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1) Enzymes are proteins that act as catalysts to lower the activation energy needed for chemical reactions. They bind to substrates at an active site, positioning them for efficient reactions. 2) Amylase is a digestive enzyme produced by the pancreas and salivary glands that breaks down starches and is used to diagnose pancreatic diseases. Elevated amylase levels along with abdominal pain and imaging findings indicate pancreatitis. 3) This lab analyzes the effects of temperature, substrate concentration, enzyme concentration, and inhibitors on the rate of an enzyme reaction between catalase and hydrogen peroxide. Increasing these factors increases the reaction rate, while an inhibitor decreases it.

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71 views

Bio

1) Enzymes are proteins that act as catalysts to lower the activation energy needed for chemical reactions. They bind to substrates at an active site, positioning them for efficient reactions. 2) Amylase is a digestive enzyme produced by the pancreas and salivary glands that breaks down starches and is used to diagnose pancreatic diseases. Elevated amylase levels along with abdominal pain and imaging findings indicate pancreatitis. 3) This lab analyzes the effects of temperature, substrate concentration, enzyme concentration, and inhibitors on the rate of an enzyme reaction between catalase and hydrogen peroxide. Increasing these factors increases the reaction rate, while an inhibitor decreases it.

Uploaded by

Rana bilal
Copyright
© © All Rights Reserved
Available Formats
Download as PDF, TXT or read online on Scribd
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Introduction

Enzyme is catalyst. It is normally a protein. They decrease the Activation energy of a response,
that required to complete a reaction (Boncmpagni, Tatiana. New York Times, 22 Feb. 2012. Web. 22
Oct. 2013). They do that with the aid of using binding to a substrate and preserving it in a manner
that lets in the response to show up greater efficiently. a part of the enzyme wherein the
substrate binds is referred to as Active site Boncmpagni, Tatiana. New York Times, 22 Feb. 2012.
Web. 22 Oct. 2013). The enzymes change there shapes slightly according to the required
condition, binds slightly with substrate and make a complex enzyme Boncmpagni, Tatiana. New
York Times, 22 Feb. 2012. Web. 22 Oct. 2013).

Amylase basically a enzyme. It is found in the human body. Also, it is a digesting enzyme.
enzyme predominantly secreted via way of means of the pancreas and salivary glands and
discovered in different tissues in very small levels. Amylase is normally utilized in diagnosing
pancreatic diseases. Amylase is a usually measured enzyme because of the supply of
inexpensive, without difficulty computerized methods. Although amylase is a touchy indicator
of acute pancreatitis, it isn't precise as it could be expanded in numerous situations unrelated
to the pancreas. Pancreatitis may be described via way of means of out of the 3 following
criteria: stomach pain, serum amylase and/or lipase ranges extra than 3 instances the top
restriction of normal, and stomach imaging assisting feature findings of pancreatitis.

These variable reasons an instantaneous impact at the structured variable. These variable
changes with the dependent variables. The dependent variable is the reaction speed
(Gastroenterol Clin North Am. 1990 Dec). The independent variable is the enzyme
concentration, the substrate concentration, the temperature and the addition of an inhibitor
(Gastroenterol Clin North Am. 1990 Dec).
The motive of this lab is to analyse the effect of temperature, substrate attention, enzyme
attention, and the presence of an inhibitor at the effectiveness and charge of an enzyme. If the
attention of the enzyme or substrate is extended, the charge of the response could be extended
as well. If the temperature of the enzymes surrounding surroundings is extended, then the
charge of the response can also be extended. If the substrate attention is extended then the
charge of the response will increase (Koshland, Daniel E22 Oct. 2013). If an inhibitor is
introduced then the charge of the response could be reduced significantly.
Once all these statistics became calculated and, investigated, and analysed, it became obtrusive
the objectives and functions of the lab had been attained. This goal became to decide the results
of substrate concentration, enzyme concentration, temperature, and an inhibitor at the price of
a response. The final conclusions made had been that as enzyme concentration, substrate
concentration, and temperature had been increased, the price of the response additionally
increased (Koshland, Daniel E., Jr. "Role of Enzymes in Metabolism." Encyclopedia Britanica.
Ecyclopedia Britanica Inc., n.d. Web. 22 Oct. 2013.). When the inhibitor became delivered the
price of the response reduced significantly. All 4 of those components of the test used the
response among catalase and hydrogen peroxide.
References
1) Boncmpagni, Tatiana. "Enzymes Try to Grab the Spotlight." New York Times. New
York Times, 22 Feb. 2012. Web. 22 Oct. 2013.
2) Pieper-Bigelow C, Strocchi A, Levitt MD. Where does serum amylase come from and
where does it go? Gastroenterol Clin North Am. 1990 Dec;19(4):793-810
3) Koshland, Daniel E., Jr. "Role of Enzymes in Metabolism." Encyclopedia Britanica.
Ecyclopedia Britanica Inc., n.d. Web. 22 Oct. 2013.

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