1116005I Rev. 02
1116005I Rev. 02
1116005I Rev. 02
REF 1116005
2 x 50 mL CHLORIDE
THIOCYANATE
CONTENTS
Colorimetric method
R1. Reagent 2 x 50 mL
CAL. Standard 1 x 3 mL ENDPOINT
For in vitro diagnostic use only
PRINCIPLE INTERFERENCES
Chloride ions in the sample quantitatively displaces thiocyanate Lipemia (intralipid 1 g/L) may affect the results.
from mercuric thiocyanate. Liberated thiocyanate ion reacts with Bilirubin (40 mg/dL) does not interfere.
ferric ion forming a red ferric-thiocyanate complex proportional to Hemoglobin (12 g/L) does not interfere.
the concentration of chloride present in the sample.1,2 Other drugs and substances may interfere3.
QUALITY SYSTEM CERTIFIED LINEAR CHEMICALS, S.L.U. Joaquim Costa 18 2ª planta. 08390 Montgat (Barcelona) SPAIN
ISO 9001 ISO 13485 Telf. (+34) 934 694 990; E-mail: [email protected] ; website: www.linear.es NIF-VAT:B60485687
Samples with concentrations higher than 125 mEq/L (125 mmol/L) - Sensitivity : 0.005A / mmol/L chloride.
should be diluted 1:2 with distilled water and assayed again. Multiply
the results by 2. - Correlation: This assay (y) was compared with a similar
Concentrations less than 75 mmol/L, should be brought within linear commercial method (x). The results were:
range by increasing the amount of serum used. N = 20 r = 0.993 y = 1.05x - 3.1
The analytical performances have been generated using on
REFERENCE VALUES2 automatic instrument. Results may vary depending on the
instrument.
CLINICAL SIGNIFICANCE 1. Schoenfeld R.G., and Lewellen Clin. Chem. 10 : 533 (1964).
2. Tietz. N.W. Clinical Guide to Laboratory Tests, 3rd Edition. W.B.
Sodium and chloride represent the majority of the osmotically active Saunders Co. Philadelphia, PA. (1995).
constituents of plasma. As a result, chloride is significantly involved 3. Young DS. Effects of drugs on clinical laboratory tests, 5th ed.
in maintenance of water distribution, osmotic pressure, and anion- AACC Press, 2000.
cation balance in the extracellular fluid compartment.
Hypochloremia (decreased plasma Cl- concentration) is observed in
salt-losing nephritis as associated with chronic pyelonephritis. In
Addison´s disease, Cl- levels as well as Na+ levels may drop
significantly during the Addisonian crisis and in certain types of
metabolic acidosis (e.g., diabetic ketoacidosis and renal failure) and
aldosteronism. In metabolic alkalosis, plasma levels of Cl- tend to
fall while HCO3- levels increase.
Hyperchloremia (increased plasma Cl- concentration) occurs with
dehydration, renal tubular acidosis, diabetes insipidus, acute renal
failure, adrenocortical hyperfunction and metabolic acidosis.
Extremely high dietary in take of salt and overtreatment with saline
solutions are also causes of hyperchloremia.
ANALYTICAL PERFORMANCE
QUALITY SYSTEM CERTIFIED LINEAR CHEMICALS, S.L.U. Joaquim Costa 18 2ª planta. 08390 Montgat (Barcelona) SPAIN
ISO 9001 ISO 13485 Telf. (+34) 934 694 990; E-mail: [email protected] ; website: www.linear.es NIF-VAT:B60485687