An Induced Pluripotent Stem Cell Line GZHMCi004 A Derived Fro - 2021 - Stem Ce
An Induced Pluripotent Stem Cell Line GZHMCi004 A Derived Fro - 2021 - Stem Ce
An Induced Pluripotent Stem Cell Line GZHMCi004 A Derived Fro - 2021 - Stem Ce
A B S T R A C T
Achondroplasia (ACH; MIM #100800) is an autosomal dominant genetic disease caused by gain-of-function mutations in FGFR3 gene and results in short-limb
dwarfism. Here, we generated an induced pluripotent stem cell line GZHMCi004-A derived from umbilical cord blood mononuclear cells (UCBMCs) of a fetus
with heterozygous G380R mutation in FGFR3 gene. This iPSC line is a valuable in vitro model to study the pathological mechanism and the treatment of ACH.
(continued )
Resource Table:
Unique stem cell line GZHMCi004-A Unique stem cell line GZHMCi004-A
identifier identifier
* Corresponding authors.
E-mail addresses: linsm@hku-szh.org (S.M. Lin), drchenmin2003@yahoo.com.hk, edchen99@gmail.com (M. Chen).
1
The first three authors contributed equally to this work.
https://doi.org/10.1016/j.scr.2021.102322
Received 4 March 2021; Accepted 27 March 2021
Available online 5 April 2021
1873-5061/© 2021 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license
(http://creativecommons.org/licenses/by-nc-nd/4.0/).
N. Li et al. Stem Cell Research 53 (2021) 102322
2
N. Li et al. Stem Cell Research 53 (2021) 102322
room temperature. Both primary and secondary antibodies used in 3.9. Mycoplasma contamination test
Table 2 were diluted with 5% donkey serum in PBS. All images were
captured using DMi8 fluorescence microscope (Leica). Mycoplasma contamination was tested by PCR using GMyc-PCR
mycoplasma detection kit (Yeasen Biotech Co., Ltd.) according to the
3.7. In vitro directed differentiation manufacture’s instruction.
3
N. Li et al. Stem Cell Research 53 (2021) 102322
Table 2
Reagents details.
Antibodies used for immunocytochemistry/flow-citometry
Pluripotency Markers Mouse anti-OCT4 1:500 Santa Cruz Biotechnology Cat# sc-
5279, RRID:AB_628051
Goat anti-SOX2 1:500 R and D Systems Cat# AF2018, RRID:
AB_355110
Rabbit anti-NANOG 1:100 Abcam Cat# ab80892, RRID:
AB_2150114
Mouse anti-TRA-1–60 1:2000 Millipore Cat# MAB4360, RRID:
AB_2119183
Secondary antibodies Alexa Fluor 488 donkey anti-mouse IgG 1:1000 ThermoFisher Scientific Cat# A-21202,
RRID:AB_141607
Alexa Fluor 594 donkey anti-goat IgG 1:1000 ThermoFisher Scientific Cat# A-11058,
RRID: AB_2534105
Goat anti-mouse IgM (Heavy chain) Cross-Adsorbed 1:1000 ThermoFisher Scientific Cat# A-21042,
Secondary Antibody, Alexa Fluor 488 RRID:AB_141357
Alexa Fluor 488 donkey anti-rabbit IgG 1:1000 ThermoFisher Scientific Cat# A-
21,206, RRID:AB_2,535,792
Alexa Fluor 594 donkey anti-rabbit IgG 1:1000 ThermoFisher Scientific Cat# A-21207,
RRID: AB_141637
Primers
Acknowledgements References
This study was supported by the National Key Research and Devel Foldynova-Trantirkova, S., Wilcox, W.R., Krejci, P., 2012. Sixteen years and counting:
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SZH201902017), P.R. China. We acknowledge the patient and her par Horton, W.A., Hall, J.G., Hecht, J.T., 2007. Achondroplasia. The Lancet 370 (9582),
162–172. https://doi.org/10.1016/S0140-6736(07)61090-3.
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