R.Espinar, S.L.: Instruction Manual For Use and Maintenance
R.Espinar, S.L.: Instruction Manual For Use and Maintenance
AESi/09
R.ESPINAR, S.L.
INSTRUCTION MANUAL
FOR USE AND MAINTENANCE
"MICROPROCESSOR CONTROLLED”
Models:
AES-12
AES-28
AES-75
AES-110
AES-150
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This manual has been written for safety reasons, read the instructions
carefully before installing and /or using this apparatus.
If this apparatus should be sold or transferred, ensure that this manual
is given to the new owner, for the correct use and installation.
This manual should be kept by the apparatus for in case of doubt in its
use and for maintenance reasons.
Explosive substances
- Nytroglycol, nitroglycerin, nitrocellulose, and other explosive nitric esters.
- Trinitrobenzene, trinitrotoluene, picric acid, and other explosive nitro compounds.
- Peracetic acid, methyl ethyl ketone peroxide, benzoyl peroxide and other organic
peroxides.
Ignitable substances
- Metallic lithium, potassium, sodium, yellow phosphorous, phosphorous sulfide, and
red phosphorous.
- Celluloids, calcium carbide (carbide), lime phosphide, and magnesium powder.
- Aluminum powder, magnesium powder, and metallic powders other than aluminum
powder.
- Sodium dithionite (or sodium hydrosulfite).
Oxidizing agents
- Potassium chlorate, sodium chlorate, ammonium chlorate, and other chlorates.
- Potassium perchlorate, sodium perchlorate, ammonium perchlorate, and other
perchlorates.
- Potassium peroxide, sodium peroxide, barium peroxide, and other inorganic
peroxides.
- Potassium nitrate, sodium nitrate, ammonium nitrate, and other nitrates.
- Sodium chlorite and other chlorites.
- Calcium hypochlorite and other hypoclorites.
Flammable substances
- Ethyl ether, gasoline, acetaldehyde, propylene oxide, carbon disulfide, and other
substances whose flash points range from -30 to 0ºC.
- Methanol, ethanol, xylene, benzyl acetate (or amyl acetate), and other substances
whose flash points range from 0 to 30ºC.
- Kerosene, gas oil, turpenine oil, isopentyl alcohol (or isoamyl alcohol), acetic acid,
and other substances whose flash points range from 30 to 65ºC.
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Flammable gas
- Hydrogen, acetylene, ethylene, methane, ethane, propane, butane, and other
substances that are gases at temperature of 15ºC under 1 atmospheric pressure.
2) When liquid whith salt water and much salinity of salt agar, etc. spills in the chamber,
blowing, discharge water in the chamber and wipe up drop of water around the lid
gasket beautifully. It causes the corrosion of the chamber and the piping when
leaving just as it is.
3) Absolutely do not attempt to remodel or alter this product.
4) Do not forcibly bend, twist, tie or extend the power cord. Do not place heavy objects
on the cord. A damaged cord or exposed wire can cause fire or electric shock.
5) Never connect the power cord to a power supply other than one of the rated voltage.
Connection to such a power supply can cause fire or electric shock.
6) If grounded socket is unavaliable, ground the equipment using a separate ground
wire before connecting the power cord to the power source.
7) Never ground to a gas pipe or vinyl chloride water service pipe.
8) Do not pour anything except for water.
9) Do not use the autoclave for the purpose other than sterilization and agar preparation
(dissolution).
10) Close the lid after confirming that no foreign matter is adhering to the section
contacting the lid gasket. Foreign matter in this section can cause vapor leaks.
11) When using a waste processing bag or other kind of bag and disinfecting, place the
bag in the metal mesh holder and then insert it into the chamber. Using the bag “as
is” can cause excessive temperatures, pressures, lack-of-water, etc.
12) Do not put your face or hands close to the chamber when lifting the lid after
operations are complete; steam will gush out of the chamber.
13) The lid, chamber, gasket, and top casing are extremely hot immediately after the
completion of operation. Do not touch the equipment or you may get burned.
14) Put on heat insulating gloves before removing a substance from the chamber. Do not
put hands into the chamber until the steam has been vented.
15) Some time is required for liquids to cool. Be sure to check that the temperature has
dropped sufficiently before unloading a liquid from the chamber or burns can result.
16) Never drain the chamber when the chamber is under pressure. Boiling water or
steam may gush out causing burns.
17) If any abnormality occurs (e.g. abnormal sounds, smells, smoke), immediately shut
the power off. After checking that the abnormal condition does not continue, call out
authorized distributor in your region.
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INDEX
Description ............................................................................……......…........................ page 5
Rear view ..........................................................................................….......................... page 5
Microprocessor functions and characteristics …........................ .................…..................... page 6
Technical specifications ….................................................................................................. page 7
Characteristics ................................................................................................................. page 7
Accessories .....................................................................…........................................... page 8
Installation .......................................................................…............................................ page 9
Connection to the mains .........................................................…...................................... page 9
Changing voltage ........................................................................................................… page 9
Programming mode ...........................................................................…........................... page 10
Alphanumeric screen functions ...........................................................…........................ page 11
Program introduction ..........................................................................…......................... page 12
Date and hour adjust ....................................................................................................... page 12
Selection of Language and type of degree ......................................................................... page 13
Start up ............................................................................................................................ page 13
Shut down of the autoclave ................................................................................................. page 16
Loading the autoclave ............................. ……………..................................................... page 17
Printer mode .................................................................................................................. page 17
Protected programs (info.) ......................................................................................... page 20
Warning messages .................…….................................................................................. page 20
Failure massages ....…................................................................................................... page 20
Cleaning and maintenance ................................................................................................ page 21
Repairs and spare parts ................................................................…............................... page 22
Guarantee ........................................................................................................................ page 22
Conformity declaration “CE” ............................................................................................ page 23
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DESCRIPTION 2
2
4 5
3
1. Drain valve
5
1 3 4
2. Closing wheel.
3. Microprocessor. 1. Microprocessor
4. Pressure and vacuum gauge. 2. Closing wheel
5. Main switch. 3. Main switch
6. Thermal protection of the lid. 4. Pressure and vacuum gauge
7. Exhaust valve. 5. Three position valve
REAR VIEW
9 6 7 8
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4
5
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TECHNICAL SPECIFICATIONS
Apparatus: Steam sterilization autoclave
Sterilization temperature: From 100ºC to 139ºC.
Max. pressure: 2,5 bar
Voltage: 220 V single-phase (for AES-12 , AES-28 and
AES-75) 220 V or 380 V triple phase + N (for
AES-110 and AES-150)
Frequency: 50/60 Hz
Consume: 1.000 W (Model AES-12)
2.000 W (Model AES-28)
3.000 W (Model AES-75)
4.500 W (Model AES-110)
6.000 W (Model AES-150)
Sterilization time: from 3 to 120 minutes.
Data printing: Manual/Automatic. (Only for autoclaves
with printer IT.)
Printing cadence: Selection able between 10 and 240 seconds.
CHARACTERISTICS
-Tank and lid made of stainless steel AISI 304.
-Exterior metallic made of AISI-304 stainless steel.
-Seal of rubber and silicone.
-Automatic air purge governed by a microprocessor.
-Safety devices for over temperature, overpressure and safety valve.
-Electric heater for heating element shielded in the chamber itself
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ACCESSORIES
ACCESSORIES THAT MUST BE INSTALLED AT THE FACTORY:
Code Ref. Description
90131300 IT Printer of continuous sheet, that prints the number of
the program, number of cycle, temperature, time and date
of every sterilization.
Other accessories:
Code Ref. Description
90130112 CI-12 Perforated stainless steel basket AISI-304 of
Ø225x1150mm for AES-12
90130228 CI-28 Perforated stainless steel basket AISI-304 of
Ø280x200mm for AES-28. (2 per autoclave)
90130375 CI-75 Perforated stainless steel basket AISI-304 of
Ø380x260mm for AES-75 and for AES-110. (2 per
autoclave AES-75 and 3 per AES-110)
90130450 CI-150 Perforated stainless steel basket AISI-304 of
Ø475x360mm for AES-150. (2 per autoclave)
90130512 CCI-12 Non-perforated stainless steel basket AISI-304 of
Ø225x2150mm for AES-12. (2 per autoclave)
90130628 CCI-28 Non-perforated stainless steel basket AISI-304 of
Ø280x200mm for AES-28. (2 per autoclave)
90130775 CCI-75 Non-perforated stainless steel basket AISI-304 of
Ø380x260mm for AES-75 and AES-110. (2 per autoclave
AES-75 and 3 per AES-110)
90130850 CCI-150 Non-perforated stainless steel basket AISI-304 of
Ø475x360mm for AES-150. (2 per autoclave)
90130740 CEP-7040 Pippete sterilize cylinder of Ø70x400mm
90131340 CEP-1340 Pippete sterilize cylinder of Ø130x400mm
90131326 CEP-1326 Petri capsules sterilize cylinder of Ø130x260mm
90131347 CEP-1347 Petri capsules sterilize cylinder of Ø130x400mm
90131428 SRA-1 Height adjustable support with shelves for AE/AES-28
90131475 SRA-2 Height adjustable support with shelves for AE/AES-75
90131510 SRA-3 Height adjustable support with shelves for AE/AES-110
90131550 SRA-4 Height adjustable support with shelves for AE/AES-150
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INSTALLATION
Install the autoclave on a stable and solid surface.
Don’t install the autoclave in areas where there is danger of fire or
explosion.
Don’t block or cover the rear ventilation grid.
Connect a flexible tube to the drainage rear outlet for drainage.
CHANGING VOLTAGE
To change voltage into 220V triple phase and ground follow the next steps:
-Disconnect the autoclave from the mains
-Take off the back cover of the autoclave
-Change the metallic bridges on the binding post from the star connection to a delta
connection, as indicates below.
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-Take out the neutral (blue) from terminal space of main entrance, and connect to
black wire L1.
-To connect to the net-work, without changing, use the 3 pole, neutral and ground
plug. Leave the neutral binding post out of use.
PROGRAMMING MODE
The microprocessor of this autoclave has 10 programs, numbers from P0 to
P9. First 4, from P0 to P3, are protected programs and they come programmed
from the factory with the usual parameters for sterilization.
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P0 115 0,6 60
P1 121 1,1 30
P2 133 2,1 20
P3 121 1,1 20
P7 121ºC 020min.
The alphanumeric screen apart from showing the sterilization parameters, also
shows a warning, failure and other messages. See WARNING MESSAGES and
FAILURE MESSAGES.
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PROGRAMME INTRODUCTION
To edit data of a program is necessary to start from an initial situation of controller
shown and follow the next steps:
- Connect the autoclave to the mains, and press the main switch position I and at
the same time will light.
- The microprocessor screen will show the message: “RAYPA STERICLAV-S”
during 2 seconds.
When the autoclave is running for the first time, the screen will show in the first
line the nº of the program, the temperature of sterilization and the sterilization
time.
- Select with the buttons (6) “INCREASE” and (7) “DECREASE”, the number of
the desired program.
- Press the switch nº (8) “PRO” during 2 seconds to get into the program editor
desired, and it will be shown the letter “P” to indicate the edition situation.
- Press the switch nº (4) “THERMOMETER” to edit the sterilization data.
- Press the switch nº (5) “CLOCK” to edit the sterilization time.
- Once the field to edit is set, use “Increase” and “Decrease” switches to modify the
values of edition.
- To validate the modified data, press “PRO” switch, finishing the program edition.
- If it is needed to go out from the program without validating the modifications
done, press the “START” switch that is used as an exit switch of the program, the
controller goes back to the normal situation without validating any change in
edition.
- Switches “INCREASE” and “DECREASE”, make no effect over the edition data, if
the selected program is protected.
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START UP INSTRUCTIONS
FILLING
Open the autoclave's lid by turning the closure wheel (2) up to its top, and then turn
the arm to your direction.
Fill in the autoclave's water tank with distilled water up to the lower grid level (see
figure 1)
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Make sure that the taps (1) and (7) are correctly closed (turning the handles 1/4
round up to the end). The three position tap in the model AES-12 (5) will be in the
“cerrado” (off) position.
Take care that the water don't overflow the lower grid
Put in the autoclave the materials to be sterilized, directly or with baskets, so that
there are empty places between these material to circulate the steam. (see LOAD
DISTRIBUTION).
Pay attention not to close the hole in the upper part of inside the autoclave.
Close the lid of the autoclave by means of tighten the closure.
Press the main button and it will light, and also the microprocessor screen showing
the last program executed.
Select the appropriate program, press “START/STOP” switch, initializing the process
of sterilization.
The microprocessor screen will keep you informed of every step of the process.
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STERILIZATION
Before initializing a sterilization program, the microprocessor screen will show the
sterilization chamber temperature in the upper line, and in the lower “PURGE HEATING”.
From that point the temperature will be increasing and purging the air automatically.
By the drainage terminal the water drops will flow through the air condensation, that means
is better to put under a small container (tray) or drive it to a drainage not higher than 14 cm.
from the floor.
Do not use a plastic tube to drive the drainage, because the steam could make loose its
shape., otherwise you can use silicone, latex, cupper tubes, etc.
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Once the air of inside the chamber is purged totally, the message "PURGE" from the
screen will disappear, it will only stay the message "HEATING", and the temperature
will raise up to the programmed one., when it will appear in the screen the message:
"STERILIZING", and in the upper line will be shown a countdown of the selected
sterilization time.
Once the air purge period is finished, and until reaching the working temperature, the
manometer will be increasing the lecture pressures, showing the one inside the
chamber.
When the sterilization time is finished, it will appear in the screen the message:
"COOLING", during this phases, the pressure and the temperature will decrease
naturally, up to the temperature of 95ºC when it will appear on the screen a
message: "END OF PROCESS" and it will sound an acoustic signal for some
seconds.
DO NOT OPEN THE AUTOCLAVE UNTIL THE END OF THE PROCESS AND THE
MANOMETRE WILL SHOW "0".
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PRINTER MODE
The microprocessor comes out from the factory with the configuration of manual
printer.
To make possible printing a sterilization inform, if the printer has a switch called
“PRINT”, will only have to press it.
To print an inform manually from the microprocessor:
1.- Keep pressed switch “TEMPERATURE” (7), during some seconds, in the screen
will appear the message :”MANUAL PRINTING”.
2.- Press the switch (11) “PRO”, in the screen will appear: “PRINT NOW? NO”.
3.- Press the switch (9) “INCREASE”, in the screen will appear: “PRINT NOW? YES”
4.- Press the switch (11) “PRO” in the screen will appear: “PRINTING”, and it will give
us an inform of the last sterilization without data, as the following example:
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WARNING MESSAGES
These messages will appear in the second line of the microprocessor screen. They
only appear if there is a sterilization process going on.
PRINTING
This message will appear up to the end of the printing process.
(Only for autoclaves with IT printer)
FAILURE MESSAGES
The failures detected during the sterilization process, a part from giving you the
printing warning, are accompanied with the sound of an acoustic signal and the light
of LCD. Pressing the switch “START/STOP”, the sound stops. To delete an alarm
message; maintain pressed the button “START/STOP” during 2 seconds; until the
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message is deleted, in case you don’t have to do a discharge phase, if the cycle was
in a determined phase, the control still consider to be pressure inside the autoclave,
or the temperature is still high, in the case of sterilizing liquids when the cooling
process is finished or the discharge, the acoustic signal will sound.
FAULT PROBE 1
The control detects that the temperature probe Pt-100 is broken. It is impossible to
proceed with the sterilization. If during the sterilization the probe is broken the
process stops.
FAULT HEATING
When a cycle of sterilization starts, the evolution of the temperature increase is being
controlled up to the temperature selected. If the temperature does not evolute
correctly, that means there must be a problem and the cycle is aborted.
FAULT TEMPERATURE
During the sterilization process the temperature must be between a scale of value to
guarantee a correct sterilization., if this value is modified more than the setting one,
the sterilization is not correct and the process is aborted.
ELECTRO CUTTING
By the way it is not a failure but an interruption of the sterilization cycle, because of
an electrical cut. This will mean an abortion of the cycle.
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BEWARE !
R. Espinar, S.L. will not accept non-cleaned and non-disinfected apparatus, as
well as apparatus with non-emptied tanks (sterilization chamber, dirty water
tank according to the model) for repairing.
GUARANTEE
This apparatus is guaranteed for a one year period, against any fabrication
failure or defective part. The guarantee does not cover any casualty produced
by improper use of the apparatus or alien casualties to R.ESPINAR, S.L.
If the apparatus is manipulated by non-authorized personnel by R.ESPINAR,
S.L., this guarantee is automatically expired.
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R. Espinar S.L.
Av. del Vallès 322 P.I. Els Bellots * 08227 TERRASSA (Barcelona)
Tel: +34 93.783.03.44 +34 93.783.07.20 * FAX: +34 93.731.37.17
Web site: http://www.raypa.com E-mail: [email protected]
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