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    Activity 4 - Hydrolysis of Carbohydrates

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    My Roses Are Rosè
    1. The document is a lab report on the hydrolysis of carbohydrates, specifically starch and sucrose. It tests the effects of heat, water, and room temperature on the compounds. 2. For starch, tube A with hydrochloric acid and heat showed a positive Benedict's test and negative iodine test, indicating hydrolysis to glucose. 3. For sucrose, tube A with hydrochloric acid and heat showed positive results for Benedict's and Seliwanoff's tests, indicating hydrolysis to glucose and fructose.

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    Activity 4 - Hydrolysis of Carbohydrates

    Uploaded by

    My Roses Are Rosè
    143 views2 pages
    1. The document is a lab report on the hydrolysis of carbohydrates, specifically starch and sucrose. It tests the effects of heat, water, and room temperature on the compounds. 2. For starch, tube A with hydrochloric acid and heat showed a positive Benedict's test and negative iodine test, indicating hydrolysis to glucose. 3. For sucrose, tube A with hydrochloric acid and heat showed positive results for Benedict's and Seliwanoff's tests, indicating hydrolysis to glucose and fructose.

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    1. The document is a lab report on the hydrolysis of carbohydrates, specifically starch and sucrose. It tests the effects of heat, water, and room temperature on the compounds. 2. For starch, tube A with hydrochloric acid and heat showed a positive Benedict's test and negative iodine test, indicating hydrolysis to glucose. 3. For sucrose, tube A with hydrochloric acid and heat showed positive results for Benedict's and Seliwanoff's tests, indicating hydrolysis to glucose and fructose.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    143 views2 pages

    Activity 4 - Hydrolysis of Carbohydrates

    Uploaded by

    My Roses Are Rosè
    1. The document is a lab report on the hydrolysis of carbohydrates, specifically starch and sucrose. It tests the effects of heat, water, and room temperature on the compounds. 2. For starch, tube A with hydrochloric acid and heat showed a positive Benedict's test and negative iodine test, indicating hydrolysis to glucose. 3. For sucrose, tube A with hydrochloric acid and heat showed positive results for Benedict's and Seliwanoff's tests, indicating hydrolysis to glucose and fructose.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 2

    NAME: _____________________________ Date: ________ SCORE: __________

    Lab. teacher: ___________________ Course & Section_________ Grp. No.______

    Activity No. 4
    HYDROLYSIS OF CARBOHYDRATES

    A. STARCH

    TUBE Treatment Benedict’s Iodine


    Red precipitate was Yellowish Color was
    A HCl, heat observed (positive) observed (negative)

    Blue color remains Blue-black color was


    B H2O, heat (negative) observed (positive)

    Blue color remains Blue-black color was


    C RT, H2O (negative) observed (positive)

    B. SUCROSE

    TUBE Treatment Benedict’s Seliwanoff’s

    Orange Red precipitate was Brick-red precipitate was


    A HCl, heat
    observed (positive) observed (positive)
    Blue color remains Blue color remains
    B H2O, heat
    (negative) (negative)
    Blue color remains Blue color remains
    C RT, H2O
    (negative) (negative)

    Question Guide:
    1. In which tube (A, B, C) have you observed hydrolysis of starch and sucrose? Prove
    your answer.
    Starch – In tube A, we observed the hydrolysis process of starch because it has a red
    precipitate - a positive result in Benedict’s test which indicates that the starch is
    converted into glucose and a yellowish color - a negative result in Iodine test which is
    indicates the absence of reducing sugar glucose.

    Sucrose - Tube A also exhibits the hydrolysis process because of positive results in
    Benedict's and Seliwanoff's tests. Sucrose is a non-reducing sugar; therefore, it will
    have a negative effect on both. With a positive result, it is possible to conclude that
    hydrolysis happened, releasing fructose and glucose.
    2. Write the equation involved in the hydrolysis of sucrose.

    Acid

    Heat

    3. Give the stepwise hydrolysis of starch and its reaction to Benedict’s and Iodine test.
    - https://studylib.net/doc/5790906/hydrolysis-of-starch-by-acid

    Iodine test Benedict’s test

    1. Starch Blue Negative

    2. Soluble starch Blue Negative

    3. Amylodextrin Purple Negative

    4. Erythrodextrin Red Positive

    5. Achrodextrin Colorless Positive

    6. Maltose Colorless Positive

    7. Glucose Colorless Positive

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