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    Flotation Bath

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    nurul fatinajihah
    This document discusses several instruments and processes used in tissue sample preparation for histological examination, including a flotation bath, cryostat, microtome, tissue processing, embedding, sectioning, staining, and mounting. Key steps involve fixation, grossing, processing, embedding tissues in paraffin blocks, sectioning blocks with a microtome, mounting sections onto slides, and staining before examination under a microscope.

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    Flotation Bath

    Uploaded by

    nurul fatinajihah
    172 views3 pages
    This document discusses several instruments and processes used in tissue sample preparation for histological examination, including a flotation bath, cryostat, microtome, tissue processing, embedding, sectioning, staining, and mounting. Key steps involve fixation, grossing, processing, embedding tissues in paraffin blocks, sectioning blocks with a microtome, mounting sections onto slides, and staining before examination under a microscope.

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    FLOTATION BATH

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    This document discusses several instruments and processes used in tissue sample preparation for histological examination, including a flotation bath, cryostat, microtome, tissue processing, embedding, sectioning, staining, and mounting. Key steps involve fixation, grossing, processing, embedding tissues in paraffin blocks, sectioning blocks with a microtome, mounting sections onto slides, and staining before examination under a microscope.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    172 views3 pages

    Flotation Bath

    Uploaded by

    nurul fatinajihah
    This document discusses several instruments and processes used in tissue sample preparation for histological examination, including a flotation bath, cryostat, microtome, tissue processing, embedding, sectioning, staining, and mounting. Key steps involve fixation, grossing, processing, embedding tissues in paraffin blocks, sectioning blocks with a microtome, mounting sections onto slides, and staining before examination under a microscope.

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    © All Rights Reserved
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    Download as docx, pdf, or txt
    of 3

    FLOTATION BATH:

    - https://www.labce.com/spg605376_instrumentation_for_microtomy_flotation_bath.aspx
    - http://www.tannerscientific.com/manuals/TN450_manual.pdf
    - https://www.google.com/search?
    q=flotation+bath&espv=2&biw=1366&bih=667&tbm=isch&tbo=u&source=univ&sa=X&ved=
    0ahUKEwioiKyg3PDNAhVJtY8KHVb1BFkQsAQIGw
    - http://www.polysciences.com/skin/frontend/default/polysciences/pdf/waterbath.pdf
    -

    CRYOSTAT:
    - http://www.medicinenet.com/script/main/art.asp?articlekey=12235
    - https://en.wikipedia.org/wiki/Cryostat#Continuous-flow_cryostats

    MICROTOME:
    - https://www.labce.com/spg605374_instrumentation_for_microtomy_rotary_microtome_pa
    r.aspx
    - https://en.wikipedia.org/wiki/Microtome

    TISSUE PROCESSING:
    - http://i-learn.uitm.edu.my/main/default-frame.php ?
    - https://www.google.com/search?
    q=process+of+tissue+processing&espv=2&biw=1366&bih=667&tbm=isch&tbo=u&source=u
    niv&sa=X&ved=0ahUKEwiuhJ-R1vXNAhVJLI8KHTf-BtMQsAQIPQ
    - http://www.leicabiosystems.com/pathologyleaders/an-introduction-to-specimen-
    processing/

    TISSUE EMBEDDING:
    - http://www.open.edu/openlearn/science-maths-technology/science/biology/introduction-
    histology/content-section-2.2
    - http://ilearn.uitm.edu.my/CourseModule/ViewUpload.php?
    case=normal&funcfor=course&funcmod=information&courseid=CSI102&lv=1&refid=992335
    &ccshowid=1119839&fcid=755007&nmfile=L6_Tissue
    %20Embedding.pdf&dlfile=/download/2016625096/L6_Tissue%20Embedding.pdf
    - https://www.google.com/search?
    q=process+of+tissue+processing&espv=2&biw=1366&bih=667&tbm=isch&tbo=u&source=u
    niv&sa=X&ved=0ahUKEwiuhJ-R1vXNAhVJLI8KHTf-
    BtMQsAQIPQ#tbm=isch&q=tissue+embedding
    -
    Chapter Content Page
    TITLE PAGE
    ABSTRACT
    TABLE OF CONTENTS
    LIST OF TABLES
    LIST OF FIGURES
    1.0 INTRODUCTION
    1.1 Overview
    1.2 Objective
    1.2.1 General Objective
    1.2.2 Specific Objective
    2.0 MATERIALS AND METHODS
    2.1 Study Venue
    2.2 Materials and Equipments
    2.1.1 Materials
    2.2.2 Equipment
    2.3 Calculation for reagent preparation
    2.4 Tissue fixation procedure
    2.5 Tissue grossing procedure
    2.6 Tissue processing procedure
    2.7 Tissue embedding procedure
    2.8 Tissue sectioning procedure
    2.9 Tissue fishing procedure
    2.10 Tissue staining procedure
    2.11 Tissue mounting procedure
    2.12 Tissue observation procedure
    3.0 RESULTS
    3.1 Sample collection
    3.2 The tissue section slide produced
    3.3 The observation of tissue section under microscopic
    4.0 DISCUSSION
    5.0 CONCLUSION AND RECOMMENDATIONS
    REFERENCES
    APPENDIXES

    Table 2.3 Calculation of fixative reagent


    Table 2.6 Tissue processing procedure
    Table 2.10 Tissue staining procedure

    Figure 2.5 Tissue grossing process


    Figure 2.7 Tissue embedding process
    Figure 2.8 Tissue sectioning process
    Figure 2.11 Tissue mounting process
    Figure 2.12 Tissue observation
    Figure 3.1 3.1. Tissue blocks
    1
    3.1. Tissues after grossing
    2
    Figure 3.2 3.2. Tissue sections after sectioning process
    1
    3.2. Tissue sections after staining process
    2
    Figure 3.3 Observation under compound microscopic

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