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Product Specifications

This document provides product specifications for a biotin-SP-conjugated AffiniPure F(ab')2 fragment donkey anti-human IgG, Fcγ fragment specific antibody including the code number, lot number, physical state, size, antibody concentration, suggested dilutions, biotin-SP conjugation, buffer, stabilizer, preservative, storage and rehydration instructions, extended storage instructions after rehydration, purity, antibody specificity, country of origin, and intended use.

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Kartik Bhamidipati
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24 views

Product Specifications

This document provides product specifications for a biotin-SP-conjugated AffiniPure F(ab')2 fragment donkey anti-human IgG, Fcγ fragment specific antibody including the code number, lot number, physical state, size, antibody concentration, suggested dilutions, biotin-SP conjugation, buffer, stabilizer, preservative, storage and rehydration instructions, extended storage instructions after rehydration, purity, antibody specificity, country of origin, and intended use.

Uploaded by

Kartik Bhamidipati
Copyright
© © All Rights Reserved
Available Formats
Download as PDF, TXT or read online on Scribd
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872 W.

Baltimore Pike, West Grove, PA 19390


800-367-5296, 610-869-4024, FAX:610-869-0171
[email protected], www.jacksonimmuno.com
www.jireurope.com

Product Specifications

Product: Biotin-SP-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Human IgG, FcFragment Specific (minimal cross-reaction
to Bovine, Horse, and Mouse Serum Proteins)

Code Number: 709-066-098

Lot Number: 143214

Physical State: Freeze-dried solid

Size: 0.5 ml

Antibody Concentration: 1.0 mg/ml

Suggested Dilution Range: 1:20,000 - 1:400,000 for ELISA and Western blotting using enzyme-conjugated streptavidin
1:500 - 1:5,000 for enzyme immunohisto/cytochemistry
1:200 - 1:1,000 for flow cytometry and fluorescence immunohisto/cytochemistry

Biotin-SP: d-Biotinyl- -aminocaproic acid-NHS ester

Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6

Stabilizer: 15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free)

Preservative: 0.05% Sodium Azide

Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with 0.5 ml dH2O and centrifuge if not clear. Prepare working
dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.

Extended Storage after Rehydration: Aliquot and freeze at -70°C or below. Avoid repeated freezing and thawing. Alternatively, add
an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid. Expiration date:
one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.

Purity: The antibody was purified from antisera by a combination of pepsin digestion and immunoaffinity chromatography using
antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed.

Antibody Specificity: Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the Fc portion of human IgG heavy
chain but not with the Fab portion of human IgG. No antibody was detected against human IgM or IgA, or against
non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal
cross-reaction with bovine, horse, and mouse serum proteins, but it may cross-react with immunoglobulins from other species.

Country of Origin: USA

Note: For in vitro research use only, not for diagnostic or therapeutic use. This product is not a medical device.

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