Scribd Logo
Upload

Welcome to Scribd!

  • 34 views

    Molecular Biology: ICMR Registration No: RPPLPMP

    Uploaded by

    Maths Tricks solution Mr. G C
    Mr. Raghu Sharma, a 31-year-old male, tested positive for SARS-CoV-2 RNA via a nasopharyngeal swab sample. The test detected both the E gene and RdRp and N genes of SARS-CoV-2, with a Ct value of 19.29. This confirms the presence of an active COVID-19 infection in the sample. The test was performed using an ICMR-approved kit at an accredited laboratory.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd

    Molecular Biology: ICMR Registration No: RPPLPMP

    Uploaded by

    Maths Tricks solution Mr. G C
    34 views2 pages
    Mr. Raghu Sharma, a 31-year-old male, tested positive for SARS-CoV-2 RNA via a nasopharyngeal swab sample. The test detected both the E gene and RdRp and N genes of SARS-CoV-2, with a Ct value of 19.29. This confirms the presence of an active COVID-19 infection in the sample. The test was performed using an ICMR-approved kit at an accredited laboratory.

    Original Title

    labreportnew (1)

    Copyright

    © © All Rights Reserved

    Available Formats

    PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    Mr. Raghu Sharma, a 31-year-old male, tested positive for SARS-CoV-2 RNA via a nasopharyngeal swab sample. The test detected both the E gene and RdRp and N genes of SARS-CoV-2, with a Ct value of 19.29. This confirms the presence of an active COVID-19 infection in the sample. The test was performed using an ICMR-approved kit at an accredited laboratory.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    34 views2 pages

    Molecular Biology: ICMR Registration No: RPPLPMP

    Uploaded by

    Maths Tricks solution Mr. G C
    Mr. Raghu Sharma, a 31-year-old male, tested positive for SARS-CoV-2 RNA via a nasopharyngeal swab sample. The test detected both the E gene and RdRp and N genes of SARS-CoV-2, with a Ct value of 19.29. This confirms the presence of an active COVID-19 infection in the sample. The test was performed using an ICMR-approved kit at an accredited laboratory.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 2

    NAME :Mr.

    RAGHU SHARMA
    Age/Gender :31 Y/Male Requisition Date :03/May/2021 07:11PM
    Test Request ID :0062105030064 Sample CollDate :03/May/2021 09:33PM
    Bar Code No :11607041 Sample Rec.Date :04/May/2021 12:18AM
    Referred Doctor : Reporting Date :04/May/2021 08:52PM
    Panel Name :PC 3002 (BROOKLYN HIMALAYA)

    MOLECULAR BIOLOGY
    COVID-19 RT PCR (QUALITATIVE)
    ICMR Registration No: RPPLPMP

    The test has been performed at (Polo Labs Pvt. Ltd., IVY Hospital, Sector 71, Mohali).

    Method: RT PCR
    Specimen type: Nasopharyngeal swab

    Targets Observation
    E Gene (B-βCoV) Detected
    RdRpGene and N gene (SARS-CoV-2) Detected
    Ct Value (RdRpGene and N gene) 19.29
    Conclusion Sample is POSITIVE for SARS-CoV-2 RNA
    Remarks

    Clinical utility of the test


    Coronaviruses (CoV) are a large family of viruses that cause illness ranging from the common cold to more severe diseases such as Middle East
    Respiratory Syndrome (MERS-CoV) and Severe Acute Respiratory Syndrome (SARS-CoV). SARS-CoV-2, is the causative agent of the coronavirus disease
    2019 (COVID-19). Common signs of infection include respiratory symptoms, fever, cough, shortness of breath and breathing difficulties. In more severe cases,
    infection can cause pneumonia, severe acute respiratory syndrome and organ failure. The SARS-CoV-2 RNA is generally detectable in nasopharyngeal /
    oropharyngeal swabs during the acute phase of infection. Positive results are indicative of active infection. This assay is based on real-time PCR technology, for
    the qualitative detection of COVID-19 Viral RNA. This Real Time PCR assay targets both the E-gene (B-βCoV) which is recommended as a screening gene to
    detect the presence of lineage B-betacoronovirus and RdRp/N gene (SARS-CoV-2), which confirms presence of infection with COVID-19.

    Note: This test is performed using ICMR approved kit.

    Interpretation:
    Sr.No. Result Comment
    Both E gene AND RdRp and N gene Not Absence of E gene and SARS-CoV-2 RNA indicate no infection with
    1 Detected Sarbecovirus (B-βCoV).
    E gene Detected AND RdRp and N gene Presence of E gene indicates infection with Sarbecovirus (B-βCoV) BUT
    2 Not Detected absence of SARS-CoV-2

    Sr.No Result Comment

    The highlighted values should be correlated clinically

    Result Entered By:Simran 40631


    Page 1 of 2
    NAME :Mr. RAGHU SHARMA
    Age/Gender :31 Y/Male Requisition Date :03/May/2021 07:11PM
    Test Request ID :0062105030064 Sample CollDate :03/May/2021 09:33PM
    Bar Code No :11607041 Sample Rec.Date :04/May/2021 12:18AM
    Referred Doctor : Reporting Date :04/May/2021 08:52PM
    Panel Name :PC 3002 (BROOKLYN HIMALAYA)
    Both E gene AND RdRp and N gene
    3 Both E gene AND RdRp and N gene Detected
    Detected
    E gene Not Detected But RdRp and N Presence of SARS- CoV-2 (N or/and RdRp) specific RNA indicates
    4
    gene Detected infection with COVID-19.
    Inconclusive, RTPCR failure due to possible presence of Inhibitors.
    5 Internal Control Not Detected
    Repeat sample is requested

    Test Attributes and Limitations:

    1. Samples must be received at the laboratory under appropriate conditions within 48hrs of collection to ensure preservation of viral RNA.
    2. Negative result does not rule out the possibility of Covid -19 infection. Presence of inhibitors, mutations & insufficient organism RNA can
    influence the result. False Negative results may be seen in samples collected too early or too late in the clinical course of the infection. Kindly refer to the
    latest ICMR guidelines.
    3. A single negative test result, particularly if this is from an upper respiratory tract specimen, does not exclude infection. A positive alternate pathogen
    does not necessarily rule out COVID-19 infection, as little is yet known about the role of coinfections.
    4. Repeat sampling and testing of lower respiratory specimen is strongly recommended in severe or progressive disease. The repeat specimens may be
    considered after a gap of 2 - 4 days after the collection of the first specimen for additional testing if required.

    Patient Instructions:

    1. Kindly consult referring Physician/ Authorized Govt. hospital for appropriate follow up.
    2. Details of all the positive patients will be communicated to Epidemiology Cell whom you are requested to support.
    3. “Positive” status needs to be notified to the appropriate authorities as per the existing rules/regulations, while we shall
    also be doing the same.

    *** End Of Report ***

    The highlighted values should be correlated clinically

    Result Entered By:Simran 40631


    Page 2 of 2

    You might also like