CH-2.21 Analyte Reference Interval Instrumentation Methodology Chart

CH-2.
21 Analyte Reference Interval Instrumentation Methodology Chart
Location: Clinical Biochemistry and Hematology\Clinical Biochemistry\02 Reference Manual\Analyte Reference

Document Type: Quick Reference
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Revision Number: 49.01 Last Approved: 2/1/2018 11:37:43 AM
CH-2.21 Analyte Reference Interval Instrumentation Methodology Chart
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Analytes with Critical Values are indicated with red font – refer to CH-2.11 Clinical Biochemistry Critical Values
Site Designations:
FMC Foothills Medical Centre CRL BMSH Banff Mineral Spring Hospital
RRL Rapid ACH Alberta Children’s Hospital CLH Claresholm General Hospital
Response PLC Peter Lougheed Centre CMGH Canmore General Hospital
Lab RGH Rockyview Hospital DDHS Didsbury Health Centre
SHC South Health Campus HRH High River Hospital
HCTL Health ACHC Airdrie Community Health Centre OGH Oilfields General Hospital
Centre CCHC Cochrane Community Health Centre SDHS Strathmore District Health Services
Testing SCHC South Calgary Health Centre VCH Vulcan Health Centre
Labs SMCHC Sheldon M. Chumir Health Centre OHWC Okotoks Health and Wellness Centre
Diagnostic and DSC DSC General Chemistry
Scientific Centre DSC Instrumentation
DSC Urinalysis
Urinalysis Cobas 6000 PLC RGH SHC c501 and e601
ANA TOX Analytical Toxicology ACH c501 only
IMMUNO Cobas 8000 DSC c701, e602 and ISE
Immunospecialchemistry
CHEM FMC c702, e602 and ISE
Cobas c513 DSC c513 (A1C testing)
Cobas c701-c502 DSC c701, c502 and ISE
Cobas e601 Immuno Chem e601 only
Page 1 of 48
CH-2.21 Analyte Reference Interval Instrumentation Methodology Chart Rev 49.01
DOCUMENTATION GUIDELINES
When providing information on reports, documents and the GTS, age and reference intervals should be provided in the following format, when PathNet reporting
does not require alternate formats:
Age  At the lowest end of the age scale use “Less than or equal to XX days/months/years”; NOT “0-XX days”
e.g. Less than or equal to 30 days; NOT 0-30 days
 At the highest end of the age scale use “XX to 150y”
Reference  Greater than or > ; NOT greater than or equal to (≥)
Interval  Less than or < ; NOT less than or equal to (≤)
Analyte Site Instrumentation Method Reference Interval Units Notes

Enzymatic UV assay using
RRL Cobas 6000
G6PDH.
Colorimetric enzymatic assay
FMC Cobas 8000
utilizing arylacylamidase
SMCHC
Acetaminophen CCHC Therapeutic Range: 70 - 130 µmol/L
BMSH CLH
CMGH Vitros Colorimetric slide method.
DDHS HRH
OGH SDHS
VCH
Agilent 7890B Gas
Acetone FMC Gas Chromotography <6 mmol/L
Chromatograph analyzer
2.0 - 11.5
Adrenocorticotropic IMMUNO
Siemens Immulite 2000 Chemiluminescent assay Females on oral contraceptives may have pmol/L
Hormone (ACTH) CHEM
values below normal reference value
DSC FMC Cobas 8000 UV assay without pyridoxal Alternate Name:
Age Male Female
RRL Cobas 6000 phosphate activation Alanine
Alanine
Transferase
Aminotransaminase HCTL CRL Vitros Multiple-point rate slide assay < 18 y 1-35 1-35 U/L
(ALT) - Blood
**ACH **VHF patients
Piccolo Express Colorimetric assay 18-150 y 1-60 1-40
**SHC only
Page 2 of 48

DSC FMC Cobas 8000 The Roche/Hitachi Albumin/BCP
is a colorimetric assay utilizing
RRL Cobas 6000 Age Interval
bromcresol purple
HCTL
Albumin - Blood < 365 d 30-50 g/L
BMSH
CMGH Vitros Colorimetric assay
1-150 y 33-48
DDHS HRH
SDHS VCH
Albumin – CSF DSC Cobas c701-c502 Immunoturbidmetric 0.10 – 0.30 g/L
DSC FMC Cobas 8000 The Roche/Hitachi Albumin/BCP
Albumin - Fluid is a colorimetric assay utilizing No Reference Interval g/L
RRL Cobas 6000 bromcresol purple
Albumin No Reference mg/L
Creatinine Interval mmol/L
<1m < 17.50
Albumin - Urine DSC Cobas c701-c502 Immunoturbidimetric assay Albumin/ Creatinine
1m-2y < 4.00 mg/mmol
Ratio
>2y < 3.00
Albumin Excretion
< 20 µg/min
Rate
Alcohol Refer to Ethanol
Upright at least 30 minutes, with normal salt
IMMUNO intake: 70-1090 pmol/L
Aldosterone - Blood Liaison XL Chemiluminescence pmol/L
CHEM Supine at least 30 minutes, with normal salt
intake: 50-645 pmol/L
An aldosterone (pmol/L)/renin concentration
(mIU/L) ratio >60 may be indicative of
primary aldosteronism if drawn in the
Aldosterone/ Renin IMMUNO
Calculation Calculation absence of confounding drugs. Clinical no units
Ratio CHEM
correlation or further investigation may be
required for confirmation of this
interpretation.
IMMUNO Normal diet nmol/
Aldosterone - Urine Liaison XL Chemiluminescence
CHEM 3 - 78 24hrs
Page 3 of 48

Age Male Female
DSC FMC Cobas 8000 <6y 40-390 40-390
Colorimetric assay. 6-10 y 45-450 45-450
RRL Cobas 6000 11-12 y 55-480 60-480
Alkaline Phosphatase 13-14 y 55-540 45-300 **VHF patients
U/L
(ALP) 15-16 y 50-420 30-160 only
HCTL CRL Vitros Multiple-point rate slide assay 17-18 y 40-200 30-120
19-20 y 30-150 30-115
**ACH 21-49 y 30-130 30-115
Piccolo Express Colorimetric assay
**SHC 50-150 y 30-145 30-145
IMMUNO
Allergy Testing, IgE Unicap Immunoassay test Negative < 0.35 kU/L
CHEM
Alpha-1 Antitrypsin -
DSC Cobas c701-c502 Immunoturbidimetric assay 0.90 - 2.00 g/L
Blood
Alpha-1 Fetoprotein - IMMUNO Chemiluminescent Microparticle
Architect No Reference Interval µg/L
Fluid CHEM Immunoassay technology (CMIA)
Alpha-1 Fetoprotein, IMMUNO Chemiluminescent Microparticle
Architect 0.0 - 10.0 µg/L
Non-pregnant - Blood CHEM Immunoassay technology (CMIA)
Alpha-1 IMMUNO
Optilite Turbidimetric < 12.0 mg/L
Microglobulin - Urine CHEM
Kinetic Interaction of Pre-dose Post-dose
Amikacin ACH Cobas 6000 Microparticles in Solution (KIMS) mg/L
Immunoassay 1.0 - 4.0 20.0 - 25.0
See also
Amiodarone ANA TOX Agilent LC 1100 Liquid Chromatography with DAD 1.5-3.9 µmol/L Desethylamiodar
one
FMC Cobas 8000 Enzymatic kinetic assay utilizing
Ammonia - Blood 12 - 47 µmol/L
RRL Cobas 6000 glutamate dehydrogenase (GLDH).
Amphetamine Screen -
Refer to CH-2.22 Drugs of Abuse Tests
Urine
DSC Cobas 8000 Enzymatic colorimetric assay
Amylase - Blood ACH Cobas 6000 Enzymatic colorimetric assay 30 - 110 U/L
**SHC only
DSC Cobas 8000
Amylase - Fluid Enzymatic colorimetric assay No Reference Interval U/L
ACH Cobas 6000
Page 4 of 48

The formation of p-nitrophenol
directly proportional to a-amylase
Amylase - Urine DSC Cobas c701-c502 0 - 460 U/L
activity measured by absorbance at
409 nm.
IMMUNO Age Male Female
Androstenedione Siemens Immulite 2000 Chemiluminescent assay nmol/L
CHEM 16-150 y 2.1-10.8 1.0-11.5
DSC FMC
Refer to CH-2.42
Anion Gap RRL HCTL Calculation Anion Gap = Na – (CL + CO2) 4-16 mmol/L
Anion Gap
CRL
Refer to Celiac
Anti-Endomysial IMMUNO Screen Reflexive
Manual Indirect Immunoflorescence Slide Negative
Antibodies (AENDAB) CHEM assay for TTG-
IgA > 20kU/L
Male Female
Male and Female
Age Value Age Value < 16 y – Pediatric
<16 y See Note <16 y See Note Reference
No Ref No Ref Intervals are not
16-20 y 16-19 y
Interval Interval available and
20-24 y 11.8-67.8 results should be
Anti-Mullerian IMMUNO interpreted in
Cobas e601 Chemiluminescence assay 25-29 y 8.4-65.4 pmol/L
Hormone CHEM context with the
10.2 – 30-34 y 4.8-53.9 clinical
21-70 y
82.8 35-39 y 5.5-37.4 presentation.
40-44 y 0.7-21.1 Please contact
Pediatric
45-50 y 0.3-14.7 Endocrinology
No Ref No Ref for consultation
71-150 y 51-150 y
Interval Interval
Anti-Nuclear
Refer to Nuclear Antibodies - Blood
Antibody (ANA)
Anti-Streptolysin O
DSC Cobas c701-c502 Immunoturbidimetric assay 0 - 200 IU/mL
Titre (ASOT)
Anti-Thyroglobulin
Refer to Thyroglobulin Antibodies
Antibody (Anti-TG)
Anti-Thyroid
Peroxidase, Anti-
Thyroid Antibodies,
Refer to Thyroid Peroxidase Antibody
Thyroid Antibodies,
Anti-Microsomal Abs
or Anti-TPO
Page 5 of 48

Apolipoprotein IMMUNO Male Female
Optilite Turbidimetric g/L
(A-1) (APOA) CHEM 1.10 - 1.70 1.20 - 1.90
< 18 y < 0.90
Acceptable limit relative to dyslipidemia

and atherosclerosis risk is
< 0.9 g/L
18 - 150 y < 1.20
If result is ≥ 1.2 g/L :

Treatment advised if FRS is Intermediate or
Apolipoprotein (B) IMMUNO
Optilite Turbidimetric High (Refer to Non – HDL Cholesterol for g/L
(APOB) CHEM
FRS ranges)
Treatment target for
ApoB < 0.8 g/L
(2016 CCS Guideline, Can J Cardiol 2016)
If result is < 1.2 g/L:

Treatment target for
ApoB < 0.8 g/L
(2016 CCS Guideline, Can J Cardiol 2016)
DSC FMC Cobas 8000 Alternate Name:
UV assay Age Male Female Aspartate
RRL Cobas 6000
Aspartate <1 y 10-65 10-65 Transaminase
Aminotransferase DDHS HRH 1-3 y 10-55 10-55 U/L
Vitros Multiple - point rate slide assay
(AST), Blood SDHS 4-10 y 10-45 10-45
**ACH 11-150 y 8-40 8-32 **VHF patients
**SHC only
B
Barbiturate Screen -
Urine
Page 6 of 48

FMC RRL
This analyte is
BMSH
part of a panel
CMGH
GEM 4000 test
DDHS
HRH
*Established by
SDHS
AHS Calgary
Zone
Base Excess (BE) CLH GEM 3500 HCO3 – 24.8 + 16.2 (pH – 7.40) * - 5 to + 1 mmol/L Respiratory
Services
**VHF patients
**ACH
only
**SHC
i-STAT System
i-STAT CG4+
OGH Cartridge
VCH
Bence Jones Protein -
Go to: Electrophoresis, Protein - Urine
Urine
Benzodiazepine
Screen - Urine
Beta-2 Glycoprotein IMMUNO
BioPlex Multiplex Flow Assay Negative No units
Antibody CHEM
IMMUNO Chemiluminescent
Beta-2 Microglobulin Siemens Immulite 2000 1.00 - 2.60 mg/L
CHEM immunometric assay
Liquid Chromatography with
Beta-Carotene ANA TOX Agilent LC 1100 0.19 - 1.58 µmol/L
DAD
FMC RRL
HCTL The Quidel QuickVue One-Step
Beta HCG, CRL hCG Combo kit or Quidel Quick
Manual No Reference Interval
Qualitative - Urine Vue One-Step hCG Urine kit is a
DSC qualitative colorimetric method.
Urinalysis
Page 7 of 48

DSC FMC Cobas 8000
Electrochemiluminescence
RGH PLC immunoassay (ECLIA)
Cobas 6000 STATS from
SHC
Enzyme Immunoassay Sandwich ACH go to
BMSH mini VIDAS FMC
Beta HCG, method Males and non-pregnant females:
CMGH Two-site immunoenzymatic IU/L
Quantitative - Blood Access 0-5
HRH (sandwich) assay
**VHF patients
**ACH Two-site enzyme –linked only
i-STAT System
**SHC immunosorbant (ELISA) method i-STAT BhCG
Cartridge
FMC RRL
BMSH *Established by
CMGH Log HCO3 = pH + log pCO2 – AHS Calgary
GEM 4000 Zone
DDHS 7.608
HRH *20-24 Respiratory
SDHS Services
**ACH Log HCO3 = pH + log pCO2 –
i-STAT System This analyte is
**SHC 7.608
Bicarbonate (HCO3) mmol/L part of a panel
test
CLH GEM 3500 Derived analyte
Arterial 18 - 23 **VHF patients
Venous 22 - 28 only
Capillary 20 - 24
OGH Log HCO3 = pH + log pCO2 – i-STAT CG4+
i-STAT System
VCH 7.608 Cartridge
<1 m: 70 µmol/L
1 – 3 m: < 41 µmol/L
Bile Acids DSC Cobas c701-c502 Enzymatic Colorimetric µmol/L
3 – 12 m: < 30 µmol/L
>12 m: < 8.2 µmol/L
DSC FMC Cobas 8000 Colorimetric assay utilizing a
RRL Cobas 6000 diazonium ion with blank
Age Value
HCTL Alternate
Bilirubin, Direct BMSH Name:
< 15 d (Neonatal) 0 – 20 µmol/L
(Conjugated) CMGH Conjugated
Vitros Colorimetric slide assay Bilirubin
DDHS 15 d - 150 y 0-7
HRH OGH
SDHS
Page 8 of 48

DSC FMC Cobas 8000 Age Value
<1d 0-100 Neonatal
RRL Cobas 6000 Colorimetric assay utilizing a Neonatal 1-2 d 0-150 Bilirubins done
diazonium ion with blank. 3-14 d 0-220 only at DSC
Bilirubin, Total 15-365 d 0-23 µmol/L FMC RRL
1-4 y 0-10 CMGH DDHS
HCTL
Vitros Colorimetric slide assay 5-9 y 0-14 HRH OGH
CRL
10-14 y 0-19 SDHS
15-150 y 0-24
Age Value
<1d 5-108
Bilirubin, Total 1-2 d 5-159
3-14 d 5-231
Piccolo Xpress **ACH **VHF patients
Piccolo Express Colorimetric assay 15-365 d 5-29 µmol/L
Analyzer **SHC only
1-4 y 5-15
VHF patients only 5-9 y 5-19
10-14 y 5-25
15-150 y 5-30
Bilirubin, Total - DSC FMC Cobas 8000 Colorimetric assay utilizing a
No Reference Interval µmol/L
Fluid RRL Cobas 6000 diazonium ion with blank.
Blood - Urine Go to: Urinalysis
This is a panel test which may include the following, dependent on site and instrumentation: pH, pCO2, pO2, Bicarbonate (HCO3), Base Excess (BE),
Blood Gases Total Hemoglobin, O2 Saturation (Oxyhemoglobin), Carboxyhemoglobin, Methemoglobin, Hematocrit, Sodium, Potassium, Chloride, Ionized Calcium,
Glucose and Lactate. Refer to individual analytes.
C
IMMUNO
CA 125 - Blood Cobas e601 Chemiluminescence assay Female: < 36 KU/L
CHEM
IMMUNO
CA 125 - Fluid Cobas e601 Chemiluminescence assay No Reference Interval KU/L
CHEM
IMMUNO Chemiluminescent Microparticle
CA 15-3 Architect < 31.2 kU/L
CHEM Immunoassay
IMMUNO
CA 19-9 - Blood Cobas e601 Chemiluminescence assay < 36 KU/L
CHEM
IMMUNO
CA 19-9 - Fluid Cobas e601 Chemiluminescence assay No Reference Interval KU/L
CHEM
Page 9 of 48

DSC FMC Cobas 8000 Colorimetric assay with endpoint
determination and sample blank
RRL Cobas 6000 utilizing NM-BAPTA Age Value
SMCHC
BMSH CLH 1-10 d 1.90-2.60
Calcium - Blood CMGH mmol/L
Vitros Colorimetric slide assay
DDHS HRH 11-365 d 2.25-2.75
OGH SDHS
VCH 1-150 y 2.10-2.55
**SHC only
DSC FMC Cobas 8000 Colorimetric assay with endpoint
Calcium - Fluid determination and sample blank No Reference Interval mmol/L
RRL Cobas 6000 utilizing NM-BAPTA
*Established by
CLS Chemistry
Age *Value Division.
FMC Potentiometric sensor which is Also done by
GEM 4000 < 29 d 0.90-1.30
RRL located in a cartridge Respiratory at
Calcium, Free or 29 d-150 y 1.15-1.35 ACH, FMC,
mmol/L
Ionized PLC, RGH and
SHC
Age Value **VHF patients
**ACH Measured by ion-selective only
i-STAT System < 29 d 0.90-1.30
**SHC electrode (ISE) potentiometry i-STAT Chem
29 d-150 y 1.15-1.35 8+
Colorimetric assay with endpoint
Liquid Random
Calcium - Stool FMC Cobas 8000 determination and sample blank No Reference Interval mmol/L
Stool
utilizing NM-BAPTA.
o-cresolphthalein complex one
mmol/
Calcium - Urine DSC Cobas c701-c502 reaction measured photometrically 2.5 - 7.5
day
at 552 nm.
Refer to CH-
Calcium/Creatinine 55.07 Cobas
DSC Cobas c701-c502 Calculation No Reference Interval
Ratio, Urine c701-c502
Calculations
Renal calculi composition is
Calculus Analysis DSC Nicolet FTIR determined by infrared No Reference Interval
spectroscopy.
Cannabinoid Screen –
Urine (THC)
Page 10 of 48

Kinetic Interaction of
Alternate
Carbamazepine FMC Cobas 8000 Microparticles in Solution (KIMS) Therapeutic Range: 20 - 50 µmol/L
Name : Tegretol
Immunoassay
*Established by
CLS and AHS
FMC Age *Value Calgary Zone
RRL BMSH Respiratory
Carboxyhemoglobin <8y No Reference Interval
CMGH GEM 4000 CO-Oximetry % Services
(COHB)
DDHS HRH
SDHS 8 y – 150 y 0.0 - 3.0 This analyte is
part of a panel
test
Carcinoembryonic IMMUNO Chemiluminescent Microparticle
Architect 0.0 - 5.0 µg/L
Antigen (CEA) - Blood CHEM Immunoassay technology (CMIA)
Carcinoembryonic IMMUNO Chemiluminescent Microparticle
Architect No Reference Interval µg/L
Antigen (CEA) - Fluid CHEM Immunoassay technology (CMIA)
IMMUNO
Cardiolipin Antibody BioPlex Multiplex Flow Assay Negative No units
CHEM
Norepinephrine Epinephrine
Age (nmol/L) (pmol/L)
(Resting)
2 - 10 d 1.00 - 6.97 197 - 2183
10 d - 4 m 2.18 - 12.29 300 - 1092
4 - 12 m 1.59 - 6.62 300 - 2402
Refer to
12 - 24 m 0.40 - 10.69 197 - 3493 CH10-2.10
Catecholamine, Blood ANA TOX Agilent LC 1100 Liquid Chromatography with ECD 2-3y 1.00 - 8.68 98 - 2402 Catecholamines
3 - 18 y 0.50 - 7.38 98 - 2511 - HPLC
Coulochem
18 - 150 y 0.41 - 4.43 55 - 601
Dopamine: 2 d - 150 y is
0 - 200 pmol/L
Clonidine Suppression Test: Normal response - a
maximum fall of plasma norepinephrine to less than
2.96 nmol/L and a decrease of greater than 50% from
basal values.
Page 11 of 48

Male Female
Ceruloplasmin DSC Cobas c701-c502 Immunoturbidimetric assay g/L
0.15- 0.30 0.16 – 0.45
DSC FMC Cobas 8000 Indirect potentiometric assay using ***VHF
RRL Cobas 6000 an ion-selective electrode. patients only
Chloride - Blood HCTL CRL Vitros Direct potentiometric assay 98-111 mmol/L
i-STAT Chem
***ACH
i-STAT System Potentiometric 8+ Cartridge
***SHC
Established by
CLS Chemistry
Chloride – Blood 98 - 111 Division
FMC RRL GEM 4000 Potentiometric mmol/L
(Blood Gases) This analyte is
part of a panel
test
DSC FMC Cobas 8000 Indirect potentiometric assay using
Chloride - Fluid No Reference Interval mmol/L
RRL Cobas 6000 an ion-selective electrode
Indirect potentiometric assay using
Chloride – Stool FMC Cobas 8000 No Reference Interval mmol/L
an ion-selective electrode
DSC Cobas c701-c502 mmol/
Indirect potentiometric assay using 24 hour 170-255
Chloride – Urine FMC Cobas 8000 day
an ion-selective electrode
RRL Cobas 6000 Random No Reference Interval mmol/L
Age Male/Female
< 366 d 2.36 - 5.32
Enzymatic colorimetric assay Acceptable limit relative to
DSC Cobas 8000
(CHOD-PAP) dyslipidemia and
atherosclerosis risk is < 4.40
mmol/L
Cholesterol, Total –
366 d to 2.70 – 5.89 mmol/L
Blood
17 y Acceptable limit relative to
dyslipidemia and
Cholesterol oxidase, esterase,
BMSH Vitros atherosclerosis risk is < 4.40
peroxidise
mmol/L
18 to 150 y Desirable < 5.17

High ≥ 6.21
Enzymatic colorimetric assay
Cholesterol – Fluid DSC Cobas 8000 No Reference Interval mmol/L
(CHOD-PAP)
Cholinesterase Go to: Pseudocholinesterase (Acetycholinesterase is a sendout to DynaLife Dx)
IMMUNO
Chromogranin A Triturus Enzyme Immunoassay < 111 ng/mL
CHEM
Page 12 of 48

Chylomicrons, Visual CH-6.30
– Blood Chylomicron in
DSC Manual Reported as present or absent No Reference Interval None
Chylomicrons, Visual - Serum Plasma
Fluid and Fluids
DSC FMC Cobas 8000
Liquid IFCC is an UV assay
RRL Cobas 6000
BMSH CLH Male Female
CK – Blood
CMGH U/L
(Creatine Kinase)
DDHS HRH Vitros Multiple- point rate 0 – 195 0 – 170
OGH SDHS
VCH
PSC: Order
The CK-MB STAT is an
Troponin T-hs
CK-MB FMC Cobas 8000 Electrochemiluminescence 0.0 – 5.0 µg/L
instead of
immunoassay (ECLIA)
CKMB
Recommended clozapine interval is 1070 to
1835 nmol/L to achieve a therapeutic
response. Significant CNS toxicity may be
Clozapine ANA TOX Agilent LC 1100 Liquid Chromatography with DAD nmol/L
associated with levels >3500 nmol/L for
clozapine and >2000 nmol/L for
desmethylclozapine.
DSC FMC Cobas 8000 CO2-L is an enzyme assay utilizing
RRL Cobas 6000 PEPC Age Value
CO2 Content – Blood < 6 y 18-27
HCTL CRL Vitros Enzymatic endpoint slide assay mmol/L
(Carbon Dioxide)
***ACH 6-150 y 21-31 ***VHF
***SHC patients only
DSC FMC Cobas 6000 CO2-L is an enzyme assay utilizing Done on
CO2 Content – Fluid No Reference Interval mmol/L
RRL Cobas 8000 PEPC dialysates only
Cocaine Metabolite
Screen, Urine
Complement C3 -
DSC Cobas c701-c502 Immunoturbidimetric assay 0.6-1.6 g/L
Blood
Complement C3 -
DSC Cobas c701-c502 Immunoturbidimetric assay No reference interval g/L
Synovial Fluid
Complement C4 DSC Cobas c701-c502 Immunoturbidimetric assay 0.1-0.4 g/L
DSC Cobas 8000 Cortisol AM: 170-500
Electrochemiluminescent
Cortisol - Blood Cortisol PM: 75-285 nmol/L
FMC Cobas 8000 immunoassay
Cortisol: No reference interval
Cortisol - Oncology Refer to Oncology Cortisol
Page 13 of 48

IMMUNO AM 4.7-32.0
Cortisol - Salivary Cobas e601 ECLIA nmol/L
CHEM PM 0.0-3.6
IMMUNO
Cortisol - Urine Cobas e601 ECLIA 24 hour 100.0 – 380.0 nmol/d
CHEM
IMMUNO
C-Peptide Siemens Immulite 2000 Chemiluminescent assay Fasting 0.40-1.40 nmol/L
CHEM
DSC FMC Cobas 8000
C-Reactive Protein Immunoturbidimetric assay
RRL Cobas 6000 0.0-8.0 mg/L
(CRP) (Quantitative)
HRH Vitros Fixed-point Immuno rate
C-Reactive Protein,
Particle enhanced A reference interval for High Sensitivity
High Sensitivity DSC Cobas 8000 mg/L
Immunoturbidimetric assay CRP is not available
(hsCRP)
Creatine Kinase Refer to CK-Creatine Kinase
Age Male & Female
DSC FMC Cobas 8000
Enzymatic colorimetric assay Unknown
utilizing creatininase <2y 10-40 10-40
RRL Cobas 6000
Creatinine - Blood 2-5 y 20-45 20-45 µmol/L
HCTL CRL Vitros 2 point rate assay 6-12 y 20-75 20-75
***ACH 13-14 y 30-95 30-95 ***VHF
Piccolo Express Colorimetric assay 15-150 y 50-120 40-100
***SHC patients only
DSC FMC Cobas 8000 Enzymatic colorimetric assay
Creatinine - Fluid No Reference Interval µmol/L
PLC Cobas 6000 utilizing creatininase
DSC Cobas c701-c502
Creatinine - Urine utilizing creatininase
FMC Cobas 8000 No Reference Interval mmol/L
(Random) Enzymatic colorimetric assay
RRL Cobas 6000 utilizing creatininase
Page 14 of 48

Age Male Female
<3y no range no range
3-8 y 1.20-6.20 1.20-6.20
Creatinine - Urine Enzymatic colorimetric assay 9-12 y 2.70-11.50 2.70-11.50
DSC Cobas c701-c502 mmol/d
(24 hour) utilizing creatininase 13-17 y 4.40-20.30 3.50-14.10
18-50 y 8.80-22.10 6.20-14.10
51-80 y 7.00-18.60 4.40-12.40
81-150 y 5.30-17.70 3.50-11.50
Refer to CH-
55.07 Cobas
Creatinine Clearance DSC Calculation 1.20-2.30 mL/s/1.73 m2
c701-c502
Calculations
Refer to GL-
14.02 Fluid
Crystal
FMC PLC
The identification of crystals Identification –
RGH SHC
Crystals, Synovial Manual-Polarizing within exudative synovial fluid is Specimen or
No Reference Interval
Fluid Microscopy performed using a compensating GL-14.06 Fluid
DSC
polarizing microscope. Crystal
Urinalysis
Identification -
FMC PLC
RGH SHC
CTx (C-Telopeptide – IMMUNO
Cobas e601 Chemiluminescent assay 0-400 ng/L
Beta Cross Laps) CHEM
Cyclic Citrullinated
IMMUNO Chemiluminescent microparticle
Peptide Antibody Architect < 5.0 U/mL
CHEM Immunoassay (CMIA)
(Anti-CCP)
Results interpreted by clinician.
Interpretation is dependent upon various
Agilent LC with ABsciex
Cyclosporin Ana Tox Tandem Mass Spectrometry factors such as transplant type, time post µg/L
MS
transplant, and concomitant
immunosuppressive therapy
D
Depakene Go to: Valproate
Refer to CH10-2.01
Desethyl-amiodarone Ana Tox Agilent LC 1100 1.3-3.5 µmol/L Amiodarone -
DAD
HPLC DAD
DHEA-S IMMUNO Siemens Immulite 2000 Chemiluminescent assay Age Male Female µmol/L
Page 15 of 48

(Dehydroepiandroste CHEM 1d-10y 0.3-6.0 0.3-6.0
rone Sulphate) 11-54y 2.2-13.0 1.5-13.0
55-150y 0.6-10.0 0.3-6.0
Normal individuals: > 0.74
To determine the dibucaine
Dibucaine Number Heterozygous Refer to CH-55.07
number (DN), cholinesterase 0.57-0.72 No
(See also DSC Calculation individuals: Cobas c701-c502
activity is measured with and Units
Pseudocholinesterase) Homozygous Calculations
without dibucaine. < 0.51
individuals:
FMC Cobas 8000 Kinetic Interaction of Therapeutic range: 1.0-2.6
PLC RGH Microparticles in Solution
Cobas 6000
Digoxin SHC (KIMS) Immunoassay nmol/L
BMSH Evidence-based therapeutic range for
Vitros Multiple-point immuno-rate assay CHF: 0.6-1.2
HRH SDHS
Dilantin / Dilantin,
Go to: Phenytoin / Phenytoin, Free
Free
0–9
IMMUNO
DNA Antibodies BioPlex Multiplex Flow Assay Negative: <5 kIU/L
CHEM
Indeterminate: 5 – 9
Positive: >9
Drugs Of Abuse Screen, Urine (UDS –Acute Care)
Drugs Of Abuse Screen, Urine (UDSR – Routine) Refer to CH-2.22 Drugs of Abuse Tests
Drug Screen, Comprehensive(FDSU)
E
Albumin 33 - 48
Alpha 1 Globulin 2.2 - 4.0
Electrophoresis, Alpha 2 Globulin 5 -10
DSC Capillyrs/Hydrasys Electrophoresis g/L
Protein - Serum Beta 1 Globulin 3-6
Beta 2 Globulin 2-5
Gamma Globulin 5 - 16
Electrophoresis,
DSC Capillyrs/Hydrasys Electrophoresis No Reference Interval
Protein - Urine
IMMUNO
Erythropoietin Siemens Immulite 2000 Chemiluminescent assay 4-20 U/L
CHEM
Page 16 of 48

Female
< 11 y (pre-puberty) 0-130
Follicular phase 90-700
Luteal phase 150-950
Competition principle using Mid-cycle phase 250-1500
Estradiol DSC Cobas 8000 pmol/L
chemiluminescent technology 61-150 y (post- 0-150
menopause)
Male
< 11 y 0-130
11-150 y 0-160
FMC Cobas 8000 Enzymatic method with alcohol
RRL Cobas 6000 dehydrogenase
SMCHC
CCHC
BMSH
Ethanol - Blood CMGH No Reference Interval mmol/L
CLH Vitros Colorimetric slide assay
DDHS
HRH
SDHS
VCH
Ethanol - Urine Refer to CH-2.22 Drugs of Abuse Tests
Agilent 7890B Gas
Ethylene Glycol FMC Chromatograph Gas chromatography No Reference Interval mmol/L
analyzer
Includes the
following:
Centromere B,
Chromatin,
Jo-1,
Ribosomal P,
Extractable Nuclear
IMMUNO RNP 68,
Antigen Antibodies - BioPlex Multiplex Flow Assay Negative
CHEM RNP A,
Blood
Ro-52/TRIM21,
Scl-70,
SS-A/Ro-60, SS-
B/La,
Sm, SmRNP,
dsDNA
Page 17 of 48
F
Refer to GL-15.02
Fat Globules, DSC Fat Globules and
Manual < 6 fat globules/hpf
Random – Stool Urinalysis Meat Fibers -
Stool
Fecal Fat Output Fecal Fat
A weighed sample is manually mmol/
<7y 0.0-6.9 Excretion Range
extracted, evaporated, dissolved day
7-150 y 0.0-20.9 only calculated for
Fecal Fat – 72 Hour ACH Manual and titrated to obtain a
patients assessed
measurement of the total fatty Fat Excretion Range
% for fat intake at
acids present. All ages 0.0-9.9 %
ACH
IMMUNO
Fecal Elastase Manual Enzyme immunoassay > 199 µg/g
CHEM
As of November
18, 2013 this test
replaced Occult
Blood – Stool for
Fecal
DSC community
Immunochemical OC-Sensor Diana Immunoassay Negative
Urinalysis patients
Test (FIT)
For screening
asymptomatic 50-
74 year olds.
Age Male Female
Particle Enhanced 1d-2 y 6-110 6-110
Ferritin DSC Cobas 8000 µg/L
Immunoturbidmetric Assay 3-12 y 10-110 10-110
13-150 y 30-400 13-375
First Trimester IMMUNO Perkin Elmer Auto Solid phase 2 site Includes PAPP-A
No Reference Interval
Screen CHEM Delfia fluoroimmunometric assays. And Free BHCG
IMMUNO
Folate - Serum Cobas e601 Chemiluminescence assay >12.0 nmol/L
CHEM
Page 18 of 48

Female
1d – 1y 0-7
2-5y 0-4
6-10y 0-3
Follicular 2-10
Two-site sandwich immunoassay Luteal 1-9
Follicle Stimulating
DSC Cobas 8000 using chemiluminescent IU/L
Hormone (FSH) Mid-Cycle Peak 3-33
technology.
Post-Menopause 23-116
Male
1d – 10y 0-2
11-12y 0-8
13-150y 1-18
This is a calculation done by Male Female Refer to CH-37.07
Free Androgen Index IMMUNO
Calculation using the testosterone and the No Units Immulite
(FAI) CHEM > 29.9 < 5.1
SHBG results. Calculations
Free Prostatic
Specific Antigen Go to: PSA, Fractionated/Free
(FPSA)
Fructosamine DSC Cobas c701-c502 Colorimetric method. 200-285 µmol/L
G
DSC FMC Cobas 8000
RRL Cobas 6000
HCTL
BMSH Male Female
Gamma Glutamyl-
CMGH Vitros Multiple point rate assay U/L
transferase (GGT)
DDHS 11-63 8-35
HRH SDHS
***ACH ***VHF patients
***SHC only
IMMUNO
Gastrin Siemens Immulite 2000 Chemiluminescent assay Fasting >10 hours: < 100 ng/L
CHEM
Page 19 of 48

Therapeutic Target for Multiple-
Fluorescence Polarization Daily Dosing
FMC Cobas 8000 Pre-dose 0.0 - 1.9
immunoassay.
Post-dose 5.0 - 10.0
Gentamicin Therapeutic Target mg/L
for Extended Interval Dosing
Pre-dose 0.0-1.0
RRL Cobas 6000 Enzymatic Immunoassay
Contraindicated for
Post-dose adults with normal
renal function
Glomerular
IMMUNO
Basement Membrane BioPlex Multiplex Flow Assay < 1 AI (Antibody Index)
CHEM
Antibody (GBM)
Refer to CH-2.45
Glomerular DSC FMC Calculation using Creatinine and
Estimated
Filtration Rate RRL HCTL Calculation age of patient. Done on all >59 mL/min/1.73 sq.m
Glomerular
(GFR) CRL patients > 17 y
Filtration Rate
DSC FMC Cobas 8000 Fasting
Hexokinase-UV assay < 30 d 2.5 – 5.5
RRL Cobas 6000 30 d – 150 y 3.3 – 6.0
Glucose - Blood mmol/L
HCTL CRL Vitros Glucose Oxidase - Colorimetric Random
***ACH Modified Hexokinase- < 30 d 2.5 – 11.0 ***VHF patients
Piccolo Express
***SHC Colorimetric 30 d – 150y 3.3 – 11.0 only
Age
Value Established by
CLS Chemistry
Glucose - Blood < 29 d
FMC RRL GEM 4000 Amperometric sensors 2.4 - 5.6 (fasting) mmol/L Division
(Blood Gases)
This analyte is
29 d - 150 y
3.9 – 6.1 (fasting) part of a panel test
DSC FMC Cobas 8000 Age Values
Hexokinase-UV assay
Glucose - CSF RRL Cobas 6000 < 31 d 1.4 - 4.3 mmol/L
BMSH Vitros Glucose Oxidase - Colorimetric 31 d – 150 y 2.2 - 3.9
DSC FMC Cobas 8000
Glucose - Fluid Hexokinase-UV assay No Reference Interval mmol/L
RRL Cobas 6000
Glucose - Urine
ACH Cobas 6000 Hexokinase-UV assay No Reference Interval mmol/L
Quantitative
Glucose - Urine
Go to: Urinalysis
Qualitative
Page 20 of 48

< 7.8 mmol/L at 1 hour after 50g
glucose load
DSC FMC Cobas 8000
Test should be performed between 24-
Hexokinase-UV assay 28 weeks gestation.
Glucose - Gestational
RRL Cobas 6000 A glucose tolerance test is warranted if mmol/L
Screen for Diabetes
the 1 hour glucose value is 7.8-11.0
mmol/L
CRL Vitros Glucose Oxidase - Colourimetric Gestational diabetes mellitus is

diagnosed if the 1 hour glucose value is
greater than or equal to 11.1 mmol/L
Page 21 of 48

DSC FMC Cobas 8000 Hexokinase-UV assay NOTES:
RRL Cobas 6000 Hexokinase-UV assay GTTNP
If fasting glucose value is over 7.8
mmol/L when tested by glucose
meter the GTTNP will be cancelled.
mmol/L
GTTP
CRL Vitros Glucose Oxidase - Colourimetric If fasting glucose is over 5.9
mmol/L when tested by glucose
meter or over 5.2 mmol/L when
tested by chemistry analyzer the
GTTP will be cancelled.
REFERENCE INTERVALS:
Fasting Specimen (GTTNP):

Less than 30 d: 2.5-5.5
30 d to 150 y: 3.3-6.0
Glucose Tolerance
(GTTNP or GTTP) Non-Pregnant Adults/Children <17 (GTTNP):
Diagnosis of Diabetes Mellitus: Fasting plasma glucose > 6.9 mmol/L or 2 h post administration of the 75g glucose drink > 11.0 mmol/L in the
presence of symptomatic hyperglycemia, or observed on two occasions without symptoms
Diagnosis of impaired fasting glucose (Prediabetes): Fasting glucose 6.1 to 6.9 mmol/L AND plasma glucose 2 hours post administration of the
75g glucose drink < 7.8 mmol/L
Diagnosis of impaired glucose tolerance (Prediabetes): Fasting plasma glucose < 6.1 mmol/L AND plasma glucose 2 hours post administration of
the 75 g glucose drink between 7.8 and 11.0 mmol/L
Diagnosis of impaired fasting glucose and impaired glucose tolerance (Prediabetes): Fasting plasma glucose between 6.1 and 6.9 mmol/L AND
plasma glucose 2 hours post administration of the 75g glucose drink between 7.8 and 11.0 mmol/L
Pregnant (GTTP):
Note: the 75g Oral Glucose Tolerance Test for Gestational Diabetes Mellitus (GDM) should be performed if the Gestational Diabetes Screen result is
between 7.8 and 11.0 mmol/L
Diagnosis of Gestational Diabetes Mellitus:

GDM is diagnosed if one or more of the following values are elevated during the 75g Oral Glucose Tolerance Test:
Fasting glucose > 5.2 mmol/L
Plasma glucose 1 hour post administration of glucose drink > 10.5 mmol/L
Plasma glucose 2 hours post administration of glucose drink > 8.9 mmol/L
IMMUNO Male Female
Growth Hormone Siemens Immulite 2000 Chemiluminescent assay µg/L
CHEM 0-3 0-8
Page 22 of 48
H
Instrumentation and
Analyte Site* Method Reference Interval Units Notes
Additional Info
Haptoglobin DSC Cobas c701-c502 Immunoturbidimetric assay 0.3-2.0 g/L
The Roche/Hitachi HDL-C
DSC Cobas 8000 Plus is an enzymatic
HDL Cholesterol colorimetric assay No Reference Interval mmol/L
BMSH Vitros Colorimetric assay
FMC RRL
Hematocrit CMGH Refer to: This analyte is
GEM 4000 Derived analyte
(Blood Gases) DDHS HRH HE-1.21 CBC Reference Intervals part of a panel test
SDHS
FMC RRL Refer to:

Hemoglobin, Total CMGH HE-1.11 Hematology Critical Values This analyte is
GEM 4000 CO-Oximetry g/L
(Blood Gases) DDHS HRH or part of a panel test
SDHS HE-1.21 CBC Reference Intervals
Hemoglobin A1c DSC Cobas c513 Colorimetric method. < 6.5 % %
Hepatitis A Antibody,
IgG Negative
(Anti-HAV IgG)
Hepatitis A Antibody,
IgM Negative
(Anti- HAV IgM)
Hepatitis B Core
Antibody Negative
(Anti-HBc)
Hepatitis B Core
Antibody, IgM Negative
IMMUNO Microparticle Enzyme
(Anti-HBc IgM) Architect
CHEM Immunoassay (MEIA)
Hepatitis Be Antigen
Negative
(HBeAg)
Hepatitis Be Antibody
Negative
(Anti-HBe)
Antibody to Hepatitis B Surface
Hepatitis B Surface
Antigen levels greater than or equal to
Antibody IU/L
10 IU/L are generally considered
(Anti-HBs)
protective
Hepatitis B Surface
Antigen Negative
(HBsAg)
Page 23 of 48
Instrumentation and
Analyte Site* Method Reference Interval Units Notes
Additional Info
24 Hour
µmol/d
All Ages 10-40 Refer to CH10-
5 HIAA (5-
Agilent LC 1100 with 1.20 5-Hydroxy
Hydroxyindoleacetic ANA TOX Tandem Mass Spectrometry Random
MS 6410 µmol/ indoleacetic Acid
Acid) <6y < 13
mmol - 6410 6460
6 y to 150 y < 10
Chemiluminescent
IMMUNO
HIV Serology, CLS Architect Microparticle Immunoassay Negative no units
CHEM
technology (CMIA)
Refer to GL-16.02
HIV Serology by FMC PLC 24 INSTI™ - HIV-1 Antibody
Manual Non-Reactive HIV Serology by
Rapid Assay RGH SHC Test Kit
Rapid Assay
Chemiluminescent Age Male Female
IMMUNO
Homocysteine - Blood Architect Microparticle Immunoassay 16-49 y 4.9-13.7 4.1-9.9 µmol/L
CHEM
technology (CMIA) 50-150 y 4.9-13.7 3.8-12.8
A measured aliquot of breath
carried through the HMS
column by room air acting as
the carrier gas separates
Hydrogen Breath Test ANA TOX Quintron No Reference Interval
hydrogen (H2) and methane
(CH4), which appear
sequentially at the end of the
column.
I
Immunofixation
Electrophoresis (IFE) DSC Capillarsys/Hydrsys Electrophoresis No Reference Interval
- Blood/Urine
Page 24 of 48

Age Male/Female
1 d-2 m 0.00-1.20
3 m-5 m 0.05-1.20
6-12 m 0.10-1.20
1y 0.20-1.60
Immunoglobulin A -
DSC Cobas c701-c502 Immunoturbidimetric assay 2y 0.30-2.00 g/L
Blood
3-5 y 0.35-2.40
6-7 y 0.40-2.80
8-10 y 0.45-3.20
11-15 y 0.50-3.80
16-150 y 0.60-4.20
Age Mean +2SD
<1y 8.0 117.0
1-4y 9.0 313.0
IMMUNO 5 - 10 y 18.0 555.0
Immunoglobulin E Cobas e601 Chemiluminescence assay kU/L
CHEM
11 - 15 y 26.0 481.0
Adult: approximately 95% of adult values
fall between 0.0 and 160.0 kU/L with a
geometric mean value of 17.0 kU/L
Free Kappa Light Chain: 3.30-19.40
Immunoglobulin IMMUNO Free Lambda Light Chain: 5.71-26.30
Optilite Turbidimetric mg/L
Free Light Chains CHEM Free Kappa/Free Lambda Ratio:
0.26-1.65
Age Male/Female
1 d-2 m 2.60 - 14.00
3-12 m 2.80 - 16.00
1-2 y 4.00 - 16.00
Immunoglobulin G -
DSC Cobas c701-c502 Immunoturbidimetric assay 3-5 y 5.40 - 16.00 g/L
Blood
6-7 y 5.80 - 16.00
8-10 y 6.20 - 17.00
11-15 y 6.40 - 17.00
16-150 y 6.80 - 18.00
Immunoglobulin G -
DSC Cobas c701-c502 Immunoturbidimetric assay No Reference Interval g/L
CSF
Page 25 of 48

Subclass Male/Female
IgG Subclasses, IgG1 3.82 – 9.29
IMMUNO
Subgroups or Optilite Turbidimetric IgG2 2.42 – 7.00 g/L
CHEM
Subtypes IgG3 0.22 – 1.76
IgG4 0.04 – 0.86
Age Male/Female
<5m 0.14 – 1.40
5m–3y 0.20 – 1.60
Immunoglobulin M DSC Cobas c701-c502 Immunoturbidimetric assay 4y–6y 0.20 – 2.10 g/L
7 y – 11 y 0.30 – 2.10
12 y – 19 y 0.30 – 2.40
20 y – 150y 0.40 – 3.00
Chemiluminescent
IMMUNO pmol/
Insulin Architect Microparticle Immunoassay Fasting < 120
CHEM L
technology (CMIA)
Insulin Like Growth IMMUNO Refer to Guide to Services for Reference
Liaison® XL Chemiluminescence assay µg/L
Factor 1 CHEM Intervals
Age Sex Iron TIBC
< 18 y Male 5-25 40-77 TIBC is a
Iron -Blood (& µmol/
DSC Cobas 8000 Colorimetric assay < 18 y Female 5-25 40-77 calculation
TIBC) L
18 y – 150 y Male 8-30 40-80 (DSC only)
18 y – 150 y Female 6-28 40-80
Iron - Overdose Refer to Overdose Iron
Agilent 7890B Gas
mmol/
Isopropanol FMC Chromatograph Gas Chromotography No Reference Interval
L
analyzer
K/L
Ketone Screen -
FMC RRL Precision Xtra Meter Amperometry Negative mmol/L
Blood
Ketone - Urine Go to: Urinalysis
FMC Cobas 8000
Colorimetric Assay
Lactate - Blood RRL Cobas 6000 0.5-2.2 mmol/L
HCTL
Vitros Colorimetric assay
CRL
Page 26 of 48

Established by
CLS Hematology
FMC RRL GEM 4000 Amperometric Division
This analyte is
Lactate – Blood
< 2.0 mmol/L part of a panel test
(Blood Gases)
***VHF patients
***ACH only
i-STAT System Lactate oxidase + amperometry
***SHC i-STAT CG4+
Cartridge
FMC Cobas 8000 Age Value
<3d 1.1-6.7
Lactate, CSF Colorimetric Assay mmol/L
RRL Cobas 6000 3-10 d 1.1-4.4
10 d – 150 y 1.1-2.4
DSC FMC Cobas 8000 Age Value
UV assay 1d–5y 125-320
Lactate RRL Cobas 6000
Dehydrogenase (LD) 6 – 10 y 125-300 U/L
- Blood HCTL 11 – 15 y 115-260
Vitros Multiple-point rate assay slide
CRL
16 – 150 y 100-235
Lactate DSC FMC Cobas 8000
UV assay No Reference Interval U/L
Dehydrogenase - CSF RRL Cobas 6000
Lactate
Dehydrogenase - DSC Cobas 8000 UV assay No Reference Interval U/L
Fluid
A deficiency of lactase, or a
malabsorption problem, is shown by a
Lactose Tolerance DSC Cobas 8000 Hexokinase-UV assay flat or almost flat curve, with the rise in mmol/L
glucose over the fasting level being less
than 1.1 mmol/L
DSC Calculation using results from Age Value
LDL Cholesterol,
Calculation CHOL, TRIG and HDL < 18 y < 2.84 mmol/L
Calculated BMSH cholesterol. 18 - 150 y 0.00 – 3.40
Refer to CH10-
Leflunomide Liquid Chromatography with 2.15 Leflunomide
Ana Tox Agilent LC 1100 No reference interval µg/mL
metabolite DAD Metabolite –
HPLC DAD
Leukocytes - Urine Refer to Urinalysis
Page 27 of 48

DSC FMC Cobas 8000
Enzymatic Colorimetric assay Age Value
Lipase RRL Cobas 6000 < 16 y 0-60 U/L
HCTL 16-150 y 0-80
Vitros Two-point rate assay
CRL
DSC FMC Cobas 8000
Lipase - Fluid Enzymatic colorimetric assay No Reference Interval U/L
RRL Cobas 6000
Lipoprotein (a) DSC Cobas c701-c502 Immunoturbimetric 0.00-0.30 g/L
FMC Cobas 8000 Colorimetric assay Therapeutic range:
Lithium 0.80 - 1.50 (acute therapy) mmol/L
HRH Vitros Colorimetric assay 0.60 - 1.20 (chronic therapy)
Female
1d – 10y 0-6
Follicular 1-13
Luteal 1-17
Luteinizing Hormone `Chemiluminescent sandwich Mid-cycle peak 8-76
DSC Cobas 8000 IU/L
(LH) principle technology Post-menopause 16-54
Male
1 d – 10 y 0-6
11 y – 70 y 1-9
71 y – 150 y 3-35
M
DSC FMC Cobas 8000 Colorimetric method with
RRL Cobas 6000 Xylidyl Blue
BMSH
Magnesium - Blood VCH 0.65-1.05 mmol/L
CMGH
DDHS
HRH
SDHS
Colorimetric method with
Magnesium - Stool FMC Cobas 8000 No Reference Interval mmol/L
Xylidyl Blue
Colorimetric method with
Magnesium - Urine DSC Cobas c701-c502 3.0- 5.5 mmol/ day
Xylidyl Blue
Page 28 of 48

Refer to GL-15.02
Meat Fibres, DSC Fat Globules and
Manual Microscopic < 6 meat fibers/hpf
Qualitative – Stool Urinalysis Meat Fibres -
Stool
Refer to
CH10-1.24
Metanephrines - Agilent LC 1100 with Plasma Normetanphrine <0.90
ANA TOX Tandem Mass Spectrometry nmol/L Metanephrine in
Plasma MS 6410 Plasma Metanephrine <0.50
Plasma – 6410
6460
Normetanephrine (24 hour)
Age Male Female
<3y Not Available
3y-8y 0.2-0.9 0.2-0.8
9 y - 12 y 0.5-2.3 0.3-1.5
13 y - 17 y 0.5-2.5 0.3-1.6
µmol/d
18 y - 29 y 0.6-2.1
30 y - 39 y 0.6-2.3
40 y - 49 y 0.6-2.5
50 y - 59 y 0.7-2.6
60 y - 69 y 0.8-2.8
70 y - 150 y 0.8-3.1
Random Normetanephrine
Refer also to
<3y 75-584
Metanephrines - Agilent LC 1100 with µmol/ mol CH10-1.22
ANA TOX Tandem Mass Spectrometry 3y-8y 57-443
Urine MS 6410 Ur Creat Metanephrine in
9y - 12 y 33-255
Urine- 6410 6460
13y - 17 y 23-176
Metanephrine (24 hour)
Age Male Female
<3y Not Available
3 y -8 y 0.2-0.5 0.1-0.7 µmol/d
9y - 12 y 0.3-1.0 0.2-0.6
13 y - 17 y 0.4-1.1 0.2-0.9
18 y- 150 y 0.2-1.3 0.2-0.9
Random Metanephrine
<3y 47-240
µmol/ mol
3y-8y 37-191
Ur Creat
9 y - 12 y 24-120
13 y - 17 y 17-88
Methadone
Metabolite Screen - Refer to CH-2.22 Drugs of Abuse
Urine
Page 29 of 48

Agilent 7890B Gas
Methanol FMC Gas Chromatography No Reference Interval mmol/L
Chromatograph analyzer
Age Values Established by
FMC RRL
<8y No Reference AHS Calgary
CMGH
Methemoglobin Zone Respiratory
DDHS GEM 4000 CO-Oximetry Interval %
(MTHB) Services
HRH
This analyte is
SDHS 8 y – 150 y 0.0 – 1.5 part of a panel test
Therapeutic Level interpretation
dependent on institutional protocols,
type of therapy (intermediate vs high
dose) and individual patient factors.
Homogeneous Enzyme Dosage Time Toxic
Methotrexate ACH Cobas 6000 µmol/L
Immunoassay Low After 1 week > 0.04
High After 24 h > 5.0
Microalbumin Go to Albumin - Urine
Mitochondrial IMMUNO Indirect Immunoflorescence Also known as
Manual Negative
Antibody CHEM Slide Tissue Antibodies
IMMUNO
CHEM
Monogen particle agglutination
Monotest Manual Negative
RRL HEM methodology
HCTL CRL
Mysoline Refer to Primidone
N
Indirect Immunoflorescence
Neutrophil Sprinter-Sprinter XL ANCA Slide Interpretation: negative Titre
Slide
Cytoplasmic IMMUNO
AI
Antibody (ANCA - CHEM Anti-MPO antibody: <1.0
BioPlex Multiplex Flow Assay (Antibody
PR3 & MPO) Anti-PR3 antibody: <1.0
Index)
Nitrite - Urine Go to: Urinalysis
Page 30 of 48

<18 y No Reference Interval
Lipid Profile acceptable limits relative

to dyslipidemia and atherosclerosis
risk:
Total Cholesterol Acceptable < 4.40
DSC Cobas 8000 mmol/L
HDL-C Acceptable > 1.16 mmol/L
LDL-C Acceptable < 2.84 mmol/L
Triglycerides Acceptable < 0.85
mmol/L (0-9 y) OR < 1.02 mmol/L
(10-17 y)
Non-HDL-C Acceptable < 3.10
*18 -150 y 0.0 – 4.2
Low Risk (FRS < 10%)

Treatment advised if LDL-C ≥ 5.0
*For patients 30 y
mmol/L
of age or older, the
Treatment target: > 50% reduction
Framingham Risk
Non – HDL LDL-C
Calculation mmol/L Score (FRS),
Cholesterol
modified for
Intermediate Risk (FRS 10 - 19%)
family history, is
Treatment advised if LDL-C ≥ 3.5
recommended for
mmol/L OR Non-HDL-C ≥ 4.3
risk assessment
mmol/L OR ApoB ≥ 1.2 g/L; Consider
(2016 CCS
treatment for men ≥ 50 and women ≥
Guideline, Can J
BMSH Vitros 60 yrs with one additional CV risk
Cardiol 2016)
factor
Treatment targets: LDL-C < 2.0
FRS Calculation
mmol/L OR decrease by > 50% OR
Resources can be
Non-HDL-C < 2.6 mmol/L OR ApoB
found at
< 0.8 g/L
www.myhealth.alb
erta.ca/Alberta/Pa
High Risk (FRS ≥ 20% or presence
ges/Heart-
of high risk features)
Disease-Risk-
Treatment advised in all patients
Calculator.aspx
Treatment targets: LDL-C < 2.0
mmol/L OR decrease by > 50% OR
Non-HDL-C < 2.6 mmol/L OR ApoB
< 0.8 g/L
nmol/
IMMUNO Male and pre-menopausal women:
N-Telopeptide (NTx) Triturus Enzyme immunoassay mmol
CHEM 5.0 - 65.0
creat
Page 31 of 48

IMMUNO
Cobas e601
CHEM
FMC Cobas 8000 Chemiluminescence assay
PLC RGH
Cobas 6000
SHC
ACHC
Immunoassay sandwich method
CCHC
NT-proBNP mini VIDAS with a final fluorescent detection 0 - 300 ng/L
BMSH
(ELFA)
OHWC
CLH
Two-site sandwich assay, solid
CMGH
phase Radial Partition
DDHS Stratus CS
Immunoassay (RPIA)
HRH OGH
technology
SDHS VCH
Nuclear Antibody IMMUNO Indirect Immunoflorescence
Sprinter-Sprinter XL Negative Titre
(ANA) CHEM Slide
Page 32 of 48
O
FMC RRL *Established by
BMSH AHS Calgary
Age *Value
CMGH GEM 4000 Co-Oximetry Zone Respiratory
DDHS HRH Services
Arterial < 29 d 92 - 96
SDHS
29 d – 150 y 88 - 100 This analyte is
O2 Saturation = Oxyhemoglobin part of a panel test
/ (oxyhemoglobin +
**ACH Venous < 29 d 85-88
i-STAT System deoxyhemoglobin) **VHF patients
**SHC 29 d – 150 y 50-80
Calculated from pO2, pCO2 and only
O2 Saturation pH.
%
(Oxyhemoglobin) i-STAT CG4+
Cartridge
CLH GEM 3500 Derived analyte Assumes normal
Arterial 83 – 108 hemoglobin
affinity for
Venous 60 – 85 oxygen and
O2 Saturation = Oxyhemoglobin normal amounts
/ (oxyhemoglobin + of dysfunctional
OGH Capillary < 70
i-STAT System deoxyhemoglobin) hemoglobins
VCH
Calculated from pO2, pCO2 and (carboxy, met and
pH. sulfhemoglobin)
Effective January
DSC 1st, 2014 this test
Urinalysis is only available
The Hemoccult test is based on for hospital
FMC RRL the oxidation of guaiac by inpatients and
Occult Blood - Stool Manual Negative
Chem hydrogen peroxide to a blue emergency
colored compound patients.
DDHS HRH Refer to GL-15.01
SDHS Occult Blood in
Stool
Electrochemiluminescent
Oncology Cortisol FMC Cobas 8000 No Reference Interval nmol/L
immunoassay
Opiate Screen –
Urine
Refer to CH-
OGAP = measured osmolality – mmol/k
Osmolal Gap FMC RRL Calculation No Reference Interval 43.07 Osmometer
calculated osmolality g
Reporting
Page 33 of 48

Advanced Osmometer
Osmolality – Blood 280 - 300
Model 2020
Osmolality – Fluid FMC RRL No Reference Interval
Advanced Micro- Advanced Osmometers
mmol/k
Osmolality - Urine Osmometer 3320 determine osmolality by means 50 - 1200 g
of freezing point measurement.
Advanced Automated
Osmolality – Stool FMC Osmometer Model 284 - 430
A2O
Age Sex Iron
< 18 y Male 5-25
Overdose Iron ACH Cobas 6000 Colorimetric assay < 18 y Female 5-25 µmol/L
18 y – 150 y Male 8-30
18 y – 150 y Female 6-28
Oxycodone Screen -
Refer to CH-2.22 Drugs of Abuse
Urine
P
Parathyroid Hormone IMMUNO
Liaison XL Chemiluminescence (CLIA) 7 - 37 ng/L
(PTH) CHEM
Parathyroid Hormone FMC Cobas 8000
Chemiluminescence (CLIA) No Reference Interval ng/L
- Intra Operative RGH Cobas 6000
Parathyroid Hormone
FMC Cobas 8000 Testing done
- Total
Chemiluminescence (CLIA) No Reference Interval ng/L post-op of a total
Thyroidectomy
RGH Cobas 6000 thyroidectomy
(PTHTT)
Also known as
IMMUNO
Parietal Cell Antibody Manual Indirect Immunoflorescence Slide Negative Tissue
CHEM
Antibodies
Page 34 of 48

FMC RRL
BMSH This analyte is
CMGH GEM 4000 Potentiometric sensor Arterial 30 - 40 part of a panel
DDHS Venous 36 - 46 test
HRH SDHS Capillary 35 - 45
pCO2 - Blood **ACH Measured by direct mm Hg i-STAT CG4+
i-STAT System Cartridge
**SHC potentiometry - Nernst equation
CLH GEM 3500 Potentiometric Arterial 35 - 48
**VHF
OGH Measured by direct Venous 40 - 52
i-STAT System patients only
VCH potentiometry - Nernst equation Capillary 38 - 45
FMC Age *Value *Established by
RRL AHS Calgary
BMSH Arterial < 29 d 7.35 -7.45 Zone respiratory
CMGH GEM 4000 Potentiometric sensor 29d-150y 7.36 – 7.44 Services
DDHS
HRH Venous All 7.30 – 7.40 This analyte is
SDHS part of a panel
pH - Blood
**ACH Measured by direct potentiometry Capillary < 29 d 7.32 – 7.42 test
i-STAT System
**SHC - Nernst equation 29d-150y 7.32 – 7.38
**VHF patients
CLH GEM 3500 Potentiometric sensor Arterial 7.35-7.45 only
Venous 7.31-7.41
OGH Measured by direct potentiometry Capillary 7.35-7.45 i-STAT CG4+
i-STAT System
VCH - Nernst equation Cartridge
ACH, PLC and
RGH - Deliver
FMC Potentiometric sensor which is
pH - CSF GEM 4000 No Reference Interval specimens to
SHC located in a cartridge
Respiratory
Therapy
ColorpHast reagent strips use an
Refer to CH-
indicator combination to produce
pH - Other Fluid FMC RRL Manual No Reference Interval 6.50 pH in Fluid
distinctive color changes for pH
– FMC RRL
0-14.
Respiratory -
Potentiometric sensor which is
pH - Pleural Fluid ACH FMC GEM 4000 No Reference Interval
located in a cartridge
PLC RGH
DSC Refer to GL-
pH – Stool Manual Multistix reagent strip for pH 7.0-8.0
Urinalysis 15.03 pH - Stool
pH - Urine Go to Urinalysis
Page 35 of 48

Phenobarbitol ACH Cobas 6000 Microparticles in Solution Therapeutic range: 65 - 170 µmol/L
(KIMS) Immunoassay
FMC Cobas 8000 Kinetic Interaction of Therapeutic Range:
Microparticles in Solution
Phenytoin RRL Cobas 6000 <4m 25 - 55 µmol/L
(KIMS) Immunoassay
4 m – 150 y 40 - 80
HRH SDHS Vitros Multiple-point immuno-rate assay
Phenytoin, Free FMC Cobas 8000 Microparticles in Solution Therapeutic range: 4.0-8.0 µmol/L
(KIMS) Immunoassay
Age Value
DSC FMC Cobas 8000
1 d – 30 d 1.60-3.10
Molybdate UV assay
1m–4m 1.50-2.60
RRL Cobas 6000
5 m – 12 m 1.30-2.20
Phosphate - Blood SMCHC 1y–4y 1.10-2.10 mmol/L
BMSH 5y–9y 1.10-2.00
CMGH Vitros Colorimetric 10 y – 14 y 1.00-1.90
DDHS HRH 15 y – 19 y 0.90-1.60
SDHS 20 y – 150 y 0.80-1.50
DSC FMC Cobas 8000
Phosphate - Fluid Molybdate UV assay No Reference Interval mmol/L
RRL Cobas 6000
Phosphate – Urine DSC Cobas c701-c502 Photometric (Molybdate UV) 16.0 - 48.0 mmol/d
Phosphate/ Creatinine
Ratio, Urine
Random Urine Risk Association to
PBG Range Acute Intermittent
(µmol/L) Porphyria
Refer to
Agilent 8453 Anion Exchange with Erlich's 3-9 No Risk
Porphobilinogen ANA TOX µmol/L CH-45.03
Spectrophotometer Color Reaction 10-88 Moderate Risk Porphobilinogen
89-176 High Risk
> 176 Highest Risk
Page 36 of 48

FMC Age *Value
*Established by
RRL BMSH
AHS Calgary
CMGH GEM 4000 Amperometry Arterial < 29 d 50 - 70
Zone respiratory
DDHS HRH 29d-150y 70 - 88
Services
SDHS
Venous All 30 - 50
This analyte is
**ACH
i-STAT System Amperometry part of a panel
pO2 - Blood **SHC Capillary < 29 d 30 - 40
test
29d-150y 35 - 45
**VHF patients
CLH GEM 3500 Amperometry Arterial 70 - 90 only
Venous All 30 - 50
Capillary 40 - 100 i-STAT CG4+
OGH
i-STAT System Amperometry Cartridge
VCH
Coproporphyrin 24-190 Refer to CH-
Porphyrin, Agilent 8453 Multicomponent Second nmol/ 24
ANA TOX 45.04
Quantitative - Urine Spectrophotometer Derivative Analysis Uroporphyrin < 36 hours
Porphyrins
FMC DSC Cobas 8000 Indirect potentiometry assay Age Value
RRL Cobas 6000 using an ion-selective electrode
Less than 4 m 4.0 - 6.2
Potassium - Blood HCTL CRL Vitros Direct potentiometric assay mmol/L
4 – 11 m 3.7 - 5.6
***ACH Ion-selective electrode (ISE) 1 – 150 y 3.3 - 5.1 ***VHF patients
i-STAT System
***SHC potentiometry only
*Established by
CLS Chemistry
Division through
literature review
Potassium – Blood (Whole Blood
FMC RRL GEM 4000 Potentiometric sensor 3.3 – 5.1* mmol/L
(Blood Gases) Reference
Interval)
This analyte is
part of a panel
test
DSC FMC Cobas 8000 Indirect potentiometric assay
Potassium - Fluid No Reference Interval mmol/L
RRL Cobas 6000 using an ion-selective electrode.
Indirect potentiometric assay

Potassium – Stool FMC Cobas 8000 No Reference Interval mmol/L
using an ion-selective electrode.
Page 37 of 48

DSC Cobas c701-c502
Indirect potentiometric assay 24 hour: 26 - 123 mmol/d
Potassium - Urine FMC Cobas 8000
RRL Cobas 6000 Random: No Reference Interval mmol/L
Prealbumin DSC Cobas c701-c502 Immunoturbidimetric assay 0.2 - 0.4 g/L
Enzyme Multiplied Immunoassay
Primidone ACH Cobas 6000 Therapeutic range: 23 - 55 µmol/L
Technique
Female:
< 12 y 0.0 - 0.5
Competitive chemiluminescent Follicular 0.0 - 2.8
Progesterone DSC Cobas 8000 nmol/L
assay Luteal 5.0 - 76.0
Post-menopause 0.0 - 0.5
Male: 0.0 - 0.5
Chemiluminescent sandwich Male Female
Prolactin DSC Cobas 8000 µg/L
technology 0-15 0-25
Age Male
1 d – 49 y 0.00-2.50
Prostate Specific Chemiluminescent sandwich 50 y – 59 y 0.00-3.50
DSC Cobas 8000 µg/L
Antigen (PSA) assay
60 y – 69 y 0.00-4.50
70 y – 150 y 0.00-6.50
In males with total PSA concentrations of 4-
10 µg/L the possibility of finding cancer on
needle biopsy by age in years is:
% Free
Prostate Specific 50-59 y 60-69 y 70-150 y
Chemiluminescent sandwich PSA
Antigen, DSC Cobas 8000
assay < 11 % 49 % 58 % 65 %
Fractionated/Free
11-18 % 27 % 34 % 41 %
19-25 % 18 % 24 % 30 %
> 25 % 9% 12 % 16 %
Page 38 of 48

DSC FMC Cobas 8000 Colorimetric assay utilizing biuret
RRL Cobas 6000 reagent
Age Value
BMSH
1 – 365 d 54-79
Protein, Total - Blood CMGH g/L
Vitros Colorimetric assay 1–4y 59-79
DDHS HRH
SDHS 5 – 150 y 63-80
***ACH ***VHF patients
***SHC only
Gen. 3 turbidimetric endpoint
DSC Cobas c701-c502 assay utilizing benzethonium
chloride. Age Value
Protein, Total - CSF FMC Cobas 8000 < 31 d 0.14-1.12 g/L
Turbidmetric Assay 31 d – 150 y 0.15-0.45
RRL Cobas 6000
BMSH Vitros Colorimetric assay
DSC FMC Cobas 8000 Colorimetric assay utilizing biuret
Protein, Total - Fluid No Reference Interval g/L
RRL Cobas 6000 reagent
Protein, Qualitative
Go to Urinalysis
Urine
Gen. 3 turbidimetric endpoint
Protein, Total
DSC Cobas c701-c502 assay utilizing benzethonium 0.00 - 0.15 g/24 hours
Quantitative - Urine
chloride.
Protein/Creatinine
Ratio, Urine
Protein
Electrophoresis - Go to Electrophoresis, Protein – Serum or Electrophoresis, Protein - Urine
Serum and Urine
S-butyrylthiocholine iodide using
Pseudocholinesterase dibucaine as an inhibitor. To Refer to CH-
(Anaesthetic) determine the dibucaine number 55.07 Cobas
DSC Calculation 5.3 - 12.9 KU/L
(See also Dibucaine (DN), cholinesterase activity is c701-c502
number) measured with and without Calculations
dibucaine.
Page 39 of 48
R
Stool reducing
substances test
is restricted to
Acute Care sites
The Benedict’s Solution detects
Reducing Substances – (NICU, PICU)
reducing substances in urine
Stool ACH Manual Negative and pediatric
using the color changes of the
(STRED) gastroenterolog
copper ions.
y inpatients at
Alberta
Children’s
Hospital
In healthy adults (18-65 y) with normal
IMMUNO salt intake:
Renin Liaison XL Chemiluminescence mIU/L
CHEM 4.4 - 46.0 upright at least 30 minutes
2.8 - 40.0 supine at least 30 minutes
Also known as
IMMUNO Indirect Immunoflorescence
Reticulin Antibody Manual Negative Tissue
CHEM Slide
Antibodies
Rheumatoid Factor,
Quantitative - Blood DSC Cobas c701-c502 Immunoturbidimetric assay 0-20.0 kU/L
(RF)
Rheumatoid Factor,
Qualitative - Blood BMSH Manual Pulse Scientific RF Latex Kit Negative
(RF)
Rheumatoid Factor -
DSC Cobas c701-c502 Immunoturbidimetric assay No Reference Interval kU/L
Fluid
Chemiluminescent Microparticle
Rubella Antibodies, IMMUNO >10 kIU/L indicates past exposure to
Architect Immunoassay technology kIU/L
Immune Status (IgG) CHEM Rubella Virus
(CMIA)
S
FMC Cobas 8000
UV assay
RRL Cobas 6000
SMCHC
CCHC Therapeutic range: 0.70 - 1.80
Salicylate mmol/L
BMSH (anti-inflammatory)
CMGH CLH
HRH OGH
SDHS VHC
Page 40 of 48

Sex Hormone Binding IMMUNO Male Female
Siemens Immulite 2000 Chemiluminescent assay nmol/L
Globulin CHEM 10-55 20-100
Agilent LC with ABsciex
Sirolimus ANA TOX Tandem Mass Spectrometry factors such as transplant type, time post µg/L
MS
immunosuppressive therapy.
Sodium - Blood HCTL CRL Vitros Direct potentiometric assay 133 - 145 mmol/L
***ACH Ion-selective electrode (ISE) ***VHF
i-STAT System
***SHC potentiometry patients only
*Established by
CLS Chemistry
Division
through
literature review
Sodium – Blood (Whole Blood
FMC RRL GEM 4000 Potentiometric Sensor 133-145* mmol/L
(Blood Gases) Reference
Interval)
This analyte is
part of a panel
test
Sodium - Fluid No Reference Interval mmol/L
Indirect potentiometric assay
Sodium - Stool FMC Cobas 8000 No Reference Interval mmol/L
DSC Cobas c701-c502
Indirect potentiometry assay using 24 hour: 43 - 217 mmol/ day
Sodium - Urine FMC Cobas 8000
an ion-selective electrode.
RRL Cobas 6000 Random: No Reference Interval mmol/L
Fractional Sodium is a calculation
which includes
Refer to CH-
serum sodium (mmol/L),
Sodium, Fractional DSC FMC 2.41 Fractional
Calculation serum creatinine (mmol/L), No Reference Interval mmol/L
Excretion RRL Sodium –DSC
urine sodium (mmol/L) and
FMC RRL
urine creatinine (mmol/L)
concentrations in the calculation.
Page 41 of 48

Refer to CH-
Smooth Muscle IMMUNO
Manual Indirect Immunoflorescence Slide Negative 6.43 Tissue
Antibody CHEM
Antibodies
Refer to CH-
The refractometer measures the 6.53 Specific
Specific Gravity - Fluid FMC Manual No Reference Interval
refractive index of liquids. Gravity in Fluid
- FMC
Specific Gravity - Urine Go to: Urinalysis
Sweat Normal Range
Chloride <7m 0-29
7 m – 150 y 0-39
The Chlorochek Chloridometer is Marginal Range
a dedicated coulometric- mmol/L
Wescor Chlorochek <7m 30-59
Sweat Chloride ACH amperometric titrator designed to
Chloridometer 7 m – 150 y 40-59
determine chloride ion
concentrations in sweat samples. Values Consistent with
Cystic Fibrosis
60-160
Volume > 14 µL
Abbott Architect i Syphilis TP is
IMMUNO based on the Chemiluminescent
Syphilis - Blood Architect Negative no units
CHEM Microparticle Immunoassay
(CMIA) technology.
Page 42 of 48
T
Age Male/Female
4 d - 30 d 3.0-8.1
31 d – 1 y 2.4-9.8
Competitive chemiluminescent
T3, Free DSC Cobas 8000 2y–6y 3.0-9.1 pmol/L
technology
7 y – 11 y 4.1-7.9
12 y – 17 y 3.5-7.7
18 y - 150 y 3.5-6.5
Age Male/Female
Competitive chemiluminescent 1-365 d 1.5-4.2
T3, Total DSC Cobas 8000 nmol/L
technology 1-15 y 1.4-3.3
16-150 y 1.1-2.8
Competitive chemiluminescent
T4, Free DSC Cobas 8000 10.0 - 25.0 pmol/L
technology
Non-pregnant
IMMUNO Pregnant
T4, Total Cobas e601 Chemiluminescence assay (Male and Female) nmol/L
CHEM
59-154 85-190
Agilent LC with
Tacrolimus ANA TOX Tandem Mass Spectrometry factors such as transplant type, time post µg/L
ABsciex MS
immunosuppressive therapy.
Page 43 of 48

Refer to CH-
Testosterone, IMMUNO Adult Male Adult Female
Calculation nmol/L 37.07 Immulite
Bioavailable CHEM 4.0-16.0 0.1-1.0
Calculations
IMMUNO Adult Male Adult Female
Testosterone, Free Calculation pmol/L
CHEM 175-700 2-30
Age Male Female
1d-9y 0.0 - 1.0 0.0 - 1.0
Tanner
0.0 - 1.0 0.0 - 1.0
Stage 1
Tanner
0.0 – 15.0 0.0 - 1.0
Stage 2
Competitive chemiluminescent 10-17 Tanner
Testosterone, Total DSC Cobas 8000 2.2 - 27.0 0.0 - 1.0 nmol/L
technology y Stage 3
Tanner
6.2 - 26.5 0.0 - 1.0
Stage 4
Tanner
6.5 - 29.0 0.2 - 1.3
Stage 5
18y - 150 y: 8.0 - 29.0 0.5 - 2.0
Kinetic Interaction of Age Value
Theophylline FMC Cobas 8000 Microparticles in Solution < 32 d 25-110 µmol/L
(KIMS) Immunoassay 32 d – 150 y 55-110
Also known as
The thiocyanate present in the
Sodium
filtrate is reacted with ferric Thiocyanate levels up to 0.5 are seen with
Nitroprusside,
Agilent 8453 nitrate in a 0.5 M nitric acid nitroprusside therapy.
Thiocyanate ANA TOX mmol/L Nipride
Spectrophotometer medium. The absorbance of ferric Thiocyanate levels greater than 1.4 are
Refer to CH-
thiocyanate is measured at 470 reportedly toxic.
45.05
nm.
Thiocyanate
IMMUNO
Thyroglobulin Cobas e601 Chemiluminescence assay 0 - 50 µg/L
CHEM
Thyroglobulin Antibody
IMMUNO
(Anti-Thyroglobulin - Cobas e601 Chemiluminescence assay 0 - 35 KIU/L
CHEM
Anti-TG)
Page 44 of 48

Also known as
Anti-Thyroid
Antibodies,
Thyroid
Thyroid Peroxidase IMMUNO Competitive chemiluminescent Antibodies,
Cobas e601 0-34 KIU/L
Antibody CHEM technology Anti-Microsomal
Antibodies,
Anti-Thyroid
Peroxidase, and
Anti-TPO
Age Male/Female
1d 11.00-36.00
Thyroid Stimulating Chemiluminescent sandwich
DSC Cobas 8000 2d 8.00-20.00 mIU/L
Hormone (TSH) assay
3d 1.00-11.00
4 d - 150 y 0.20-4.00
Tissue IMMUNO Component of
BioPlex Multiplex Flow Assay < 15.0 kIU/L
Transglutaminase IgA CHEM Celiac Screen
Interpretative ranges:
Tissue IMMUNO Component of
BioPlex Multiplex Flow Assay < 15 – Negative kIU/L
Transglutaminase IgG CHEM Celiac Screen
≥ 15 – Positive
Homogenous enzyme Pre-dose Post-dose
Tobramycin FMC Cobas 8000 mg/L
immunoassay. 0.0- 1.9 5.0-10.0
Transferrin
All ages 2.0-3.6 g/L
Transferrin - Blood DSC Cobas c701-c502 Immunoturbidimetric Transferrin Saturation Index
<18 y 0.20 – 0.55
18 y -150 y 0.15 – 0.50
Age Male/Female DSC performs
DSC FMC Cobas 8000 < 18 y < 1.50 routine
Triglycerides
Acceptable limit testing
relative to Triglycerides
Enzymatic colorimetric assay testing also
dyslipidemia and
Triglycerides - Blood atherosclerosis risk < mmol/L performed on
ACH Cobas 6000 adults and
0.85 mmol/L (0-9 y)
OR < 1.02 mmol/L pediatric patients
(10-17 y) at ACH/FMC.
(neonatal
BMSH Vitros Enzymatic, end point 18 y - 150 y 0.00 – 1.70 triglycerides -
NTRIG)
Page 45 of 48

DSC FMC Cobas 8000
Triglycerides - Fluid Enzymatic colorimetric assay No Reference Interval mmol/L
ACH Cobas 6000
One-step immunoassay sandwich
HCTL BMSH
mini VIDAS method with fluorescent detection < 0.01
OHWC
(ELFA)
CLH DDHS
Troponin I Two-site immunoenzymatic µg/L
OGH SDHS CS Stratus 0.00 - 0.06
(sandwich) assay
VCH
HRH CMGH Access Two-site sandwich assay 0.01 - 0.06 **VHF Patients
**SHC Triage Meter Fluorescence immunoassay <0.02 Only
DSC FMC Cobas 8000 Normal: < 15
Troponin T, High Electrochemiluminescence
PLC RGH Borderline Elevation: 15 - 109 ng/L
Sensitivity Cobas 6000 immunoassay
SHC Clear Elevation: > 109
U
DSC FMC Cobas 8000 Age Male Female
Enzymatic colorimetric
RRL Cobas 6000 1 d-17 y 140-380 140-380
BMSH CLH Alternate Name -
Urate - Blood 18 y - 50 y 210-490 140-350 µmol/L
CMGH Uric Acid
Vitros Colorimetric assay 51 y - 69 y 210-490 160-410
DDHS HRH 70 y - 150 y 210-490 160-450
SDHS
DSC FMC Cobas 8000
Urate - Fluid Enzymatic colorimetric No Reference Interval µmol/L
RRL Cobas 6000
Enzymatic colorimetric test with
uricase and 4-aminoantipyrine. In
Urate - Urine DSC Cobas c701-c502 the initial step, uric acid is 1.5 - 4.5 mmol/day
oxidized in a reaction catalyzed
by uricase.
Urate/ Creatinine Ratio,
Urine
DSC FMC Cobas 8000 Kinetic UV assay
Age Male Female
Kinetic UV assay with urease and
RRL Cobas 6000 1-365 d 1.5-7.0 1.5-7.0
glutamate dehydrogenase **VHF patients
Urea - Blood Colorimetric assay utilizing 1-14 y 2.0-7.0 2.0-7.0 mmol/L
HCTL CRL Vitros only
urease. 15-54 y 3.0-7.5 2.0-7.0
**ACH Kinetic UV assay with urease and 55-150 y 3.0-9.0 2.5-8.5
Piccolo Express
**SHC glutamate dehydrogenase
DSC FMC Cobas 8000
Urea - Fluid Kinetic UV assay No Reference Interval mmol/L
RRL Cobas 6000
Page 46 of 48

NADH to NAD oxidation
Urea - Urine DSC Cobas c701-c502 reaction measured No Reference Interval mmol/L
photometrically
Urea Breath Test for AP2003/ SerCon
DSC Mass Spectrometry No Reference Interval
Helicobacter pylori ABCA2
Macroscopic
iRICELL
Specific gravity 1.005 – 1.030
(iChem VELOCITY –
DSC pH 5.0-8.5
macroscopic / iQ-200 –
Urinalysis Leukocytes Neg
microscopic)
Nitrite Neg
FMC PLC
iChem 100 Macroscopic urinalysis includes a Protein (g/L) Neg
RGH SHC Refer to General
(macroscopic) physical description of the Glucose (mmol/L) Neg
Chem Lab Procedure
(backup and low volume specimen and performance of a Ketones Neg
samples) Manual Sections
group of chemical screening tests, Blood Neg
Urinalysis 10 Urinalysis
which may indicate the need for a
Microscopic 12 iRICELL
subsequent microscopic
WBC < 16 y 0-2/hpf 13 Clinitek
examination to identify and
ACH Chem Clinitek Advantus 16 – 150 y 0-5/hpf 21 iChem100
quantitate specific elements.
(macroscopic) RBC < 16 y 0-2/hpf
HCTL 16 – 150 y 0-5/hpf
Microscope Granular casts Neg
CRL (microscopic) WBC casts Neg
RBC casts Neg
Waxy casts Neg
Refer to General
The coupling reaction of
Urobilinogen, DSC Laboratory
iRICELL urobilinogen with a stable <17 µmol/L
Qualitative Urinalysis Section 10
diazonium salt in buffer
Urinalysis
V/W
Homogeneous enzyme
Valproate (Depakene) FMC Cobas 8000 Therapeutic range: 350 - 700 µmol/L
immunoassay.
FMC Cobas 8000 Kinetic Interaction of
Vancomycin Microparticles in Solution Pre-dose: 10.0- 20.0 mg/L
RRL Cobas 6000 (KIMS) Immunoassay
IMMUNO The viscosity assay is flow time
Viscosity Manual 1.4 - 1.8 no units
CHEM relative to water
Page 47 of 48

Age Value
<1y 0.5 to 1.8 Alternate Name
1y-6y 0.7 to 1.5 – Retinol
Vitamin A ANA TOX Agilent LC 1100 µmol/L Refer to CH10-
DAD 7 y - 12 y 0.9 to 1.7 2.20 Vitamin A
13 y - 17 y 0.9 to 2.5 and E
17 y - 150 y 1.5 to 3.5
Competitive Chemiluminescence
Vitamin B12 DSC Cobas 8000 155 - 700 pmol/L
Assay
Vitamin D, 1,25 IMMUNO
Liaison XL Chemiluminescence (CLIA) 60-208 pmol/L
DiHydroxy CHEM
Severe Deficiency: < 25
IMMUNO Moderate to Mild Deficiency: 25-80
Vitamin D, 25 Hydroxy Liaison XL Chemiluminescence (CLIA) nmol/L
CHEM Optimum Levels: 80-200
Toxicity Possible: > 250
Age Value
<1y 5 to 20 Refer to CH10-
Vitamin E ANA TOX Agilent LC 1100 1-6y 7 to 21 µmol/L 2.20 Vitamin A
DAD
7 - 12 y 10 to 21 and E
13-150 y: 12 to 45
Refer to CH-
6.51 Water
Water Deprivation ACH FMC No Reference Interval
Cobas analyzer, Deprivation-
Two “panels” of tests are
osmometer and ACH FMC
ordered
urinalysis analyzer Refer to CH-
Water Load FMC No Reference Interval 6.52 Water
Load-FMC
RELATED DOCUMENTS
CH-2.11 Critical Values for Acute Care Community and Rural Sites
CH-2.22 Drugs of Abuse Testing
CH-2.24 Half-Life and Time to Steady State
HE-1.11 Hematology Critical Values or
HE-1.21 CBC Reference Intervals
Page 48 of 48

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21 Analyte Reference Interval Instrumentation Methodology Chart

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Analyte Site Instrumentation Method Reference Interval Units Notes

Analyte Site Instrumentation Method Reference Interval Units Notes

Analyte Site Instrumentation Method Reference Interval Units Notes

Analyte Site Instrumentation Method Reference Interval Units Notes

Analyte Site Instrumentation Method Reference Interval Units Notes

Acceptable limit relative to dyslipidemia

If result is ≥ 1.2 g/L :

If result is < 1.2 g/L:

Analyte Site Instrumentation Method Reference Interval Units Notes

Analyte Site Instrumentation Method Reference Interval Units Notes

Analyte Site Instrumentation Method Reference Interval Units Notes

Analyte Site Instrumentation Method Reference Interval Units Notes

Analyte Site Instrumentation Method Reference Interval Units Notes

Analyte Site Instrumentation Method Reference Interval Units Notes

18 to 150 y Desirable < 5.17

Analyte Site Instrumentation Method Reference Interval Units Notes

Analyte Site Instrumentation Method Reference Interval Units Notes

Analyte Site Instrumentation Method Reference Interval Units Notes

Analyte Site Instrumentation Method Reference Interval Units Notes

Analyte Site Instrumentation Method Reference Interval Units Notes

Analyte Site Instrumentation Method Reference Interval Units Notes

Analyte Site Instrumentation Method Reference Interval Units Notes

Analyte Site Instrumentation Method Reference Interval Units Notes

CRL Vitros Glucose Oxidase - Colourimetric Gestational diabetes mellitus is

Analyte Site Instrumentation Method Reference Interval Units Notes

Fasting Specimen (GTTNP):

Diagnosis of Gestational Diabetes Mellitus:

FMC RRL Refer to:

Analyte Site Instrumentation Method Reference Interval Units Notes

Analyte Site Instrumentation Method Reference Interval Units Notes

Analyte Site Instrumentation Method Reference Interval Units Notes

Analyte Site Instrumentation Method Reference Interval Units Notes

Analyte Site Instrumentation Method Reference Interval Units Notes

Analyte Site Instrumentation Method Reference Interval Units Notes

Analyte Site Instrumentation Method Reference Interval Units Notes

Lipid Profile acceptable limits relative

Low Risk (FRS < 10%)

Analyte Site Instrumentation Method Reference Interval Units Notes

Analyte Site Instrumentation Method Reference Interval Units Notes

Analyte Site Instrumentation Method Reference Interval Units Notes

Analyte Site Instrumentation Method Reference Interval Units Notes

Analyte Site Instrumentation Method Reference Interval Units Notes

Indirect potentiometric assay

Analyte Site Instrumentation Method Reference Interval Units Notes

Analyte Site Instrumentation Method Reference Interval Units Notes

Analyte Site Instrumentation Method Reference Interval Units Notes

Analyte Site Instrumentation Method Reference Interval Units Notes

Analyte Site Instrumentation Method Reference Interval Units Notes

Analyte Site Instrumentation Method Reference Interval Units Notes

Analyte Site Instrumentation Method Reference Interval Units Notes

Analyte Site Instrumentation Method Reference Interval Units Notes

Analyte Site Instrumentation Method Reference Interval Units Notes

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