p icrobiolosy Laboratory A.lntroduction to M Competencies il i i ious diseases and the MIl.1: Deseribe the different causative agents of Infectious s a sed in their detection, and discuss the role of microbes in health and disease thay agents of Infectious disease, MI 1.2: Perform and identify the different causative Gram Stain, ZN stain and stool routine microscoPY Objectives: Atthe end the session, the students shall be able to, «Describe the structure and functions of Discuss the general principles and infectious diseases (Emphasize on Koel Explain the Good laboratory practices standard precautions Demonstrate practice o! ept of Biosafety in the laboratory laborato: Specific Learning Microbiology ry it in the laboratory diagnosis q . . Explain the conc Exercise 1.A: 1, Enumerate various sections/divisions of Microbiology laboratory: describe their sts carried out in each section. functions and major diagnostic te Section Diagnostic tests done Direct debeckor 1 yar gpa , ace Fast stko , pibast 8KSn ask gourd Cath , antimicseblal Bacteriology ability te igen, leech op specie Prk y i Virology Duteclfon of val 2” molecals rmethodd fo clalect Visa! genat 1 Solan of-Vibus Tmlscscophe. ambration Enantrokion of blood + Pus Parasitology cok thes Sprains» Smmuncellagnes he methnoels ology Pacipshtion Kexltos, yg vation eachion, complesnerd eesay ELI As TFA THC, fasdion, neakaloahon fet, ial Rey) dst, cesta blob 1O§ ey Gram Shor , Kon Stet» Palla for » ler oni 8 Inctophanol Coon blu, hehopalholag ia ? shbr, Sep Golhut ; Molecular biology 122, Describe laboratory method utilized for demonstration of Koch’s postulates for establishing the causative role of a pathogen in a disease process with suitable example. 1 Micso cagenton shouted be cenklonl eastioted wait show pea nEee : 2 Fsotahor of ee 7p pu ple uncks misoscape Huts from bsfon | Gathuse disease) anfnn | 3 Sunt dieas must be dech Toewate Asal antral wr' ren Srocateted In lab eofinal | Dmnlate Melty | anfmnal 1 + uspecks| Fhe Slatin Pr pust Cesltese box a Lalor _produced ta anima! Gate 5 Antibody agains osganisrn must be preeat in Sesum Eusy 3, Discuss the significance of proper hand hygiene Hood hygfine is ont rncst Pmparbunt meas 4o prevent disease Gensmtesfon Hands au the maf Souc0e of Pnfection. 4, Enumerate the diseases that can be transmitted through contaminated hands Grabro-Fatahne Papechion - Sol monellests Reptralosy Systern Pafections iice- Znflema, (ovro =o Glel_efe. 5, Suggest the appropriate type of biosafety cabinets to be used in the following scenarios eNesontSmet, cleft osgansm, unlikely to Cauie dbease = Gat§nment; modish isk, lis case “fo varying | Fee = Hish conleSament rewscso/ Hansress ian, Baious / | bcay lethal clases Bt. Ss ; =) = max Contefament exohic ’ Hig h she geri | gsi 4 Aseatig lBease- B1B. Microscopy Specific Learning Objectives: At the end the session, the students shall be able to, pet Enumerate different type of microscopes : ; : Desoribe the working principle of compound microscope, Dark-field, Phase contrat, tt and Electron microscopes ae ye ahenn ne lications of various microscopes 1n diagnostic microbiology «Discuss the app © Focus the compound microscope under various magnification powers © Describe the concept of ‘Micrometry Handle the microscope with utmost care Exercisel.B: 1) Enumerate different types of ‘microscopes used in diagnostic microbiology laboratory it teh calisescope ii ) Dosis fold Ga Dasic -- 1 misoge it) Phase Conbrast crirsascape ¥%) Clewsosceone minsenpe 0} elechon enlesnscore. 2) Describe the principle and uses of various microscopes a) Dark-field microscope Principle: Oaske ffeid condenses Js used hat conhal opaqucaca bleckSng ligne bom anton objective lens really, hed annvlas hollew ata—s Wank falls on Speen obliquely 9 Brightly Luarfhated Specewen etl dose backepound Uses: To Seluntily (ing, Unslefaed cells and HE, backate Whe SProchact Prochastes hth Cannock be Visvalised by nt onl Soscepy 4— b) Phase contrast microscope Principle: Uses: 75 = Miebial nig objective lens —0 ocular lens 6 unstatne! ebfect appear dak Sn Loright taaclapound foamed ductor 8, ee Deleckicg |__ ea, Fnelusin beclfes- c) Fluorescent microscope us Wight rays pass Klsough 4 3 ugh 4 Concinses —? 4 pectenen 9 phase at Stucky Susceptibility + Detesreritng Shepe of cells Gaeiml Cellular Components ~ cell membrane Nude » Sp indla Principle: an fad bhost Wave Flousoscent chyes au Expac! to UV Rey Loy Woecone Exfted fo flowoscence Ue, oy Convest “this Bo visi be light tty length to longes Lave Length Cte, Visite Ugnt) Uses: ‘) ri eaisebes wits Hloswscent elyt— Ey» Accieline cb 2)imeunel whoscence — Tnaltaect and dined Immune Houtescen louroscence migacopy- Auto Howsescence~ Ex i- Cyclo spear e - Flased Nematoda 4 plasmedim — Actimine phinol — Tebsce basil? ce, Hest = Adc LE Plowso 3) Gn] : o. Electron microscope Principle: © have Wave lengtr 105 Kine Sheastey than photons ,hince EM uses accdsated CO 08 Souseeog ELlumPnahon, Uses: Vinal cubeckion - ) Dcckly fsom Specimen Extn 2) fom Gultuse 8) Altes adding Ankita antlocds Rolautaus proms + Contest of EW — by -ve dlefnig + Bhaclowoing Frecetng tlehfag feehnt pve 153) Label the parts of microscope in the given picture elas lens: —>= Refahing proms nese pdee i Hvelans a Couise, Mfjostmad > Skye. fiw adfesment = ——____y ——_ Unt Sousae 4) Suggest the type of microscope, desired magnification and method used in the following scenarios Stool examinati 7 parasitic eggs Migosope.| UO ~100% > Keb . techy Urine examination for pus 10; cells in a case of Urinary tract Compound 7 ne infection mifigascope- | UO skisiag 100%. Bacterial examination of CSF sample in ease of pyogenic | “7rd wa-rcoe | ah8bg meningitis nisescope Bacterial examination of tissue fluid from gummatous Ulcer in a case of sexually Compound {8% too Storing, transmitted disease Pricsoscapy. 165) Write the differences between Electron microscope and the Light/Compound microscope ompound Recliation Source, Paible Light Eleckon beam Ais, Hh _ Vaccum Giles 4 Eleclro magnet ©+2 sk 0.50 higst rmragrl colton tooo — Woo > (90, c06 Pogsllole. + Assessment 2 ‘ Participates actively and contributes to discussion during SGT 1 2 43 3 ‘Shows professional conduct during the Teaching Learning 2 session 4 ‘Completes the record book activities in time 1 {2 = 43 5 Shows evidence of learning the new skills 1 2 3 (itellectual/Psychomotor) Total score AS (Can be reduced to 5 for convenience) Faculty Remarks/Feedback: Date: Faculty Name & Signature # Mark as 1, 2, 3 for ‘Not satisfactory’, ‘satisfactory’ & ‘Very Good? respectively 7Exercise 21: 1) Suggest suitable clinical samples to be collected in the following infections/diseases caaneal Scraplap Conjuncti Confunchival Svabs Keratitis Endopthalmitis + Ageous (65) vitreous Huta’ Otitis media Swabs from cutis cos , As packs —_ [goer Ennus as Oral and dental infections Meningitis: CSF Skin and soft tissue infections ‘Aerobic - Pesces) Exodak , oand Swabs ‘Anaerobic Aspitcta, “W'ssua Specimen , blood : __ Gnd fei Upper respiratory tract infections scat Saks with membran eves Fonsi, (Pharyngitis, tonsillitis, diphtheria) Nasaphosinecal Sisal Lower respiratory tract infections Span,’ Erode! oS pPeat y (Bronchitis, Pneumonia, pulmonary ( spe tuberculosis) levee! leaves ey co (a) Septicemia Paked bleed Galtast Spelman. Collected asepHeatly Endocarditis 2 Bhep disenfechor of Sifa » 8- loms of Blobd: Diarthea, Dysentery, Food poisoning Stool, Reda) Sats Genital infections (Gonorthea, syphilis, |Usettsal Swabs, Givieal Scabs 7 Erudate chancroid etc.) Urinary tract infections [= Midstream ustne : k Codtebesizecl Patient Diseases requiring —Bloed tp Vacubtns. serological/immunological tests 2) Fans the precautions to be taken while collecting samples for anaerobic culture. 4 Special Ontaisle, bonspard fystemn one transposed Learaechitel, totic lab ZF —twith media Shrild be Urted.3) Enumerate the culture media and methods used for anaerobic culture. Anaciobié culture media ‘Anaerobic culture methods Anaerobic. A Evaluation ancl Replocemed | 3 Rebestson Cooked rent broth A Morvald Attersled 44, Sho, VWoglyectede Groth 14 Aelsoay ok Op I Anascbic Bled ages len ok 2 by cheniad relied [> Gren hsot infusion Aged © Crespck denen beg 0 Fag Yelk og [? Anasrcbje Gilovelox 4 Preto Weak gtobton I? Necmyen blood Agas > pany) tay ys [Ses age > Fre Read Arawicbicalty Btulirg) CPRag: 4) What are the criteria for sample rejection? olan wesess Semple St celeste Th RSeabed algo .D8en9fveuipaak meio 33 used, Sample is Refected ©, Olen 2 ‘ 5) What are the general principles to be followed for specimen collection? ] ‘3 be Nous C0 \leary ling all * Specir Pesimen held not be collected Iq Conkstint Conteinig foamelfy fer entsodidegey anal 209 Label the blank parts of the packed clinical specimen in the following picture as kiget pestic gecephacle Substance —alosonbant costtfieadion mask Shipp “dintikeation (Source: Center for Disease Control, Atlanta, USA) 7) Write the general guidelines for specimen transportation 1s Tease tubes = vestcally = cls Ga clot formation Bleed i +transpost| bags: Sd Avant closuss , Siig packet Joh Lue paps Doale : ae 2S ced (ds. 8) What are the important and essential components of specimen label? Tmnpalance = all Spec onen Should be labelled fo ensuse thot an acousethe cfacestls in béfag_sopatted ts cosaect poctfunt TDs label shold Paclete “i dame _,medfent sexes nambes_, der of Wilh dots of Collection SpeSmen Lime al Col ection. 2110) Enumerate the steps used in bacterial culture ww Waite the methods used for beta identification ion pee ak aa ey Spam aca Sith cia Bac “ist test. = Bulacnated Aystens Jor ackesial Teter cating MALDT To E = VTTEK <2 —__ Phen = _midoscan Dal Arcay Syston. 12) Write in brief on professional conduct ben ining to the clinical samples Cootidentialtly — patienb _prsonal bualth Jormatin Bnet enhanced wilh otlus. focludrag clase Aelalives 4 ernployee's 20 Hheut patter content = dofeitmed Content = Tha pati glvet iosfthen oscinfss ian tacos out tev petting andisshaodug the pootentia! Graplteahions » Sfoke advan tag op Hu ot fest gesuils? 7 a _CuntellPad ~ $s Coabidintlal Communfeabion Lifled fo tte patient and a _Quntel) od. he 23AETCOM Exercise Exercise 2.2: Demonstrate respect for patient samples Competency: MI 8.11 Demonstrate respect for patient samples sent to the laboratory for laboratory tests in the detection of microbial agents causing Infectious diseases Paton Specific Learning Objectives: At the end of the module students should be able to * Describe and apply professional and moral values related to patients? Clinical the sample reception and Processing stage Sap Identify the routine events in sample collection, transportation and disrespect to clinical sample, : "OTe that a Identify the ways to rectify them and demonstrate respect to Patients’ samples, Describe and demonstrate application of ethical principles and medico legal issues A laboratory results : In the given situation/case Scenario, identify the events that show disrespect / breach j confidentiality to the clinical sample/ data and Suggest ways to rectify them, " 1) Biopsy sample of a critical Patient getting rejected because it was collected in a formaly saline = Blep, of ° ese a eg ee pcs pce 6 a A Snad wae 42 over serum i wi ) Left st sample getting used for research without infc informed consent 3) Sample is accompanied with request fe it form with incomplete informati ion/ wrong patient credentials ‘We & x ropes pee u ' ! eosS 25Assessment 1 requisite prior knowledge 1h 2 Participates actively and contributes to discussion during SGT : 1 i 3 ‘Shows professional conduct during the Teaching Learning session 1 py 4 Completes the record book activities in time 1 R 5 Shows evidence of learning the new skills 1 P ‘lectual/Psychomotor) = = Total score | /15 (Can be reduced to 5 for convenience) Faculty Remarks/Feedback: oe Faculty Name & Signature # Mark as 1,2, 3 for ‘No satisfactory’, satisfactory & ‘Very Good! respectively 26 _Certifiable Skill Exercise Hand hygiene, PPE - Donning & Doffingof hand gloves — (1) Exercise 2.3: hand hygiene appropriately and in the correct sequence a Palm to palm Back to palm (both sides) = of finger on pal {Rotations rubbing, both sides) (both the sides) (Source: Essentials of Practical Microbiology, 2) Write examples of situations requiring hand hygiene in an out patient department 4 Ales dasifag cach, palferdy oka touclfag sett bast hands » Eni bru rent San bs = Conkfamestes bod + dof fous befess leaafin the ieatmert asta , 3) Perform donning and doffing of gloves appropriately Steps of donning (wearing) gloves, 27Steps of doffing (removal) gloves 4) Write examples of situations requiring use of gloves in an out-patient department = ini t: Behe 4 Abe cxomining tue pain Ss =—alun you touch bodfly [utes S Salant 2, Sa lab. 5) You are about to perform phlebotomy for serolo; procedure. Demonstrate the appropriate method Perform hand hygiene where ever gical testing. Choose appropriate PPE forth of donning and doffing of the selected PPE ca 4 Folds neodle 4 Sysiree os Vocwum dule dupernting naive is te J u 7 Pate beal inet has Gone Ask dhe plot to }fam Abt sosst = pat on iaeil_ filing gloves. Dialer! dia site = Archon tix yen a entes avin at ar ads ° on “once Zulli S to at blood i) collec Release dhe dnsefhad - Oihabos Here ~ ABcatd tee needle ~9 check tu label- dead Shasp 4 bretangls ~ Remove slows piose tam Pr, jou! wate 283, Direct methods of bacterial detection Competency + a att i i: v1 ie Perform and identify the different causative agents of Infectious diseases by Gram Stain specific Learning Objectives: ‘At the end the session, the students shall be able to, Enumerate various types of staining techniques used in bacterial identification Describe the principles of simple staining, hanging drop. Identify the different causative agents of Infectious diseases by simple staining & Describe the principle of Gram staining Perform Gram stain on the smear provided, record the observations with help of a colored labelled diagram and interpret the findings. «Identify different causative agents of infectious diseases by Gram Stain Exercise 3: 1) Record the findings of simple staining 2) Write the clinical applications of simple staining —tued _Jos dberrefrG9 Cll Shape, dire 4 pranagemenl~ Sh bee of bactesfe = Ge char abut tee Folenbfitation, sspecfally fos _gsam —ve baciilf 313) Record the findings of Hanging drop ts? Rachie, Slide unde (Ox —_ ° NO ED Ede af He sop" 4) Write the clinical applications of Hanging drop ‘ ~24 deesminy ta motitly of Has fealetts lop “Dt duce about toa babel! Pegelloe ¢ Tis —casougecten) ~ Tt ita tet round tecbvfpus % Assessment 1 1 [2 i 2 Participates actively and contributes to discussion during SGT 1 2 43 3 Shows professional conduct during the Teaching Learning session 1 2 3 4 Completes the record book activities in time 1 2 5 Shows evidence of learning the new skills 1 28 (Intellectual/Psychomotor) Total score AS (Can be reduced to 5 for convenience) Faculty Remarks/Feedback: Date : Faculty Name & Signature # Mark as 1, 2,3 for ‘Not satisfactory’, ‘satisfactory’ & “Very Good? respectively 32Certifiable Skill Exercise Gram Stain 1) Write the principle of Gram stain pe aennray asgeatin G seal doberrrtus Coluther ob 1y pens fam =ve stofined primes alain anal fixe ae cb er re prlcaasss Aisle Loy ii Pe ve! bs te gt el -v 2 wt Sam staining, si os alse unbier t the microscope, record the observations and interpret \ Gramm -ve each lif Observation Colour! Yiolet d Pini Arrangement ‘ Cluster Shope! splusteat 4 Boculli/Rod dDiséreat Inference > Bt Shows gam tve Cece’ assanged Fig chuster adloug. eoiter fam ve baceitt ariange Pn ctsereate _ Examples : Staply| cceccus pakke Qujerd = E. coll Gram positive cocci in clusters Gram positive cocci in chainsGram positive cocci in pairs Gram positive bacilli Gram negative bacilli Gram negative cocci CO 3) What is the crucial step in the Gram staining and why? Ree_most Crseial Atep 4a _gasec staining is clecclousisation Step o> Caydal be © a < decclowisabion aaent Mleshet Cot) Geelons. “43 Iafren too |. 4) List the differences between Gram positive and Gram negative cell walls "Gram negative cell walls 7) 2 5 2 Grom Postlive batala have a thick cell Wall [Gram - ve backyia bave 2 thin. ob peptdeglycan * Pep tdog lyon layer fn aram ve baskua blo cell Wail ~ ay hay Cube) Merabvene MET “4 “ea Tnembtac Cut iy membiaut tetchale | hageheddpicls 4. bipopaly sacesosichs esti of xéin Piptidegiycan Layo — - C+ bes Single prriplamic (ayes 2 Speut ~ Ze fan 2 pel ee 34Skill certification Performs skill by following all the steps correctly Focusses the stained slide appropriately Tdentifies the structures correctly and interprets. 01 (0.5+0.5) ‘Draws colored labelled diagram of the microscopic field and writes the report 01 (0.5+0.5) Score Rating Rubric Below expectations (B) (Score — 1, 2, 2.5) Meets expectations (M) (Score - 3, 3.5)* CERTIFICATION (*Students should secure ‘M’ or ‘E' to be able to get Certification in a given skill) Faculty Name & Signature 35 | RatingCompetency M1 1.2: Perform and identify the different ¢ Specific Learning Objectives: © Describe the principles of Ziel © Perform Ziehl-Neelsen stain on the s neat labeled diagram with appropriate co! © Identify different acid fast organisms/st able Skill Exercise Cel 4.Ziehl-Neelsen staining ausative agents of Infectious diseases by Zy a iy jhl-Neelsen stain. mear provided, record the observations by ¢,, Jors and interpret the findings. tructures causing infectious diseases by 7 Neelsen Stain © Describe and apply RNTCP grading for suggesting therapy- Exercise 4: 1) Perform Ziehl-Neelsen staining on the given lady with co month, Focus the smear under microscope, rec heat-fixed sputum smear from a 42-yex with expectoration, evening rise of temperature and loss of weight sin ord your observations and interpret the re, Observations seroma Snot les beadect , fud Coleus eel fest boost? angie Pisserce of add feat bacilli a Spulue Sample __——— a sc es ycol chest ables Mycobadesiunn lepraeibe the principle of Ziehl-Neelsen stain » en ee) onedei cell Pippa lal 3) Enlist various acid fast organisms/structures and the percentage (%) of decolorizer (sulfuric acid) used for each type. Mytobactetiun tebesculasis 25% Myo batfetlum Gprae 5%. Necascli 1% Coyplospesteliaen p Cyelossporsy Ziospoe 1% 4) What is Kinyoun ’smethod ? Write its applications, é 4 fram 2 Seht—Nelton Stefolng . - heating ts Ce 11) Phunel Concentration Pn Cathe) Jes fn is Prcveased i) Dualion op Casbol funn sghaiatay ty Gatrensed 5) Write the principle of Ziehl-Neelsen’s stain Corbet Justin Solbbilizer tex Upotelt mateval present In ths myobacituen Cellrdall by tue Appliahioa of Aut Csbol fuscfr. pu nak Gell te % ra tae Cyleplasma op the Cell fenpaslig tual Glos do Hee Cell6) Record the observations of Ziehl-Neelsen’s stain Mleprae tuberculosis Coccidian parasites 7) Explain RNTCP grading Levee Nalana! Tobecilos?s Conbo!_pregemmet =9lva gvidlis. >io/or ¢ We of Baal deen = t-v0/ oie es ag] teo otF -q.(\0o oF af Describe the therapeutic implication of RNTCP grading ent eo D Assessing fapechiouniss of padioat ot) Asseying — Severity of, dhe chisease. 38> ces between Mycobacterium tuberculosis and Mycobacterium leprae. 9) pifferen wy Colhused bet nal rnyea bacletlurn lyonae ti ot — Case i a Lipias elcell_oall add ca Letina tubosiuulais Zieh|-Neelsen Stain ( ti Max. Marks (05) Scored igi attains we di ; I by following all the steps correctly 02 Fone he stained slide appropriately 01 Tdentifies the ‘structures correctly and interprets, 01 (0.5+0.5) Draws colored labelled diagram of the microscopic field and 01 (0.5+0.5) writes the report Score Rating ing Rubric ane Heaton (B) (Score —1, 2,2) Meets expectations (M) (Score - 3, 3.5)* Exceeds expectation (E) (Score — 4, 4.5, 5) CERTIFICATION NO YES (*Students should secure ‘M’ or ‘E’ to be able to get Certification in a given skill) Date Faculty Name & Signature 395, Bacterial culture and identificatig, Competency MIIL.1: Describe the their detection, and di i i infectious diseases and the different causative agents of infectious dise methods iscuss the Tole of microbes in health and disease Used, sAt the end the session, the students shall be able to, sng the bacteria in the laboratory Define different types of culture media (Enriched, Enrichment, Selective, 7, differential) and explain their uses ty, Suggest the use of suitable culture media based on the type of clinical specime, condition a Enumerate the media, methods and uses of anaerobic culture Describe the media, methods and uses of blood culture Discuss the principle of automated blood culture systems with examples and contrast the conventional from automated blood culture method Compare Enumerate the methods/tests used for presumptive identification of bacteria from cult, Exercise 5: 1) Explain the purpose of growing the bacteria in the laboratory , fe M frolotr badaly oom cB rRal and gle appupricy treatment . £ Ly oil Lily Hosea to dice 2) Write the basic chemical constituents in a culture medium Egle Oats fod _dechalydes = Agas= Ads on Aull aged = Yeas! euhact and maalt txbact SSS = Blood 4 Sesumrate different types of transport media and indicate the clini iti 3) itl can be supported by such media © tinal conditions and pst Acrobi 1) VR medfam cholera Vibro cholesa 2) 4 Ble, we | Lateie fever |_ Salmonella +uph?? | 3) |__Goffesed slyeeot Jolie | Gach? al clysenbsy LhPeella spe Pes. ” puteclvel_Seadata | Pattien Exclusto colf Anaerobic: D Antibfobe associated aid hehe | 4 Pehort ptSle. 2 ' ” Rabusten, Goked a | Reed peftcnng dahidlum pe 4) Define different types of culture media (Enriched, Enrichment, Selective, differential) and explain their uses Enriched ; media: Bleed Ages Fast humolyHe propeitly highly fosticleous Sactesta Docolats Agos ke _humppht tes FBolation ef Lesyne baletestam beffles!s Serum dlope Deas Fes Golatuim rmicsobed : Bloool Gutters predlts tion blocel Enrichment Tebertifarch. Brot, fos. S.Typht Gram -ve broth Shigella _deleo the - F- Booth Shige < Alkaline feplora Wats : fos Vy Cholerac: lect = ced lowenshetn~ Jargon meeliuer Myce babi “Tcbulphodt Ghret Bie Sait Sue! fey Vibria Specks - UL Apa 43ON DA Pema BiG 4 Baling Conary ebucteslary Pot Telluside Ages a - a : Heentcns ox mien Differential Mac. Conkey Ages lectose cy ie media: Cyphine lactose eleckolyte Ocbtevent Olteanative 4 elon SR an Macconley Bans a 5) Draw the colored labelled diagrams of culture media (without and with provided below gtowth) in the Pe Blood agar, iy Aloha hemojn Blood agar Blood agar wig, Beta hemolytic MacConkey agar with Lactose fermenting Mac Conkey colonies agar Mac Conkey agar wit Ron-lactose fermentin colonies Potassium tellurite a Chocolate Agar ie with black colored coloniesLowenstein Jensen medium » TORS agar Deoxycholate citrate agar 6) What is blood culture? Enlist the media, methods and uses of Blood culture 7 Bloodlathsst ax eniihed media usted fos Psoletig catego = asgankm fase blood. ; Goutliral Blood (altace = 1) Mona phast medlum %) Biphak medlana. 8) Automnbed» Blood (uluce — Bat /ALERT sed fo Gltuce myto bacteilem Hbaesteste 7) Enumerate the automated blood culture systems. Add a note their principle d Bacardi 20 ji) QcP/ meer VTeTvo WWQACTEC + GacT/aeeT Ao 4 ALERT vretvn = i2esk tated an colos charg? _ hhe=gisen ae la, precluclian bi, Baedsiln lracliasy sh clonge (a OH * Ske os Aenlie Howsoscent dijo, da slibecl ooictis al bach. 45> 8) Discuss the advantages and limitations of ‘conventional and automated blood CUltug Blood culture system ‘Advantages Limitations Conventional ailay way ot ae ~Soben thay real Poa beleat Blood Colles | = Cen Weary oy sewed eatit in Comp Fd ‘Automated Cold 4 rote osilve high Coat oH el = Lys labo intense rabid mares a 9) List the anaerobjc culture media el 10) Describe the anaerobic culture methods and write their uses. as ° fi Pe ie vp_O: sti love fee stdin setacy —_Subthaws Ohta fate wp, Caects Loos felor ploy Ltt pail tts ole ppcbligat, Arasobes ser © clashidiaro = Malnlainecte of Stoik Cull Postel X- burnolysis Preswrnocece? idlian Shepteceres dpe “# - huey em Shyphecoees Propet 7 No hme srs Enterceceess Naytobactrlun Geen _colous Colony lacs 4612) Enumerate te biochemical tests used for presumptive identification of bacteria Cadalese Test ondase Test sable Hel lole Cth hen hyde lysis test 82 Tat Cooguleve 102 Hecd _tolesance test BPR Solsboylily est amp tet Lnvlln feamektin fest Ant miso bial Susceptaeilty Tes’ 13) Draw the colored labelled diagrams of key biochemical tests (Positi ive) i ae provided below and write examples ae a) Catalase Test c) Indole test b) Oxidase test d) Citrate test 47€) Urease test 1) Mannitol Motility 14, “ty y g) Triple sugar iron agar Gea) Cows) Gated) Cun A Ayo PU EU 14) What is MALDI-TOF? Discuss its clinical utility in diagnostic bacteriolo; filed oy Portcabion time ean : ‘ Give fapornation Ghout tve _ cnictobal gia thy aot help deakbiakOr af. baci : 486. Antibiotic sensitivity testing Competency MI1.6: Describe the mechanisms of drug resistance, and the methods of antimicrobiay susceptibility testing and monitoring of antimicrobial therapy Specific Learning Objectives:4é the end the sessions the students shall be able to, «Discuss the einial infcane OF drug resistance in bacten. «Describe the methods of antimicrobial susceptibility testing in the laboratory «Enumerate the antimicrobials 0 be used against Gram positive and Gram On of bacteria © Interpret the antimicrobi ts and suggest therapy ial susceptibility test resull Exercise6: 1) Explain the significance and methods of antimicrobial susceptibility testing in the labo, a 4 Bectole Exhibit eat siail lation So__Susceplile bo _ankienlacial aserk pike tue clnifan do Loring Hor \ -pasiles_ 2ST pny flalsele 40 eaetad Von to ; r T A The Heseronetaed, clilubioo Tot [Biola Baas ciluHon VITEK , puss ‘ > Opi! a5 fin Linisso gat Bulomobed psp Ee 2) Describe the principle and utility of Kirby Bauer Dise diffusion test enost ddely sed Ast “They att Gocaepadle Seer Suitable few Sopdly geosing pathmedte. batten TF 3 ed fa lay ale Celony-D0 64) pesbosmes! ie ete 507 Jed diagram of Kirby Bauer Disc diffusion test with inhibition indicati 3) Doe resistant (R) and intermediate (1) results, sa ones of hibition indicating Resistant (R) ‘Sensitive (S) Intermediate (1) 4) Waite the recommended antimicrobials to be used against Gram Positive and Gram negative bacteria Gram positive Cehoxt An Cipro Hexacin » Chlosamphastect Cefa zeltn. Gram negative Arenpldlita- Clavulfale acid: Cebe 20lfoy ofloxagn: 5) List the first, second line and the reserved antibiotics used against gram negative bacilli Prnplatlin Cipro oxen Arora din = Clavdlinte asta: Cefticmon: ca Gueloperorasore Cefepime Oipaa clllin-—T Aen? leadn Reserved Menopenun Ente penum: Doct pmm eS penun 51™ 6) Explain what do sensitive (S), resistant (R) and intermediate (I) mean in the antiny, susceptibility test reports? : i Sensitive (S) Eandeace a ee SS Resistant (R) sa nat CHeoieally elfectve tan TTI Intermediate (I) Palibate net effective tm Blerdad 4 llectve ics aie > tS ea Ty, Describe the principle and utility of Minimum oe Concentration technique On =b Based on dilution e 4 ee 8) Describe the principle and utility of Epsilometer/E-test. Ay de guablah ery of borott ba of Antibiotle Sante tes medica 9) Drawa labeled diagram of Epsilometer/E-test 5210)Enumerate the soa apres available for antimicrobial Susceptibility testing stvily test i a 10 2. “wn Assessment Score” Comes prepared with requisite prior knowledge 12 2 Participates actively and contributes to discussion during SGT [1 |2 3 Shows professional conduct during the Teaching Learning 2 session 4 Completes the record book activities in time 1 {2 5 ‘Shows evidence of learning the new skills 12 (Intellectual/Psychomotor) Total score AS (Can be reduced to 5 for convenience) Faculty Remarks/Feedback: Date : Faculty Name & Signature # Mark as 1, 2, 3 for ‘Not satisfactory’, ‘satisfactory’ & “Very Good’ respectively 537. Indirect methods of disease diagy, Competencies MIL.8: Describe the infections MIB.15: Choose and interpret the results of the laboratory tests used in diagnosis diseases (Immunological tests) y mechanisms of immunity and response of the host immune Yen, b the: Specific Learning Objectives:At the end the session, the students shall be able to Exercise 7: Preciftation Reackon Enumerate different immunological tests available for diagnosis of ines, Describe the principle, types and application of Agglutination test ' Describe the principle, and application of Precipitation test Describe the principle, application of ELISA test Describe the principle, types and application of Immunofluorescence tex Discuss the principle, Rapid Point of Care (POC) tests with examples, 1) List various types of immunological tests available for the diagnosis of infectious di Enajrs Unkel Senmuns Seslen & Desay Arplleabion Recckon Eryn [foked lowescent Assay, Complement FR zatten tes) Tmmone Hourescence Aasou, Neuhelizakion test Gharfhuniin fgcence - vee al, eon a 5) Enumerate various inclusion bodies and indicate the viruses producing them,6) Whatis the principle of Polymerase Chain Reaction (PCR)? Discuss its applications. Pascipleof |) one Ertan bora tha exganiaen 2) amplifeabion of Extracted DNF 3) Grd clechophoro sts Applications ni ves 4 ny con detect “ohutlss Culluse ia Fesshilious (ox) Non Jestidiay Assessment ‘Comes prepared with requisite prior knowledge i 2 Participates actively and contributes to discussion duringSGT [1 |2 | 3 3 Shows professional conduct during the Teaching Learning 1 2 a session 4 Completes the record book activities in time ry 2 3 5 Shows evidence of learning the new skills 1 2: (Intellectual/Psychomotor) Total score ‘AS (Can be reduced to 5 for convenience) Faculty Remarks/Feedback: Date : Faculty Name & Signature # Mark as 1, 2,3 for ‘Not satisfactory’, ‘satisfactory’ & ‘Very Good’ respectively 61y ‘ 9, General principles of Laborat, diagnosis of parasitic diseases Competency MI 1.2: Perform and identify the different causative agents of infectious diseases : ZN stain and stool routine microscopy | i ane Objectives:At the end the session, the students shall be able 1, Se ee oud steps in the laboratory diagnosis of parasitic infections i Discuss the types of clinical specimen used in the laboratory diagnosis of par infections : ic . ienose the underlying parasitic infection based on the history and clinica) ym and suggest the suitable laboratory test in the given case. 7m Perform saline and iodine mount from the given stool sample and identify Vatio morphological forms of | parasites. us «Identify various parasitic forms in the peripheral smear. © Describe the role of immunological tests in the diagnosis of parasitic diseases, Exercise 9: 1) Describe general steps in the laboratory diagnosis of parasitic infections D) Exanfoakon of Feces 2) Specimen Coilechion. 2) Milks Semple Fexarnins ion 8) Contenbralin echnigyy 2 ) 6) Nicleuuloy Methods: 2) Describe the collection of stool specimen for parasitic examination Stoo) Coilecl Dhould be clon ina ¢Oids mouthed clean leate _Fockas Reguised tn Collecktom = “Pinging Frequent = Exanfnalion 62> different types of clinical specimen (other erate ceo 5) forse w ections and indicate the clinical conditions stool) used for diagnosis of Specimen Clinical condition/s net 2h nou clits matesia parasite —_raigofileaoe’ Gleod_Sereas’ Bloed pasasibe eee Examinakon Mmolesia _pososite ' en _Boiiloedy clefertion: Tryponaserma 2p .s. om GF Draw the colored labeled diagrams of common parasitic form seen in stool 4) > sheongylBdes ti . Ss coast of 22 cyshosech® » we gai eae A Nv a _\__, Brstocysts ay (folie rout 635) Draw the colored labeled diagrams of common parasitic form seen in Petipheray hb Nl oN bog % Moltest . Cees fownetion OQ Rn eSreic: BeloeSasis Taypernastignle ta “rypanosonfogis \ 6) Enumerate immunological tests used in the diagnosis of parasitic infections and their applications. Immunological tests ‘Applications |) Antibody dubediontesF Plagasis ef Arnoeble liver Aisead € as Teropatmeas » CyH cenafe, O enoay > Visceral telshmnaniosis ©) Saboin~ Feldman dyelesy | —>Texs plosmesis > Anbgen deleckion tesb o) Ecrsp — Ameebiesis, tymprale Gllaslas?s 5 re K> Giesdia 2 -Weldy hee Cry pho 8ponidlin Malesia, Iyeatial bilasicw 1s- —U— Cel ble Skill Exercise Exercise 9.2: Stool Examination A lady aged 22 years complains of abdominal discomfort and altereq cone week. She has submitted stool sample. Perform stool examination bowel, elements. Record your observations and interpret the results. © look fi tr Pal i » Observations Bay 3 Romd 31 USuim Stee conkt oh an embryo ufth —¢ hoods Susounded by on Embropar leskire Inference Fay of Tien eh a bite Bk 665 of Laboraton, ciple i Of ip al diseases 10. Ge eros of fung? inet ys diseases and the method ig of infer g disease come? etre ister oe ie sit ha 11: Deseribe en etecti0™ and discuss sion thet udents shall be able to, session Wr vestAtt eend 0 of viral infections eaned byt spt re ing 0 ns ory OF sod te snfectio! S m puree meior rdentify meio" Discuss gem sfture me mmon fungal ce methods for fun a een the diagnos ° Exercise 10: for laboratory diagnosis of fungal infections 1) Explain the need ‘srt ise) ‘nosso diagnose odiscase — > ahi r a ont 2) Enumerate major fungal pathogens and the infections/diseases caused by them Fungi | Infection/Di i i gi fection/Disease caused: Fungi | - Infection/Disease cas’ a Aspuctlts Histoplsins Holopkimss Penattum ie | P 8 oi eta ddd enallets Jostorny ced Legho mycosin en cols dlaimolikis a aoe Plow ceesis Orie — | Cecaid toida Gevidte oasis [Mathias Godidicnys saad ona a mycdomaty | Mucebome 68colored labeled diagrams of major morphological classes of fungi Draw Yeast ] Yeast like oe | Dimorphic Write the common fungal culture media and their uses 4) Mest Commonly wed in oly chulro se Agas diar! diagnosis of mycoley | Sab ouroncls chskro se Ag col -4 Rich Blatch Age Chlamydogpore_produchion Gane Crrersing Fosbidious (oagt Gada lust! Gofusfon 4 Blood ages 7 an niga teed 4 Bind Seed Paes _Gelerkive poe Couple enceus Uv 697) Drawa labeled diagram of the microscopic appearance ofa mold in LPCB mount.; ‘ ; differences between laboratory meth . : 8) Wi el infections ry methods used for the diagnosis of fungal infections Fungal diagnostic methods Bacterial diagnostic methods | | py - KOH pre paahion grarn- to Direct t ee arene ical roi fa, Cathar Condttions a d 2 cole Shite 4 Culture redfa, methods -caloay smespl legy + yp aunkffeaton Iv tdiphic abien |_ 2 Steamunalsgtt enethods 4 Antfmisobials Juscepbabilily tect paternal i + Sesolegy -¥ Gpecimen Collection Hons |__ sa nteleot eptthods. _]=1 Wcleaulot enetbadls Typing em theds. | ‘Assessment [SENo. | Student’s performance : Score™ e - Comes prepared with requisite prior knowledge 1 12 T 3 owed Participates actively and contributes to discussion during SGT __| 1 2 \3 | Shows professional conduct ‘during the Teaching Learning 1 2) 3 session 7 Completes the record book activities in time 1 2 \3 5 Shows evidence of learning the new skills 2) le (Intellectual/P: 'sychomotor) Total score AS (Can be reduced to 5 for convenience) Faculty Remarks/Feedback: Date : Faculty Name & Signature F Mark as 1, 2, 3 for ‘Not satisfactory’, ‘satisfactory’ & ‘Very Good” respectively 7=— tae WHO's my five moments for hand hygiene. | of be eee ig a " a 9 J 4 , What are the products used for hand hygiene? Fe ai Tr pinkn Sn Plastic Apron — Ansnqess0 hand hygiene and ene use? ible Conlermehey 3, What is relationship between bo 4, What is fit check and discuss its significance? Jp ossust Hot tte vrai ls ond Sas propaly 26 polealll “at atc fond inanlaa Qulosbone) ots Kept Qube 5, What are the precautions to be followed while removing a mask? > DoltSng de not b douch “tis Bont past ote mnaate 2) vine dha kool Hot, tan ee vps toed ot Ramove aa - ha ; od ove! 74> 6, Name indications where hand wash is necessary and hand rub may not be effective isnot ood _(O% " = : assessment Student’s performance. ae [Score ] Lgeihinsieti shia tins tnd : ‘Comes prepared with requisite prior knowledge 23 Participates actively and contributes to discussion during SGT 1 2 3 Shows professional conduct during the Teaching Learning 1 {2 session ‘Completes the record book activities in time fe 3 Shows evidence of learning the new skills 1 2 3 (Intellectual/Psychomotor) Total score AS (Can be reduced to 5 for convenience) Faculty Remarks/Feedback: Date: Faculty Name & Signature Farkas 1, 2,3 for ‘Not satisfactory’, ‘satisfactory’ & “Very Good’ respectively 75Exercise 12.1: 1) List the clinical applications of steam sterilizer in a healthcare setting " th mh zd Rostsbent $- Susgieal %ndeau: ; ee al Tata aginllggss Pn Rol tet Da a List the clinical applications of ethylene oxide sterilizer in a 1a healthcare setting bali ibe) H labile = burg machPar eee J tu ctronfe 5) Assembled Complex clevices_ 6 Mult lumen tubh ee 3) the clinical ee of ae sterilizer in a healtHcare setting estan 4 le Alla —AeSilhabion asia =a Seille Latage itor 5) List the clinical applications of membrane filter in a healthcare setting 1) Filhalion of ais — fn duet mask avd Spfiales ; Tn ECA filest sedi blosfely Cabin i) Fillabinol, Ugetel > = Los Blolesial sxaumina lion of cecbs fn bespttal » 6) List the clinical applications of dry heat sterilizer in a healthcare setting ihtzabe Pol thet fs 05 thet cae Pepinehable te mdit bead Far Golascase”, pocoles 7 pelsclewn pecclucts _p,jLasp jxstranvits 7, List the clinical applications of glutaraldehyde in a healthcare setting ‘infection L= opdetiyy 8. List the clinical applications of alcohol in a healthcare setting Asiofechon off Smalls non cfbial shuren Sushas Har rnornetey Ate hosed hand gu - SPSL. Ext Surfaces of Egdpment Sechag - Stella Spy Venhiloless, Uta pncebine * Smaller Phen s = (ublees Beppe’. — 81 yitheare setting tae Ore ink ee een or ae alters oe set Sei ae oily ca ie al ful ine ae 10. List the clinical applications of povidoneiodine in a healtheare setting a P é ke hand x =A Ee = - toy fisia sub — 1%. -asal Got Sepbic £220 bh psdeni 11. List the clinical applications of Quatemary ammonium compound (QAC)in a heal, setting heare 12. Choose appropriate chemical disinfectant/sterilant is in vi [SO [CisicaVLaboratory situation i. re aie 1 Busing emeganey @h) ~otlonged Flesh Stest lization 2 lapescecope, Spine set Plesma stesTizahen 3 Cultus media preparation Rutodove Dental 9 taunnents PA - Relic acicl: = Swgiel sin = prepenkon Lod ne S mouth asin Cintas be oGdeew z Canrock Lang LW Roliohiary 13. List the common bio1 medical waste items generated in healthcare setting that are segregated in yellow bag. Haman Analormieal Coste Adicoal Anateital clscctded meclSro Cherbeat Soltd weske, Li 5-1 ns ~Trfecteo! beg dels dicated Asiopeclante discret so Coatamin bed 9b, GBlogl. Body (eds ALS waisobidesy ottus labcnah blead bags Mee alberrcted jercofres 14. List the common bi ‘medical waste items generated in healthcare setting that are segregated in red bag, Plepsable Pte Lach os bubf, be ettletes LV tober ¢ Seb Clesterd nite a Ay waged rMbeeed- thes, Vacisdafact 6 Jove flab pion» lek 82> \ . on biomedical waste items generated in he 15. List the proof white/translucent container. — in pune Reeckles, Need tulle | ki nang, EER a et bao on Itheare setting that are segregated ;t the common biomedical waste items generated in healthcare setting that : So ot container. wae in 1 se = Bien Lh dlscatled , Cortuminatee! glass Praludh ine Viale, enol Armpouls to he te calle becky Prnplants 16, Deseribe briefly the steps of management of blood spill in correct sequence : Jpege should be attenelid Soronecably | - Dh y Arvoel | a it __bppothosibe. = Alles Conterct time f - Kise with t el 17, Name the components of the respiratory hygiene and cough etiquette? = Crsschty SneesSagl 2B baacl pibalbiled. | . meat, Gvesed co fs Hest | > Qywe saith Clan wate = Allow Contact tinue of lOmlaks ; | 18, Name the methods used for assessing the microbial contamination of water? | tb id Samples dots , lasge Volums | ' altky ab. clifalaig. Dabs | 19. Name the methods used or assessing the microbial contamination of air? i }_farsive mort toring (Settle phtd) rnethed | Dadve rnasthosta (Sik plate) onethed i “2)_Ats paste Cou ntest 20, Enumerate indications for performing environmental surveillance in a healthcare setting? = Osbbrese Fnvestiahion = Evolate dtr 4 change ta Infection | = Lprthuction 4 Sisensch puspost \ 1) mivibiple tube metic = fox difoviue rosbs analysis and fos high 832 Exercise 12.2: Needle stick injury. Analyze the case scenarios provided and’ discuss measures to be taken to Preven ick injury in each of them. t infection(s) due to needle sti = i “iy A. A 32-year-old staff working in biomedical waste department reporte, , complaints of needle stick injury while segregating @ yellow bag. The ine to i hours back. He did not perform any first aid measures at the time of “ident Ce received any Hepatitis B vaccination before. Discuss the post-exposure pr, injury We My that should be undertaken. Phytaxs, : t+ deseo B. A 28-year-old medicine resident aint \ ¢ i it reported to HICC with complai: eedle sti his left thumb while recapping the needle. The incident paras bel ce ie - One mentioned that he i i i injury, \¢ had immediately washed his finger with running tap water for 2 u12"* (Or ie source sample t iv hi ple was tested and was found to be positive for hepatitis B surfa: -_ ICE antip: and reactive antibodies. The resident had received a complete course (o1 e for HIV antibodies. Th id le (one hepatitis B vaccine 10 ‘ years back, followit i i Discus: > ing which he had 7 . series) s the post-exposure _ prophylaxis " i file a = 550 ne e undertaker Tt He Exped a : pissed is Completely Vaccinated + 5 a Sete ee deslencd ae Pea —ok wiof Hu Seusce os ob tis She Taisented to HICC with complai i inj i 1-year-old surgeon repo! mplaints of surgical blade injury on his left c. Aaly hr back. The source sample was tested positive for hepatitis B ‘surface antigen, but : HIV antibodies and HCV antibodies. The surgeon had received two doses of itis B vaccine 2 years back. Discuss the post-exposure prophylaxis measures that should ‘Assessment ‘SLNo. Student’s performance 1 - ‘Comes prepared with requisite prior knowledge : 2 Participates actively and contributes to discussion duringSGT [1 |2 3 Shows professional conduct during the Teaching Learning 12 ie session 4 Completes the record book activities in time 1 2 |3 5 Shows evidence of learning the new skills 1 2 3 (Intellectual/Psychomotor) lt Total score AS (Can be reduced to 5 for convenience) Faculty Remarks/Feedback: Date : Faculty Name & Signature # Mark as 1, 2, 3 for ‘Not satisfactory’, ‘satisfactory’ & ‘Very Good’ respectively 85> sercise 13.12 ; ical case? . Ciinier old male presented to OPD with fever and chest pain while breathing, He had a soye? tory of cardiac surgery for valve replacement. No H/O smoking or IV drug abuse. sent for culture sensitivity. ig : TT Hesloseenent Seen, 2, Name the common etiological agents of this clinical condition. OT shophugacocens Psteut, —Shreplocees’ _, Enbutncecd’ 4 Pruuens cocci 2. e 6, h 3, Describe the diagnostic criteria used for this condition. edodified Dukes Csthosfa faa clfafral cling anits of Tafecbive 2 Cabclitis Dasfos Ce wife = eve Blood Culluiw, Eviclrce af Enclo cavltat favdvernut pies Gfleta - Predisposition, eves, Vaseuiles phencmnenen. 4, Describe the procedure to collect specimen for this clinical condition Le & Wve O4danfs N bey CRIM eas —for_cliagansh clitevmfrahoa of AST ane plinnfup of. treateasat 00 Blood Gulfutes Gollecked at ‘nfetval of 2 12has b)u) It amd Sef 5. Suggest treatment for this condition |e Reg ene fos_ Auteu —LE . fos Nate Valve TE = fos NIRGA — Gorfirmelory chiag nestsAssessment SLNo. Student’s performance on i 2 Participates actively and contributes to discussion during 3 4 5 Shows professional conduct during the Teaching Learning session Completes the record book activities in time Shows evidence of learning the new skills . Antellectual/Psychomotor) Total score (Can be reduced to 5 for convenience) Faculty Remarks/Feedback: Date : Faculty Name & Signature # Mark as 1,2, 3 for Not Satisfactory’, ‘satisfactory’ & Very Good? respectivelyExercise 14.3: Clinical case: 45yr old male agricultural labourer presented to OPD with shortness of breath for 2days, with dry cough, high grade fever, swelling of both lower limbs, decreased urine output since one week. O/E bilateral maculopapular rash with Eschar and lymphadenopathy was observed. Bilateral crepitations in lungs +. Weil Felix test is done. 1, What is the clinical diagnosis and how you arrived at it? eo Ss = 2. What is the etiological poe and how it is transmitted? oo Le. ° : caftes - 3, What are the clinical manifestations seen in this condition? hosiness of, b day Cough , Feves np o 2 limb nel yn phacleno pe Hayy 4, What are the various modalities of laboratory diagnosis? _i) well Felix test 2) Zndtsect fomunoflausoStente Assou a) ELISA -u) Per. 5. How will you treat this condition? Drugs like = 1) Dosyeyeling 7Exercise 14.4: ed in the hospital with C/O high grade fever, Clinical case: A 15 yr old boy was admitt ‘d rashes all over the body since 5 days. Headache, Myalgia an Lab parameters showed d Tourniquet test was positive. Thrombocytopenia an 1. What is the clinical dingnosis and how you arrived at it? TTs_patfend 9 Dengut Hem a Fev, ct pollen? —oith 1 aw hy o% List the viruses that cause hemorrhagic fever. : 3F a = Ex- wwe_V) ellourteves _vPhus Lovie — x= febala 4. Sus-e9 Hantav' Arem Asoo Raut Mi = What are the clinical manifestations seen in this condition? 4. i item the various modalities of laboratory diagnosis?. ae eo. chet yy etisaand fat 1 Antibely debechion by Yeu hol? lon tes VF Sus fcolation by noes a (Molecules ~ Realtine RT pee elects Spest dic ae sata late calf a F Visa I RNA a How will you treat this condition? placa je lah J soe ee 98> ™ gsc a diagnosed isi oa male who is diagnosed with HIV with 3years back was admitted in th aa ee ‘vith fever hypotension altered mental status, 3 months back his CD4 count yori dwas not responding to antiviral therapy. wos low sin RR is 30 per min, BP is 100/60, temp is 102°F oe east cells are seen in Gram stained smear of blood culture. pu wwostis the clinical diagnosis and the likely etiological agent? 1. tdtoals: oo (Pefensts Co Krusel, C. Ausls Name the risk factors predisposing this clinical condition. Po Bi Fast = eee A as Pregnancy = loo Pearmuntly ! = Sol t OM 4 Pron @s) Hine defiefency, 3, What are the other clinical manifestations caused by this organism? © ge0_hypakeas Son, albesecl mental Stalus Tetispante to —_Abivfial Ebssepy¢ 4, What are - various modalities of laboratory a , yoren +Y4 Coltue — Caen bet Posty Coloo% Delman plot “bihs- chlamydospe ts: Cmmencdiagnosis , BJ Grlvcan puss) | 5, How will you treat this condition? ! ~ Cataneoss Candidiasis —_— topical Azole | “Biopingea\ —Candidtan's = Fluconazole / Cospoly “Disseenlnabed) Conc\idiasis _—_Cposormal aopfindl /-Cospeingin) ) vestenasstd 99oratory diagnosis of Enteric fever enteric fever pathogens and discuss Wigs 5 De ory dngnosi oe AGe Be de thoes td of the clinical oo the the different me ities for diagnosis of i enteric fever. Choose the ae «oe rnc tte duration of illness «ties One the st dents shall be able to, scat ‘a ema ‘sms causing enteric fever ae manifestations of enteric fever aoe i fr dag of om ; jate microbi investigation for . coo appr ‘ods of enteric fever = : 2 , t pene patho, ratory tests done for the detection of enteric fe ie laborent modalities of enteric fever eiaee + Des 5: pis tay old boy is brought to OPD with C/O fever abdominal gst age: A L2yr OV Od step ladder type of fever, O/E relative aay : ce 5 days a i oot plenomegaly was noted. Blood cells for culture and sensitivity and Widal sl fst clinical diagnosis and how rons aati at it? Wats oe tgough tte ta = Abd, g on 1 Hows disease is transmitted folk: Eee id ey, tisough Hts Feaco oral Rouk E. 4 Whatare the clinical manifestations seen in this condition? cies ip = Kase Spols: 101o . 4. What are the various modalities of laboratory diagnosis in different Periods OF the di, S.No. Period of disease | Useful microbiological test vy ka PE Sy ene ji 1 week Culkuse of = Blocel ; faa sum — : 2 week : For Aad becky clebechion by UdVdel tes} i 3 3” week Sau Ty Lage o oe 1 Bhool £ sine | 4 4” week | _ Sfoolt ¢ UsFne . i \ a eee a6 — 5. How will you treat this condition? 040, 2. ° t 1h | + Probenedd fos, 6 Deeks. 6.Define titrewhat is local titre ? Titve= Te highest clilulfor of Sete sab cobtetr cpp Halle essa 2 4 1216 a F . 7. Interpret the Widal test results (Titres) S.NO. J S.typhi | Styphi_| S.paratyphi]_Siparatyphi = =. = Ynterpretation =a aie} -3-O. H AH BH x | 1 20 40 <20 <20 | -ve.- 2 160/320 | <20 <20 tve for Sclmmonella Tyrht | 3 320 | 40 640 <20 +46 bok Salona 4 160 | 40 <20 <20 Recint Prfelon Oral | 5 80 320 | 160 160 VacSration . | 102> amnestic reaction . Assessment with requisite prior knowledge Participates actively.and contributes to discussion during SGT Shows professional ‘conduct during the Teaching Learning 1 [2 {3 jon Sees the record book activities in time A2e 2 Shows evidence of learning the new skills 1 {2 {3 (latellectual/Psychomotor) L Total score AS (Can be reduced to 5 for convenience) fealty Remarks/Feedback: Date: Faculty Name & Signature # Mark as 1, 2, 3 for ‘Not: ‘satisfactory’, ‘satisfactory’ & ‘Very Good’ respectively 1036.Laboratory diagnosis of Malar, Competenci : - MIDS: Describe the etio-pathogenesis and discuss the Clinica, : laboratory diagnosis of malaria : Volutiy, . MI2.6: Identify the causative agent of malaria n y \ Specific Learning Objectives At the end the session, the students shall be able to, * Enumerate organisms causing malaria * Describe pathogenesis of malaria * Describe the clinical manifestations, laboratory diagnosis and treatm, types of malaria : i ENE of i * Describe and identify diagnostic forms of malarial parasites in Peripheral by th * Interpret laboratory tests to detect malarial parasites loog Exercise 16.1: oe Clinical case: A 30yrs old male was admitted in the hospital with C/O fever chills rigors since 4days. He gives history of high grade fever every 48 hrs and fever sy," withprofuse sweating. On examination he had tender splenomegaly, Peripheral tty smear examination was done, loo 1. What is the clinical diagnosis and how you arrived at it IP 6, : ( : = Eelacie Paso mu, aay high gtads Foes cyery URbs) na Piast Bile 2. What is the host, infective form, and mode of transmission of the parasite? hos — Ferole Anopheles 4 fotesmedioh host Man » Go = fi Ties of Ersotmfssion = peasquiln bike Gs Bled trolasion (Raa) 3. What are the various complications seen? Cestbral _wiolaio_, Peantfous rrolefa , Black cones reves, Aleid inutile . Zepticemic rales Pulrnonasy distress 4 dome Rea: i 2 104savious diagnostic modalities? the ae 0 A ee ake ee 2 js clinical condition? ee ea 7 o———A——e henfistntn > petted diagrams of common diagnostic forms of this parasite focused tal oom > Ring forrnof. plarmediom Ni vot v sniont oF plasmmed on Viva 105Exercise 16.2 Clinical case: A child was admitte: 4days. H/O fever every al Peripheral blood smear shows ring d in hospital with high grade fevervomitings, abdomi, Iternate day and splenomegaly was noted on auinal forms and trophozoites of Plasmodicn ma Viy, a it? 1. What is the clinical diagnosis and how you arrived at i \ modiam Viyare: —Chinteal chi = aa i ¢, : : Sa Se 2 cae is the host, infective form, and mode of transmissionof the parasite? a ———————— e n 3. What is relapse and how it is different than recrudescence? tn hoarmnnt 4. What are the various diagnostic modalities? > Pulphusal Face! Bren => kavamete!s technique + Qventahive gully Coat Exoretrabior - 5 How will you a this clinical condition? = tl : Relapss fea 106oratory diagnosis of Filan; 17. Lab : Leishmaniasis ASig Shy Competencies ‘ jo-pathogenesis and discuss th y Mi2.s: Describe the etio-P: Sate © Clini Isborsory diagnosis of kala-azar, filariasis and other common Pane von. MI2.6: Identify the causative agent of filariasis ites rewtion q len ae ti fic Learning Objectives nih ae ond the session, the students shall be able to, ‘ \ «Describe the clinical manifestations, laboratory diagnosis and treatm, + Describe the clinical manifestations, laboratory diagnosis ang treat Of kay filariasis ent op e collection method for laboratory diagnos; 7 ™, sis of fila: 7 ee + Suggest appropriate samp kala-azar + Identify the diagnostic forms of organisms causing kala-azar and filatiag; ig % ss Exercise 17.1: Clinical case: An adult male from Bihar was brought to OPD with-C/o feve, r days.On examination he is pale with hyper pigmented rash all over the bo, dy 288 sin, splenomegaly. Bone marrow aspirate wassent to lab and Leishman stain ig ae ten ac! : ay 1, What is the clinical diagnosis and how you arrived at it? Clinical liegneis = _vfsceaal leishmanfs — Kala Aros = Frye), Ragin” ince WOdlasys- = ‘npaspageoen ed Roses 2. What is the host, infective form, diagnostic form, and mode of transmission of the Parasi te? hast ia mon Polective asm= Promoshiante form. Mediof byontimision = bike of Ferol SondPly — Phlelocta mus: 3. What are the other diagnostic tests to diagnose this condition? Mieresen py = (nfemse BP Pnm + “ = NNN mediuen, Schniedesls ‘ Prep e — LefShmannintest — Montes tes dat 4. How will you treat this condition? —Fintavolent Aobirnorials = 20.04 Na fduy fos socdaus =HMilte feslea = soma ld Loa i 108