Sensacore ST200 User Manual

ST-200 Plus
Electrolyte Analyzer
OPERATOR’S
MANUAL (5 Channel, Unislider, Welco Pump)
RELIABL
ASY TO USE
ASY TO AFFORD
ST-200 PLUS (5 Ch) Operator’s Manual
ST-200 PLUS (5 Ch)

Na/K/iCa/Li/pH/Cl Electrolyte Analyzer
OPERATOR’S MANUAL
SENSA CORE MEDICAL INSTRUMENTATION PVT. LTD.

Plot No.3, Export Promotion Industrial Park,
Pashamylaram, Medak(Dist), Hyderabad, Telangana, 502307.
www.sensacore.com, Tel: +08455 223400.
[email protected]
1
PREFACE
Understanding the ST-200 PLUS (5 Ch) Electrolyte

1. ….. 5
Analyzer
A. Intended Use ….. 5
B. Summary and Explanation ….. 5
C. Operational Hazards and Precautions ….. 6
2. Analyzer Installation ….. 8
A. Unpacking ….. 8
B. Component Installation/Replacement ….. 8
a. Valve Block ….. 8
b. Sample Probe ….. 9
c. Reference Electrode ….. 10
d. Building the Electrodes stack ….. 11
e. Installation of tubing ….. 12
f. Reagent Pack Installation ….. 15
C. Analyzer Restoration ….. 16
D. Printer Setup ….. 17
E. Power Up ….. 18
3. Operating the Analyzer ….. 20
A. Calibration ….. 20
B. Analyze Sample ….. 22
C. Daily Cleaner ….. 25
D. Standby ….. 30
E. Sub Menu ….. 31
F. Analyze Urine ….. 34
G. Stored Results ….. 37
H. Reagent Pack Consumption ….. 38
I. Quality Control ….. 38
J. Operator Functions ….. 39
a. Diagnostics ….. 39
b. Users menu ….. 52
2
c. Setup menu ….. 61

d. Parameters ….. 66
e. Select iCa Unit ….. 67
4. Sample Handling and Collection ….. 68
A. Whole Blood ….. 68
B. Serum ….. 68
C. Plasma ….. 69
D. Urine ….. 69
E. Expected Values ….. 70
5. Maintenance ….. 71
6. Principles of Operation ….. 72
A. ISE Theory ….. 72
7. Specifications of Na/K/iCa/Li/pH/Cl ….. 74
8. Troubleshooting ….. 76
Introduction ….. 76
A. Flow path system ….. 78
B. Flow error ….. 81
C. Electrode Assembly ….. 85
D. Reagent Pack ….. 87
E. Bubble Detector ….. 87
F. Printer ….. 87
9. Display Messages ….. 89
10. Appendices ….. 94
Warranty ….. 94
3
FIGURES
S/N Figure Figure no. Page no.

1. ST-200 PLUS (5 Ch) 1 7
2. Valve module 2 8
3. Removing sample probe 3 9
4. Fixing sample probe 4 9
5. Reference electrode 5 10
6. Reference electrode removal 6 10
7. Building the Electrode Stack 7 12
8. Pump tube installation 8 14
9. Reagent pack installation 9 15
10. Printer setup 10 18
11. Back view connection 11 19
12. Aspirating sample 12 22
13. Aspirating daily Cleaner 13 25
14. Pressing standby button 14 29
15. Aspirating urine 15 35
16. Slope Factor 16 59
17. Intercept 17 60
18. Flow path system 18 78
4
ST-200 PLUS (5 Ch) ELECTROLYTE ANALYZER
1. Understanding the ST-200 PLUS (5 Ch) Electrolyte Analyzer
A. Intended Use
The ST-200 PLUS (5 Ch) is an automated, microprocessor-controlled

analyzer for measurement of sodium, potassium, ionized calcium, Lithium,
Chloride & pH in serum, plasma, whole blood, diluted urine and
cerebrospinal fluid.
To obtain accurate results, the ST-200 PLUS (5 Ch) must be operated with
Sensacore’s specially packaged calibrant and bovine-based control
materials and be maintained as described in this manual.
B. Summary and Explanation
Designed for fast, efficient use, the ST-200 PLUS (5 Ch) provides clinically
accurate analysis for patient diagnosis and treatment. The analysis takes
65 seconds and requires approximately 130 micro liters of serum, plasma,
whole blood, CSF or 500 micro liters of diluted urine (80 seconds urine
analysis cycle).
You will be guided through the display menu by pressing on keypad the
“Y” or “N” button in response to questions and messages that appear on
the display. Calibration is automatic, but can be performed on demand. A
unique reagent pack contains Cal “A” & Cal “B” to calibrate the analyzer.
The disposable reagent pack also contains a waste bag for analyzed
samples and solutions, eliminating the need to handle biological waste
materials.
Many medication states the cause of temporary imbalance of the body’s

electrolyte, requires necessary treatment to the patient. Drugs for
hypertension act as diuretics, causing the body to excrete high levels of
potassium in the urine. Imbalances in the body’s potassium level affect the
neurological and muscular activity of the body. Drugs that influence
sodium concentration include all diuretics, chloropropamide, vasopressin,
antihypertensive agents and corticosteroids. Sodium imbalance is often
associated with dehydration and edema. Chloride values are seen in
metabolic acidotic states and in salt losing renal diseases.
5
C. Operational Hazards and Precautions
Read the operator’s manual before setting up or operating the ST-200

PLUS (5 Ch) electrolyte analyzer.
Observe all warnings, notes and key information in this manual.
Failure to leave the ST-200 PLUS (5 Ch) electrolyte analyzer to power with
the REAGENT PACK in place could damage the electrodes and cause
blockage.
There are no operator serviceable parts inside the ST-200 PLUS (5 Ch)
electrolyte analyzer. If electromechanically problems are suspected, DO
NOT attempt to open the back cover. Contact your authorized technical
service representative.
The power cord of the ST-200 PLUS (5 Ch) electrolyte analyzer must be
connected to a matching ground outlet supplying 110 VAC, 50/60 Hz 0r
220 VAC, 50/60 Hz, as indicated on the label on the rear panel of the
analyzer.
The ST-200 PLUS (5 Ch) electrolyte analyzer contains sensitive electronics

and must be properly grounded. The ST-200 PLUS (5 Ch) electrolyte
analyzer should not be plugged into a circuit protected by a GFI
(Ground Fault Interrupter).
The environment should be as free as possible from dust, mechanical

vibrations, and electrical interference. Avoid proximity to brush-type
motors (certain type of centrifuges), diathermy instruments, flickering
fluorescent lights and arcing contacts of any kind. Do not install the ST-
200 PLUS (5 Ch) electrolyte analyzer near heat producing equipment or
near incandescent lighting.
BIOHAZARD
When collecting and handling biological specimens, the center for Disease
control (CDC) recommends that all samples be considered biohazards
which may be contaminated with HIV or other pathogens. Any replaceable
item which comes in contact with biological samples, including the sample
probe, solution valve, probe wipers, sample tube, sample detector,
electrodes, electrode connectors, membrane assembly, electrode housing,
pump tubing and solutions pack may contain potentially contaminated
material. Treat all components, during use and dispose, as you would use
any biohazardous material.
6
ST-200 PLUS (5 Ch):
Figure 1
7
INSTALLATION
2. Analyzer Installation
A. Unpacking
Upon arrival, carefully remove the ST-200 PLUS (5 Ch) electrolyte analyzer
and accessories from shipping containers and place on solid work surface.
Visually inspect ST-200 PLUS (5 Ch) electrolyte analyzer for any damage
sustained during shipment. If any damage is found, please inform to your
nearest authorized dealer of ST-200 PLUS (5 Ch) electrolyte analyzer.
B. Component Installation/Replacement
To install or replace ST-200 PLUS (5 Ch) electrolyte analyzer components,

follow the procedures in this chapter. New ST-200 PLUS (5 Ch) electrolyte
analyzers are shipped with the valve module and sample probe installed.
a. valve module:
Valve module is mounted in ST-200 PLUS (5 Ch) for the supply of

calibrant A & calibrant B solutions as per requirement.
Valve module:
Valve module be made up of two parts, 1st part is permanent & 2nd part is
disposable seal’s as shown in below.
Permanent Part
Disposable Part
Figure 2
8
b. Sample Probe:
First rotate the sample probe until the side probe hole faces backward.
Unscrew the screw on the unislider with help of L - key, Insert sample
probe in the sample probe holder till stopper mark which is present on
Sample Probe. Tighten the screw on the unislider with help of L - key
properly.
Removing sample probe
Figure 3
Probe Removal: unscrew the screw on the unislider with help of L - key
and remove the sample probe.
Fixing sample probe:
Figure 4
9
c. Reference Housing:
Remove the reference electrode from its package. Wipe the outside of the
reference electrode dry. Slide the new reference electrode into the back of
the electrode housing. Make sure all surfaces are clean and dry.
Figure 5
Reference Housing: Electrode Removal
Slide the electrode stack up at least one inch by gently pushing up on the
Cl electrode pin. This will remove reference electrode from or stack
electrodes.
Figure 6
10
d. Building the Electrode Stack:
Remove electrodes, seals and electrode housing from the packaging.

Assemble the electrodes with seals and sample detector in electrode
housing. Make sure each component is clean and dry, and then stag the
electrode by inserting seals in between every electrode make sure that
there should be a seal at the bottom of electrode also. Connect the sample
tube at the top of the bubble detector. Stag the electrodes as shown in fig.
Electrode housing must be in place to ensure proper operation. Upon

replacement of electrodes, use new electrode rubber seal. The seals must
be clean and dry.
MODEL 1 -- K/ Na
MODEL 2 -- K/ Na /Cl
MODEL 3 -- K/ Na /iCa
MODEL 4 -- K/ Na /iCa /pH
MODEL 5 -- K/ Na /iCa /Cl
MODEL 6 -- K/ Na /iCa /Li
MODEL 7 -- K/ Na /iCa /Li/pH
MODEL 8 -- K/ Na /iCa /pH/Cl
11
Figure 7
Loading the Stack
Slide the electrode stack into the electrode housing. Push down firmly
until the sample detector snaps into position.
Carefully install the bubble detector and the six electrodes into the
electrode housing in such a manner that electrode silver pin should be
inserted in female connector.
Do not force the electrode housing into place. If the electrodes are properly
installed, the electrode housing should slide easily into position.
e. Install the Tubing:
Connect the sample tube to the top of the sample probe.
The pump tube has two stoppers. One stopper (red) is connected at the
base of the electrode housing and the other stopper (blue) is connected to
the reagent pack socket i.e. to the waste.
12
Tube installation
Front View Pump Assembly Modules
13
Figure 8
Pump module installation procedure:
1. Module base: It is fixed to the chassis through a lock at the top of

base as shown in the figure. We need to place the base bottom side
first followed by the top, which consists of lock.
2. Rollers: It consists of grove at its bottom and a knob on the front
side. Grove side should be inserted to the base knob.
3. Pump tubing: Insert the tubing as per the direction by holding the
roller firmly as shown in the figure.
4. Front cover: It is used to lock the rollers and pump tubing as
shown in the figure. Ensure that the tag of pump tubing should be
above the front cover lock.
14
f. Reagent Pack Installation:
Do not squeeze the reagent pack. Hold the reagent pack in your left hand
and place reagent pack into front of the analyzer; slide the reagent pack
firmly to right plugging it into the valve module. Push the reagent pack
until it stops.
Do not use expired reagent pack. The electrode should be exposed to

ST-200 PLUS (5 Ch) reagent all time so that it will protect electrode from
getting damaged.
Figure 9
15
C. Analyzer Restoration:
To remove or replace any ST-200 PLUS (5 Ch) components, restore the

ST-200 PLUS (5 Ch) in following order:
 Place the instrument into Maintenance mode.
MENU
------
1.DIAGNOSTICS
2.USER MENU
3.SETUP MENU
4.PARAMETERS
5.SELECT iCa UNIT
Press 1
↓
DIAGNOSTICS
------------
1.DAILY CLEANER
2.MAINTENANCE
3.SENSORS
4.FLOW
5.HARDWARE
6.REAGENT PACK
Press 2
↓
ENTERING MAINTENANCE
Please wait …
Cleared liquid from the tubing

↓
***MAINTENANCE ***
N→ Exit
 To clean the flow path:
1. Remove the electrode housing by disconnecting the sample tube

from the top of the sample Probe and disconnect the pump tube &
sample detector from the electrode housing.
2. Enter into maintenance mode and remove the reagent pack. Then,
connect dark green color needle to syringe followed by a
tygon/transparent tube and aspire it with DI water or daily cleaner.
Now, remove the electrodes stacking from reference housing and
flush the stacking by connecting the other end of syringe to it.
16
 To clean the electrodes:
If the Calibration slopes are out of range. Then, clean the electrodes
by flushing with DI water/CAL A. If required clean electrodes with
spring needles provided in troubleshooting kit.
Caution: Don’t use the spring needle which is already used for Cl
electrode to any other electrodes. And don’t use spring needle
several times to clean the electrodes which may cause damage to
electrode membrane.
D. Printer Setup:
The built-in printer will provide a permanent record of calibration and

analysis results. Other data can also be requested for printing. If required,
the printer can be turned off. (To prevent damage to the printer head
mechanism, the printer must be turned off if the electrolyte analyzer runs
out of printer paper.)
Refer to the following precautions:
In order to open the printer door lift the left side lock upward direction. To
install the roll of paper into the printer, cut off the beginning section of
paper that has tape or adhesives. After installing the paper, close the cover
on the top of the housing until the door gets locked.
Use of the ST-200 PLUS (5 Ch) electrolyte analyzer’s printer requires some
precautions:
1. Use only the printer paper provided by electrolyte analyzer

distributor.
2. Do not permit the printer to operate without printer paper
installed.
3. In this situation go to SETUP MENU, press default setting enter
the PRINTER section and select the required option “Y” or “N” for
Printer ON /OFF. The absence of paper during printer operation
will cause premature printer failure.
17
4. Do not apply any lubricants, grease, or other materials to the

printer assembly under any circumstances.
Failure to comply with the above requirements will void the printer
warranty.
Printer setup
Figure 10
E. Power Up:
Confirm that the ST-200 PLUS (5 Ch) electrolyte analyzer voltage setting
on the rear cover matches the outlet voltage. When all the components
have been assembled, plug the ST-200 PLUS (5 Ch) electrolyte analyzer
into a grounded outlet. When the ST-200 PLUS (5 Ch) electrolyte analyzer
is plugged in, the power is on. The Na/K/iCa/Cl/Li/pH analyzer will
display,
SENSA CORE
ELECTROLYTE ANALYZER
Ver : XXXXX
When the ST-200 PLUS (5 Ch) electrolyte analyzer displays SYSTEM

UNCALIBRATED PLEASE CALIBRATE, installation is complete.
After initial setup, do not unplug the ST-200 PLUS (5 Ch) electrolyte
analyzer unless it must be moved to another location.
18
Diagram of Back view connections:
Figure 11
19
OPERATING THE ANALYZER
3. Operating the Analyzer
A. calibration
The instrument has to be calibrated when it is switched ON or it

automatically calibrates on set calibrated frequency after four hours or
eight hours as per set frequency.
Following is the calibration procedure:

A. Calibration
Switch ON the instrument
INITIALIZING
↓
SELF CHECK
↓
ACQUIRING RANGES
Please wait…
SENSACORE
System Uncalibrated
Please Calibrate….
Na* K* iCa* Cl* pH*

Press CALIBRATE key
↓
CALIBRATE
Y→ Continue N→ Exit
Press Y
↓
Searching pack
↓
PUMPING AIR
↓
PUMPING CAL A…
↓
20
PUMP CAL A TEST

Pump CAL = 8811
↓
PUMPING AIR
To clean the electrodes
↓
PUMPING CAL A
↓
CAL B MEASUREMENT
… 3 cycles
Air, Liquid
Air, Liquid
Air, Liquid
Na = ______ mV
K = ______ mV
iCa= ______ mV
pH = ______ mV
Cl = ______ mV
Displays CAL B milli volts on display

E.g.: Na= 36.751 mV
K = 41.087 mV
iCa = 67.705 mV
pH = -4.285 mV
Cl= 79.703 mV
↓
CAL A MEASUREMENT
↓
…3 cycles
Air, Liquid
Air, Liquid
Air, Liquid
Na = ______ mV
K = ______ mV
iCa= ______ mV
pH = ______ mV
Cl = ______ mV
Displays CAL A milli Volts on display

E.g: Na = 56.009 mV
K = 58.227 mV
iCa = 57.662 mV
pH = -34.634 mV
Cl = 62.058 mV
↓
21
CALIBRATION SLOPE
Na = _______ mV/decade
K = _______ mV/decade
iCa = _______ mV/decade
pH = _______ mV/decade
Cl = _______ mV/decade
Finally the instrument displays calibration slope and is ready for analysis.
E.g.: Calibration Slope
Na = 60.766 mV/decade
K = 54.981 mV/decade
iCa = 31.880 mV/decade
pH = 58.834 mV/decade
Cl = 54.182 mV/decade
If any electrode Is not calibrated then it will show **** beside that parameter in home
screen. So that we can clearly see that status of instruments.
B. Analyze Sample
To obtain patient’s sample, follow the procedure and precautions described

under sample handling and collection. Quality control material should be
run prior to patient sample analysis.
Aspirating sample
Figure 12
22
After calibration, press Analyze key
ANALYZE SAMPLE
Press Y
↓
Probe in Sample?
Press Y
↓
Remove Sample?
Y→ Continue
Press Y
↓
Positioning Sample
SAMPLE MEASUREMENT
Sample is positioned into the sensors

↓
Na = ______ mV
K = ______ mV
iCa= ______ mV
pH = ______ mV
Cl = ______ mV
Displays given SAMPLE milli Volts on display.
E.g: Na = 54.900 mV
K = 58.832 mV
iCa = 56.009 mV
pH = -42.397 mV
Cl= 65.587 mV
Processing…
23
Na = ______ mV
K = ______ mV
iCa= ______ mV
pH = ______ mV
Cl = ______ mV
Displays CAL A milli Volts on display.
Eg. Na = 56.009 mV
K = 57.723 mV
iCa =57.017 mV
pH = -34.936 mV
Cl = 61.857 mV
↓
ANALYZE SAMPLE
Na = 140.912 mmol/L
K = 4.324 mmol/L
iCa = 1.203 mmol/L
pH = 7.362
Cl = 101.800 mmol/L
Result Outside the measurement ranges as follows will not be reported

WHOLE HAPERIN BLOOD
Na+ : 20.0 - 250.0 mmol/L

K+ : 0.2 - 40.0 mmol/L
iCa++ : 0 - 10.0 mmol/L
Cl- : 20.0 - 200.0 mmol/L
Li+ : 0 - 5.0 mmol/L
pH : 5.0 - 9.0
SERUM, PLASMA & CSF
Na+ : 20.0 - 250.0 mmol/L

K+ : 0.2 - 40.0 mmol/L
iCa++ : 0 - 10.0 mmol/L
Cl- : 20.0 - 200.0 mmol/L
Li+ : 0 - 5.0 mmol/L
pH : 5.0 - 9.0
If iCa unit is in mg/dL, then the measurement range is 0-40 mg/dL.
24
C. Daily Cleaner
The fluid path must be cleaned after every 25 samples to remove protein
deposition from the sensors. The daily cleaner is required and is essential
for trouble-free analyzer operation. Following is the procedure of daily
cleaner.
Giving daily cleaner
Figure 13
MENU
------
1.DIAGNOSTICS
2.USER MENU
3.SETUP MENU
4.PARAMETERS
5.SELECT iCa UNIT
Press 1
↓
DIAGNOSTICS
-----------
1.DAILY CLEANER
2.MAINTENANCE
3.SENSORS
4.FLOW
5.HARDWARE
6.REAGENT PACK
Press 1
25
DAILY CLEANER
PROBE MOVING TO
ASPIRATION POSITION
Probe in Cleaner?
Aspirate cleaning sol. & press Y
Aspirating
Please Wait . . .
↓
Remove Cleaner?
Y→ Continue
↓
Press Y
CLEANING
Performing…….
↓
PUMPING AIR
↓
PUMPING CAL A
↓
PUMPING AIR
↓
PUMPING CAL A
↓
Clean Cycle Performed

↓
PUMPING AIR
↓
PUMPING CAL A…
Pump calibration done & display pump steps.
Clean electrodes
↓
26
CAL B MEASUREMENT
…….3 cycles of Cal B
Air, Liquid
Air, Liquid
Air, Liquid
↓
Na = ______ mV
K = ______ mV
iCa= ______ mV
pH = ______ mV
Cl = ______ mV
Displays CAL B milli Volts

↓
CAL A MEASUREMENT
….......3 cycles of Cal A
Air, Liquid
Air, Liquid
Air, Liquid
↓
Na = ______ mV
K = ______ mV
iCa= ______ mV
pH = ______ mV
Cl = _______ mV
Displays CAL A milli Volts

↓
SEARCHING PACK
↓
PUMPING AIR
↓
PUMPING CAL A…
↓
PUMP CAL A TEST
Pump CAL = 8658
↓
PUMPING AIR
↓
PUMPING CAL A
↓
CAL B MEASUREMENT
↓
… 3 cycles
Air, Liquid
Air, Liquid
Air, Liquid
27
Na = ______ mV
K = ______ mV
iCa= ______ mV
pH = ______ mV
Cl = _______ mV
CAL A MEASUREMENT
↓
…3 cycles
Air, Liquid
Air, Liquid
Air, Liquid
Na = ______ mV
K = ______ mV
iCa= ______ mV
pH = ______ mV
Cl = ______ mV
CALIBRATION SLOPE
pH = _______ mV/decade
N→ Exit
Finally displays calibration slope & instrument is ready for analysis.
28
D. Standby
Placing the ST-200 PLUS (5 Ch) in STANDBY mode protect reagents by

discontinuing automatic calibrations. The analyzer does, however, draw a
small amount of calibrant A solution & calibrant B solution to prevent the
electrodes from drying out. To place the ST-200 PLUS (5 Ch) in standby
mode, press STANDBY key.
After pressing STANDBY key

↓
*** STANDBY ***
N→ Exit
(Analyzer enters in a standby mode, if press N, analyzer exits from standby

mode and ask for calibration)
EXITING
STANDBY MODE
Pressing Standby Button:
Figure 14
29
E. Sub Menus
i. Diagnostics
30
ii. User Menu
31
iii. Setup Menu
32
iv. Parameters
Note: Star (*) Indicates Blank
33
F. Analyze Urine
To obtain urine sample, follow the procedure and precautions described

under sample handling and collection.
Dilute 200 microlitres of urine specimen with 1 mL (1000 microlitres) of

urine diluent’s (this corresponds to 1 part urine and 5 parts urine
diluent’s resulting in 1:5 dilutions).
Note: In urine mode only Na, K, Cl parameters are measured but not all.
After dilution change Analyze type to URINE mode by following below steps
Press MENU
↓
MENU
------
1.DIAGNOSTICS
2.USER MENU
3.SETUP MENU
4.PARAMETERS
5.SELECT iCa UNIT
↓
SETUP MENU
----------
1.ANALYZE TYPE
2.DATE/TIME
3.PRINTER
4.PRINT-PARAMETER
5.PRINT-mV
6.PATIENT-ID
↓
ANALYZE TYPE
-----------
1.SAMPLE-TUBE
2.URINE
3.SAMPLE-SYRINGE
4.QUALITY CONTROL
5.NON DETECTABLE
↓
ANALYZE TYPE : URINE
N→ Exit
34
Aspirating urine:
Figure 15
Set instrument in Urine type as follows:
ANALYZE URINE
↓
Press Y
Probe in Urine?
Press Y
↓
Please Wait ...
↓
Remove Urine?
Y→ Continue
35
Positioning Urine
↓
URINE MEASUREMENT
Na = ________ mV
K =________ mV
Cl =________ mV
N→ Exit
milli Volts of urine sample will be display.
e.g. Na: 31.609 mV

K: 81.114 mV
Cl: 77.787 mV
↓
Processing…
Na = ______ mV
K = _____ mV
Cl = _____ mV
N→ Exit
milli Volts of Cal A will be displayed on the display.
e.g. Na: 55.706 mV

K: 57.017 mV
Cl: 61.857 mV
↓
ANALYZE URINE
Na= 350.187 mmol/L

K= 72.758 mmol/L
Cl= 347.085 mmol/L
Result Outside the measurement ranges as follows will not be

reported
URINE RANGE
Na+ 35 - 1000 mmol/L
K+ 50 - 200 mmol/L
Cl- 25 - 500 mmol/L
36
G. Stored Results
Press MENU 2. USER MENU 4. PRINT STORED RESULT
 Sample Results
 QC Results
 Urine Results
The ST-200 PLUS (5 CH) has data storage and recovery capability of 1
Lakh patients results, 25000 Urine results and 100 QC results. All results
from the oldest day will be deleted when the storage capacity has been
reached. Therefore it is important to request a printout of data as the
storage capacity is approached if you desire to retain a copy of all the
stored results.
SEARCHING PATIENT ID:
ST -200 Plus is having a feature to search patient results by entering

Patient ID. User should enter patient ID (up to 5 numeric digits),
after Pressing Y it will display results on screen, user can take print out by
pressing Y button again.
STORED SAMPLE RESULTS

Please Enter ‘5’ digit
Patient ID :
XXXXX
Y→ Continue N→ Clear
Searching ...
↓
P.ID: XXXXX
Na = ________
K = ________
iCa = ________
Cl = _________
Li = ________
pH = ________
Y→ Print N→ Exit
37
Press ‘ Y’ to print displayed result or press ‘N’ to exit.

H. Reagent Pack Consumption
The ST-200 PLUS (5 Ch) monitors fluid consumption automatically. The

percentage of the reagent pack that has been consumed can be checked
whenever required by answering ‘Y’ to REAGENT PACK CONSUMPTION,
the percentage of the reagent pack that has been consumed is displayed
and printed. The ST-200 PLUS (5 Ch) will indicate REAGENT PACK LOW,
when 80% of the reagent pack solution has been consumed. When 20% of
the reagent solution remains in reagent pack, the ST-200 PLUS (5 Ch) will
instruct the operator to change pack by displaying message as REPLACE
NEW REAGENT PACK (the message will display but not print). The ST-200
PLUS (5 Ch) will not allow further analysis until a new solution pack has
been installed.
Reagent Pack Installation
Do not squeeze the reagent pack.
Hold the reagent pack in your left hand and place reagent pack into the
track on the base of the analyzer to guide the positioning of the reagent
pack; slide the reagent pack firmly to right plugging it into the valve
module. Push the reagent pack until it stops.
Do not use expired reagent pack. The electrode should be exposed to

ST-200 PLUS (5 Ch) reagent all time so that it will protect electrode from
getting damage.
Incompatible pack
If unauthorized reagent pack is trying to use in analyzer then it will

display Incompatible pack on screen. Use authorized reagent pack.
I. Quality control
Use ST-200 PLUS (5 Ch) Quality controls on a daily basis to verify the
accuracy and precision of your analyzer. If the ST-200 PLUS (5 Ch) is
calibrated and your quality control results are within specified range, you
can be confident in the results obtained.
Run all the control levels daily. Data may be stored in memory for future
statistical analysis.
Refer to the Quality control ranges in performance certificate provided with

the quality control Solutions.
Do not use control materials containing ethylene glycol and other stabilizer,
as they may give incorrect results and/or damage the
ST-200 PLUS (5 Ch) electrodes.
38
To perform quality control sample set up QC SAMPLE TYPE in set up

menu.
J. Operator Functions
Following are the steps to operate ST-200 PLUS (5 Ch) in the form of flow
diagram.
a) Diagnostics
1. DAILY CLEANER :
The diagnostics routine permits the operator to check and test key
operating functions of the ST-200 PLUS (5 Ch).
Following is the flow chart of diagnostics menu.
MENU
------
1.DIAGNOSTICS
2.USER MENU
3.SETUP MENU
4.PARAMETERS
5.SELECT iCa UNIT
Press 1
↓
DIAGNOSTICS
-----------
1.DAILY CLEANER
2.MAINTENANCE
3.SENSORS
4.FLOW
5.HARDWARE
6.REAGENT PACK
Press 1
↓
DAILY CLEANER
↓
PROBE MOVING TO
ASPIRATION POSITION
↓
Probe in Cleaner?
Aspirate cleaning sol. & press Y

↓
Aspirating
Please Wait…
↓
Remove Cleaner?
Y→ Continue
↓
39
Press Y
CLEANING
Performing…….
↓
PUMPING AIR
↓
PUMPING CAL A
↓
PUMPING AIR
↓
PUMPING CAL A
↓
Clean Cycle Performed

↓
PUMPING AIR
↓
PUMPING CAL A…
Pump calibration done & display pump steps.
Clean electrodes
↓
CAL B MEASUREMENT
…….3 cycles of Cal B
Air, Liquid
Air, Liquid
Air, Liquid
↓
Na = ______ mV
K = ______ mV
iCa= ______ mV
pH = ______ mV
Cl = ______ mV
Displays CAL B milli Volts

↓
CAL A MEASUREMENT
….......3 cycles of Cal A
Air, Liquid
Air, Liquid
Air, Liquid
↓
Na = ______ mV
K = ______ mV
iCa= ______ mV
pH = ______ mV
Cl = ______ mV
Displays CAL A milli Volts

↓
SEARCHING PACK
↓
40
PUMPING AIR
↓
PUMPING CAL A…
↓
PUMP CAL A TEST

Pump CAL = 8811
↓
PUMPING AIR
↓
PUMPING CAL A
↓
CAL B MEASUREMENT
… 3 cycles
Air, Liquid
Air, Liquid
Air, Liquid
↓
Na = ______ mV
K = ______ mV
iCa= ______ mV
pH = ______ mV
Cl = _______ mV

↓
CAL A MEASUREMENT
… 3 cycles
Air, Liquid
Air, Liquid
Air, Liquid
↓
Na = ______ mV
K = ______ mV
iCa= ______ mV
pH = ______ mV
Cl = ______ mV

↓
CALIBRATION SLOPE
pH = ______ mV/decade
N→ Exit
41
Finally displays calibration slope & instrument is ready for analysis.

2. MAINTENANCE:
Whenever operator wants to switch off the instrument then he should

activate maintenance mode then he can switch off instrument.
MENU
------
1.DIAGNOSTICS
2.USER MENU
3.SETUP MENU
4.PARAMETERS
5.SELECT iCa UNIT
Press 1
↓
DIAGNOSTICS
-----------
1.DAILY CLEANER
2.MAINTENANCE
3.SENSORS
4.FLOW
5.HARDWARE
6.REAGENT PACK
Press 2
↓
ENTERING MAINTENANCE
Please wait …
Cleared liquid from the tubing

↓
***MAINTENANCE ***
N→ Exit
Press N it will display
↓
EXITING MAINTENANCE
Please wait . . .
↓
SENSACORE
System uncalibrated
Please calibrate ….
Na K iCa Cl pH
Press Calibrate Key.
42
3. SENSORS :
Sensors is used to check mV of the electrodes for each reagent (CAL A

/CAL B) separately
MENU
------
1.DIAGNOSTICS
2.USER MENU
3.SETUP MENU
4.PARAMETERS
5.SELECT iCa UNIT
Press 1
↓
DIAGNOSTICS
------------
1.DAILY CLEANER
2.MAINTENANCE
3.SENSORS
4.FLOW
5.HARDWARE
6.REAGENT PACK
Press 3
↓
SENSORS
---------
1.SENSOR CAL A-mV
2.SENSOR CAL B-mV
N→ Exit
press1
↓
SENSOR CAL A-mV
↓
CAL A MESUREMENT
… 3 cycles of Cal
A
Air, Liquid
Air, Liquid
Air, Liquid
↓
Na = _______ mV
K = _______ mV
iCa = _______ mV
pH = ______ mV
Cl = _______ mV
N→ Exit
Displays milli Volts of cal A
Press N
↓
SENSORS
---------
1.SENSOR CAL A-mV
43
2.SENSOR CAL B-mV
Press 2 ↓
SENSOR CAL B-mV
CAL B MEASUREMENT
…. 3 cycles of Cal B
Air, Liquid
Air, Liquid
↓ Air, Liquid
Na = _______ mV
K = _______ mV
iCa = _______ mV
pH = ______ mV
Cl = ______ mV
N→ Exit
Displays milli Volts of cal B
4. FLOW:
It is used when, inserting new reagent pack or troubleshooting flow issues in instrument
by priming cal A, cal B & ALL.
MENU
------
1.DIAGNOSTICS
2.USER MENU
3.SETUP MENU
4.PARAMETERS
5.SELECT iCa UNIT
Press 1
↓
DIAGNOSTICS
-----------
1.DAILY CLEANER
2.MAINTENANCE
3.SENSORS
4.FLOW
5.HARDWARE
6.REAGENT PACK
Press 4
↓
Reagent Priming
FLOW
------
1.REAGENT PRIMING
2.PUMP CALIBRATION
Press 1
↓
44
REAGENT PRIMING
--------------
1.PRIME CAL A
2.PRIME CAL B
3.PRIME ALL
N→ Exit
↓
Press 1
PRIMING CAL A
Please wait …
Air is aspirated followed by Cal A through electrode housing.
PRIMING CAL A
Priming Completed
N→ Exit
Press N
↓
REAGENT PRIMING
--------------
1.PRIME CAL A
2.PRIME CAL B
3.PRIME ALL
N→ Exit
↓
Press 2
PRIMING CAL B
Please wait …
↓
Air is aspirated followed by CAL B through electrode housing.
PRIMING CAL B
Priming Completed
N→ Exit
↓
Press N
PRIMING CAL B
Priming …
↓
REAGENT PRIMING
--------------
1.PRIME CAL A
2.PRIME CAL B
3.PRIME ALL
N→ Exit
45
Press 3
↓
PRIMING CAL B
Please wait …
↓
Air is aspirated followed by CAL B through electrode housing.
PRIMING CAL A
Please wait….
↓
Air is aspirated followed by CAL A through electrode housing.
PRIMING CAL A
Priming Completed
N→ Exit
Press N
↓
REAGENT PRIMING
--------------
1.PRIME CAL A
2.PRIME CAL B
3.PRIME ALL
N→ Exit
↓
Pump Calibration
FLOW
------
1.REAGENT PRIMING
2.PUMP CALIBRATION
Press 2
↓
PUMPING AIR
PUMPING CAL A
PUMP CAL A TEST
Pump CAL = 8836
N→ Exit
Instrument performs pump calibration using Cal A & displays the pump steps. If Pump
CAL is greater than 13000 then displays PUMP CAL ERROR, change the pump tube.
46
5. HARDWARE:
MENU
------
1.DIAGNOSTICS
2.USER MENU
3.SETUP MENU
4.PARAMETERS
5.SELECT iCa UNIT
Press 1
↓
DIAGNOSTICS
------------
1.DAILY CLEANER
2.MAINTENANCE
3.SENSORS
4.FLOW
5.HARDWARE
6.REAGENT PACK
Press 5
↓
Sample Detector
HARDWARE
------------------
1.SAMPLE DETECTOR
2.PUMP MODULE
3.SAMPLER
4.SOFTWARE VERSION
Press 1
↓
SAMPLE DETECTOR TEST
PUMPING AIR
↓
SAMPLE DETECTOR TEST
Air Detected
↓
PUMPING LIQUID
Liquid flows through bubble detector
↓
Liquid Detected
N→ Exit
47
Pump Module
HARDWARE
------------------
1.SAMPLE DETECTOR
2.PUMP MODULE
3.SAMPLER
4. SOFTWARE VERSION
Press 2
↓
PUMP SPEED → FAST
PUMP SPEED → SLOW
N→ Exit
Pump rotates and checks its status.
Sampler
HARDWARE
------------------
1.SAMPLE DETECTOR
2.PUMP MODULE
3.SAMPLER
4.SOFTWARE VERSION
Press 3
↓
PROBE MOVING TO
ASPIRATION POSITION
PROBE MOVING TO
REST POSITION
N→ Exit
Probe comes down and moves back to the rest position. Thus check the status of
unislider.
Software version:
HARDWARE
------------------
1.SAMPLE DETECTOR
2.PUMP MODULE
3.SAMPLER
4.SOFTWARE VERSION
Press 4
48
↓
SOFTWARE VERSION:
XXXXX
Date : DD-MM-YY
N→ Exit
It shows the version & date.
6. REAGENT PACK:
It shows the information of reagent pack like consumption, reagent pack lot no or
pack volume & information about CAL A, CAL B concentration.
MENU
------
1.DIAGNOSTICS
2.USER MENU
3.SETUP MENU
4.PARAMETERS
5.SELECT iCa UNIT
Press 1
↓
DIAGNOSTICS
------------
1.DAILY CLEANER
2.MAINTENANCE
3.SENSORS
4.FLOW
5.HARDWARE
6.REAGENT PACK
Press 6
↓
REAGENT PACK
------------
1.REAGENT PACK ID
2.REAGENT PACK INFO
3.REAGENT PACK
CONSUMPTION
Press 1
↓
Please Wait ...

↓
REAGENT PACK ID
---------------
:905F6C03000007E0
OEM/NON OEM: NON OEM
DISTRIBUTOR ID: XXI
LOT NO:XXXXX
PACK VOLUME: 800 mL
N→ Exit
Press N
49
REAGENT PACK
-------------
1.REAGENT PACK ID
2.REAGENT PACK INFO
3.REAGENT PACK
CONSUMPTION
Press 2
↓
Please Wait ...
↓
REAGENT PACK INFO
----------------
MANUFACTURED ON:
******
EXPIRY DATE:
******
Y→ Continue
Reagent pack manufacturing and expiry date is displayed.

Press Y
↓
CAL A CONCENTRATION
------------------
Na : 147.0
K : 4.10
iCa : 1.40
Cl : 127.00
Li : 0.30
pH : 7.400
Y→ Continue
Concentration of Cal A is displayed.

Press Y
↓
CAL B CONCENTRATION
------------------
Na : 70.00
K : 2.00
iCa : 2.90
Cl : 60.00
Li : 2.00
pH : 6.78
N→ Exit
Concentration of Cal B is displayed.

Press N
REAGENT PACK
------------
1.REAGENT PACK ID
2.REAGENT PACK INFO
3.REAGENT PACK
CONSUMPTION
Press 3
50
↓
Please Wait ...
REAGENT PACK CONSUMPTION

-----------------------
Solution left : 98%
Consumption of reagent is displayed in %.

Press N
↓
NO OF SAMPLES : 10
TOTAL SAMPLES : 821
N→ Exit
Total number of performed samples is displayed.
51
B) USER MENU
Users menu permits the operator to check and test key operating functions of the
ST-200 PLUS (5 Ch).
Following is the flow chart of users menu.
MENU
------
1.DIAGNOSTICS
2.USER MENU
3.SETUP MENU
4.PARAMETERS
5.SELECT iCa UNIT
Press 2
↓
USER MENU
----------
1.NORMAL RANGES
2.CALIBRATION FREQUENCY
3.SIPPING FREQUENCY
4.PRINT STOREDRESULTS
5.AUTO-STANDBY
6.CORRECTION FACTOR
Press 1
↓
NORMAL RANGES
-------------------------
1.RESET RANGES
2.USER SETTINGS
Please make a note that if you press the RESET RANGES then instrument reset all the
ranges to Default factory settings.
Press 1
↓
RESETTING RANGES
Please wait…..
↓
RANGES RESETTED
↓
AQUIRING RANGES
Please wait….
NORMAL RANGES: USER SETTINGS
MENU
------
1.DIAGNOSTICS
2.USER MENU
3.SETUP MENU
4.PARAMETERS
5.SELECT iCa UNIT
Press 2
↓
52
USER MENU
----------
1.NORMAL RANGES
3.SIPPING FREQUENCY
4.PRINT STORED RESULTS
5.AUTO-STANDBY
6.CORRECTION FACTOR
Press 1
↓
NORMAL RANGES
-------------------------
1.RESET RANGES
2.USER SETTINGS
Press 2
↓
USER SETTINGS
--------------
1.SAMPLE RANGES
2.URINE RANGES
3.QC1 RANGES
4.QC2 RANGES
5.QC3 RANGES
Press 1
↓
SAMPLE RANGES
1.Na
2.K
3.iCa
4.Cl
5.Li
6.pH
N → Exit
Press required parameter to be changed and enter its maximum and minimum ranges.
Example:
Press 1
↓
SAMPLE RANGES
Na MAX: 150.00
Na MIN : 135.00
Y→ Edit N→ Exit
Press Y
↓
-------EDIT-------
Na Max : 150.00
Na Min : 135.00
1→Mx 2→Mn N→Exit
Press 1
↓
Na MAX:
Y→Save N→Clear
Enter the value and press ‘ Y ‘ to Save and ‘ N ‘ to Clear.

Similarly, for all the parameters in Sample ,Urine & QC Ranges.
53
CALIBRATION FREQUENCY
Instrument will perform automatic calibration for every 4 hours /8 hours for good
performance of the instrument.
USER MENU
----------
1.NORMAL RANGES
3.SIPPING FREQUENCY
5.AUTO-STANDBY
6.CORRECTION FACTOR
Press 2
↓
CALIBRATION FREQUENCY
STATUS : 4 HOURS
Y→ 4 HOURS N→ 8 HOURS
Press Y to select 4 hours or N to select 8 hours calibration frequency.
SIPPING FREQUENCY
For every 30min /1 hour instrument takes 200ul of liquid to make electrodes wet
this is called sipping. Here we can select the sipping time frequency
USER MENU
----------
1.NORMAL RANGES
3.SIPPING FREQUENCY
5.AUTO STANDBY
6.CORRECTION FACTOR
Press 3
↓
SIPPING FREQUENCY
STATUS : 30 MINS
Y→ 30 MINS N→ 1 HOUR
54
PRINT RESULTS
USER MENU
----------
1.NORMAL RANGES
3.SIPPING FREQUENCY
5.AUTO-STANDBY
6.CORRECTION FACTOR
Press 4
PRINT STORED SAMPLE RESULTS:
PRINT STORED RESULTS

-------------------
1.Sample Results
2.QC Results
3.Urine Results
Press 1
↓

Please Enter ‘5’ Dig
Patient ID:
XXXXX
Y→ Continue N→
Clear
Enter Patient Id maximum up to 6 numeric digits which you want to search
Press Y
↓
Searching ...
↓
P.ID :XXXXX
Na= 135.89
K= 4.215
iCa= 1.083
Cl = 92.559
Li= 0.00
pH = 7.06
Press ‘ Y ‘ to print displayed result or press ‘ N ‘ to shift to next result screen.
55
PRINT STORED QC RESULTS:
For storing QC results separately user must select ANALYZE TYPE to QULATIY
CONTROL in settings menu.

-------------------
1.Sample Result
2.QC Result
3.Urine Results
Press 2
↓
-- QC Samples --
1. Last 1-25 samples

N→ Next
Depending upon the result required select the range option.

Press 1
↓
RESULT : 00
Y→ Next N→ Exit
Press Y
↓
RESULT : 01
Na= 135.89
K= 4.215
iCa= 1.083
Cl = 92.559
Li= 0.00
pH = 7.06
↓
RESULT : 01
Na= 117.48
K= 1.848
iCa= 1.918
Cl = 74.29
Li= 0.00
pH = 7.06
Y→ Next N→ Exit
Press ‘ Y ‘ to display next result or press ‘ N ‘ to Exit.

Press ‘ Y ‘
↓
56
RESULT : 02
Na= 135.89
K= 4.215
iCa= 1.083
Cl = 92.559
Li= 0.00
pH = 7.06
Y→ Print N→ Next
Press ‘ Y ‘ to print displayed result or press ‘ N ‘ to obtain next result.
PRINT STORED URINE RESULTS:

-------------------
1.Sample Results
2.QC Results
3.Urine Results
Press3
↓
STORED URINE RESULTS
Please enter ‘5’ digit
Patient ID:
XXXXX
Y→ Continue N→
Clear
Enter Patient Id maximum up to 5 numeric digits which you want to search
Press Y
↓
PATIENT ID :XXXXX
Na= 352.88
K= 64.52
Cl= 349.94
Y→ Print N→
Exit

Press N.
AUTO STANDBY
AUTO STANDBY option is used to ON the STANDBY mode automatically,

which depends on calibration frequency. If calibration frequency is 4 hrs, calibration
occurs after 4 hrs from the previous calibration after that analyzer automatically goes into
STANDBY mode.
MENU
------
1.DIAGNOSTICS
2.USER MENU
3.SETUP MENU
57
4.PARAMETERS
5.SELECT iCa UNIT
Press 2
↓
USER MENU
----------
1.NORMAL RANGES
3.SIPPING FREQUENCY
5.AUTO-STANDBY
6.CORRECTION FACTOR
Press 5 for Auto Standby
↓
AUTO-STANDBY
STATUS: ON
Y→ON
N→OFF
Press Y to ON and N to OFF
CORRECTION FACTOR
USER MENU
----------
1.NORMAL RANGES
3.SIPPING FREQUENCY
5.AUTO-STANDBY
6.CORRECTION FACTOR
Press 6
↓
SLOPE/INTERCEPT
1. Na
2. K
3. Cl
4. iCa
5. Li
6. pH
Press 1
↓
SLOPE/INTERCEPT
---------------
SLOPE FACTOR:
[ 01.00 ]
INTERCEPT:
+[ 00.00 ]
Y→ SET N→ Next
58
Note: - By default Slope factor value is 01.00 & Intercept is 00.00.
SLOPE FACTOR:
Slope factor is used for multiplying the factor with the result obtained.
Figure 16
In the above graph, green line indicates the normal/reference state (All the control
levels lie with its range as per the Q.C sheet provided in the Q.C box) and red line
indicates the error/out-off state (Electrodes are reading either higher or lower).
After running all the three controls only, we can confirm that whether slope
factor/intercept should be added depending upon its readings.
In the above graph level-1, level-2 and level-3 of any parameter is reading on
higher side. In this case we have to add slope factor between 0.5 to 1. So, that level-1,
level 2 and level 3 results will be multiplied by that factor.
E.g.: In the above graph, when all the three Q.C levels are analyzed. We have got Na on
higher side. In this case, we have to insert slope factor of 0.92. Now, 0.92 will be
multiplied with the result of Na (e.g.: 130*0.92=119).
After adding slope factor we should run all the three controls and verify whether
selected parameter (parameter which needed correction) lie within its range. Slope factor
should be added and verified until the parameter lie in its range.
If level-1, level-2 and level-3 of Na is reading on lower side (i.e. reverse of above
case). Then, we have to add slope factor between 1to 1.5. So, that level-1, level 2 and level
3 results will be multiplied by that factor.
Y=mX+C
59
Where, m = slope. If we add slope factor from 0.5 to 1, then our output(Y) will be
decreased. Since, slope factor will be multiplied with X (obtained result). Similarly, if we
add slope factor from 1 to 1.5, then our output will be increased. Since, slope factor will
be multiplied with X (obtained result).
Note: Slope factor ranges from 0.5 to 1.5 for Na, K, iCa, Li, Cl parameters individually.
INTERCEPT:
Intercept is used for adding the factor with the result obtained.
Figure 17
(i.e.) y = mx + c
Where m is the slope of the line and C is the y-intercept, which is the y-coordinate of
the location where line crosses the y axis.
For increasing/decreasing all the control values to desired level we have to select
+ or -10 variation for Na, Cl & + or – 0.5 variation in K, iCa, Li. In the above graph of
intercept, Na is reading all the three control levels higher approximately by 10. In this
case we have to subtract intercept as 10.00 and verify it by running all three controls.
Similarly, for all the parameters intercept can be added according to their requirement
and respectively intercept ranges.
Note: - By default Slope factor value is 01.00 & Intercept is 00.00
SLOPE/INTERCEPT
---------------
SLOPE FACTOR:
[ 01.00 ]
INTERCEPT:
+[ 00.00 ]
Y→ ‘+’ N→ ‘- ‘
60
Select + or – 10 variation for Na, Cl & + or – 0.5 variation in K, pH, iCa, Li according to
the necessity for result.
Press N
C) SETUP MENU
Setup menu permits the operator to check and test key operating functions of the ST-200
PLUS (5 Ch).
Following is the flow chart of setup menu.
MENU
------
1.DIAGNOSTICS
2.USER MENU
3.SETUP MENU
4.PARAMETERS
5.SELECT iCa UNIT
Press 3
↓
SETUP MENU
----------
1.ANALYZE TYPE
2.DATE/TIME
3.PRINTER
4.PRINT-PARAMETER
5.PRINT-mV
6.PATIENT-ID
Press 1
↓
ANALYZE TYPE
------------
1.SAMPLE - TUBE
2.URINE
3.SAMPLE – SYRINGE
4.QUALITY CONTROL
5.NON DETECTABLE
Press 1
↓
ANALYZE TYPE
------------
SAMPLE -TUBE
N→ Exit
Press N
↓
ANALYZE TYPE
-----------
1.SAMPLE - TUBE
2.URINE
3.SAMPLE - SYRINGE
4.QUALITY CONTROL
5.NON DETECTABLE
Press 2
↓
61
ANALYZE TYPE : URINE

N→ Exit
Press N
↓
ANALYZE TYPE
------------
1.SAMPLE - TUBE
2.URINE
3.SAMPLE –
SYRINGE
4.QUALITY CONTROL
5.NON DETECTABLE
Press 3
↓
ANALYZE TYPE
-------------
SAMPLE –SYRINGE
N→ Exit
Press N
↓
ANALYZE TYPE
------------
1.SAMPLE - TUBE
2.URINE
4.QUALITY CONTROL
5.NON DETECTABLE
Press 4
↓
ANALYZE TYPE
-------------
QUALITY CONTROL
N→ Exit
Press N
↓
ANALYZE TYPE
------------
1.SAMPLE - TUBE
2.URINE
4.QUALITY CONTROL
5.NON DETECTABLE
Press 5
Non Detectable
If there is any sample which consists of high Bilirubin (Lipemic samples)

sometimes bubble detector will not detect sample gives air in sample, and then change
the mode to NON DETECTABLE & run sample. Please make a note that after completion
of sample analyze setup analyze to SERUM mode.
62
ANALYZE TYPE
-------------
NON DETECTABLE
N→ Exit
Date / Time:
By using this option user can change the Date & Time of the instrument. By
using Y button we can select the field which we want to change & by using N button we
can change the field.
SETUP MENU
-----------
1.ANALYZE TYPE
2.DATE/TIME
3.PRINTER
4.PRINT -PARAMETER
5.PRINT-mV
6.PATIENT-ID
Press 2
↓
DATE/TIME
------------------
DATE : MM DD, YY
TIME : HH:MM:SS
Y→SET N→NEXT
Press Y
Select month
Apr
Y→ SET N→ NEXT
Press Y
Select date
04
Y→ SET
Press Y
Select Year
15
Y →SET
DATE/TIME
------------------
DATE : Apr 04, 2015
TIME : ---:---:---
Y→SET N→NEXT
63
Set the Time: Hours

Select Hour:
11
Y→ SET
DATE/TIME
------------------
DATE : Apr 04, 2015
TIME : 11:---:---
Y→SET N→NEXT
Set the Time: Minutes

Select Minute:
47
Y→ SET
DATE/TIME
------------------
DATE : Apr 04, 2015
TIME : 11:47:---
Y→SET N→NEXT
Set the Time: Seconds

Select Seconds:
30
Y→ SET
DATE/TIME
------------------
DATE : Apr 04, 2015
TIME : 11:47:30
Y→SET N→NEXT
Press N
The date and time has been set.
Printer:
User can switch OFF the printer if not required.
SETUP MENU
-----------
1.ANALYZE TYPE
2.DATE/TIME
64
3.PRINTER
4.PRINT-PARAMETER
5.PRINT-mV
6.PATIENT-ID
Press 3
↓
PRINTER
STATUS : ON
Y→ON N→OFF
Press Y to ON, press N to OFF the printer status
SETUP MENU
-----------
1.ANALYZE TYPE
2.DATE/TIME
3.PRINTER
4.PRINT-PARAMETER
5.PRINT-mV
6.PATIENT-ID
Press 4
↓
PRINT PARAMETER
----------------
1.Na
2.K
3.Cl
4.iCa
5.Li
Y→ Next N→ Exit
If your required parameter is present in this screen then select the parameter by pressing
appropriate number, if not then press Y button to see more parameters.
PRINT PARAMETER
----------------
6. pH
7. ALL
N→ Exit
Press corresponding number for parameters details which to be analyzed and printed
And press N to exit.
Print –mV:
If user don’t want to print mill volts (mV) of CAL A, CAL B & CAL C, but only
wants results then Print –mV can be deselected.
65
SETUP MENU
------------
1.ANALYZE TYPE
2.DATE/TIME
3.PRINTER
4.PRINT-PARAMETER
5.PRINT-mV
6.PATIENT-ID
Press 5
↓
PRINT – mV
STATUS : ON
Y→ON N→OFF
Press Y to ON, press N to OFF the PRINT – mV status
Patient ID:
Patient ID should be ON to get previous stored results. if patient ID option is deselected

then instrument will not ask to enter patient ID while analyzing.
SETUP MENU
-----------
1.ANALYZE TYPE
2.DATE/TIME
3.PRINTER
4.PRINT-PARAMETER
5.PRINT-mV
6.PATIENT-ID
Press 6
↓
PATIENT-ID
-----------
STATUS : OFF
------------
1-MANUAL 2-AUTO
N→ OFF
 We can set manual & auto status for PATIENT ID (i.e.) press 1 for AUTO status, it
takes the PATIENT ID spontaneously in serial wise for each sample without giving
PATIENT ID.
 In MANUAL status (i.e.) we have to provide PATIENT ID for each sample as per
requirement.
D) Parameters:
Depending upon requirement change model of the analyzer ensure that

Electrode staging should be done according to the order in Parameter
option.
Where * indicates blank electrode
Press Menu
66
MENU
------
1.DIAGNOSTICS
2.USER MENU
3.SETUP MENU
4.PARAMETERS
5.SELECT iCa UNIT
↓
Press 4
1. K Na * * *
2. K Na ** Cl
3. K Na iCa * *
4. K Na iCa * Cl
5. K Na iCa Li *
6. K Na iCa * pH
Y → Next N→ Exit
If your required model is present in this screen then select the model by
pressing appropriate number, if not then press Y button to see more
models
1. K Na iCa Li pH
2. K Na iCa pH Cl
N→ Exit
 By selecting the parameters as per requirement & arrange the

electrodes as per parameters.
Note: In 5th Model Li Electrode depends on Na and iCa Electrodes,

If Na or iCa or both Na & iCa not calibrated, Li won’t be analyzed.
E) Select iCa Unit:
Depending up on user requirement can select iCa measuring units to

mg/dL or mmol/L. By default it is in mmol/L
Press: MENU
MENU
------
1.DIAGNOSTICS
2.USER MENU
3.SETUP MENU
4.PARAMETERS
5.SELECT iCa UNIT
Press 5
67
↓
iCa UNITS
UNIT : mmol/L
Y → mg/dL N→ mmol/L
HANDLING AND COLLECTION
4. Sample Handling and Collection
Warning: Human body fluid specimens may be contaminated with HIV or

other pathogens. Treat all specimens and collection devices and tools as
biohazardous materials.
A. Whole Blood
Whole blood specimens should be drawn carefully to avoid hemolysis.

Elevated potassium values may indicate a hemolyzed specimen; if
hemolysis is suspected, a new sample should be drawn and analyzed.
Finger stick methods should be avoided since they can result in elevated
potassium values.
1. Collect the specimen by venipuncture into a Lithium-Heparin or

Sodium-Heparin (green-topped) evacuated blood collection tube. For
Lithium analysis, a sodium-Heparin (green-topped) tube must be
used. Do not use ammonium heparin, EDTA or NaF tubes. Note the
time of collection.
2. Mix the specimen by gently inverting and rotating the tube. Do not
shake.
3. Analyze specimens within one hour of collection; beyond this time

falsely elevated potassium levels may be obtained.
4. For analysis on Na/K/iCa/Cl/Li/pH analyzers, specimens should be

measured within 20 minutes of collection or store sample on ice for
up to 60 minutes.
B. Serum
1. Collect the specimen by venipuncture into an untreated (red-topped)

tube. Fill the tube to at least 2/3 of the total volume. Note the time
of collection.
68
2. Let blood stand for 20-30 minutes to allow clot formation.
3. Rim the clot with an applicator stick, then centrifuge the tube for
10-15 minutes and transfer the serum to a clean specimen tube.
4. Serum may be analyzed immediately or stored at 4ºC for 24 hours or

frozen at -20ºC for up to one week. Sample must be brought to room
temperature and mixed well before assaying.
To obtain accurate results, samples should be free of any clots, fibrin, etc.,
which would obstruct sample flow and affect results. The use of a serum
clearing agent is strongly recommended.
If a serum separator tube is utilized, care must be taken to avoid inserting

the sample probe into the gel layer. This can create obstructions in the
sample probe and electrodes.
Serum samples offer no advantage over whole blood specimens for stat
analysis but if the sample has to be stored, serum specimens are
preferable.
C. Plasma
1. Collect the specimen by venipuncture into a Sodium-Heparin (green-

topped) evacuated blood collection tube. The heparin level should
not exceed 15 IU per mL of tube volume. Note the time of collection.
2. Mix the specimen by gently inverting the tube. Do not shake.
3. Centrifuge the specimen within one hour of collection. Carefully

remove the top plasma layer for analysis. Use a Pasteur pipette or a
syringe fitted with a blunt-tipped needle for this procedure.
4. Analyze plasma samples within 4 hours of collection. Refrigerated

samples must be brought to room temperature and centrifuged prior
to analysis.
D. Urine
For complete sample preparation and storage information, the user should
refer to the standard clinical chemistry procedures published by NCCLS.
1. Follow standard clinical procedures for collection of random and 24-

hour urine specimens.
2. Refrigerate urine specimens until time of analysis.
3. Centrifuge urine specimens to remove cellular matter, crystals etc.
69
4. Dilute the urine specimen with one part of the supernatant to 6

parts of urine diluent. Urine must be diluted.
Do not attempt to analyze undiluted urine.
E. Expected Values
The values given in below tables (table 1 & 2) are intended to be used only
as a guide. Each laboratory or testing site should establish its own range
of normal values, taking into account factors such as age, sex, diet and
other determinants of electrolyte levels.
Whole Blood, Serum, Plasma
Sodium (Na+) 135 to 155 mmol/L

Potassium (K+) 3.5 to 4.5 mmol/L
Ionized Calcium (iCa++) 1.10 to 1.35 mmol/L
-
Chloride (Cl ) 94 to 110 mmol/L
Lithium (Li+) 0.3 to 1.30 mmol/L
pH pH 7.31 to 7.41
Table 1
Urine (mmoL/L)
Sodium (Na+) 40 to 220 mmol/L

Potassium (K+) 25 to 120 mmol/L
Chloride ( Cl-) 250 to 100 mmol/L
Table 2
70
MAINTENANCE
5. Maintenance
The ST-200 PLUS (5 Ch) electrolyte analyzer requires very little operator
maintenance. The only maintenance required is to run the daily cleaning
solution after every 25 sample of the day. All other maintenance is
performed by replacing the components as described in the schedule below.
When you wish to perform routine or any other maintenance function, go
to the MENU option and Select “Maintenance”. The instrument gets ready
for maintenance by priming the fluidic line with air. Follow the menu
prompts to perform the replacement.
71
PRINCIPLES OF OPERATION
6. Principles of Operation
A. ISE Theory
Electrolyte measurements in blood products were traditionally performed

using flame photometry, in which a sample, diluted with a known
concentration of a reference ion (usually lithium or cesium), is aerosolized
and passed through a flame which excites the cation. They re-emit the
energy as light of different frequencies; the amplitude of this emission is
proportional to the ion concentration in the sample. The development of
sodium and pH-selective glass, and selective organic compounds for
potassium and chloride, has permitted the development of sensors capable
of measuring biological fluids directly, throughout the physiological range.
These sensors are known as Ion selective electrodes.
The ST-200 PLUS (5 Ch) electrolyte analyzer measures sodium, potassium

and ionized calcium in biological fluids, using ion selective electrode
technology. The flow-through sodium electrodes contain plasma tube,
specially formulated to be sensitive to sodium ions. The flow-through
potassium electrodes employ a plastic tube, incorporating neutral carrier
ionophores. The flow-through Ionized Calcium electrode includes a plastic
tube, specially formulated to be selective to chloride ions. The potential of
each electrode is measured relative to a fixed, stable voltage established by
the silver/silver chloride reference electrode. An ion selective electrode
develops a voltage that varies with the concentration of the ion to which it
responds. The relationship between the voltage developed and the
concentration of the sensed ion is logarithmic, as expressed by the Nernst
equation:
E = Eº + RT Log (aC)
n
Where:
E = the potential of the electrode in sample solution
Eº = the potential developed under standard conditions
RT/nF = A temperature dependent “constant” termed the slope (s)
n = 1 for sodium, potassium, chloride, lithium and pH
72
n = 2 for calcium
Log = Base ten logarithm function
a = Activity coefficient of the measured ion in the solution
c = Concentration of the measured ion in the solution
A comparative method of measurement is utilized. First, the analyzer
measures the potential developed when the sample is aspirated through
the electrodes. Next, cal A is aspirated through the electrodes.
The difference in the two potentials is related logarithmically to the
concentration of ions in the sample divided by their respective
concentrations in the calibrant solution. Since the difference in potentials
and the concentration of the ions in the calibrant solution are known, the
analyzer can calculate the concentration of the ions in the sample solution,
in accordance with the Nernst equation, rewritten as:
E-Eº = S log (Ci (x) / Ci (s)) or Ci(x) = Ci(s) x 10ˆ (E-Eº)/ S
Where: E = ISE potential developed in sample solution

Eº = ISE potential developed in the calibrant solution
S = Electrode Slope calculated during calibration
Ci(x) = Concentration of ion “I” in the sample
Ci(s) = Concentration of ion “I” in the calibrant solution
“S”, the slope, is determined during calibration using Cal A and B, which
are known levels of ions.
The ST-200 PLUS (5 Ch) electrolyte analyzer aspirates and measures Cal A,
followed by Cal B. The ST-200 PLUS (5 Ch) electrolyte analyzer finally
aspirates additional Cal “A” solution and measures it. The two Cal “A”
readings are compared for drift and each measurement is checked for
instability (noise). The slope is calculated from the difference between the
second Cal A reading and the Cal B reading. A slope outside the built-in
software limits will be flagged and displayed as low or high. The ST-200
PLUS (5 Ch) electrolyte analyzer will detect excessive drifts or noise and
display the appropriate error message.
73
SPECIFICATIONS OF Na/K/iCa/Cl/Li/pH
7. Specifications
Sample : Whole heparin blood, Serum, Plasma,

Cerebrospinal fluid & Diluted Urine
Sample size : 130 micro liters of Whole heparin blood, Serum,

Cerebrospinal fluid, Plasma & 500 micro liters
of diluted (1:5) Urine
Method : Direct Measurement by Ion Selective Electrode

(ISE)
Detection Range : Blood
Na+ : 20.0 – 250.0 mmol/L

K+ : 0.2 – 40.0 mmol/L
iCa++ : 0 – 10.0 mmol/L
Cl- : 20 – 200.0 mmol/L
Li+ : 0 – 5.0 mmol/L
pH : 5.0 – 9.0
Urine
Na+ : 35 - 1000 mmol/L

K+ : 50 - 200 mmol/L
Cl- : 25 - 500 mmol/L
Serum and Plasma

Na+ : 20.0 – 250.0 mmol/L
K+ : 0.2 – 40.0 mmol/L
iCa ++
: 0 – 10.0 mmol/L
Cl- : 20 – 200.0 mmol/L
74
Li+ : 0 – 5.0 mmol/L

pH : 5.0 – 9.0
If iCa unit is in mg\dL, then the measurement range is 0-40 mg\dL.
Reproducibility (serum) Performed according to a modified NCCLS protocol EP5-T2
Reproducibility (expected) C.V. <1% C.V. <2% C.V. <2%

Day to day (n=20) C.V. <2% C.V. <2.5% C.V. < +- 0.03
URINE
Reproducibility Na+ : 35 – 1000 mmol/L
(Urine STD.) K+ : 50 – 200 mmol/L
Cl- : 25 – 500 mmol/L
Reproducibility (expected)
C.V<5% C.V<5% C.V<5%
Day to day (n=20)
Analysis Time : 65 sec. (Blood) / 80 sec. (Urine)
Data Storage : 1 Lakh Patient results, 25000 Urine results

100 QC results.
Calibration : Automatic or on Demand.
Output : 128x64 Graphics Display With Y/N

Numeric Keypad 24 column thermal printer
Ambient Conditions : 10-37°C (60-90°F), <85% humidity.
Power : 100/115~VAC, 50-60 Hz, or 220~VAC.
Size & Weight : 15” W X 12” H X 7.0” D, 10kg.
75
TROUBLESHOOTING
8. Troubleshooting
Introduction
To enhance trouble-free operation of the ST-200 PLUS (5 Ch), it is
imperative to follow the maintenance schedule outlined under ST-200
PLUS (5 Ch) maintenance.
When any error messages appear in the display, then refer to the list of
“typical error messages” to locate the problem area and proceed to the
appropriate section for troubleshooting instructions.
When performing troubleshooting procedures, put the analyzer in

MAINTAINANCE mode.
After removing the electrodes, solutions pack or after troubleshooting or

performing maintenance, the operator must do reagent priming before
calibrating the analyzer. To do this, Proceed to the following procedure.
Menu
Diagnostics
Flow
Reagent priming
Prime all
76
When reagent priming is completed, perform calibration by pressing

CALIBRATE Key.
Warning: Human body fluid specimens may be contaminated with HIV or

other pathogens.
Treat all specimens, collection devices and analyzer components that may
come in contact with specimens as biohazards.
If the appropriate troubleshooting procedures do not correct the observed

error, contact your ST-200 PLUS (5 Ch) distributors.
Troubleshooting Kit
Tools to help diagnose and correct problems:
 10mL syringe - 01
 Needles - 03
 Tygon Tube - 01
 Test Tubes - 10
 Spring Needle - 02
 L-Key - 01
 BD Disposable (5 Ch) - 01
 Fuse (2 amp) - 01
 Fuse (5 amp) - 01
 Disposable valve module part - 02
 Electrode seals
77
A. Flow Path System
All reagents and samples flow in one direction through the entire flow path
system as shown. The flow path is a closed loop which ensures that the
electrodes will always be kept wet, an important requirement of ISE’s. All
flow path connections are simple push-on, air tight connections, which
requires no special tools.
78
Figure 18
If removal of ST-200 PLUS (5 Ch) components is required during
troubleshooting, the component removal order should be as follows:
1) Electrode housing
2) Sampler (probe mechanism).
3) Valve module.
79
For example, if any electrode requires replacement, it is necessary to go in

MAINTAINANCE MENU for disconnecting the bubble detector tube from
sampler and pump tube from the electrode housing. Then carefully remove
the electrode housing.
If the solutions valve requires cleaning, the operator must remove all of the
items in the above list, before removing the solutions valve.
To install components on the ST-200 PLUS (5 Ch), follow the installation
order outlined under installation and replacement.
Troubleshooting is covered in the order that problems are most likely to

occur. Approach troubleshooting as a logical sequence of events. Isolate
the problematic area to avoid unnecessary component replacement and/or
excessive downtime.
ERROR MESSAGES their CAUSE and ACTION
The following actions have to be done when problem occurs
Error Messages Cause Action
Liquid is there in sample

detector or Sample Clean the bubble detector
Air Not Detected
detector is not connected and recheck.
properly.
Occurs at 80% pack
Replace Pack usage. Pack must be Check reagent pack.
Replaced
Liquid is not present in Clean the bubble detector
Liquid Not Detected
sample detector. and recheck..
Pack Expired
Reagent pack expired Check date & time
Check RFID connection /

Reagent pack is not
Invalid pack take pack from authorized
supporting
distributor
Sample not aspirated or
Insufficient sample is Aspirate sufficient clear
Air in Sample aspirated or sample is not sample before 60 sec.
aspirated in 60 sec. or Also check pump tube
hazy sample is aspirated.
RFID system in not Check RFID connectors or
Connection error
working. replace RFID board.
Cleaning solution is not Aspirate sufficient daily

Air in Cleaner
detected correctly. cleaning solution.
80
Do prime CAL A check

CAL A solution is not
Air In CAL A whether liquid is coming
detected correctly.
out or not
Do prime CAL B check
CAL B solution is not
Air In CAL B whether liquid is coming
detected correctly.
out or not
milli Volts of Na electrode
Drift Na differ from previous sample Calibrate
analyses milli Volts.
milli Volts of K electrode
Drift K differ from previous sample Calibrate
analyses milli Volts.
Drift Cl milli Volts of K electrode
differ from previous sample Calibrate
Same for iCa, Li, pH analyses milli Volts.
Bubble at Na Bubble is present at Na

electrode Check the flow path
Same for K, Cl, iCa, Li, pH
Bubble at Na
Bubble at K Bubble is present at Knock membrane part of
Bubble at iCa membrane part of Reference reference housing.
Bubble at Li housing
Bubble at pH
Depends on selected model

if instrument shows bubble
at all the parameters
B. Flow Errors
Obstructions or air leaks in the flow path will result in error messages
such as AIR IN CAL A, AIR IN CAL B, AIR IN SAMPLE, DRIFT and NOISE
These messages indicate flow problems. A low or empty reagent pack may
also cause AIR IN CAL A or AIR IN CAL B messages. If this problem exists,
81
based on usage since the last reagent pack installation refer to reagent
pack troubleshooting before proceeding.
If AIR IN CAL B or LIQUID NOT DETECTED! Message appears during a

SOLUTION PRIMING or calibration, and reassembled, check to make sure
that the electrodes are installed right side up and that all electrode
connectors are properly installed. Performing this function twice may
eliminate the error.
FLOW ERROR (Valve fluidic error or Fluid path error): Flow error depends on
following points.
1) Electrode housing
2) Sampler i.e. probe mechanism.
3) Valve module.
4) Reagent pack.
SOLUTION:
1) Electrode housing:
A) Remove sample detector tube from probe and check liquid flow as
follows:
Menu
Press 1 ↓
Diagnostics
Press 2
↓
Maintenance
The pump rotates. The liquid is aspirated through the tube. If liquid is
aspirated properly go to 2nd step Sampler
OR
If it does not aspirate properly, remove the electrode housing and clean
deposited protein from chloride electrode with maintenance needle and
reassembled electrode housing carefully.
Press “N” to maintenance exit. The pump rotates. The liquid is aspirated
through the sample detector tube to check either liquid is flowing properly
or not. If liquid is not flowing properly, then check PUMP TUBE as follows:
Menu
Press 1
↓
Diagnostics
82
Press 2
↓
Maintenance
The pump rotates. The liquid is aspirated through the pump tube which is
removed from electrode housing & check whether liquid is flowing properly
or not. If not, replace pump tubing.
2) Sampler (i.e) probe mechanism
Menu
Press 1
↓
Diagnostics
Press 5
↓
Hardware
Press 4
↓
Sampler
Perform sampler test. Check sampler hole, whether it is facing towards or

away from the operator. And if it is in the middle of valve module interface
seals. The sampler hole should always face opposite from the operator.
Align it properly by removing sampler from sampler holder and adjust

properly. Also check seals after 6 months or 2000 samples. If required,
replace it.
If Sampler procedure is acceptable, then go to the next step.
3) Valve modules
Menu
Press 1
↓
83
Diagnostics
Press 3
↓
SENSORS
↓
SENSOR CAL A- mV
SENSOR CAL B- mV
If it displays air in CAL-A or CAL-B or Bubbles detected in flow-path then

change or replace the disposable valve module as shown in below.
CAL-B tube
CAL-A tube
Disposable
Valve Module
4) Reagent packs
Menu
↓
Press 1
Diagnostics
84
Press 6
↓
Reagent Pack
(Check complete information & ID of reagent pack)

Finally perform reagent priming test as follows:
Menu
Press 1 ↓
Diagnostics
Press 4 ↓
Flow
Press 1 ↓
Reagent priming
Press 3 ↓
Prime All
D) After priming, proceed for Calibration and check the

“Calibration Slope” If it is in the following range
Na+ : Should be in between 80 to 40 mV/ decade

K+ : Should be in between 80 to 40 mV/ decade
iCa++ : Should be in between 50 to 15 mV/ decade
Cl- : Should be in between 80 to 40 mV/ decade
Li+ : Should be in between 80 to 40 mV/ decade
pH : Should be in between 80 to 40 mV/ decade
Then go for sample analysis or repeat calibration. After multiple

calibration failure, replace the faulty electrode.
C. Electrode Assembly
The electrode assembly should be replaced after 6 months of use.

Sensacore guarantees this item’s performance for 6 months of use, but the
frequency of replacement may depend on your sample volume or sample
type (volume of lipemic high protein samples may necessitate more
85
frequent changes of the electrode assembly). ST-200 PLUS (5 Ch)

customers testing 100 or more patient samples per week should replace
the electrode assembly after 6 months of use.
The electrode assembly should be checked to prevent any leakage. At the

same time, the electrode pins and pogo pins should be inspected to make
sure they are dry and clean, including the reference electrode pin and
Female connector, if low or erratic readings occur.
Electrodes with low calibration values (below 40 for Na/K/Li, below 15 for
iCa & below 40 for Cl), Drift, or Noise messages may indicate electrode
failure. Electrodes with HIGH calibration values messages most often
indicate an electrode assembly/disposable reference electrode failure.
For Na/K/iCa/Cl/Li/pH analyzer, when the electrode housing is removed

for electrode replacement, maintenance, troubleshooting, etc, wait for 10
minutes after reinstallation to ensure proper electrode temperature.
A Calibration values (Slopes) of the electrodes should fall within a range

of 80 to 40 for Na/K/Li/pH, 50 to 15 for iCa & 80 to 40 For Cl .If
they fall outside of this range, the out-of-range values * for “Na”,
“pH”, “K”, “iCa”, “Cl”, “Li” will be flagged. The analyzer should be
recalibrated. At the end of the calibration cycle, the slope values will
be displayed for each electrode. The ST-200 PLUS (5 Ch) will permit
sample analysis only for the electrodes that are calibrated
successfully.
If Na or iCa or both Na and iCa not calibrated, Li won’t analyzed,
because Li Electrode depends on Na and iCa Electrodes.
B A Na LOW, DRIFT, or NOISE message indicates that an additional

prime cycle may be necessary to condition the sodium electrode.
C A K LOW, DRIFT, or NOISE message may indicate that the

potassium electrode needs replacing. Before doing so, perform a
daily cleaning cycle, as described under DAILY CLEANER. Next,
perform a calibration. If the potassium calibration value remains
low, replace the potassium electrode. Do not clean the potassium
electrode with the sodium electrode brush tool.
D A LOW, DRIFT, or NOISE message for chloride or Ionized Calcium

only, may indicate that replacement of the specific electrode is
necessary. Before doing so, try performing a daily Cleaning, as
described under DAILY CLEANER?/DAILY RINSE?. Next, perform a
calibration. If the calibration value remains low, replace the
electrode.
E If all electrodes have low or high calibration values, the problem is

most likely with the electrode assembly or the reference electrode (on
the Na/K/iCa/Cl/Li/pH analyzer, the problem may be the
86
disposable reference electrode). It is also possible that an incorrect

reagent pack is being used, the ST-200 PLUS (5 Ch) will not
calibrate successfully.
F If drift or noise messages appear for all electrodes, there is most

likely a flow problem (refer to flow problems) or electrode assembly
problem (refer to electrode assembly). Also, inspect the electrode
pins, including the reference electrode pin, to ensure that they are
dry and clean. After reassembly, the analyzer should be primed and
calibrated.
Do not perform the cleaning procedure described in paragraph G as

a preventative measure. Do not clean the electrodes, as described, if
their cal values are within the acceptable range. Do not clean the
potassium electrode with the sodium electrode brush tool under any
circumstances.
G In some cases, the chloride electrode calibration value may drop

prematurely due to protein build-up on the inside surface of the
chloride electrode. Attempt protein removal by pushing the brush
tool (found in the troubleshooting Kit) through the chloride electrode.
Gently pull the brush back out of the electrode. Reinstall the
electrode, perform DAILY CLEANER and then CALIBRATE. If the
chloride electrode slope value remains low, install a new chloride
electrode. Do not use the chloride brush to clean the sodium and
potassium electrodes.
D. Reagent Pack
AIR IN CAL A, AIR IN CAL B and REAGENT PACK LOW messages may
indicate that the reagent pack is empty or low.
87
The ST-200 PLUS (5 Ch) has an RFID (Radio Frequency Identification

System) which keeps track of the reagent pack usage. The ST-200 PLUS
(5 Ch) will indicate the availability of reagents in a decreasing manner
i.e., 100% to 0% at every analysis.
When 20% of the reagent pack has remained in the reagent pack,
instrument will indicate the percentage till it reaches 5%. Below 5% the
instrument will displays CAL A INSUFFICIENT in blood and QC type
and CAL B INSUFFICIENT in urine type.
E. Bubble Detector
The bubble detector detects the difference between liquid and air, which
is important for proper sample positioning in the ST-200 PLUS (5 Ch).
To verify performance, proceed to *DIAGNOSTICS* followed by sub
menu HARDWARE and perform SAMPLE DETECTOR test.
In sample detector test, if liquid or air is not detected, replace bubble

detector.
F. Printer
If the ST-200 PLUS (5 Ch) printer fails to print, attempt the following
procedures:
1. To verify that the printer is turned on, enter the SETUP MENU and
check PRINTER ON/OFF.
2. Check the printer mechanism for any paper obstructions in the

printer head area. Remove any obstructions if possible, without
damaging the printer assembly or paper tear bar.
3. If steps 1 and 2 are successful, but the printer remains inactive,

remove the analyzer power cord for ten seconds, and then reinstall
the power cord. Press the paper advance button. The printer head
should move across the paper and the paper should advance
forward.
If the printer continues to function abnormally, contact your

ST-200 PLUS (5 Ch) distributor.
Performance Verification
Periodically, the laboratory may want to verify the analyzer’s performance.

Sensacore recommends the following procedures:
CALIBRATION – Automated
 Press “Y” to SYSTEM NOT CALIBRATED PLEASE CALIBRATE.

 Calibrant B and Calibrant A from the solutions pack are aspirated
and measured internally.
88
 ANALYZE SAMPLE indicates a successful calibration. If an error is

displayed, refer to instructions in the troubleshooting section.
ACCURACY
1. Calibrate the analyzer.

2. Using the quality control kit, test each control level three times and
record the data. Repeat this sequence for a total of 3 days.
3. Refer to the published ranges in the “Expected Value Chart”.
4. The mean value (each day) for each parameter should fall within the
published ranges.
PRECISION
1 Collect 5 patient samples.

2 Analyze each sample 10 times consecutively on the ST-200 PLUS (5
Ch) and record the data.
3 Calculate the mean, standard deviation and C.V. for each sample.
4 Compare the C.V. for sodium, potassium and chloride and the S.D.
to specifications.
CORRELATION
Use the ST-200 PLUS (5 Ch) and a reference analyzer (ISE, flame
photometer or other) in the laboratory.
1 Collect 20 patient samples (serum or plasma is preferable for

Na/K/iCa/Cl/Li/pH) and split in half.
2 Measure each sample on the ST-200 PLUS (5 Ch) and the reference
analyzer.
3 Calculate the difference (Reference analyzer value – ST-200 PLUS (5
Ch) value) for each sample and record.
4 The laboratory director should determine acceptability.
DISPLAY MESSAGES
Display Messages
89
ANALYZE SAMPLE Perform Whole heparin blood, serum &

CSF Sample analysis.
ANALYZE URINE Perform diluted urine Sample analysis.
ANALYZE QC Perform QC sample analysis.
AIR DETECTED Air detected in Sampler detector test.
AIR NOT DETECTED Air not detected in Sampler detector test.
AIR IN CLEANER Cleaning solution not detected correctly.
AIR IN SAMPLE Insufficient sample.
AIR IN CAL A Cal A not detected correctly.
AIR IN CAL B Cal B not detected correctly.
ASPIRATING Sample is being drawn into probe.
AUTO STANDBY Auto standby on/ off
CALIBRATION FREQUENCY Set auto-calibration time.
SIPPING FREQUENCY Set auto-sipping time.
CALIBRATION SLOPE Allows operator to display last calibration

values.
CALIBRATE Allows operator to calibrate analyzer.
CALIBRATING Analyzer is calibrating.
CAL A CONCENTRATION CAL A concentration values will be

displayed.
CAL B CONCENTRATION CAL B concentration values will be

displayed.
SOLUTION LEFT Percentage of reagent consumed
INCOMPATIBLE PACK Pack is not suitable for the instrument.
CLEANER SAMPLE
CLEANING… Analyzer is performing daily cleaning
90
CLEANING CYCLE PERFORMED … Appears after daily cleaner process over.
INSTRUMENT IS NOW IN
UNCALIBRATED STATE
Na/K/iCa/Cl/Li /pH HIGH… Na/K/iCa/Cl/Li /pH result obtained

is higher than the preset normal range
Na/K/iCa/Cl/Li /pH LOW… Na/K/iCa/Cl/Li /pH result obtained is

lower than the preset normal range
DATE/TIME Allows operator to change date and time
DAILY CLEANER Allows operator to perform daily cleaning
DIAGNOSTICS Allows operator to check key analyzer

functions.
DISTRIBUTOR ID: Allows the operator to check

distributor ID
DRIFT Na/K/iCa/Cl/Li /pH Na/K/iCa/Cl/Li /pH mV drifting from

First analysis.
ENTERING STANDBY Analyzer will go into standby mode in

60 sec.
EXIT- ANALYZE SAMPLE Sample not detected correctly

AIR IN SAMPLE Sample not detected
PRESS ‘N’ TO EXIT
PACK EXPIRED Check the expiry date of reagent pack
EXIT CALIBRATION Exit from calibration
EXIT DAILY CLEANING Exit from daily cleaning
FLOW To get the pump count and reagent flow

INSTALLED ON: Check the installation date
INVALID PACK Message will appear when the reagent
pack ID is invalid.
LEVEL 1 Allows the operator to select the desired

LEVEL 2 QC level analysis
LEVEL 3
91
LIQUID DETECTED Message will appear when the liquid is

detected in sample detector test.
LIQUID NOT DETECTED Message will appear when the liquid is

not detected in sample detector test.
LOT NO: Allows the operator to check lot number
MENU Menu selection.
MAINTENANCE… Instrument is in maintenance mode.
Na/K/iCa/Cl/Li /pH Appears when power is connected or

restored after Interruption
OEM/NON OEM: Allows the operator to check the

OEM/NON OEM
POSITIONING SAMPLE Message will appear during sample

positioning.
PROBE IN SAMPLE? Requests operator to confirm that probe is

PRESS ‘Y’ TO PROCEED in blood.
PRESS ‘N’ TO EXIT
PLEASE INSERT PACK Message will appear when the system has
not detected reagent pack.
PRIMING CAL A CAL A fluid line is being primed.
PRIMING CAL B CAL B fluid line is being primed.
PRIMING ALL CAL A & CAL B fluid line is being primed
PRINTER Allows operator to turn printer function on or

off.
PRINT- mV Allows operator to select if mV is selected.
The mV readings will get flagged.
PRINT STORED RESULTS Allows operator to display last sample
results or print stored results
PROBE IS MOVING FOR Message will appear while the probe

ASPIRATION POSITION is in aspiration position.
PROBE IS MOVING FOR Message will appear while the probe

REST POSITION is going back to rest position.
92
PROBE IN SAMPLE Requests operator to confirm that probe

is in sample.
PROBE IN CLEANER Requests operator to confirm that probe

is in Daily Cleaning Solution
PUMPING AIR Message will appear when air pumps
PUMPING CAL A Message will appear when CAL A reagent

gets pumped
PUMPING CAL B Message will appear when CAL B reagent

gets pumped
PUMP CAL A TEST Allows operator to test pump CAL A

calibration.
PUMP CAL B TEST Allows operator to test pump CAL B

calibration.
PUMP CAL ERROR Failure of Pump cal Test.
PUMPING LIQUID Message will appear when liquid is

pumped
PUMP SPEED = FAST Allows the operator to set the pumping

speed fast
PUMP SPEED = SLOW Allows the operator to set the pumping

speed slow
PRINT STORED RESULTS Allows operator to display or print

stored results.
REAGENT CONSUMPTION Allows the operator to check the quantity

of the reagent solution consumed
REAGENT PACK Allows operator to check the reagent

pack details.
REAGENT PACK CONSUMPTION Allows operator to display % pack
expended
REGEANT PACK ID Allows the operator to check the pack ID
REAGENT PACK INFO Allows the operator to check the pack

information
93
REMOVE SAMPLE FROM PROBE Requests operator to remove sample from

probe.
RFID READ ERROR When there is an error in RFID reading
SAMPLE DILUTED (1:5) Requests operator to aspirate urine.
SAMPLE DETECTOR Allows operator to test sample detector.
SAMPLE DETECTOR TEST Message will appear during sample detect

test
NORMAL RANGES Used to set the Na/K/iCa/Cl/Li/pH

sample limits
ANALYZE TYPE Allows operator to select the sample type.

Eg: SAMPLE/ URINE / QC
SENSA CORE
ST-200 PLUS (5 Ch) The first displayed message.
SENSORS Allows the operator to display the mV

readings of particular reagent selected.
SENSOR CAL A –mV Allows the operator to display the mV

readings of CAL A reagent selected.
SENSOR CAL B –mV Allows the operator to display the mV

readings of CAL B reagent selected.
SYSTEM UNCALIBRATED This allows the operator to calibrate. If

PLEASE CALIBRATE… the system is not calibrated; this
message will get displayed on the screen.
STANDBY Appears when Analyzer is in Standby

mode.
TOTAL NO OF SAMPLES: Allows the operator to check the total

samples Performed
APPENDICES
Warranty
94
Warrants the electrolyte analyzer will be free of defects in material and

workmanship for a period of twelve months from the date of purchase,
except for those parts or materials which are consumed or expended in
normal use. In the case of consumables or expendables, the warranty time
period shall be usage time prescribed in the operator’s manual providing
such consumable or expendable is used prior to any “install by” or
“expiration date”. The warranty is only applicable for Sensacore under
normal operating conditions and maintained in accordance with the
instructions in the operator’s manual and when not having been subjected
to accident, alteration, or misuse. In the event of failure within the
warranty period, sensacore will repair or replace any Analyzer returned.
Sensacore reserves the right to ask for proof of date of purchase, such as
original invoice and / or maintenance records.
Representations and warranties made by any person including dealers,

representatives and employees, which are inconsistent or in conflict with,
or in addition to, the terms of this warranty shall not be binding upon
Sensacore unless in writing and signed by one of its officers.
* * * * * * *
95
Sensa Core Medical Instrumentation Pvt. Ltd.
Plot No:3, Export Promotion Industrial park, Pashamylaram, Medak (Dist),
Hyderabad - 502307, Telangana, INDIA,Tel: +08455 - 223400. www.sensacore.com

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ST-200 Plus

ST-200 PLUS (5 Ch)

SENSA CORE MEDICAL INSTRUMENTATION PVT. LTD.

Understanding the ST-200 PLUS (5 Ch) Electrolyte

c. Setup menu ….. 61

S/N Figure Figure no. Page no.

ST-200 PLUS (5 Ch) ELECTROLYTE ANALYZER

1. Understanding the ST-200 PLUS (5 Ch) Electrolyte Analyzer

The ST-200 PLUS (5 Ch) is an automated, microprocessor-controlled

B. Summary and Explanation

Many medication states the cause of temporary imbalance of the body’s

C. Operational Hazards and Precautions

Read the operator’s manual before setting up or operating the ST-200

Observe all warnings, notes and key information in this manual.

The ST-200 PLUS (5 Ch) electrolyte analyzer contains sensitive electronics

The environment should be as free as possible from dust, mechanical

ST-200 PLUS (5 Ch):

To install or replace ST-200 PLUS (5 Ch) electrolyte analyzer components,

Valve module is mounted in ST-200 PLUS (5 Ch) for the supply of

Removing sample probe

Fixing sample probe:

Reference Housing: Electrode Removal

d. Building the Electrode Stack:

Remove electrodes, seals and electrode housing from the packaging.

Electrode housing must be in place to ensure proper operation. Upon

Loading the Stack

e. Install the Tubing:

Connect the sample tube to the top of the sample probe.

Front View Pump Assembly Modules

Pump module installation procedure:

1. Module base: It is fixed to the chassis through a lock at the top of

f. Reagent Pack Installation:

Do not use expired reagent pack. The electrode should be exposed to

To remove or replace any ST-200 PLUS (5 Ch) components, restore the

 Place the instrument into Maintenance mode.

Cleared liquid from the tubing

 To clean the flow path:

1. Remove the electrode housing by disconnecting the sample tube

 To clean the electrodes:

The built-in printer will provide a permanent record of calibration and

Refer to the following precautions:

1. Use only the printer paper provided by electrolyte analyzer

4. Do not apply any lubricants, grease, or other materials to the

When the ST-200 PLUS (5 Ch) electrolyte analyzer displays SYSTEM

Diagram of Back view connections:

OPERATING THE ANALYZER

3. Operating the Analyzer

The instrument has to be calibrated when it is switched ON or it

Following is the calibration procedure:

Switch ON the instrument

Na* K* iCa* Cl* pH*

PUMP CAL A TEST

Displays CAL B milli volts on display

Displays CAL A milli Volts on display

To obtain patient’s sample, follow the procedure and precautions described

After calibration, press Analyze key

Sample is positioned into the sensors

Displays given SAMPLE milli Volts on display.

Displays CAL A milli Volts on display.

* STANDBY *