Nitrate Determination in Water Post Laboratory Report: Figure 1. Shows The Results in Tabulated Form

Llaguno, John Luel R.
Magtaca, Michaela Abigail C.
Nebres, Meinard Nogui C.
Zamora, Jaymel
BS-CHEMISTRY 2B
NITRATE DETERMINATION IN WATER

Laboratory Activity No.2
Post Laboratory Report
I. RESULTS AND DISCUSSION
The virtual experiment conducted a technique that was used to measure light absorbance across
the ultraviolet and visible ranges of the electromagnetic spectrum using a UV-Vis spectrophotometer.
Photometric mode measures absorbance and transmittance at a single wavelength or at multiple
wavelengths, it is the method that was used in this virtual laboratory experiment. The concentration levels
of the field samples in the unit of ppm (parts per million) were measured using standard calibration.
Sample 1 is observed to be at 1.590 ppm, field sample 2 was measured 1.117 ppm and lastly for the field
sample 3 with the concentration of 1.578 ppm.
Figure 1. Shows the results in tabulated form

Sample/Unknown Concentration (ppm)
Field sample 1 1.590
This virtual laboratory experiment gives knowledge in familiarizing the work flow of the UV-Vis
spectrophotometer and its uses, particularly its aid to humanity as it measures concentrations of different
chemicals as well as curves of bacterial cell suspensions that helps us determine what is safe or what
substance is harmful.
A spectrometer is a piece of a spectrophotometer which is responsible for the measuring of

various items, while a spectrophotometer is the complete system that includes a light source; a means to
collect the light that has interacted with the tested items; and a spectrometer for measurements. The UV-
Vis spectrophotometer is commonly used for analysis of transition metal ion, highly conjugated organic
compounds, and biological macromolecules. When using UV-Vis spectrophotometers they should be
installed in an air-conditioned room with humidity of 85% and below. It is necessary to consider the
humidity level in the working area while dealing with a spectrophotometer because water molecules
generally absorb UV light, thus if humidity increases, there is a decrease in absorbance. For that we may
require more UV lamps. Decrease in humidity should result in decreasing in transmission. As we required
absorbance value of a sample, thus maximum absorbance gives a neat value, if humidity increase in the
room, then water particles present in sample may decrease the absorbance. Also humidity has an effect
on detector of UV instrument. the detector is a photomultiplier tube, which contains a photosensitive
cathode, anode and several dynodes. Most of these are made up of silicon photodiodes. Thus, increase in
humidity creates more water particles which can damage the detector.
Cuvettes have many types, but the three most common types are plastic cuvette, glass Cuvettes,
and quartz cuvette. Plastic Cuvettes are often used in fast spectroscopic assays, where high speed is more
important than high accuracy. The glass one has an optimal wavelength range of 340-2500 nm and are
typically for use in wavelength range of visible light. The last one, which is the quartz cuvette provide more
durability than plastic and glass and it excels at transmitting UV light, and can be used for wavelength
ranging from 190-2500 nm. Proper handling and proper maintenance of cuvettes is crucial and important
in order to obtain a more accurate and reliable results in a spectrophotometer. When holding cuvettes
properly, where its transparent sides are not being touched and its opaque sides are held between two
fingers. Proper cuvette cleaning is necessary also. Also, light scattering may occur if there are scratches
present in a cuvette, which will also affect measurements that can be obtained. When filling the cuvettes,
avoid spilling the solution on the outer side of the cuvettes.
II. CONCLUSION
To conclude the experiment, the analyst was able to obtain the results through the analysis of
formaldehyde in water using UV-Vis spectrophotometer. It was observed that the field sample 1 has 1.590
ppm of nitrate in water while in field sample 2 there was a 1.117 ppm of nitrate collected, and in field
sample 3, 1.578 ppm of nitrate was collected.
The purpose of familiarizing the common workflow in a laboratory analysis involving the used of
UV-Vis spectrophotometer was attained. Due to some discrepancies in the alternative experiment with
the original experiment, the hypothesis which states that if Brucine method will give consistent and
reproducible results, then it can be used as a reliable method in determining the nitrates in water was not
tested.
III. REFERENCES
Mettler-Toledo International Inc. all rights reserved. (2020, February 24). Life science toolbox for
Uv/vis spectroscopy. Retrieved March 29, 2021, from
https://www.mt.com/ph/en/home/library/guides/laboratory-division/life-science/uvvis-
toolbox-for-life-
sciences.html#:~:text=The%20most%20common%20applications%20are,curves%20of%20b
acterial%20cell%20suspensions.
Stubbings, J. (n.d.). Uv-visible spectroscopy tutorial. Retrieved March 29, 2021, from
https://www.ausetute.com.au/uvvisible.html
UV-VIS spectrophotometer. (n.d.). Retrieved March 29, 2021, from

https://www.holmarc.com/UV_VIS_spectrophotometer.php
What is A Uv-vis spectrophotometer? (2021, February 17). Retrieved March 29, 2021, from
https://www.denovix.com/blog/what-is-a-uv-vis-spectrophotometer/
IV. APPENDICES
APPENDIX A. GROUP CHAT DISCUSSIONS

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Llaguno, John Luel R.

Magtaca, Michaela Abigail C.

Nebres, Meinard Nogui C.

NITRATE DETERMINATION IN WATER

I. RESULTS AND DISCUSSION

Figure 1. Shows the results in tabulated form

A spectrometer is a piece of a spectrophotometer which is responsible for the measuring of

UV-VIS spectrophotometer. (n.d.). Retrieved March 29, 2021, from

APPENDIX A. GROUP CHAT DISCUSSIONS

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