Grade 12 Practical Term - 1

PORTION FOR TERM -1 PRACTICAL Portion for viva
1.VOLUMETRIC ANALYSIS -4 MARKS Learn lesson

2.SALT ANALYSIS- 4 MARKS Biomolecules
3.CONTENT BASED EXPERIMENT-2
4.CLASS RECORD AND VIVA-5
EXP I. Volumetric Analysis

KMnO4 vs Mohr’s salt solution
Aim: Determine the strength of given KMnO4 solution You are provided with
M/20 Mohr’s salt solution
Ionic equations: MnO4 - + 8H+ + 5e - → Mn2+ + 4H2O
Fe2+ → Fe3+ + e-] x 5
MnO4- +8H+ + 5Fe2+ → 5Fe3+ + Mn2+ + 4H2O
Indicator: KMnO4 is a self-indicator

End point: Colourless to permanent pink colour
Procedure:20 mL of Mohr’s salt solution is pipetted out in a conical flask.20 mL of
dilute sulphuric acid solution is added to it.
Rinse and fill the burette with the given KMnO4 solution.
Note the initial reading of the burette.
Add KMnO4 solution from the burette till the end point.
End point of titration: Colourless to permanent light pink colour
Repeat the experiment to get concordant readings.
OBSERVATIONS and CALCULATIONS
Sl. No Vol of Mohr’s IBR FBR Volume of

salt solution KMnO4
1 20 mL 0 21mL 21mL
2 20 mL 0 21mL 21mL
3 20 ml 0 21mL 21mL
[Final burette readings in exam to be written after performing the experiment.]

Volume of KMnO4 = 21 mL
Volume of Mohrs salt solution = 20 mL
Molarity of Mohrs salt solution = 1/20 M
Molarity of KMnO4 = ?
Molarity of KMnO4 is calculated using the formula
MKmnO4 x VKMnO4 = 2
MFeSO4 x VFeSO4 10
MKMnO4 x 21 = 2
1/20 x 20 10
M KMnO4 = 2 = 0.0095 M
21x 10
Strength of given KMnO4 solution = Molarity x Molar mass = 0.0095 x 158
= 1.501 g/L
EXP II : SALT ANALYSIS
SALT ANALYSIS-1 : Ammonium Carbonate

SlNo Experiment Observation Inference
1. Noted the colour of the salt White Cu2+, Fe2+ ,Fe3+ ,Ni2+
Mn2+, Co2+ absent
2. Flame test No characteristic Absence of
Prepared a paste of the salt colour Ca2+,Sr2+,Ba2+
with Con:HCl and performed Cu2+, Mn2+
flame test
Analysis of Acid radicals
Dil.Sulphuric acid test Brisk effervescence presence of carbonate ion.
3. 1.CO3 2-[Carbonate ] : Add with the evolution of
dilute H2SO4 to the salt CO2 that turns lime
solution. water milky
4. Confirmatory test for carbonate
ion
To the salt solution add MgSO4 White precipitate presence of carbonate ion
solution formed confirmed
Analysis of basic radical
5. Salt is heated with NaOH Smell of ammonia gas Presence of NH4+

solution.
Glass rod dipped in White fumes produced Presence of NH4+

Con HCl is shown to the gas
evolved in the above
experiment
Confirmatory test for NH4+
Salt soln +NaOH and Nessler’s Brown precipitate
6. reagent added
Presence of NH4+
confirmed
RESULT : The given salt contains NH4+ basic radical and CO32- acidic radical
SALT ANALYSIS-2 Ammonium Chloride
Mn2+, Co2+ absent
2. Flame test No characteristic colour Absence of
Prepared a paste of the salt Ca2+,Sr2+,Ba2+
with Con:HCl and performed Cu2+, Mn2+
flame test
3. Dil.Sulphuric acid test
1.CO3 2-[Carbonate ] : Add No Brisk effervescence Absence of carbonate ion.
dilute H2SO4 to the salt
solution.
4. To aqueous solution of the salt White precipitate soluble Presence of Chloride ion
DilHNO3+AgNO3 added in NH4OH formed
Confirmatory test for chloride
ion
Heat a pinch of the salt with a Evolution of greenish Presence of Cl- confirmed
5. small quantity of MnO2 and yellow gas having a
Con:H2SO4 pungent irritating smell

6. Salt is heated with NaOH Smell of ammonia gas Presence of NH4+
solution.
Glass rod dipped in White fumes produced
Con HCl is shown to the gas Presence of NH4+
evolved in the above
experiment
Confirmatory test for NH4+
Salt soln +NaOH and Nessler’s
reagent added Presence of NH4+
7. Brown precipitate confirmed
RESULT : The given salt contains NH4+ basic radical and Cl- acidic radical
SALT ANALYSIS- 3 Aluminum Chloride
Mn2+, Co2+ absent
2. Flame test No characteristic colour Absence of
Prepared a paste of the salt with Ca2+,Sr2+,Ba2+
Con:HCl and performed flame test Cu2+, Mn2+
3. Dil.Sulphuric acid test
1.CO3 2-[Carbonate ] : Add dilute No Brisk effervescence Absence of carbonate ion.
H2SO4 to the salt solution.
4. To aqueous solution of the salt White precipitate soluble Presence of Chloride ion
DilHNO3+AgNO3 added in NH4OH formed
Confirmatory test for chloride ion
Heat a pinch of the salt with a small Evolution of greenish Presence of Cl- confirmed
quantity of MnO2 and Con:H2SO4 yellow gas having a
5. pungent irritating smell
6. Salt is heated with NaOH solution. No Smell of ammonia gas Absence of NH4+
Salt solution +DilHCl No White precipitate
7. Absence of group I
Salt solution +DilHCl and pass H2S No precipitate radicals
8. gas Absence of group II
Salt solution +pinch of ammonium White gelatinous radicals
9. chloride + precipitate Presence of Al3+
Excess of NH4OH
Confirmatory test for Al3+ White precipitate formed
Salt solution +NaOH Presence of Al3+confirmed
RESULT: The given salt contains Al3+ basic radical and Cl- acidic radical
EXPERIMENT NO -III:CONTENT BASED EXPERIMENT
Test for carbohydrate ,fat and protein
Experiment Observation Inference
Test for carbohydrate A shining silver mirror Presence of reducing
Take 2-3 mL aqueous carbohydrate
aqueous solution of
carbohydrate. Add 2-3 mL
Tollens reagent. Keep the
test-tube in boiling water for
10 minutes
Test for starch Appearance of blue Presence of starch
To aqueous suspension of colouration
the sample ,add 1-2 drops of
iodine solution
Test for fat/oil A pungent irritating odour of Presence of oil and fat
Heat a little of the sample acrolein
with some crystals KHSO4 in
a test tube
Test for proteins Bluish violet colour Presence of protein
Add 2 ml of NaOH solution
&4-5 drops of 1% CuSO4
solution Warm the mixture
for five minutes

Grade 12 Practical Term - 1

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PORTION FOR TERM -1 PRACTICAL Portion for viva

1.VOLUMETRIC ANALYSIS -4 MARKS Learn lesson

EXP I. Volumetric Analysis

Indicator: KMnO4 is a self-indicator

Sl. No Vol of Mohr’s IBR FBR Volume of

[Final burette readings in exam to be written after performing the experiment.]

SALT ANALYSIS-1 : Ammonium Carbonate

5. Salt is heated with NaOH Smell of ammonia gas Presence of NH4+

Glass rod dipped in White fumes produced Presence of NH4+

Analysis of basic radical

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