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    Microfluidic Plasmonic Bio-Sensing of Exosomes by Using A Gold Nano-Island Platform

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    Durai Chelvan
    This document summarizes a research article that describes a new biosensing method for detecting exosomes using gold nano-islands and their plasmonic properties. The method adsorbs various molecules to the gold nanoparticles, shifting the plasmon band in a concentration-dependent manner. Exosomes are detected by binding them to a polypeptide via heat shock proteins. Optimization of linker molecules was needed to efficiently capture exosomes. The method was then transferred to a microfluidic device for sensing experiments, showing the plasmon shift corresponds to exosome concentration. This new approach could allow accurate exosome quantification and discrimination in clinical applications.

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    Microfluidic Plasmonic Bio-Sensing of Exosomes by Using A Gold Nano-Island Platform

    Uploaded by

    Durai Chelvan
    26 views1 page
    This document summarizes a research article that describes a new biosensing method for detecting exosomes using gold nano-islands and their plasmonic properties. The method adsorbs various molecules to the gold nanoparticles, shifting the plasmon band in a concentration-dependent manner. Exosomes are detected by binding them to a polypeptide via heat shock proteins. Optimization of linker molecules was needed to efficiently capture exosomes. The method was then transferred to a microfluidic device for sensing experiments, showing the plasmon shift corresponds to exosome concentration. This new approach could allow accurate exosome quantification and discrimination in clinical applications.

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    18CA050145

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    This document summarizes a research article that describes a new biosensing method for detecting exosomes using gold nano-islands and their plasmonic properties. The method adsorbs various molecules to the gold nanoparticles, shifting the plasmon band in a concentration-dependent manner. Exosomes are detected by binding them to a polypeptide via heat shock proteins. Optimization of linker molecules was needed to efficiently capture exosomes. The method was then transferred to a microfluidic device for sensing experiments, showing the plasmon shift corresponds to exosome concentration. This new approach could allow accurate exosome quantification and discrimination in clinical applications.

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
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    26 views1 page

    Microfluidic Plasmonic Bio-Sensing of Exosomes by Using A Gold Nano-Island Platform

    Uploaded by

    Durai Chelvan
    This document summarizes a research article that describes a new biosensing method for detecting exosomes using gold nano-islands and their plasmonic properties. The method adsorbs various molecules to the gold nanoparticles, shifting the plasmon band in a concentration-dependent manner. Exosomes are detected by binding them to a polypeptide via heat shock proteins. Optimization of linker molecules was needed to efficiently capture exosomes. The method was then transferred to a microfluidic device for sensing experiments, showing the plasmon shift corresponds to exosome concentration. This new approach could allow accurate exosome quantification and discrimination in clinical applications.

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    © All Rights Reserved
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    of 1

    World Academy of Science, Engineering and Technology

    International Journal of Mechanical and Mechatronics Engineering


    Vol:12, No:5, 2018

    Microfluidic Plasmonic Bio-Sensing of Exosomes by Using a Gold Nano-


    Island Platform
    Authors : Srinivas Bathini, Duraichelvan Raju, Simona Badilescu, Muthukumaran Packirisamy
    Abstract : A bio-sensing method, based on the plasmonic property of gold nano-islands, has been developed for detection of
    exosomes in a clinical setting. The position of the gold plasmon band in the UV-Visible spectrum depends on the size and shape
    Open Science Index, Mechanical and Mechatronics Engineering Vol:12, No:5, 2018 waset.org/abstracts/83124

    of gold nanoparticles as well as on the surrounding environment. By adsorbing various chemical entities, or binding them, the
    gold plasmon band will shift toward longer wavelengths and the shift is proportional to the concentration. Exosomes transport
    cargoes of molecules and genetic materials to proximal and distal cells. Presently, the standard method for their isolation and
    quantification from body fluids is by ultracentrifugation, not a practical method to be implemented in a clinical setting. Thus, a
    versatile and cutting-edge platform is required to selectively detect and isolate exosomes for further analysis at clinical level.
    The new sensing protocol, instead of antibodies, makes use of a specially synthesized polypeptide (Vn96), to capture and
    quantify the exosomes from different media, by binding the heat shock proteins from exosomes. The protocol has been
    established and optimized by using a glass substrate, in order to facilitate the next stage, namely the transfer of the protocol to
    a microfluidic environment. After each step of the protocol, the UV-Vis spectrum was recorded and the position of gold
    Localized Surface Plasmon Resonance (LSPR) band was measured. The sensing process was modelled, taking into account the
    characteristics of the nano-island structure, prepared by thermal convection and annealing. The optimal molar ratios of the
    most important chemical entities, involved in the detection of exosomes were calculated as well. Indeed, it was found that the
    results of the sensing process depend on the two major steps: the molar ratios of streptavidin to biotin-PEG-Vn96 and, the final
    step, the capture of exosomes by the biotin-PEG-Vn96 complex. The microfluidic device designed for sensing of exosomes
    consists of a glass substrate, sealed by a PDMS layer that contains the channel and a collecting chamber. In the device, the
    solutions of linker, cross-linker, etc., are pumped over the gold nano-islands and an Ocean Optics spectrometer is used to
    measure the position of the Au plasmon band at each step of the sensing. The experiments have shown that the shift of the Au
    LSPR band is proportional to the concentration of exosomes and, thereby, exosomes can be accurately quantified. An important
    advantage of the method is the ability to discriminate between exosomes having different origins.
    Keywords : exosomes, gold nano-islands, microfluidics, plasmonic biosensing
    Conference Title : ICME 2018 : International Conference on Mechanical Engineering
    Conference Location : Montreal, Canada
    Conference Dates : May 24-25, 2018

    International Scholarly and Scientific Research & Innovation 12(5) 2018 1 ISNI:0000000091950263

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