Spectrophotometric Determination of Adefovir Dipiv
Spectrophotometric Determination of Adefovir Dipiv
Spectrophotometric Determination of Adefovir Dipiv
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Y N Manohara
Jazan University
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Introduction
Adefovir dipivoxil is an antiviral drug and chemically [ 2-(6-aminopurine–9yl) ethoxy methyl-
(2,2-dimethyl propanoyloxy methoxy phosphoryl) oxy methyl 2,2-dimethyl propanoate1,2.
Adefovir dipivoxil is a prodrug of adefovir. Adefovir is an acyclic nucleotide analong of
adenosine monophosophate3. It is a new generation antiviral drug which is active in-vitro
against HBV. It is indicated for the treatment of chronic hepatitis-B and HIV. It is not official in
any pharmacopoeia. Literature survey reveals that no visible methods are reported however a
few bio-analytical methods were reported using human plasma by HPLC4 & LCMS/MS5.
Spectrophotometric Determination of Adefovir Dipivoxil 714
NH2
R NH 2
N N
N N O O
O P O O CH 3
O CH 3
H3 C
O CH 3
O CH 3
CH 3
Experimental
Shimadzu model 1700 double beam UV-visible spectrophotometer with a pair of 1 cm
matched quartz cells was used to measure absorbance of the resulting solutions. All the
chemicals used were of AR grade procured from qualigens Mumbai.
Preparation of reagents
3- Methyl –2- benzo thiazolinone hydrazones (0.2 %)
200 mg of MBTH was dissolved in 100 mL of distilled water.
Ferric chloride (0.2 %)
Ferric chloride 0.2% was freshly prepared by dissolving 200 mg of ferric chloride in 100 mL
of distilled water.
Folins reagent (sodium 1,2-naphthaquinone –4- sulphonate ) (1 %w/v)
Folins reagent was prepared by dissolving 1 g of sodium 1, 2-naphthaquinone-4- sulphonate
in 100 mL of distilled water.
Sodium hydroxide (5 %w/v)
Sodium hydroxide solution was prepared by dissolving 5 g in 100 mL of distilled water.
Method A
Preparation of sample solutions
Twenty tablets were powdered and an amount equivalent to 100 mg of drug was dissolved in
50 mL of distilled water, 9.0 mL of concentrated HCl was added, refluxed for 2 h and the
solution was diluted to 100 mL with distilled water to obtain 1 mg/mL solution. The
hydrolyzed drug solution was filtered and further diluted with distilled water to obtain a
concentration of 100 µg/mL.
715 Y. N. MANOHARA et al.
Conclusions
Commercial formulation of adefovir dipivoxil tablet was successfully analyzed by the
proposed method. The accuracy and validity of the proposed methods were further
ascertained by performing recovery studies. Pre-analyzed sample was spiked with pure drug
and the total quantity was found by the proposed methods. The recovery of the pure drug
added was quantitative and revealed that there is no interference of excipients in the
determination. The results of recovery study were shown in Table 2. In conclusion the
proposed spectrophotometric method for the estimation of adefovir dipivoxil is simple,
sensitive, and accurate and can be used for the routine quality control of the drug in bulk as
well as in pharmaceutical formulations.
Acknowledgements
The authors are grateful to the Head, Department of Pharmaceutical Analysis for providing
research facilities, authors are also thankful to the National Education Society, Shimoga.
References
1. O' Neil MJ. The Merck Index – An encyclopedia of chemicals, drugs and biologicals,
13th ed. New Jersy: Merk & Co., INC, 2001.
717 Y.N.MANOHARA et al.