MabSelect SuRe

Affinity chromatography

Instructions for Use

MabSelect SuRe™ is an alkali-tolerant protein A-derived BioProcess™ resin
for capturing monoclonal antibodies from large volumes of feed by packed
bed chromatography. BioProcess chromatography resins are developed
and supported for production-scale chromatography. BioProcess resins
are produced with validated methods and are tested to meet
manufacturing requirements. Secure ordering and delivery routines give a
reliable supply of resins for production-scale. Regulatory Support Files
(RSF) are available to assist process validation and submissions to
regulatory authorities. BioProcess resins cover all purification steps from
capture to polishing.
• Alkali-tolerant protein A-derived ligand allows the use of
0.1 to 0.5 M sodium hydroxide for cleaning-in-place (CIP).
• Improved product quality and reduction in overall costs.
• Eliminates the need for expensive and corrosive CIP reagents.
• High dynamic binding capacity (DBC) reduces process time and
amount of resin used.
• High-flow agarose matrix allows processing of large volumes of
feed.
• Simple scale-up to production-sized BPG, AxiChrom™ and,
Chromaflow™ columns.

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Table of contents
1 Description............................................................................... 3
2 Process development .......................................................... 6
3 Recommended screening conditions........................... 8
4 Removal of leached ligand from final product........10
5 Packing columns..................................................................11
6 Evaluation of column packing........................................22
7 Cleaning-In-Place (CIP) .....................................................25
8 Sanitization ............................................................................26
9 Storage.....................................................................................27
10 Scaling up................................................................................27
11 Troubleshooting ...................................................................28
12 Ordering information.........................................................29

Read these instructions carefully before using the product.

Safety
For use and handling of the product in a safe way, refer to the Safety
Data Sheet.

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1 Description
The protein A-derived MabSelect SuRe ligand is produced in
Escherichia coli. Fermentation and subsequent purification are
performed in the absence of animal products. The ligand has been
specially engineered to create an affinity resin with enhanced alkali
stability and high binding capacity for IgG. The specificity of binding
to the Fc region of IgG is similar to that of conventional Protein A and
provides excellent purification in one step. Alkali tolerance, high
capacity, and low ligand leakage plus the rigid base matrix, make
MabSelect SuRe ideal for the purification of monoclonal antibodies
for clinical applications.
Figure 1 shows stability in alkaline conditions of MabSelect SuRe in
terms of dynamic binding capacity. A pressure/flow curve is shown in
Figure 2.
The characteristics of the resin are summarized in Table 1.

100.0

80.0
Capacity (%)

60.0

40.0
MabSelect SuRe 0.1 M NaOH
MabSelect SuRe 0.5 M NaOH
20.0 MabSelect 0.1 M NaOH

0.0
0 20 40 60 80 100 120

CIP cycles
Fig 1. Dynamic binding capacity for MabSelect™ and MabSelect SuRe after CIP
with 0.1 or 0.5 M NaOH, 15 min contact time, for 0 to 120 cycles

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 Each cycle in Figure 1 consists of:
• 10 column volumes binding buffer, pH 7.4
• 6 column volumes elution buffer, pH 3.0
• 3 column volumes of binding buffer, pH 7.4
• 0.1 or 0.5 M NaOH, 15 min contact time
• 6 column volumes binding buffer, pH 7.4
The dynamic binding capacity (DBC, 10% breakthrough) for
MabSelect SuRe was measured every 20 cycles. The capacity of
conventional Protein A resin (MabSelect) is also included in the
figure for comparison.

800

600
Velocity (cm/h)

400

200

0
0.00 0.10 0.20 0.30

Pressure (MPa)
Fig 2. Typical pressure/flow profile for MabSelect SuRe in a packed bed
(bed height 20 cm) in a BPG 300 column (i.d. 300 mm) in water

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 Table 1. Characteristics of MabSelect SuRe
Matrix Highly cross-linked agarose, spherical
Particle size, d50v1 ~ 85 μm
Ligand Allkali-tolerant, protein A-derived (E.
coli)
Coupling chemistry Epoxy
Dynamic binding capacity, QB10 2 ~ 35 mg hIG/mL resin
Chemical stability Stable to commonly used aqueous
buffers
pH stability, operational3 3 to 12
pH stability, CIP4 3 to 13.7
Cleaning-in-place stability5 0.1 to 0.5 M NaOH
Recommended maximum operating 500 cm/h6
flow velocity
Temperature stability 2°C to 40°C
Delivery conditions 20% ethanol
Storage 2°C to 8°C, 20% ethanol
Regulatory support Regulatory support file is available. No
material of animal origin is used in the
manufacturing process.
1
Median particle size of the cumulative volume distribution
2 Dynamic binding capacity at 10% breakthrough by frontal analysis at a mobile phase
velocity of 500 cm/h in an XK 16/20 column at 20 cm bed height (2.4 min residence time)
for human IgG in 0.020 M NaH2PO4, pH 7.4
3 pH range where resin can be operated without significant change in function
4 pH range where resin can be subjected to cleaning- or sanitization-in-place without
significant change in function
5 See Figure 1 and Section 7 Cleaning-In-Place (CIP).
6 In a BPG 300 column with 30 cm diameter and 20 cm bed height using buffers with the
same viscosity as water at 25°C.

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2 Process development
For initial studies of MabSelect SuRe in small-scale columns, we
recommend prepacked HiScreen™ columns or HiScale™ 10/40
columns with 10 cm bed height. Choose a residence time (see
Table 1, footnote 2) that fulfills your demand on dynamic binding
capacity and nominal flow velocity according to Figure 3. Make sure
the chosen bed height and velocity do not conflict with the large-
scale pressure/flow limitations.
1000

900
2 3
1

800

700
Velocity (cm/h)

4
600
5
500 3
2
400 5
7.5
1

4
300
3 7.5
2 5 10
200
15
7.5 10
100 15

0
0 5 10 15 20 25 30 35 40 45 50
Bed height (cm)
Fig 3. Recommended operating window for MabSelect SuRe (white area).
Choosing bed height and operating velocity in terms of residence time,
pressure restrictions, and large-scale column packing challenges

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 Figure 3 shows the possible combinations of bed height and
operational nominal flow velocity for MabSelect SuRe. The figure
also displays the residence time in the interval 1 to 15 minutes for
any bed height and velocity. Included are also pressure drop
limitations and packing limitations at large-scale. The solid curved
line shows the calculated large-scale column pressure restriction
which is 2 bar according to specification (500 cm/h at 2 bar and
20 cm bed height). The dashed vertical line indicates that operating
at below 10 cm bed height is not favorable. The reason for this is that
large diameter columns have a very different aspect ratio, and that
packing short wide beds is a greater challenge.
Figure 3 can be used as a guide when determining suitable bed
height and operating velocity in terms of residence time and thus
capacity and pressure drop.
Figure 4 shows the relation between dynamic binding capacity and
residence time for MabSelect SuRe.
50.0
DBC at 10% breakrough (mg/mL)

45.0
40.0
35.0
30.0
25.0
20.0
15.0
10.0
5.0
0
0 1 2 3 4 5 6 7

Residence time (min)

Fig 4. Relation between dynamic binding capacity and residence time for
MabSelect SuRe for a purified monoclonal antibody

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3 Recommended screening
conditions
Note: To save material, screening can also be performed using
PreDictor™ plates.
Examples of suitable buffers:
• Buffer A: 20 mM sodium phosphate, 150 mM NaCl, pH 7.2
• Buffer B: 0.1 M sodium citrate, pH 3.0 to 3.6.
Experimental conditions:
• Equilibrate the column with 5 column volumes of buffer A.
• Apply a small sample of antibody.
• Wash the column with 5 column volumes of buffer A.
• Elute the column with a linear gradient of 10 column volumes to
100% buffer B.
• Collect fractions into titrating diluent (e.g., 1.0 M Tris-HCl, pH 8.0
so that the diluent volume equals 5% of the programmed
fraction volume).
• Regenerate the column with 5 to 10 column volumes of 100%
buffer B.
• Wash the column with 3 column volumes of buffer A.
• Perform CIP with 5 column volumes of 0.1 to 0.5 M NaOH.
• Re-equilibrate the column with buffer A.
Washing
To minimize the use of buffer, however, we recommend optimizing
the washing procedure with respect to residence time, volumes, pH,
and conductivity.

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 Elution
When optimizing elution conditions, determine the highest pH that
allows efficient desorption of antibody from the column. This will
prevent denaturing sensitive antibodies due to exposure to low pH.
Stepwise elution (Figure 5) is often preferred in large-scale
applications since it allows the target monoclonal antibody to be
eluted in a more concentrated form, thus decreasing buffer
consumption and shortening cycle times. It might be necessary to
decrease the flow rate due to the high concentrations of protein in
the eluate.
Example
Figure 5 shows an example of purification of a monoclonal antibody
from a clarified mammalian cell culture on MabSelect SuRe. The load
was 21 mg antibody/mL column volume (CV), and the yield was 94%
of highly purified antibody. An XK 16/20 column with a CV of 20 mL
and a bed height of 10 cm was used.
Sample application Elution CIP
3500

3000
Absorbance (mAU)

2500

2000

1500

1000
UV 280 nm
500 pH

0
0 200 400 600 800
Volume (mL)
Fig 5. Purification of a monoclonal antibody from a mammalian cell culture on
MabSelect SuRe

Dynamic binding capacity

The dynamic binding capacity for the target antibody must be
determined by frontal analysis using real process feedstock. The
dynamic binding capacity is a function of the sample residence time
and must therefore be defined over a range of different sample
residence times.

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4 Removal of leached ligand from
final product
The ligand leakage from MabSelect SuRe is generally low. For
example, the eluate from the purification run shown in Figure 5
contained 3 ppm (ng ligand/mg antibody) of leached ligand.
However, in many monoclonal antibody applications it is a
requirement to eliminate leached ligand from the final product.
There are a number of chromatographic solutions, such as cation
and anion exchange chromatography, or multimodal anion
exchange chromatography, which can be used to remove leached
ligand. For more details about removal of leached ligand and
antibody aggregates, see the application note Two step purification
of monoclonal IgG1 from CHO cell culture supernatant (28907892).

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5 Packing columns
Recommended columns
Table 2. Recommended columns for MabSelect SuRe
Column Inner diameter Bed volume1 Bed height (cm)
(mm)
Lab-scale
HiScale 10/40 10 8 to 20 mL6 max 256
HiScale 16/20 16 20 to 40 mL max 20
HiScale 16/40 16 20 to 70 mL max 35
HiScale 26/20 26 53 to 106 mL max 20
HiScale 26/40 26 53 to 186 mL max 35
HiScale 50/20 50 196 to 393 mL max 20
HiScale 50/40 50 196 to 687 mL max 35
Tricorn 5/100 5 2 mL 10
Tricorn 5/150 5 2 to 3 mL max 15
Tricorn 5/200 5 2 to 4 mL max 20
Tricorn 10/100 10 8 mL 10
Tricorn 10/150 10 8 to 12 mL max 15
Tricorn 10/200 10 8 to 16 mL max 20
Production-scale
AxiChrom2 50 to 200 0.2 to 12.5 L max 30
AxiChrom2 300 to 1000 7 to 314 L max 30
BPG3 100 to 300 1 to 28 L max 40
Chromaflow standard4,5 400 to 800 12 to 151 L max 30

1
Bed volume range calculated from 10 cm bed height to maximum bed height.
2
Intelligent Packing method according to MabSelect SuRe can be used.
3 The pressure rating of BPG 450 is too low to use with MabSelect resin.
4
See Application note: Packing MabSelect and MabSelect SuRe resins using verified
methods (11000752).
5 Larger pack stations might be required at larger diameters.
6
Packing methods for bed heights up to 25 cm are provided

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 All large-scale columns can be supplied as variable bed height
columns. Do not choose large diameter columns if the bed height is
low.
For more details about packing HiScale columns, see instructions
HiScale columns (10, 16, 26, 50) and accessories (28967470). For
information on packing of process-scale columns, contact your local
Cytiva representative.

Packing Tricorn columns

Packing preparations
Materials needed
MabSelect SuRe
Plastic spoon or spatula
Glass filter G3
Vacuum suction equipment
Filter flask
Measuring cylinder
Tricorn column
Tricorn packing tube
20% ethanol with 0.2 M NaCl
Equipment
ÄKTA™ system, or a stand-alone pump, depending on the flow rate
required, can be used for packing.
Equilibrate all materials to room temperature.
A pressure relief valve can be attached onto the outlet valve of the
system to avoid drainage of the column at packing. A small back
pressure of 0.02 MPa (0.2 bar, 2.9 psi) is sufficient.
Definitions
The bed height of a gravity settled bed differs from the bed height of
a bed settled at a given flow (consolidated). Therefore, the
compression factor (CF) must be separated from the packing factor
(PF).

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 Lsettled Settled bed height/Gravity settled bed height
Bed height measured after settling by gravity
Lcons Consolidated bed height
Bed height measured after settling the resin at a given
flow velocity
Lpacked Packed bed height
CF Compression factor CF = Lsettled/Lpacked
PF Packing factor PF = Lcons/Lpacked
AC Cross sectional area of the column
VC Column volume VC = Lpacked × AC
Cslurry Concentration of the slurry

Preparing the slurry

To measure the slurry concentration, let the resin settle in
20% ethanol at least overnight in a measuring cylinder. To calculate
the amount of resin to fill into the column, use the following
equation:
V = (AC x Lpacked x (PF + 0.09)) / Cslurry

Equilibrating to packing solution

1 Put a glass filter funnel onto a filtering flask.
2 Suspend the resin by shaking the measuring cylinder and pour
into the funnel.
3 Wash 5 times with 2 column volumes (CV) of packing solution.
Gently stir with a spatula between additions.
4 Pour the washed resin from the funnel into a beaker.
5 Add packing solution to obtain a 50% slurry concentration.

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 Packing the column
Table 3. Main features of the packing method for Tricorn columns

Column Tricorn 5 Tricorn 10

Bed height (cm) 10 10
Slurry/ packing solution 20% ethanol with 0.2 M NaCl
Slurry concentration (%) 50 50
Packing velocity (cm/h) 700 700
Packing flow rate (mL/min) 2.29 9.16
Flow condition (cm/h) 700 700
Flow condition (mL/min) 2.29 9.16

Packing procedure
1 Wet the filters with ethanol and assemble the column according
to Tricorn Empty High Performance Columns (28409488).
2 Attach a packing tube on top of the column tube.
3 Fill the column with slurry suspended in packing solution and top
up with packing solution.
4 Attach a bottom piece, making sure no air is trapped under the
filter.
5 Connect the column top to the pump and start a packing flow
running downwards the column with packing solution. The flow
velocity is shown in Table 3.
6 Pack the column for 10 column volumes.
7 Turn the flow off and attach a stop plug to the column bottom.
8 Dismount the packing tube and remove access resin using a
pipette.
9 Top up the column with packing solution.
10 Attach the adapter. Air in the adapter tubing is displaced by
turning the adapter down until it is 1 to 2 mm above the resin
bed.
11 Start a packing flow running downwards the column with packing
solution. The flow velocity is shown in Table 3. Let the flow run for
5 minutes.

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 12 Mark the bed height and pause the pump.
13 Turn the adapter down to the mark, and then give the adapter an
extra 1/3 turn.
14 Start a conditioning flow running downwards the column with
the packing solution. The flow velocity is shown in Table 3. Let the
flow run for 10 column volumes.
Note: If a gap is formed between the bed and the adapter
during flow conditioning, turn the adapter down to the
bed without stopping the flow.
The column is ready to be tested.

Packing HiScale columns

Packing preparations
Materials needed
MabSelect SuRe
HiScale column
HiScale packing tube (depending on bed height)
Plastic spoon or spatula
Glass filter G3
Vacuum suction equipment
Filter flask
Measuring cylinder
20% ethanol with 0.4 M NaCl
Equipment
ÄKTA system, or a stand-alone pump, depending on the flow rate
required, can be used for packing.
Equilibrate all materials to room temperature.
Definitions
The bed height of a gravity settled bed differs from the bed height of
a bed settled at a given flow (consolidated). Therefore, the
compression factor (CF) must be separated from the packing factor
(PF).

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 Lsettled Settled bed height/Gravity settled bed height
Bed height measured after settling by gravity
Lcons Consolidated bed height
Bed height measured after settling the resin at a given
flow velocity
Lpacked Packed bed height
CF Compression factor CF = Lsettled/Lpacked
PF Packing factor PF = Lcons/Lpacked
AC Cross sectional area of the column
VC Column volume VC = Lpacked × AC
Cslurry Concentration of the slurry

Preparing the slurry

To measure the slurry concentration, let the resin settle in
20% ethanol at least overnight in a measuring cylinder or use the
method for slurry concentration measurement described in
application note 28925932. This method can also be used with
HiScale columns.
Washing the resin
Put a glass filter funnel onto a filtering flask. Suspend the resin by
shaking and pour into the funnel and wash according to the following
instructions:
• 5 times with 5 mL 20% ethanol with 0.4 M NaCl/mL resin
• Gently stir with a spatula between additions.
• Move the washed resin from the funnel into a beaker and add
20% ethanol with 0.4 M NaCl to obtain a 50% slurry
concentration. For HiScale 10/40, a slurry concentration of 58%
is recommended.

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 Packing the column
Table 4. Main features of the packing method for HiScale 10/40

Column HiScale 10/40

Bed height (cm) 10 20 25
Slurry/ packing solution 20% ethanol with 0.4 M NaCl
Slurry concentration (%) 58 58 58
Packing factor (PF) 1.001 1.001 1.001
Packing velocity (cm/h) 1222 1222 1070
Packing flow rate (mL/min) 16 16 14
Flow condition (cm/h) 1222 1222 1070
Flow condition (mL/min) 16 16 14
1) When packing HiScale10/40 columns, the compression of the resin to desired bed
height is done either with a combination of flow and mechanical compression or by
using flow compression only. The latter is applicable for MabSelect Sure. Since the
packing factor in Table 4 refers only to mechanical compression
it is consequently 1.00.

Table 5. Main features of the packing method for HiScale 16/20 and
HiScale 16/40

Column HiScale 16/ HiScale 16/40

20
Bed height (cm) 10 20 35
Slurry/ packing solution 20% ethanol with 0.4 M NaCl
Slurry concentration (%) 50 50 50
Packing factor (PF) 1.10 1.10 1.06
Packing velocity (cm/h) 300 300 300
Packing flow rate (mL/min) 10 10 10
Flow condition (cm/h) 750 450 260
Flow condition (mL/min) 25 15 8.6

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 Table 6. Main features of the packing method for HiScale 26/20 and
HiScale 26/40

Column HiScale 26/ HiScale 26/40

20
Bed height (cm) 10 20 35
Slurry/ packing solution 20% ethanol with 0.4 M NaCl
Slurry concentration (%) 50 50 50
Packing factor (PF) 1.15 1.13 1.10
Packing velocity (cm/h) 300 300 300
Packing flow rate (mL/min) 27 27 27
Flow condition (cm/h) 750 450 260
Flow condition (mL/min) 66 40 23

Table 7. Main features of the packing method for HiScale 50/20 and
HiScale 50/40

Column HiScale 50/ HiScale 50/40

20
Bed height (cm) 10 20 35
Slurry/ packing solution 20% ethanol with 0.4 M NaCl
Slurry concentration (%) 50 50 50
Packing factor (PF) 1.15 1.10 1.06
Packing velocity (cm/h) 300 300 300
Packing flow rate (mL/min) 100 100 100
Flow condition (cm/h) 750 450 260
Flow condition (mL/min) 250 150 86

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 Packing procedure
1 Assemble the column according to the column instructions
(HiScale columns (10. 16, 26, 50) and accessories, 28967470).
2 Put the column tube in a stand.
3 Connect the bottom adapter unit to the pump or a syringe and
prime the bottom net with a slow flow of packing solution. This is
easiest done if the nets are dry but if air is trapped under the net
it can be removed by a light suction with a syringe.
4 Attach the bottom adapter unit in the bottom of the column tube
and tighten the O-ring firmly.
5 Fill the column with approximately 1 cm packing liquid using the
pump/syringe. Disconnect the pump/syringe and put a stop plug
on the outlet.
6 Attach the packing tube on top of the column tube if needed to
achieve the requested bed height.
7 Connect the top adapter to the pump and prime it with a slow
downward flow. The net needs to be facing the roof as this is
done. If air is trapped under the net it can be removed by a light
suction with a syringe.
8 Fill the column with slurry suspended in packing solution. If
needed, top up the slurry with extra packing solution so the top
adapter dips into the slurry to avoid air under the net.
9 Put the top adapter unit on top of the packing tube. Tighten the
O-ring firmly and remove the bottom stop plug.
10 Start a downward flow with .packing velocity according to
Table 4, 5, 6, or 7.
11 Let the flow run until the bed has consolidated.
12 Use the scale on the column to measure the bed height. There
might be a buildup of resin at the column wall after the bed is
consolidated and to easier see where the top of the bed is, a light
source can be used.

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 13 Calculate the final bed height by dividing the consolidated bed
height with the desired packing factor.
Lpacked = Lcons/PF
Note: For HiScale 10/40 columns, the compression of the
packed bed is done either with a combination of flow and
mechanical compression or by using flow compression
only. The latter is applicable for MabSelect Sure. Since the
packing factor in Table 4 refers only to mechanical
compression it is consequently 1.00.
For HiScale 10/40, proceed to Step 22
14 Turn off the flow and put a stop plug in the bottom.
15 Dismount the top adapter from the packing tube.
16 Over a beaker or a sink, detach the packing tube from the
column.
17 Remount the top adapter in the column tube. Make sure no air is
trapped under the net and lower the adapter down to 1 to 2 cm
above the bed, making sure the surface is not disturbed.
18 Tighten the O-ring on the adapter. Remove the bottom stop plug
and carefully start turning the end cap down. While spilling out
liquid through the bottom, proceed turning until the calculated
final bed height is reached.
19 Make sure that the pressure peaks that occur during turning the
end knob down do not exceed the pressure specifications of the
resin.
20 Start a downward flow to flow condition the bed. The flow rate is
shown in Table 4, 5, 6, or 7.
21 Let the flow run for about 10 column volumes.
The column is ready to be tested.

HiScale 10/40
22 For HiScale 10/40, increase the flow to compress and condition
the bed. The flow is shown in Table 4. Continue this phase for 5
column volumes (CV). At the end of this phase, mark the bed
height while keeping up the flow.

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 23 Turn off the flow and untighten the top adapter slightly (release
the pressure on the O-ring) which facilitates adjustment of the
adapter without "twisting" the O-ring. This procedure must be
performed with the bottom tubing open which makes it possible
to monitor the pressure. The pressure that is generated from
turning the adapter down must not exceed the pressure during
the conditioning phase. Continue to turn the adapter down until
reaching the bed height mark.
24 Before starting the conditioning flow, the O-ring must be firmly
tightened.
25 Continue the conditioning phase with 10 CV with the
conditioning flow according to Table 4.
26 The HiScale 10/40 column is now ready to be tested.

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6 Evaluation of column packing
Intervals
Test the column efficiency to check the quality of packing. Testing
must be done after packing, at regular intervals during the working
life of the column, and when separation performance is seen to
deteriorate.

Column efficiency testing

The best method of expressing the efficiency of a packed column is
in terms of the height equivalent to a theoretical plate (HETP) and
the asymmetry factor (As). These values are easily determined by
applying a sample such as 1% acetone solution to the column.
Sodium chloride can also be used as a test substance. Use a
concentration of 0.8 M NaCl in water with 0.4 M NaCl in water as
eluent.
For more information about column efficiency testing, consult the
application note Column efficiency testing (28937207).
Note: The calculated plate number will vary according to the test
conditions and it must only be used as a reference value. It
is important that test conditions and equipment are kept
constant so that results are comparable. Changes of solute,
solvent, eluent, sample volume, flow velocity, liquid pathway,
temperature, etc., will influence the results.

Sample volume and flow velocity

For optimal results, the sample volume must be at maximum 2.5% of
the column volume and the liquid velocity 30 cm/h. If an acceptance
limit is defined in relation to column performance, the column plate
number can be used as one of the acceptance criteria for column
use.

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Method for measuring HETP and As
Calculate HETP and AS from the UV curve (or conductivity curve) as
follows:
L = bed height (cm)
N = number of theoretical plates
HETP = L
N VR = volume eluted from the start of sample
application to the peak maximum
2 Wh = peak width measured as the width of the
N = 5.54 × VR recorded peak at half of the peak height
Wh
VR and Wh are in the same units

The concept of reduced plate height is often used for comparing

column performance.
The reduced plate height, h, is calculated as follows:
HETP
h= d50v = Median particle size of the cumulative
d50v
volume distribution (cm)

As a guideline, a value of < 3 is very good.

The peak must be symmetrical, and the asymmetry factor as close to
1 as possible (a typical acceptable range could be 0.8 < AS < 1.8).
A change in the shape of the peak is usually the first indication of bed
deterioration due to excessive use.
Peak asymmetry factor calculation:

a = ascending part of the peak width at 10% of

As = b peak height
a
b = descending part of the peak width at 10% of
peak height

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 Figure 6 shows a UV trace for acetone in a typical test
chromatogram from which the HETP and As values are calculated.
Absorbance
VR

Wh

50%

a b
10%

Volume

Fig 6. A typical test chromatogram showing the parameters used for HETP
and As calculations

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7 Cleaning-In-Place (CIP)
Cleaning-in-place (CIP) is the removal of very tightly bound,
precipitated or denatured substances from the purification system.
If such contaminants are allowed to accumulate, they can affect the
chromatographic properties of the column, reduce the capacity of
the column and, potentially, come off in subsequent runs. If the
fouling is severe, it can block the column, increase back pressure,
and reduce flow rate.
Regular CIP prevents the buildup of contaminants in the packed bed,
and helps to maintain the capacity, flow properties, and general
performance of MabSelect SuRe. We recommend performing a blank
run, including CIP, before the first run with antibody feed.
MabSelect SuRe is an alkali-tolerant resin allowing the use of NaOH
as CIP agent. NaOH is widely accepted for cleaning due to the low
cost and the ability to dissolve proteins and saponify fats.
For difficult cases were CIP with NaOH is not sufficient to restore the
column performance an extended protocol including wash with 100
mM thioglycerol pH 8.5 followed by CIP with 0.1 to 0.5 M NaOH is
recommended. For more details, see the application note High-
throughput process development for design of cleaning-in-place
protocols (28984564).

CIP protocol
1 Wash the column with 3 column volumes of binding buffer.
2 Wash with at least 2 column volumes of 0.1 to 0.5 M NaOH.
Contact time 10 to 15 minutes.
3 Wash immediately with at least 5 column volumes of sterile and
filtered binding buffer at pH 7 to 8.
CIP is usually performed immediately after the elution. Before
applying the alkaline NaOH CIP solution, we recommend
equilibrating the column with a solution of neutral pH in order to
avoid the direct contact between low-pH elution buffer and high-pH
NaOH solution on the column. Mixing acid and alkaline solutions
might cause a rise in temperature in the column.
NaOH concentration, contact time, and frequency are typically the
main parameters to vary during the optimization of the CIP. The

11002601 AG 25
 nature of the feed material will ultimately determine the final CIP.
However, the general recommendation is to clean the column at
least every 5 cycles during normal use. Depending on the nature of
the contaminants, different protocols maybe have to be combined,
for example 0.1 M NaOH every cycle and 0.5 M NaOH every 10 cycles.

8 Sanitization
Sanitization reduces microbial contamination of the
chromatographic bed to a minimum. MabSelect SuRe is
alkali-tolerant allowing the use of NaOH as sanitizing agent.
NaOH is very effective for inactivating viruses, bacteria, yeasts, and
endotoxins. In addition, NaOH is inexpensive compared with other
sanitizing agents.

Sanitization protocol
1 Wash the column with 3 column volumes of binding buffer.
2 Equilibrate the column with 0.1 to 0.5 M NaOH.
3 Use a contact time of at least 15 minutes for 0.5 M NaOH or
30 minutes for 0.1 M NaOH (see also the note below).
4 Wash immediately with at least 5 column volumes of sterile and
filtered binding buffer at pH 7 to 8.
For more challenging microbial contamination, a mixture of 30% to
40% 1- or 2-propanol in 0.5 M NaOH could be used for sanitization.
Note: Higher concentrations of NaOH and/or longer contact time
inactivates microorganisms more effectively. However,
these conditions might also lead to a decrease in the
dynamic binding capacity. The conditions for sanitization
must therefore be evaluated to maximize microbial killing
and to minimize loss of capacity.

26 11002601 AG
9 Storage
Store unused resin in its container at a temperature of 2°C to
8°C. Make sure that the screw top is fully tightened.
Equilibrate packed columns in buffer containing 20% ethanol or 2%
benzyl alcohol to prevent microbial growth.
After storage, equilibrate with starting buffer and perform a blank
run, including CIP, before use.

10 Scaling up
After optimizing the antibody fractionation at laboratory-scale, the
process can be scaled up to pilot- and process-scales.
• Keep the residence time constant in order to maintain the
dynamic binding capacity.
• Select bed volume according to required binding capacity. Verify
the purification step with the new bed height, if it is changed.
• Select column diameter according to your volume throughput
requirements. Then determine the bed height to give the
desired residence time. Bed heights of 10 to 25 cm are generally
considered appropriate. Note that the backpressure increases
proportionally with increasing bed height at constant nominal
velocity.
• Keep sample concentration and elution conditions constant.
See also Figure 3 for appropriate windows of operation for
MabSelect SuRe.

11002601 AG 27
11 Troubleshooting
The list describes faults observed from the monitor curves.
Fault Possible cause/corrective action
High backpressure during • Change the in-line filter.
the run • The column is clogged. Perform CIP.
• The adapter net/filter is clogged. Clean or
replace the net/filter.
Unstable pressure curve • Remove air bubbles that might have
during been trapped in the sample pump.
sample application • Degas the sample using a vacuum
degasser or an air trap.
Gradual broadening of the • Might be due to insufficient elution and
eluate peak CIP caused by contaminants
accumulating in the column. Optimize
the elution conditions, the CIP protocol
and/or perform CIP more frequently.
• Perform wash with thioglycerol followed
by CIP with NaOH, according to
Section 7.
Gradual decrease in yield • Too high sample load. Decrease the
sample load.
• Precipitation during elution. Optimize the
elution conditions.
• Might be due to insufficient elution and
CIP. Optimize the elution conditions, the
CIP protocol and/or perform CIP more
frequently.
Gradual increase in CIP • Might be due to insufficient elution or
peaks CIP. Optimize the elution conditions, the
CIP protocol and/or perform CIP more
frequently.
• Perform wash with thioglycerol followed
by CIP with NaOH, according to
Section 7.
High ligand leakage during Perform a blank run, including CIP, before
the first purification cycle the first purification cycle on a new column.

28 11002601 AG
12 Ordering information
Product Quantity Product code
MabSelect SuRe 25 mL 17543801
200 mL 17543802
1L 17543803
5L 17543804
10 L 17543805

Related product Quantity Product code

HiTrap™ MabSelect SuRe 1 × 1 mL 29049104
5 × 1 mL 11003493
1 × 5 mL 11003494
5 × 5 mL 11003495
HiScreen MabSelect SuRe 1 × 4.7 mL 28926977
PreDictor MabSelect Sure, 6 μL 4 × 96-well filter plates 28925823
PreDictor MabSelect Sure, 20 μL 4 × 96-well filter plates 28925824
PreDictor MabSelect Sure, 50 μL 4 × 96-well filter plates 28925825
HiScale 10/40 1 29360550
HiScale 16/20 1 28964441
HiScale 16/40 1 28964424
HiScale 26/20 1 28964514
HiScale 26/40 1 28964513
HiScale 50/20 1 28964445
HiScale 50/40 1 28964444
Tricorn 5/100 1 28406410
Tricorn 5/150 1 28406411
Tricorn 5/200 1 28406412
Tricorn 10/100 1 28406415
Tricorn 10/150 1 28406416
Tricorn 10/200 1 28406417

11002601 AG 29
Accessories Quantity Product code
Tricorn Glass Tube 5/100 1 18115306
Tricorn Packing Connector 5-5 1 18115321
Tricorn Packing Equipment 10/100 1 18115325
Packing tube 20 (HiScale 10) 1 29360551
Packing tube 20 (HiScale 16) 1 28986816
Packing tube 40 (HiScale 16) 1 28986815
Packing tube 20 (HiScale 26) 1 28980383
Packing tube 40 (HiScale 26) 1 28964505
Packing tube 20 (HiScale 50) 1 28980251
Packing tube 40 (HiScale 50) 1 28964506

Related literature Product code

Data Files MabSelect SuRe 11001165
AxiChrom Columns 28929041
BPG columns 18111523
Chromaflow columns 18113892
HiScale columns 28975523
Tricorn empty High Performance columns 18114736
Application notes MabSelect SuRe – Leakage and Toxicity 11001164
MabSelect – Column packing 11000752
Two step purification of monoclonal IgG1 from 28907892
CHO cell culture supernatant
High-throughput process development for 28984564
design of cleaning-in-place protocols

30 11002601 AG
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11002601 AG 31
cytiva.com
Cytiva and the Drop logo are trademarks of Global Life Sciences IP Holdco LLC or an affiliate.
ÄKTA, AxiChrom, BioProcess, Chromaflow, HiScale, HiScreen, HiTrap, MabSelect, MabSelect SuRe,
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All other third party trademarks are the property of their respective owners.
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11002601 AG 06/2020