophils, metamyelocytes, myelocytes, promyelocytes, blasts and plasma cells may interfere
with an accurate NE count and NE%.
EO and EO%: Abnormal granules may interfere with an accurate EO count.
BA and BA%: Immature cell, metamyelocytes, myelocytes, promyelocytes, blasts and plasma cells may interfere with an
accurate BA count and BA%.
Dilution Ratio
• Venous blood
Sample volume: 55 µL
WBC/HGB: 200:1
RBC/PLT: 40,000:1
• Capillary blood
Sample volume: 10 µL 20 µL
WBC/HGB: 1200:1 600:1
RBC/PLT: 240,000:1 120,000:1
Counting Time
Approximately 45 s/sample, 80 samples/hr
Display
Display: 8.4 inch, TFT type color LCD with touch screen keys
Resolution: 800 × 600 dots
Screen size: approx. 170.4 × 127.8 mm
Display contents: Numerical data, scattergrams, histograms, measuring conditions, alarm message and other messages,
touch screen keys
Data Storage
Numerical data for all counted parameters for up to 400 samples and histograms and scattergrams for up to 60 samples
Environmental Conditions
Storage temperature: −20 to 60°C
Operating temperature: 15 to 30°C
Storage humidity: 10 to 95% (Non-condensing)
Operating humidity: 30 to 85% (Non-condensing)
Storage atmospheric pressure: 70 to 106 kPa
Operating atmospheric pressure: 70 to 106 kPa
Power Requirements
Power requirements: MEK-8222J: 110, 117 or 127 V ±10% AC, 50/60 Hz
MEK-8222K:220, 230 or 240 V ±10% AC, 50/60 Hz
Power consumption: 360 VA

1. GENERAL
Dimensions and Weight

Dimensions: 613 W × 550 D × 583 H (mm)
Net weight: approx. 55 kg
Electromagnetic Compatibility
IEC 61326-1 (1997) Amendment 1 (1998) Amendment 2 (2000)
EN 61326-1 (1997) Amendment 1 (1998)
CISPR11 (1997), Group 1, Class B

EN 55011 (1998) Amendment 1 (1999), Group 1, Class B
Safety
Safety standards: IEC 61010-1 2nd Edition (2001)
EN 61010-1 (1993) Amendment 2 (1995)
Laser: IEC 60825-1 (1993) Amendment 1 (1997)

EN 60825-1 (1994) Amendment 11 (1996)
According to the type of protection against electrical shock: CLASS I EQUIPMENT
According to the degree of protection against harmful ingress of water: IPX0 (Ordinary EQUIPMENT)
According to the degree of safety of application in the presence of a FLAMMABLE ANAESTHETIC MIXTURE WITH AIR,
OR WITH OXYGEN OR NITROUS OXIDE: EQUIPMENT not suitable for use in the presence of FLAMMABLE
ANAESTHETIC MIXTURE WITH AIR, OR WITH OXYGEN OR
NITROUS OXIDE
According to the mode of operation: CONTINUOUS OPERATION
EQUIPMENT types (classification): Indoor stationary EQUIPMENT
Installation Category: II EQUIPMENT
Pollution Degree: 2 EQUIPMENT
Requirements for marking of in vitro diagnostic instruments: EN1658 (1996)

1. GENERAL
Panel Descriptions
Front Panel 6 7 8 9 10
11
1
2 12
3 13
14
5
15
16 CAUTION
Do not put your hand in the
rack table during
measurement.
No. Name Description

1 Main power lamp Lights when the main power switch on the rear panel is turned on.
Turns the hematology analyzer power on or off when the main power switch on the rear panel
2 Power key is turned on. When the power is turned on, priming and self-check are automatically performed,
and the READY screen is displayed.
Stops operation when pressed during operation. Returns to the READY screen when pressed
3 Reset key while changing settings.
Use this key only when an error occurs.
Manual count For manual mode only.
4
switch Press to aspirate the sample and start counting.
Manual sampling For manual mode only.
5
nozzle Aspirates the sample.
Lights when the main power switch on the rear panel and power key on the front panel are
6 Power lamp
turned on.
7 Laser lamp Lights when the laser switch on the right side panel is turned on.
For manual mode only.
Operation indicator When blinking: Aspirating the sample
8
lamps When off: Counting the sample
When lit: Ready for next counting
Sets the measurement mode in manual mode when pressed. The sampling nozzle for manual
9 Manual mode key
mode is lowered.
10 LCD screen Displays measurement data, various messages and touch screen keys.
For rack mode only.
11 Eject key
Press this key to slide the sample rack in or out.
For rack mode only.
12 Start key
Press this key to start measurement.
13 Emergency key Press this key to count an emergency sample during routine counting.
Cleans the fluid path, aperture and manometer with detergent. Priming is performed
14 Clean key automatically after cleaning the fluid path. Press this key when clogging occurs, the manometer
becomes dirty or bubbles occur in the manometer.
15 Sample rack Holds the sample tubes containing specimens. Up to 50 sample tubes can be accommodated.
16 Rack table Holds the sample rack.

1. GENERAL
Right Side Panel 8
CAUTION 1
Do not insert an object, such a
pencil into these holes.
Otherwise the liquid sensor unit 2
may be damaged.
3
4
5
6
7
CAUTION
Always carry the hematology analyzer by more than two people and by
the left and right sides. Never hold it by the front and rear. Otherwise
the front panel may come off and the hematology analyzer may be
dropped, causing injury to the operator and damage to the hematology
analyzer.

Turns the laser on or off with the laser key for WBC 5 part
1 Laser switch
differential measurement.
ISO3
2 Inlets for the ISOTONAC•3 diluent.
Diluent inlets
CLN
3 Inlet for the CLEANAC detergent.
Detergent inlet
CLN3
4 Inlet for the CLEANAC•3 detergent.
Detergent inlet
HEMO3 or HEMO3N Inlet for the HEMOLYNAC•3 or HEMOLYNAC•3N hemolysing
5
Hemolysing reagent inlet reagent.
HEMO5
6 Inlet for the HEMOLYNAC•5 hemolysing reagent.
Hemolysing reagent inlet
WASTE Outlets for waste such as used lyse, detergent and aspirated
7
Waste outlets samples.
Flow cell unit window When two screws and the cover are removed, you can see the
8
cover flow cell unit. Open the window only when adjusting the gain.

1. GENERAL
Rear Panel
Refer to warnings and cautions in “Connecting an External
Instrument to the Hematology Analyzer” in Section 2.
5
6
Refer to warnings and cautions in “Connecting the Power 7

Cord and Grounding the Hematology Analyzer” in Section 2.

Connects to the optional WA-460V card printer, WA-711V
1 RS232C socket printer, WA-820V printer or PC. When a PC is connected to this
socket, histogram and scattergram data cannot be output.
2 Bar code socket Connects to the optional ZK-820V handy bar code reader.
3 USB socket Connects to a PC. Histogram and scattergram data can be output.
Supplies the power to the hematology analyzer when it is turned
4 Main power switch
on. Under normal conditions keep this switch turned on.
Contains the time lag fuse. To replace the fuse, contact your
5 Fuse holder
Nihon Kohden distributor.
AC SOURCE Connects the AC power cord to supply AC power to the
6
AC source socket hematology analyzer.
Equipotential ground Connects the ground lead to the equipotential ground terminal on
7
terminal the wall for earth grounding.

1. GENERAL
Inside Panels
CAUTION
• For WBC 5 part differential measurement, the laser beam is used. Do
not open any part labeled “CAUTION”. The laser can cause burns
and blindness.
• Do not remove the caution labels.
NOTE
• When attaching the filter joint assembly, be careful not to bend or
damage the filter packing at the bottom of the measurement bath.
• When there is a leakage, check that there is no scratch or damage to
the circumference of the filter.

1. GENERAL
Composition
MEK-8222J JQ-820V Inlet/Outlet Unit

MEK-8222K
XP-513V 3-way Valve
JQ-821V Left Side Piping Unit
XP-513V 3-way Valve
JQ-822V Center Piping Unit
UT-7171 LIQUID LEVEL SENSOR Board
XP-513V 3-way Valve
JQ-823V 4-channel Liquid Sensor Unit
UT-7179 4WAY LIQUID SENSOR Board
MB-820V Sub Bath Unit
MC-820V CBC Measuring Unit
UT-7143 PRE AMP Board
UT-7180 CBC Measuring Board Set
UT-7181* HGB AMP Board

* Not available
UT-7182* HGB LED Board
UT-7183* MANOMETER PTR Board
UT-7184* MANOMETER LED Board
XP-512V 2-way Valve
XP-513V 3-way Valve
MC-821V Flow Cytometry Piping Unit
UT-7152 DRIVER1 Board
XP-512V 2-way Valve
XP-513V 3-way Valve

1. GENERAL
MO-820V Laser Optical Unit
MF-820V Flow Cell Unit
UT-7172 MO Preamp Board Set
UT-7173 MO FS PREAMP Board
UT-7174 MO FL PREAMP Board
UT-7175 MO SD PREAMP Board
UT-7176 MO LD DRIVER Board
MP-520V Pump Unit
MP-820V Complex Pump Unit
UT-7177 MD/MP Connection Board Set
UT-7186 MD CONNECTION Board
UT-7187 MP CONNECTION Board
XP-503V 3-way Valve
XP-513V 3-way Valve
MP-821V Sample Pump Unit
XP-512V 2-way Valve
MP-822V Sheath Pump Unit
UT-7158 SHEATH CONTROL Board
UT-7171 LIQUID LEVEL SENSOR Board
XP-512V 2-way Valve
MS-820V Closed Sampler Unit
UT-7185 3POS SENSOR Board
XP-513V 3-way Valve
MS-821V Open Sampler Unit
UT-7065 SENSOR Board

1. GENERAL
MS-822V Table Unit
UT-7086 SENSOR3 Board
UT-7157 TABLE SENSOR Board
PV-820V Display Unit
UT-7178 Front Panel Board Set
* Not available UT-7149* LCD CONTROL Board
UT-7150* MANUAL KEY Board
UT-7151* RACK KEY Board
RK-820V Chassis
SC-820VJ Power Supply Unit

SC-820VK
UT-7146 MASTER Board
UT-7147 SLAVE CBC Board
UT-7148 SLAVE MS Board
UT-7160 POWER Board
UT-7161 INTERFACE Board
ZY-820V Warmer Unit
UT-7159 WARMER CONTROL Board
XP-513V 3-way Valve

1. GENERAL
Demonstration Guide
Refer to Section 9 for an installation and setup flow chart for a demonstoration of
the MEK-8222J/K hematorogy analyzer.

1. GENERAL
How to Upgrade the Software
Instruments with the software version V01-07 require the following ROM
replacement before the software upgrade. Therefore, check the software
version of the instrument. If the software version is not V01-07, go to
“Upgrading the Software with Memory Card”.
Replacing the ROM

1. Turn off the main power switch on the rear panel of the instrument and unplug
the AC power cord.
CAUTION
Before removing any parts from the instrument, wait 10 minutes after
turning off the instrument and disconnecting the power cord from the
AC outlet.
2. Remove the screws which secure the side covers and top cover to the
instrument chassis. Remove the covers from the instrument.
Top cover
Left side cover
Right side cover
Unplug the power cord
Rear cover

1. GENERAL
3. Remove the screws which secure the rear cover to the instrument chassis.
Slowly pull the rear cover toward you until the cable connected to the rear
cover backside appears as shown below. Disconnect the cable at the
INTERFACE board which is attached to the rear cover. Completely remove the
rear cover from the instrument.
4. Insert a small flat-blade screwdriver between the ROM and its socket on the
MASTER board as shown below. Carefully lift the ROM with the screwdriver
until the ROM slightly tilts. Take out the screwdriver and insert it into the
opposite side of the IC socket. Continue lifting both sides of the ROM with the
screwdriver until the ROM is removed.
MASTER board
Flat-blade screwdriver
ROM

1. GENERAL
CAUTION
When you remove the ROM from the IC socket with a sharp edge tool
such as a flat-blade screwdriver, be sure that it does not damage the
printed pattern under the IC socket.
IC socket
Printed pattern
5. Put the new ROM on the IC socket and carefully insert the pins of the new
ROM into the IC socket.
CAUTION
• Before putting the new ROM on the IC socket, check that the ROM
position is the same as the old ROM.
• Do not break or bend a pin of the ROM. The pins must be correctly
inserted into the socket. Otherwise the instrument may not work.

1. GENERAL
Upgrading the Software with the Memory Card

1. Turn off the main power switch on the rear of the instrument.
2. Remove the right side cover as shown below.
Right side cover

Main power switch
3. Insert the memory card into the slot as shown below.
Memory card
Card slot
4. Turn on the main power switch on the rear of the instrument.

1. GENERAL
5. Turn on the Power key on the front panel of the instrument.

The “DOWNLOAD MEK-8222 PROGRAM” screen appears.
The MEK-8222 software has five parts.
MASTER BD (board)
SLAVE CBC BD (board)
SLAVE MS BD (board)
LANGUAGE: ENGLISH (local language program)
ANALYSIS PROGRAM
6. Install the software as follows:

To install all the software, press the DOWNLOAD ALL PROGRAM
touch screen key.
To install the MASTER BD software only, press the MASTER BD Vxx-
xx (xxxx) key on the screen.
To install the SLAVE CBC BD software only, press the SLAVE CBC BD
Vxx-xx (xxxx) key on the screen.
To install the SLAVE MS BD software only, press the SLAVE MS BD
Vxx-xx (xxxx) key on the screen.
To install the LANGUAGE software, press the LANGUAGE (local
language ) Vxx-xx (xxxx) key on the screen.
To install the ANALYSYS PROGRAM software only, press the
ANALYSYS PROGRAM Vxx-xx (xxxx) key on the screen.

1. GENERAL
After the software is installed, the following screen appears.
7. Press the OK key on the screen.
The instrument starts automatic priming.
8. Press the card eject key and remove the memory card from the instrument.
Memory card
Card eject key
9. Reassemble the instrument completely.

Section 2 Troubleshooting
Check Procedure Flowchart ................................................................................................ 2.1

Operating Condition and Blood Sample Checks .................................................................. 2.2
Operating Condition Check ........................................................................................ 2.2
Blood Sample Handling Check .................................................................................. 2.2
Capillary Sample Preparation Check ......................................................................... 2.2
Atypical Blood Samples ............................................................................................ 2.3
Checking Particle CV .......................................................................................................... 2.8
Measuring Polymer Microsphere Suspensions for Checking CV ............................... 2.8
Poor Optical Reproducibility Problems .................................................................... 2.10
No Scattergram and Histograms (TOC (Total Optical Count) is 0) ................. 2.10
Scattergrams and Histograms Appear But Low TOC (Below 2000) ............... 2.10
CV of FS or FL is Above 7% ........................................................................ 2.11
Adjusting the Flow Cell Unit Position ....................................................................... 2.11
Checking Background Noise ............................................................................................. 2.13
Measurement Procedure ......................................................................................... 2.13
Measurement ................................................................................................ 2.13
Results .......................................................................................................... 2.13
Background Noise Problems ................................................................................... 2.14
Checking the Reproducibility ............................................................................................. 2.15
About CV Values ..................................................................................................... 2.15
Checking Procedure ................................................................................................ 2.16
Poor Reproducibility Problems ................................................................................ 2.16
Poor WBC Reproducibility .............................................................................. 2.16
Poor WBC 5 Part Differential Reproducibility ................................................. 2.18
Poor HGB Reproducibility .............................................................................. 2.19
Poor RBC Reproducibility .............................................................................. 2.20
Poor PLT Reproducibility ............................................................................... 2.21
Poor HCT or MCV Reproducibility ................................................................. 2.23
Checking Accuracy ........................................................................................................... 2.24
Changing the Operator to “FACTORY” ..................................................................... 2.24
Calibration Procedure .............................................................................................. 2.24
Checking Scattergram ....................................................................................................... 2.26
Checking Scattergram Procedure ............................................................................ 2.26

Scattergram Problems ............................................................................................ 2.28
Differential Parameters Rough Gain Problems .............................................. 2.28
Differential Parameters Fine Gain Problems ................................................. 2.28
Solving Problems from Error Messages ............................................................................ 2.30
Error Messages ....................................................................................................... 2.30
A001: No diluent (type 1) ............................................................................... 2.30
A002: No diluent (type 2) ............................................................................... 2.30
A003: No diluent (flowcyte type 1) ................................................................. 2.31
A004: No diluent (flowcyte type 2) ................................................................. 2.31
A005: No detergent (CLEANAC) .................................................................... 2.32
A006: No detergent (CLEANAC•3) ................................................................ 2.32
A007: No hemolysing reagent (HEMOLYNAC•3) ........................................... 2.32
A008: No hemolysing reagent (HEMOLYNAC•5) ........................................... 2.33
A009: WBC priming error ............................................................................... 2.33
A010: RBC priming error ................................................................................ 2.33
A021: WBC fluid level 1 ................................................................................. 2.34
A022: WBC fluid level 2 ................................................................................. 2.34
A023: WBC fluid level 3 ................................................................................. 2.34
A024: WBC bubble 1 ..................................................................................... 2.35
A025: WBC bubble 2 ..................................................................................... 2.35
A026: WBC bubble 3 ..................................................................................... 2.35
A027: WBC bubble 4 ..................................................................................... 2.35
A028: WBC manomtr dirty ............................................................................. 2.35
A029: WBC clogged ...................................................................................... 2.36
A030: WBC sample error ............................................................................... 2.36
A031: WBC hardware noise ........................................................................... 2.36
A032: WBC software noise ............................................................................ 2.36
A041: RBC fluid level 1 ................................................................................. 2.37
A042: RBC fluid level 2 ................................................................................. 2.37
A043: RBC fluid level 3 ................................................................................. 2.37
A044: RBC bubble 1 ...................................................................................... 2.38
A045: RBC bubble 2 ...................................................................................... 2.38
A046: RBC bubble 3 ...................................................................................... 2.38
A047: RBC bubble 4 ...................................................................................... 2.38
A048: RBC manomtr dirty ............................................................................. 2.38
A049: RBC clogged ....................................................................................... 2.39
A050: RBC sample error ................................................................................ 2.39
A051: RBC hardware noise ............................................................................ 2.39
A052: RBC software noise ............................................................................ 2.39
A061: HGB voltage low ................................................................................. 2.40
A062: HGB voltage high ................................................................................ 2.40
A063: HGB circuit error ................................................................................. 2.40
A071: Mixing error ......................................................................................... 2.40
A081: Laser switch off ................................................................................... 2.41
A082: Optical count error .............................................................................. 2.41
System Error Messages .......................................................................................... 2.42
E032: Circuit error ......................................................................................... 2.42
E033: Check settings .................................................................................... 2.42
E101: CLOSED SAMPLER ERROR ............................................................. 2.42
2C.2 Service Manual MEK-8222

E102: OPEN SAMPLER ERROR .................................................................. 2.42
E103: TABLE ERROR ................................................................................... 2.42
E104: MIXING ERROR ................................................................................. 2.42
E105: BARCODE ERROR ............................................................................. 2.43
E106: DILUTER ERROR ............................................................................... 2.43
E107: LYSE PUMP ERROR .......................................................................... 2.43
E108: SAMPLE PUMP ERROR .................................................................... 2.43
E109: SHEATH PUMP ERROR ..................................................................... 2.43
E110: 5DIFF ROTARY PUMP ERROR .......................................................... 2.43
E111: WBC ROTARY PUMP ERROR ............................................................ 2.44
E112: RBC ROTARY PUMP ERROR ............................................................. 2.44
E113: WBC SUB BATH ERROR .................................................................... 2.44
E114: RBC SUB BATH ERROR .................................................................... 2.44
E115: WARMER ERROR ............................................................................... 2.44
E201: MASTER BD ERROR ......................................................................... 2.44
E202: SLAVE MS BD ERROR ...................................................................... 2.44
E203: SLAVE CBC BD ERROR .................................................................... 2.45
E301: MASTER-MS COMM ERROR ............................................................ 2.45
E302: MASTER-CBC1 COMM ERROR ......................................................... 2.45
E303: MASTER-CBC2 COMM ERROR ......................................................... 2.45
E304: MS-CBC COMM ERROR .................................................................... 2.45
E305: MS-BARCODE COMM ERROR .......................................................... 2.45
Maintenance Information ......................................................................................... 2.46
Solving Problems from Flags ............................................................................................ 2.47
Blasts and Immature Granulocyte ................................................................. 2.47
Left Shift ....................................................................................................... 2.47
Atypical Lymphocytes ................................................................................... 2.48
! appears on the right of WBC measured value ............................................. 2.48
! appears on the right of MCHC measured value ........................................... 2.49
C appears on the right of WBC or PLT measured value ................................. 2.49
Service Maintenance Screens ........................................................................................... 2.51
Displaying the SERVICE MAINTENANCE Screen .................................................. 2.51
CHECK & MAINTENANCE Screen ......................................................................... 2.52
MEASURE WBC & RBC ............................................................................... 2.52
MEASURE WBC ........................................................................................... 2.52
MEASURE RBC ........................................................................................... 2.52
CIRCUIT CHECK .......................................................................................... 2.53
PRIME BATHS .............................................................................................. 2.53
UNIT CLEAN ................................................................................................. 2.53
DRAIN BATHS .............................................................................................. 2.53
REMOVE FLOWCELLS ................................................................................ 2.53
LIQUID TANK PRIME .................................................................................... 2.53
BACKUP RAM CHECK ................................................................................. 2.53
SAMPLE UNIT MAINTENANCE Screen ................................................................. 2.54
MS-820V ....................................................................................................... 2.54
MS-821V ....................................................................................................... 2.55
MS-822V ....................................................................................................... 2.55
CLEANING NEEDLE..................................................................................... 2.56

PUMP UNIT MAINTENANCE Screen ..................................................................... 2.56
MP-520V 5DIFF, MP-520V WBC, MP-520V RBC .......................................... 2.56
MP-820V ....................................................................................................... 2.57
MP-821V ....................................................................................................... 2.58
VALVE & PUMP CHECK .............................................................................. 2.59
CONTINUOUS MEASUREMENT Screen ................................................................ 2.60
CLOSED MODE 10 SAMPLES ..................................................................... 2.60
CLOSED MODE 30 SAMPLES ..................................................................... 2.60
RACK 1, 2 40 SAMPLES .............................................................................. 2.60
MANUAL MODE CONT MEAS. .................................................................... 2.60
CLOSED MODE CONT MEAS. .................................................................... 2.60
MONITOR Screen ................................................................................................... 2.61
SENSOR MONITOR ..................................................................................... 2.61
SCI MONITOR .............................................................................................. 2.62
POS SENSOR MONITOR ............................................................................. 2.62
10 DATA X-CV Screen ............................................................................................. 2.63
INITIALIZATION MENU Screen .............................................................................. 2.63
ADVANCED SETTINGS Screen .............................................................................. 2.64
MEASUREMENT MODE .............................................................................. 2.65
UNIT SETTINGS .......................................................................................... 2.66
SCREEN SETTINGS .................................................................................... 2.67
FLAGS SETTINGS ....................................................................................... 2.67
MAINTENANCE ALARM ............................................................................... 2.69
Modification Procedure for Other Sample Tubes ................................................................ 2.70
Optional Racks for S-Monovette and KABE sample tubes ...................................... 2.73

2. TROUBLESHOOTING
Check Procedure Flowchart

Check the hematology analyzer according to the following check procedure flowchart.
Are the following conditions acceptable?
- Reagents No
Refer to “Operating Condition and Blood Sample Checks” section.
- Temperature
- Blood sample
Yes
Are there no error messages such as the following on the screen

when the power is turned on?
- No diluent
No
- No detergent Refer to “Solving Problems from Error Messages”
- No hemolysing reagent section.
- xxx … initialize error
- xxx priming error
Yes
Check the following after the particle measurement.

- Is TOC (total optical count) acceptable? No
Refer to “Checking Particle CV” and “Adjusting
- Do the CV values of FS and FL meet the following conditions?
the Flow Cell Unit Position” sections.
FS: ≤7% FL: ≤7%
Yes
Check the specified parameters after the background noise

measurement. No
Refer to “Checking Background Noise” section,
- Are the WBC, RBC, HGB, PLT and TOC data acceptable?
“Background Noise Problems” table and “Solving
- Is there no error message?
Problems from Error Messages” section.
Yes
Check the following after the hematology control MEK-3D measurement.

- Is there no error message?
- Are the following parameter reproducibility acceptable? No
Refer to “Solving Problems from Error
PLT, HGB, Parameters related to WBC and PLT, HCT and MCV
Messages” and “Checking Accuracy”
- Are all the obtained parameters within the acceptable range on the assay
sections.
sheet?
Yes
Check the following after the hematology control MEK-5D measurement.

No
- Are the 5-part differential WBC data reproducibility acceptable? Refer to “Checking the Reproducibility”
- Are the obtained scattergrams acceptable? and “Checking Scattergram” sections.
Yes
Check the following after healthy human blood sample measurement.

No
- Are the obtained scattergrams acceptable? Refer to “Checking Scattergram”.
- Is there no flag?
Yes
END

2. TROUBLESHOOTING
Operating Condition and Blood Sample Checks
Operating Condition Check The measurement requires the following operating conditions.
Operating temperature: 15 to 30°C

Operating humidity: 30 to 85%
Operating atmospheric pressure: 70 to 106 kPa
If the diluent or hemolysing reagent temperature is lower than 15°C, it will affect the
measurement data of HGB, WBC and WBC 5 part differential parameters or cause the
hemolysing error flag or sample error alarm.
- Check that the blood sample is immediately counted after collection or stored in
Blood Sample Handling
a cool place (at temperatures between 2 and 8°C), such as a refrigerator. If the
Check
blood sample is stored in a refrigerator at temperatures lower than 2°C for a long
time or left more than 12 hours at room temperature after collection, the WBC 5
part differential parameters may be affected.
- If there is a blood sample which causes hemolysing error flag when it is counted
within 30 minutes after collection, check that the blood sample is left at least 30
minutes after collection before counting it.
- When counting a blood sample which is left more than a few minutes after
collection, check that the blood sample is gently shaken again before counting.
- Check that there are no bubbles in the blood sample after shaking it. If the blood
sample is excessively shaken, it causes a lot of bubbles and hemolyzation.
- Check that an aggregated or coagulated blood sample is not counted. Such a blood
sample may damage the hematology analyzer.
- When counting a blood sample which is stored in a refrigerator for more than a
day, check that the sample is warmed to room temperature and gently and
sufficiently shaken for counting.
Capillary Sample Most of the measurement data error in the capillary mode is caused by blood collection
Preparation Check and dilution of the blood sample. Therefore, check the following points. Refer to
“Measuring a Capillary Sample” in Section 5 of the MEK-8222J/K operator’s manual.
- Before using a sahli pipette for capillary blood collection, check that the sahli
pipette is completely cleaned and dried. An optional micro cap is recommended
for capillary blood collection. Refer to “Consumables” in Section 11 of MEK-
8222J/K operator’s manual.
- When putting the capillary blood into a sample cup, check that there are no bubbles
in the sample cup. If the user cannot avoid making bubbles with the sahli pipette,
we recommend the optional micro cap which prevents bubbling.
- Check that the capillary blood in the sample cup is properly aspirated by the
sampling nozzle after gently shaking the sample cup more than 10 times. If a
venous blood is erroneously aspirated, the hematology analyzer can have a
malfunction such as clog in the fluid path or high background noise.

2. TROUBLESHOOTING
Atypical Blood Samples The following atypical blood samples can affect the specified parameter(s).
WBC:
Excessive WBC
If the white blood cells are too high to count, use the HIGH WBC key in the manual
mode. If the hematology analyzer still displays the OVER message, dilute the blood
sample so that the WBC is within the measurable range.
Nucleated erythrocyte
If there is a nucleus in some of the red blood cells, the nucleated red blood cells are
detected as WBC which increases the WBC count. Subtract the number of nucleated
red blood cells from the total WBC count.
Unlysed red blood cells

In some rare occasions, the RBC in the blood sample may not completely lyse and
these non-lysed RBC may be detected as WBC and cause increase in WBC count.
Multiple myeloma
The precipitation of proteins in multiple myeloma patients may increase the WBC
count.
Leukemia
Since WBC in leukemia patients is fragile, some of the WBC may be destroyed during
measurement. It may cause low WBC count. The fragments of the WBC may also
affect the accuracy of the WBC 5 part differential measurement.
Chemotherapy
Cytotoxic or immunosuppressive drugs weaken WBC so that some of the WBC may
be destroyed during measurement. It may cause low WBC count. The fragments of the
WBC may also affect the accuracy of the WBC 5 part differential measurement.
Cryoglobulins
Increase of cryoglobulin may be caused by myeloma, cancer, leukemia,
macroglobulinemia, lymphoproliferative disorders, metastatic tumors, autoimmune
disorders, infections, aneurysm, pregnant, thromboembolic phenomena or diabetes.
The increase of cryoglobulin causes increase in WBC, RBC, PLT counts and HGB
concentration. To remove this effect from the blood sample, warm the blood sample to
37°C in a water bath for 30 minutes and measure the sample immediately.

2. TROUBLESHOOTING
RBC:
Leukemia
An increase in WBC in leukemia patients causes increase in RBC because the WBC
count is so high that it affects RBC count. If the WBC count is 50 x 103/µL or more,
subtract the WBC count from the RBC count to remove this effect.
Agglutinated RBC
Agglutinated RBC may decrease RBC count. It is found by checking the MCH and
MCHC values because they become abnormal if the low RBC count is caused by
agglutinated RBC. When you gently tilt the blood sample tube and watch the blood on
the tube wall, you can see if the blood is granular. If using the stained blood film and
checking it with a microscope, you can check if the sample has agglutinated RBC.
Cold agglutinins
IgM immunoglobulin increases in cold agglutinin disease patient. The blood sample
of the patient may decrease the RBC and PLT counts and increase the MCV. To remove
this effect from the blood sample, warm the blood sample to 37°C in a water bath and
measure the sample immediately. If the cold agglutinins are too high, the stained blood
film may show that the blood looks coagulated.
Hemolysis
If some of the red blood cells are hemolysed, the blood sample may cause low RBC.
HGB:
Turbidity of the blood sample
Physiologic, therapeutic or disease factors may cause turbidity of the blood sample.
Turbidity affects the HGB concentration. Find the cause of turbidity and follow the
appropriate method for accurate HGB concentration as shown below.
- High WBC
Increase of WBC causes turbidity. It can cause excessive light scatter and incorrect
HGB. Manually measure the HGB. Centrifuge the diluted sample and measure the
supernatant fluid with a spectrophotometer.
- Increase of lipids
The blood sample may be milky when there is excessive lipid. It can be caused by
hyperlipidemia, hyperproteinemia or hyperbilirubinemia. Manually measure the HGB
and use the plasma blank measurement to obtain the accurate HGB.
- Increase of unlysed RBC
When RBC which have resistance to lysing are not lysed by the hemolysing reagent,
turbidity increases. It causes high HGB. Since HGB affects MCH and MCHC, the
MCH and MCHC show abnormal values.
- Fetal blood
The mixing of fetal and maternal blood may increase the HGB.
- Excessive WBC
If there is a blood sample which shows abnormally high WBC, it increases turbidity
and high HGB is shown. The MCH and MCHC are also high.

2. TROUBLESHOOTING
HCT:
Agglutinated RBC
RBC agglutination may cause erroneous HCT and MCV values. The stained blood
film shows these errors. If the MCH and MCHC values are also abnormal, manually
measure the HCT and MCV.
MCV:
Agglutinated RBC
RBC agglutination may cause erroneous HCT and MCV values. The stained blood
film shows these errors. If the MCH and MCHC values are also abnormal, manually
measure the HCT and MCV.

Excessive number of large PLT or excessively high WBC may affect the MCV value.
Use the stained blood film to obtain the MCV value.
MCH:
MCH is calculated from HGB and RBC values. Therefore, the abnormal HGB or
RBC affects the MCH.
MCHC:
MCHC is calculated from HGB and HCT values. Therefore, the abnormal HGB or
HCT affects the MCHC.
RDW:
RDW is calculated from RBC value. Therefore, the abnormal RBC affects the RDW.
Agglutinated RBC
Agglutinated RBC may decrease RBC count and erroneous RDW. This can be checked
by abnormal MCH and MCHC values and examination of the stained blood film.

PLT:
Very small RBC, RBC fragments or WBC fragments can cause the increase of the PLT
count.
Agglutinated RBC
PLT can be involved in the agglutinated RBC. It decreases the PLT count. Abnormal
MCH and MCHC values shows this occurrence. Use the stained blood film to obtain
the PLT.
Very large PLT

If there are large PLT, such as Bernard-Soulier syndrome, which exceed the PLT
threshold, the large PLT are not counted as PLT and the PLT count decreases.

2. TROUBLESHOOTING
Chemotherapy
Cytotoxic or immunosuppressive drugs weaken the cells. It can cause low PLT count.
Manually measure the PLT.
Hemolysis
Lyzed specimen contains red blood cell stroma. It can increase the PLT count.
Blood anticoagulated with acid-citrate-dextrose causes agglutinated PLT. It can decrease
the PLT count.
Agglutinated PLT
Agglutinated PLT decreases the PLT count and increases the WBC count. For such
sample, collect the sample in sodium citrate anticoagulant and measure only PLT. The
PLT must be corrected with the sodium citrate dilution effect to obtain the accurate
PLT.
MPV:
Very large PLT
Large PLT exceeds the PLT threshold and is not counted as PLT. It results in low
MPV.

Very small RBC, RBC fragments and WBC fragments can affect the MPV measurement.
Agglutinated RBC
PLT can be included in the agglutinated RBC. It results in erroneous MPV. Abnormal
MCH and MCHC values show this occurrence. Use the stained blood film to obtain
the PLT.
Chemotherapy
Cytotoxic or immunosuppressive drugs weaken the cells. It can cause erroneous MPV.
Manually measure the MPV.
NOTE
- Swelling of platelets depends on the storage temperature and elapsed
time after collection.
- Blood samples collected in EDTA can cause unstable MPV.

2. TROUBLESHOOTING
NE% and NE count:

Excessive eosinophils, metamyelocytes, myelocytes, promyelocytes, blasts and plasma
cells can affect the accuracy of the NE% and NE count.
LY% and LY count:

Erythroblasts (NRBC), certain parasites and unlysed RBC that have resistance to lysis
can affect the accuracy of the LY% and LY count.
MO% and MO count:

Large lymphocytes, atypical lymphocytes, blasts and excessive number of basophils
can affect the accuracy of the MO% and MO count.
EO% and EO count:

Abnormal granules can affect the accuracy of the EO% and EO count.
BA% and BA count:

Immature cell, metamyelocytes, myelocytes, promyelocytes, blasts and plasma cells
can affect the accuracy of the BA% and BA count.
NOTE
Cell counts for WBC 5 part differential parameters, NE, LY, MO, EO and
BA counts, are calculated from the WBC count (by electrical resistance
detection) and each percentage (by laser beam scatter). Therefore, the
WBC count can affect these parameters.

2. TROUBLESHOOTING
Checking Particle CV
Measuring Polymer Checking CV of particle measurement is necessary for accurate WBC 5 part
Microsphere Suspensions differential measurement. Check the particle CV after installing the hematology
for Checking CV analyzer or when the scattergrams appear outside their allotted area on the screen.
To check particle CV, measure 7 µm polymer microsphere suspensions.
1. Prepare the 7 µm polymer microsphere suspensions (supply code no. T905) in

a sample tube.
2. Press the SETTINGS key on the MENU screen to display the SETTINGS
screen.
3. Press the SENSITIVITY THRESHOLD key on the SETTINGS screen to

display the SENS & THRESH screen.
4. Press the DIFF GAIN key to display the DIFF GAIN (ROUGH) screen.
The ideal peak and current gain values are displayed on the screen.
5. Press the Manual mode key on the front panel to enter manual mode, put the
sampling nozzle into the bottom of the sample tube containing the polymer
microsphere suspensions so that the tip of the sampling nozzle touches the
bottom of the sample tube, and press the Manual count switch. The polymer
microsphere suspensions is aspirated and measurement starts.
The result appears on the screen. Check that the CV of FS and FL are below
7%.

2. TROUBLESHOOTING
CV values
When the result is not optimum, press the CLEAN FLOW CELLS key to clean
the flow cell unit. After cleaning, measure the polymer microsphere
suspensions again.
When the result is still not optimum, the flow cell unit position must be
adjusted. Refer to the “Adjusting the Flow Cell Unit Position” section.
6. Press the OK key on the screen to return to the SENS & THRESH screen.
Cleaning is automatically performed.

2. TROUBLESHOOTING
Poor Optical
Reproducibility Problems No Scattergram and Histograms (TOC (Total Optical Count) is 0)
Reason Possible Cause/Criteria Countermeasure
The laser switch on the right panel is

Turn on the laser switch.
turned off.
No laser light. The emergency switch* is turned off. Turn on the switch.
The MO-820V Laser Optical Unit
Connect the unit.
connector is disconnected.
MO-820V Laser Optical Unit failure. Replace the unit with a new one. Refer to Section 4.
UT-7146 MASTER board failure. Replace the board with a new one. Refer to Section 4.
Adjust the flow cell unit position. Refer to the
Incorrect flow cell unit position.
“Adjusting the Flow Cell Unit Position” section.
Replace the pinch valve tubes with new ones. Refer
The pinch valve tubes are clogged.
to Section 4.
MP-821V Sample Pump Unit failure. Replace the unit with a new one. Refer to Section 4.
No sample flow into
MP-822V Sheath Pump Unit failure. Replace the unit with a new one. Refer to Section 4.
the flow cell.
Contact your Nihon Kohden distributor.
failure.
Set FS THR to 10 on the DIFF GAIN (FINE) screen.
The FS THR setting of the diff gain is
No waveform is Refer to “Adjusting Gain for WBC 5 Part Differential
inappropriate.
detected. Measurement” in Section 3 of the operator’s manual.
*Located near the laser switch on the chassis. Refer to Section 8 “Replaceable Parts List” pages 8.6 and 8.7. When this emergency switch
is turned on, the LED of the switch is lit. Without removing the top cover, you can turn on the switch using a thin stick such as a tooth pick
through the ventilation grille.
Scattergrams and Histograms Appear But Low TOC (Below 2000)

Replace the polymer microsphere suspensions with a
Poor quality polymer microsphere suspensions.
new one.
The pinch valve tubes are clogged. Replace the pinch valve tubes with new ones.
MP-520V Pump Unit failure. Replace the unit with a new one. Refer to Section 4.
The flow of the
sample is unstable. MC-821V Flow Cytometry Piping Unit
failure.
inappropriate.

2. TROUBLESHOOTING
CV of FS or FL is Above 7%
The flow cell unit position is inappropriate.
Replace the polymer microsphere suspensions with
The quality of the polymer microsphere suspensions is poor.
the new one.
Inside of the flow cell unit is dirty.
Clean the flow cell unit. Refer to “Cleaning the Flow
Air bubbles on the inside wall of the flow Cell” in Section 10 of the operator’s manual.
cell.
The flow of the
The MP-822V Sheath Pump Unit failure. Replace the unit with a new one. Refer to Section 4.
sample is unstable.
The flow cell unit is clogged. Contact your Nihon Kohden distributor.
The MC-821V Flow Cytometry Piping
Unit failure.
The outside of the flow cell unit is dirty. Contact your Nihon Kohden distributor.
The light scatter is
The flow cell unit is damaged. Replace the flow cell unit with a new one.
unstable.
The MO-820V Laser Optical Unit failure. Replace the unit with a new one.
Adjusting the Flow Cell Adjust the flow cell unit position when the CV of FS or FL is still above 7% after
Unit Position cleaning the flow cell unit.
1. Remove the flow cell unit window cover on the right side panel by removing
two screws.
Flow cell unit window cover
2. Loosen the screw fastening the flow cell unit adjustment screw with the thick
hex wrench. Turn the screw counterclockwise.
Hex wrench (thick type)
Flow cell unit adjustment screw 3. Insert the thin hex wrench into the flow cell adjustment screw.
Hex wrench (thin type)

2. TROUBLESHOOTING
4. Prepare the polymer microsphere suspensions in a sample tube and measure it

by referring to steps 2 to 5 in the “Measuring Polymer Microsphere
Suspensions for Checking CV” section.
5. When the measurement is started, turn the flow cell adjustment screw about 2
or 3° and press the CLEAR key on the screen to redraw the histograms.
The polymer microsphere suspensions measurement lasts for about 25 seconds.

If adjusting the flow cell unit is not complete within this period, measure
polymer microsphere suspensions again.
If the histograms are narrower than before, turn the flow cell adjustment screw
in the same direction.
If the histograms are wider than before, turn the flow cell adjustment screw in
the opposite direction.
Repeat drawing the histograms, adjusting the flow cell unit and redrawing the
histograms until you obtain the optimum result (narrowest histograms width).
6. When adjusting the flow cell unit position is complete, fasten the flow cell unit
adjustment screw with the thick hex wrench. Turn the screw clockwise.
7. Measure polymer microsphere suspensions again and check that the CV values
are below 7%.
8. Replace the flow cell unit window and press the OK key on the screen.

2. TROUBLESHOOTING
Checking Background Noise
Measurement Procedure Measure the diluent to check the background noise.
Measurement
Count the diluent in closed mode and manual mode.
In Closed Mode
1. Press the Eject key on the front panel. The rack table slides out.
2. Set a few empty sample tubes in the rack.
3. Press the Start key on the front panel to count the empty samples.
In Manual Mode
1. Press the Manual mode key on the front panel. The sampling nozzle is
lowered.
2. Press the Manual count switch to count the diluent. There is no need to
aspirate the diluent from the sampling nozzle.
Results
The result is displayed on the screen after measurement. Make sure that the values
are less than or equal to the following values.
WBC: 0.2 (×103/µL)
RBC: 0.05 (×106/µL)
HGB: 0.1 (g/dL)
PLT: 10 (×103/µL)
TOC: 100 (count)
(TOC: total optical count. This parameter only appears in a background noise
measurement.)
Disregard the other parameter values because noise does not affect the other
parameters.

2. TROUBLESHOOTING
Background Noise Problems
Problem Possible Cause/Criteria Countermeasure

Clean the diluent container or replace the diluent
The diluent or diluent container is dirty.
with a new one.
The fluid paths inside the hematology Press the Clean key on the front panel to perform
analyzer are dirty. cleaning.
High background Connect the hematology analyzer power cord to
noise of all another AC outlet. If possible, use an independent
Noise interference through AC source or
parameters. AC outlet only for the hematology analyzer.
other instruments nearby.
Perform equipotential grounding and remove the
other instruments if they are the cause of noise.
The reagents are not used in the appropriate
Use the reagents at the appropriate temperature.
temperature range or they are deteriorated.
Clean the apertures or replace the aperture caps with
The apertures are dirty.
new ones.
The sampling nozzle is dirty. Clean the sampling nozzle.
The measurements baths and sub baths are Clean the measurement baths and sub baths or
dirty. replace them with new ones.
High background
The apertures are damaged. Replace the aperture caps with new ones.
noise of WBC, RBC
and PLT. MC-820V CBC Measuring Unit failure. Replace the unit with a new one.
The sampling nozzle is clogged. Replace the sampling nozzle with a new one.
MP-820V Complex Pump Unit failure. Replace the unit with a new one.
UT-7147 SLAVE CBC Board failure. Replace the board with a new one.
UT-7160 POWER Board failure. Replace the board with a new one.
The measurements baths and sub baths are Clean the measurement baths and sub baths or
dirty. replace them with new ones.
MC-820V CBC Measuring Unit failure. Replace the unit with a new one.
High background The sampling nozzle is clogged. Replace the sampling nozzle with a new one.
noise of HGB.
Clean the flow cells by pressing CLEAN FLOW
The flow cells are dirty.
CELLS on the OTHER OPERATION screen.
The sample cup is dirty. Clean the sample cup.
High background MF-820V Flow Cell Unit failure. Replace the unit with a new one.
noise of TOC.
MO-820V Laser Optical Unit failure. Replace the unit with a new one.
UT-7146 MASTER Board failure. Replace the board with a new one.

2. TROUBLESHOOTING
Checking the Reproducibility
The CV values of the 10 measurements of the same type of blood are used for
checking the reproducibility. The MEK-3DN hematology control, MEK-5DN
hematology control or human blood of a healthy person which is within 8 hours
after collection can be used. If the CV values are out of the specified range, the
hematology analyzer does not operate normally. When the reproducibility is poor,
refer to the “Poor Reproducibility Problems” section and solve the problems.
About CV Values The reproducibility is checked using the CV values. The CV value is acquired by
dividing the standard deviation by the average value. When the CV value is large,
it indicates that the measurement data variability is large (i.e. reproducibility is
poor).
Example
Measured Value Acquired CV Standard CV
Parameters
(average) (%) (%)
WBC 6.8 1.3 2.0
NE% 5.0
LY% 5.0
MO% 12.0
EO% 20.0
BA% 30.0
RBC 4.43 0.8 1.5
HGB 13.0 0.7 1.5
MCV 91.0 0.4 1.0
PLT 242 3.2 4.0
In the above example, the RBC measured value is 4.43 and the acquired CV is
0.8%. 0.8% of 4.43 is 0.035, 4.43 ± 0.035 is 4.465 and 4.395. Therefore, the CV
of 0.8% indicates that in 6 of 10 measurements, the acquired value is between
4.395 and 4.465.
Reference
The CV of a Sample of Low Measured Values (Low Concentration)
On the hematology analyzer, the blood cells passing through the apertures are
counted. The reproducibility depends on the number of counted cells. When this
number increases, the reproducibility is improved. Therefore, if the number of
counted cells is small (a sample with low concentration is measured), the variation
increases. For example, the CV is within 4% when counting 300,000 PLT, but the
CV increases to over 4% when counting 100,000 PLT. This is a common feature
for any hematology analyzer.

2. TROUBLESHOOTING
Checking Procedure 1. Select “FACTORY” as the operator on the OPERATORS & PASSWORDS
screen.
i) Press MENU key → SETTINGS key → OPERATORS & PASSWORDS

key
→ SELECT OPERATOR key → Enter password

ii) Press FACTORY key→
“4321” → ENTER key
2. Measure 10 samples.
When measuring MEK-5DN hematology control:

i) Press MENU key → OTHER OPERATIONS key → SERVICE
MAINTENANCE key → ADVANCED SETTINGS key →
MEASUREMENT MODE key
ii) Select ON for “CONTINUE 5D MODE”.
Set 10 samples in the rack and measure them.

Press MENU key → OTHER OPERATIONS key → SERVICE
MAINTENANCE key → CONTINUOUS MEASUREMENT key → CLOSED
10 SAMPLES key
3. Display the result and check the CV data.

MAINTENANCE key → 10 data X-CV key
4. When MEK-5DN hematology control is measured, return the “CONTINUE 5D

MODE” setting to OFF, then change the operator to the previous operator.
Poor Reproducibility If the reproducibility check result is poor, find the cause and countermeasure from
Problems the following tables.
Poor WBC Reproducibility

The following may be the main reasons for poor WBC reproducibility.
• Measuring units and fluid paths are dirty
• Deterioration of reagents
• Samples are not stirred enough
• Hematology analyzer malfunction

2. TROUBLESHOOTING
Measurement bath, rinse unit or fluid path is dirty

Possible Cause/Criteria Countermeasure
Press the Clean key on the front panel to perform cleaning.
WBC measurement bath is dirty. Clean the WBC measurement bath. Refer to the operator’s manual.
Replace the WBC measurement bath with a new one.
Replace the hemoglobin filter below air trap with a new one.
Drain path of WBC measurement bath is dirty.
Clean the air trap.
Clean the rinse units of the closed mode and manual mode. Refer to
Rinse unit is dirty.
the operator’s manual.
Deterioration of reagents
Replace the reagents with the new ones and handle them as indicated on their
manual. Avoid direct sunlight and use the reagents in appropriate condition.
Samples are not stirred enough

In manual mode measurement, the sample was not stirred Stir the sample thoroughly before measurement. Follow the
thoroughly before measurement. instructions in the operator’s manual.
In closed mode measurement, the sample tubes which are
not specified in the operator’s manual were used and Only use the specified sample tubes.
therefore, the samples could not be stirred thoroughly.
In closed mode measurement, the samples were not
Stir the samples thoroughly before setting them in the rack.
stirred thoroughly before setting them into the rack.
In closed mode measurement, the sample tube had a bar
code label which cannot be read, therefore, the sample Attach the bar code label so that it does not come off.
could not be stirred properly.
In closed mode, the mixing unit is dirty. Clean the mixing unit. Refer to the operator’s manual.
In closed mode, the sample rack is dirty. Clean the sample rack. Refer to the operator’s manual.
Hematology analyzer malfunction

The appropriate amount of hemolysing reagent is not Replace the Hemolynac3 tube assy with a new one.
dispensed. Replace the MP-820V Complex Pump Unit with a new one.
Circuit malfunction Replace the MC-820V CBC Measuring Unit with a new one.
(Perform circuit check by pressing MENU key → In closed mode, replace the MS-820V Closed Sampler Unit
OTHER OPERATIONS key → USER MAINTENANCE with a new one.
key → CIRCUIT CHECK key and check that the WBC In manual mode, replace the MS-821V Open Sampler Unit
is outside 8.0 ±5%) with a new one.

2. TROUBLESHOOTING
Poor WBC 5 Part Differential Reproducibility

The diluent is dirty. Replace the diluent with a new one.
The diluent bottle is dirty. Clean the diluent bottle.
Press the Clean key on the front panel to perform
The fluid path is dirty.
cleaning.
Noise through AC source. Connect the power cord to another AC outlet.
Perform equipotential grounding.
High Noise interference. Move the instrument which is producing the noise away
background from the hematology analyzer.
noise The reagents are used outside the specified
Use the reagents in the appropriate temperature.
temperature range.
The specified reagents are not used. Only use the specified reagents.
The Hemolynac3 hemolysing reagent is Clean the tube and prime the fluid path (PRIME ON
connected to the HEMO5 inlet. INSTALLATION on the OTHER OPERATIONS screen).
No scattergrams.
Scattergram Scattergrams are too widely scattered to
group them. Refer to the “Checking Scattergrams” section.
problems
The scattergrams are grouped but they are
not in the allotted area.
The MEK-3DN hematology control is
Use MEK-5DN hematology control.
measured.
The blood sample collected from a person is
Use the blood sample within 12 hours after collection.
left for more than 12 hours.
When using the human blood for the reproducibility
Specific blood check, use the blood sample within the following values.
characteristics WBC: 4.0 to 9.0 × 103/µL
The blood sample with low WBC is NE%: 40 to 70%
measured. LY%: 20 to 45%
MO%: 2 to 10%
EO%: 2 to 10%
BA%: 0 to 3%
The pinch valve tube is clogged. Replace the pinch valve tube with a new one.
The sample is Replace the unit with a new one. Refer to Section 4.
failure.
diluted too
much. UT-7146 MASTER board failure. Replace the board with a new one. Refer to Section 4.
The pump tube of the MP-520V Pump Unit Replace the pump tube with a new one. Refer to Section
is damaged. 4.
MP-520V Pump Unit failure. Replace the unit with a new one. Refer to Section 4.
Leak in the tube connection. Check the tubes.
Too many The environment temperature is outside 15
The environment temperature must be within 15 to 30°C.
ghosts. to 30°C.

2. TROUBLESHOOTING
Poor HGB Reproducibility

The following may be the main reasons for poor HGB reproducibility.
• Deterioration of reagents

WBC measurement bath is dirty. Clean the WBC measurement bath. Refer to the operator’s manual.
Replace the WBC measurement bath with a new one.
Replace the hemoglobin filter below air trap with a new one.
Drain path of WBC measurement bath is dirty.
Clean the air trap.
Clean the rinse units of the closed mode and manual mode. Refer to
the operator’s manual.
Deterioration of reagents
Replace the reagents with the new ones and handle them as indicated on their
manual. Avoid direct sunlight and use the reagents in appropriate condition.


The appropriate amount of hemolysing reagent is not Replace the Hemolynac3 tube assy with a new one.
dispensed. Replace the MP-820V Complex Pump Unit with a new one.
Replace the MP-820V Complex Pump Unit with a new one.
In closed mode, replace the MS-820V Closed Sampler Unit
HGB sample error. with a new one.
In manual mode, replace the MS-821V Open Sampler Unit
with a new one.
HGB sensor output failure Adjust VR of the HGB unit so that HGB ON item is 3.0 V ±
(Perform sensor check by pressing MENU key → OTHER 1 V.
OPERATIONS key → SERVICE MAINTENANCE key Replace the MC-820V CBC Measuring Unit with a new
→ MONITOR key → SENSOR MONITOR key and one.
check that the HGB ON item is outside 1.5 to 4.5 V range) Replace the UT-7147 SLAVE CBC Board with a new one.

2. TROUBLESHOOTING
Poor RBC Reproducibility

The following may be the main reasons for poor RBC reproducibility.

Clean the RBC measurement bath and sub bath. Refer to the
RBC measurement bath and sub bath are dirty.
operator’s manual.
Replace the RBC measurement bath and sub bath with the new ones.
Filter below the RBC measurement bath is dirty. Replace the filter with a new one.
Clean the rinse unit of closed mode and manual mode. Refer to the


Circuit malfunction
(Perform circuit check by pressing MENU key → Replace the MC-820V CBC Measuring Unit with a new one.
OTHER OPERATIONS key → USER MAINTENANCE
key → CIRCUIT CHECK key and check that the RBC is Replace the UT-7147 SLAVE CBC Board with a new one.
outside 1.60 ±5%)
In closed mode, replace the MS-820V Closed Sampler Unit
RBC sample error. with a new one.
In manual mode, replace the MS-821V Open Sampler Unit
with a new one.

2. TROUBLESHOOTING
Poor PLT Reproducibility

The following may be the main reasons for poor PLT reproducibility.
• Background noise is high
PLT measurement is a highly sensitive measurement. Therefore, the background
noise must be within the acceptable range (PLT < 10 × 103/µL). Before checking
the PLT reproducibility, check the background noise.
• Inappropriate measuring settings
• Dilution error
Background noise is high

The diluent is dirty. Replace the diluent with a new one.
The diluent bottle is dirty. Clean the diluent bottle.
Clean the measurement bath and sub bath. Refer to the operator’s manual.
The fluid path is dirty. Clean the aperture. Refer to the operator’s manual.
Replace the measurement bath and sub bath with new ones.
Replace the aperture cap with a new one.
Clean the rinse unit of manual mode and closed mode. Refer to the
Replace the sampling nozzle with a new one. Refer to the operator’s
The sampling nozzle is clogged.
manual.
Noise through AC source. Connect the power cord to other AC outlet.
Perform equipotential grounding.
Noise interference. Move the instrument which is producing the noise away from the
hematology analyzer.
The aperture is damaged. Replace the aperture cap with a new one.
The MC-820V CBC Measuring Unit failure. Replace the unit with a new one. Refer to Section 4.
Replace the UT-7147 SLAVE CBC Board with a new one. Refer to Section
4.
The circuit failure.
Replace the MC-820V CBC Measuring Unit with a new one. Refer to
Section 4.
Circuit malfunction
(Perform circuit check by pressing MENU
key → OTHER OPERATIONS key →
Replace the UT-7160 POWER Board with a new one.
USER MAINTENANCE key → CIRCUIT
CHECK key and check that the PLT is
outside 160 ±5%)

2. TROUBLESHOOTING

Inappropriate measuring settings

Check that the sensitivity and threshold settings are set as below.
RBC SENSITIVITY: 5
RBC THRESHOLD: AUTO
PLT THRESHOLD: 5
Dilution error
MS-820V Closed Sampler Unit failure. Replace the unit with a new one.
MS-821V Open Sample Unit failure. Replace the unit with a new one.

The aperture is dirty. Clean the aperture. Refer to the operator’s manual.
Replace the aperture cap with a new one. Refer to the
The aperture is damaged.
Circuit malfunction Replace the UT-7147 SLAVE CBC Board with a new one.
(Perform circuit check by pressing MENU key → Replace the MC-820V CBC Measuring Unit with a new one.
OTHER OPERATIONS key → USER MAINTENANCE
key → CIRCUIT CHECK key and check that the PLT is Replace the UT-7160 POWER Board with a new one.
outside 160 ±5%)

2. TROUBLESHOOTING
Poor HCT or MCV Reproducibility

The following may be the main reasons for poor HCT or MCV reproducibility.
• Fluid path is dirty
• Inappropriate measuring settings
Fluid path is dirty

The aperture may be dirty. Clean it by referring to the operator’s manual.
Inappropriate measuring settings

Check that the sensitivity and threshold settings are set as below.
RBC SENSITIVITY: 5
RBC THRESHOLD: AUTO

The aperture is damaged. Replace the aperture cap with a new one. Refer to the operator’s manual.
Circuit malfunction
(Perform circuit check by pressing MENU Replace the UT-7147 SLAVE CBC board with a new one.
key → OTHER OPERATIONS key →
USER MAINTENANCE key → CIRCUIT
CHECK key and check that the RBC is Replace the MC-820V CBC Measuring Unit with a new one.
outside 1.60 ±5%)
RBC sample error. In closed mode, replace the MS-820V Closed Sampler Unit with a new one.
In manual mode, replace the MS-821V Open Sampler Unit with a new one.

2. TROUBLESHOOTING
Checking Accuracy
Before checking accuracy, measure a MEK-5D hematology control with the same
procedure described in the “Checking the Reproducibility” section. Confirm that
the obtained sample data is within the acceptable range on the assay sheet attached
to the hematology control. If the data is outside this range or on the borderline,
calibrate the hematology analyzer with the following procedure.
Changing the Operator to Select “FACTORY” as the operator on the OPERATORS & PASSWORDS screen.
“FACTORY”
1. Press MENU key → SETTINGS key → OPERATORS & PASSWORDS key.
→ SELECT OPERATOR key → Enter password “4321”

2. Press FACTORY key→
→ ENTER key.
Calibration Procedure The CBC parameters (WBC, RBC, HGB, MCV, PLT, RDW and MPV) are
calibrated using the MEK-3DN hematology control as a calibrator. This calibrator
can be used when it is within 3 days after opening, stored at 2 to 8°C and handled
appropriately according to its manual.
1. Set the sample tube containing the MEK-3DN calibrator in rack position no. 1.
2. Press the MENU key → OTHER OPERATIONS key → SERVICE

10 SAMPLES key to measure the calibrator 10 times.

MAINTENANCE key → 10 DATA X-CV key to display the result and check
the CV data.

2. TROUBLESHOOTING
4. Calibrate the new calibration coefficient for each parameter from the mean
value according to the equation below.
Calibrator assay value

New calibration coefficient = Previous calibration coefficient ×
Mean measured value
5. Display the CAL CLOSED screen.

Press the MENU key → CALIBRATION key → CLOSED key.
6. On the CALIBRATION screen, press the WBC key to calibrate WBC, press the
RBC to calibrate RBC parameters and press the PLT to calibrate the PLT
parameters.
7. Use the arrow keys to move the cursor to the calibration coefficient box of the
parameter you want to calibrate.
8. Use the numerical keys on the screen to enter the new calibration coefficient
you calculated in step 4. Press the Enter key to register the value.
9. Repeat steps 6 to 8 to change the calibration coefficient for other parameters.
10. Set the sample tube containing the MEK-3DN calibrator in rack position no. 1
and measure it 10 times.
Press the MENU key → OTHER OPERATIONS key → SERVICE

10 SAMPLES key.

MAINTENANCE key → 10 DATA X-CV key to display the result and check
that the average values (X values) are within the range on the assay sheet
provided with the calibrator.
If the parameters are not within the range on the assay sheet, calibrate the CBC
parameters again.
2. TROUBLESHOOTING
Checking Scattergram
Checking Scattergram
Procedure WARNING
Always wear rubber gloves to protect yourself from infection when
handling and measuring blood samples.
1. Prepare 10 blood samples of a healthy person. The blood sample must be

within 8 hours after collection.
2. Set them in the sample rack and measure them in closed mode.
3. Check that none of the following flags appears.
• Blasts
• Immature Granulocyte
• Left Shift
• Atypical Lymphocytes
Flags appear in this area
4. Display the DIFF GAIN (FINE) screen.
Press the MENU key → SETTINGS key → SENSITIVITY THRESHOLD key

→ DIFF GAIN key → ADJUST GAIN (FINE) key.

2. TROUBLESHOOTING
5. Enter the sequence number of the data measured in step 2 in the NEW
SAMPLE SEQ# box and press the LOAD NEW SCATTERGRAM key to
display the scattergram of the selected data.
6. Check the following points.
• The scattergrams are oval in shape and are not touching the division line.
• The scattergrams are displayed in the MO, LY and NE area on the left
scattergram.
• There are not many scattergrams displayed in the upper area on the center
and right scattergrams.
• In the MO PEAK table and on the ACTUAL PEAK LIST screen, the
AVERAGE ACTUAL PEAK and IDEAL PEAK values are the same. To
display the ACTUAL PEAK LIST screen, press the LIST key on the DIFF
GAIN (FINE) screen.
Each MO PEAK target value and optimum range in parentheses are shown
below.
MO FS PEAK: 189 (186 to 192)
MO FL PEAK: 49 (47 to 51)
MO SD PEAK: 23 (21 to 23)
NE
MO PEAK table
MO
LY

2. TROUBLESHOOTING
Scattergram Problems Differential Parameters Rough Gain Problems

Refer to the “Poor Optical Reproducibility Problems” section.
Differential Parameters Fine Gain Problems

The scattergrams are grouped but they are not in the area allotted by the
division lines
The gain of the amplifier which amplifies the scattered light pulses may be set
incorrectly. Adjust the gain. Refer to the “Measuring Polymer Microsphere
The scattergrams are not grouped

The reagents which are not specified are used. Only use the specified reagents.
Specific characteristics of the sample.
e.g. WBC too high There is no problem on the hematology analyzer.
More than 12 hours elapsed after sample collection Measure the sample within 12 hours after collection.
MEK-3D is measured
Adjust gain. Refer to the “Measuring Polymer Microsphere
CV of scattergram is out of range.
The gain of the amplifier which amplifies the scattered Adjust gain. Refer to the “Measuring Polymer Microsphere
light pulses is set incorrectly. Suspensions for Checking CV” section.
Sample failure. Leakage or clog in the fluid circuit. Contact your Nihon
Kohden distributor.
No scattergrams

turned off.
The MO-820V Laser Optical Unit
Connect the unit.
connector is disconnected.
Replace the pinch valve tubes with new ones. Refer
The pinch valve tubes are clogged.
to Section 4.
No sample flow into
the flow cell.
failure.
inappropriate.

2. TROUBLESHOOTING
Blasts or Immature Gr appears

Specific characteristics of the
There is no problem on the hematology analyzer.
sample. More than 12 hours elapsed There is no problem on the hematology analyzer.
after sample collection Measure the sample within 12 hours after collection.
FS gain is high and many scatters
Adjust gain. Refer to the “Checking Scattergram
appear in the Blasts and Immature Fine gain must be adjusted.
Procedure” section.
Gr area.
Adjust gain. Refer to the “Measuring Polymer
CV of scattergram is out of range. Gain must be adjusted.
Microsphere Suspensions for Checking CV” section.
Left Shift appears

FL gain is not appropriate and Adjust gain. Refer to the “Checking Scattergram
Fine gain must be adjusted.
MO and NE scattergrams overlap. Procedure” section.
Atypical Ly appears
FS gain is low and LY and MO Adjust gain. Refer to the “Checking Scattergram
scattergrams overlap. Procedure” section.
FS gain is high and LY and ghosts Adjust gain. Refer to the “Checking Scattergram
overlap. Procedure” section.
Micosphere Suspensions for Checking CV” section.
The environment temperature The environment temperature must be within 15 to
Too many ghosts.
is out of range. 30°C.

2. TROUBLESHOOTING
Solving Problems from Error Messages
Error Messages A001: No diluent (type 1)

This message appears when the sensor in the tank does not detect the diluent during
measurement, priming or cleaning.

The tube between the hematology analyzer
Check the tube connection and if necessary, replace
and diluent is squeezed, bent, clogged or
the tube with a new one.
there is leakage.
Leakage in the fluid path. Contact your Nihon Kohden distributor.
No diluent in the Pump tubes are damaged. Replace the tubes with the new ones.
tank. MP-520V Pump Unit failure. Replace the unit with a new one.
UT-7148 SLAVE MS Board failure. Replace the board with a new one.
The electromagnetic valve failure. Contact your Nihon Kohden distributor.
The sensor is damaged. Replace the sensor with a new one.
Failure of the sensor When the hematology analyzer is left Press the Reset and Power keys together to turn the
in the tank unused, the diluent inside the tank may power off, then press the Power key to on.
evaporate, adheres to the sensor and the
Perform priming.
sensor opration becomes unstable.
A002: No diluent (type 2)

This message appears when there are air bubbles in the fluid path during
measurement.
When the hematology analyzer is left unused, Press the Clean key to perform cleaning, press
the diluent inside the tank may evaporate and the Reset and Power keys together to turn the
Air bubbles enter into the fluid path as air bubbles. power off, then press the Power key to on.
Refill the diluent, press the Reset and Power keys
Run out of diluent during the sensor
Failure of the sensor together to turn the power off, press the Power
malfunction (e.g. detecting diluent when there
in the tank key to on and then press the Clean key to perform
is none).
cleaning.
Air bubbles on the liquid sensor. Perform priming.
Failure of the sensor
in the 4-channel Voltage adjustment error of the liquid sensor. Adjust the voltage.
liquid sensor unit Replace the 4-channel liquid sensor unit with a
The liquid sensor failure.
new one.

2. TROUBLESHOOTING
A003: No diluent (flowcyte type 1)

This message appears when the sensor in the sheath tank does not detect the diluent
during measurement, priming or cleaning or when the sensor in the 4-channel
liquid sonsor unit does not detect the diluent.

The tube between the hematology analyzer and Check the tube connection and if
diluent is squeezed, bent, clogged or there is leakage. necessary, replace the tube with a new one.
MP-822V Sheath Pump Unit failure. Replace the unit with a new one.
No diluent in the Pump tubes are damaged. Replace the tubes with the new ones.
sheath tank.
MP-520V Pump Unit failure. Replace the unit with a new one.
MC-821V Flow Cytometry Piping Unit failure. Replace the unit with a new one.
Electromagnetic valve failure. Contact your Nihon Kohden distributor.
Failure of the sensor Air bubbles on the sensor which cause error. Press the Clean key to perform cleaning.
in the sheath tank MP-822V Sheath Pump Unit failure. Replace the unit with a new one.
Air bubbles on the liquid sensor. Perform priming.
Failure of the sensor
in the 4-channel Voltage adjustment error of the liquid sensor. Adjust the voltage.
liquid sensor unit Replace the 4-channel liquid sensors unit
Liquid sensor failure.
with a new one.
A004: No diluent (flowcyte type 2)

This message appears when the sensor in the 4-channel liquid sensor unit detects
the diluent but the sensors in the sheath tank does not detect the diluent during
measurement, priming or cleaning.

The tube between the hematology analyzer and
Check the tube connection and if necessary,
diluent is squeezed, bent, clogged or there is
replace the tube with a new one.
leakage.
No diluent in the MP-821V Sample Pump Unit failure. Replace the unit with a new one.
sheath tank. Pump tubes are damaged. Replace the tubes with the new ones.
Failure of the sensor Air bubbles on the sensor which cause error. Press the Clean key to perform cleaning.
in the sheath tank MP-822V Sheath Pump Unit failure. Replace the unit with a new one.
Failure of the sensor Air bubbles on the liquid sensors. Perform priming.
in the 4-channel Replace the 4-channel sensor unit with a new
liquid sensor unit Liquid sensor failure.
one.

2. TROUBLESHOOTING
A005: No detergent (CLEANAC)

This message appears when the sensor in the 4-channel liquid sensor unit does not
detect the detergent during priming or cleaning.
CLEANAC detergent is squeezed, bent, clogged or
there is leakage.
MP-821V Sample Pump Unit failure. Replace the unit with a new one.
No detergent
flow Pump tubes are damaged. Replace the tubes with the new ones.
Failure of the Air bubbles on the liquid sensors. Perform priming.
sensor in the 4- Voltage adjustment error of the liquid sensors. Adjust the voltage.
channel liquid Replace the 4-channel liquid sensor unit with a
sensor unit Liquid sensor failure.
new one.
A006: No detergent (CLEANAC•3)

detect the detergent during measurement, priming or cleaning.

CLEANAC•3 detergent is squeezed, bent, clogged or
there is leakage.
MC-821V Flow Cytometry Piping Unit failure. Replace the unit with a new one.
No detergent
flow Pump tubes are damaged. Replace the tubes with the new ones.
new one.
A007: No hemolysing reagent (HEMOLYNAC•3)

detect the hemolysing reagent during measurement, priming or cleaning.

HEMOLYNAC•3 hemolysing reagent is squeezed,
bent, clogged or there is leakage.
No hemolysing MP-820V Complex Pump Unit failure. Replace the unit with a new one.
reagent flow UT-7146 MASTER Board failure. Replace the board with a new one.
UT-7155 DRIVER4 Board failure. Replace the board with a new one.
new one.

2. TROUBLESHOOTING
A008: No hemolysing reagent (HEMOLYNAC•5)

detect the hemolysing reagent during measurement, priming or cleaning.

HEMOLYNAC•5 hemolysing reagent is squeezed,
bent or clogged or there is leakage.
No hemolysing MP-820V Complex Pump Unit failure. Replace the unit with a new one.
reagent flow UT-7146 MASTER Board failure. Replace the board with a new one.
channel liquid Replace the 4-channel sensor unit with a new
one.
A009: WBC priming error

This message appears when diluent cannot be primed during priming the WBC
manometer (manometer upper or lower sensor does not detect the diluent).

Liquid cannot be WBC manometer upper sensor adjustment error Adjust the sensor.
detected even when
WBC manometer lower sensor adjustment error Adjust the sensor.
there are enough liquid
(LED does not light) MC-820V CBC Measuring Unit failure. Replace the unit with a new one.
Air bubbles in the WBC aperture cap is not attached properly. Reattach the WBC aperture cap.
manometer MC-820V CBC Measuring Unit failure. Replace the unit with a new one.
A010: RBC priming error

This message appears when diluent cannot be primed during priming the RBC
manometer (manometer upper or lower sensor does not detect the diluent).

Liquid cannot be RBC manometer upper sensor adjustment error Adjust the sensor.
detected even when
RBC manometer lower sensor adjustment error Adjust the sensor.
Air bubbles in the RBC aperture cap is not attached properly. Reattach the RBC aperture cap.

2. TROUBLESHOOTING
A021: WBC fluid level 1

This message appears when manometer upper sensor does not detect no liquid at
the start of WBC measurement or draining liquid from the manometer.
Liquid is drained but WBC manometer upper sensor adjustment error Adjust the sensor.
liquid cannot be detected WBC manometer is dirty Clean the WBC manometer.
WBC pump tube is damaged. Replace the pump tube with a new one.
Replace the unit or UT-7153 DRIVER2
MP-520V Pump Unit (WBC) failure.
Board with a new one.
Liquid cannot be drained MC-820V CBC Measuring Unit failure. Replace the unit with a new one.
Replace the UT-7153 DRIVER2 Board or
Electromagnetic valve failure. UT-7147 SLAVE CBC Board with a new
one.

This message appears when manometer upper sensor detects no liquid but the
lower sensor does not detect no liquid at the start of WBC measurement or draining
liquid from the manometer.

Liquid is drained but WBC manometer lower sensor adjustment error Adjust the sensor.
liquid cannot be detected WBC manometer is dirty Clean the WBC manometer.
WBC pump tube is damaged. Replace the pump tube with a new one.
MP-520V Pump Unit (WBC) failure.
one.

This message appears when manometer lower sensor does not detect the liquid
during WBC measurement or aspirating sample.

Cannot produce measuring WBC pump tube is damaged. Replace the pump tube with a new one.
pressure MC-820V CBC Measuring Unit failure. Replace the unit with a new one.
Reattach the WBC aperture cap
Measuring pressure can be WBC aperture cap is not attached properly.
properly.
produced but there is leakage
Clean the aperture or replace the
Measuring pressure can be WBC aperture is clogged.
aperture cap with a new one.
produced but there is clog

2. TROUBLESHOOTING
A024: WBC bubble 1

This message appears when manometer upper or lower sensor does not detect the
liquid during WBC measurement or at the start of draining manometer.

Liquid cannot be WBC manometer upper sensor adjustment error Adjust the sensor.
detected even when
A025: WBC bubble 2

This message appears when manometer upper sensor detects the liquid during
WBC measurement or draining manometer (from upper sensor OFF to lower
sensor OFF).

Air bubbles in the manometer MC-820V CBC Measuring Unit failure. Replace the unit with a new one.
A026: WBC bubble 3

This message appears when manometer lower sensor detects no diluent during
WBC measurement or aspirating sample (from lower sensor ON to upper sensor
ON).

A027: WBC bubble 4

This message appears when WBC measurement or aspirating sample time is too
short (from lower sensor ON to upper sensor ON).

Replace the WBC aperture cap with a new
The aperture is too large. WBC aperture is damaged.
one.
A028: WBC manomtr dirty

This message appears when the voltage for detecting liquid in the manometer is
near threshold.

WBC manometer is dirty. Clean the WBC manometer.
Contamination on the
WBC manometer upper sensor adjustment error Adjust the sensor.
WBC manometer

2. TROUBLESHOOTING
A029: WBC clogged

This message appears when manometer lower sensor detect the liquid but the upper
sensor does not detect the liquid during WBC measurement or aspirating sample.

WBC aperture is clogged. Clean or replace the WBC aperture cap.
Measuring pressure can be RBC aperture cap is attached instead of Attach the WBC aperture cap to the WBC
produced but there is clog WBC. side.
Cannot produce measuring WBC pump cap is damaged. Replace the pump tube with a new one.
Measuring pressure can be WBC aperture cap is not attached properly. Reattach the WBC aperture cap properly.
produced but there is leakage MC-820V CBC Measuring Unit failure. Replace the unit with a new one.
A030: WBC sample error

This message appears when the voltage between the electrodes is out of acceptable
range.

Something wrong of the The environment temperature must be from
The diluent temperature is too low.
diluted sample for WBC 15 to 30°C.
count Specified reagents are not used. Only use the specified reagents.
A031: WBC hardware noise

This message appears when there is baseline wondering during measurement.
A032: WBC software noise
This message appears when the counted pulse are not stable in time sequence.

2 pin power cord is used. Use 3 pin power cord.
Power line Ground lead is not connected. Perform equipotentail grounding.

Do not share the power source with any
Decrease in the power voltage.
other instrument.
WBC aperture is dirty. Clean the WBC aperture cap.
Replace the WBC aperture cap with a new
WBC aperture is damaged.
one.
The screw attaching the measurement bath is
Measuring unit failure. Tighten the screw.
loose.
MC front shield cover and MC front chassis are
Remove the cause.
not insulated (there may be leakage).
Sample aspiration is not stable (clog alarm). Refer to “A029: WBC clogged” table.
Influence from other Refer to “! appears on the right of WBC
malfunction. Poor hemolysation of the sample. measured valve “ in “Solving Problems
from Flags” section.

2. TROUBLESHOOTING
A041: RBC fluid level 1

This message appears when manometer upper sensor does not detect no liquid at
the start of RBC measurement or draining liquid from the manometer.
Liquid is drained but RBC manometer upper sensor adjustment error Adjust the sensor.
liquid cannot be detected RBC manometer is dirty Clean the RBC manometer.
(LED does not go out) MC-820V CBC Measuring Unit failure. Replace the unit with a new one.
RBC pump tube is damaged. Replace the pump tube with a new one.
MP-520V Pump Unit (RBC) failure.
one.

This message appears when manometer upper sensor detects no liquid but the
lower sensor does not detect no liquid at the start of RBC measurement or draining
liquid from the manometer.

Liquid is drained but RBC manometer lower sensor adjustment error Adjust the sensor.
liquid cannot be detected RBC manometer is dirty Clean the RBC manometer.
(LED does not go out) MC-820V CBC Measuring Unit failure. Replace the unit with a new one.
RBC pump tube is damaged. Replace the pump tube with a new one.
MP-520V Pump Unit (RBC) failure.
one.

This message appears when manometer lower sensor does not detect the liquid
during RBC measurement or aspirating sample.

Cannot produce measuring RBC pump tube is damaged. Replace the pump tube with a new one.
Measuring pressure can be RBC aperture cap is not attached properly. Reattach the RBC aperture cap properly.
Measuring pressure can be RBC aperture is clogged. Clean or replace the RBC aperture cap.
produced but there is clog MC-820V CBC Measuring Unit failure. Replace the unit with a new one.

2. TROUBLESHOOTING
A044: RBC bubble 1

This message appears when manometer upper or lower sensor detects no liquid
during RBC measurement or at the start of draining manometer.

Liquid cannot be RBC manometer upper sensor adjustment error Adjust the sensor.
detected even when
A045: RBC bubble 2

This message appears when manometer upper sensor detects the liquid during RBC
measurement or when draining manometer (from upper sensor OFF to lower sensor
OFF).

A046: RBC bubble 3

This message appears when manometer lower sensor detects no liquid during RBC
measurement or aspirating sample (from lower sensor ON to upper sensor ON).
A047: RBC bubble 4

This message appears when RBC measurement or aspirating sample time is too
short (from lower sensor ON to upper sensor ON).
Replace the RBC aperture cap with a new
RBC aperture is damaged.
one.
The aperture is too large.
WBC aperture cap is attached instead of Attach the RBC aperture cap to the RBC
RBC. side.
A048: RBC manomtr dirty

This message appears when the voltage for detecting liquid in the manometer is
near threshold.
RBC manometer is dirty. Clean the RBC manometer.
Contamination on the
RBC manometer upper sensor adjustment error Adjust the sensor.
RBC manometer

2. TROUBLESHOOTING
A049: RBC clogged

This message appears when manometer lower sensor detects the liquid but the
upper sensor does not detect the liquid during RBC measurement or aspirating
sample.

Clean or replace the RBC aperture cap
Measuring pressure can be RBC aperture is clogged.
with a new one.
produced but there is clog
Cannot produce measuring RBC pump tube is damaged. Replace the pump tube with a new one.
Measuring pressure can be RBC aperture cap is not attached properly. Reattach the RBC aperture cap properly.
A050: RBC sample error

This message appears when the voltage between the electrodes is out of acceptable
range.

Something wrong of the The environment temperature must be from
The diluent temperature is too low.
diluted sample for RBC 15 to 30°C.
count Specified reagents are not used. Only use the specified reagents.
A051: RBC hardware noise

This message appears when there is baseline wondering during measurement.
A052: RBC software noise
This message appears when the counted pulses are not stable in time sequence.

2 pin power cord is used. Use 3 pin power cord.
Power line Ground lead is not connected. Perform equipotentail grounding.

Do not share the power source with any
Decrease in the power voltage.
other instrument.
RBC aperture is dirty. Clean the RBC aperture.
Replace the RBC aperture cap with a new
RBC aperture is damaged.
one.
The screw attaching the measurement bath is
Measuring unit failure. Tighten the screw.
loose.
MC front shield cover and MC front chassis are
Remove the cause.
not insulated (there may be leakage).
Influence from other Sample aspiration is not stable (clog alarm). Refer to “A049: RBC clogged” table.
malfunction. Background noise increases. Refer to “Background Noise Problems”.

2. TROUBLESHOOTING
A061: HGB voltage low

This message appears when the HGB BLANK (diluent) voltage is less than 1.5 V.
WBC measurement bath is dirty. Clean or replace the WBC measurement bath.
Secular distortion
HGB voltage adjustment error. Adjust the HGB voltage.
Circuit error MC-820V CBC Measuring Unit failure. Replace the unit with a new one.
HGB measurement started when there was
Erroneous operation blood sample in the WBC measurement bath Perform priming and measure again.
or the hematology analyzer was drained.
A062: HGB voltage high

This message appears when the HGB BLANK (diluent) voltage is more than 4.5 V.
Secular distortion HGB voltage adjustment error. Adjust the HGB voltage.
HGB voltage was adjusted when there was
Erroneous operation blood sample in the WBC measurement bath Perform priming and adjust the HGB voltage.
or the hematology analyzer was drained.
A063: HGB circuit error

This message appears when the voltage is more than 0.5 V at HGB LED off.
Measurement bath cover is open. Close the cover.
Light interference
Front cover is open. Close the cover.
A071: Mixing error

This message appears when sample stirring is insufficient.
The sample tube cannot Remove the label or attach it properly and
The label on the sample tube is coming off.
be stirred smoothly due measure again.
to resistance. The sample rack is dirty. Clean the sample rack.
The timing belt of the sampler unit is
Replace the timing belt with a new one.
Stirring unit failure. damaged.

2. TROUBLESHOOTING
A081: Laser switch off

This message appears when there is no laser during measurement.

turned off.
MO-820V Laser Optical Unit connector is
Connect the unit.
disconnected.
A082: Optical count error

This message appears when the number of counts differs greatly between optical
WBC count and impedance WBC count.

Specific blood The MEK-3DN hematology control is
No problem on the hematology analyzer.
characteristics measured.
CV of scattergram is Gain must be adjusted using polymer Adjust gain. Refer to the “Measuring Polymer
out of range. microsphere suspensions. Microsphere Suspensions for Checking CV” section.
The gain of the

amplifier which
Gain must be adjusted using polymer Adjust gain. Refer to the “Measuring Polymer
amplifies the
microsphere suspensions. Microsphere Suspensions for Checking CV” section.
scattered light pulses
is set incorrectly.
The pinch valve tube is clogged. Replace the pinch valve tube with a new one.
The sample flow is MP-520V Pump Unit failure. Replace the unit with a new one. Refer to Section 4.
not stable. MP-821V Sample Pump Unit failure. Replace the unit with a new one. Refer to Section 4.
No waveform is Diff gain (FS THR) setting is not
Set FS THR to 10.
detected. appropriate.
MP-820V Complex Pump Unit failure. Replace the unit with a new one. Refer to Section 4.
Sample failure
Leakage or clog in the fluid path. Contact your Nihon Kohden distributor.
The environment temperature is outside The environment temperature must be within 15 to
Too many ghosts.
15 to 30°C. 30°C.

2. TROUBLESHOOTING
System Error Messages E032: Circuit error

This message appears when there is an error in self check of CBC circuit at power
on.

E033: Check settings

This message appears when there is an error in backup data check of calibration
coefficient at power on.
The battery on the UT-7146 MASTER Replace the battery with a new one.
Board is weak. Replace the board with a new one.
E101: CLOSED SAMPLER ERROR

E102: OPEN SAMPLER ERROR

MS-821V Open Sampler Unit failure. Replace the unit with a new one.
E103: TABLE ERROR

MS-822V Table Unit failure. Replace the unit with a new one.
E104: MIXING ERROR


2. TROUBLESHOOTING
E105: BARCODE ERROR

Replace the bar code reader with a new
ZK-821V Bar Code Reader failure.
one.
E106: DILUTER ERROR

E107: LYSE PUMP ERROR

E108: SAMPLE PUMP ERROR

MP-821V Sample Pump Unit failure. Replace the unit with a new one.
Replace the unit with a new one.
failure.
E109: SHEATH PUMP ERROR

E110: 5DIFF ROTARY PUMP ERROR

MP-520VJ (5DIFF) Pump Unit failure. Replace the unit with a new one.
Replace the unit with a new one.
failure.

2. TROUBLESHOOTING
E111: WBC ROTARY PUMP ERROR

MP-520V (WBC) Pump Unit failure. Replace the unit with a new one.
E112: RBC ROTARY PUMP ERROR

MP-520V (RBC) Pump Unit failure. Replace the unit with a new one.
E113: WBC SUB BATH ERROR

MB-820V Sub Bath Unit failure. Replace the unit with a new one.
E114: RBC SUB BATH ERROR

MB-820V Sub Bath Unit failure. Replace the unit with a new one.
E115: WARMER ERROR

ZY-820V Warmer Unit failure. Replace the unit with a new one.
E201: MASTER BD ERROR

E202: SLAVE MS BD ERROR


2. TROUBLESHOOTING
E203: SLAVE CBC BD ERROR

E301: MASTER-MS COMM ERROR

Cable is disconnected. Connect the cable.
E302: MASTER-CBC1 COMM ERROR

E303: MASTER-CBC2 COMM ERROR

E304: MS-CBC COMM ERROR

E305: MS-BARCODE COMM ERROR

Replace the bar code reader with a new
ZK-821V Bar Code Reader failure.
one.

2. TROUBLESHOOTING
Maintenance Information When the following messages appears on the screen, press the specified key on the
USER MAINTENANCE screen and follow the procedure in the operator’s manual.
Keys on the USER MAINTENANCE

Message
screen to be pressed
Check filters.
Check sample cup.
FILTERS & BATHS & CUPS
Check baths.
Check waste cups.
Check pump tubes. PUMP TUBES
Check manual sampling nozzle. MANUAL SAMPLING NOZZLE
Check closed mode sampling nozzle. CAP PIERCE NOZZLE
Check manual rinse unit. MANUAL RINSE UNIT
CLOSED MODE SAMPLING NOZZLE
Check closed mode rinse unit.
& RINSE UNIT

2. TROUBLESHOOTING
Solving Problems from Flags
The flags are displayed when the measurement result is below or above the
threshold for each parameter. When these flags are displayed, the sample should be
checked by counting under microscope. When this measurement result does not
match with the result from the hematology analyzer measurement, and when the
same flags often appears on the hematology analyzer, adjustment on the
hematology analyzer may not be correct or the hematology analyzer may be
damaged. This section describes the possible causes and countermeasures for each
flags.
Blasts and Immature Granulocyte

Blasts appear at the upper area on the right scattergram.
Immature Granulocyte appears at the upper area on the center scattergram.
There are juvenile cells in the
Check the sample by counting under microscope.
FS gain is high and many scatters
appear in the Blasts and Immature Fine gain must be adjusted.
Gr area.
CV of scattergram is out of range Adjust gain. Refer to the “Measuring Polymer
and many scatters appear in the Gain must be adjusted. Microsphere Suspensions for Checking CV”
Blasts and Immature Gr. section.
Left Shift
Left Shift appears at the division line between MO and NE on the left scattergram.
There are many band cells in
Specific characteristics of the the sample.
FL gain is not appropriate and
MO and NE scattergrams are Fine gain must be adjusted.
cross talking
CV of scattergram is out of range Adjust gain. Refer to the “Measuring Polymer
and many scatters appear in the Gain must be adjusted. Microsphere Suspensions for Checking CV”
Left Shift area. section.

2. TROUBLESHOOTING
Atypical Lymphocytes
Atypical Lymphocytes appear in the LY area on the left scattergram.
Atypical lymphocytes in the
FS gain is low and LY and MO Adjust gain. Refer to the “Checking Scattergram
scattergrams are cross talking Procedure” section.
FS gain is high and LY and ghosts Adjust gain. Refer to the “Checking Scattergram
are cross talking Procedure” section.
The environment temperature The environment temperature must be within 15 to
Too many ghosts.
is out of range. 30°C.
! appears on the right of WBC measured value

! may be displayed on the right of WBC value when the RBC ghosts affecting
WBC measurement. During counting, the hematology analyzer detects the RBC
pulses which are under the threshold for WBC.
RBC membrane resistance is high and Dilute the sample using capillary mode and measure
Specific characteristics there are many RBC ghosts. again or measure using microsope.
of the sample.
PLT aggregation. Measure under microscope.
Environmental condition Diluent temperature is low. Environment temperature must be from 15 to 30°C.
is not appropriate. Specified reagents are not used. Only use the specified reagents.
Influence from other
Background noise is high. Refer to the “Background Noise Problems” section.
malfunctions.
WBC sensitivity and threshold settings
WBC sensitivity must be 5 and threshold 4.
are inappropriate.
WBC aperture cap is not attached
Attach the WBC aperture cap correctly.
correctly.
WBC aperture is dirty. Clean the WBC aperture.
WBC aperture is damaged. Replace the WBC aperture cap with a new one.
The screw attaching the measurement
Tighten the screw.
bath is loose.
MC front shield cover and MC front
Measuring unit failure. chassis are not insulated (there may be Remove the cause.
leakage).
Internal electrode is loose. Tighten the internal electrode.
Replace the electromagnetic valve 1 of the CBC
Electromagnetic valve of the CBC measuring unit with a new one.
measuring unit has poor insulation. Replace the electromagnetic valve 6 of the CBC
measuring unit with a new one.
UT-7143 PRE AMP Board failure. Replace the board with a new one.
The hemolysing reagent Electromagnetic valve 2 of the MP- Replace the electromagnetic valve 2 of the complex
is not dispensed 820V Complex Pump Unit has a failure. pump unit with a new one.
properly. UT-7155 DRIVER4 Board failure. Replace the board with a new one.

2. TROUBLESHOOTING
! appears on the right of MCHC measured value

! may be displayed on the right of MCHC value when the MCHC value is out of
the normal range (lower than 28.0 or higher than 38.0). MCHC is known to be a
stable factor, and its physiological variation is approximately 3% independent of
the patients.

Specific characteristics of the RBC aggregation. Measure under microscope.
sample. Sample is hemolysing. Measure under microscope.
Environmental condition is Specified reagents are not used. Only use the specified reagents.
not appropriate. The reagents are out of expiring date. Replace the reagents with new ones.
Calibration of the hematology
Calibration of HGB or HCT is not correct. Calibrate the hematology analyzer.
analyzer is not correct.
C appears on the right of WBC or PLT measured value

When Plt Clumps appears
Platelet aggregation is a phenomenon where some platelets stick together to create
a large aggregated platelet cell. This is because some platelets in the sample
excessively react against anticoagulant. When platelet aggregation occurs, the PLT
value is low and the insufficient hemolysing alarm also occurs because the
aggregated PLT cell is counted as a WBC or RBC ghost. There is a high possibility
that platelet aggregation occurs when heparin is used as an anticoagulant. Also,
when ethylenediaminetetraacetic acid salt (EDTA) is used, platelets aggregation
rarely occurs. This alarm is displayed when there is poor hemolyzation and PLT
value is low.

Specific characteristics of the sample. PLT aggregation. Measure under microscope.
Collect the sample again and use EDTA as
Error due to anticoagulant. Heparin is used as an anticoagulant.
anticoagulant.
When Neutrophilia
Neutropenia
Lymphocytosis
Lymphopenia
Monocytosis
Eosinophilia
Basophilia appears

Calibration for WBC 5 part differentials is
Adjustment and calibration Perform calibration.
not appropriate.
are not appropriate.
Diff gain adjustment is not appropriate. Adjust gain.
Specified reagents are not used. Only use the specified reagents.
Environmental condition is
not appropriate. The environment temperature is out of 15 to The environment temperature must be
30°C. within 15 to 30°C.

2. TROUBLESHOOTING
When Leukocytosis
Leukopenia
Erythrocytosis
Anemia
Anisocytosis
Microcytosis
Macrocytosis
Hypochromia
Thrombocytosis
Thrombocytopenia occurs

Adjustment and calibration Calibration for CBC parameters is not
Perform calibration.
are not appropriate. appropriate.
Specified reagents are not used. Only use the specified reagents.
Environmental condition is
not appropriate. The environment temperature is out of 15 to The environment temperature must be
30°C. within 15 to 30°C.

2. TROUBLESHOOTING
Service Maintenance Screens
This section describes the functions on the SERVICE MAINTENANCE screen.
1. Press the MENU key to display the MENU screen.

Displaying the SERVICE
MAINTENANCE Screen
2. Press the OTHER OPERATIONS key to display the OTHER OPERATION

screen.
3. Press the SERVICE MAINTENANCE key to display the SERVICE

MAINTENANCE screen.

2. TROUBLESHOOTING
CHECK & MAINTENANCE

Screen
For checking unit operation.
MEASURE WBC & RBC

Measures WBC and RBC without diluting operation.
MEASURE WBC
Measures WBC without diluting operation.
MEASURE RBC
Measures RBC without diluting operation.

2. TROUBLESHOOTING
CIRCUIT CHECK
Checks circuit.
PRIME BATHS
Primes WBC and RBC baths.
NOTE
Fluid in the baths must be drained before priming. Otherwise there
may be spillage.
UNIT PRIME
Primes the MC-820V CBC Measuring Unit and electrodes behind the aperture caps
with diluent for adjusting electrode voltage of the CBC measuring unit.
NOTE
Before priming the unit, the WBC and RBC measurement baths must
be primed with diluent. Otherwise, air enters through the apertures
and diluent cannot be filled up in the unit.
UNIT CLEAN
Primes the MC-820V CBC Measuring Unit and electrodes behind the aperture caps
with CLEANAC detergent for removing air bubbles in the CBC measuring unit.
NOTE
• Before priming the unit, the WBC and RBC measurement baths must
be primed with diluent. Otherwise, air enters through the apertures
and detergent cannot be filled up in the unit.
• After doing UNIT CLEAN, prime the unit with diluent by doing UNIT
PRIME.
DRAIN BATHS
Drains diluent from the WBC and RBC baths.
REMOVE FLOWCELLS
Drains fluid from the MF-820V Flow Cell Unit. Use this function when replacing
the flow cell unit.
LIQUID TANK PRIME

Primes the liquid tank of the JQ-822V center piping unit with diluent.
BACKUP RAM CHECK

Perform RAM check.

2. TROUBLESHOOTING
SAMPLE UNIT
MAINTENANCE Screen
This screen has the following menu items.

• MS-820V
• MS-821V
• MS-822V
• CLEANING NEEDLE
The above keys have their own operation screen.
MS-820V
For checking the MS-820V Closed Sampler Unit operation.
INITIAL: Initializes the closed sampler unit.

MOVEMENT TEST: Performs closed sampler unit operation.
NOTE
Do not touch anywhere on the hematology analyzer while the unit is in
operation.

2. TROUBLESHOOTING
MS-821V
For checking the MS-821V Open Sampler Unit operation.
INITIAL: Initializes the open sampler unit.

MOVEMENT TEST: Performs open sampler unit operation.
NOTE
Do not touch anywhere on the hematology analyzer while the unit is in
operation.
MS-822V
For checking the MS-822V Table Unit operation and bar code reader operation.
INITIAL: Initializes the table unit.

BAR CODE TEST: Tests the bar code reading for rack position no. 1 to
10. The rack position no. increments each time this
key is pressed. The test result is displayed on the
right part of the screen.
RACK1-50 BAR CODE TEST:Tests the bar code reading for rack position no. 1 to

2. TROUBLESHOOTING
50. The test result is displayed on the right part of

the screen.
BAR CODE READ RATIO TEST: Tests the bar code reading ratio every 100
readings. The test result is displayed on the
upper right part of the screen.
CLEANING NEEDLE
Cleans the cap pierce nozzle.
PUMP UNIT
MAINTENANCE Screen

• MP-520V 5DIFF
• MP-520V WBC
• MP-520V RBC
• MP-820V
• MP-821V
• VALVE & PUMP CHECK
MP-520V 5DIFF, MP-520V WBC, MP-520V RBC

For checking the MP-520V Pump Unit operation.
NOTE
Remove the pump tubes before performing these operations.
Otherwise, waste fluid enters the hematology analyzer during the
reverse operation.

2. TROUBLESHOOTING
INITIAL: Initializes the pump unit.

FRONT: Checks clockwise (drainage) operation.
REVERSE: Checks counterclockwise operation.
MP-820V
For checking the MP-820V Complex Pump Unit operation.
MANUAL MODE ON: Moves the MS-821V Open Sampler Unit to the
sample aspirating position.
DILUTER INITIAL: Initializes the diluter of the complex pump unit.
DILUTER ASPIRATE: The diluter unit of the complex pump unit
performs the aspirating operation.
DILUTER DISPENSE: The diluter unit of the complex pump unit
performs the dispensing operation.
NOTE
Diluent is dispensed in this operation.
Therefore, before doing this operation,
press the MANUAL MODE ON key to

2. TROUBLESHOOTING
move the open sampler unit to the

aspirating position and hold a container
under the open mode sampling nozzle.
When the MANUAL MODE ON key is not
pressed, the diluent is dispensed from
the sampling nozzle of the closed
sampler unit.
MANUAL MODE OFF: Releases the MANUAL MODE ON key and
returns the open sampler unit to the waiting
position.
ASPIRATE LYSING REAGENT: Hemolysing reagent pump unit of the complex
pump unit performs aspirating operation. There
is no aspirating or dispensing fluid.
INITIAL: Initializes the hemolysing reagent pump unit of
the complex pump unit.
After doing the ASPIRATE LYSING
REAGENT, always press this INITIAL key.
There is no fluid being dispensed.
MP-821V
For checking the MP-821V Sample Pump Unit operation.
INITIAL: Initializes the sample pump unit.

ASPIRATE: The sample pump unit performs aspirating operation.
DISPENSE: The sample pump unit performs dispensing operation.

2. TROUBLESHOOTING
VALVE & PUMP CHECK

For checking the MP-520V Pump Units, MP-822V Sheath Pump Unit and
electromagnetic valve operation. This function has two pages. Press the PAGE key
to change pages.
NOTE
Before doing the following operations, check the fluid path with the
Hydraulic System Diagram in Section 1. If there is any mistake in the
operation, fluid may get anywhere in the hematology analyzer and
damage the hematology analyzer.
MP-520V RBC STOP, MP-520V WBC STOP, MP-520V 5DIFF STOP keys in
the PUMP CHECK area
These keys checks the clockwise (drainage), counterclockwise operation of the
pump units. Every time the key is pressed, the key changes from STOP → FRONT
→ REVERSE → STOP.
AIR PUMP OFF key in the PUMP CHECK area

Turns ON or OFF the air pump in the sheath pump unit. When ON, the first sheath
pressure is automatically set at a constant pressure. When OFF, there is no
operation at the decrease of the first sheath pressure. Every time the key is pressed,
the key changes between ON and OFF.
Keys in the VALVE CHECK area

Turns ON or OFF the electromagnetic valve of each unit.
When 2-way electromagnetic valve is OFF, the electromagnetic path is closed.
When 3-way electromagnetic valve is OFF, the electromagnetic path of the NO
(normal open, white joint side) side is open. When ON, the electromagnetic path
of the NC (normal close, black joint side) side is open.
NOTE
Do not keep these keys to ON. Otherwise, the electromagnetic valves
may heat up.

2. TROUBLESHOOTING
CONTINUOUS
MEASUREMENT Screen
CLOSED MODE 10 SAMPLES

Measures the sample set at the rack position no. 1 repeatedly for 10 times. This
function is used in checking reproducibility.
CLOSED MODE 30 SAMPLES

Measures the sample set at the rack position no. 1 repeatedly for 30 times.
RACK 1, 2 40 SAMPLES
Measures the samples set at the rack position no. 1 and 2 repeatedly for 40 times.
The sample at no.1 is repeatedly measured 10 times, then the same sample is
repeatedly measured 10 times again. Then the sample at no. 2 is repeatedly
measured 10 times and then again 10 times.
MANUAL MODE CONT MEAS.

Performs manual mode measurement repeatedly for 50 times.
CLOSED MODE CONT MEAS.

Measures the samples set on the sample rack repeatedly for 10 times for each
sample. When there is only one sample set on the rack, the sample is measured 10
times. When there are 50 samples set on the rack, each sample is measured 10
times (i.e. 500 measurements are performed).

2. TROUBLESHOOTING
MONITOR Screen

• SENSOR MONITOR
• SCI MONITOR
• POS SENSOR MONITOR
SENSOR MONITOR
Displays the following values at real time.
• Manometer voltage of the MC-820V CBC Measuring Unit, electrode voltage and
HGB sensor voltage
• Voltages of the 4-way liquid sensor, liquid tank sensor and sheath tank sensor
• First sheath pressure and second sheath pressure
• Temperatures of the warmer unit and inside chassis
• Calibration switch settings of the MP-820V Complex Pump Unit and MC-820V
CBC Measuring Unit
For changing the above items and normal ranges, refer to “Adjusting Sensor” in
the Section 6 “Adjustment”.

2. TROUBLESHOOTING
SCI MONITOR
Displays the history of internal communication between the UT-7146 MASTER
board, UT-7147 SLAVE CBC board and UT-7148 SLAVE MS board.
POS SENSOR MONITOR

Displays the setting of the position detecting sensor of each unit in real time.

2. TROUBLESHOOTING
10 DATA X-CV Screen Displays the measured result, average value and CV value of the latest 10 samples.
INITIALIZATION MENU
Screen
The following settings can be initialized and data can be deleted on this screen.
• INITIALIZE CALIBRATION
Initializes the calibration coefficients and deletes calibration history data.
• INITIALIZE DIFF GAIN
Initializes the gain settings for WBC 5 part differential parameters and deletes
gain adjustment history.
• INITIALIZE ALL DATA
Deletes measured data, work list data, X-R data, X-R initial value, X-R limits and
XB initial value.

2. TROUBLESHOOTING
• INITIALIZE SETTINGS
Initializes the ID, normal range, recount range, measurement mode, display
format, CBC sensitivity & threshold and their history, MEK-5D sensitivity and
threshold, output format, operator registration, bar code setting and automatic
cleaning time settings. The gain adjustment settings for WBC 5 part differentials
and date and time settings are not initialized.
• OPERATION HISTORY
Deletes operation history.
• ADVANCED SETTINGS
Initializes the ADVANCED SETTINGS, FLAGS SETTINGS, and
MAINTENANCE ALARM settings.
• FACTORY SETTINGS
Initializes all above settings and deletes all data.
ADVANCED SETTINGS
Screen
Special settings which cannot be set on the SETTINGS screen can be set on this
screen.
• MEASUREMENT MODE
Settings concerning sample measurement can be set.
• UNIT SETTING
Movement settings for each unit can be set.
• SCREEN SETTINGS
Display and communication format can be set.
• FLAGS SETTINGS
Threshold for displaying flags can be set.
• MAINTENANCE ALARM
The number of sample counting can be set for displaying prompts for doing
maintenance on the USER MAINTENANCE screen.

2. TROUBLESHOOTING
MEASUREMENT MODE
The following settings can be set.

• PRIME AFTER MEASUREMENT: default setting ON
When set to OFF, before and after procedures for manual measurement (e.g.
priming and cleaning) are not performed. When these procedures are not
performed, the measurement accuracy is badly affected.
• CLEAN AFTER MEASUREMENT: default setting ON
When set to OFF, automatic waste fluid treatment after measurement is not
performed.
• CONTINUE SAMPLE TYPE: default setting OFF
When set to ON, the settings on the READY (MANUAL) screen remain the same
until settings are changed. When set to OFF, the PARAMETERS setting is CBC
+ Diff and SAMPLE TYPE setting is NORMAL every time this screen is
displayed.
• RECOUNT PLT: default setting 100 (× 103 µL)
When the PLT result is below this setting, the sample is automatically recounted.
• HIGHLANDS CORRESPONDENCE: default setting OFF
When the hematology analyzer is used at 1000 m above sea level, the clog alarm
occurs frequently. Set this setting to ON when using the hematology analyzer at
1000 m above sea level. (HIGH ALTITUDE)
• WBC UNIT PRIME: default setting OFF
When set to ON, the measurement sequence is changed and the manometer is
primed after WBC measurement. This setting returns to the default setting when
the hematology analyzer power is turned off and on again. Do not set this setting
to ON because the measurement accuracy will be badly affected.
• CONTINUE 5D MODE: default setting OFF
When measuring the MEK-5D hematology control, the WBC threshold and diff
gain must be changed for measuring the MEK-5D hematology control. For X-R
measurement, these settings are automatically changed to the settings for MEK-
5D hematology control. When CONTINUE 5D MODE is set to ON, all
measurement are performed with the WBC threshold and diff gain settings set for

2. TROUBLESHOOTING
MEK-5D hematology control. The setting returns to OFF when the hematology
analyzer power is turned off and on again. When measuring human blood, this
setting must be set to OFF. Otherwise, the result is not accurate.
• CONTINUE MANUAL MODE: default setting ON
When set to ON, the Manual Mode key only needs to be pressed once and the
samples are measured in manual mode until the CANCEL key on the READY
(MANUAL) screen is pressed. When the manual mode measurement is
cancelled, the cleaning is performed automatically. When set to OFF, cleaning is
performed after every manual mode measurement and the measurement mode
changes to closed mode.
UNIT SETTINGS
The sample tube type and mixing interval for continuous measurement can be set.
• SELECT TEST TUBE: default setting NORMAL

When using KABE or MONOVETTE sample tubes, select KABE &
MONOVETTE. For other sample tubes, select NORMAL. The sensors and
other parts positions must be modified. Refer to the “Modification Procedure for
Other Sample Tubes” section.
• MIXING INTERVAL: default setting 1
This setting sets the mixing interval for measuring one sample repeatedly in
CLOSED MODE 10 SAMPLES, CLOSED MODE 30 SAMPLES and RACK 1,
2 40 SAMPLES measurement on the CONTINUOUS MEASUREMENT screen.
When this setting is 1, the sample is stirred at every measurement. When this
setting is 2, the sample is stirred every other time.

2. TROUBLESHOOTING
SCREEN SETTINGS
_
• XD•CV LIMIT: default setting ON
When set to ON, the measured data which are out of normal range, alarmed data
_
or data with flags are not used in CV calculation in the XD•CV program. When
set to OFF, all measured data are used in the calculation. This setting returns to
ON when the hematology analyzer power is turned off and on again.
• OVERWRITE WORK LIST: default setting NO
When set to NO, the dialog box asking if you want to overwrite the work list data
when the hematology analyzer receives a new work list data. When set to YES,
the work list data is overwritten with the new data without asking.
• RS-232C PC OUTPUT MODE: default setting MEK-8222
Select the RS-232C output format. When PC is selected for OUTPUT TO on the
RS-232C setting on the OUTPUT FORMAT screen, select the number of ID
digits and number of parameters.
FLAGS SETTINGS
Sets the thresholds for displaying flags. There are three screens for flags settings.
When changing settings, consult the physician or lab technician.
To change the setting, select the parameter with the arrow keys, enter the desired
value with the numeric keys and press the Enter key to register the value.
The INITIALIZE FLAGS key initializes all flags settings.

2. TROUBLESHOOTING
• WBC FLAGS 1
Thresholds for flags for high or low WBC can be set.
• WBC FLAGS 2
Thresholds for abnormal cell detecting flags are set. DO NOT CHANGE THE
SETTINGS ON THIS SCREEN. The settings on this screen is automatically set
according to the sensitivity & threshold setting and gain adjustment.

2. TROUBLESHOOTING
• RBC & PLT FLAGS

Thresholds for RBC and PLT flags can be set.
MAINTENANCE ALARM
Sets the interval (the number of counts) for displaying prompts for performing user
maintenance. In the COUNTS column, the number of counts made since the last
maintenance is indicated. In the LIMITS column, you set the number of counts for
displaying the prompts. When changing settings, consult the physician or lab
technician.
Select the item to be changed with the arrow keys, enter the number using the
numeric keys and press the Enter key to register the value.
Press the RESET TARGET COUNTS key to set the number in the COUNTS
column to 0.
Press the DEFAULT SETTINGS to initialize the LIMIT settings.

2. TROUBLESHOOTING
Modification Procedure for Other Sample Tubes
To use S-Monovette or KABE sample tubes, the following prodedure is required for
use of another position sensor (photo-interruptor).
1. Remove the closed sampler unit. Refer to “Removing the Closed Sampler Unit”
in Section 4 “Disassembly and Assembly”.
2. Disconnect the photo-interruptor cable from the socket of the photo-interruptor

and connect the photo-interruptor cable to another photo-interruptor as shown
below to use the S-Monovette or KABE sample tubes.
<Photo-interruptor connection for S-Monovette

<Photo-interruptor connection for standard
or KABE sample tubes>
sample tubes>
3. Select “KABE & MONOBETTE” at the SELECT TEST TUBE on the UNIT
SETTINGS screen. Refer to page 2.59 “UNIT SETTINGS” section.

2. TROUBLESHOOTING
To use S-Monovette sample tube, the following procedure is additionally required.
1. Remove the 2 screws which secure the mixing unit cover to the closed sampler
unit. Remove the mixing unit cover from the closed sampler unit.
2. Remove the 2 screws which secure the roller head to the rotating shaft. Remove
the roller head from the shaft.
3. Attach the Monovette roller head to the shaft with the 2 screws.

2. TROUBLESHOOTING
When the optional ZK-821V bar code reader is installed in the hematology analyzer
and using S-Monovette or HEMOGARD sample tubes is required, perform the
following procedure.
1. Remove the 2 screws which secure the sample tube roller to the rotating shaft of
the bar code reader. Remove the roller from the shaft.
2. Turn the roller upside down and put the roller back on the shaft. Fasten the roller
to the shaft with the 2 screws.

2. TROUBLESHOOTING
<Sample tube roller position for standard sample tubes> <Sample tube roller position for S-Monovette or
HEMOGARD sample tube>
Optional Racks for S- When using the S-Monovette sample tubes, the optional YZ-0251 Monovette rack is
Monovette and KABE required.
sample tubes
Monovette holder Adapter 8222 Mixing collar
When using the KABE sample tubes, the optional YZ-0250 Kabe rack is required.
Adapter 8222 Mixing collar

Section 3 Board/Unit Description
Sub Bath Unit .................................................................................................................... 3.1

CBC Measuring Unit .......................................................................................................... 3.1
Laser Optical Unit .............................................................................................................. 3.1
Complex Pump Unit ........................................................................................................... 3.1
Sample Pump Unit ............................................................................................................. 3.2
Sheath Pump Unit .............................................................................................................. 3.2
Closed Sampler Unit ......................................................................................................... 3.2
Open Sampler Unit ............................................................................................................ 3.2
Table Unit ........................................................................................................................... 3.2
Display Unit ....................................................................................................................... 3.2
MASTER Board ................................................................................................................. 3.3
SLAVE CBC Board ............................................................................................................ 3.3
SLAVE MS Board .............................................................................................................. 3.3

3. BOARD/UNIT DESCRIPTION
The instrument consists of the following units and boards.
Sub Bath Unit

The sub bath unit has two sub baths and motors which rotate the sub baths. The
two sub baths are used for 200 times for WBC/HGB and 40,000 times for RBC/
PLT diluted samples, respectively. The diluted sample in each sub bath is
poured into the measurement bath of the CBC measuring unit when the sub
bath is rotated.
CBC Measuring Unit

The measuring unit has two measurement baths and manometers. Each
measurement bath has an aperture. The aperture size is different for the two
baths (WBC and RBC/PLT). Two electrodes are positioned so that the aperture
is in between them. When a constant current flows between the two electrodes
and blood cells are passing through the aperture, the voltage between the two
electrodes varies in proportion to the cell size (electrical resistance). The
voltage variation signals, such as pulses for passing cells, are obtained until
the constant volume of diluted sample is aspirated. A voltage which is
proportional to the hemoglobin concentration is also obtained. These data are
sent to the SLAVE CBC board.
Laser Optical Unit

The laser optical unit emits a laser beam that is applied to each blood cell in
the diluted and hemolysed sample which passes through the flow cell in a
single file. The cells reflect and scatter the laser according to their size and
shape. The scattered light is collected in three directions, i.e. forward small
angle scattered light component (FS), forward large angle scattered light
component (FL) and orthogonal scattered light component (SD). These
collected light intensities are converted to electrical signals and amplified on
the PRE AMP board.
Complex Pump Unit

The complex pump unit aspirates a whole blood sample and dilutes it 200
times and 40,000 times. This unit dispenses the constant volumes of the diluent
and hemolysing reagent.

Sample Pump Unit

The sample pump unit aspirates the diluent and dispenses it. This unit sends
the diluted and hemolysed sample to the flow cell at 3 to 6 µl/second.
Sheath Pump Unit

The sheath pump unit applies the constant pressure to the diluent and sends it
to the flow cell.
Closed Sampler Unit

The closed sampler unit releases a pressure inside the sample tube with the cap
pierce nozzle. The unit aspirates the sample in the tube and moves the
sampling nozzle to the sub bath or sampling cup so that the aspirated sample is
ready for the measurement. After the sample is aspirated, the rinse unit cleans
blood from the outside of the sampling nozzle.
Open Sampler Unit

The open sampler unit moves the manual sampling nozzle to the sub bath or
measurement bath so that the aspirated sample is ready for the measurement.
After the sample is aspirated, the manual rinse unit cleans blood from the
outside of the sampling nozzle.
Table Unit
The table unit moves a sample tube set in the rack to the stirring position or
blood aspiration position using x-axis and y-axis drive motors.
Display Unit
The display unit displays the numeric and graphic data and operation keys on
the color LCD screen. This unit controls the LED indicators for the status of
the switches on the front panel.

MASTER Board
The MASTER board controls the operations for the measurement with flow
cytometry method and performs the data processing for the user interface as
shown below.
- Control of the electromagnetic valves and motors
- Amplification and count of the pulses obtained from the scattered light
- LCD control
- USB control
- Memory card control
SLAVE CBC Board

The SLAVE CBC board controls the following operations for dilution and
blood cell count with the electrical resistance detection method.
- Control of the electromagnetic valves and motors
- Amplification and count of the pulses obtained from the electrical
resistance detection
- Dilution control
SLAVE MS Board
The SLAVE MS board controls sample transportation, stirring and dispensing such
as control of the electromagnetic valves and motors.

Section 4 Disassembly and
Assembly
Before You Begin ................................................................................................................ 4.1

Warnings and Cautions ........................................................................................... 4.1
Required Tools ......................................................................................................... 4.1
Caution and Notes Related to Valve Joint, Black Screw and Tube Joint in the
Instrument ............................................................................................................... 4.2
Board and Unit Location .......................................................................................... 4.4
Removing the Front, Right Side, Left Side, Top and Rear Covers .................................... 4.5
Removing the Display Unit ................................................................................................ 4.7
Removing the Laser Optical Unit ...................................................................................... 4.8
Removing the Flow Cell Unit ............................................................................................. 4.9
Removing the Closed Sample Unit .................................................................................. 4.10
Removing the Open Sampler Unit .................................................................................... 4.11
Removing the Table Unit ..................................................................................................4.12
Removing the CBC Measuring Unit ................................................................................. 4.13
Removing the Sub Bath Unit ............................................................................................4.14
Removing the Pump Units under the CBC Measuring Unit ............................................. 4.15
Removing the Pump Unit from the Right Side of the Closed Sampler Unit ....................4.16
Removing the Complex Pump Unit .................................................................................. 4.17
Removing the Sample Pump Unit .................................................................................... 4.18
Removing the Sheath Pump Unit ..................................................................................... 4.19
Removing the 4-channel Liquid Sensor Unit ....................................................................4.20
Removing the Power Supply Unit ..................................................................................... 4.21
Removing the SLAVE CBC Board .................................................................................... 4.21
Removing the SLAVE MS Board ......................................................................................4.22
Removing the MASTER Board .........................................................................................4.22
Removing the POWER Board .......................................................................................... 4.23
Removing the DRIVER1 Board ........................................................................................ 4.24
Removing the INTERFACE Board .................................................................................... 4.28

4. DISASSEMBLY AND ASSEMBLY
The procedures in this section tell how to remove, replace and install major
components in the instrument.
Before You Begin
Removing, replacing and installing major components should be done by

qualified service personnel.
Warnings and Cautions

WARNINGS
• To avoid the possibility of injury to yourself or damage to the
instrument, do not install or remove any component or change
switch settings while the power is on and wait 10 minutes before
installing to or removing any component from the instrument after
the power is off.
• To avoid accidental discharge of static electricity which could
damage the instrument components, use a wrist ground strap when
installing or removing any component of the instrument.
• When replacing any parts or units in the instrument, do not touch
the site of the instrument where blood is or may be.
• Wear rubber gloves to prevent infection by blood.
CAUTIONS
• Before connecting or disconnecting any cables, turn off the
instrument and unplug the AC power cord from the instrument.
• Fuses cut off the power when an abnormality occurs in the
instrument. Eliminate the malfunction before replacing the fuse.
Use the correct fuse only. The fuse rating is shown on the holder.
• Removal and replacement of any component in the instrument
should be done by qualified service personnel.
• Use only parts recommended by Nihon Kohden to assure maximum
performance from your instrument.
Required Tools • Anti-static bench mat

• Wrist ground strap
• Phillips screwdriver (insulated type)
• Flat-blade screwdriver (insulated type)
• Tweezers

Caution and Notes Related

CAUTION
to Valve Joint, Black Screw
Valve Joint
and Tube Joint in the
When connecting the valve joint to the electromagnetic valve, turn the
Instrument
valve joint clockwise, using moderate force until the valve joint
comes to a stop. Do not use extreme force to tighten the valve joint
further because this will damage the tip of the valve joint. If the
valve joint is loosely connected, it will leak.
NOTE
Black screw
Black screws are used to fasten the individual units to the chassis of
the instrument to enable the quick removal and replacement of
these units. However, to fasten the pump unit to the chassis, normal
screws are used.
Spring type tube joint

• Spring type tube joints are used in the instrument to prevent over-
tightening of the joints, and to prevent loosening of the joints after
the joints are tightened.
• There are 2 types of spring tube joints, white (inlet side) and black
(outlet side). Each tube joint and its corresponding port in the
instrument are marked with the same color or number to ensure
matching.

No spring type tube joint

The no spring type tube joint consists of the O-ring, tube stopper
and fitting nut. To disconnect the tube from this joint, turn the
fitting nut counterclockwise to loosen the joint and pull the tube
toward you. To reconnect the tube to this joint, insert the tube into
the fitting nut and turn the fitting nut clockwise to fasten it.
O-ring Tube Fitting Tube

stopper nut

Board and Unit Location The following diagrams show the location of the boards and units in the
instrument.
B
D
E C
Index NK Code No. Description

A UT-7146 MASTER board
B UT-7147 SLAVE CBC board
C UT-7148 SLAVE MS board
D UT-7153 DRIVER2 board
E UT-7155 DRIVER4 board
F UT-7160 POWER board
G UT-7161 INTERFACE board
1000
1400 1300
600 400
800
Index NK Code No. Description 1800
400 JQ-823V 4-channel Liquid sensor unit

600 MC-820V CBC Measuring unit
800 MO-820V Laser optical unit
900 MP-520V Pump unit
1000 MP-820V Complex pump unit
1100 MP-821V Sample pump unit
1200 MP-822V Sheath pump unit
1300 MS-820V Closed sampler unit
1400 MS-821V Open sampler unit
1500 MS-822V Table unit
1800 MF-820V Flow cell unit
1100
900
1200
1500
900

Removing the Front, Right Side, Left Side, Top and Rear Covers
1. Turn off the hematology analyzer.
2. Turn the laser key counterclockwise. The laser key is in the laser switch
on the upper right side panel of the hematology analyzer. Remove the
laser key from the laser switch as shown below.
3. Remove the 2 screws which secure the front cover to the hematology
analyzer at the right and left sides as shown below.
Push the front cover inward at the right and left sides and pull the front
cover toward you to remove it.
4. Remove the 4 screws which secure the right side cover to the hematology
analyzer. Slide the right side cover rearward until the hooks of the cover
are unhooked.

5. Remove the 4 screws which secure the left side cover to the hematology
analyzer. Remove the left side cover from the hematology analyzer.
6. Remove the 3 screws which secure the top cover to the hematology
analyzer. Slide the top cover rearward until the hooks of the cover are
unhooked and pull the cover upward.
7. Remove the 12 screws which secure the rear cover to the hematology
analyzer. Hold the rear cover and slowly separate the rear cover from the
hematology analyzer until you disconnect the cable connected to the
INTERFACE board on the rear cover. Remove the rear cover from the
hematology analyzer completely.

Removing the Display Unit
After performing the “Removing the Front, Right Side, Left Side, Top and Rear
Covers” section, perform the following procedure.
1. Remove the 7 screws (as shown in third figure) which secure the display
unit to the chassis at the right side, left side and top of the display unit.
Slightly push the display unit rearward and unhook its inside hook. Pull
the bottom of the display unit upward as shown in first figure and you can
see the 2 cables with the 3 ferrite cores connected to the display unit as
shown in second figure.
2. Remove the 3 ferrite cores and disconnect the 2 cables from the display
unit. Remove the display unit from the hematology analyzer.

Removing the Laser Optical Unit
1. At the MASTER board, disconnect the cable of the laser optical unit as
shown below.
2. At the flow cytometry piping unit, counterclockwise turn the tube joint (as
shown below) which connects the tube to the top of the laser optical unit.
Unfasten the tube from the two tube ties as shown below.
3. At the flow cytometry piping unit, counterclockwise turn the 2 tube joints
which connect each yellow tube to the lower part of the laser optical unit.
4. At the bottom of the laser optical unit, disconnect the transparent tube
which connects to the flow cytometry piping unit as shown below.
5. Remove the 2 spacers and 2 nuts which secure the laser optical unit to the
chassis. Remove the laser optical unit from the hematology analyzer as
shown below.
MASTER board
Tube tie
CAUTION
Since the flow cell unit projects from the bottom of the laser optical
unit, be careful when removing or attaching the laser optical unit.

Removing the Flow Cell Unit
After performing the “Removing the Laser Optical Unit” section, perform the
1. Remove the 4 screws (as shown below) which secure the cover to the base
of the laser optical unit. Remove the cover from the laser optical unit.
2. Remove the screw (as shown below) which secures the flow cell unit to the
laser optical unit. Remove the flow cell unit from the laser optical unit.
Flow cell unit

Removing the Closed Sample Unit
After performing the “Removing the Display Unit” section, perform the
1. At the upper part of the closed sampler unit, turn the 4 tube joints
counterclockwise as shown in the second figure.
2. At the lower part of the closed sampler unit, turn the tube joint
counterclockwise as shown in the second figure.
3. At the DRIVER3 board, disconnect the 2 cables which connect to the

DRIVER3 board of the closed sampler unit.
DRIVER3 board
4. Remove the sample rack from the hematology analyzer as shown below.
5. Remove the 4 screws (as shown below) which secure the closed sampler
unit to the chassis. Remove the closed sampler unit from the hematology
analyzer.
Sample rack

Removing the Open Sampler Unit
1. At the upper part of the closed sampler unit, turn the tube joint
counterclockwise as shown below.
2. At the lower part of the rinse unit of the open sampler unit, turn the 2 tube
joints counterclockwise as shown below.
3. At the DRIVER4 board, disconnect the 2 cables (as shown below) which
connect to the open sampler unit.
4. Remove the 3 screws (as shown below) which secure the open sampler unit
to the chassis. Remove the open sampler unit from the hematology
analyzer.
DRIVER4 board

Removing the Table Unit
analyzer at the right and left sides. Push the front cover inward at the right
and left sides and pull the front cover toward you to remove it.
3. Remove the sample rack from the hematology analyzer.
4. Remove the 3 screws which secure the table unit to the chassis. Slide the
table unit toward you until the cables behind the table unit can be
disconnected.
CAUTION
Strongly pulling the table unit can damage the cables or DRIVER5
board.
5. At the DRIVER5 board of the table unit, disconnect the 2 cables as shown
below.
NOTE
If it is hard to disconnect the cables from the DRIVER5 board,
remove the right side cover from the hematology analyzer so that
you can easily disconnect the cable by using your hand.
Sample rack
DRIVER5 board

Removing the CBC Measuring Unit
4. Remove the screw (as shown below) which secures the measurement bath
cover for WBC to the CBC measuring unit. Remove the measurement bath
cover from the CBC measuring unit. The sample cup which is attached to
the front of the WBC measurement bath appears.
5. Push the lower part of the sample cup leftward and pull the sample cup
downward. At the chassis, turn the tube joint counterclockwise (as shown
above) which connects the tube to the upper part of the sample cup.
6. At the CBC measuring unit, turn the 5 tube joints counterclockwise as

shown above.
connect to the CBC measuring unit.
8. At the CBC measuring unit, disconnect the 2 cables as shown below.

9. Remove the 2 screws which secure the CBC measuring unit to the chassis.
Pull the CBC measuring unit and sub baths toward you and remove the
CBC measuring unit and sub baths from the hematology analyzer.
DRIVER2 board
Removing the Sub Bath Unit
After performing the “Removing the CBC Measuring Unit” section, perform
the following procedure.
connect to the sub bath unit. Unfasten the each cable from the cable tie.
2. Remove the 2 screws (as shown below) which secure the sub bath unit to
the chassis. Remove the sub bath unit from the hematology analyzer.
DRIVER4 board

Removing the Pump Units under the CBC Measuring Unit
connect to each pump unit. Unfasten each cable from the cable tie.
5. At each pump unit, turn the 2 tube joints counterclockwise as shown

below.
6. Remove the 3 screws (as shown below) which secure the pump unit to the
chassis. Remove the pump unit from the hematology analyzer.
CAUTION in Assembling the Hematology Analyzer

Do not lose the square fitting which must be used between the
pump unit and chassis.
DRIVER2 board

Removing the Pump Unit from the Right Side of the Closed
Sampler Unit
After performing the “Removing the Closed Sampler Unit” section, perform
1. At the DRIVER1 board, disconnect the cable (as shown below) which
connects to the pump unit. Unfasten the cable from the cable tie.
2. At the pump unit, turn the 2 tube joints counterclockwise as shown below.
3. Remove the 3 screws (as shown below) which secure the pump unit to the
chassis. Remove the pump unit from the hematology analyzer.
CAUTION in Assembling the Hematology Analyzer

Do not lose the square fitting which must be used between the
pump unit and chassis.
DRIVER1 board

Removing the Complex Pump Unit
1. At the upper part of the closed sampler unit, turn the tube joint
counterclockwise (as shown below) which connects to the complex pump
unit. At the upper part of the warmer unit, turn the 2 tube joints
counterclockwise (as shown below) which connect to the complex pump
unit. At the upper part of the center piping unit, turn the 5 tube
jointscounterclockwise (as shown below) which connect to the complex
pump unit.
connect to the complex pump unit.
3. Remove the 2 screws (as shown below) which secure the complex pump
unit to the chassis. Remove the complex pump unit from the hematology
analyzer.
Center piping unit
Closed sampler unit
Warmer unit
DRIVER4 board

Removing the Sample Pump Unit
are unhooked.
4. At the sample pump unit, turn the 2 tube joints counterclockwise as shown
below.
connect to the sample pump unit.
NOTE
If it is hard to disconnect the cable from the DRIVER1 board, remove
the top cover from the hematology analyzer so that you can easily
disconnect the cable by using your hand.
6. Remove the 2 screws (as shown below) which secure the sample pump unit
to the chassis. Remove the sample pump unit from the hematology
analyzer.
DRIVER1 board

Removing the Sheath Pump Unit
are unhooked.
4. At the sheath pump unit, turn the 3 tube joints counterclockwise as shown
below. Unfasten the tubes from the tube tie.
5. At the SHEATH CONTROL board of the sheath pump unit, disconnect the
cable as shown below.
6. Remove the 2 screws (as shown below) which secure the sheath pump unit
to the chassis. Remove the sheath pump unit from the hematology
analyzer.

Removing the 4-channel Liquid Sensor Unit
1. At the upper part of the 4-channel liquid sensor unit, turn the 4 tube joints
counterclockwise as shown below.
2. At the chassis, turn the 4 tube joints counterclockwise (as shown below)
which connect to the lower part of the 4-channel liquid sensor unit.
NOTE
Do not remove the four tube joints at the lower part of the 4-channel
liquid sensor unit.
3. At the 4WAY LIQUID SENSOR board of the 4-channel liquid sensor unit,
disconnect the 4 cables as shown below.
4. Remove the 2 screws (as shown below) which secure the 4-channel liquid
sensor unit to the chassis. Remove the 4-channel liquid sensor unit from
the hematology analyzer.

Removing the Power Supply Unit
1. At the POWER board, disconnect the 2 cables (as shown below) which
connect to the power supply unit.
2. Remove the 2 screws (as shown below) which secure the power supply unit
to the chassis. Remove the power supply unit from the hematology
analyzer.
POWER board
Removing the SLAVE CBC Board
1. At the SLAVE CBC board, disconnect all the cables as shown below.
2. Remove the 6 screws (as shown below) which secure the SLAVE CBC
board to the chassis. Remove the SLAVE CBC board from the hematology
analyzer.

Removing the SLAVE MS Board
1. At the SLAVE MS board, disconnect all the cables as shown below.
2. Remove the 6 screws (as shown below) which secure the SLAVE MS board
to the chassis. Remove the SLAVE MS board from the hematology
analyzer.
Removing the MASTER Board
1. At the MASTER board, disconnect all the cables as shown below.
2. Remove the 6 screws (as shown below) which secure the MASTER board
to the chassis. Remove the MASTER board from the hematology analyzer.

Removing the POWER Board
1. At the POWER board, disconnect all the cables as shown below.
2. Remove the 4 screws (as shown below) which secure the POWER board to
the chassis. Remove the POWER board from the hematology analyzer.

Removing the DRIVER1 Board
1. At the DRIVER1 board, disconnect all the cables as shown below.
2. Remove the 4 screws (as shown below) which secure the DRIVER1 board
to the flow cytometry piping unit. Remove the DRIVER1 board from the

to the chassis. Remove the DRIVER2 board from the hematology analyzer.

to the closed sampler unit. Remove the DRIVER3 board from the closed
sampler unit.
to the chassis. Remove the DRIVER4 board from the hematology analyzer.

After performing the “Removing the Table Unit” section, perform the
2. Remove the 4 screws (as shown below) which secure the DRIVER5 board to
the table unit. Remove the DRIVER5 board from the table unit.

Removing the INTERFACE Board
1. At the INTERFACE board attached to the rear cover, disconnect the cable
as shown below.
2. Remove the 5 screws (as shown below) which secure the INTERFACE
board to the rear cover. Remove the INTERFACE board from the rear
cover.

Section 5 Socket Pin Assignment
Socket Pin Assignment ...................................................................................................... 5.1

RS-232C Socket ............................................................................................ 5.1
ZK-820V Socket ............................................................................................ 5.1
USB Socket ................................................................................................... 5.1

5. SOCKET PIN ASSIGNMENT
Socket Pin Assignment
CAUTION
Connect only the specified instruments to the connectors or sockets
marked with by following the specified procedure. Otherwise
electrical leakage current may harm the operator.
RS-232C Socket D sub 25 pin (female)

SDBB-25S-SL-LNK (02)
Pin No. Signal Pin No. Signal Pin No. Signal
1 NC 10 NC 19 NC
2 TXD1 11 NC 20 (To pin 6)
3 RXD1 12 NC 21 NC
4 RTS1 13 NC 22 NC
5 CTS1 14 NC 23 NC
6 (To pin 20) 15 NC 24 NC
7 SG 16 NC 25 NC
8 NC 17 NC
9 NC 18 NC
ZK-820V Socket
Pin No. Signal Pin No. Signal Pin No. Signal

1 FG 4 (To pin 6) 7 CTS0
2 TXD0 5 SG 8 RTS0
3 RXD0 6 (To pin 4) 9 VCC
USB Socket
Pin No. Signal

1 NC
2 USB-D+
3 USB-D−
4 ED

Section 6 Adjustment
Adjusting the Flow Cell Position ......................................................................................... 6.1

Adjusting the FS/FL/SD Gain and FS Threshold ............................................................... 6.4
Adjusting Sensors .............................................................................................................. 6.7
SENSOR MONITOR screen .................................................................................... 6.7
How to call up the SENSOR MONITOR screen ............................................ 6.7
CBC Manometer ...................................................................................................... 6.8
WBC manometer upper sensor adjustment ................................................... 6.9
WBC manometer lower sensor adjustment ................................................... 6.9
RBC manometer upper sensor adjustment ................................................... 6.9
RBC manometer lower sensor adjustment .................................................... 6.9
CBC Electrode ....................................................................................................... 6.10
HGB Sensor ........................................................................................................... 6.11
HGB sensor adjustment ............................................................................... 6.11
Calibration Switch .................................................................................................. 6.12
Liquid Sensor ......................................................................................................... 6.13
4-channel liquid sensor unit adjustment ...................................................... 6.15
Pressure Sensor .................................................................................................... 6.19
Temperature Sensor .............................................................................................. 6.20
Setting on the UT-7159 WARMER CONTROL board .................................. 6.21
Calibrating the Touch Screen ........................................................................................... 6.22

6. ADJUSTMENT
Adjusting the Flow Cell Position
The following problems require adjustment of the flow cell position.
• When a blood sample collected from a healthy person is counted, the scattergram
shows that the 5 parts of WBC are not clearly separated.
• When the 7 µm polymer microsphere suspensions are counted in the Particle
Measurement mode, one of the CV values of FS and FL stays in the
following percentage.
CV of FS: Higher than 7%
CV of FL: Higher than 7%
Adjust the flow cell position (rough adjustment of the gain for WBC 5 part
differential measurement) by measuring 7 µm polymer microsphere
suspensions. The DIFF GAIN screen can only be entered when the type of
operator is lab technician or manufacturer.
Observe the instructions on the 7 µm polymer microsphere suspensions manual

to achieve optimum performance and safety.
CAUTION
• Do not swallow the polymer microsphere suspensions. If
swallowed, contact your physician immediately.
• Avoid contact with the mouth and eyes. If the polymer microsphere
suspensions contacts the mouth or eyes, wash thoroughly and
immediately with water, and contact your physician immediately.
1. Remove the flow cell unit window cover on the right side panel by
removing two screws.
2. Loosen the screw fastening the flow cell unit adjustment screw with the
thick hex wrench. Turn the screw counterclockwise.

6. ADJUSTMENT
3. Insert the thin hex wrench into the flow cell adjustment screw.
Flow cell unit adjustment screw
4. Prepare the polymer microsphere suspensions in a sample tube and 10

blood samples of different healthy people within 8 hours after collection.
5. Press the SETTINGS key on the MENU screen to display the SETTINGS
screen.
6. Press the SENSITIVITY THRESHOLD key on the SETTINGS screen to

display the SENS & THRESH screen.
7. Press the DIFF GAIN key to display the DIFF GAIN (ROUGH) screen.
The ideal peak and current gain values are displayed on the screen.

6. ADJUSTMENT
8. Press the Manual mode key on the front panel to enter manual mode, put
the sampling nozzle into the bottom of the sample tube containing the
polymer microsphere suspensions so that the tip of the sampling nozzle
touches the bottom of the sample tube, and press the Manual count switch.
The polymer microsphere suspensions is aspirated and measurement starts.
The result appears on the screen. When the CV of FS and FL are below 7%,
go to step 10.
When the result is not optimum, press the CLEAN FLOW CELLS key to clean
the flow cell unit. After cleaning, measure the polymer microsphere
suspensions again.
When the result is still not optimum, do the next step.
9. Turn the flow cell adjustment screw about 2 or 3° and press the CLEAR
key to redraw the histograms.
The polymer microsphere suspensions measurement lasts for about 25

about 2 to 3 seconds. If adjusting the flow cell unit is not complete within this period,
measure polymer microsphere suspensions again.
If the histograms are narrower than before, turn the flow cell adjustment
screw in the same direction.
If the histograms are wider than before, turn the flow cell adjustment screw
in the opposite direction.
Repeat drawing histograms, adjusting flow cell unit and redrawing

histograms until you obtain the optimum result.
10. Fasten the flow cell unit adjustment screw.
11. Press the AUTO GAIN key to change the current gain to the target gain.

6. ADJUSTMENT
Adjusting the FS/FL/SD Gain and FS Threshold
Before performing this adjustment, check that the CV values of FS and FL meet the
following conditions by measuring the 7 µm polymer microsphere suspensions.
FS ≤ 7%
FL ≤ 7%
Refer to “Adjusting the Flow Cell Position”.
1. Press the ADJUST GAIN (FINE) key to display the DIFF GAIN (FINE)
screen.
The “For fine adjustment, human blood must be measured. Go to READY

screen, measure blood and return to this screen. Go to READY screen?”
message is displayed.
2. Press the YES key and go to the READY screen.
3. Set the 10 human blood samples which were prepared in step 4 in the rack
and measure them. Make sure that “CBC + Diff” is selected in the
“PARAMETERS” box on the READY screen.
The samples can also be measured manually.
5. Press the LOAD NEW SCATTERGRAM key to draw scattergrams of the

6. ADJUSTMENT
sequence number displayed for “NEW SAMPLE”.
6. When the scattergrams are oval in shape and are not touching the division
line, press the ADD TO LIST key to register the data in the ACTUAL PEAK
LIST. It does not matter if the scattergrams are not within their allotted
area.
Check Points • Check that the WBC distribution is in the square ABED
and does not cover the lines AB, BE, ED and DA.
• Check that the WBC distribution is in the square
BCHG and does not cover the lines BC, CH, HG and GB.
• Check that the WBC distribution is in the square DEGF
and does not cover the lines DE, EG, GF and FD.
• Check that the WBC distribution is in the square IJML
and does not cover the lines IJ, JM, ML and LI.
• Check that the WBC distribution is in the triangle OPR
and does not cover the lines OP, PR and RO.
When the scattergrams are not optimum, do not register the data
in the gain list.
Draw scattergrams of all samples you measured in step 14. You

must have at least one data which has been added to the
ACTUAL PEAK LIST.
7. Auto gain adjustment
Press the AUTO GAIN key to change the current gain to the target gain.
Manual gain adjustment

You can also change the gain by using the numeric keys on the screen.
Press the MANUAL GAIN key, use the arrow keys to move the cursor to
the desired item and enter the number in the EDIT GAIN column.
To adjust size (vertical axis of the scattergram), adjust FS GAIN.

6. ADJUSTMENT
To adjust complexity (horizontal axis), adjust FL GAIN.

To adjust granularity (horizontal axis), adjust SD GAIN.
8. Press the REFRESH key to draw scattergrams with the gain entered in the
EDIT GAIN boxes.
9. Press the ADD TO LIST key to register the data in the ACTUAL PEAK
LIST.
10. When the gain is adjusted, go to the READY screen and measure human
blood sample to check that the scattergrams are optimum and no flags are
displayed.

6. ADJUSTMENT
Adjusting Sensors
SENSOR MONITOR screen The SENSOR MONITOR screen displays the real-time outputs of the sensors in
the instrument. The displayed output values tell you whether the sensor is
functioning correctly, out of adjustment, or faulty.
How to call up the SENSOR MONITOR screen

1. READY screen.
2. Press the MENU touch screen key.

(MENU screen appears.)
3. Press the OTHER OPERATIONS touch screen key.

(OTHER OPERATIONS screen appears.)
4. Press the SERVICE MAINTENANCE touch screen key.

(SERVICE MAINTENANCE screen appears)
5. Press the MONITOR touch screen key.
6. Press the SENSOR MONITOR touch screen key.
The SENSOR MONITOR screen appears.

6. ADJUSTMENT
CBC Manometer The output voltages from the sensors attached to the two manometers in the
CBC measuring unit are displayed.
1. WBC UP: Output voltage of the sensor located at the upper position of the
WBC manometer
2. WBC LOW: Output voltage of the sensor located at the lower position of
the WBC manometer
3. RBC UP: Output voltage of the sensor located at the upper position of the
RBC manometer
4. RBC LOW: Output voltage of the sensor located at the lower position of
the RBC manometer
Diluent or Sample Normal Output Voltage

Present 1.5 V or less
Absent 3.5 V or more
RBC manometer
WBC manometer

6. ADJUSTMENT
WBC manometer upper sensor adjustment

• Adjust the sensor output control VR1 until the WBC UP value on the screen
shows 1.5 V or less when there is liquid at the sensor position level in the
manometer.
• Adjust the sensor output control VR1 until the WBC UP value on the screen
shows 3.5 V or more when there is no liquid at the sensor position level in
the manometer.
WBC manometer lower sensor adjustment
• Adjust the sensor output control VR2 until the WBC LOW value on the
screen shows 1.5 V or less when there is liquid at the sensor position level in
the manometer.
• Adjust the sensor output control VR2 until the WBC LOW value on the
screen shows 3.5 V or more when there is no liquid at the sensor position
level in the manometer.
VR1
VR2
RBC manometer upper sensor adjustment

• Adjust the sensor output control VR3 until the RBC UP value on the screen
manometer.
• Adjust the sensor output control VR3 until the RBC UP value on the screen
the manometer.
RBC manometer lower sensor adjustment
• Adjust the sensor output control VR4 until the RBC LOW value on the screen
manometer.
• Adjust the sensor output control VR4 until the RBC LOW value on the screen
the manometer.
VR3
VR4

6. ADJUSTMENT
CBC Electrode The differential voltages between the two electrodes are displayed.
The normal range of the differential voltage of these electrodes is 18.3 V±0.3
V.
RBC electrode WBC electrode

6. ADJUSTMENT
HGB Sensor HGB sensor output voltage is displayed.
ON: Output voltage from the sensor in the HGB measuring block of the CBC
measuring unit when the LED is lit.
The HGB SENSOR ON voltage must be in the range of 3.0 V±0.3 V.
OFF: Output voltage from the sensor in the HGB measuring block of the CBC
measuring unit when the LED is not lit.
HGB measuring block
HGB sensor adjustment

1. Remove the front cover.
2. Remove the HGB measuring block cover.
3. Call up the SENSOR MONITOR screen.
4. Adjust the HGB sensor output control VR until the HGB SENSOR ON
value shows 3.0±0.1 V when the WBC measurement bath is filled with
diluent.
HGB SENSOR ON value

6. ADJUSTMENT
HGB measuring block cover HGB sensor output control VR
Calibration Switch The correction factors of the CBC measuring unit and complex pump unit are
displayed.
MP-820V: Correction factor which is set by the rotary switches of the complex
pump unit.
MC-820V WBC: Correction factor which is set by the rotary switches of the
WBC measuring block of the CBC measuring unit.
MC-820V RBC: Correction factor which is set by the rotary switches of the
RBC measuring block of the CBC measuring unit.
MP-820V
Rotary switches
MC-820V
Rotary switches
Rotary switches

6. ADJUSTMENT
Liquid Sensor The output voltage from each liquid sensor is displayed.
DETERGENT: Voltage detected by the detergent (CLEANAC and

CLEANAC3) detection sensor in the JQ-823V 4-channel liquid sensor
unit.
HEMOLYNAC 3: Voltage detected by the hemolysing reagent
(HEMOLYNAC 3) detection sensor in the 4-channel liquid sensor unit.
HEMOLYNAC 5: Voltage detected by the hemolysing reagent
(HEMOLYNAC 5) detection sensor in the 4-channel liquid sensor unit.
SHEATH DILUENT: Voltage detected by the diluent (ISOTONAC 3)
detection sensor in the 4-channel liquid sensor unit.
JQ-823V
Liquid Normal Output Voltage

Present 1.5 V or less
Absent 3.5 V or more

6. ADJUSTMENT
LIQUID TANK: Voltage detected by the diluent (ISOTONAC 3)

detection sensor on the UT-7171 LIQUID LEVEL SENSOR board in the
JQ-822V center piping unit.
SHEATH TANK: Voltage detected by the diluent (ISOTONAC 3)
detection sensor on the UT-7171 LIQUID LEVEL SENSOR board in the
P-822V sheath pump unit.
JQ-822V
MP-822V
Liquid Normal Output Voltage

Present 3.5 V or more
Absent 1.5 V or less

6. ADJUSTMENT
4-channel liquid sensor unit adjustment

1. Call up the SENSOR MONITOR screen.
2. Adjust the DETERGENT sensor as follows:

a. When there is no detergent in the detergent sensor block, adjust the
DETERGENT sensor output control 1 until the value indicates 3.5 V or
more.
b. When the detergent sensor block is filled with detergent, adjust the
DETERGENT sensor output control 1 until the value indicates 1.5 V or
less
DETERGENT sensor
DETERGENT sensor block
Output control 1

6. ADJUSTMENT
3. Adjust the HEMOLYNAC 3 sensor as follows:

a. When there is no HEMOLYNAC 3 in the HEMOLYNAC 3 sensor block,
adjust the HEMOLYNAC 3 sensor output control 2 until the value
indicates 3.5 V or more.
b. When the detergent sensor block is filled with HEMOLYNAC 3, adjust
the HEMOLYNAC 3 sensor output control 2 until the value indicates
1.5 V or less.
HEMOLYNAC 3 sensor
HEMOLYNAC 3 sensor block
Output control 2

6. ADJUSTMENT
4. Adjust the HEMOLYNAC 5 sensor as follows:

a. When there is no HEMOLYNAC 5 in the HEMOLYNAC 5 sensor block,
indicates 3.5 V or more
b. When the HEMOLYNAC 5 sensor block is filled with HEMOLYNAC 5,
indicates 1.5 V or less
HEMOLYNAC 5 sensor
HEMOLYNAC 5 sensor block
Output control 3

6. ADJUSTMENT
5. Adjust the SHEATH DILUENT sensor as follows:

a. When there is no diluent in the SHEATH DILUENT sensor block,
adjust the SHEATH DILUENT sensor output control 4 until the value
indicates 3.5 V or more.
b. When the SHEATH DILUENT sensor block is filled with diluent,
adjust the SHEATH DILUENT sensor output control 4 until the value
indicates 1.5 V or less.
SHEATH DILUENT sensor
SHEATH DILUENT sensor block
Output control 4

6. ADJUSTMENT
Pressure Sensor The pressure values detected by the pressure sensors in the sheath pump unit are
displayed.
1st PRESSURE: Pressure detected by the first pressure sensor on the UT-
7158 SHEATH CONTROL board in the MP-822V sheath pump unit.
2nd PRESSURE: Pressure detected by the second pressure sensor on the
SHEATH CONTROL board in the sheath pump unit.
MP-822V
The normal range of the first pressure sensor: 130 to 160 kPa
The normal range of the second pressure sensor: 80 to 120 kPa

6. ADJUSTMENT
Temperature Sensor The temperature values from the temperature sensors in the ZY-820V warmer
unit are displayed.
WARMER TEMP: Temperature detected by the temperature sensor on

the heater of the warmer unit.
INTERNAL TEMP: Temperature detected by the temperature sensor on
the WARMER CONTROL board in the warmer unit.

6. ADJUSTMENT
Setting on the UT-7159 WARMER CONTROL board

The WARMER CONTROL board controls the heater by turning it on or off
according to the temperature set by resistance of the thermistor.
The temperature can be set to 30°C, 35°C, 40°C or OFF by the DIP switch
setting as shown below.
DIP switch
DIP switch setting

Set Temperature BIT 1 BIT 2 BIT 3 BIT 4
30°C ON OFF OFF OFF
35°C OFF ON OFF OFF
40°C OFF OFF ON OFF
Heater OFF OFF OFF OFF ON
The DIP switch is set to 40°C at the factory.

6. ADJUSTMENT
Calibrating the Touch Screen
1. Press the Manual mode key while pressing the reset key.
The TOUCH SCREEN CALIBRATION screen appears.
reset key
Manual mode key
2. Touch the cross-point of the + mark at the upper left corner of the LCD.
Immediately after touching the touch point, the instrument generates a pip
tone and the + mark moves to the upper center point of the LCD.

6. ADJUSTMENT
3. Touch the cross-point of the + mark of the LCD whenever the mark moves
to a different position.
4. In the last touch the cross-point of the + mark at the lower right corner of
the LCD. The screen changes to the READY screen which shows you that
the touch screen calibration is completed.
Last point

Section 7 Maintenance
YZ-0296 Consumable Replacement Parts List in Periodic Maintenance Check .............. 7.1
Procedure before Checking, Cleaning or Replacing a Periodic Replacement Part in the
Hematology Analyzer ........................................................................................................ 7.2
Checking, Cleaning or Replacing the Rinse Unit, Sampling Nozzle and Cap Pierce Nozzle
for Closed Mode ................................................................................................................ 7.4
Checking or Replacing the Manual Sampling Nozzle ....................................................... 7.6
Checking and Cleaning the Manual Rinse Unit ................................................................. 7.7
Checking and Cleaning or Replacing Filters ..................................................................... 7.9
Checking and Cleaning the Sub Baths, Measurement Baths and Sample Cup ..............7.10
Cleaning the Aperture ...................................................................................................... 7.11
Checking and Cleaning the Waste Cup ............................................................................ 7.13
Checking and Replacing the Pump Tubes ........................................................................ 7.14
Checking and Cleaning the Mixing Unit ........................................................................... 7.16
Checking or Replacing the Pinch Valve Tube ................................................................... 7.17
Priming after Periodic Maintenance Check, Storage or Transport .................................. 7.18

7. MAINTENANCE
YZ-0296 Consumable Replacement Parts List in Periodic

Maintenance Check
Qty.
Description NK code
(per instrument)
Cap pierce nozzle set, 2 pcs./set YZ-0262 1 pc.
O-ring for cap pierce nozzle 315366 1 pc.
O-ring for closed mode sampling nozzle 295538 1 pc.
O-ring for manual mode sampling nozzle 315366 1 pc.
Pinch valve tube set, 2 tubes/set (one is 5.5 cm long and the YZ-0282 2 tubes
other is 6.0 cm)
Hemoglobin filter set, 10 pcs./set T802 3 pcs.
Filter packing for measurement bath and air trap 2114-082062B 2 pcs.
Filter packing for waste cup 2114-080153A 1 pc.
Pump tube 2114-080599A 3 tubes
O-ring (white) for WBC aperture cap 553198 1 pc.
O-ring (red) for RBC aperture cap 553233 1 pc.
O-ring (black) for WBC and RBC aperture caps 556827 2 pcs.

7. MAINTENANCE
Procedure before Checking, Cleaning or Replacing a Periodic

Replacement Part in the Hematology Analyzer
1. On the READY screen, touch the MENU key. The MENU screen appears.
2. Touch the OTHER OPERATIONS key on the screen. The OTHER

OPERATIONS screen appears.
3. Touch the DRAIN ALL key on the screen. Drain all fluid from the
hematology analyzer according to the instruction on the screen.
4. When the completion message appears on the screen, touch the USER
MAINTENANCE key on the screen. The USER MAINTENANCE screen
appears.
5. Touch the CAP PIERCE NEEDLE key on the screen. Follow the
instructions on the screen to move the cap pierce nozzle to the specified
position for the maintenance check.

7. MAINTENANCE
6. When the completion message appears on the screen, press the reset key
on the front panel.
Reset key
7. Touch the MANUAL SAMPLING NOZZLE key on the screen. Follow the
instructions on the screen to move the manual sampling nozzle to the
specified position for the maintenance check.
8. When the completion message appears on the screen, set the main power
switch on the rear panel to off.
analyzer at the right and left sides. Remove the front cover by pushing the
front cover inward at the right and left sides.
10. Remove the 4 screws which secure the right side panel to the hematology
analyzer. Remove the right side panel from the hematology analyzer.

7. MAINTENANCE
Checking, Cleaning or Replacing the Rinse Unit, Sampling Nozzle

and Cap Pierce Nozzle for Closed Mode
Check and clean the rinse unit for closed mode every 1000 sample counts.
Check and clean the sampling nozzle and cap pierce nozzle for closed mode
every 2000 sample counts.
WARNING
The sampling nozzle and cap pierce nozzle are sharp and sample
blood may have contacted them. Be careful not to prick yourself
with the sampling nozzle or cap pierce nozzle.
1. Pull out the two black plastic pins and remove the transparent cover from
the mixing unit case.
Black plastic pins
Transparent cover
2. Remove the fitting nut (white) under the rinse unit by turning the fitting
nut as shown below.
Rinse unit
Fitting nut
3. Remove the cap pierce nozzle by pulling it down.
O-ring
Cap pierce tube
Cap pierce nozzle
NOTE
• Do not lose the black O-ring attached to the rinse unit.
4. Remove the tube joint from the sampling nozzle and remove the sampling
nozzle by turning and pulling it up as shown below.
Tube joint
Sampling nozzle

7. MAINTENANCE
5. Remove the 3 screws which secure the rinse unit to the sampler unit.
Remove the tube joint from the rinse unit and remove the rinse unit
sampler unit. If the rinse unit has any damage, replace it with a new one.
Sampling nozzle guide

O-ring
Rinse unit
Tube Joint
NOTE
Do not lose the sample tube guide and O-ring of the rinse unit.
6. Soak the rinse unit, fitting nut, sampling nozzle guide and O-ring in
CLEANAC•3 detergent for about 10 minutes.
7. Remove blood from inside the rinse unit and fitting nut with a cotton swab
moistened with CLEANAC•3 detergent.
8. Rinse the rinse unit, fitting nut, sampling nozzle guide and O-ring with
water and dry them.
9. Put the O-ring and sampling nozzle guide back to the rinse unit and attach
the rinse unit to the sampler unit with the 3 screws.
10. Put the sampling nozzle back to the original position. If the sampling
nozzle has any damage, replace it with a new one (YZ-0261: 2 pcs./set).
11. Put the cap pierce nozzle back to the original position and fasten the cap
pierce nozzle with the fitting nut. If the cap pierce nozzle has any damage,
replace it with a new one (YZ-0262: 2 pcs./set).
NOTE in Assembling the Hematology Analyzer

When putting the cap pierce nozzle back to the original position,
check that the tip of the cap pierce nozzle is as shown below from
the front viewpoint. If the tip of the cap pierce nozzle is facing the
wrong direction, blood sample in a Monovette sample tube may not
be measured.
Wrong Correct

7. MAINTENANCE
Checking or Replacing the Manual Sampling Nozzle
WARNING
The sampling nozzle is sharp and sample blood may have contacted
it. Be careful not to pick yourself with the sampling nozzle.
1. If the sampling nozzle needs to be replaced with a new one, remove the
tube joint and the screw which secures the sampling nozzle to the sampler
unit.
CAUTION
Since the screw can be easily dropped inside the hematology
analyzer, carefully remove the screw.
Screw
Tube joint
2. Turn the sampling nozzle 90o as shown below and remove it by pulling it
up.
Sampling nozzle
3. Insert the new sampling nozzle (YZ-0193: 2pcs./set) into the sampling
nozzle guide, attach the tube joint and fasten the nozzle with the screw.
Make sure that the tube is arranged under the tube guide.
Tube guide

7. MAINTENANCE
Checking and Cleaning the Manual Rinse Unit
Check and clean the manual rinse unit every 1000 sample counts.
1. Turn the rinse unit cap as shown below and remove it.
Remove the two tube joints from the rinse unit so that the rinse unit is
removed. Be careful not to lose the O-ring and spacer from the rinse unit.
Rinse unit cap
Rinse unit holder
Rinse unit
2. Remove the O-ring and spacer from the rinse unit. To remove the O-ring,
insert a cotton swab into the rinse unit from the bottom and push out the O-
ring. Spacer
O-ring
3. Soak the rinse unit, rinse unit cap, O-ring and spacer in CLEANAC•3
detergent for about 10 minutes.
4. Remove blood from inside the rinse unit and rinse unit cap with a cotton
swab moistened with CLEANAC•3 detergent.

7. MAINTENANCE
5. Rinse the rinse unit, rinse unit cap, O-ring and spacer with water and dry
them.
6. Remove blood or salt from the area on the sampling nozzle indicated by
the arrow on the left with a gauze or cotton swab moistened with
CLEANAC•3 detergent.
Sampling nozzle
7. Put the O-ring and spacer back to the rinse unit.

Return the rinse unit and rinse unit cap to their original positions.

7. MAINTENANCE
Checking and Cleaning or Replacing Filters
Check and clean the filters every 1000 sample counts. Replace them when
they are clogged or dirty.
1. Turn the 3 tube joints which have the filter counterclockwise until the tube
joints are removed.
2. Remove the filter from each tube. Use tweezers to remove any dust from
the filter. Rinse the filter with tap water to clean it. If it is still dirty,
replace it with a new one.
Filter
3. Put the tube joints back to the original positions by tightly turning the tube
joints clockwise, so that the filter is held in position.
NOTE
• When attaching the tube joint, be careful not to bend or damage the
filter packing at the bottom of the measurement bath, air trap and
waste cup.
• Be careful that there are two different size filter packings. The filter
packing for waste cup is different from the filter packing for
measurement bath and air trap.
• When there is a leakage and there is no scratch or damage to the
circumference of the filter, reattach the tube joint carefully.

7. MAINTENANCE
Checking and Cleaning the Sub Baths, Measurement Baths and

Sample Cup
Check the sub baths, measurement baths and sample cup every 1000 sample
counts.
Clean the measurement baths, sub baths and sample cup when there is any
dried blood or dust on them. You can check the measurement and sub baths
without removing them.
NOTE
Be careful not to damage the sub baths and measurement baths.
The baths are made of special plastic.
Sub baths 1. Check the WBC and RBC sub baths, measurement baths and sample cup. If
any baths or cups have any dried blood or dust, remove and clean them
according to the following procedure.
Tube joints on the
measurement baths
Remove the measurement bath covers by removing each screw.
Measurement baths
Remove the tube joint of the sample cup and remove the sample cup by
pushing it to the left.
Tube joint
Measurement
bath covers 2. Remove the tube joint from each measurement bath as shown below.
Sample cup
Measurement bath screws
CAUTION
Do not lose the filter packing at the bottom of the measurement
bath.
3. Remove dried blood from inside the baths and cups with tweezers.
4. If dried blood remains inside the baths or cups, soak the baths and cups in
CLEANAC•3 detergent for about 10 minutes.
5. Rinse the baths and cups with water. After the baths and cups are dried,
return each bath and each cup to its original position and tighten the
screws. Make sure that the baths are attached firmly, especially the sub
baths.
7. MAINTENANCE
Cleaning the Aperture
For daily cleaning of the aperture, press the Clean key on the front panel.
However, if the “clogged” or “fluid level” message appears frequently or

background noise is high, clean the aperture by the following procedures.
CAUTION
Be careful not to damage the sub bath and the measurement bath
when removing the aperture cap. The baths are made of special
plastic.
After performing the procedure in “Checking and Cleaning the Sub Baths,
Measurement Baths and Sample Cup” section, perform the following
procedure.
1. Place a cloth or tissue paper under your hand and remove the aperture cap by
pulling it towards you.
NOTE
If it is hard to remove the aperture cap, swing it right and left and pull
it toward you.
2. Carefully rinse the aperture cap. Remove all dirt, especially from the inside.
3. Soak the aperture cap in CLEANAC•3 detergent for about an hour.
If clogged dust still remains in the aperture, insert a syringe of

CLEANAC•3 or 10:1 diluted sodium hypochlorite detergent into the concave
of the aperture cap. Aspirate and dispense the detergent by slowly pressing the
syringe’s plunger up and down to remove the dust around the aperture.
Syringe
Concave

7. MAINTENANCE
NOTE
Be careful not to press the syringe with too much force. Too much
force may damage the aperture.
The condition of the aperture can be checked with a 100x microscope.
CAUTION
Handle the aperture with care. It may be damaged if a sharp object
is used to clean it.
4. Rinse the aperture caps with running water and return them to the original
positions.
• There are two different aperture caps which depend on the WBC and RBC
measurement. Be careful not to attach the aperture caps to the wrong
positions.
Attach the WBC aperture cap (white O-ring) to the white side.
Attach the RBC aperture cap (red O-ring) to the red side.
• Check that the red or white O-ring is at the front.
5. Return each bath to its original position and tighten the screws.
Furthermore push in the center of the sub baths. Make sure that the baths
are attached firmly, especially the sub baths.
6. Put the sample cup back to its original position.

Attach the tube joint to the original position.
7. Attach each measurement bath cover to the original position with the
screw.

7. MAINTENANCE
Checking and Cleaning the Waste Cup

Check the waste cup every 1000 sample counts.
Clean the waste cup when there is any dried blood or dust on it.
1. Check the waste cup. If the waste cup has any dried blood or dust, remove
and clean it according to the following procedures.
Waste cup
Screws
Tube joint
2. Remove the tube joint from the waste cup and remove the waste cup by
removing the two screws.
3. Remove dried blood from inside the waste cup with tweezers.
4. If dried blood ramains inside the waste cup, soak the cup in CLEANAC•3
detergent for about 10 minutes.
5. Rinse the cup with water. After the cup is dried, return the cup to its
original position and tighten the screws.

7. MAINTENANCE
Checking and Replacing the Pump Tubes

Replace the pump tubes every 5000 sample counts.
1. Check the pump tubes for leaks. There are three pump tubes. If you find
leakage, replace the pump tube with a new one according to the following
procedure.
2. Pull out the white tube joint from the tube holder, pull out the pump tube
from the tube guide by turning the pump rotator counterclockwise and
pull out the black tube joint from the tube holder.
Black tube joint

Tube holder
Tube guide
Pump tube
Pump rotator
White tube joint
3. Remove the white and black tube joints and replace the pump tube with a
new one.
4. Put the white tube joint back to the original position, push the pump tube
into the tube guide by turning the rotator counterclockwise and put the
black tube joint to the original position. Make sure that the black parts of
the tube are held properly by the tube holders.
Tube holder
Black part
of the tube
Tube guide
Black part of
the tube

7. MAINTENANCE
NOTE
• Do not damage the pump tube with the tube guide.
• Turn the rotator counterclockwise to put the pump tube into the
tube guide. Otherwise, the pump tube can be disconnected from the
black part of the tube by the applied positive pressure.
• Put back the pump tube properly. If the pump tube has slack, it will
be damaged by the tube guide.
• Make sure that the black parts of the tube are held properly by the
tube holders. Otherwise the pump tube will be scraped by the pump
rotator and the life of the pump tube will be shortened.

7. MAINTENANCE
Checking and Cleaning the Mixing Unit

Check and clean the mixing unit every week.
1. Clean the mixing unit with a cotton swab moistened with water.
2. Remove dried blood around the mixing unit with a cotton swab moistened
with water.

7. MAINTENANCE
Checking or Replacing the Pinch Valve Tube

1. Remove the pinch valve tube from each pinch valve attached to the flow
cytometry piping unit. Disconnect the pinch valve tube from the tube
joints at the ends of the pinch valve tube.
55 mm long pinch valve tube
NO NC
Cross sectional view
60 mm long pinch valve tube
2. Check that the pinch valve tube is not collapsed and has no fluid leak.
3. Replace the pinch valve tube with a new one if it is collapsed or leaks.
Refer to “Consumable Replacement Parts List in Periodic Maintenance
Check”.
CAUTION
• When attaching the pinch valve tube to the pinch valve, set the tube
to the “NC” side as shown above.
• Do not twist the pinch valve tube when attaching it to the pinch
valve.
• There are two different length pinch valve tubes. Make sure that one
is 55 mm long and the other is 60 mm long.

7. MAINTENANCE
Priming after Periodic Maintenance Check, Storage or Transport

Perform the following procedure to use the hematology analyzer after any
fluid is completely drained from the fluid path, e.g. after the periodic
maintenance check, storage or transport.
1. Turn on the hematology analyzer.
2. Touch the following keys on the screen.

MENU key on the READY screen
OTHER OPERATION key on the MENU screen
PRIME ON INSTALLATION key on the OTHER OPERATION screen
After you touch the PRIME ON INSTALLATION key, the following

message appears on the screen.
3. Touch the “YES” key on the screen. Priming starts.

If you touch the “NO” key on the screen, the screen returns to the OTHER
OPERATIONS screen.
When the priming operation is complete, the screen returns to the READY
screen.

Section 8 Replaceable Parts List
General .............................................................................................................................. 8.1

Unit Location ..................................................................................................................... 8.2
Board Location .................................................................................................................. 8.4
Exploded View-1 .................................................................................................................. 8.6
Exploded View-2 .................................................................................................................. 8.8
Exploded View-3 ................................................................................................................ 8.10

8. REPLACEABLE PARTS LIST
General
When ordering parts or accessories from your nearest Nihon Kohden

Corporation distributor, please quote the NK code number and part name
which are listed in this service manual, and the name or model of the unit in
which the required part is located. This will help us to promptly attend to your
needs. Always use Nihon Kohden parts and accessories to assure maximum
performance from your instrument.

Unit Location


1000
1400 1300
600 400
800
1800
1100
900
1200
1500
900

Board Location


B
D
E C

Exploded View-1

1600 PV-820V Display unit
SC-820VJ
2000 Power supply unit
SC-820VK
16 374426 Emergency switch LP2S-17S-889

MEK-8222
A
G
F
C
16
B
2000
E
D
1600

Exploded View-2

7 532639 Pinch valve tube (7.5 m long)
8 6113-042788A Sampling cup


Exploded View-3

500 MB-820V Sub bath unit


Section 9 Demonstration Guide
Introduction ......................................................................................................................... 9.1

Demonstration Outline ............................................................................................... 9.1
Required Items for Demonstration ............................................................................. 9.2
Preparing the Hematology Analyzer .................................................................................... 9.4
Installation Flowchart ................................................................................................ 9.4
Connecting Tubes ...................................................................................................... 9.4
Connecting the Power Cord and Grounding the Hematology Analyzer ....................... 9.6
Connecting the Power Cord ............................................................................. 9.6
Equipotential Grounding .................................................................................. 9.6
Turning the Laser Switch On ..................................................................................... 9.6
Turning On the Power ................................................................................................ 9.7
Setting Date & Time and Priming the Hematology Analyzer ................................................ 9.8
Setting the Date and Time ......................................................................................... 9.8
Priming the Fluid Path ............................................................................................... 9.9
Adjusting Gain and Measuring Background Noise ............................................................. 9.10
Flowchart ................................................................................................................ 9.10
Reference: Principle of Differentiating WBC ............................................................ 9.10
Adjusting Gain in Rough Mode ................................................................................ 9.11
Adjusting Gain in Rough Mode and Measuring Polymer
Microsphere Suspensions .............................................................................. 9.11
Adjusting the Flow Cell Unit Position ............................................................. 9.12
Measuring Background Noise .................................................................................. 9.14
Adjusting Gain in Fine Mode ................................................................................... 9.15
Checking the Gain Adjustment ................................................................................ 9.20
Calibrating the Hematology Analyzer ................................................................................. 9.22
Procedure Flowchart ............................................................................................... 9.22
Changing the Operator ............................................................................................ 9.22
Calibrating CBC Parameters .................................................................................... 9.23
Checking the Calibration Coefficients for WBC 5 Part Differential Parameters ........ 9.26
Changing the Operator Back to a LAB TECHNICIAN .............................................. 9.26
Checking the Data ............................................................................................................. 9.27
Check with MEK-5D Hematology Control ................................................................ 9.27
Check with Venous Blood Samples of a Healthy Person ......................................... 9.28
Interference Substances ................................................................................................... 9.30

Storing and Transporting the Hematology Analyzer ........................................................... 9.33
About the Optional ZK-821V/VG Bar Code Reader ............................................................ 9.35
Checking the Bar Code Reader Function ................................................................. 9.35
Using Bar Codes ..................................................................................................... 9.36

9. Demonstration Guide
Introduction
This section provides an installation and setup flow chart for a demonstration of the
MEK-8222J/K hematology analyzer.
The preparation will take approximately 2 hours.
Demonstration Outline Take the following steps to install and set up the hematology analyzer for
demonstration.
1. Prepare the hematology analyzer before turning the power on.

a) Connect the tubes.
b) Connect the power cord and ground the hematology analyzer.
c) Turn the laser switch on the right side panel to ON for WBC 5 part
differential measurement.
d) Turn on the power.
2. Set the settings and prime the hematology analyzer.

a) Check and set the date and time settings.
b) Prime the hematology analyzer (PRIME ON INSTALLATION).
3. Adjust the gain and measure background noise

a) Adjust the gain for WBC 5 part differential parameters in rough mode using
polymer microsphere suspensions.
b) Measure background noise for WBC, RBC, HGB, PLT and TOC parameters.
c) Adjust the gain for WBC 5 part differential parameters in fine mode using
10 venous blood samples from different healthy people within 8 hours after
collection.
d) Check the actual peak list for all 10 samples to see if the gain is adjusted
correctly. Make sure that the average peak of MO is optimum.
4. Calibrate the hematology analyzer.

a) Perform the calibration for CBC parameters (WBC, RBC, HGB, MCV, PLT,
RDW, MPV) using the MEK-3DN hematology control as a calibrator. This
calibration is performed on the SERVICE MAINTENANCE screen.
b) Check that the calibration coefficients for WBC 5 part differential
parameters (LY%, MO%, EO%, BA%) are 1,000 on the CALIBRATION
screen. In the gain adjustment performed in the previous step, WBC 5 part
differential parameters are already accurately calibrated. Therefore, you
only need to check the calibration coefficient.
5. Check the following data.

_
a) Measure the MEK-5DN/H/L hematology control using the X-R quality
control program in order to check that the obtained data falls within the
acceptable range on the assay sheet attached to the hematology control.
b) Count 20 venous blood samples from different healthy people within 8

hours after collection and make sure that there is no flag such as “Left
Shift”, “Blasts”, “Immature Granulocyte”, or “Atypical Lymphocytes”
displayed.
If the data obtained in the measurement using human blood samples in this
procedure differs from the data obtained using other instruments or at other test
facilities, use the average values of data obtained by the MEK-8222J/K
hematology analyzer and other means for the calibration coefficient.
Required Items for Make sure that you have the following items necessary for the demonstration.
Demonstration These are provided with the hematology analyzer. The number in the parentheses
indicates the necessary quantity.
Waste tubes (2), diluent

Power cord (1) Ground lead (1) tubes (2) detergent tube (1) 18 L tube assy (1)
Cleanac tube assy (2) 18 L cap (3) Hemolynac3 cap (2) Hemolynac3 tube assy (1)
Detergent tube for

Hemolynac5 tube assy (1) CLEANAC•3 (1) Sample rack (1) Laser key (1)
Hex wrench, thin (1) Hex wrench, thick (1) Screwdriver (1) Manuals (1 set)

The following items are also required.
• Waste container and tube assembly. The optional YZ-0248 waste container kit is
available from Nihon Kohden.
• Diluent ISOTONAC•3, MEK-640
• Detergent CLEANAC, MEK-520
• Detergent CLEANAC•3, MEK-620
• Hemolysing reagent HEMOLYNAC•3, MEK-660, or HEMOLYNAC•3N, MEK-
680
• Hemolysing reagent HEMOLYNAC•5, MEK-910
• 7 µm polymer microsphere suspensions, YZ-0194 (supply code no. T905)
• Hematology control, MEK-3DN
• Hematology control, MEK-5DN/H/L
• Sample tubes
• Wiping cloth (tissue papers)
• Rubber or latex gloves
• Distilled water
• Cleaning bottle kit, YZ-0252
• Fixing tape for sample unit and rack table
• 20 blood samples from different healthy people

Preparing the Hematology Analyzer
Installation Flowchart 1. Connect the diluent, detergent, hemolysing reagent and waste containers to the
2. Connect the power cord and perform grounding, if necessary.
3. Turn the laser switch on the right side panel to on for WBC 5 part differential
measurement.
4. Turn on the power of the hematology analyzer.
Connecting Tubes Connect the following reagent bottles and waste container.
• Waste container (the optional YZ-0248 waste container kit is used in this
description)
• Diluent ISOTONAC•3
• Detergent CLEANAC•3
• Detergent CLEANAC
• Hemolysing reagent HEMOLYNAC•3 or HEMOLYNAC•3N
• Hemolysing reagent HEMOLYNAC•5
Tubes and caps necessary for connecting the reagents and container and the inlets
and outlets to which these reagents and container are to be connected are indicated
in the following illustration and table. For details, refer to the operator’s manual.
CAUTION
Avoid reagents or waste fluid contact with the skin. If it contacts the
skin or eyes or is swallowed, wash thoroughly with water and see a
physician immediately.
NOTE
• Be careful not to let dust get in the hemolysing reagent, diluent or
detergent.
• When using the diluent container, detergent container or waste
container, follow the instructions on each package.
• Place the diluent and detergent containers at the same level as the
• Do not squeeze or bend the tubes. Otherwise the hematology
analyzer may be damaged.
• If necessary, cut the tube to an appropriate length if it does not fit.
• Only use the specified detergent tubes for the detergent.

• When using HEMOLYNAC•3N reagent after using HEMOLYNAC•3

reagent, calibrate the hematology analyzer after changing the
reagent. Otherwise the hemoglobin concentration decreases.
• When using HEMOLYNAC•3N reagent, attach the HEMO3N label
provided with the hematology analyzer to cover the HEMO3 label at
the HEMO3 inlet.
Inlet/Outlet on the Hematology

Reagent/bottle Cap Tube/tube assy
Analyzer
Waste container Cap provided with Waste tube 2 Waste tubes WASTE
the waste container assy (marked red) outlets F
Diluent 18 L cap 18 L tube 2 Diluent tubes ISO3
ISOTONAC•3 assy (marked blue) inlets A A
Detergent 18 L cap Cleanac Detergent tube CLN3
B
CLEANAC•3 tube assy (marked white) inlet C
Detergent 18 L cap Cleanac Detergent tube CLN C
CLEANAC tube assy (marked green) inlet B D
Hemolysing reagent Hemolynac3 cap Hemolynac3 tube assy HEMO3 E
HEMOLYNAC•3 or inlet D F
HEMOLYNAC•3N
Hemolysing reagent Hemolynac3 cap Hemolynac5 tube assy HEMO5
HEMOLYNAC•5 inlet E

Connecting the Power Connecting the Power Cord

Cord and Grounding the
Hematology Analyzer CAUTION
Only use the provided power cord. Using other power cords may result
in electrical shock or other injury to the operator.
Connect the provided power cord to the AC SOURCE socket on the rear panel and
plug the cord into a 3-prong AC outlet.
Equipotential Grounding
WARNING
For operator safety, equipotential grounding of all instruments must be
performed. Consult with a qualified biomedical engineer.
When more than one electrical instrument is used, there may be electrical potential
difference between the instruments. The potential difference between the
instruments may cause current to flow to the patient connected to the instruments,
resulting in electrical shock (micro shock).
Always perform equipotential grounding when required. It is often required in the

operating room, ICU room, CCU room, cardiac catheterization room and X-ray
room. Consult with a biomedical engineer to determine if it is required.
When equipotential grounding is required, connect the equipotential ground

terminal on the rear panel of the hematology analyzer to the equipotential ground
terminal on the wall (equipotential grounding system) with the equipotential
grounding lead (potential equalization conductor).
Turning the Laser Switch 1. Remove the laser key from the right side panel.
On
2. Insert the laser key into the laser switch on the right side panel.
3. Turn the laser switch to ON.
NOTE
When the laser switch is off, the "Laser switch off" message is
displayed when the power is turned on. When the measurement is
performed with the laser switch turned off, CBC can be measured but
there is no WBC 5 part differential scattergram and data.

Turning On the Power 1. Press the main power switch on the rear panel of the hematology analyzer to
on. The main power lamp on the front panel lights.
Always leave the main power on except for storage and transportation of the
2. Press the power key on the front panel to on. The power lamp lights. Cleaning
of the fluid path, priming and circuit self-check are automatically performed.
When the laser switch on the right side panel is turned on, the laser lamp also
lights.
Each reagent check is

performed. When the
tube is disconnected
or out of reagent, the
reagent is highlighted
in red.
Error message display
area
When there is an error, the error message appears on the screen.
After priming operation is completed, the READY screen appears. The

hematology analyzer is ready for counting.
NOTE
When the power is turned on soon after it is turned off, the screen
does not appear. If this happens, turn the power off, wait a few minutes
and turn the power on again.

Setting Date & Time and Priming the Hematology Analyzer
Setting the Date and Time After turning the hematology analyzer power on, check that the date and time
displayed at the upper right of the screen is correct.
Date and time
To change the date and time:
1. Display the DATE & TIME screen.
Press MENU key → SETTINGS key → DATE & TIME key.
2. Select the date display format by pressing the desired format key in the "DATE
FORMAT" box.
3. To set the value:

i) Use the arrow keys to move the cursor to the desired item in the "DATE &
TIME SETTINGS" box.
ii) Enter the correct value with the numeric keys on the screen and press the
Enter key on the screen to register the entered value.
4. Press the OK key to return to the SETTINGS screen. The clock starts
immediately from the new date and time.

Priming the Fluid Path The fluid path inside the hematology analyzer must be cleaned and primed before
performing measurements. Otherwise, a clog or noise alarm may occur during
measurement or the background noise may increase. Always prime the fluid path
when using the hematology analyzer after long term storage.
1. Press the MENU key → OTHER OPERATIONS key → PRIME ON

INSTALLATION key. The "Prime on installation?" message box is displayed.
2. Press the "YES" key to prime the hematology analyzer. Priming is performed
for about 20 minutes.
When priming is complete, the READY screen is displayed.

Adjusting Gain and Measuring Background Noise
Flowchart 1. Adjust the gain for WBC 5 part differential parameters in rough mode using
polymer microsphere suspensions.
2. Measure background noise for WBC, RBC, HGB, PLT and TOC parameters.
3. Adjust the gain for WBC 5 part differential parameters in fine mode using 10
venous blood samples from different healthy people within 8 hours after
collection.
4. Check the actual peak list for all 10 samples to see if the gain is adjusted
correctly. Make sure that the average peak of MO is optimum.
Reference: Principle of The hematology analyzer uses the light scatter technique to differentiate WBC into
Differentiating WBC neutrophil, lymphocyte, monocyte, eosinophil and basophil counts.
The diluted blood sample is injected into the flow cell. Blood cells pass through
the sensing zone one by one. A laser beam through the sensing zone is scattered by
the passing cells and the scattered light is detected. The angle and intensity of
scattered light indicates the volume and characteristics of the cell. From this, WBC
is differentiated into 5 parts.
In gain adjustment, you adjust the following.

• FS (forward small angle scatter)
• FL (forward large angle scatter)
• SD (side scatter)
From the forward small angle scatter, the size of the cell is detected.
From the forward large angle scatter, the complexity of the cell is detected.
From the side scatter, the granularity of the cell is detected.
Granularity (SD) Complexity (FL)

Laser
Size (FS)
Diluent Diluent
Sample

Adjusting Gain in Rough Adjust the gain for WBC 5 part differential parameters roughly by measuring 7 µm
Mode polymer microsphere suspensions (YZ-0194, supply code no. T905). When the
result is not optimum, the flow cell unit position must be adjusted.
Adjusting Gain in Rough Mode and Measuring Polymer Microsphere

Suspensions
1. Display the DIFF GAIN (ROUGH) screen.
Press MENU key → SETTINGS key → SENSITIVITY THRESHOLD key →
DIFF GAIN key.
2. Press the Manual mode key on the front panel to enter manual mode.
3. Put the sampling nozzle into the bottom of the sample tube containing the
Sampling nozzle polymer microsphere suspensions so that the tip of the sampling nozzle
Manual count switch
touches the bottom of the sample tube, and press the Manual count switch.
The polymer microsphere suspensions is aspirated and measurement starts.
The result appears on the screen.
Put the sampling

nozzle to this level
4. Check the following values.
• CV of FS and FL are below 7%
• FS PEAK ≅ 61 (which is same as FS IDEAL PEAK)
• FL PEAK ≅ 90 (which is same as FL IDEAL PEAK)

5. When the above values are optimum, go to the next step.
When the CV of FS and FL are above 7%, press the CLEAN FLOW CELL key
on the screen to clean the flow cell unit. After cleaning, measure the polymer
microsphere suspensions again. When the CV of FS and FL are still above 7%,
the flow cell unit must be adjusted. Do the procedure in the “Adjusting the
Flow Cell Unit Position” section.
When the FS PEAK and FL PEAK values are not optimum, press the AUTO
GAIN key to change the current gain to the target gain.
Before auto gain After auto gain
When neither CV nor PEAK values are optimum, first adjust the flow cell unit
position, then adjust gain.
6. Press the OK key to return to the SENSITIVITY THRESHOLD screen.

Adjusting the Flow Cell Unit Position

Adjust the flow cell unit position when the CV of FS and FL are still above 7%
after cleaning the flow cell unit. The polymer microsphere suspensions is
measured for adjusting the flow cell unit position.
1. Remove the flow cell unit window cover on the right side panel by removing
two screws.

2. Loosen the hex screw fastening the flow cell unit adjustment screw with the
thick hex wrench. Turn the hex screw counterclockwise.
Flow cell unit adjustment screw 3. Insert the thin hex wrench into the flow cell adjustment screw.
4. Measure the polymer microsphere suspensions by referring to the “Adjusting

Gain in Rough Mode and Measuring Polymer Microsphere Suspensions”
section.
5. When counting the polymer microsphere suspensions is started, turn the flow
cell adjustment screw about 2 or 3° and press the CLEAR key to redraw the
histograms. When the CLEAR key is pressed, the PARTICLE COUNT is reset
to 0 and starts counting again.
If the histograms are narrower than before, turn the flow cell adjustment screw
in the same direction.
If the histograms are wider than before, turn the flow cell adjustment screw in
the opposite direction.
Repeat pressing the CLEAR key to draw histograms, adjusting flow cell unit
and redrawing histograms until you obtain the narrowest histograms. The
histograms must be cleared each time by pressing the CLEAR key. Otherwise
the previous histograms remain on the screen and the flow cell unit cannot be
adjusted properly.
The polymer microsphere suspensions measurement lasts for about 25 seconds.

Within this period, redrawing histograms can be performed about 3 times. If
adjusting the flow cell unit is not complete within this period, measure polymer
microsphere suspensions again.
6. When you have obtained the narrowest histograms, remove the thin hex wrench
from the flow cell unit adjustment screw. Be careful not to move the flow cell
unit adjustment screw.
7. Fasten the flow cell unit adjustment screw with the thick hex wrench. Turn the
screw clockwise (the screw which was loosened in step 2).
8. Measure the polymer microsphere suspensions and check the CV and PEAK
values. When the CV is still above 7%, repeat the procedure.

9. When the result is optimum, close the flow cell unit window cover with the
screws which were removed in step 1.
10. Press the OK key on the screen to return to the SENSITIVITY THRESHOLD
screen. Cleaning is automatically performed.
Measuring Background To measure the background noise, the diluent is counted in closed mode.
Noise
1. Press the Eject key on the front panel. The rack table slides out.
2. Set a few empty sample tubes in the rack.
3. Press the Start key on the front panel to count the empty samples.
The result is displayed on the screen after measurement. Make sure that the
values are less than or equal to the following values.
WBC: 0.2 (× 103/µL)

RBC: 0.05 (× 106/µL)
HGB: 0.1 (g/dL)
PLT: 10 (× 103/µL)
TOC: 100 (count)
(TOC: total optical count. This parameter only appears in a background
noise measurement.)
Disregard the other parameter values because noise does not affect the other
parameters.
If the values are greater than the above values, check the following items, press
the Clean key to clean the fluid path and recount the diluent.
• The diluent is clean.
• No bubbles in the diluent.
• The apertures and aperture tubes are clean.
• The aperture is firmly attached.
• The measurement baths and sample cup are clean.

Adjusting Gain in Fine Adjust the gain for WBC 5 part differential parameters in fine mode using 10
Mode venous blood samples from different healthy people within 8 hours after collection.
WARNING
Notes about Blood Samples

• When a sample is stored in a cool place, such as a refrigerator, or
when the sample is left for more than 12 hours after collection, it may
affect WBC differential measurement.
• There may be poor hemolyzation in some blood samples when
measured within 30 minutes after collection. In such a case, leave
the sample for more than 30 minutes before measurement.
• Thoroughly mix the samples immediately before measurement.
• If the blood sample is stirred too much so that bubbles are created,
the sample may be hemolyzed.
• If a coagulated sample is counted, the hematology analyzer may be
damaged.
• When the sample has been in the refrigerator for more than a day,
warm the sample to room temperature and thoroughly mix it.
1. Set the 10 human blood samples of different healthy people within 8 hours
after collection in the sample rack and measure them. Make sure that the
“CBC + Diff” is selected in the “PARAMETERS” box on the READY screen.

DIFF GAIN key → ADJUST GAIN (FINE) key.


sequence number displayed for "NEW SAMPLE".
The sequence number displayed on the screen (NEW SAMPLE SEQ#) is of the
latest measurement. When the scattergrams of this sequence number are
drawn, the previous number appears in the SEQ# box. You can select any
sequence number in ENABLE SEQ# by using the numeric keys on the screen.
The SEQ# is the number automatically attached to each measurement by the

hematology analyzer. It is different from the ID. The SEQ# can be checked on
the DATA screen.
The NEW SAMPLE SEQ# appears blank when there is no measurement

performed after adjusting gain in rough mode.
4. When the scattergrams are optimum, press the ADD TO LIST key to register
the data in the ACTUAL PEAK LIST. It does not matter if the scattergrams are
not within their allotted area.
Check Points
• On the left scattergram (FL-FS scattergram), the NE, LY and MO
scattergrams are oval in shape.
• The NE, LY and MO scattergrams are separate from each other.
• The NE, LY and MO scattergrams appear in their correct position in relation
to each other.
If there are too many scattergrams displayed in the upper area on the
scattergrams, the sample may be immature granulocytes or blasts.
When the scattergrams are not optimum, do not register the data in the gain list.
Draw scattergrams of all samples you measured in step 1. You must have at
least one data which has been added to the ACTUAL PEAK LIST.

Optimum scattergrams for automatic gain adjustment
Scattergrams which cannot be used for automatic gain adjustment 1 (FS GAIN
too high)
In this case, adjust the gain in rough mode again.
Scattergrams which cannot be used for automatic gain adjustment 2 (problem

on the sample)
When the following scattergrams appear, it is a specific characteristic of the sample
and is not the hematology analyzer problem. Prepare another sample and adjust the
gain again in fine mode.
a) No NE scattergram, and MO and LY are not separated (sample of a leukemia

patient)

b) MO scattergram overlaps the NE scattergram (Immature granulocyte)
c) NE scattergram is too close to MO scattergram (Left shift or a blood sample

which has been left for more than 8 hours)

5. When the AVERAGE ACTUAL PEAK and the IDEAL PEAK in the MO
PEAK table at the upper right screen differ, the gain must be adjusted. Press
the AUTO GAIN key on the DIFF GAIN (FINE) screen to change the
CURRENT GAIN to that of the TARGET GAIN.
MO PEAK table
Manual gain adjustment

You can also change the gain by using the numeric keys on the screen. Press
the MANUAL GAIN key, use the arrow keys to move the cursor to the desired
item and enter the number in the EDIT GAIN column.
To adjust size (vertical axis of the scattergram), adjust FS GAIN.
To adjust complexity (horizontal axis), adjust FL GAIN.
To adjust granularity (horizontal axis), adjust SD GAIN.
When the gain is changed, the measured data which were used in the gain
adjustment can no longer be used and their SEQ# are deleted from the
ENABLE SEQ#. When continuing gain adjustment, count the human blood
samples again.
6. Press the REFRESH key to draw scattergrams with the gain entered in the
EDIT GAIN boxes and the CURRENT GAIN values which appeared when the
DIFF GAIN (FINE) screen was first displayed.
NOTE
The CURRENT GAIN values used in this step are not the values now
displayed but the values which appeared when the DIFF GAIN (FINE)
screen was first displayed.

Checking the Gain 1. Set the 10 human blood samples of different healthy people within 8 hours
Adjustment after collection in the sample rack and measure them. Make sure that “CBC +
Diff” is selected in the “PARAMETERS” box on the READY screen.

DIFF GAIN key → ADJUST GAIN (FINE) key.

sequence number displayed for "NEW SAMPLE". Draw scattergrams of all 10
measured data.
4. Check the scattergrams and MO PEAK values. To check the MO PEAK

values, press the LIST key to display the ACTUAL PEAK LIST screen.
Check Points for Scattergrams

• The scattergrams are oval in shape and are not touching the division line.
• The scattergrams are displayed in the MO, LY and NE area on the left
scattergram (FL-FS scattergram).
• There are not many scattergrams displayed in the upper area on the center
and right scattergrams (SD-FS scattergrams).
Check Points for MO PEAK

The AVERAGE PEAK value and IDEAL PEAK value are the same on the
ACTUAL PEAK LIST screen.
MO PEAK value optimum range
MO FS PEAK = 186 to 192
MO FL PEAK = 47 to 51
MO SD PEAK = 21 to 23

5. When the scattergrams and MO PEAK values are optimum, the gain is adjusted
correctly. If not, adjust the gain again in fine mode. When the sample used in
the adjustment is not human blood or when the gain needs to be changed
greatly, gain may not be adjusted in a single adjustment.

Calibrating the Hematology Analyzer
Procedure Flowchart NOTE

The calibration procedure described in this section differs from the
procedure described in the operator’s manual. The average values of
multiple measurement are used for higher accuracy.
1. Change the operator to MANUFACTURER.
2. Perform the calibration for CBC parameters (WBC, RBC, HGB, HCT, PLT,
RDW, MPV) using MEK-3DN hematology control as a calibrator. This
calibration is performed on the SERVICE MAINTENANCE screen.
3. Check that the calibration coefficients for WBC 5 part differential parameters
(LY%, MO%, EO%, BA%) are 1,000 on the CALIBRATION screen. In the
gain adjustment performed in the previous section, WBC 5 part differential
parameters are already accurately calibrated. Therefore, you only need to check
the calibration coefficient.
4. Change the operator back to LAB TECHNICIAN.
Changing the Operator To prevent measuring with wrong measurement conditions, there are screens or
functions that can only be entered or changed by an operator who is given the
authority and password. There are three levels for the authority.
MANUFACTURER: For servicing the hematology analyzer. Can enter any screen
and change any settings. The status bar on the screen is
displayed in orange.
LAB TECHNICIAN: For changing settings. Can enter any screen and change any
settings except for the SERVICE MAINTENANCE screen.
The status bar on the screen is displayed in blue.
OTHER: Can change some settings, but mostly can only perform
measurement and view the data and settings. The status bar
on the screen is displayed in blue with a lock.
The operator must be MANUFACTURER to do the procedure described in this

section.
1. Display the OPERATORS & PASSWORDS screen.

Press MENU key → SETTINGS key → OPERATORS & PASSWORDS key.

2. Select FACTORY and press the SELECT OPERATOR key. The password
window is displayed.
Password window
3. Enter the password “4321” using the numeric keys and press the Enter key.
The CURRENT OPERATOR changes to FACTORY.
Calibrating CBC Calibrate the CBC parameters (WBC, RBC, HGB, HCT, PLT, RDW and MPV)
Parameters using MEK-3DN hematology control as a calibrator. This calibrator can be used
when it is within 3 days after opening, stored at 2 to 8°C and handled appropriately
according to its manual. For higher accuracy, use the calibrator soon after its
opening.
HCT is calculated from RBC and MCV, therefore, it is calibrated by calibrating

RBC and MCV.
1. Set a sample tube containing MEK-3DN calibrator in rack position no. 1 of the
sample rack.
2. Display the CONTINUOUS MEASUREMENT screen.

MAINTENANCE key → CONTINUOUS MEASUREMENT key.

3. Press the CLOSED 10 SAMPLES key to count the calibrator 10 times.
4. Display the 10 DATA X-CV screen.

Press the 10 DATA X-CV key on the SERVICE MAINTENANCE screen.
Mean value
5. Calculate the new calibration coefficient for each parameter form the mean
value according to the equation below.
Calibrator assay value

New calibration coefficient = Previous calibration coefficient ×
Mean measured value

Press MENU key → CALIBRATION key → CLOSED key.

7. Enter the new calibration coefficient.

1) Press the WBC key to calibrate WBC, press RBC to calibrate RBC, HGB,
MCV (HCT) and RDW, and press PLT to calibrate PLT and MPV.
2) Use the arrow keys to move the cursor to the calibration coefficient box of
the parameter you want to calibrate.
3) Use the numerical keys on the screen to enter the new calibration
coefficient you calculated in step 5. Press the Enter key to register the
value.
4) Repeat this step to change the calibration coefficient for other parameters.
For calibrating HCT, enter the new calibration coefficient you calculated in
step 5 for MCV in the HCT box.
8. Press the OK key.
9. Count the calibrator which is set in position no. 1 of the rack 10 times
continuously on the CONTINUOUS MEASUREMENT screen (refer to steps 1
to 3).
10. Display the 10 DATA X-CV screen and check that the average value is the
same as the value on the assay sheet provided with the calibrator.
When the values do not match, calibrate again.

Checking the Calibration Check that the calibration coefficients for WBC 5 part differential parameters
Coefficients for WBC 5 Part (LY%, MO%, EO%, BA%) are 1,000 on the CALIBRATION screen. In the gain
Differential Parameters adjustment performed in the previous section, WBC 5 part differential parameters
are already accurately calibrated. Therefore, you only need to check the calibration
coefficient.

Press MENU key → CALIBRATION key → CLOSED key.
2. Check that the calibration coefficients for LY%, MO%, EO% and BA% are
1000.
If not, enter 1000 in the calibration coefficient boxes using the arrow keys,
numeric keys and Enter key on the screen.
Changing the Operator Change the operator back to LAB TECHNICIAN.

Back to a LAB
TECHNICIAN 1. Display the OPERATORS & PASSWORDS screen.
Press MENU key → SETTINGS key → OPERATORS & PASSWORDS key.
2. Select MEK-F and press the SELECT OPERATOR key. The password window
is displayed.
3. Enter the password “8222” using the numeric keys and press the Enter key.
The CURRENT OPERATOR changes back to MEK-F.

Checking the Data
Check the following data to confirm that the gain is adjusted and calibration is
performed properly.
_
a) Measure the MEK-5DN/L/H hematology control using the X-R quality control
program in order to check that the obtained data falls within the acceptable
range on the assay sheet attached to the hematology control.
b) Count 20 venous blood samples from different healthy people within 8 hours
after collection and make sure that there is no flag such as “Left Shift”,
“Blasts”, “Immature Granulocyte”, or “Atypical Lymphocytes” displayed.
_
Check with MEK-5D Measure the MEK-5DN/L/H hematology control using the X-R quality control
Hematology Control program in order to check that the obtained data falls within the acceptable range
on the assay sheet attached to the hematology control.
1. Display the QUALITY CONTROL screen.

Press MENU key → QUALITY CONTROL key.
_ _
2. Press the X-R (NORMAL) key to display the X-R (NORMAL) screen.
3. Set the sample tube containing the MEK-5DN hematology control in rack
position no. 1 and press the Start key on the front panel to count the
hematology control. The hematology control is measured twice.
_
After measurement, the X and R data appear on the screen.

When an alarm or “OVER” occurs in either measurement, there will be no

measurement result.
_
4. Measure the MEK-5DH hematology control on the X-R (HIGH) screen and the
_
MEK-5DL hematology control on the X-R (LOW) screen.
5. Check that the measured data is in the acceptable range on the assay sheet
provided with the hematology control.
When the CBC parameters are outside the range, calibrate the CBC parameters.
When the WBC 5 part differential parameters are outside the range, adjust the
gain in fine mode.
Check with Venous Blood Count 20 venous blood samples from different healthy people within 8 hours after
Samples of a Healthy collection and make sure that there is no flag such as “Left Shift”, “Blasts”,
Person “Immature Granulocyte”, or “Atypical Lymphocytes” displayed.
If the data obtained in the measurement using human blood samples in this
procedure differs from the data obtained using other instruments or at other test
facilities, use the average values of data obtained by the MEK-8222J/K hematology
analyzer and other means for the calibration coefficient.
WARNING

1. Set 20 human blood samples from different healthy people within 8 hours after
collection in the sample rack and measure them in closed mode. Make sure
that the “CBC + Diff” is selected in the “PARAMETERS” box on the READY
screen.
2. Check that the following flags are not displayed on the result.
• Blasts
• Immature Granulocyte
• Left Shift
• Atypical Lymphocytes
If any flags are displayed, adjust the gain in fine mode.
Flag display area

Interference Substances
WBC: Unlysed red cells

In some rare occasions, the RBC in the blood sample may not completely lyse and these non-lysed RBC
may be detected as WBC and cause increase in WBC count.
Multiple myeloma
The precipitation of proteins in multiple myeloma patients may increase the WBC count.
Leukemia
WBC is fragile in leukemia patient and WBC may be destroyed during measurement. These WBC
fragments may also interfere with WBC differential measurement.
Chemotherapy
Cytotoxic and immunosuppressive drugs cause low WBC count.
Cryoglobulins
Cryoglobulin may be increased in patients who are or have myeloma, cancer, leukemia, macroglobulinemia,
lymphoproliferative disorders, metastatic tumors, autoimmune disorders, infections, aneurysm, pregnant,
thromboembolic phenomena, diabetes, etc, which cause increase in WBC, RBC or PLT counts and HGB
concentration. In such cases, warm the blood sample to 37°C in a water bath for 30 minutes and measure
the sample immediately.
RBC: Leukemia
An increase in WBC in leukemia patient causes increase in RBC.
Agglutinated RBC
Agglutinated RBC may decrease RBC count. This can be checked by abnormal MCH and MCHC values
and examination of the stained blood film.
Cold agglutinins
IgM immunoglobulins which are elevated in clod agglutinin disease may decrease RBC and PLT counts and
increase MCV.
HGB: Turbidity of the blood sample
Any physiologic and/or therapeutic factors may increase HGB. In such a case, determine the cause of
turbidity and follow the appropriate method below.
1. Increased WBC
An extreme increase in WBC will cause excessive light scatter. In these cases, measure manually.
Centrifuge the diluted sample and measure the supernatant fluid with a spectrophotometer.
2. Increased lipids
The blood sample may be milky when there is excessive lipid. This may occur with hyperlipidemia,
hyperproteinemia and hyperbilirubinemia. Accurate HGB measurement can be achieved by manual
methods and a plasma blank.
3. Increased turbidity
When RBC are resistant to lysing, turbidity may increase causing increase in HGB. Observe if MCH
and MCHC values are abnormal. HGB result affects MCH and MCHC result.
4. Fetal bloods
The mixing of fetal and maternal blood may increase HGB value.
5. High WBC levels
Turbidity of blood increases and the hemoglobin concentration becomes high if WBC level of the blood
sample is high. MCH and MCHC also become high level.
HCT: Agglutinated RBC

MCV: Agglutinated RBC

Excessive number of large PLT and/or excessively high WBC may affect the MCV value. Check by careful
examination of the stained blood film.
MCH: MCH is determined from HGB and RBC values. Therefore, the limitations for HGB and RBC also affect
MCH value.
MCHC: MCHC is determined from HGB and HCT values. Therefore, the limitations for HGB and HCT also affect
MCHC value.
RDW: RDW is determined from RBC value. Therefore, the limitations for RBC also affect RDW value.
Agglutinated RBC
Agglutinated RBC may decrease RBC count and erroneous RDW. This can be checked by abnormal MCH
and MCHC values and examination of the stained blood film.
PLT: Very small fragments
Very small RBC, RBC fragments and WBC fragments may be the cause in increased PLT count.
Agglutinated RBC
PLT may be trapped in the agglutinated RBC resulting in decrease in PLT. This can be checked by
abnormal MCH and MCHC values and examination of the stained blood film.
Very large PLT
Large PLT may exceed the PLT threshold and may not be counted which results in low PLT count.
Chemotherapy
Cytotoxic and immunosuppressive drugs may increase the fragility of cells which may cause low PLT
count. In such a case, measure manually.
Hemolysis
Hemolyzed specimens contain red cell stroma which may increase PLT count.
Blood anticoagulated with acid-citrate-dextrose may have clumped PLT which may cause decrease in PLT
count.
Agglutinated PLT
Clumped PLT may decrease PLT count and/or increase WBC count. For such sample, collect the sample in
sodium citrate anticoagulant and measure only PLT. The PLT result must be corrected for the sodium
citrate dilution effect.
MPV: Very large PLT
Large PLT may exceed the PLT threshold and not be counted which results in low MPV.
Very small RBC, RBC fragments and WBC fragments may interfere with MPV measurement.
Agglutinated RBC
PLT may be trapped in the agglutinated RBC resulting erroneous MPV. This can be checked by abnormal
Chemotherapy
Cytotoxic and immunosuppressive drugs may affect MPV. In such a case, measure manually.
NOTE
Blood samples collected in EDTA does not maintain stable MPV because platelets swell
depending on the interval after collection and storage temperature.

WBC 5 part differential parameters are derived from the WBC count, therefore, the limitations for WBC also affect these
parameters.
NE and NE%: Excessive eosinophils, metamyelocytes, myelocytes, promyelocytes, blasts and plasma cells may interfere
with an accurate NE count and NE%.
LY and LY%: Erythroblasts, certain parasites and RBC that are resistant to lysis may interfere with an accurate LY count.
MO and MO%: Large lymphocytes, atypical lymphocytes, blasts and excessive number of basophils may interfere with an
accurate MO count.
EO and EO%: Abnormal granules may interfere with an accurate EO count.
BA and BA%: Immature cell, metamyelocytes, myelocytes, promyelocytes, blasts and plasma cells may interfere with an
accurate BA count and BA%.

Storing and Transporting the Hematology Analyzer
WARNING
This procedure must be performed to safely store and transport the
hematology analyzer. There are many expensive units installed in the
hematology analyzer. If there is any malfunction caused from not
doing this procedure, the expense necessary for performing
maintenance for that damage will be charged to you.
When storing and transporting the hematology analyzer is necessary after

demonstrating the hematology analyzer, clean it doing the following procedures.
Clean the inside of the hematology analyzer only with distilled water. After
cleaning, make sure that all distilled water is completely drained from the
If diluent is left inside the hematology analyzer, the inside of the hematology
analyzer will become dirty because of dried diluent crystal or other dust. As a
result, background noise will be increased.
1. Press the Clean key on the front panel to clean the fluid path.
2. Remove the diluent tubes from the ISO3 inlets, the hemolysing reagent tubes
from the HEMO3 (or HEMO3N) and HEMO5 inlets, and the detergent tube
from the CLN3 inlet on the right side panel. Remove the detergent tube from
the CLEANAC container and put this detergent tube into the waste container.
Do not disconnect the waste fluid tubes from the waste outlets or the detergent
tube from the CLN inlet.
CAUTION
Make sure that the tubes are correctly connected.
3. Display the OTHER OPERATIONS screen.

Press the MENU key → OTHER OPERATIONS key.
4. Press the DRAIN ALL key on the OTHER OPERATIONS screen. Check that
the tubes are connected correctly and press the YES key to drain all fluid from
the hematology analyzer.

5. Connect the spare tubes to the ISO3, HEMO3, HEMO 5, CLN and CLN3 inlets
and put the other end of the tubes into the distilled water. (The optional YZ-
0252 Cleaning bottle kit is available for easy setup.)
6. Press the PRIME ON INSTALLATION key on the OTHER OPERATIONS

screen. Press the YES key to prime the hematology analyzer with the distilled
water.
7. Do steps 2 to 4 to drain all fluid from the hematology analyzer again.
8. Press the main power switch on the rear panel to turn the power off. The power
lamp and main power lamp are off.
NOTE
Do not turn the hematology analyzer power off by pressing the power
switch on the front panel. If the power switch is pressed, cleaning is
automatically performed and diluent will be filled in the fluid path.
9. Remove the two screws of the front cover on the side

panels.
10. Pull the front cover toward you. When removing the
cover, push in the sides of the front cover.
11. The cap pierce nozzle for closed mode is inside the waste
cup. Push down the plate as far as it goes and fasten it to
the waste cup with tape.
12. Fasten the sample rack to the table unit with tape.
Plate
13. Reattach the front cover and fasten it with the two screws
which were removed in step 9.
Sample rack
Reference
Waste cup The sampling nozzle for manual mode and cap pierce nozzle
for closed mode are moved to their position for transport after
the DRAIN ALL operation. To move the nozzles to their
transporting position without doing the DRAIN ALL
operation, do the following steps.
1. Change the operator to FACTORY (MANUFACTURER).
2. Press MENU key → OTHER OPERATIONS key →
SERVICE MAINTENANCE key → SAMPLE UNIT
MAINTENANCE key → TRANSPORT POSITION key.
3. Change the operator back to MEK-F.

About the Optional ZK-821V/VG Bar Code Reader
When using the optional ZK-821V/VG bar code reader, the bar code reading can be
checked. To do this, the operator must be MANUFACTURER.
Checking the Bar Code 1. Set an empty sample tube with a bar code label in position no. 1 on the rack.
Reader Function
2. Display the MS-822V MAINTENANCE screen.
MAINTENANCE key → SAMPLE UNIT MAINTENANCE key → MS-822V
key.
Reads the bar code label. The

location number is incremented
every time this key is pressed.
Reads the bar code label of all 50

sample tubes in the rack.
Reads the bar code label at rack

position no. 1 repeatedly 100
times and displays the reading
ratio
Test result is displayed in this area
3. Press the BAR CODE TEST key. The bar code reader reads the bar code of the
sample tube and the reading result is displayed on the screen.
When the bar code reader cannot read the bar code or there is no bar code label
attached to the sample tube, the “NO BAR CODE” message appears on the
screen.
When the sample tube with the bar code label is in another location in the
sample rack, the rack location can be incremented every time the BAR CODE
TEST key is pressed.

When the bar code cannot be read, the angle of the bar code reader position may
need to be adjusted. Do the following procedure. Also, refer to the “Using Bar
Bar code reader
Codes” section.
1. Set the sample tube with the bar code label in the rack position no. 1. Put the
sample tube so that the bar code label is facing toward you.
2. On the MS-822V MAINTENANCE screen, press the BAR CODE READ

RATIO TEST key. The bar code reader reads the bar code 100 times and the
reading ratio (the number of times the bar code is successfully read by the
reader out of 100 attempts) is displayed on the screen.
Loosen this screw
3. Loosen the screw of the bar code reader and adjust the angle of the bar code
reader so that the reading ratio becomes 100.
4. Press the OK key to return to the SAMPLE UNIT MAINTENANCE screen.

The sample rack returns to the initial position.
Using Bar Codes A bar code consists of narrow bars (NB), wide bars (WB), narrow spaces (NS) and
wide spaces (WS). The width of the WB and WS depends on the width of the NB.
NB
WB The ratio between NB and WB is NB:WB = NS:WS = 1:2 to 1.3. Usually, it is
1:2.5.
For the bar code to be read properly by the bar code reader, attach the bar code
label to the sample tube checking the following points.
WS
• The bar code length must be within 60 mm.
NS • NB must be wider than 0.125 mm.
• The left and right margins must be larger than 10 times that of NB.
Within 10 degrees • Attach the bar code label so that the bottom of the label is within 10 mm from the
bottom of the sample tube.
• Attach the bar code label so that it is not more than 10 degrees from vertical as
shown at left.
bar code length
margin margin
Within 10 mm
start character data check digit stop character

NOTE
• An ID may not be read properly by the bar code reader due to poor
printing quality of the label or the label is torn or detached. In such a
case, the ID is automatically assigned by the hematology analyzer.
For such a sample, edit the ID on the EDIT ID screen of the DATA
screen after measurement. Be careful not to mix up such samples.
• When using the ITF bar code type, IDs may be frequently misread by
the bar code reader when compared to the other types of bar codes,
especially when the printing quality of the label is poor. An ID may
be entered even for a sample without a bar code label. Therefore, be
careful not to mix up samples when using ITF bar codes.
An ID of up to 13 digits can be entered. If the bar code has more than 13 digits,
the extra digits are deleted.
If the bar code cannot be read properly by the bar code reader, check the following
points.
• Bar code is dirty or damaged
• Margin on the bar code is too small
• Bar code print is faint
• Bar code is printed in silver or is covered by laminate film
• The printing quality of the bar code is poor (The printing quality is poor
especially when printed on a dot printer or ink jet printer. When printing on such
a printer, NB must be as wide as possible. If the bar code type is JAN or
CODE128, code may not be read properly.)
• The appropriate bar code type or check digit type is not set on the BAR CODE
screen.

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MEK- 8222J SERVICE MANUAL

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Section 1 General .................................................................................. 1C.1

Section 2 Troubleshooting ................................................................... 2C.1

Service Manual MEK-8222 C.1

Operating Condition Check ......................................................................................... 2.2

C.2 Service Manual MEK-8222

A025: WBC bubble 2 ...................................................................................... 2.35

Service Manual MEK-8222 C.3

E302: MASTER-CBC1 COMM ERROR .......................................................... 2.45

C.4 Service Manual MEK-8222

UNIT SETTINGS ........................................................................................... 2.66

Section 3 Board/Unit Description ........................................................ 3C.1

Section 4 Disassembly and Assembly ............................................... 4C.1

Service Manual MEK-8222 C.5

Removing the MASTER Board ........................................................................................... 4.22

Section 5 Socket Pin Assignment ....................................................... 5C.1

Section 6 Adjustment............................................................................ 6C.1

Section 7 Maintenance ......................................................................... 7C.1

C.6 Service Manual MEK-8222

Section 8 Replaceable Parts List......................................................... 8C.1

Section 9 Demonstration Guide .......................................................... 8C.1

Service Manual MEK-8222 C.7

Check with MEK-5D Hematology Control ................................................................. 9.27

C.8 Service Manual MEK-8222

This device is intended for use only by qualified medical personnel.

2. When installing or storing the instrument, take the following precautions:

5. To Shutdown After Use

7. The instrument must not be altered or modified in any way.

8. Maintenance and Inspection:

Service Manual MEK-8222 i

In the USA and Canada other warranty policies may apply.

RESPONSIBILITIES - PROFESSIONAL USERS

ii Service Manual MEK-8222

3. Effect of direct or indirect electrostatic discharge:

Service Manual MEK-8222 iii

iv Service Manual MEK-8222

Warnings, Cautions and Notes

Service Manual MEK-8222 v

Symbol Description Symbol Description

Hand positions for carrying the

Manual RS-232C socket

Clean 2 ZK-820V bar code reader socket

Eject USB socket

Start IVD IVD conformity

Do not touch the sample rack AC power off

Outlet Alternating current

Attention, consult operator's manual Equipotential terminal

Biohazard Year of manufacture

Service Manual MEK-8222 vii

Introduction ........................................................................................................................ 1.1

Service Manual MEK-8222 1C.1