Review

Methodological approach to the first and second lactate

threshold in incremental cardiopulmonary exercise testing
Ronald K. Bindera, Manfred Wonischb, Ugo Corrac, Alain Cohen-Solald,
Luc Vanheese, Hugo Sanera and Jean-Paul Schmida

a
Swiss Cardiovascular Centre Bern, Cardiovascular Prevention and Rehabilitation, Bern University Hospital
and University of Bern, Bern, Switzerland, bCenter for Cardiac Rehabilitation, Pensionsversicherungsanstalt,
St Radegund/Graz, Austria, cDivision of Cardiology, Salvatore Maugeri Foundation, IRCCS, Veruno (NO),
Italy, dDepartment of Cardiology, University Hospital Lariboisiere, Assistance Publique-Hopitaux de Paris,
Paris, France and eDepartment of Rehabilitation Sciences, K.U.Leuven (Catholic University Leuven),
Leuven, Belgium
Received 1 February 2008 Accepted 15 April 2008

Determination of an ‘anaerobic threshold’ plays an important role in the appreciation of an incremental cardiopulmonary
exercise test and describes prominent changes of blood lactate accumulation with increasing workload. Two lactate
thresholds are discerned during cardiopulmonary exercise testing and used for physical fitness estimation or training
prescription. A multitude of different terms are, however, found in the literature describing the two thresholds. Furthermore,
the term ‘anaerobic threshold’ is synonymously used for both, the ‘first’ and the ‘second’ lactate threshold, bearing a great
potential of confusion. The aim of this review is therefore to order terms, present threshold concepts, and describe
methods for lactate threshold determination using a three-phase model with reference to the historical and physiological
background to facilitate the practical application of the term ‘anaerobic threshold’. Eur J Cardiovasc Prev Rehabil 15:726–734
c 2008 The European Society of Cardiology

European Journal of Cardiovascular Prevention and Rehabilitation 2008, 15:726–734

Keywords: anaerobic threshold, blood lactate, exercise training, isocapnic buffering period, respiratory compensation point

Introduction The prescription of correct exercise intensity plays a

Exercise capacity is one of the most powerful predicting decisive role [5] and is either related to a percentage of
factors of life expectancy, both in patients with [1] and maximal exercise capacity, maximal heart rate or linked to
without cardiac disease [2]. Preventive strategies to avoid the concept of the so-called ‘lactate threshold’ (LT). For
development or progression of heart disease aim to the determination of the LT during exercise, basically
promote physical activity and to improve exercise two different methods are used: (i) direct invasive
performance. Prescribed exercise to reach these goals measurement of blood lactate (BL) or (ii) noninvasive
has to assure optimal efficacy and maximal safety, measurement of the ventilatory and gas exchange response
especially in patients with known cardiovascular diseases, with indirect definition of the LT. These differences in
where an exercise intensity above a critical level may have the methodological approach have led to a contradictory
potential detrimental effects [3]. Structured exercise- nomenclature and controversy on the physiological basis
based cardiac rehabilitation programmes have shown of what is called the ‘LT’ and even more confusion and
to be an effective approach to reduce mortality and misunderstandings prevail concerning the noninvasive
morbidity in these patients [4]. determination of the LT via changes of ventilation and
gas exchange parameters during exercise [6–8].
Correspondence to Jean-Paul Schmid, MD, Swiss Cardiovascular Centre Bern,
Cardiovascular Prevention and Rehabilitation; University Hospital, Inselspital, The aim of this review is therefore to order terms,
3010 Bern, Switzerland
Tel: + 41 31 632 89 72; fax: + 41 31 632 89 77;
threshold concepts, and methods using a three-phase
e-mail: [email protected] model [9].
1741-8267
c 2008 The European Society of Cardiology DOI: 10.1097/HJR.0b013e328304fed4
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 Determination of lactate thresholds Binder et al. 727

Fig. 1

Phase I Phase II Phase III

1st lactate threshold 2nd lactate threshold

„ „
“aerobic threshold “anaerobic threshold
Blood lactate concentration
40–60% of VO2max 60–90% of VO2max

Very light exercise Light exercise Moderate exercise Heavy exercise

Borg scale 6 − 9 Borg scale 10− 12 Borg scale 13–14 Borg scale > 14
45–55% of VO2max 55–70% of VO2max 70–80% of VO2max > 80% of VO2max
or HR reserve or HR reserve or HR reserve or HR reserve
60–70% HRmax 70–80% HRmax 80–90% HRmax > 90% HRmax
BL: < 2 mmol/l BL: 2.0 –3.0 mmol/l BL: 3.0–4.0 mmol/l BL: > 4.0 mmol/l

Percentage of VO2max

The three-phase model according to Skinner et al. [9]: relation between blood lactate concentration (BL) and exercise intensity. HR, heart rate;VO2,
oxygen consumption; Borg scale: subjective rating of perceived exertion.

Table 1 Threshold terms in a three-phase model of incremental exercise with a first and a second threshold of ventilatory/gas exchange,
blood lactate/pH or heart rate response, ordered by the date of publication
First (‘aerobic’) threshold Second (‘anaerobic’) threshold

Point of optimal ventilatory efficiency (PoW), Hollmann [14] Aerobic–anaerobic threshold, Mader et al. [15]
Anaerobic threshold, Wasserman and McIlroy [16]; Wasserman [17] Threshold of decompensated metabolic acidosis, Reinhard et al. [18]
Lactate threshold, Yoshida et al. [19]; Pendergast et al. [20] Anaerobic threshold, Kindermann et al. [21]; Skinner and McLellan [9]
Aerobic threshold, Kindermann et al. [21]; Skinner and McLellan [9] Onset of blood lactate accumulation, Sjödin and Jacobs [22]
Ventilatory anaerobic threshold, Orr et al. [23]; Simonton et al. [13] Respiratory compensation point, Beaver et al. [24]; Wasserman et al. [25]
Ventilatory threshold, Reybrouck et al. [26] Individual anaerobic threshold, Stegmann et al. [27]
Ventilatory break point, Brooks [6] Heart rate deflection point, Conconi et al. [28]
Lactate break point, Brooks [6] Second ventilatory threshold, McLellan [7]
First ventilatory threshold, McLellan [7] Maximal lactate steady state, Heck et al. [29]
Gas exchange threshold, Yoshida et al. [30] Terminal hyperventilation point, Simonton et al. [13]
First lactate turn point, Hofmann et al. [31]; Pokan et al. [3] Ventilatory threshold 2, Ahmaidi et al. [32]
Change point in VO2, Zoladz et al. [33] Heart rate threshold, Hofmann et al. [34]
Aerobic gas exchange threshold, Meyer et al. [35] Heart rate turn point, Hofmann et al. [31]; Pokan et al. [36]
Aerobic lactate threshold, Meyer et al. [35] Second lactate turn point, Hofmann et al. [31]; Pokan et al. [3]
Critical lactate clearance point, Brooks [37]
Anaerobic gas exchange threshold, Meyer et al. [35]
Anaerobic lactate threshold, Meyer et al. [35]

Energy supply and lactic acid production According to Skinner and McLellan [9], it is suggested,
during incremental exercise that the first threshold is called the ‘aerobic threshold’
The rise of BL concentration during stepwise increase of and the second the ‘anaerobic threshold’.
workload is best described using a model with two
turning points instead of continuous [10] or single Phase I
breakpoint models [11]. A number of studies have During the first phase of energy supply, greater oxygen
identified the existence of two ventilatory thresholds or extraction by the tissues is found resulting in a lower fraction
LTs during exercise to exhaustion. From the physiological of oxygen (PETO2) in the expired air. On the opposite side,
point of view, three phases of energy supply and two more carbon dioxide (CO2) is produced and expired.
intersection points can be defined with increasing Therefore, a linear increase in oxygen consumption (VO2),
exercise intensity (Fig. 1) [3,9,12,13]. Numerous terms CO2 output and ventilation (VE) is found. Increasing
have been described for an early (first) threshold and a workload during the first phase of energy supply does not
late (second) threshold (Table 1) [3,6,7,9,13,14–37]. lead to a significant increase of the BL concentration.

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 728 European Journal of Cardiovascular Prevention and Rehabilitation 2008, Vol 15 No 6

Phase II scientific world. Moreover, the initial concept of the

With increasing exercise intensity above a first LT the anaerobic threshold as a demarcation of the work load
lactate production rate is higher than the metabolizing (WL) above which the contracting muscles are not
capacity in the muscle cell. Lactate will appear in the adequately supplied with oxygen [17], has been notice-
compartment of the blood and leads to an increase in the ably challenged [6]. Concerning BL kinetics, in the 1970s
BL concentration. The concomitant increase in H + is Mader [29,15] introduced the ‘4 mmol/l LT’ and in 1981
buffered by bicarbonate (HCO3– ) resulting in an in- Sjödin and Jacobs [22] proposed the term ‘onset of BL
creased production of CO2 and a continuous rise in the accumulation’. Consequently, numerous other threshold
CO2 fraction of the expired air (PETCO2). Centrally denominations have appeared in the literature, which may
stimulated receptors integrating peripheral muscle and be allocated to the first or second threshold (Table 1).
chemoreceptor afferences lead to a steeper increase of VE,
whereas the increase of VO2 remains linear with increasing Until today, no widely accepted agreement on a
workload. The oxidative capacity of the whole system nomenclature for these threshold concepts has been
(i.e. nonworking muscles, liver, ventricular muscle mass) settled for the following reasons:
is sufficiently high to scope with the incoming lactate.
1. At no time point does the incremental exercise energy
During steady-state exercise, this leads to an equilibrium supply seem exclusively aerobic or anaerobic [41],
in BL appearance and BL elimination. The highest so the terms themselves may be considered mis-
workload, which can be performed for about 30 min nomers [6]
without a systematic increase in the BL concentration, is 2. BL kinetics, ventilatory response and gas exchange
called ‘maximal lactate steady state’ (MLSS) and reflects patterns depend on exercise protocol (i.e. fast vs. slow
the gold standard for the determination of the second or WL increments, step vs. ramp protocol) [7], WL
‘anaerobic threshold’ [6,38,39]. characteristics (e.g. running, swimming, cycling,
rowing) [42] and source of blood sampling (venous,
Phase III capillary, arterial, arterialised) [8,10]
With further increase in the workload above a second LT, 3. Cause and effect relationship of invasive (e.g. lactate,
the muscular lactate production rate exceeds the pyruvate, pH, bicarbonate, epinephrine) and non-
systemic lactate elimination rate. This leads to an invasive (e.g. heart rate, ventilation, respiratory gas
exponential increase in the BL concentration during exchange) CPX parameters seems clear in some cases
incremental exercise and a steady increase of the BL but a good statistical correlation does not necessarily
concentration (no steady state) during a constant work- imply a firm physiological link.
load test. A further nonlinear increase in VCO2, and more
pronounced in VE, is observed. At this point, hyperventi- The three-phase model for the description of lactic acid
lation cannot compensate adequately the rise in H + and changes and the corresponding respiratory gas exchange
there is a drop in PETCO2. kinetics during an incremental exercise stress test are,
however, particularly useful for the understanding of
threshold determination. Additionally, the determination
Controversial terminology
of a first and a second threshold fits the needs of exercise
The first descriptions of threshold concepts during
prescription particularly well. Therefore, we propose to
cardiopulmonary exercise testing (CPX) arose from the
use the three-phase model with the terms ‘aerobic’ and
need for rating physical fitness during submaximal
‘anaerobic threshold’ or ‘first’ and ‘second LT’ in this
exercise tests and from the observation of parameters
setting.
with curvilinear slope patterns [8,40]. One of the first
thresholds in noninvasive CPX was the ‘point of optimal
ventilatory efficacy’ (phase I to II), presented by Different approaches for determination
Hollmann [14]. Wasserman and McIlroy [16] introduced of the first (‘aerobic’) and second (‘anaerobic’)
for the same turning point the term ‘threshold of threshold during incremental cardiopulmonary
anaerobic metabolism’, defined by an increase in the exercise test
respiratory gas exchange ratio (RER) accompanied by a In the following section, an overview of methods (Fig. 2)
decrease in HCO3– . After their publication in 1973 [17], and terms (Table 1) is presented. Table 2 summarises the
the term ‘anaerobic threshold’ became popular as an most useful methods for threshold determination.
identifier for the first LT. The same term was, however,
also used to describe the transition from phase II to III as Lactic acid versus workload BL concentration does not rise
proposed by Skinner and McLellan [9]. linearly with increasing WL, but shows a sudden sus-
tained increase over resting levels at a certain individual
The use of synonymous terms for the first and second exercise intensity. This first abrupt increase in BL [19]
transition points caused considerable confusion in the was widely used as a gold standard for noninvasive threshold

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 Determination of lactate thresholds Binder et al. 729

Fig. 2

(a) (b) (c)

Lactate VE RER

WL WL WL

(d) (e) (f)

VCO2 VCO2 VE/VO2

WL VO2 WL

(g) (h) (i)

PETO2 VO2 HR
II

WL WL WL

(j) (k) (l)

VE VE/VCO2 PETCO2

VCO2 WL WL

Schematic representation of different methods for determination of ‘lactate thresholds’ during incremental exercise testing, applying a three-phase
model with an first (‘aerobic’) and second (‘anaerobic’) threshold [9]. WL, work load, HR, heart rate.

determinations by ventilation or gas exchange para- Three-line regression concepts have incorporated the two
meters. A log–log transformation of BL versus VO2 [43] LTs into one graph [11]. The ‘second lactate turn point’
is a demonstrative way for the detection of this ‘first lactate was shown to correlate well with MLSS [38,39] and
turn point’, which may approximate BL levels around approximates a BL level of 4 mmol/l [29,15]. The
2 mmol/l [21]. Fixation to an absolute BL concentration, important point in favor of this threshold fixation,
however, is protocol dependent and it does not consider however, seems the convenience of a whole number.
interindividual variance in resting BL levels nor does it Defining an ‘individual anaerobic threshold’ possibly
reflect the LT as a flexion point [12](Fig. 2a). better reflects the individual lactate level at the MLSS,
which may significantly deviate from the 4 mmol/l level
With increasing WL above the first LT the patient [27,32].
reaches a point at which lactate production equals
maximal lactate clearance capacity. This was called the VE versus workload The curve of minute VE shows a
‘MLSS’ [29] or ‘onset of blood lactate accumulation’ [22]. curvilinear slope pattern with two break points. The first
It reflects the WL threshold beyond which endurance coincides with the ‘aerobic threshold’, the second with
exercise will not lead to a steady state and is used as the ‘anaerobic threshold’ [9,13]. By drawing a tangent
an upper limit of intensity during endurance training. from the zero point to the minute ventilation and

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 730 European Journal of Cardiovascular Prevention and Rehabilitation 2008, Vol 15 No 6

Table 2 Most practical methods for first (‘aerobic’) and second (‘anaerobic’) threshold determination
First (‘aerobic’) threshold Second (‘anaerobic’) threshold

Intersection of a two line regression of the VCO2 versus VO2 (V-slope) graph, with a change of the Inflection of VE versus VCO2 (respiratory
slope from less than one to greater than or equal to one (Figs 2e and 3a) compensation point) (Figs 2j and 4),
Nadir or first increase of VE /VO2 versus WL without a simultaneous increase in VE/VCO2 versus WL Nadir or nonlinear increase of VE/VCO2 versus WL
(Figs 2f and 3b) (Figs 2k and 3b)
Nadir or first rise of PETO2, while PETCO2 remains constant or is increasing (Figs 2g and 4b) Deflection point of the end tidal PETCO2
(Figs 2l and 4)

VCO2, carbondioxide consumption; PETO2, fraction of oxygen in the expired air; PETCO2, CO2-fraction of the expired air; VO2, oxygen consumption.

dropping a line on the abscissa (VO2) a ‘point of optimal WL [17] at the first threshold with a further increase at
ventilatory efficiency’ [14] was determined. The ‘point of the second threshold (Fig. 2d).
optimal ventilatory efficiency’ reflects the point at which
a maximum amount of oxygen can be taken up with VCO2 versus VO2 One of the most frequently used
a minimum of ventilation (Fig. 2b). In a multiline methods for the determination of the first threshold is
regression model [7,23] the first curvilinear rise in VE is the ‘V-slope method’ [24]. During the early WL
called the ‘(first) ventilatory threshold’ (VT1). It reflects an increments in CPX, VCO2 rises as a linear function of
increasing ventilatory drive because of excess CO2, stem- VO2, but as exercise intensity increases, a subsequent
ming from the buffering of lactic acid by bicarbonate. increase in this slope occurs (Figs 2e and 3a). Practically,
the VCO2 versus VO2 curve is divided into two regions
With increasing WL beyond ‘VT’ a second curvilinear fitted by a two-line regression with the threshold at the
rise in VE may be observed. This second increase in intersection. Besides the two-line regression model, the
ventilatory drive is also caused by increasing acidosis threshold in this graph may be fixed to the point where
and by additional CO2 stemming from lactic acid the slope changes from a value of less than 1 to Z 1 or
buffering [18]. The ‘second ventilatory threshold’ where a 45-degree (slope = 1) tangent touches the graph
(VT2) [7,32] is demonstrated by the second break point [47].
in the three-line regression of VE versus WL.
The V-slope method should not be referred to as the
‘VT’, however, because the VE is an equal factor on both,
Respiratory gas exchange ratio versus workload The RER is
the x-axis and y-axis, and does not account for the break
the ratio of CO2 output and O2 uptake (VCO2/VO2). With
point in the increase in the V-slope plot. The V-slope plot
steady-state conditions, the RER equals the respiratory
is a plot of increased moles of CO2 output relative to
quotient, which should be reserved for expressing events
moles of O2 uptake and the threshold result of the
at the tissue level. The steepest part of a curve plotting
addition of CO2 to the venous blood.
RER against VO2 was called the ‘threshold of anaerobic
metabolism’ [16] but the initial concept of the anaerobic
VE/VO2 versus workload (Fig. 2f ), PETO2 versus workload
threshold has been widely questioned [6] and the same
(Figs 2g and 4b): PETO2 decreases during the initial WL
term is also in use for the second threshold [9,21]
increments because of changes in the physiological dead
(Fig. 2c). The first threshold may also be defined at the
space and tidal volume ratio. At the first threshold, when
point where the RER versus WL curve having been flat or
VE increases out of proportion to VO2, PETO2 reaches a
rising slowly, changes to a more positive slope [24], with a
minimum and increases thereafter. In other words, the
value approaching, but remaining below 1.0 [44]. Fixation
VE/VO2 slope breaks with linearity and increases, whereas
of the threshold at RER = 1 or at RER Z 1 with sustained
the VE/VCO2 slope first decreases and subsequently
increase [45] renders conflicting correlations with other
remains constant (Fig. 2k) [18,24]. Correspondingly,
methods [13,46].
PETO2 is noted to increase, whereas PETCO2 does not
change (Fig. 2l) [17].
To the best of our knowledge, there is no validated
breakpoint in the multiline regression analysis of the RER As a three-line regression model of VE versus WL shows
versus WL, which was correlated with the second an inflection at the second threshold while VO2 remains
threshold. Fixing the second threshold at a RER of 1.0 linear, there is also a second inflection in VE/VO2 versus
or slightly higher reflects no breakpoint whatsoever. WL. This determination method, however, is barely
realised.
VCO2 versus workload The H + ions of rising BL are
buffered by bicarbonate causing an increase in CO2 VO2 versus workload VO2 rises linearly with WL. During a
produced from the rapid dissociation of carbonic acid. ramp protocol, no breakpoint may be detected, but in a
This is reflected by a curvilinear increase of VCO2 versus step protocol a delayed constant value may be observed

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 Determination of lactate thresholds Binder et al. 731

Fig. 3

(a) (b)
1500 50 40
V-slope method
1300 VE/VCO2

1100 40 30
VCO2 (ml/min)

900

VE/ VCO2
VE/ VO2
Slope > 1
700
30 VE/VO2 20
500

300
Slope < 1 20 10
100

200 400 600 800 1000

VO2 (ml/min) Work load

(a) Determination of the first (‘aerobic’) threshold by the ‘V-slope method’. (b) Determination of the first (‘aerobic’) threshold at the nadir of VE /VO2 and
the second (‘anaerobic’) threshold at the nadir of VE /VCO2.

Fig. 4

(a) (b)

VE/VCO2-slope 20 6.0
75
PETCO2 5.5
18
5.0
PETCO2 (kPa)
PETO2 (kPa)

50 16
VE (l/min)

4.5
14
4.0

25 PETO2
12 3.5

10 3.0

400 800 1200 1600 2000 Work load

VCO2 (ml/min)

(a) Determination of the second (‘anaerobic’) threshold at the inflection of the VE versus VCO2 slope (‘respiratory compensation point’). (b) Determination
of the first (‘aerobic’) threshold at the nadir of fraction of oxygen (PETO2) and the second (‘anaerobic’) threshold at the inflection of carbondioxide fraction
of the expired air (PETCO2).

beyond the early threshold [48 ](Fig. 2h). This phenom- ‘Heart rate deflection point’ [28]. This breakpoint occurs
enon was called ‘Change Point in VO2’ [33]; however, at the second threshold and not the first [50,36]
the precise physiological mechanism responsible for the (Fig. 2i). As this ‘heart rate turn point’ is not always
changing VO2 pattern remains to be established [49]. found, it may not be regarded as a generalizable
physiological variable. An upward flexion (I) may suggest
Heart rate versus workload A deflection from linearity of the a decline in left ventricular function [36], shown in most
heart rate versus WL curve may be detected, called the patients with coronary heart disease, whereas a deflection

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 732 European Journal of Cardiovascular Prevention and Rehabilitation 2008, Vol 15 No 6

(II) is rather a physiological phenomenon observed in Fig. 5

approximately 85% of healthy adults at the second
threshold [31].
20 6.0

VE versus VCO2 (Figs 2j and 4a), VE/VCO2 versus work load 5.5
18
(Fig. 2k), PETCO2 versus work load (Figs 2l and 4b): To PETO2
compensate for the decrease in blood pH beyond the 5.0

PETCO2 (kPa)
PETO2 (kPa)
16
second threshold, VE increases out of proportion to VCO2. 4.5
This inflection in the VE versus VCO2 slope has been
14
called ‘respiratory compensation point’ [24]. It correlates 4.0
with the second break point of the VE versus WL graph
12 PETCO2 3.5
(Fig. 2b). The point is thought to represent a relative
hyperventilation because of metabolic acidosis. This 3.0
10
threshold may be also determined by the minimal value
in the VE/VCO2 versus WL relation (Fig. 2k) [13]. When
no nadir is found, the threshold may be determined at the Work load
deflection point of PETCO2 versus WL (Fig. 2l and 4b).
Cardiopulmonary exercise testing of a 74-year- old man with ischaemic
cardiopathy and heart failure (left ventricular ejection fraction 20%),
Particularities of threshold determinations showing a lack of a nadir but rather a continuous rise of fraction of
oxygen (PETO2) and decrease of carbondioxide fraction of the expired
in different clinical entities air (PETCO2) from the early stages of work load increments, indicating
In patients with cardiovascular [51] or pulmonary [52] early onset of anaerobic metabolism. Furthermore, because of rapidly
developing dyspnoea, no respiratory compensation point is discerned.
disease, early onset of lactic acid accumulation during
CPX may impair accurate noninvasive determination
of the transition from phase I to II [52,53]. This is
manifested by the lack of a nadir but with rather a
continuous rise in the VE/VO2 (Figs 2f and 5) from the Fig. 6
early increments on. Consequently no inflection point of
the PETO2 (Figs 2g and 5) may be observed.
40 40
VE/VCO2
Patients with chronic heart failure will be more likely to
present with an abnormal ventilatory response [54] (e.g. 35 35
exercise oscillatory ventilation; Fig. 6) making threshold

VE/VCO2
determinations based on breathing patterns prone to
VE/VO2

inaccuracy. In addition, respiratory compensation for the 30 30

decrease in blood pH (phase II to III) may be affected
in severely dyspnoeic patients who, however, hardly ever 25 25
reach this exercise stage. VE/VO2

20 25
Likewise in patients with chronic obstructive pulmonary
disease, lactic acidosis may occur early during CPX [52].
Furthermore, because of rapidly developing dyspnoea, Work load
chronic obstructive pulmonary disease patients may
neither show a first (Figs 2b and 7) [55] nor a second Exercise oscillatory ventilation in a 61-year-old man with congestive
heart failure (dilated cardiomyopathy, left ventricular ejection fraction
LT [24] (Figs 2j and 7). 25%), precluding any reliable determination of any ventilatory threshold.

Patients with skeletal muscle mitochondrial disease may

exhibit elevated BL levels at rest or with minimal
exercise [56] associated with exaggerated ventilatory
response [57]. Decreased oxidative capacity of the phorylase), no metabolic acidosis will occur during CPX
affected mitochondria causes shortening of the aerobic– [58]. Consequently, VCO2 will not increase out of
anaerobic transition (phase II), making discrimination of proportion to VO2 and the RER will not rise above 1.0.
the first from the second threshold difficult (Fig. 8). The transition from phase I to II will hardly be
discernible. A curvilinear increase in ventilation and a
In patients with McArdle’s disease, who do not produce drop in PETCO2 may be observed because of hyperventi-
lactic acid during exercise because of the lack of the lation, possibly caused by nonhumoral stimuli originating
muscle isoform of glycogen phosphorylase (myophos- in the active muscles [58].

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 Determination of lactate thresholds Binder et al. 733

Fig. 7 istics. Determination of the first threshold and second

threshold, however, seems accurate [61].

VE/VCO2-slope
60 Summary
Threshold names and concepts describing the changes of
BL during CPX have been at the origin of extensive
controversy in the scientific world. The aim of this review
40
was to give an overview of the different concepts that
have been described during the last decades, to order
VE (l/min)

terms and to propose the three-phase model of lactic acid

accumulation with a first (‘aerobic’) and a second
(‘anaerobic’) threshold as the basis for further discussion.
20 The advantage of this concept is the clear differentiation
between two ‘LTs’ of different meaning and its applic-
ability to exercise prescription, especially in cardiac
patients with different degrees of functional impairment.
Caution, however, remains to be taken when reporting
about data measured at different ‘LTs’ to avoid mixing up
300 600 900 1200 1500
methods. As scientists may use the same terms for
VCO2 (ml/min)
different phenomena, it should only be used in combina-
tion with a statement about the method used for its
Cardiopulmonary exercise testing of a 61-year-old man with respiratory
exercise limitation because of severe obstructive pulmonary disease: determination.
no inflection (‘respiratory compensation point’) is discernible in the
VE /VCO2 slope.

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