Water Quality - Determination of Total Nitrogen - Alkaline Potassium Persulfate Digestion UV Spectrophotometric Method
Water Quality - Determination of Total Nitrogen - Alkaline Potassium Persulfate Digestion UV Spectrophotometric Method
Water Quality - Determination of Total Nitrogen - Alkaline Potassium Persulfate Digestion UV Spectrophotometric Method
HJ
ENVIRONMENTAL PROTECTION STANDARD
HJ 636-2012
Replacing GB 11894-89
碱性过硫酸钾消解紫外分光光度法
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HJ 636-2012
ANNOUNCEMENT
Ministry of Environmental Protection of PRC
No. 16, 2012
It hereby announces.
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HJ 636-2012
Table of Contents
ANNOUNCEMENT Ministry of Environmental Protection of PRC .................... 2
Foreword .......................................................................................................... 4
7 Apparatus ................................................................................................... 8
8 Sample ....................................................................................................... 9
9 Sample ....................................................................................................... 9
13 Notices ................................................................................................... 12
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HJ 636-2012
Foreword
This Standard was issued in 1989 for the first time. The original drafting
organization was Shanghai Environmental Monitoring Center. This is the first
revision. The main content of revision is as follows:
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HJ 636-2012
1 Application scope
When the amount of sample is 10 ml, the detection limit of this method shall be
0.05 mg/L; the measuring range shall be 0.20 ~ 7.00 mg/L.
2 Normative references
The following standards contain the provisions which, through reference in this
Standard, constitute the provisions of this Standard. For undated reference
documents, the latest versions apply to this Standard.
For the purposes of this document, the following terms and definitions apply.
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HJ 636-2012
Weigh 20.0 g of sodium hydroxide (6.2) and make it dissolved in a small amount
of water. Dilute to 100 ml.
Weigh 10.0 ml of sodium hydroxide solution (6.9). Use water to dilute to 100 ml.
7 Apparatus
7.2 High-pressure steam sterilizer: the maximum working pressure shall not
be less than 1.1 ~ 1.4 kg/cm2; the maximum working temperature shall not be
less than 120 ~ 124°C.
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HJ 636-2012
9.2 Determination
NOTE 2 When the nitrogen content in the sample exceeds 70 µg, it may reduce the
sampling amount and add water to dilute it to 10.00 ml.
Where,
ρ - total nitrogen (in N) mass concentration in sample, in mg/L;
Ar - difference between corrected absorbance of sample and corrected absorbance of
blank test;
a - intercept of calibration curve;
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HJ 636-2012
total nitrogen content of sample is > 1.00 mg/L, the relative deviation of the
measured results shall be ≤ 5%. The results shall be reported in the average of
two parallel samples.
12.5 At least 10% of the spiked sample should be determined for each batch
of sample. When the number of sample is less than 10, it shall at least
determine a spiked sample. Spike recovery should be between 90% ~ 110%.
13 Notices
13.3 The vessel used in the experiment and the high pressure steam sterilizer
should be free of nitrogen pollution. The glassware used in the experiment
should be soaked in hydrochloric acid solution (6.7) or sulfuric acid solution
(6.8). Rinse with tap water and then rinse with ammonia-free water for several
times. Use immediately after washing. Autoclaves should be cleaned weekly.
13.5 When using high pressure steam sterilizer, the pressure gauge should
be checked regularly. And it should check the seal of rubber seal so as to avoid
leakage caused by decompression.
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HJ 636-2012
Weigh 0.35 g of hydrazine sulfate and make it dissolved in water. Dilute to 500
ml. Prepare it when required.
Add 25.0 ml of sodium hydroxide test solution (A.2.11) and 40.0 ml of water in
125 ml digestion bottle.
In 125 ml digestion bottle, add 25.0 ml of sodium hydroxide test solution (A.2.11),
30.0 ml of water and 10.00 ml of nitrogen standard solution (A.2.10).
In 125 ml digestion bottle, add 5.0 ml of sodium hydroxide test solution (A.2.11)
and 60.0 ml of water.
Weigh 9.2 g of potassium persulfate and make it dissolved in water. Set volume
to 200 ml.
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