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    Kurva Kalibrasi STD PCT: Analisis Data Pengujian Paracetamol Dengan HPLC

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    aprilia kurnia putri
    This document summarizes an HPLC analysis of paracetamol. It includes a calibration curve with R2 of 0.9936. Limit of detection was determined to be 0.0521 and limit of quantification was 0.1736. Three sample peaks were analyzed and two spike samples were used to determine recovery of 196.3297% and 199.4395%. Precision was determined to be 1.99% RSD for peak area and 3.48% RSD for samples. Tailing factor and asymmetry factors were all 1. The average concentration of samples was determined to be 1.9450% with an average peak area of 37027.8467.

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    Kurva Kalibrasi STD PCT: Analisis Data Pengujian Paracetamol Dengan HPLC

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    aprilia kurnia putri
    47 views4 pages
    This document summarizes an HPLC analysis of paracetamol. It includes a calibration curve with R2 of 0.9936. Limit of detection was determined to be 0.0521 and limit of quantification was 0.1736. Three sample peaks were analyzed and two spike samples were used to determine recovery of 196.3297% and 199.4395%. Precision was determined to be 1.99% RSD for peak area and 3.48% RSD for samples. Tailing factor and asymmetry factors were all 1. The average concentration of samples was determined to be 1.9450% with an average peak area of 37027.8467.

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    PERCOBAAN 4 HPLC

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    This document summarizes an HPLC analysis of paracetamol. It includes a calibration curve with R2 of 0.9936. Limit of detection was determined to be 0.0521 and limit of quantification was 0.1736. Three sample peaks were analyzed and two spike samples were used to determine recovery of 196.3297% and 199.4395%. Precision was determined to be 1.99% RSD for peak area and 3.48% RSD for samples. Tailing factor and asymmetry factors were all 1. The average concentration of samples was determined to be 1.9450% with an average peak area of 37027.8467.

    Copyright:

    © All Rights Reserved
    Download as XLSX, PDF, TXT or read online from Scribd
    Download as xlsx, pdf, or txt
    47 views4 pages

    Kurva Kalibrasi STD PCT: Analisis Data Pengujian Paracetamol Dengan HPLC

    Uploaded by

    aprilia kurnia putri
    This document summarizes an HPLC analysis of paracetamol. It includes a calibration curve with R2 of 0.9936. Limit of detection was determined to be 0.0521 and limit of quantification was 0.1736. Three sample peaks were analyzed and two spike samples were used to determine recovery of 196.3297% and 199.4395%. Precision was determined to be 1.99% RSD for peak area and 3.48% RSD for samples. Tailing factor and asymmetry factors were all 1. The average concentration of samples was determined to be 1.9450% with an average peak area of 37027.8467.

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    © All Rights Reserved
    Download as XLSX, PDF, TXT or read online from Scribd
    Download as xlsx, pdf, or txt
    of 4

    ANALISIS DATA PENGUJIAN PARACETAMOL DENGAN HPLC

    1. Penentuan Kurva Kalibrasi


    Konsentrasi (%) Luas Area Height Width 0,1 Width 0,05 Width
    0.1 21089.79 3488.9448 0.092 0.0092 0.0046
    0.3 32952.52 3760.1357 0.135 0.0135 0.00675
    0.4 37120.67 3797.4243 0.153 0.0153 0.00765

    Kurva Kalibrasi Std PCT


    40000
    35000 f(x) = 54275.8928571428 x + 15914.0885714286
    R² = 0.993580153070451
    30000
    25000
    Luas Area

    20000
    15000
    10000
    5000
    0
    0.05 0.1 0.15 0.2 0.25 0.3 0.35 0.4 0.45
    Konsentrasi

    Slope 54276
    Intersep 15914
    R2 0.9936
    r 0.9968

    2. Penentuan LOD dan LOQ


    Konsentrasi (%) y yi (y-yi)2
    0.1 21089.79 21341.6 63408.28
    0.3 32952.52 32196.8 571112.7
    0.4 37120.67 37624.4 253743.9
    Σ 888264.9
    sy/x 942.4781
    LOD 0.0521
    LOQ 0.1736

    3. Penentuan Sampel
    Nama Sampel Luas Area Height width 0,1 Width 0,05 Width

    Sampel 1 36806.29 3786.51 0.152 0.0152 0.0076


    Sampel 2 36428.29 3791.76 0.150 0.0150 0.0075
    Sampel 3 37848.96 3764.43 0.159 0.0159 0.0080
    Spike 1 45552.64 3801.59 0.192 0.0192 0.0096
    Spike 2 45687.67 3808.55 0.193 0.0193 0.00965
    SD Sampel 0.0678
    SD Luas Area 735.7930

    4. Penentuan Recovery
    Keterangan Kadar (%) 0.1
    spike 1 2.7304 0.3
    Spike 2 2.7428 0.5
    Recovery 1 196.3297
    Recovery 2 199.4395
    C target 1 0.4
    C target 2 1
    60000
    5. Penentuan Presisi
    50000 f(x) = 84577.7 x + 122
    Keterangan Kadar % R² = 0.9988228619855
    RSD sampel 3.48
    40000
    log C -1.71
    CV Horwitz 2.86 30000
    RSD Luas Area 1.99
    20000

    10000

    0
    0.05 0.1 0.15 0.2

    slope
    Tailing Factor Factor Asimetris
    1 1
    1 1
    1 1

    Rata-rata Kadar Rata-Rata Luas Asimetris


    Kadar (%) Tailing Factor
    sampel area sampel Factor
    1.9246 1 1
    1.8898 1 1
    2.0207 1.9450 37027.8467 1 1
    2.7304 1 1
    1.9450 37027.8467

    2.7428 1 1

    21089.79
    36999.52
    54920.87

    Chart Title
    000

    000 f(x) = 84577.7 x + 12296.75


    R² = 0.998822861985563

    000

    000

    000

    000

    0
    0.05 0.1 0.15 0.2 0.25 0.3 0.35 0.4 0.45 0.5 0.55

    84578 0.29
    12297 9.6594

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