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Immunoregulatory Activities of Polysaccharides From Mung Bean

The document discusses several studies that investigated polysaccharides extracted from mung bean seeds and hulls. The studies found that the polysaccharides have antioxidant and immunoregulatory properties. Specifically, they were found to stimulate macrophage activity and secretion of molecules like NO, TNF-α, and IL-6. The polysaccharides also demonstrated significant antioxidant effects through free radical scavenging in a dose-dependent manner. Molecular weights and monosaccharide compositions of the extracted polysaccharides were analyzed in the studies.

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45 views

Immunoregulatory Activities of Polysaccharides From Mung Bean

The document discusses several studies that investigated polysaccharides extracted from mung bean seeds and hulls. The studies found that the polysaccharides have antioxidant and immunoregulatory properties. Specifically, they were found to stimulate macrophage activity and secretion of molecules like NO, TNF-α, and IL-6. The polysaccharides also demonstrated significant antioxidant effects through free radical scavenging in a dose-dependent manner. Molecular weights and monosaccharide compositions of the extracted polysaccharides were analyzed in the studies.

Uploaded by

Marjo
Copyright
© © All Rights Reserved
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
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Immunoregulatory activities of

polysaccharides from mung bean.
https://pubmed.ncbi.nlm.nih.gov/26794947/

Science.gov (United States)

Yao, Yang; Zhu, Yingying; Ren, Guixing

2016-03-30

Ultrasonic treatment was conducted on water-extractable mung bean seed


polysaccharides. MWP-1 'and MWP-2' were obtained by purified anion-exchange
and gel filtration chromatography. The MWP-1 'and MWP-2' average molecular
weights (Mws) were 68.4 kDa and 52.4 kDa, respectively. The analysis of
monosaccharides components showed that MWP-1 'composed of 0.4:2.6:5.3:0.7
molar percent of Rha, Ara, Man and Gal. MWP-2 was composed of
0.5:1.4:2.1:0.4 molar percent of Ara, Guy, Gal and Glc. The in vitro study showed
that both polysaccharide samples were capable of stimulating the development
of RAW264.7 murine macrophage secretory molecules (NO, TNF-α and IL-6) in a
dosage dependent manner. The NO production seemed to be the most potent
and significantly higher mediated by MWP-2. These results indicate that the
polysaccharides isolated in our study by ultrasonic treatment have immune
potentiation effects on macrophages.

Study on the preparation of


polysaccharides from mung bean seed
and anti-oxidant activity
https://www.researchgate.net/publication/289217039_Study_on_the_preparation_of_polysaccharides
_from_mung_bean_seed_and_anti-oxidant_activity

Response surface methodology (RSM) was used to optimize the effects of


processing parameters of alkali extraction on the yield of polysaccharides, and on
the basis of which, anti-oxidative activity was investigated. The optimal extraction
condition of polysaccharides was determined as follows with Alkali extraction
mung bean polysaccharides (AEMP): concentration of alkali solution was 0.02
mol/L, ratio of water to raw material was 20:1 mL/g, extraction temperature was
50°C and treatment time was 3.0 h. Under this condition, the experimental
recovery of crude alkali extraction mung bean polysaccharides (AEMP) was 9.70
mg GE/g DW. AEMP was purified by sephadex G-100 gel chromatogarph after
anion-exchange DEAE-52 cellulose chromatograph. A neutral fraction (AEMP-1)
and one acid fraction (AEMP-2) were gained. The result of anti-oxidative activity
showed that AEMP and its purified components showed excellent scavenging
activity on the hydroxyl radicals and a, a-diphenyl-1-picryl-hydrazyl (DPPH)
radicals. AEMP-2 had best radicals scavenging activity, and IC50 values was 4.71
mg/mL (hydroxyl radicals) and 1.03 × 10-1 mg/mL (DPPH), respectively.
Therefore, mung bean polysaccharides have a great anti-Oxidant activity.

Antioxidant activities of water-soluble


polysaccharide extracted from mung bean
(Vigna radiata L.) hull with ultrasonic
assisted treatment
https://www.researchgate.net/publication/223557693_Antioxidant_activities_of_water-
soluble_polysaccharide_extracted_from_mung_bean_Vigna_radiata_L_hull_with_ultrasonic_assisted_tre
atment

Ultrasonic assisted extraction and antioxidant activity of water-soluble


polysaccharide (MSP) from mung bean (Vigna radiata L.) hull were
investigated. After deproteinization, MSP was isolated and purified by a DEAE-
cellulose anion-exchange column and a Sephadex G-100 gel-permeation
column. Two acid polysaccharide fractions, MP1 and MP2, were characterized
by FT-IR, GC, and gel-permeation chromatography (GPC). Results indicated that
MP1 and MP2 were acid heteropolysaccharide with 9.9% and 36.4% of uronic
acid contents respectively. The main composition of MP1 was mannose,
whereas MP2 consisted of rhamnose and galactose. The molecule weights of
MP1 and MP2 were 83 kDa and 45 kDa, respectively. Although, MP2 showed
higher hydroxyl radical-scavenging activity, MP1 exhibited higher reducing
power and stronger scavenging capacity for superoxide radical and DPPH
radicals as well as higher inhibition on self-oxidation of 1,2,3-phentriol than
that of MP2. Thus, MP1 should be explored as a potential antioxidant.
Antioxidant and immunoregulatory
activity of alkali-extractable
polysaccharides from mung bean
https://pubmed.ncbi.nlm.nih.gov/26712695/

Alkali-extractable polysaccharides from the seeds of mung beans and two


polysaccharide sub-fractions (MAP-1 and MAP-2) were isolated and purified by
anion-exchange and gel filtration chromatography. The average molecular
weights (Mws) of MAP-1 and MAP-2 were 94.2 kDa and 60.4 kDa, respectively.
Monosaccharide component analysis indicated that MAP-1 was composed of Rha,
Ara, Glu, Gal, and GalA in a molar ratio of 1.1:0.4:0.7:0.5:0.3. MAP-2 consisted of
Xyl, Rha, Gal, Glu and GalA with a relative molar ratio of 0.4:1.4:1.6:0.5:0.2.
Antioxidant assays indicated that both MAP-1 and MAP-2 exhibit significant
antioxidant activity in a dose-dependent manner. An in vitro study further
showed that MAP-1 and MAP-2 were both able to stimulate the production of
secretory molecules (NO, TNF-α and IL-6) by RAW 264.7 murine macrophages in a
concentration-dependent manner. These findings suggest that the
polysaccharides isolated in our study have immunoregulatory effects on
macrophages and can be used as a beneficial health food.

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