Centrifugation Seperate of Blood

Download as docx, pdf, or txt
Download as docx, pdf, or txt
You are on page 1of 1

Objectives.

Upon completion of this experiment,students should be able:


a. To separate the red blood cells from the plasma.

Introduction.

Blood, fluid that transports oxygen and nutrients to the cells and carries away carbon
dioxide and other waste products. Technically, blood is a transport liquid pumped by the
heart (or an equivalent structure) to all parts of the body, after which it is returned to the heart
to repeat the process.Blood consists of two types of cells: Red Blood Cells (RBC), called also
erythrocytes and White Blood Cells (WBC), called leucocytes. Erythrocytes are the blood
cells that distribute oxygen from the lungs to all the other parts throughout the whole
organism, whereas the leucocytes are responsible for attacking any infectious cells, sustaining
the optimal performance of the whole immune system.White Blood Cells can be furthermore
categorized into: lymphocytes, monocytes, eosinophils, basophils, and neutrophils
(granulocytes).
Centrifugation is the process of separating substance of different densities from a mixture
with the help of centrifuge.The use of centrifuge technology is critical in the clinical
laboratory setting for the separation of liquid and solid components. In laboratories
performing biochemical analyses on body fluids, centrifuges are routinely used to separate
blood cells from serum/plasma, to separate sediment from urine, to measure the volume
fraction of erythrocytes in blood (the hematocrit), and to separate bound from free
components in protein binding and immunoprocedures. Less routinely, centrifugation is used
for separation of lipoproteins in reference procedures for their measurement, separation of
cellular components, and separation of DNA fragments.

You might also like