Dr. Ahmed Akrem: Qualifications
Dr. Ahmed Akrem: Qualifications
AHMED AKREM
Qualifications:
Ø HPLC, FPLC, PCR, Gene Cloning and Expression, Transformation (gene gun,
Electroporation) Spectrophotometry, Native, SDS-PAGE, Activity staining &
Fermentation biotechnology, X-ray Crystallography (Imosflm; Scala, Denzo;
Scalepack, CCP4i Suite, Molecular Replacement; Molrep, Phaser, Mrbump, Homer,
COOT, Refmac5, Protein Data Bank, Pdbsum, Pdb goodies, Chimera, Pymol, Auto-
Rickshaw).
Publications:
Ø Purification and Characterization of a Xylanase Produced by Chaetomium
thermophile NIBGE. Farooq Latif, Muhammad Asgher, Rabia Saleem, Ahmed
Akrem and R.L. Legge. World Journal of Microbiology & Biotechnology (2006)
22:45–50 IF: 1.2
Ø Isolation, purification, crystallization and preliminary crystallographic studies of a
chitinase from Crocus vernus. A. Akrem, S. Iqbal, F. Buck, A. Meyer, M. Perbandt,
W. Voelter and C. Betzel. Acta Cryst. (2011). F67, 340-343 IF: 0.56
Protein Data Bank ID
Ø Crystallographic structure analysis of family 18 Chitinase from Crocus vernus.
Akrem, A., Iqbal, S., Buck, F., Negm, A., Perbandt, M., Betzel, C. PDB ID: 3SIM
Ø X-ray structural analysis of a mannose specific lectin from dutch Crocus (Crocus
vernus). Akrem, A., Meyer, A., Perbandt, M., Voelter, W., Buck, F., Betzel, C.
PDB ID: 3MEZ
Ø Crystal structure of Cysteine protease from bulbs of Crocus sativus at 1.3 Å
resolution. Iqbal, S., Akrem, A., Buck, F., Perbandt, M., Banumathi, S., Betzel, C.
PDB ID: 3U8E
Ø One Lectin-Mannose complex is still to deposit.
Abstract:
Ø Crystallization and structure determination of a C. sativus Cysteine protease. Sadaf
Iqbal, Ahmed Akrem, Friedrich Buck, Markus Perbandt, Sankaran Banumathi, M.
Iqbal Choudhary, and Christian Betzel. 27th European Crystallographic Meeting.
August 6-11, 2012. Bergen, Norway.
References:
1. Professor Dr. Dr. Christian Betzel
Deutsches Elektronen Synchrotron (DESY)
University of Hamburg, Germany
Ph: No: 040 / 42838 - 2840/6069, Fax: - 7818
E-Mail: [email protected]