Structure and Function of RNA

3/22/2020 Structure and Function of RNA - Microbiology - OpenStax
LEARNING OBJECTIVES
Describe the biochemical structure of ribonucleotides

Describe the similarities and differences between RNA and DNA
Describe the functions of the three main types of RNA used in protein synthesis
Explain how RNA can serve as hereditary information
Structurally speaking, ribonucleic acid (RNA), is quite similar to DNA. However, whereas DNA
molecules are typically long and double stranded, RNA molecules are much shorter and are typically
single stranded. RNA molecules perform a variety of roles in the cell but are mainly involved in the
process of protein synthesis (translation) and its regulation.
RNA Structure
RNA is typically single stranded and is made of ribonucleotides that are linked by phosphodiester
bonds. A ribonucleotide in the RNA chain contains ribose (the pentose sugar), one of the four
nitrogenous bases (A, U, G, and C), and a phosphate group. The subtle structural difference between
the sugars gives DNA added stability, making DNA more suitable for storage of genetic information,
whereas the relative instability of RNA makes it more suitable for its more short-term functions. The
RNA-specific pyrimidine uracil forms a complementary base pair with adenine and is used instead of
the thymine used in DNA. Even though RNA is single stranded, most types of RNA molecules show
extensive intramolecular base pairing between complementary sequences within the RNA strand,
creating a predictable three-dimensional structure essential for their function (Figure 10.20 and Figure
10.21).
Figure 10.20 (a) Ribonucleotides contain the pentose sugar ribose instead of the deoxyribose found in
deoxyribonucleotides. (b) RNA contains the pyrimidine uracil in place of thymine found in DNA.
https://openstax.org/books/microbiology/pages/10-3-structure-and-function-of-rna 1/5
Figure 10.21 (a) DNA is typically double stranded, whereas RNA is typically single stranded. (b) Although it is single
stranded, RNA can fold upon itself, with the folds stabilized by short areas of complementary base pairing within the
molecule, forming a three-dimensional structure.
CHECK YOUR UNDERSTANDING
How does the structure of RNA differ from the structure of DNA?
Functions of RNA in Protein Synthesis

Cells access the information stored in DNA by creating RNA to direct the synthesis of proteins through
the process of translation. Proteins within a cell have many functions, including building cellular
structures and serving as enzyme catalysts for cellular chemical reactions that give cells their specific
characteristics. The three main types of RNA directly involved in protein synthesis are messenger RNA
(mRNA), ribosomal RNA (rRNA), and transfer RNA (tRNA).
In 1961, French scientists François Jacob and Jacques Monod hypothesized the existence of an
intermediary between DNA and its protein products, which they called messenger RNA.16 Evidence
supporting their hypothesis was gathered soon afterwards showing that information from DNA is
transmitted to the ribosome for protein synthesis using mRNA. If DNA serves as the complete library of
cellular information, mRNA serves as a photocopy of specific information needed at a particular point in
time that serves as the instructions to make a protein.
The mRNA carries the message from the DNA, which controls all of the cellular activities in a cell. If a
cell requires a certain protein to be synthesized, the gene for this product is “turned on” and the mRNA
is synthesized through the process of transcription (see RNA Transcription). The mRNA then interacts
with ribosomes and other cellular machinery (Figure 10.22) to direct the synthesis of the protein it
encodes during the process of translation (see Protein Synthesis). mRNA is relatively unstable and short-
lived in the cell, especially in prokaryotic cells, ensuring that proteins are only made when needed.
Figure 10.22 A generalized illustration of how mRNA and tRNA are used in protein synthesis within a cell.
rRNA and tRNA are stable types of RNA. In prokaryotes and eukaryotes, tRNA and rRNA are encoded in
the DNA, then copied into long RNA molecules that are cut to release smaller fragments containing the
individual mature RNA species. In eukaryotes, synthesis, cutting, and assembly of rRNA into ribosomes
takes place in the nucleolus region of the nucleus, but these activities occur in the cytoplasm of
prokaryotes. Neither of these types of RNA carries instructions to direct the synthesis of a polypeptide,
but they play other important roles in protein synthesis.
Ribosomes are composed of rRNA and protein. As its name suggests, rRNA is a major constituent of
ribosomes, composing up to about 60% of the ribosome by mass and providing the location where the
mRNA binds. The rRNA ensures the proper alignment of the mRNA, tRNA, and the ribosomes; the rRNA
of the ribosome also has an enzymatic activity (peptidyl transferase) and catalyzes the formation of the
peptide bonds between two aligned amino acids during protein synthesis. Although rRNA had long been
thought to serve primarily a structural role, its catalytic role within the ribosome was proven in 2000.17
Scientists in the laboratories of Thomas Steitz (1940–) and Peter Moore (1939–) at Yale University were
able to crystallize the ribosome structure from Haloarcula marismortui, a halophilic archaeon isolated
from the Dead Sea. Because of the importance of this work, Steitz shared the 2009 Nobel Prize in
Chemistry with other scientists who made significant contributions to the understanding of ribosome
structure.
Transfer RNA is the third main type of RNA and one of the smallest, usually only 70–90 nucleotides long.
It carries the correct amino acid to the site of protein synthesis in the ribosome. It is the base pairing
between the tRNA and mRNA that allows for the correct amino acid to be inserted in the polypeptide
chain being synthesized (Figure 10.23). Any mutations in the tRNA or rRNA can result in global problems
for the cell because both are necessary for proper protein synthesis (Table 10.1).
Figure 10.23 A tRNA molecule is a single-stranded molecule that exhibits significant intracellular base pairing, giving it its
characteristic three-dimensional shape.
Structure and Function of RNA
mRNA rRNA tRNA
Structure Short, unstable, Longer, stable RNA Short (70-90 nucleotides),

single-stranded molecules composing 60% stable RNA with extensive
RNA corresponding of ribosome’s mass intramolecular base pairing;
to a gene encoded contains an amino acid
within DNA binding site and an mRNA
binding site
Function Serves as Ensures the proper Carries the correct amino

intermediary alignment of mRNA, tRNA, acid to the site of protein
between DNA and and ribosome during synthesis in the ribosome
protein; used by protein synthesis; catalyzes
ribosome to direct peptide bond formation
synthesis of protein between amino acids
it encodes
Table 10.1
CHECK YOUR UNDERSTANDING
What are the functions of the three major types of RNA molecules involved in protein
synthesis?
RNA as Hereditary Information

Although RNA does not serve as the hereditary information in most cells, RNA does hold this function
for many viruses that do not contain DNA. Thus, RNA clearly does have the additional capacity to serve
as genetic information. Although RNA is typically single stranded within cells, there is significant
diversity in viruses. Rhinoviruses, which cause the common cold; influenza viruses; and the Ebola virus
are single-stranded RNA viruses. Rotaviruses, which cause severe gastroenteritis in children and other
immunocompromised individuals, are examples of double-stranded RNA viruses. Because double-
stranded RNA is uncommon in eukaryotic cells, its presence serves as an indicator of viral infection. The
implications for a virus having an RNA genome instead of a DNA genome are discussed in more detail in
Viruses.
Footnotes
16 A. Rich. “The Era of RNA Awakening: Structural Biology of RNA in the Early Years.” Quarterly
Reviews of Biophysics 42 no. 2 (2009):117–137.
17 P. Nissen et al. “The Structural Basis of Ribosome Activity in Peptide Bond Synthesis.” Science
289 no. 5481 (2000):920–930.

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3/22/2020 Structure and Function of RNA - Microbiology - OpenStax

Describe the biochemical structure of ribonucleotides

CHECK YOUR UNDERSTANDING

Functions of RNA in Protein Synthesis

mRNA rRNA tRNA

Structure Short, unstable, Longer, stable RNA Short (70-90 nucleotides),

Function Serves as Ensures the proper Carries the correct amino

CHECK YOUR UNDERSTANDING

RNA as Hereditary Information

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