Harmala alkaloid extraction ( Passiflora Caerulea ) pictorial ! - Harmala... https://www.dmt-nexus.me/forum/default.aspx?

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Welcome to the DMT-Nexus » OTHER ENTHEOGENS » Harmalas » Harmala alkaloid extraction ( Passiflora Caerulea ) pictorial !

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Harmala alkaloid extraction ( Passiflora Caerulea ) pictorial ! Options

Ambivalent #1 Posted : 31/12/2011 20:22:06

As i mentioned, i prepared a pictorial on how the extraction process went ( i was in a bit of rush aand it is my first, so the pictorial is
not of the best quality ) :

I found that if you are working with basic kitchen equipment and not more advanced and more suitable equipment for your method
of extracting, the patience is always THE key to successful and clean extraction.

DMT-Nexus member
Plant Material : 200-300 g fresh plant material of Blue Passiflora (my first assumption was that i used around 400-500 g fresh plant
material, but when we actually measured what we visualy thought is 500g, was more close to 300 g.
Posts: 336
EDIT : The plant i harvested grows in the southern Balkan. The climate here usually varies, the summer's are hot and the winter's
Joined: 01-lug-2011
moderately cold.Last two winters are pretty warm. We had temperatures above zero more than half december, and now january
Last visit: 11-ago-2016
also.Bottom line is, I don't know how variable are these species..especially when they are adapted on a climate which is not native
Location: Gaia
to them. This is something that i will also try to document and confirm.

Acid : Acetic and Phosphoric

Base : NaOH, baking soda

Measurements were made with really accurate PH meter which also compensates on temperature changes.
(I think for these kind of extractions, a good PH meter is a must if you want clean and successful extraction.) Thats why i usually
don't measure volumes of how much acid or base i'm adding.

First i let the plant material to soak in cold water for at least 30 mins, or hour. My goal was to wash of any contaminants that might
be on the leaves and other parts of the plant, because it grows in an urban area where a lot of vehicles pass by every hour.

After that i covered the plant material in water and i acidified with Acetic acid (used around 30 or 40 ml) till the PH meter readed
3.50 , 3.60

The cooking plate was set to slowly cook the plant more like brewing a tea, not really boiling temperature.
After few hours of brewing and occasional mixing i filtered and got around 2 and a half liters of orange liquid.

only a small amount of the brewed tea

The tea was left on cold temperature overnight and the next day was filtered once more from the plant material that has
accumulated on the bottom.It was than reduced first on half the volume, than filtered again to keep it as clean as it can be before
basing. Than reduced again at approximately half liter and of course filtered once more.

At the first basing i was using NaOH for base which i powdered before i added it.I was adding veery small amount's and was mixing
slowly to help it dissolve quicker.Total base added was almost equivalent on the acid used in the first acidification, around 3 g . After
each few pinches of base added, the Ph was tested.The precipitation was visible around Ph 7 . I based until Ph 9 . This is the jar after
the basification, but the precipitates are almost all collected, only small amount can be seen on the very bottom.

the jar with the tea after the basification

From here i very simply followed Gibran's TEK for further cleaning and extracting the freebase alks.
This next photo is after one or two washes of the precipitates with slightly basic water.

another photo of washing the precipitates (the color of the water looks cleaner here) , but the precipitates still have darker color !

After i cleaned the basic water sufficiently i decanted the water and i made acidic solution with Phosphoric acid.I then dissolved the
precipitates and left them over night ( this step required some more time and patience ) .
The next day there was some insoluble impurities on the bottom just like on Gibran's Tek . The solution was decanted and then again
based with baking soda adding only enough to precipitate the alkaloids. Carefull measurement is needed not to over do it, less
cleaning steps are needed later if done properly.

the precipitates washed with slightly basic water

second wash of the precipitates

1 di 3 08/09/2016 13:02
Harmala alkaloid extraction ( Passiflora Caerulea ) pictorial ! - Harmala... https://www.dmt-nexus.me/forum/default.aspx?g=posts&t=28445

The third and fourth washes were done with normal tap water.The water in my city is pretty basic ( slightly above Ph 7 ) so i think its
ok.

The water was then decanted as much as possible.The work was done with help of plastic syringe.The drying is the part i find most
time consuming. I was drying with moderately hot air but was afraid not to over do with heat.

This was the final yield which on my scale showed 940 mg . It should be relatively pure, because it gets completely diluted in acidic
water with no or very little residue ( maybe dust or sodium carbonate particles but negligible ).

Thanks to everyone for sharing their resources and experiences, gained by dedication in exploration.

The plant's and the knowledge we gain about our self and others through the experiences they make available for us...These things
amongst other's are the most essential occurrences on this planet in all ages of mankind.

All the best in the New Year to everyone, Much Love !!

The-Nexian.me
Psychedelic news, articles, interviews and art from the DMT-Nexus and other sources.

jamie #2 Posted : 31/12/2011 21:06:21

good work! I would love to see analysis on this stuff though to verify what it actaully is. HCL conversion would be cool to see as well
to see if it would crystalize as a salt. Harmine and harmaline hcl is a greenish brown color, while freebase is off white greysih so if it
ends up yellowish brown/green as HCL it is most likely harmalas.
DMT-Nexus member

make it happen, or don't.

Posts: 12159

Joined: 12-nov-2008

Last visit: 08-set-2016

Location: turtle island

smokerx #3 Posted : 01/01/2012 16:02:21

great work, thanks for that.

We are each of us angels with only one wing, and we can only fly by embracing one another.

ThGiL fO TiRipS
*********

Posts: 1786 We are all living on our own faces.

Joined: 26-feb-2011

Last visit: 17-lug-2016

Location: Earth

Ambivalent #4 Posted : 01/01/2012 21:47:50

Thanks, i couldn't have done it without you guys at the Nexus !

jamie wrote:

good work! I would love to see analysis on this stuff though to verify what it actaully is. HCL conversion would be cool to see as
DMT-Nexus member well to see if it would crystalize as a salt. Harmine and harmaline hcl is a greenish brown color, while freebase is off white
greysih so if it ends up yellowish brown/green as HCL it is most likely harmalas.
Posts: 336

Joined: 01-lug-2011

Last visit: 11-ago-2016 I would also love to confirm my doubts about what exactly i am playing here with. I mean, how possible is it to be something else
Location: Gaia besides beta carbolines ?! What else could precipitate out ?

I did some testing with citric acid on this substance in the meantime. 300 mg were dissolved very fast with only couple of lemon
drops, and after evaporation there was yellow-green layer that was crystal like ( hard - crunchy ) . Will bio - essay this in small
amount's and i will post my finding's

jamie #5 Posted : 10/04/2012 16:29:23

any updates on the bioassay?

make it happen, or don't.

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