Mil PRF 25567e
Mil PRF 25567e
Mil PRF 25567e
com
METRIC
MIL-PRF-25567E
17 November 1998
SUPERSEDING
MIL-L-25567D
15 June 1983
PERFORMANCE SPECIFICATION
This specification is approved for use by all Departments and Agencies of the Department of
Defense.
1. SCOPE
1.1 Scope. This specification covers two types of a gas leak detection compound that is
compatible with oxygen. The leak detection compound is intended for use in detecting leaks
in both high- and low-pressure oxygen systems in aircraft and other related oxygen systems.
1.2 Classification. The compound consists of the following types, as specified (see 6.2).
2. APPLICABLE DOCUMENTS
2.1 General. The documents listed in this section are specified in sections 3 and 4 of this
specification. This section does not include documents cited in other sections of this specification
or recommended for additional information or as examples. While every effort has been made to
ensure the completeness of this list, document users are cautioned that they must meet all
specified requirements documents cited in sections 3 and 4 of this specification, whether or not
they are listed.
Beneficial comments (recommendations, additions, deletions) and any pertinent data which
may be used in improving this document should be addressed to: Defense Supply Center
Richmond (DSCR), ATTN: DSCR-VBD, 8000 Jefferson Davis Highway, Richmond, VA
23297-5610 by using the Standardization Document Improvement Proposal (DD Form 1426)
appearing at the end of this document or by letter.
AMSC N/A FSC 6850
DISTRIBUTION STATEMENT A. Approved for public release; distribution is unlimited.
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2.3 Non-government publications. The following documents form a part of this document to the
extent specified herein. Unless otherwise specified, the issues of the documents which are DoD
adopted are those listed in the issue of the DoDISS cited in the solicitation. Unless otherwise
specified, the issues of documents not listed in the DoDISS are the issues of the documents cited
in the solicitation (see 6.2).
ASTM B152 Copper Sheet, Strip, Plate, and Rolled Bar (DoD adopted)
ASTM B209-96 Aluminum and Aluminum-Alloy Sheet and Plate (DoD adopted)
ASTM D92 Test for Flash and Fire Points by Cleveland Open Cup
(DoD adopted)
ASTM D1173 Test for Foaming Properties of Surface-Active Agents
ASTM D1177 Test for Freezing Point of Aqueous Engine Anti-freeze Solutions
(DoD adopted)
ASTM D1292 Test for Odor in Water
(Application for copies should be addressed to the American Society for Testing and
Materials, 100 Barr Harbor Drive, W. Conshohocken, PA 19428-2959.)
(Application for copies should be addressed to the SAE International, 400 Commonwealth
Drive, Warrendale, PA 15096-0001.)
2.4 Order of precedence. In the event of a conflict between the text of this document and the
references cited herein, the text of this document takes precedence. Nothing in this document,
however, supersedes applicable laws and regulations unless a specific exemption has been
obtained.
3. REQUIREMENTS
3.1 First article. When specified (see 6.2), a sample shall be subjected to first article inspection in
accordance with 4.1.1.
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3.2.1 Composition. The compound shall be liquid and miscible with water in all proportions.
3.2.1.1 Foreign matter. The compound shall be free from any sediment or suspended matter.
3.2.1.2 Ingredients not allowed. The compound shall not contain (a) mineral oil, vegetable oil,
animal oil, or fats or (b) ketones, aldehydes, or alcohols as components in the formulation.
3.2.1.3 Igniting material. The compound shall not contain any material that will ignite or explode
when in contact with liquid or gaseous oxygen.
3.2.1.4 Skin irritant material. The compound shall not contain any material that will act as
primary skin irritants, skin sensitizers, or produce any other dermatosis.
3.2.2 Odor. The compound shall not possess or produce any objectionable odors. The threshold
odor number will be a maximum of 1 with a maximum odor intensity index of 0.
3.2.3 pH Value. The compound shall have a pH value between 6.0 and 7.5 at 21 degrees C.
3.2.4 Nonflammability. The compound shall not support combustion below 100 degrees C.
3.2.6 Foaming ability. The compound shall be able to produce an initial foam height of not less
than 145 mm and a foam height of 130 mm after standing for 5 minutes.
3.2.7 Freezing point. Type II compound shall have a freezing point of -60 degrees C or below.
3.2.8 Residue. The compound shall not contain more than 0.50 percent total residue.
3.2.10 Compatibility with oxygen. The compound shall be compatible with oxygen.
3.2.11 Mold growth. The solution shall not support mold growth within the container during use
or storage.
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4. VERIFICATION
4.1 Classification of inspections. The inspection requirements specified herein are classified as
first article inspection (see 4.1.1) and conformance inspection (see 4.1.2).
4.1.1 First article inspection. Perform a first article inspection on the initial production-
representative unit of an order or when required by the contract. When a first article inspection is
required (see 6.2 and 6.3), it includes all verifications listed in table I.
4.2 Verification methods. Acceptable verification methods included in this section are visual
inspection, measurement, sample tests, full-scale demonstration tests, simulation, modeling,
engineering evaluation, component properties analysis, and similarity to previously-approved or
previously-qualified designs.
4.2.1 Verification alternatives. The manufacturer may propose alternative test methods,
techniques, or equipment, including the application of statistical process control, tool control, or
cost-effective sampling procedures to verify performance. See the contract for alternatives that
replace verifications required by this specification.
4.2.2.1 Foreign matter inspection. The compound shall be examined visually to determine if it is
clear and free from suspended matter and sediment.
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4.2.2.2 Odor test. Odor shall be tested in accordance with ASTM D1292.
4.2.2.3 pH value verification. The compound shall be tested with an electric pH meter that has
been buffered with two buffer solutions in the desired pH range and is capable of measuring pH to
0.1 point.
4.2.2.4 Nonflammability test. Nonflammability will be tested in accordance with ASTM D92.
4.2.2.5 Leak detection verification. Mount a laboratory screw hose clamp so that when the end
of a rubber or plastic tube of 0.3175 cm is inserted, flush with the clamp jaws; that it will point
upward at an angle of 45 degrees. Attach the other end of the tube to a source of compressed
oxygen gas provided with a suitable gauge. Apply a pressure of 34 KPa and adjust the lead until,
on application of the leak test compound, only slight foaming is observed. Immerse the leak in a
shallow vessel containing the compound, and collect the escaping gas in an inverted glass tube
marked in 0.1 cc divisions filled with the compound. Remove the leak from the bath and apply
the leak detection compound. Immediate foaming shall be observed.
4.2.2.6 Foaming ability test. The compound shall be tested in accordance with
ASTM Method D1173 with the temperature at 49 degrees C.
4.2.2.7 Freezing point test. Type II only shall be tested in accordance with ASTM D1177.
4.2.2.8 Residue verification. Pipet 10 ml of the compound into a clean, dry 50 ml beaker
pre-weighed to the nearest 0.1 mg. Reweigh the beaker plus compound and then heat the
solution to approximately 95 degrees C on a hot plate. Allow 8 ml of the solution to evaporate;
then place the beaker with the remaining solution in an oven at 110 + /- 2 degrees C and dry to
constant weight. The weight of the residue shall not exceed 0.50 percent of the weight of the
original 10 ml of compound.
4.2.2.9.1 Surfaces. Two test panels (2.54 x 15.24 cm each) shall be made from each of the
metals specified in table II. Each test panel will be acetone-washed in a beaker and allowed to air
dry for two hours in a desiccator. Each previously cleaned test panel shall be coated with a 0.1 ml
of compound over 50 percent of the surface area and the panel dried under an infrared reflector
drying lamp. The reflector apparatus shall be so adjusted that a 250-watt drying lamp shall be
15 cm above the test panel. After drying, the panel shall be removed and rinsed under a stream of
cold deionized water. The panels shall rinse cleanly, leaving a bright shiny surface with no residue
or film noticeable by a visual examination.
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4.2.2.9.2 Between the faying edges. Two cleaned test panels (2.54 x 15.24 cm each) from each
of the metals specified in table II, shall be clamped with a suitable clamp. Place 0.1 ml of the leak
detection compound at the faying edge. After one minute, an additional 0.1 ml of the leak
detection compound shall be placed at the faying edge. The panels shall then be rinsed with cold
deionized water and allowed to air dry for 2 hours at 25 degrees C. Then the panels shall be
separated and inspected for any evidence of corrosion at the faying edge. A second set of control
panels shall be used in the same manner as the test panels except that no leak test compound will
be applied. The test panels shall not have any greater degree of corrosion than the control panels
test results.
Metals Surface
Aluminum Alloy Alclad (2024) conforming to -----------------
ASTM B209-96 using Temper = 0
Aluminum Alloy (2024) conforming to Anodized, in accordance with
ASTM B209-96 using Temper = 0 SAE-AMS2471D
Steel, conforming to SAE-AMS5046A-90 Cadmium plated in accordance
(using SAE 1020 and 1025) with SAE-AMS2400S
Copper, conforming to ASTM B152 Annealed
4.2.2.10 Compatibility with oxygen verification. Under ambient conditions, place 100 ml of the
compound into two each 200 ml Dewar flasks. Place a thermometer graduated in 0.1 degrees C
in each flask and record the temperature. Bubble a stream of oxygen from a glass tube through
one of the solutions at 100 +/- 10 ml per minute. Bubble a stream of air at the same rate through
the other solution. Continue for 30 +/- 2 minutes. Observe any temperature rise. The leak
detection compound through which the oxygen was bubbled shall not show any steady or sudden
rise in temperature and the final temperature shall not exceed by 0.5 degrees C the final
temperature of the compound through which the air was bubbled.
4.2.2.11 Mold growth test. Perform the test in Appendix A. There shall not be any mold growth
viewed on the test samples with the leak detection compound. If any mold growth is seen, then
the lot will be immediately rejected.
5. PACKAGING
5.1 Packaging. For acquisition purposes, the contract or order shall specify packaging
requirements (see 6.2). When DoD personnel perform materiel packaging, those personnel need
to contact the responsible packaging activity to ascertain requisite packaging requirements. The
inventory control point packaging activity within the Military Department of Defense Agency, or
within the Military Department’s System Command, maintains packaging requirements.
Packaging data retrieval is available from the managing Military Department’s or Defense
Agency’s automated packaging files, CD-ROM products, or by contacting the responsible
packaging activity.
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6. NOTES
(This section contains general or explanatory information that may be helpful, but is not
mandatory.)
6.1 Intended use. The leak detection compound covered by this specification is military unique.
This specification is the industry standard for this compound. No commercial equivalent exists.
This compound assures the purity of military aviators' breathing systems by prohibiting the
introduction of contaminants. This assures the safety, health, and compatibility of oxygen gas
with the variety of methods used in military aircraft. It also assures the stability of breathing
systems during military operations (bombing, air-to-air combat evasive maneuvering, etc.). These
extreme conditions are not found in commercial applications.
a. Title, number, and date of this specification and any amendments thereto.
b. Type of compound (see 1.2).
c. Size container required as applicable.
d. Issue of DoDISS to be cited in the solicitation, and if required, the specific issue of
individual documents referenced (see 2.3).
e. When first article is required (see 3.1, 4.1.1 and 6.3).
f. Instructions on submittal of first article samples and test reports will be cited in the
contract.
g. Packaging requirements (see 5.1).
6.3 First article. When requiring a first article inspection, the contracting officer should provide
specific guidance to offerors whether the first article is a first article sample, a first production
item, or a number of items to be tested. The contracting officer should also include specific
instructions in acquisition documents regarding arrangements for examinations, approval of first
article test results and disposition of first articles. Pre-solicitation documents should provide the
Government with the waiver rights for samples for first article inspection to bidders offering a
previously acquired or tested product. Bidders offering such products, who wish to rely on such
production or test, must furnish evidence with the bid, that prior government approval is
appropriate for the pending contract.
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6.5 Material safety data sheets. Contractor should furnish a material safety data sheet to the
procuring activity using FED-STD-313.
Corrosion
Fungi
Ingredients
Mold growth
6.7 Changes from previous issue. Marginal notations are not used in this revision to identify
changes with respect to the previous issue due to the extent of the changes.
Reviewers:
Air Force - 11
Army - EA, MI
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APPENDIX A
MOLD GROWTH TEST
A.1 SCOPE
A.1.1 Scope. This appendix details the procedure for conducting a mold growth test. This
appendix is a mandatory part of the specification.
A.3 MOLD RETARDANT. The formulation of the mold retardant used shall be certified as to
its nomenclature, effect in an industrial environment and the non-detrimental effect on the
compounds intended use.
A.4 PROCEDURE
A.4.1 Preparation of mineral salts solution. Using a clean apparatus, prepare a mineral salts
solution containing the following, then verify the pH of the mineral salts solution is between 6.0
and 6.5.
A.4.2 Preparation of mixed spore suspension. Prepare a spore suspension using asceptic
techniques containing the following test fungi, listed in table A-I.
PRECAUTIONS: Although the fungi specified for this test are not normally considered to
present a serious hazard to humans, certain people may develop allergies or other reactions. It is
therefore recommended that standard operation procedures (SOPs) for safety be employed. It is
also recommended that the tests be conducted by personnel trained in microbiological techniques.
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APPENDIX A
TABLE A-I. Test fungi.
A.4.2.1 Spore storage. Maintain pure cultures of the test fungi (see table A-I) separately on an
appropriate medium such as potato dextrose agar except that chaetomium globosum shall be
cultured on strips of filter paper overlayed on the surface of mineral salts agar. Prepare mineral
salts agar by dissolving 15.0 g agar in a liter of the mineral salts solution. Do not keep the stock
cultures for more than 4 months at 6 degrees +/- 4 degrees C; after that time, prepare sub-cultures
and use them for the new stocks. Verify the purity of fungus cultures prior to the mold growth
test.
A.4.2.2 Stock cultures preparation. Incubate subcultures used for preparing new stock cultures
or the spore suspension at 30 degrees +/- 1.4 degrees C for 14 to 21 days. Prepare a spore
suspension of each of the five fungi by pouring into one subculture of each fungus, 10 ml of an
aqueous solution containing 0.05 g per liter of a nontoxic wetting agent such as sodium dioctyl
sulfosuccinate or sodium lauryl sulfate.
A.4.2.3 Stock cultures separation. Use a rounded glass rod to gently scrape the surface growth
from the culture of the test organism. Pour the spore charge into a 125 ml capped Erlenmeyer
flask containing 45 ml of water and 50 to 75 solid glass beads, 5 mm diameter. Shake the flask
vigorously to liberate the spores from the fruiting bodies and to break the spore clumps. Filter the
dispersed fungal spore suspension into a flask through a 6 mm layer of glass wool contained in a
glass funnel. This process should remove large mycelial fragments and clumps of agar.
A.4.2.4 Stock cultures solution. Centrifuge the filtered spore suspension and discard the
supernatant liquid. Resuspend the residue in 50 ml of water and centrifuge. Wash the spores
obtained from each of the fungi in this manner three times. Dilute the final washed residue
with mineral-salts solution in such a manner that the resultant spore suspension shall contain
1,000,000 +/- 200,000 spores per as determined with a counting chamber. Repeat this operation
for each organism used in the test.
A.4.2.5.1 Viability of spore suspension. Prior to preparing the composite spore suspension,
inoculate sterile potato agar plates with 0.2 to 0.3 ml of the spore suspension of each of the
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APPENDIX A
individual fungal species using separate potato dextrose agar plates for each species. Distribute
the inoculum over the entire surface of the plate. Incubate the inoculated potato dextrose agar
plate at 24 degrees to 31 degrees C for 7 days. After the incubation period, check the fungal
growth. The absense of copious growth of any of the test organisms over the entire surface in
each container will invalidate the results of any tests using these spores.
After dipping, remove the excess liquid from the strips and hang them to dry before placing them
in the chamber and inoculating them. Within the chamber, place the strips vertically in close
proximity to and bracketing the test item so that the test strips and test items experience the same
test environment. The length of the strips shall be at least the height of the test item. These strips
verify conditions to promote fungal growth in the chamber.
A.4.2.6 Fungus test suspension. Blend equal volumes of the resultant spore suspension to obtain
the final mixed spore suspension. The spore suspension may be prepared fresh. If not freshly
prepared, it should be held at 6 +/- 4 degrees C for not more than 7 days.
A.4.3 Mold growth test. Complete the mold growth testing in A.4.3.
A.4.3.1 Preparation of incubation. Assure that the condition of the leak detection compound
subjected to testing is similar to that as delivered by the manufacturer or customer for use, or as
otherwise specified. Place 150 ml of the compound in a 250 ml beaker in the chamber or cabinet
on suitable fixtures. Hold the test compound in the operating chamber for at least 4 hours
immediately prior to inoculation. Inoculate the test compound and cotton fabric chamber control
items with the mixed fungal spore suspension by spraying it on the control and on the test sample
in the form of a fine mist from an atomizer or nebulizer. Care should be taken to cover all
external and internal surfaces which are exposed during use or maintenance. Replace covers of
the test items loosely. Start incubation immediately following the inoculation.
A.4.3.2 Incubation of the test compound. Incubate the test sample under a daily cycle of
temperature and humidity conditions consisting of 20 hours of a relative humidity of 95 +/- 5
percent and an air temperature of 30 degrees +/- 1 degree C followed by a 4-hour period.
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APPENDIX A
The 4-hour period consists of 2 hours in which conditions of 95 +/- 5 percent relative humidity
and temperature of 25 degrees +/- 1 degree C are maintained and the other 2 hours of the 4-hour
period is used for the transition(s) of temperature and relative humidity. Temperature and
humidity conditions during the transition periods must be as follows: temperature = 24 degrees to
21 degrees C and relative humidity = above 90 percent.
A.4.3.3 Test duration. Repeat the 24-hour daily cycle for the test duration. After 7 days, inspect
the growth on the control cotton strips to assure that the environmental conditions in the chamber
are suitable for growth. For this assurance, at least 90 percent of the part of the surface area of
each test strip located at the level of the test item should be covered by fungi when inspected
visually. If not, repeat the entire test with the required adjustments of the chamber to produce
conditions suitable for growth. Leave the control strips in the chamber for the duration of the
test; note their condition at this time and record it. If the cotton strips show satisfactory fungus
growth after 7 days, continue the test for 72 hours from the time of inoculation. If there is a
decrease in fungal growth on the cotton strips at the end of the test as compared to the 7-day
results, the test is invalid.
A.4.4 Inspection. At the end of the incubation period, inspect the test samples immediately. If
possible, inspect the samples within the chamber. If the inspection is conducted outside of the
chamber and not completed in 8 hours, return the test samples to the test chamber or similar
humid environment for a minimum of 12 hours. There shall not be any mold growth viewed on
the test samples with the leak detection compound. If any mold growth is seen, then the lot will
be immediately rejected.
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3. The preparing activity must provide a reply within 30 days from receipt of the form.
NOTE: This form may not be used to request copies of documents, nor to request waivers, or clarification of requirements on current
contracts. Comments submitted on this form do not constitute or imply authorization to waive any portion of the referenced
document(s) or to amend contractual requirements.
1. DOCUMENT NUMBER 2. DOCUMENTDATE (YYMMDD)
I RECOMMEND A CHANGE: MIL-PRF-25567E 17 NOVEMBER 1998
3. DOCUMENT TITLE LEAK DETECTION COMPOUND, OXYGEN SYSTEMS
4. NATURE OF CHANGE
(Identify paragraph number and include proposed rewrite, if possible. Attach extra sheets as needed.)
6. SUBMITTER
a. NAME (Last, First, Middle Initial) b. ORGANIZATION
c. ADDRESS (Include Zip Code) d. TELEPHONE (Include Area Code) 7.DATE SUBMITTED
(1) Commercial (YYMMDD)
(2) AUTOVON
(if applicable)
8. PREPARING ACTIVITY
a. NAME DEFENSE SUPPLY CENTER RICHMOND b. TELEPHONE Include Area Code)
(1) Commercial (2) AUTOVON
c. ADDRESS (Include Zip Code) IF YOU DO NOT RECEIVE A REPLY WITHIN 45 DAYS, CONTACT:
ATTN: DSCR-VBD DEFENSE QUALITY AND STANDARDIZATION OFFICE
8000 JEFFERSON DAVIS HIGHWAY 5203 Leesburg Pike, Suite 1403, Falls Church, VA 22401-3466
Telephone (703) 756-2340 AUTOVON 289-2340
RICHMOND, VA 23297-5610
DD Form 1426, OCT 89 Previous editions are obsolete. 198/290