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    Practical Report 1: FHSC1214 Fundamentals of Cell Biology Foundation in Science

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    This practical report summarizes an experiment identifying biomolecules using various food tests. Benedict's test identified glucose and lactose as reducing sugars that turned the solution blue, while sucrose and starch did not react. Sucrose was identified as a non-reducing sugar but hydrolyzed into reducing sugars with HCl. Starch turned the solution blue-black. Sudan III and an oil test identified coconut oil as a lipid. The Biuret test identified an unknown solution as containing protein when it turned from blue to violet.

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    Practical Report 1: FHSC1214 Fundamentals of Cell Biology Foundation in Science

    Uploaded by

    Eng
    83 views3 pages
    This practical report summarizes an experiment identifying biomolecules using various food tests. Benedict's test identified glucose and lactose as reducing sugars that turned the solution blue, while sucrose and starch did not react. Sucrose was identified as a non-reducing sugar but hydrolyzed into reducing sugars with HCl. Starch turned the solution blue-black. Sudan III and an oil test identified coconut oil as a lipid. The Biuret test identified an unknown solution as containing protein when it turned from blue to violet.

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    FHSC1214 Partial Report 1

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    This practical report summarizes an experiment identifying biomolecules using various food tests. Benedict's test identified glucose and lactose as reducing sugars that turned the solution blue, while sucrose and starch did not react. Sucrose was identified as a non-reducing sugar but hydrolyzed into reducing sugars with HCl. Starch turned the solution blue-black. Sudan III and an oil test identified coconut oil as a lipid. The Biuret test identified an unknown solution as containing protein when it turned from blue to violet.

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    © All Rights Reserved
    Download as DOC, PDF, TXT or read online from Scribd
    Download as doc, pdf, or txt
    83 views3 pages

    Practical Report 1: FHSC1214 Fundamentals of Cell Biology Foundation in Science

    Uploaded by

    Eng
    This practical report summarizes an experiment identifying biomolecules using various food tests. Benedict's test identified glucose and lactose as reducing sugars that turned the solution blue, while sucrose and starch did not react. Sucrose was identified as a non-reducing sugar but hydrolyzed into reducing sugars with HCl. Starch turned the solution blue-black. Sudan III and an oil test identified coconut oil as a lipid. The Biuret test identified an unknown solution as containing protein when it turned from blue to violet.

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    FHSC1214

    FUNDAMENTALS OF CELL BIOLOGY


    FOUNDATION IN SCIENCE

    Practical Report 1

    Name Eng Kher Xian


    ID Number 030421-01-0168
    Practical Group P5
    Experiment Title Lab 1: Biological Molecules I
    Date of
    Experiment
    Session (eg. 202105)
    Lecturer
    Experiment Title: Identification of Biomolecules Using Various Food Tests

    Objective: To identify the biomolecules present in a solution using various food tests
    (Benedict’s, Iodine, Sudan III, Emulsion & Biuret)

    Result:

    Test for reducing sugars:

    Test tube Carbohydrate Observation


    A Glucose Clear blue solution turns
    into brick red solution with
    brick red precipitate.
    B Lactose Clear blue solution turns
    into brick red solution with
    brick red precipitate.
    C Sucrose Clear blue solution remains
    unchanged.
    D Starch Clear blue solution remains
    unchanged.

    Conclusion: Glucose and lactose are reducing sugars. Sucrose and starch are non-
    reducing sugars.

    Test for non-reducing sugars:

    Carbohydrate Observation
    Sucrose (sugarcane) Clear blue solution turns into brick red
    solution with brick red precipitate. Sucrose
    is being hydrolysed into glucose and
    fructose by HCL.

    Conclusion: Sucrose is a non-reducing sugar. HCL hydrolysed sucrose into fructose and
    glucose that reacts with the Benedict’s solution to give brick red precipitate and solution.

    Test for starch:

    Observation Yellowish solution turns into blue-black.

    Conclusion: Potato is a starch.

    Test for lipid using Sudan III:

    Observation Before After


    Clear coconut oil. Pink droplets appear in the
    solution indicate the

    present of fat.

    Conclusion: Coconut oil

    Test for lipid using oil:

    Observation Before After


    Clear coconut oil.

    Test for protein:

    Observation Clear blue solution turns into violet

    solution.

    Conclusion:

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