Lbybi13 N01a Cristobal HW#1 (Guide Questions)
Lbybi13 N01a Cristobal HW#1 (Guide Questions)
Lbybi13 N01a Cristobal HW#1 (Guide Questions)
LBYBI13 (N01A)
3. What is the relationship of wavelength and numerical aperture in getting a good resolving
power?
In theory, since shorter wavelengths yield to a higher resolution (University of
Cambridge, n.d.), the basis of resolving power, numerical aperture determines the
resolving power of an objective, but the total resolution of a microscope system is also
dependent upon the numerical aperture of the substage condenser. Although other factors
might come into play like the correct alignment, right objective lenses, or the specimen
being stained, considering wavelength and numerical aperture, the higher the numerical
aperture of the total system, the better the resolution. (Abramowitz & Davidson, n.d.)